Sed0016701 (gene) Chayote v1

Overview
NameSed0016701
Typegene
OrganismSechium edule (Chayote v1)
Description3-oxo-Delta(4,5)-steroid 5-beta-reductase-like
LocationLG01: 8633229 .. 8635437 (-)
RNA-Seq ExpressionSed0016701
SyntenySed0016701
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCCGAAACAATCAACCCAAAATTAGTCGCCGTCATTTTCGGAGTCACCGGCATGGTGGGAAAAGAGTTGGCCAAAACCCTTCTCGGCTCAACCGCCGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGACAACGTCAGCCCCATCGCCGATCCCAACTTCCACTTCATCGCTTGCGATTTGCTCGACCGCCACGTGGCTCACCAAAACCTCTCGCAAATCCGCCACGTCACGCACTTGTTTTGGGTCACTTGGGCCGCCCAGCTCCGGTTCGATTCTCAAGATTGCAACGACCAAAATCGGGCCATGTTGGCCAACGCACTCGACGCGGTTCTCCCAACGGCGCCGGATCTCCGCCACGTGTCTCTCCAGACCGGGATCAAGCACTACGTTTCGTTGACCAGCGCCGCCGTGACTGGCGGCGGAAGAGGGGAGGTGGTTTATTGCGAGGAGAGTCCGAGAGCGAATTCAGGGAGTAATTTTTACTATGCGTTGGAGGATCTCCTCCGTGAGCGGCTTTCGGGCGGCGGCGGCGGCGGAAAAAGGGTGGCGTGGAGTGTACTCCGGCCCGGTTTGATTTTGGGTTGTTCGAACCGGACTTTTTTCAATTTTATGGGGAGTTTGTGTGTTTATGGGGCGATTTGTAAGAAGCTGAAGAAGCCGTTTGTGTTTGGCGGCACGGCGGCGTGCTGGTCGGAGGTTTGTATTGACGGCTCCGACGCTCGGCTTGTGGCGGAGCAGCACGTTTGGGCGGCAACCAAACAAATAAATGGTACGTGTGACTTGTCATATTTATTTTTTCGAAATAAGTAGATTTTTTTTTTCTTTTCTTTGTTAGGTCAAACTCAGGACAACACGAGGCTGATATGATAAGAGTATTTTCTAAATTGAATCGGTATATTTAAATTACAATACAATTAATCAAACTCGCCAACATATTACCAACTCACTTACGGAAATCAACTTAAAAAAAAAAAATGGAAATTAGCCAGTTCATACAGTTTTTTTTTTTTTTTTTATATTTTTATTTTCTAATTTGATTTTGATAAACTATTTAACATACATAATCAACAAAAAAAAAACATCATTTTATATTTATGTATTTATTTCGAACTATAATAGTTCAACGATATGAGTGTGTCTTTTAATTAAAATGTCTTGAGTTTGAAATCCTTCCTTCTCTAAATATAATTGAGCTAAATTAAAGTTGATTTCATATTTTGTTTTTGAATTTTACAACAATTGGGAGTTTGTCATATCATTAAGTTATTACTCTTAATGTTGAATTCCAGGTACTTATATCAACACCAAATTAAAAATAATAACTATTTGTTAGGTTTTATGGAATGTTAGGAATTAAATTCTAGATTTTACGATCATGAATTAAATTTTATCTTTTTCTAAAAAATGTGGAACACATTTGTAATTTAACATAATATTTATTTTATAAAAGTTAAAATACCATTTTGATCTATATATTTTAGAGTTTGTTTCATATATTTTCAAAAAGAGTAATTTACTTTATTTCTTGATATTATCTATATTTGAAGCATAAACATATGACCTAATTTGAGTTACAATTTATTTTTCTTTAACATAAGTAAGTATTAATGTATCAAAATTTAACATATAAGTAAGTACGAAAGCTATAGAGCAATAAATGTATCCGTGTGAATCAAAAGGCTACAGATTTTATATTTATCCGTTGTTGGACTAAAAAAGAAATATAGAAATTAAAATACTATTATTTTTGCTTTTAAAAAAAATAAAAAATGATAATTTTAATAAAATATAGATGTGATATCTGTTATGTGAAACAGGCAGCGGAGAGGGGGAAGCATTCAACGCGTGTAACGGGTGGAGCTTCAGGTGGAAGGAGATATGGGGAGCGGTGGCGGAGAAACTAGGAGTGGCGGCGGCGGCGGCGGAGATGTTTTCGCCGGAGTTCAACTACACGGCGGCGATGGGGGACAAAGGGGAGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGTTTCCGACGGAAATGGAGGAGCTGGCCAATTGGGGGCTCATGGACAGCTTGTTTAGGCTGCCGGGAAAAATGGTGGCCAGTCGCCACAAATCCGACCGTTTTGGTTTCACCCTAAAATACAAAATGTTGGACTCCATCTTCTATTGGATTGATCTCATGAGACGTGACAATTTGATCCCTTCTTCTTAA

mRNA sequence

ATGGCGGCCGAAACAATCAACCCAAAATTAGTCGCCGTCATTTTCGGAGTCACCGGCATGGTGGGAAAAGAGTTGGCCAAAACCCTTCTCGGCTCAACCGCCGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGACAACGTCAGCCCCATCGCCGATCCCAACTTCCACTTCATCGCTTGCGATTTGCTCGACCGCCACGTGGCTCACCAAAACCTCTCGCAAATCCGCCACGTCACGCACTTGTTTTGGGTCACTTGGGCCGCCCAGCTCCGGTTCGATTCTCAAGATTGCAACGACCAAAATCGGGCCATGTTGGCCAACGCACTCGACGCGGTTCTCCCAACGGCGCCGGATCTCCGCCACGTGTCTCTCCAGACCGGGATCAAGCACTACGTTTCGTTGACCAGCGCCGCCGTGACTGGCGGCGGAAGAGGGGAGGTGGTTTATTGCGAGGAGAGTCCGAGAGCGAATTCAGGGAGTAATTTTTACTATGCGTTGGAGGATCTCCTCCGTGAGCGGCTTTCGGGCGGCGGCGGCGGCGGAAAAAGGGTGGCGTGGAGTGTACTCCGGCCCGGTTTGATTTTGGGTTGTTCGAACCGGACTTTTTTCAATTTTATGGGGAGTTTGTGTGTTTATGGGGCGATTTGTAAGAAGCTGAAGAAGCCGTTTGTGTTTGGCGGCACGGCGGCGTGCTGGTCGGAGGTTTGTATTGACGGCTCCGACGCTCGGCTTGTGGCGGAGCAGCACGTTTGGGCGGCAACCAAACAAATAAATGGCAGCGGAGAGGGGGAAGCATTCAACGCGTGTAACGGGTGGAGCTTCAGGTGGAAGGAGATATGGGGAGCGGTGGCGGAGAAACTAGGAGTGGCGGCGGCGGCGGCGGAGATGTTTTCGCCGGAGTTCAACTACACGGCGGCGATGGGGGACAAAGGGGAGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGTTTCCGACGGAAATGGAGGAGCTGGCCAATTGGGGGCTCATGGACAGCTTGTTTAGGCTGCCGGGAAAAATGGTGGCCAGTCGCCACAAATCCGACCGTTTTGGTTTCACCCTAAAATACAAAATGTTGGACTCCATCTTCTATTGGATTGATCTCATGAGACGTGACAATTTGATCCCTTCTTCTTAA

Coding sequence (CDS)

ATGGCGGCCGAAACAATCAACCCAAAATTAGTCGCCGTCATTTTCGGAGTCACCGGCATGGTGGGAAAAGAGTTGGCCAAAACCCTTCTCGGCTCAACCGCCGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGACAACGTCAGCCCCATCGCCGATCCCAACTTCCACTTCATCGCTTGCGATTTGCTCGACCGCCACGTGGCTCACCAAAACCTCTCGCAAATCCGCCACGTCACGCACTTGTTTTGGGTCACTTGGGCCGCCCAGCTCCGGTTCGATTCTCAAGATTGCAACGACCAAAATCGGGCCATGTTGGCCAACGCACTCGACGCGGTTCTCCCAACGGCGCCGGATCTCCGCCACGTGTCTCTCCAGACCGGGATCAAGCACTACGTTTCGTTGACCAGCGCCGCCGTGACTGGCGGCGGAAGAGGGGAGGTGGTTTATTGCGAGGAGAGTCCGAGAGCGAATTCAGGGAGTAATTTTTACTATGCGTTGGAGGATCTCCTCCGTGAGCGGCTTTCGGGCGGCGGCGGCGGCGGAAAAAGGGTGGCGTGGAGTGTACTCCGGCCCGGTTTGATTTTGGGTTGTTCGAACCGGACTTTTTTCAATTTTATGGGGAGTTTGTGTGTTTATGGGGCGATTTGTAAGAAGCTGAAGAAGCCGTTTGTGTTTGGCGGCACGGCGGCGTGCTGGTCGGAGGTTTGTATTGACGGCTCCGACGCTCGGCTTGTGGCGGAGCAGCACGTTTGGGCGGCAACCAAACAAATAAATGGCAGCGGAGAGGGGGAAGCATTCAACGCGTGTAACGGGTGGAGCTTCAGGTGGAAGGAGATATGGGGAGCGGTGGCGGAGAAACTAGGAGTGGCGGCGGCGGCGGCGGAGATGTTTTCGCCGGAGTTCAACTACACGGCGGCGATGGGGGACAAAGGGGAGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGTTTCCGACGGAAATGGAGGAGCTGGCCAATTGGGGGCTCATGGACAGCTTGTTTAGGCTGCCGGGAAAAATGGTGGCCAGTCGCCACAAATCCGACCGTTTTGGTTTCACCCTAAAATACAAAATGTTGGACTCCATCTTCTATTGGATTGATCTCATGAGACGTGACAATTTGATCCCTTCTTCTTAA

Protein sequence

MAAETINPKLVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADPNFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDARLVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEMFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIPSS
Homology
BLAST of Sed0016701 vs. NCBI nr
Match: XP_022998181.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima])

HSP 1 Score: 648.3 bits (1671), Expect = 4.2e-182
Identity = 319/395 (80.76%), Postives = 344/395 (87.09%), Query Frame = 0

Query: 1   MAAETINPK------LVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
           MAAETIN K      LVAVIFGVTG+VGKELAKTLL ST GWKVYGVARRPDNVSPI D 
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKTLL-STGGWKVYGVARRPDNVSPITDT 60

Query: 61  NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
           NF FI+CDLLDR  AH+NLS +RHVTHLFWVTW AQLRFD+ DC+DQNR MLAN LD +L
Sbjct: 61  NFQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWTAQLRFDTSDCSDQNRTMLANVLDVIL 120

Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEV-VYCEESPRANSGSNFYYALEDLLRE 180
           P AP LRHVSLQTGIKHYVSLT AAV GGG G + VYCE+SPRA SG+NFYYALEDLLRE
Sbjct: 121 PAAPALRHVSLQTGIKHYVSLTGAAVDGGGGGGMAVYCEDSPRAESGNNFYYALEDLLRE 180

Query: 181 RLSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACW 240
           RLS   GGG+RVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGG+AACW
Sbjct: 181 RLS---GGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACW 240

Query: 241 SEVCIDGSDARLVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAA 300
              CI+GSDARLVAEQHVWAAT +IN + +GEAFN CNGWSF WKEIWGAVAEK G+AAA
Sbjct: 241 EGFCINGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGLAAA 300

Query: 301 AAE----MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMV 360
           AAE    MFSPEF+YTAAM +KG+VWAEIVEEEGL PTEMEELANWG +DSLFRLPGKMV
Sbjct: 301 AAEDEKGMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMV 360

Query: 361 ASRHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIP 385
           ASR+KSDR GF++KYKMLDSI YWID+MR D LIP
Sbjct: 361 ASRNKSDRLGFSVKYKMLDSILYWIDVMRYDTLIP 391

BLAST of Sed0016701 vs. NCBI nr
Match: XP_022948773.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata])

HSP 1 Score: 643.7 bits (1659), Expect = 1.0e-180
Identity = 315/393 (80.15%), Postives = 340/393 (86.51%), Query Frame = 0

Query: 1   MAAETINPK------LVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
           MAAETIN K      LVAVIFGVTG+VGKELAK LL ST GWKVYGVARRPDNVSPI D 
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALL-STGGWKVYGVARRPDNVSPITDT 60

Query: 61  NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
           NF FI+CDLLDR  AH+NLS +RHVTH+FWVTWAAQLRFD+ DC+DQNR MLAN LD +L
Sbjct: 61  NFQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVIL 120

Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRER 180
           P AP LRHVSLQTGIKHYVSLT AAV GGG    VYCE+SPRA SG+NFYYALEDLLRER
Sbjct: 121 PAAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRER 180

Query: 181 LSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWS 240
           LS   GGG+RVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGG+AACW 
Sbjct: 181 LS---GGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWE 240

Query: 241 EVCIDGSDARLVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAA 300
             CI+GSDARLVAEQHVWAAT +IN + +GEAFN CNGWSF WKEIWGAVAEK GVAAAA
Sbjct: 241 GFCINGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAA 300

Query: 301 AE---MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVAS 360
            +   MFSPEF+YTAAM +KG +WAEIVEEEGL PTEMEELANWG +DSLFRLPGKMVAS
Sbjct: 301 EDEEGMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVAS 360

Query: 361 RHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIP 385
           R+KSDR GF++KYKMLDSI YWID+MR D LIP
Sbjct: 361 RNKSDRLGFSVKYKMLDSILYWIDVMRYDKLIP 389

BLAST of Sed0016701 vs. NCBI nr
Match: KAG6607304.1 ((S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 641.7 bits (1654), Expect = 4.0e-180
Identity = 315/393 (80.15%), Postives = 339/393 (86.26%), Query Frame = 0

Query: 1   MAAETINPK------LVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
           M AETIN K      LVAVIFGVTG+VGKELAK LL ST GWKVYGVARRPDNVSPI D 
Sbjct: 1   MTAETINAKLDGNGELVAVIFGVTGLVGKELAKALL-STGGWKVYGVARRPDNVSPITDT 60

Query: 61  NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
           NF FI+CDLLDR  A++NLS +RHVTHLFWVTWAAQLRFD+ DC+DQNR MLAN LD +L
Sbjct: 61  NFQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVIL 120

Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRER 180
           P AP LRHVSLQTGIKHYVSLT AAV GGG    VYCE+SPRA SG+NFYYALEDLLRER
Sbjct: 121 PAAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRER 180

Query: 181 LSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWS 240
           LS   GGG+RVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGG+AACW 
Sbjct: 181 LS---GGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWE 240

Query: 241 EVCIDGSDARLVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAA 300
             CI+GSDARLVAEQHVWAAT +IN + +GEAFN CNGWSF WKEIWGAVAEK GVAAAA
Sbjct: 241 GFCINGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAA 300

Query: 301 AE---MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVAS 360
            +   MFSPEF+YTAAM +KG VWAEIVEEEGL PTEMEELANWG +DSLFRLPGKMVAS
Sbjct: 301 EDEEGMFSPEFHYTAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVAS 360

Query: 361 RHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIP 385
           R+KSDR GF++KYKMLDSI YWID+MR D LIP
Sbjct: 361 RNKSDRLGFSVKYKMLDSILYWIDVMRYDKLIP 389

BLAST of Sed0016701 vs. NCBI nr
Match: KAG7036983.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 641.0 bits (1652), Expect = 6.8e-180
Identity = 315/393 (80.15%), Postives = 339/393 (86.26%), Query Frame = 0

Query: 1   MAAETINPK------LVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
           MAAETIN K      LVAVIFGVTG+VGKELAK LL ST GWKVYGVARRPDNVSPI D 
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALL-STGGWKVYGVARRPDNVSPITDT 60

Query: 61  NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
           NF FI+CDLLDR  A++NLS +RHVTHLFWVTWAAQLRFD+ DC+DQNR MLAN LD +L
Sbjct: 61  NFQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVIL 120

Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRER 180
           P AP LRHVSLQTGIKHYVSLT AAV GGG    VYCE+SPRA SG+NFYYALEDLLRER
Sbjct: 121 PAAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRER 180

Query: 181 LSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWS 240
           LS   GGG+RVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGG+AACW 
Sbjct: 181 LS---GGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWE 240

Query: 241 EVCIDGSDARLVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAA 300
             CI+GSDARLVAEQHVWAAT +IN + +GEAFN CNGWSF WKEIWGAVAEK GVAAAA
Sbjct: 241 GFCINGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAA 300

Query: 301 AE---MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVAS 360
            +   MFSPEF+Y AAM +KG VWAEIVEEEGL PTEMEELANWG +DSLFRLPGKMVAS
Sbjct: 301 EDEEGMFSPEFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVAS 360

Query: 361 RHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIP 385
           R+KSDR GF++KYKMLDSI YWID+MR D LIP
Sbjct: 361 RNKSDRLGFSVKYKMLDSILYWIDVMRYDKLIP 389

BLAST of Sed0016701 vs. NCBI nr
Match: XP_038895390.1 ((S)-8-oxocitronellyl enol synthase CYC2 [Benincasa hispida])

HSP 1 Score: 639.0 bits (1647), Expect = 2.6e-179
Identity = 318/397 (80.10%), Postives = 341/397 (85.89%), Query Frame = 0

Query: 1   MAAETI------NPKLVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
           MAAE I      N +LVAVIFGVTG+VGKELAKTLL STAGWKVYGVARRPDN+SPI+ P
Sbjct: 1   MAAEIINTNLVANAELVAVIFGVTGLVGKELAKTLL-STAGWKVYGVARRPDNISPISHP 60

Query: 61  NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
           NFHFI+CDLLDR  AH NLS +RHVTHLFW+TWAAQLRFDSQ C+DQNRAMLANALDA+L
Sbjct: 61  NFHFISCDLLDRRSAHSNLSPVRHVTHLFWITWAAQLRFDSQGCSDQNRAMLANALDAIL 120

Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRER 180
           P+AP LRHVSLQTGIKHY SLT  A   GG GEV YCE+SPRA SG+NFYY LEDLLRER
Sbjct: 121 PSAPALRHVSLQTGIKHYASLTRLAAGCGGGGEVAYCEDSPRAESGNNFYYVLEDLLRER 180

Query: 181 LSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWS 240
           LS   GG + V WSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLK PFVFGGTAACW 
Sbjct: 181 LS---GGRRVVGWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWE 240

Query: 241 EVCIDGSDARLVAEQHVWAATK--QINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAA 300
           EV IDGSDARL AEQHVWAATK  +IN + +GEAFN CNGWSFRWKEIWGAVAEKLGVAA
Sbjct: 241 EVYIDGSDARLTAEQHVWAATKGIKINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVAA 300

Query: 301 AAAE----MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKM 360
           A  E    MFS EF+YTAAMGDKG++WAEIVEEEGL PTEMEELANWG +D+LFRLP KM
Sbjct: 301 AEKEDERIMFSAEFHYTAAMGDKGKLWAEIVEEEGLVPTEMEELANWGFLDTLFRLPEKM 360

Query: 361 VASRHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIPS 386
           VASR KSDRFGFT+KYKMLDSI YWID+MR D LIPS
Sbjct: 361 VASRAKSDRFGFTVKYKMLDSILYWIDVMRNDKLIPS 393

BLAST of Sed0016701 vs. ExPASy Swiss-Prot
Match: A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 6.4e-64
Identity = 154/381 (40.42%), Postives = 208/381 (54.59%), Query Frame = 0

Query: 11  VAVIFGVTGMVGKELAKTL-LGSTAG--WKVYGVARRPDNVSPIADPNFHFIACDLLDRH 70
           VA+I GVTG+VG  LA+ L +  T G  WKVYGVARRP   +  AD    +I CD+ D  
Sbjct: 28  VALIIGVTGIVGNSLAEILPISDTPGGPWKVYGVARRP-RPAWNADHPVEYIQCDISDAS 87

Query: 71  VAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQT 130
             H  LS +  VTH+FWVTWA   R    +C + N  ML N L+A++P A +L H+ LQT
Sbjct: 88  DTHTKLSPLTDVTHIFWVTWAN--RPTESECCELNGTMLRNVLNALIPKAANLHHICLQT 147

Query: 131 GIKHYVSLTSAAVTGGGR---GEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKR 190
           G KHY+    A     G+    E  + E+ PR N+  NFYY LED+L E      G    
Sbjct: 148 GHKHYIGPFEAF----GKIKPHEPPFTEDMPRLNA-PNFYYTLEDMLVEASEKKAG---- 207

Query: 191 VAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDAR 250
           + WSV RP +I G S  +  N +G+LCVY AICK    P  F GT A W+   +  SDA 
Sbjct: 208 LNWSVHRPAVIFGFSPFSMMNIIGTLCVYAAICKHENTPLKFPGTKAAWNCYSV-ASDAD 267

Query: 251 LVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEMFSPEFNY 310
           L+AE  +WAA   ++   + EAFN  NG  F+WK +W  +AE+ GV  A  +      + 
Sbjct: 268 LIAEHQIWAA---VDPYAKNEAFNCSNGDLFKWKHLWKVLAEQFGVEYAEFDESEKPTSL 327

Query: 311 TAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGK-MVASRHKSDRFGFTLK 370
              M DKG VW EIV E GL  T++E +A W   D +  L G+ ++ S +KS   G+   
Sbjct: 328 VERMKDKGPVWEEIVRENGLHTTKLEGVATWWFADVI--LGGECLLDSMNKSKEHGYLGF 387

Query: 371 YKMLDSIFYWIDLMRRDNLIP 385
               +S+   ID M+   ++P
Sbjct: 388 RNTKNSLISVIDKMKAHKIVP 390

BLAST of Sed0016701 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.5e-60
Identity = 146/377 (38.73%), Postives = 202/377 (53.58%), Query Frame = 0

Query: 11  VAVIFGVTGMVGKELAKTL-LGSTAG--WKVYGVARRPDNVSPIADPNFHFIACDLLDRH 70
           VA+I GVTG+VG  LA+ L L  T G  WKVYGVARRP   +  AD    +I CD+ D  
Sbjct: 28  VALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSDAE 87

Query: 71  VAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQT 130
                LS +  VTH+F+VTW  +   +S++C + N +ML N L A++P AP+LRHV LQT
Sbjct: 88  DTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCLQT 147

Query: 131 GIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRVAW 190
           G KHY  L       G R +  + E+ PR     NFYY  ED+L E +       + V W
Sbjct: 148 GTKHY--LGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK----KIETVTW 207

Query: 191 SVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDARLVA 250
           S+ RP +I G S  +  N +G+LCVY AICK    P +F G+   W E  +  SDA L+A
Sbjct: 208 SIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAW-EGFMTASDADLIA 267

Query: 251 EQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEMFSPEFNYTAA 310
           EQ +WAA   ++   + EAFN  N   F+WK +W  +AE+ G+     E           
Sbjct: 268 EQQIWAA---VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE-GKNLGLVEM 327

Query: 311 MGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFTLKYKML 370
           M  K  VW E+V+E  L   ++EE+  W   D +  + G M+ S +KS  +GF       
Sbjct: 328 MKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEG-MIDSMNKSKEYGFLGFRNSN 387

Query: 371 DSIFYWIDLMRRDNLIP 385
           +S   WID  +   ++P
Sbjct: 388 NSFISWIDKYKAFKIVP 388

BLAST of Sed0016701 vs. ExPASy Swiss-Prot
Match: A0A221J5X1 ((S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.2e-57
Identity = 140/381 (36.75%), Postives = 202/381 (53.02%), Query Frame = 0

Query: 11  VAVIFGVTGMVGKELAKTL-LGSTAG--WKVYGVARRPDNVSPIADPNFHFIACDLLDRH 70
           VA+I GVTG+VG  LA+ L L  T G  WKVYGVARRP   S   D   ++I+CD+ +  
Sbjct: 29  VALIVGVTGLVGNSLAEMLPLSDTPGGPWKVYGVARRP-RPSWNEDHPINYISCDVSNTA 88

Query: 71  VAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQT 130
                L  +  VTH+F+ TW +  R   ++  + N  ML N LD ++P  P+L+H+ LQT
Sbjct: 89  EVEAKLPPLSDVTHIFYATWTS--RSSEEENCEANGKMLKNVLDTMIPNCPNLKHICLQT 148

Query: 131 GIKHYV-SLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRVA 190
           G  HYV S+    + G    +    E+ PR N+ +NFYY  ED+L E +    G    + 
Sbjct: 149 GRFHYVASVVDWKINGS--HDTPLTEDLPRLNT-NNFYYTQEDILLEEVKRKEG----LT 208

Query: 191 WSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDARLV 250
           WSV RPG I G S  +  N +G+LCVY AICK+      F G    W     D +DA L+
Sbjct: 209 WSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKQEGAVLRFPGCKGAWDGYS-DCADADLI 268

Query: 251 AEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEM-FSPEFNYT 310
           AE ++WAA   ++   + ++FN  NG  F+WK +W  +AE+ GV     E     +    
Sbjct: 269 AEHYIWAA---LDPHAKNQSFNVSNGDVFKWKHLWKVLAEQFGVECGGYEYEEGQQVRLQ 328

Query: 311 AAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFTLKYK 370
             M DKG VW +IV E GL  T++E++  W   D++     ++  S +KS   GF     
Sbjct: 329 DVMKDKGPVWDKIVRENGLSNTKLEDVGKWWFSDTILWNECRL-DSMNKSKEHGFLGFRN 388

Query: 371 MLDSIFYWIDLMRRDNLIPSS 387
             +   YWI  ++   ++PSS
Sbjct: 389 SKNCFLYWIHKLKAYKIVPSS 394

BLAST of Sed0016701 vs. ExPASy Swiss-Prot
Match: A0A1C9CX56 ((S)-8-oxocitronellyl enol synthase CYC1 OS=Camptotheca acuminata OX=16922 GN=CYC1 PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.0e-57
Identity = 144/383 (37.60%), Postives = 199/383 (51.96%), Query Frame = 0

Query: 11  VAVIFGVTGMVGKELAKTL-LGSTAG--WKVYGVARRPDNVSPIADPNFHFIACDLLDRH 70
           V +I GVTG+VG  LA+ L L  T G  WKVYGVARRP   +   D    +I CD+    
Sbjct: 27  VGLIIGVTGIVGNSLAEILPLPDTPGGPWKVYGVARRP-RPAWNTDHPIEYIQCDVASTE 86

Query: 71  VAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQT 130
                LS + ++TH+F+V W       S+DC + N  M  N L AV+P AP+LRH+ +QT
Sbjct: 87  DTLAKLSPLTNITHIFYVAWTG-----SEDC-EVNETMFHNVLKAVIPNAPNLRHICIQT 146

Query: 131 GIKHYVSLTSAAVTGGG--RGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRV 190
           GIKHY   T      GG    +  + E+ PR N+  NFY+ LED+L E  +   G    +
Sbjct: 147 GIKHY---TGPLDPDGGIQPHDSPFTEDLPRLNA-PNFYHNLEDILIEEAAKKKG----L 206

Query: 191 AWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGG----TAACWSEVCIDGS 250
            WSV RP L+ G S  +  N +G+L VY  ICK   KP ++ G    +   W+ V  D +
Sbjct: 207 TWSVHRPALVFGFSPYSMMNIIGALSVYAVICKHENKPLIYHGAPDNSRDSWN-VYADAA 266

Query: 251 DARLVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEMFSPE 310
           DA L+AE  +WAA   ++ + + E FN  NG  F+WK++W  +AE+  V     E     
Sbjct: 267 DADLIAEHQIWAA---VDSNAKNEVFNCSNGDVFKWKQMWKILAEQFEVELVGYEDGQKR 326

Query: 311 FNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFT 370
            +    M  KG VW EIV +  L PT++E++A W   D +      +V   +KS   GF 
Sbjct: 327 MSLQERMKGKGPVWDEIVRKHKLLPTKLEDVAQWWFAD-IVSQSQNLVNGVNKSKENGFL 386

Query: 371 LKYKMLDSIFYWIDLMRRDNLIP 385
                  S  YWI  MR   +IP
Sbjct: 387 GFRDSKKSFVYWIKKMRAAKIIP 389

BLAST of Sed0016701 vs. ExPASy Swiss-Prot
Match: Q6PQJ9 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 3.4e-57
Identity = 144/377 (38.20%), Postives = 196/377 (51.99%), Query Frame = 0

Query: 11  VAVIFGVTGMVGKELAKTL-LGSTAG--WKVYGVARRPDNVSPIADPNFHFIACDLLDRH 70
           VA+I GVTG++G  LA+ L L  T G  WKVYGVARR        +P  +++ CD+ D  
Sbjct: 28  VALIVGVTGIIGNSLAEILPLADTPGGPWKVYGVARRTRPAWHEDNP-INYVQCDISDPD 87

Query: 71  VAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQT 130
            +   LS +  VTH+F+VTWA   R   Q+  + N  M  N LDAV+P  P+L+H+SLQT
Sbjct: 88  DSQAKLSPLTDVTHVFYVTWAN--RSTEQENCEANSKMFRNVLDAVIPNCPNLKHISLQT 147

Query: 131 GIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRVAW 190
           G KHY+    +        +  Y E+ PR     NFYY LED++ E +    G    + W
Sbjct: 148 GRKHYMGPFESYGKIESH-DPPYTEDLPRLKY-MNFYYDLEDIMLEEVEKKEG----LTW 207

Query: 191 SVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDARLVA 250
           SV RPG I G S  +  N +G+LCVY AICK   K   F G  A W     D SDA L+A
Sbjct: 208 SVHRPGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLRFTGCKAAWDGYS-DCSDADLIA 267

Query: 251 EQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEMFSPEFNYTAA 310
           E H+WAA   ++   + EAFN  NG  F+WK  W  +AE+ GV     E    +      
Sbjct: 268 EHHIWAA---VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVGCGEYEE-GVDLKLQDL 327

Query: 311 MGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFTLKYKML 370
           M  K  VW EIV E GL PT+++++  W   D +       + S +KS   GF       
Sbjct: 328 MKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDVILG-NECFLDSMNKSKEHGFLGFRNSK 387

Query: 371 DSIFYWIDLMRRDNLIP 385
           ++   WID  +   ++P
Sbjct: 388 NAFISWIDKAKAYKIVP 389

BLAST of Sed0016701 vs. ExPASy TrEMBL
Match: A0A6J1K9J8 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492905 PE=3 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 2.1e-182
Identity = 319/395 (80.76%), Postives = 344/395 (87.09%), Query Frame = 0

Query: 1   MAAETINPK------LVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
           MAAETIN K      LVAVIFGVTG+VGKELAKTLL ST GWKVYGVARRPDNVSPI D 
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKTLL-STGGWKVYGVARRPDNVSPITDT 60

Query: 61  NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
           NF FI+CDLLDR  AH+NLS +RHVTHLFWVTW AQLRFD+ DC+DQNR MLAN LD +L
Sbjct: 61  NFQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWTAQLRFDTSDCSDQNRTMLANVLDVIL 120

Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEV-VYCEESPRANSGSNFYYALEDLLRE 180
           P AP LRHVSLQTGIKHYVSLT AAV GGG G + VYCE+SPRA SG+NFYYALEDLLRE
Sbjct: 121 PAAPALRHVSLQTGIKHYVSLTGAAVDGGGGGGMAVYCEDSPRAESGNNFYYALEDLLRE 180

Query: 181 RLSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACW 240
           RLS   GGG+RVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGG+AACW
Sbjct: 181 RLS---GGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACW 240

Query: 241 SEVCIDGSDARLVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAA 300
              CI+GSDARLVAEQHVWAAT +IN + +GEAFN CNGWSF WKEIWGAVAEK G+AAA
Sbjct: 241 EGFCINGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGLAAA 300

Query: 301 AAE----MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMV 360
           AAE    MFSPEF+YTAAM +KG+VWAEIVEEEGL PTEMEELANWG +DSLFRLPGKMV
Sbjct: 301 AAEDEKGMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMV 360

Query: 361 ASRHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIP 385
           ASR+KSDR GF++KYKMLDSI YWID+MR D LIP
Sbjct: 361 ASRNKSDRLGFSVKYKMLDSILYWIDVMRYDTLIP 391

BLAST of Sed0016701 vs. ExPASy TrEMBL
Match: A0A6J1GA93 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111452342 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 5.1e-181
Identity = 315/393 (80.15%), Postives = 340/393 (86.51%), Query Frame = 0

Query: 1   MAAETINPK------LVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
           MAAETIN K      LVAVIFGVTG+VGKELAK LL ST GWKVYGVARRPDNVSPI D 
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALL-STGGWKVYGVARRPDNVSPITDT 60

Query: 61  NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
           NF FI+CDLLDR  AH+NLS +RHVTH+FWVTWAAQLRFD+ DC+DQNR MLAN LD +L
Sbjct: 61  NFQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVIL 120

Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRER 180
           P AP LRHVSLQTGIKHYVSLT AAV GGG    VYCE+SPRA SG+NFYYALEDLLRER
Sbjct: 121 PAAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRER 180

Query: 181 LSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWS 240
           LS   GGG+RVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGG+AACW 
Sbjct: 181 LS---GGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWE 240

Query: 241 EVCIDGSDARLVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAA 300
             CI+GSDARLVAEQHVWAAT +IN + +GEAFN CNGWSF WKEIWGAVAEK GVAAAA
Sbjct: 241 GFCINGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAA 300

Query: 301 AE---MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVAS 360
            +   MFSPEF+YTAAM +KG +WAEIVEEEGL PTEMEELANWG +DSLFRLPGKMVAS
Sbjct: 301 EDEEGMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVAS 360

Query: 361 RHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIP 385
           R+KSDR GF++KYKMLDSI YWID+MR D LIP
Sbjct: 361 RNKSDRLGFSVKYKMLDSILYWIDVMRYDKLIP 389

BLAST of Sed0016701 vs. ExPASy TrEMBL
Match: A0A0A0LVI2 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 PE=3 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 4.1e-175
Identity = 311/400 (77.75%), Postives = 336/400 (84.00%), Query Frame = 0

Query: 1   MAAETINP------KLVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
           MA E IN       +LVAVIFGVTG+VGK+LAKTLL STAGWKVYGVARRPDNVSPI+ P
Sbjct: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLL-STAGWKVYGVARRPDNVSPISHP 60

Query: 61  NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
            FHFI+CDLLDR    QNLS +RHVTHLFW+TWAAQLR DS DC+DQNRAMLANALDA+L
Sbjct: 61  KFHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAIL 120

Query: 121 PTAPDLRHVSLQTGIKHYVSLT--SAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLR 180
           P+AP LRHVSLQTGIKHY SLT  +A   GGG GEVVYCE+SPRA SG+NFYY LEDLLR
Sbjct: 121 PSAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLR 180

Query: 181 ERLSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAAC 240
           ERLS    G + VAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLK PFVFGGTAAC
Sbjct: 181 ERLS---CGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAAC 240

Query: 241 WSEVCIDGSDARLVAEQHVWAATK--QINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGV 300
           W EV IDGSDARL AEQH+W ATK  +IN + +GEAFN CNGWSFRWKEIWGAVAEKLGV
Sbjct: 241 WEEVYIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGV 300

Query: 301 AAAAAE-----MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLP 360
           A   AE     MFS EF+YTAAMGDKG+VWAEIVEEEGL  TEMEELANWG +D+LFRLP
Sbjct: 301 ATTGAEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLP 360

Query: 361 GKMVASRHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIPS 386
            KMVASR KSDR GF +KYKMLDS+ YWID+MR D LIPS
Sbjct: 361 EKMVASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLIPS 396

BLAST of Sed0016701 vs. ExPASy TrEMBL
Match: A0A6J1HM54 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111464842 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 8.6e-173
Identity = 304/383 (79.37%), Postives = 328/383 (85.64%), Query Frame = 0

Query: 7   NPKLVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADPNFHFIACDLLDR 66
           N +LVAVIFGVTG+VGKELAK LL STAGWKVYGVARRPDN+SPI+DPNFHFI+CDLLDR
Sbjct: 13  NGELVAVIFGVTGLVGKELAKMLL-STAGWKVYGVARRPDNISPISDPNFHFISCDLLDR 72

Query: 67  HVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQ 126
             AH NLS + HVTH+FW+TWAAQLRFDSQDC+DQN+AMLANALDA+LP+AP LRHVSLQ
Sbjct: 73  RSAHLNLSPLGHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILPSAPALRHVSLQ 132

Query: 127 TGIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRVA 186
           TG+KHY SLTS+  TG G GEVVY E SPRA SG+NFYY LEDLLRERLS   G  + VA
Sbjct: 133 TGVKHYASLTSS--TGSGGGEVVYSENSPRAESGNNFYYVLEDLLRERLS---GRRRVVA 192

Query: 187 WSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDARLV 246
           WS+LRPGLILGCSNRTFFNFMGSLCVYGAICKKLK PFVFGGTAACW EV ID SDARL 
Sbjct: 193 WSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDSSDARLT 252

Query: 247 AEQHVWAATK--QINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAE--MFSPEF 306
           AEQHVWAATK  +IN   +GEAFN CNGWSF WKEIWGAVAEKLGV   A +  MFSPEF
Sbjct: 253 AEQHVWAATKAVEINALTDGEAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDERMFSPEF 312

Query: 307 NYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFTL 366
            YTAAM +KG VWAEIVEEEGL  TEM ELANWG +DSLFRLP KMVASR KSDR GFT+
Sbjct: 313 RYTAAMAEKGTVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTV 372

Query: 367 KYKMLDSIFYWIDLMRRDNLIPS 386
           KYKMLDSI YWID+MR D LIPS
Sbjct: 373 KYKMLDSILYWIDVMRHDKLIPS 389

BLAST of Sed0016701 vs. ExPASy TrEMBL
Match: A0A5D3BGL7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001240 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.5e-172
Identity = 307/397 (77.33%), Postives = 332/397 (83.63%), Query Frame = 0

Query: 1   MAAETINP------KLVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
           MAA+ IN       +LVAVIFGVTG+VGK+LAKTLL STAGWKVYGVARRPDNVSPI+ P
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLL-STAGWKVYGVARRPDNVSPISHP 60

Query: 61  NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
            FHFI+CDLLDR    QNLS +RHVTHLFW+TWAAQLR DS  C+DQNRAMLANALDA+L
Sbjct: 61  KFHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAIL 120

Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRER 180
           P+AP LRHVSLQTG+KHY SLT  A   GGR EVVYCE+SPRA SG+NFYY LEDLLRER
Sbjct: 121 PSAPALRHVSLQTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRER 180

Query: 181 LSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWS 240
           LS    G + VAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLK PFVFGGTAACW 
Sbjct: 181 LS---CGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWE 240

Query: 241 EVCIDGSDARLVAEQHVWAATK--QINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAA 300
           EV IDGSDARL AEQH+W ATK  +IN + +GEAFN CNGWSFRWKEIWG VAEKLGVA 
Sbjct: 241 EVYIDGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVAT 300

Query: 301 AAAE----MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKM 360
            A E    MFS EF+YTAAMGDKG+VWAEIVEEEGL  TEMEELANW  +D+LFRLP KM
Sbjct: 301 TAEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKM 360

Query: 361 VASRHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIPS 386
           VASR KSDRFGF +KYKMLDSI YWID+MR D LIPS
Sbjct: 361 VASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 393

BLAST of Sed0016701 vs. TAIR 10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 395.2 bits (1014), Expect = 6.0e-110
Identity = 200/376 (53.19%), Postives = 254/376 (67.55%), Query Frame = 0

Query: 11  VAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADPNFHFIACDLLDRHVAH 70
           VA+IFGVTG+VG+E+ KTLL S  GW++YGVAR P+  S      ++FI+CDLL+     
Sbjct: 18  VALIFGVTGLVGREIVKTLLMSKPGWRIYGVARNPEINS--MTKMYNFISCDLLNASETK 77

Query: 71  QNLSQIRH-VTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQTGI 130
           Q LS ++  V+H+FWVTW+ +   D+ +C  QN+ ML NALDA+LP A  L+H SLQTG+
Sbjct: 78  QRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSLQTGM 137

Query: 131 KHYVSLTSAAVT-GGGRGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRVAWS 190
           KHYVSL    +  G G     Y EE PR +SG NFYY LEDLL+E+++        V WS
Sbjct: 138 KHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKITRSS-----VVWS 197

Query: 191 VLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDARLVAE 250
           V RPGL++G S+RT +NFMGSLCVYGA+CK L  PFVFGGT  CW E  IDGSD+ LVAE
Sbjct: 198 VQRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLVAE 257

Query: 251 QHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEMFSPEFNYTAAM 310
           QH++AAT       +GEAFNA NG  F WKEIW  + +KLGV      MF   F +   M
Sbjct: 258 QHIFAATSG-KVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQVNETTMFDEGFWFGREM 317

Query: 311 GDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFTLKYKMLD 370
            ++  VW EIV +E L  TE+E+LANW  +D+LFR P K++  R K DRFGF  KY+ LD
Sbjct: 318 VERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYRTLD 377

Query: 371 SIFYWIDLMRRDNLIP 385
           S+ YWID+MR + LIP
Sbjct: 378 SVLYWIDVMRDEKLIP 385

BLAST of Sed0016701 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 234.2 bits (596), Expect = 1.8e-61
Identity = 146/377 (38.73%), Postives = 202/377 (53.58%), Query Frame = 0

Query: 11  VAVIFGVTGMVGKELAKTL-LGSTAG--WKVYGVARRPDNVSPIADPNFHFIACDLLDRH 70
           VA+I GVTG+VG  LA+ L L  T G  WKVYGVARRP   +  AD    +I CD+ D  
Sbjct: 28  VALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSDAE 87

Query: 71  VAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQT 130
                LS +  VTH+F+VTW  +   +S++C + N +ML N L A++P AP+LRHV LQT
Sbjct: 88  DTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCLQT 147

Query: 131 GIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRVAW 190
           G KHY  L       G R +  + E+ PR     NFYY  ED+L E +       + V W
Sbjct: 148 GTKHY--LGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK----KIETVTW 207

Query: 191 SVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDARLVA 250
           S+ RP +I G S  +  N +G+LCVY AICK    P +F G+   W E  +  SDA L+A
Sbjct: 208 SIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAW-EGFMTASDADLIA 267

Query: 251 EQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEMFSPEFNYTAA 310
           EQ +WAA   ++   + EAFN  N   F+WK +W  +AE+ G+     E           
Sbjct: 268 EQQIWAA---VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE-GKNLGLVEM 327

Query: 311 MGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFTLKYKML 370
           M  K  VW E+V+E  L   ++EE+  W   D +  + G M+ S +KS  +GF       
Sbjct: 328 MKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEG-MIDSMNKSKEYGFLGFRNSN 387

Query: 371 DSIFYWIDLMRRDNLIP 385
           +S   WID  +   ++P
Sbjct: 388 NSFISWIDKYKAFKIVP 388

BLAST of Sed0016701 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 234.2 bits (596), Expect = 1.8e-61
Identity = 146/377 (38.73%), Postives = 202/377 (53.58%), Query Frame = 0

Query: 11  VAVIFGVTGMVGKELAKTL-LGSTAG--WKVYGVARRPDNVSPIADPNFHFIACDLLDRH 70
           VA+I GVTG+VG  LA+ L L  T G  WKVYGVARRP   +  AD    +I CD+ D  
Sbjct: 27  VALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSDAE 86

Query: 71  VAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQT 130
                LS +  VTH+F+VTW  +   +S++C + N +ML N L A++P AP+LRHV LQT
Sbjct: 87  DTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCLQT 146

Query: 131 GIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRVAW 190
           G KHY  L       G R +  + E+ PR     NFYY  ED+L E +       + V W
Sbjct: 147 GTKHY--LGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK----KIETVTW 206

Query: 191 SVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDARLVA 250
           S+ RP +I G S  +  N +G+LCVY AICK    P +F G+   W E  +  SDA L+A
Sbjct: 207 SIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAW-EGFMTASDADLIA 266

Query: 251 EQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEMFSPEFNYTAA 310
           EQ +WAA   ++   + EAFN  N   F+WK +W  +AE+ G+     E           
Sbjct: 267 EQQIWAA---VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE-GKNLGLVEM 326

Query: 311 MGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFTLKYKML 370
           M  K  VW E+V+E  L   ++EE+  W   D +  + G M+ S +KS  +GF       
Sbjct: 327 MKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEG-MIDSMNKSKEYGFLGFRNSN 386

Query: 371 DSIFYWIDLMRRDNLIP 385
           +S   WID  +   ++P
Sbjct: 387 NSFISWIDKYKAFKIVP 387

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022998181.14.2e-18280.763-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima][more]
XP_022948773.11.0e-18080.153-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata][more]
KAG6607304.14.0e-18080.15(S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. ... [more]
KAG7036983.16.8e-18080.153-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp... [more]
XP_038895390.12.6e-17980.10(S)-8-oxocitronellyl enol synthase CYC2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A1C9CX666.4e-6440.42(S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC... [more]
Q9STX22.5e-6038.733-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP... [more]
A0A221J5X11.2e-5736.75(S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 ... [more]
A0A1C9CX562.0e-5737.60(S)-8-oxocitronellyl enol synthase CYC1 OS=Camptotheca acuminata OX=16922 GN=CYC... [more]
Q6PQJ93.4e-5738.203-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1K9J82.1e-18280.763-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GA935.1e-18180.153-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A0A0LVI24.1e-17577.75Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 P... [more]
A0A6J1HM548.6e-17379.373-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A5D3BGL72.5e-17277.333-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo var. makuwa... [more]
Match NameE-valueIdentityDescription
AT5G58750.16.0e-11053.19NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G24220.11.8e-6138.73NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G24220.21.8e-6138.73NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 5..384
e-value: 2.8E-97
score: 328.1
NoneNo IPR availablePANTHERPTHR32487:SF123-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE ISOFORM X1coord: 7..384
NoneNo IPR availablePANTHERPTHR324873-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASEcoord: 7..384
NoneNo IPR availableCDDcd089485beta-POR_like_SDR_acoord: 11..327
e-value: 1.05138E-97
score: 291.457
IPR001509NAD-dependent epimerase/dehydratasePFAMPF01370Epimerasecoord: 13..156
e-value: 2.4E-8
score: 33.8
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 11..293

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0016701.1Sed0016701.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity