Sed0017335 (gene) Chayote v1

Overview


Name	Sed0017335
Type	gene
Organism	Sechium edule (Chayote v1)
Description	cytochrome P450 81D1-like
Location	LG05: 45280564 .. 45281578 (-)
RNA-Seq Expression	Sed0017335
Synteny	Sed0017335

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTAACGAATCTGACTTGTCAAGTTTGAGTTACTTGCAAGGAATAATAGCGGAGACCCTACGGATAAGCCTGGTTGCTCCTTTGCTTGTACCGCACTGCGCATCTGAAGATTGCAAGATCGAAGGTTACGACGTTCCACGTGACACAATGGTATTGATTAACGTGTGGGCTATACATAGAGATCCAAATCTATGGGAAGATGCCACTAGTTTTAAACCTGAAAGGCATATTGGAAATGGAAACATTGGAAGTGAGGCATACAAACTAATACCATTTGGGTTGGGGAGAAGAGCTTGTCCTGGTGTGGGAATGGCTCAACGTTTTGTTGGCTTAACTTTGGCATCATTGATTCAAGGCTTTGATTGGGAAAGAGTGAGCACCTCGTTGGTTGATATGACTGAAGGTAAAGGTCTCACTGTGCCCAAAGCTTGTTAGAGTGTTTAGATTAAATTAGGATTAATGTTGATTAATATGTTTGTTAGTGTTAGAGTATGAGCCCATTGGGCCTTAGGTTAGGGTTTTGGTAGCCTATATATACAATGTGATAAGGTTGTATTTGTGGGTGGCACTATGTGCCTCCATCATCATCTTTCTCAATATAATTTCTCTTTATACATTTTCATGCTTCAATGATTTTCGCTATGCATTTCTATACTCGCTCAAAGCTCAACCTTTGGTAGATAAATATAAGCCTCGCCAATCATAAAAGGTATTATGAGTGTAGCAACTGATCGTATTTAAATATGCAACATTTTACTAAGTTGTATATAATATCCATAGAATTATGCTTCTTTTGATGCTAGTATTTGATATCTACCAGTTATTAACTAATGTTACTATATAATGTTCTCGTTTGAAATAATGAAAATCTTACTTTCCCTTAATAATCTAAGAAAATGACATGAAATGGTCCCGTTTTAAATTATAAAGAGCTCGTATTTTAATCAATTTGAGTGCAAGATTCTAAAGTTCTTCTTTGAATTCTTCAGATTGGACACTACAGTTGACGTAA

mRNA sequence

ATGGTTAACGAATCTGACTTGTCAAGTTTGAGTTACTTGCAAGGAATAATAGCGGAGACCCTACGGATAAGCCTGGTTGCTCCTTTGCTTGTACCGCACTGCGCATCTGAAGATTGCAAGATCGAAGGTTACGACGTTCCACGTGACACAATGGTATTGATTAACGTGTGGGCTATACATAGAGATCCAAATCTATGGGAAGATGCCACTAGTTTTAAACCTGAAAGGCATATTGGAAATGGAAACATTGGAAGTGAGGCATACAAACTAATACCATTTGGGTTGGGGAGAAGAGCTTGTCCTGGTGTGGGAATGGCTCAACGTTTTGTTGGCTTAACTTTGGCATCATTGATTCAAGGCTTTGATTGGGAAAGAGTGAGCACCTCGTTGGTTGATATGACTGAAGATTGGACACTACAGTTGACGTAA

Coding sequence (CDS)

ATGGTTAACGAATCTGACTTGTCAAGTTTGAGTTACTTGCAAGGAATAATAGCGGAGACCCTACGGATAAGCCTGGTTGCTCCTTTGCTTGTACCGCACTGCGCATCTGAAGATTGCAAGATCGAAGGTTACGACGTTCCACGTGACACAATGGTATTGATTAACGTGTGGGCTATACATAGAGATCCAAATCTATGGGAAGATGCCACTAGTTTTAAACCTGAAAGGCATATTGGAAATGGAAACATTGGAAGTGAGGCATACAAACTAATACCATTTGGGTTGGGGAGAAGAGCTTGTCCTGGTGTGGGAATGGCTCAACGTTTTGTTGGCTTAACTTTGGCATCATTGATTCAAGGCTTTGATTGGGAAAGAGTGAGCACCTCGTTGGTTGATATGACTGAAGATTGGACACTACAGTTGACGTAA

Protein sequence

MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIHRDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQGFDWERVSTSLVDMTEDWTLQLT

Homology

[Close]

BLAST of Sed0017335 vs. NCBI nr
Match: XP_022985609.1 (isoflavone 2'-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 229.9 bits (585), Expect = 1.3e-56
Identity = 104/135 (77.04%), Postives = 121/135 (89.63%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           +VNE+DL SLSY+QGII+ETLR+   APLL+PHCASE+CKIEGYDVPRDT++LIN WA+H
Sbjct: 333 LVNEADLPSLSYVQGIISETLRLHPAAPLLLPHCASEECKIEGYDVPRDTIILINAWAMH 392

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDPNLWEDATSFKPERH      G+E+YKLIPFGLGRRACPG+GMAQR  GLTLASLIQG
Sbjct: 393 RDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQG 452

Query: 121 FDWERVSTSLVDMTE 136
           F+WER++ SLVDM+E
Sbjct: 453 FEWERLNNSLVDMSE 467

[Close]

BLAST of Sed0017335 vs. NCBI nr
Match: XP_022943757.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 227.3 bits (578), Expect = 8.6e-56
Identity = 103/135 (76.30%), Postives = 119/135 (88.15%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           +VNESDL SLSY+QGII+ETLR+   APLL+PHCA E+CKIEGYDVPRDT++ IN WA+H
Sbjct: 332 LVNESDLPSLSYVQGIISETLRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMH 391

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDPNLWEDATSFKPERH      G+E+YKLIPFGLGRRACPG+GMAQR  GLTLASLIQG
Sbjct: 392 RDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQG 451

Query: 121 FDWERVSTSLVDMTE 136
           F+WER++ SLVDM+E
Sbjct: 452 FEWERLNNSLVDMSE 466

[Close]

BLAST of Sed0017335 vs. NCBI nr
Match: XP_022943410.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata] >KAG7011036.1 Isoflavone 2'-hydroxylase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 227.3 bits (578), Expect = 8.6e-56
Identity = 103/135 (76.30%), Postives = 119/135 (88.15%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           +VNESDL SLSY+QGII+ETLR+   APLL+PHCA E+CKIEGYDVPRDT++ IN WA+H
Sbjct: 333 LVNESDLPSLSYVQGIISETLRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMH 392

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDPNLWEDATSFKPERH      G+E+YKLIPFGLGRRACPG+GMAQR  GLTLASLIQG
Sbjct: 393 RDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQG 452

Query: 121 FDWERVSTSLVDMTE 136
           F+WER++ SLVDM+E
Sbjct: 453 FEWERLNNSLVDMSE 467

[Close]

BLAST of Sed0017335 vs. NCBI nr
Match: XP_022149455.1 (cytochrome P450 81E8-like [Momordica charantia])

HSP 1 Score: 226.5 bits (576), Expect = 1.5e-55
Identity = 107/135 (79.26%), Postives = 122/135 (90.37%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           +VNE+DLSSL YLQGII ETLR++  APLL+PHCAS DCKI GYDVPRDT++LIN+WAIH
Sbjct: 333 LVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIH 392

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDPNLWEDAT+FKPERH GN  IG ++YKL+PFG GRR+CPGVGMAQR VGLTLASLIQG
Sbjct: 393 RDPNLWEDATNFKPERH-GN-TIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQG 452

Query: 121 FDWERVSTSLVDMTE 136
           F+WER+S SLVDMTE
Sbjct: 453 FEWERMSNSLVDMTE 465

[Close]

BLAST of Sed0017335 vs. NCBI nr
Match: XP_023512186.1 (isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512187.1 isoflavone 2'-hydroxylase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 226.5 bits (576), Expect = 1.5e-55
Identity = 102/135 (75.56%), Postives = 120/135 (88.89%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           +VNE DL +LSY+QGII+ET+R++  APLLVPHCA E+CKIEGYDVPRDT++LIN WA+H
Sbjct: 332 LVNEVDLPNLSYVQGIISETMRLNPAAPLLVPHCAFEECKIEGYDVPRDTIILINAWAMH 391

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDPNLWEDATSFKPERH      G+++YKLIPFGLGRRACPGVGMAQR  GLTLASLIQG
Sbjct: 392 RDPNLWEDATSFKPERHANGMKNGADSYKLIPFGLGRRACPGVGMAQRVAGLTLASLIQG 451

Query: 121 FDWERVSTSLVDMTE 136
           F+WER++ SLVDM+E
Sbjct: 452 FEWERLNNSLVDMSE 466

[Close]

BLAST of Sed0017335 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.1e-42
Identity = 75/135 (55.56%), Postives = 102/135 (75.56%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           ++ E DL  L+YL  II+ET R+   AP+LVPH +S+DCK++GYDVP+ T++L+N WAIH
Sbjct: 338 LIEEQDLPKLTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIH 397

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDP  W++ T FKPERH   G +  E  KL+PFG+GRR+CPG G+AQR VGLTL +LIQ 
Sbjct: 398 RDPEFWDEPTLFKPERH---GGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQC 457

Query: 121 FDWERVSTSLVDMTE 136
           F+W+R+  + +DM E
Sbjct: 458 FEWKRIGEAKIDMAE 469

[Close]

BLAST of Sed0017335 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 2.5e-42
Identity = 83/135 (61.48%), Postives = 96/135 (71.11%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           ++ E+DLS L YL+ I+ ETLR+    PLLVPH ASEDCKI  YD+PR T +L+N WAIH
Sbjct: 345 LIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIH 404

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDPN W+D  SFKPER         EA KL+ FGLGRRACPG G+AQR VGL L SLIQ 
Sbjct: 405 RDPNTWDDPDSFKPERF----EKEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQC 464

Query: 121 FDWERVSTSLVDMTE 136
           F+WERV    VDM E
Sbjct: 465 FEWERVGNVEVDMKE 475

[Close]

BLAST of Sed0017335 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 2.8e-41
Identity = 78/135 (57.78%), Postives = 98/135 (72.59%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           +V E D+  L YLQ I++ETLR+  VAP+L+PH ASEDC ++GYDVPR T++L+N WAIH
Sbjct: 347 LVEEQDIVKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIH 406

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDP LWE+   FKPER    G    E  KL+PFG+GRR+CPG G+AQR V L L SL+Q 
Sbjct: 407 RDPKLWEEPEKFKPERFEKKG----EDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQC 466

Query: 121 FDWERVSTSLVDMTE 136
           F+WERV    +DM E
Sbjct: 467 FEWERVEEKYLDMRE 477

[Close]

BLAST of Sed0017335 vs. ExPASy Swiss-Prot
Match: Q0WTF4 (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 2.4e-40
Identity = 74/137 (54.01%), Postives = 98/137 (71.53%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           +++E D+++L YLQ I++ET R+   APLLVP   +ED K+ GYDVPR TMV++N WAIH
Sbjct: 334 LIDEPDIANLPYLQNIVSETFRLYPAAPLLVPRSPTEDIKVGGYDVPRGTMVMVNAWAIH 393

Query: 61  RDPNLWEDATSFKPERHIG--NGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLI 120
           RDP LW +   FKPER  G   G  G + +KL+PFG GRR+CPG G+ Q+ V L L SLI
Sbjct: 394 RDPELWNEPEKFKPERFNGGEGGGRGEDVHKLMPFGNGRRSCPGAGLGQKIVTLALGSLI 453

Query: 121 QGFDWERVSTSLVDMTE 136
           Q FDW++V+   +DMTE
Sbjct: 454 QCFDWQKVNGEAIDMTE 470

[Close]

BLAST of Sed0017335 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 5.8e-39
Identity = 74/135 (54.81%), Postives = 99/135 (73.33%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           +V+ESDL  L+YL+ +I ETLR+   APLL+PH  +++C + GY VPRDT+VLIN WAIH
Sbjct: 341 LVDESDLPKLTYLRNVIYETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIH 400

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDP  W +AT+FKPER    G    E  K+I FG+GRRACPG G+A R + +TLA L+Q 
Sbjct: 401 RDPETWSEATTFKPERFDKKG----ELEKMIAFGMGRRACPGEGLALRAISMTLALLVQC 460

Query: 121 FDWERVSTSLVDMTE 136
           FDW+R++   +DM+E
Sbjct: 461 FDWKRINDEKIDMSE 471

[Close]

BLAST of Sed0017335 vs. ExPASy TrEMBL
Match: A0A6J1JDS1 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111483622 PE=3 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 6.4e-57
Identity = 104/135 (77.04%), Postives = 121/135 (89.63%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           +VNE+DL SLSY+QGII+ETLR+   APLL+PHCASE+CKIEGYDVPRDT++LIN WA+H
Sbjct: 333 LVNEADLPSLSYVQGIISETLRLHPAAPLLLPHCASEECKIEGYDVPRDTIILINAWAMH 392

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDPNLWEDATSFKPERH      G+E+YKLIPFGLGRRACPG+GMAQR  GLTLASLIQG
Sbjct: 393 RDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQG 452

Query: 121 FDWERVSTSLVDMTE 136
           F+WER++ SLVDM+E
Sbjct: 453 FEWERLNNSLVDMSE 467

[Close]

BLAST of Sed0017335 vs. ExPASy TrEMBL
Match: A0A6J1FXS5 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448183 PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 4.2e-56
Identity = 103/135 (76.30%), Postives = 119/135 (88.15%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           +VNESDL SLSY+QGII+ETLR+   APLL+PHCA E+CKIEGYDVPRDT++ IN WA+H
Sbjct: 333 LVNESDLPSLSYVQGIISETLRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMH 392

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDPNLWEDATSFKPERH      G+E+YKLIPFGLGRRACPG+GMAQR  GLTLASLIQG
Sbjct: 393 RDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQG 452

Query: 121 FDWERVSTSLVDMTE 136
           F+WER++ SLVDM+E
Sbjct: 453 FEWERLNNSLVDMSE 467

[Close]

BLAST of Sed0017335 vs. ExPASy TrEMBL
Match: A0A6J1FTX9 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448413 PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 4.2e-56
Identity = 103/135 (76.30%), Postives = 119/135 (88.15%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           +VNESDL SLSY+QGII+ETLR+   APLL+PHCA E+CKIEGYDVPRDT++ IN WA+H
Sbjct: 332 LVNESDLPSLSYVQGIISETLRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMH 391

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDPNLWEDATSFKPERH      G+E+YKLIPFGLGRRACPG+GMAQR  GLTLASLIQG
Sbjct: 392 RDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQG 451

Query: 121 FDWERVSTSLVDMTE 136
           F+WER++ SLVDM+E
Sbjct: 452 FEWERLNNSLVDMSE 466

[Close]

BLAST of Sed0017335 vs. ExPASy TrEMBL
Match: A0A6J1D5S1 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 7.1e-56
Identity = 107/135 (79.26%), Postives = 122/135 (90.37%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           +VNE+DLSSL YLQGII ETLR++  APLL+PHCAS DCKI GYDVPRDT++LIN+WAIH
Sbjct: 333 LVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIH 392

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDPNLWEDAT+FKPERH GN  IG ++YKL+PFG GRR+CPGVGMAQR VGLTLASLIQG
Sbjct: 393 RDPNLWEDATNFKPERH-GN-TIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQG 452

Query: 121 FDWERVSTSLVDMTE 136
           F+WER+S SLVDMTE
Sbjct: 453 FEWERMSNSLVDMTE 465

[Close]

BLAST of Sed0017335 vs. ExPASy TrEMBL
Match: A0A6J1G5P7 (cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 1.2e-55
Identity = 105/135 (77.78%), Postives = 120/135 (88.89%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           +VNESDL +L YLQGII+ETLR++  APLLVPHCA +DCKIEGYDVPRDT+VLIN WAIH
Sbjct: 333 LVNESDLPNLKYLQGIISETLRLNPAAPLLVPHCAFQDCKIEGYDVPRDTIVLINTWAIH 392

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDPNLWED T+FKPERH      G+++YKL+PFGLGRRACPGVGMAQR VGL LASLIQG
Sbjct: 393 RDPNLWEDPTAFKPERH--GDTSGADSYKLLPFGLGRRACPGVGMAQRVVGLALASLIQG 452

Query: 121 FDWERVSTSLVDMTE 136
           F+WERVS+SLVDM+E
Sbjct: 453 FEWERVSSSLVDMSE 465

[Close]

BLAST of Sed0017335 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 178.3 bits (451), Expect = 4.3e-45
Identity = 84/135 (62.22%), Postives = 103/135 (76.30%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           ++ ESD+ +L YLQ I++ETLR+   AP+L+PH AS+DCK+ GYD+PR TM+L N WAIH
Sbjct: 339 LLEESDIPNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIH 398

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDP LW+D TSFKPER    G    EA KL+PFGLGRRACPG G+AQR V L+L SLIQ 
Sbjct: 399 RDPLLWDDPTSFKPERFEKEG----EAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQC 458

Query: 121 FDWERVSTSLVDMTE 136
           F+WER+    VDMTE
Sbjct: 459 FEWERIGEEEVDMTE 469

[Close]

BLAST of Sed0017335 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 177.2 bits (448), Expect = 9.5e-45
Identity = 84/135 (62.22%), Postives = 100/135 (74.07%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           +V ESDLS L YLQ I++E+LR+   +PLLVPH ASEDCK+ GY +PR TM+L N WAIH
Sbjct: 339 LVEESDLSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIH 398

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDP +W+D TSFKPER    G    EA KL+ FGLGRRACPG G+AQR   LT+ SLIQ 
Sbjct: 399 RDPKIWDDPTSFKPERFEKEG----EAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQC 458

Query: 121 FDWERVSTSLVDMTE 136
           F+WER+    VDMTE
Sbjct: 459 FEWERIGEEEVDMTE 469

[Close]

BLAST of Sed0017335 vs. TAIR 10
Match: AT1G66540.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 174.9 bits (442), Expect = 4.7e-44
Identity = 81/135 (60.00%), Postives = 101/135 (74.81%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           ++ ESD+ +L YLQ I++ETLR+    PLLVPH +SEDCK+ GYD+P  TM+L+NVWAIH
Sbjct: 226 LLEESDIPNLPYLQNIVSETLRLYPAGPLLVPHISSEDCKVGGYDMPCGTMLLVNVWAIH 285

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDP LW+D  SFKPER    G    E +KL+ FGLGRRACPG G+A+R V L+L SLIQ 
Sbjct: 286 RDPRLWDDPASFKPERFEKEG----ETHKLLTFGLGRRACPGSGLARRLVSLSLGSLIQC 345

Query: 121 FDWERVSTSLVDMTE 136
           F+WER+    VDMTE
Sbjct: 346 FEWERIGEEEVDMTE 356

[Close]

BLAST of Sed0017335 vs. TAIR 10
Match: AT1G66540.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 174.9 bits (442), Expect = 4.7e-44
Identity = 81/135 (60.00%), Postives = 101/135 (74.81%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           ++ ESD+ +L YLQ I++ETLR+    PLLVPH +SEDCK+ GYD+P  TM+L+NVWAIH
Sbjct: 167 LLEESDIPNLPYLQNIVSETLRLYPAGPLLVPHISSEDCKVGGYDMPCGTMLLVNVWAIH 226

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDP LW+D  SFKPER    G    E +KL+ FGLGRRACPG G+A+R V L+L SLIQ 
Sbjct: 227 RDPRLWDDPASFKPERFEKEG----ETHKLLTFGLGRRACPGSGLARRLVSLSLGSLIQC 286

Query: 121 FDWERVSTSLVDMTE 136
           F+WER+    VDMTE
Sbjct: 287 FEWERIGEEEVDMTE 297

[Close]

BLAST of Sed0017335 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 173.7 bits (439), Expect = 1.0e-43
Identity = 81/135 (60.00%), Postives = 103/135 (76.30%), Query Frame = 0


Query: 1   MVNESDLSSLSYLQGIIAETLRISLVAPLLVPHCASEDCKIEGYDVPRDTMVLINVWAIH 60
           +++ESD+S+L YLQ I++ETLR+   AP+L+PH ASEDCK+ GYD+PR T++L NVWAIH
Sbjct: 338 LMDESDISNLPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIH 397

Query: 61  RDPNLWEDATSFKPERHIGNGNIGSEAYKLIPFGLGRRACPGVGMAQRFVGLTLASLIQG 120
           RDP LW+D  SFKPER    G    EA KL+PFGLGRRACPG G+A R + LTL SLIQ 
Sbjct: 398 RDPQLWDDPMSFKPERFEKEG----EAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQC 457

Query: 121 FDWERVSTSLVDMTE 136
            +WE++    VDM+E
Sbjct: 458 LEWEKIGEE-VDMSE 467

The following BLAST results are available for this feature:

BLAST of Sed0017335 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022985609.1	1.3e-56	77.04	isoflavone 2'-hydroxylase-like [Cucurbita maxima]	[more]
XP_022943757.1	8.6e-56	76.30	isoflavone 2'-hydroxylase-like [Cucurbita moschata]	[more]
XP_022943410.1	8.6e-56	76.30	isoflavone 2'-hydroxylase-like [Cucurbita moschata] >KAG7011036.1 Isoflavone 2'-...	[more]
XP_022149455.1	1.5e-55	79.26	cytochrome P450 81E8-like [Momordica charantia]	[more]
XP_023512186.1	1.5e-55	75.56	isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_02351...	[more]

BLAST of Sed0017335 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	1.1e-42	55.56	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q9FG65	2.5e-42	61.48	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]
Q9LHA1	2.8e-41	57.78	Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1	[more]
Q0WTF4	2.4e-40	54.01	Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1	[more]
Q6WNR0	5.8e-39	54.81	Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1	[more]

BLAST of Sed0017335 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JDS1	6.4e-57	77.04	isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111483622 PE=3 ...	[more]
A0A6J1FXS5	4.2e-56	76.30	isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448183 PE=...	[more]
A0A6J1FTX9	4.2e-56	76.30	isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448413 PE=...	[more]
A0A6J1D5S1	7.1e-56	79.26	cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV...	[more]
A0A6J1G5P7	1.2e-55	77.78	cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1	[more]

BLAST of Sed0017335 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37360.1	4.3e-45	62.22	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT4G37340.1	9.5e-45	62.22	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]
AT1G66540.1	4.7e-44	60.00	Cytochrome P450 superfamily protein	[more]
AT1G66540.2	4.7e-44	60.00	Cytochrome P450 superfamily protein	[more]
AT4G37370.1	1.0e-43	60.00	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 90..100 score: 55.18 coord: 100..123 score: 33.68 coord: 14..32 score: 34.91 coord: 55..79 score: 30.91
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 4..135 e-value: 7.9E-40 score: 137.0
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..142 e-value: 4.7E-46 score: 159.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 2..139
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 1..139
None	No IPR available	PANTHER	PTHR24298:SF836	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATED	coord: 1..139
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 93..102

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0017335.1	Sed0017335.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen