Homology
BLAST of Sed0016701 vs. NCBI nr
Match:
XP_022998181.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima])
HSP 1 Score: 648.3 bits (1671), Expect = 4.2e-182
Identity = 319/395 (80.76%), Postives = 344/395 (87.09%), Query Frame = 0
Query: 1 MAAETINPK------LVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
MAAETIN K LVAVIFGVTG+VGKELAKTLL ST GWKVYGVARRPDNVSPI D
Sbjct: 1 MAAETINAKLDGNGELVAVIFGVTGLVGKELAKTLL-STGGWKVYGVARRPDNVSPITDT 60
Query: 61 NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
NF FI+CDLLDR AH+NLS +RHVTHLFWVTW AQLRFD+ DC+DQNR MLAN LD +L
Sbjct: 61 NFQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWTAQLRFDTSDCSDQNRTMLANVLDVIL 120
Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEV-VYCEESPRANSGSNFYYALEDLLRE 180
P AP LRHVSLQTGIKHYVSLT AAV GGG G + VYCE+SPRA SG+NFYYALEDLLRE
Sbjct: 121 PAAPALRHVSLQTGIKHYVSLTGAAVDGGGGGGMAVYCEDSPRAESGNNFYYALEDLLRE 180
Query: 181 RLSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACW 240
RLS GGG+RVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGG+AACW
Sbjct: 181 RLS---GGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACW 240
Query: 241 SEVCIDGSDARLVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAA 300
CI+GSDARLVAEQHVWAAT +IN + +GEAFN CNGWSF WKEIWGAVAEK G+AAA
Sbjct: 241 EGFCINGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGLAAA 300
Query: 301 AAE----MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMV 360
AAE MFSPEF+YTAAM +KG+VWAEIVEEEGL PTEMEELANWG +DSLFRLPGKMV
Sbjct: 301 AAEDEKGMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMV 360
Query: 361 ASRHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIP 385
ASR+KSDR GF++KYKMLDSI YWID+MR D LIP
Sbjct: 361 ASRNKSDRLGFSVKYKMLDSILYWIDVMRYDTLIP 391
BLAST of Sed0016701 vs. NCBI nr
Match:
XP_022948773.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata])
HSP 1 Score: 643.7 bits (1659), Expect = 1.0e-180
Identity = 315/393 (80.15%), Postives = 340/393 (86.51%), Query Frame = 0
Query: 1 MAAETINPK------LVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
MAAETIN K LVAVIFGVTG+VGKELAK LL ST GWKVYGVARRPDNVSPI D
Sbjct: 1 MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALL-STGGWKVYGVARRPDNVSPITDT 60
Query: 61 NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
NF FI+CDLLDR AH+NLS +RHVTH+FWVTWAAQLRFD+ DC+DQNR MLAN LD +L
Sbjct: 61 NFQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVIL 120
Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRER 180
P AP LRHVSLQTGIKHYVSLT AAV GGG VYCE+SPRA SG+NFYYALEDLLRER
Sbjct: 121 PAAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRER 180
Query: 181 LSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWS 240
LS GGG+RVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGG+AACW
Sbjct: 181 LS---GGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWE 240
Query: 241 EVCIDGSDARLVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAA 300
CI+GSDARLVAEQHVWAAT +IN + +GEAFN CNGWSF WKEIWGAVAEK GVAAAA
Sbjct: 241 GFCINGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAA 300
Query: 301 AE---MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVAS 360
+ MFSPEF+YTAAM +KG +WAEIVEEEGL PTEMEELANWG +DSLFRLPGKMVAS
Sbjct: 301 EDEEGMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVAS 360
Query: 361 RHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIP 385
R+KSDR GF++KYKMLDSI YWID+MR D LIP
Sbjct: 361 RNKSDRLGFSVKYKMLDSILYWIDVMRYDKLIP 389
BLAST of Sed0016701 vs. NCBI nr
Match:
KAG6607304.1 ((S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 641.7 bits (1654), Expect = 4.0e-180
Identity = 315/393 (80.15%), Postives = 339/393 (86.26%), Query Frame = 0
Query: 1 MAAETINPK------LVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
M AETIN K LVAVIFGVTG+VGKELAK LL ST GWKVYGVARRPDNVSPI D
Sbjct: 1 MTAETINAKLDGNGELVAVIFGVTGLVGKELAKALL-STGGWKVYGVARRPDNVSPITDT 60
Query: 61 NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
NF FI+CDLLDR A++NLS +RHVTHLFWVTWAAQLRFD+ DC+DQNR MLAN LD +L
Sbjct: 61 NFQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVIL 120
Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRER 180
P AP LRHVSLQTGIKHYVSLT AAV GGG VYCE+SPRA SG+NFYYALEDLLRER
Sbjct: 121 PAAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRER 180
Query: 181 LSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWS 240
LS GGG+RVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGG+AACW
Sbjct: 181 LS---GGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWE 240
Query: 241 EVCIDGSDARLVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAA 300
CI+GSDARLVAEQHVWAAT +IN + +GEAFN CNGWSF WKEIWGAVAEK GVAAAA
Sbjct: 241 GFCINGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAA 300
Query: 301 AE---MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVAS 360
+ MFSPEF+YTAAM +KG VWAEIVEEEGL PTEMEELANWG +DSLFRLPGKMVAS
Sbjct: 301 EDEEGMFSPEFHYTAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVAS 360
Query: 361 RHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIP 385
R+KSDR GF++KYKMLDSI YWID+MR D LIP
Sbjct: 361 RNKSDRLGFSVKYKMLDSILYWIDVMRYDKLIP 389
BLAST of Sed0016701 vs. NCBI nr
Match:
KAG7036983.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 641.0 bits (1652), Expect = 6.8e-180
Identity = 315/393 (80.15%), Postives = 339/393 (86.26%), Query Frame = 0
Query: 1 MAAETINPK------LVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
MAAETIN K LVAVIFGVTG+VGKELAK LL ST GWKVYGVARRPDNVSPI D
Sbjct: 1 MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALL-STGGWKVYGVARRPDNVSPITDT 60
Query: 61 NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
NF FI+CDLLDR A++NLS +RHVTHLFWVTWAAQLRFD+ DC+DQNR MLAN LD +L
Sbjct: 61 NFQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVIL 120
Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRER 180
P AP LRHVSLQTGIKHYVSLT AAV GGG VYCE+SPRA SG+NFYYALEDLLRER
Sbjct: 121 PAAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRER 180
Query: 181 LSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWS 240
LS GGG+RVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGG+AACW
Sbjct: 181 LS---GGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWE 240
Query: 241 EVCIDGSDARLVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAA 300
CI+GSDARLVAEQHVWAAT +IN + +GEAFN CNGWSF WKEIWGAVAEK GVAAAA
Sbjct: 241 GFCINGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAA 300
Query: 301 AE---MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVAS 360
+ MFSPEF+Y AAM +KG VWAEIVEEEGL PTEMEELANWG +DSLFRLPGKMVAS
Sbjct: 301 EDEEGMFSPEFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVAS 360
Query: 361 RHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIP 385
R+KSDR GF++KYKMLDSI YWID+MR D LIP
Sbjct: 361 RNKSDRLGFSVKYKMLDSILYWIDVMRYDKLIP 389
BLAST of Sed0016701 vs. NCBI nr
Match:
XP_038895390.1 ((S)-8-oxocitronellyl enol synthase CYC2 [Benincasa hispida])
HSP 1 Score: 639.0 bits (1647), Expect = 2.6e-179
Identity = 318/397 (80.10%), Postives = 341/397 (85.89%), Query Frame = 0
Query: 1 MAAETI------NPKLVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
MAAE I N +LVAVIFGVTG+VGKELAKTLL STAGWKVYGVARRPDN+SPI+ P
Sbjct: 1 MAAEIINTNLVANAELVAVIFGVTGLVGKELAKTLL-STAGWKVYGVARRPDNISPISHP 60
Query: 61 NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
NFHFI+CDLLDR AH NLS +RHVTHLFW+TWAAQLRFDSQ C+DQNRAMLANALDA+L
Sbjct: 61 NFHFISCDLLDRRSAHSNLSPVRHVTHLFWITWAAQLRFDSQGCSDQNRAMLANALDAIL 120
Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRER 180
P+AP LRHVSLQTGIKHY SLT A GG GEV YCE+SPRA SG+NFYY LEDLLRER
Sbjct: 121 PSAPALRHVSLQTGIKHYASLTRLAAGCGGGGEVAYCEDSPRAESGNNFYYVLEDLLRER 180
Query: 181 LSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWS 240
LS GG + V WSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLK PFVFGGTAACW
Sbjct: 181 LS---GGRRVVGWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWE 240
Query: 241 EVCIDGSDARLVAEQHVWAATK--QINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAA 300
EV IDGSDARL AEQHVWAATK +IN + +GEAFN CNGWSFRWKEIWGAVAEKLGVAA
Sbjct: 241 EVYIDGSDARLTAEQHVWAATKGIKINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVAA 300
Query: 301 AAAE----MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKM 360
A E MFS EF+YTAAMGDKG++WAEIVEEEGL PTEMEELANWG +D+LFRLP KM
Sbjct: 301 AEKEDERIMFSAEFHYTAAMGDKGKLWAEIVEEEGLVPTEMEELANWGFLDTLFRLPEKM 360
Query: 361 VASRHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIPS 386
VASR KSDRFGFT+KYKMLDSI YWID+MR D LIPS
Sbjct: 361 VASRAKSDRFGFTVKYKMLDSILYWIDVMRNDKLIPS 393
BLAST of Sed0016701 vs. ExPASy Swiss-Prot
Match:
A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)
HSP 1 Score: 246.1 bits (627), Expect = 6.4e-64
Identity = 154/381 (40.42%), Postives = 208/381 (54.59%), Query Frame = 0
Query: 11 VAVIFGVTGMVGKELAKTL-LGSTAG--WKVYGVARRPDNVSPIADPNFHFIACDLLDRH 70
VA+I GVTG+VG LA+ L + T G WKVYGVARRP + AD +I CD+ D
Sbjct: 28 VALIIGVTGIVGNSLAEILPISDTPGGPWKVYGVARRP-RPAWNADHPVEYIQCDISDAS 87
Query: 71 VAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQT 130
H LS + VTH+FWVTWA R +C + N ML N L+A++P A +L H+ LQT
Sbjct: 88 DTHTKLSPLTDVTHIFWVTWAN--RPTESECCELNGTMLRNVLNALIPKAANLHHICLQT 147
Query: 131 GIKHYVSLTSAAVTGGGR---GEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKR 190
G KHY+ A G+ E + E+ PR N+ NFYY LED+L E G
Sbjct: 148 GHKHYIGPFEAF----GKIKPHEPPFTEDMPRLNA-PNFYYTLEDMLVEASEKKAG---- 207
Query: 191 VAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDAR 250
+ WSV RP +I G S + N +G+LCVY AICK P F GT A W+ + SDA
Sbjct: 208 LNWSVHRPAVIFGFSPFSMMNIIGTLCVYAAICKHENTPLKFPGTKAAWNCYSV-ASDAD 267
Query: 251 LVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEMFSPEFNY 310
L+AE +WAA ++ + EAFN NG F+WK +W +AE+ GV A + +
Sbjct: 268 LIAEHQIWAA---VDPYAKNEAFNCSNGDLFKWKHLWKVLAEQFGVEYAEFDESEKPTSL 327
Query: 311 TAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGK-MVASRHKSDRFGFTLK 370
M DKG VW EIV E GL T++E +A W D + L G+ ++ S +KS G+
Sbjct: 328 VERMKDKGPVWEEIVRENGLHTTKLEGVATWWFADVI--LGGECLLDSMNKSKEHGYLGF 387
Query: 371 YKMLDSIFYWIDLMRRDNLIP 385
+S+ ID M+ ++P
Sbjct: 388 RNTKNSLISVIDKMKAHKIVP 390
BLAST of Sed0016701 vs. ExPASy Swiss-Prot
Match:
Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)
HSP 1 Score: 234.2 bits (596), Expect = 2.5e-60
Identity = 146/377 (38.73%), Postives = 202/377 (53.58%), Query Frame = 0
Query: 11 VAVIFGVTGMVGKELAKTL-LGSTAG--WKVYGVARRPDNVSPIADPNFHFIACDLLDRH 70
VA+I GVTG+VG LA+ L L T G WKVYGVARRP + AD +I CD+ D
Sbjct: 28 VALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSDAE 87
Query: 71 VAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQT 130
LS + VTH+F+VTW + +S++C + N +ML N L A++P AP+LRHV LQT
Sbjct: 88 DTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCLQT 147
Query: 131 GIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRVAW 190
G KHY L G R + + E+ PR NFYY ED+L E + + V W
Sbjct: 148 GTKHY--LGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK----KIETVTW 207
Query: 191 SVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDARLVA 250
S+ RP +I G S + N +G+LCVY AICK P +F G+ W E + SDA L+A
Sbjct: 208 SIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAW-EGFMTASDADLIA 267
Query: 251 EQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEMFSPEFNYTAA 310
EQ +WAA ++ + EAFN N F+WK +W +AE+ G+ E
Sbjct: 268 EQQIWAA---VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE-GKNLGLVEM 327
Query: 311 MGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFTLKYKML 370
M K VW E+V+E L ++EE+ W D + + G M+ S +KS +GF
Sbjct: 328 MKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEG-MIDSMNKSKEYGFLGFRNSN 387
Query: 371 DSIFYWIDLMRRDNLIP 385
+S WID + ++P
Sbjct: 388 NSFISWIDKYKAFKIVP 388
BLAST of Sed0016701 vs. ExPASy Swiss-Prot
Match:
A0A221J5X1 ((S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 SV=1)
HSP 1 Score: 225.3 bits (573), Expect = 1.2e-57
Identity = 140/381 (36.75%), Postives = 202/381 (53.02%), Query Frame = 0
Query: 11 VAVIFGVTGMVGKELAKTL-LGSTAG--WKVYGVARRPDNVSPIADPNFHFIACDLLDRH 70
VA+I GVTG+VG LA+ L L T G WKVYGVARRP S D ++I+CD+ +
Sbjct: 29 VALIVGVTGLVGNSLAEMLPLSDTPGGPWKVYGVARRP-RPSWNEDHPINYISCDVSNTA 88
Query: 71 VAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQT 130
L + VTH+F+ TW + R ++ + N ML N LD ++P P+L+H+ LQT
Sbjct: 89 EVEAKLPPLSDVTHIFYATWTS--RSSEEENCEANGKMLKNVLDTMIPNCPNLKHICLQT 148
Query: 131 GIKHYV-SLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRVA 190
G HYV S+ + G + E+ PR N+ +NFYY ED+L E + G +
Sbjct: 149 GRFHYVASVVDWKINGS--HDTPLTEDLPRLNT-NNFYYTQEDILLEEVKRKEG----LT 208
Query: 191 WSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDARLV 250
WSV RPG I G S + N +G+LCVY AICK+ F G W D +DA L+
Sbjct: 209 WSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKQEGAVLRFPGCKGAWDGYS-DCADADLI 268
Query: 251 AEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEM-FSPEFNYT 310
AE ++WAA ++ + ++FN NG F+WK +W +AE+ GV E +
Sbjct: 269 AEHYIWAA---LDPHAKNQSFNVSNGDVFKWKHLWKVLAEQFGVECGGYEYEEGQQVRLQ 328
Query: 311 AAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFTLKYK 370
M DKG VW +IV E GL T++E++ W D++ ++ S +KS GF
Sbjct: 329 DVMKDKGPVWDKIVRENGLSNTKLEDVGKWWFSDTILWNECRL-DSMNKSKEHGFLGFRN 388
Query: 371 MLDSIFYWIDLMRRDNLIPSS 387
+ YWI ++ ++PSS
Sbjct: 389 SKNCFLYWIHKLKAYKIVPSS 394
BLAST of Sed0016701 vs. ExPASy Swiss-Prot
Match:
A0A1C9CX56 ((S)-8-oxocitronellyl enol synthase CYC1 OS=Camptotheca acuminata OX=16922 GN=CYC1 PE=1 SV=1)
HSP 1 Score: 224.6 bits (571), Expect = 2.0e-57
Identity = 144/383 (37.60%), Postives = 199/383 (51.96%), Query Frame = 0
Query: 11 VAVIFGVTGMVGKELAKTL-LGSTAG--WKVYGVARRPDNVSPIADPNFHFIACDLLDRH 70
V +I GVTG+VG LA+ L L T G WKVYGVARRP + D +I CD+
Sbjct: 27 VGLIIGVTGIVGNSLAEILPLPDTPGGPWKVYGVARRP-RPAWNTDHPIEYIQCDVASTE 86
Query: 71 VAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQT 130
LS + ++TH+F+V W S+DC + N M N L AV+P AP+LRH+ +QT
Sbjct: 87 DTLAKLSPLTNITHIFYVAWTG-----SEDC-EVNETMFHNVLKAVIPNAPNLRHICIQT 146
Query: 131 GIKHYVSLTSAAVTGGG--RGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRV 190
GIKHY T GG + + E+ PR N+ NFY+ LED+L E + G +
Sbjct: 147 GIKHY---TGPLDPDGGIQPHDSPFTEDLPRLNA-PNFYHNLEDILIEEAAKKKG----L 206
Query: 191 AWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGG----TAACWSEVCIDGS 250
WSV RP L+ G S + N +G+L VY ICK KP ++ G + W+ V D +
Sbjct: 207 TWSVHRPALVFGFSPYSMMNIIGALSVYAVICKHENKPLIYHGAPDNSRDSWN-VYADAA 266
Query: 251 DARLVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEMFSPE 310
DA L+AE +WAA ++ + + E FN NG F+WK++W +AE+ V E
Sbjct: 267 DADLIAEHQIWAA---VDSNAKNEVFNCSNGDVFKWKQMWKILAEQFEVELVGYEDGQKR 326
Query: 311 FNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFT 370
+ M KG VW EIV + L PT++E++A W D + +V +KS GF
Sbjct: 327 MSLQERMKGKGPVWDEIVRKHKLLPTKLEDVAQWWFAD-IVSQSQNLVNGVNKSKENGFL 386
Query: 371 LKYKMLDSIFYWIDLMRRDNLIP 385
S YWI MR +IP
Sbjct: 387 GFRDSKKSFVYWIKKMRAAKIIP 389
BLAST of Sed0016701 vs. ExPASy Swiss-Prot
Match:
Q6PQJ9 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)
HSP 1 Score: 223.8 bits (569), Expect = 3.4e-57
Identity = 144/377 (38.20%), Postives = 196/377 (51.99%), Query Frame = 0
Query: 11 VAVIFGVTGMVGKELAKTL-LGSTAG--WKVYGVARRPDNVSPIADPNFHFIACDLLDRH 70
VA+I GVTG++G LA+ L L T G WKVYGVARR +P +++ CD+ D
Sbjct: 28 VALIVGVTGIIGNSLAEILPLADTPGGPWKVYGVARRTRPAWHEDNP-INYVQCDISDPD 87
Query: 71 VAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQT 130
+ LS + VTH+F+VTWA R Q+ + N M N LDAV+P P+L+H+SLQT
Sbjct: 88 DSQAKLSPLTDVTHVFYVTWAN--RSTEQENCEANSKMFRNVLDAVIPNCPNLKHISLQT 147
Query: 131 GIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRVAW 190
G KHY+ + + Y E+ PR NFYY LED++ E + G + W
Sbjct: 148 GRKHYMGPFESYGKIESH-DPPYTEDLPRLKY-MNFYYDLEDIMLEEVEKKEG----LTW 207
Query: 191 SVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDARLVA 250
SV RPG I G S + N +G+LCVY AICK K F G A W D SDA L+A
Sbjct: 208 SVHRPGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLRFTGCKAAWDGYS-DCSDADLIA 267
Query: 251 EQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEMFSPEFNYTAA 310
E H+WAA ++ + EAFN NG F+WK W +AE+ GV E +
Sbjct: 268 EHHIWAA---VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVGCGEYEE-GVDLKLQDL 327
Query: 311 MGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFTLKYKML 370
M K VW EIV E GL PT+++++ W D + + S +KS GF
Sbjct: 328 MKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDVILG-NECFLDSMNKSKEHGFLGFRNSK 387
Query: 371 DSIFYWIDLMRRDNLIP 385
++ WID + ++P
Sbjct: 388 NAFISWIDKAKAYKIVP 389
BLAST of Sed0016701 vs. ExPASy TrEMBL
Match:
A0A6J1K9J8 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492905 PE=3 SV=1)
HSP 1 Score: 648.3 bits (1671), Expect = 2.1e-182
Identity = 319/395 (80.76%), Postives = 344/395 (87.09%), Query Frame = 0
Query: 1 MAAETINPK------LVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
MAAETIN K LVAVIFGVTG+VGKELAKTLL ST GWKVYGVARRPDNVSPI D
Sbjct: 1 MAAETINAKLDGNGELVAVIFGVTGLVGKELAKTLL-STGGWKVYGVARRPDNVSPITDT 60
Query: 61 NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
NF FI+CDLLDR AH+NLS +RHVTHLFWVTW AQLRFD+ DC+DQNR MLAN LD +L
Sbjct: 61 NFQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWTAQLRFDTSDCSDQNRTMLANVLDVIL 120
Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEV-VYCEESPRANSGSNFYYALEDLLRE 180
P AP LRHVSLQTGIKHYVSLT AAV GGG G + VYCE+SPRA SG+NFYYALEDLLRE
Sbjct: 121 PAAPALRHVSLQTGIKHYVSLTGAAVDGGGGGGMAVYCEDSPRAESGNNFYYALEDLLRE 180
Query: 181 RLSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACW 240
RLS GGG+RVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGG+AACW
Sbjct: 181 RLS---GGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACW 240
Query: 241 SEVCIDGSDARLVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAA 300
CI+GSDARLVAEQHVWAAT +IN + +GEAFN CNGWSF WKEIWGAVAEK G+AAA
Sbjct: 241 EGFCINGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGLAAA 300
Query: 301 AAE----MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMV 360
AAE MFSPEF+YTAAM +KG+VWAEIVEEEGL PTEMEELANWG +DSLFRLPGKMV
Sbjct: 301 AAEDEKGMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMV 360
Query: 361 ASRHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIP 385
ASR+KSDR GF++KYKMLDSI YWID+MR D LIP
Sbjct: 361 ASRNKSDRLGFSVKYKMLDSILYWIDVMRYDTLIP 391
BLAST of Sed0016701 vs. ExPASy TrEMBL
Match:
A0A6J1GA93 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111452342 PE=3 SV=1)
HSP 1 Score: 643.7 bits (1659), Expect = 5.1e-181
Identity = 315/393 (80.15%), Postives = 340/393 (86.51%), Query Frame = 0
Query: 1 MAAETINPK------LVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
MAAETIN K LVAVIFGVTG+VGKELAK LL ST GWKVYGVARRPDNVSPI D
Sbjct: 1 MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALL-STGGWKVYGVARRPDNVSPITDT 60
Query: 61 NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
NF FI+CDLLDR AH+NLS +RHVTH+FWVTWAAQLRFD+ DC+DQNR MLAN LD +L
Sbjct: 61 NFQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVIL 120
Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRER 180
P AP LRHVSLQTGIKHYVSLT AAV GGG VYCE+SPRA SG+NFYYALEDLLRER
Sbjct: 121 PAAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRER 180
Query: 181 LSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWS 240
LS GGG+RVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGG+AACW
Sbjct: 181 LS---GGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWE 240
Query: 241 EVCIDGSDARLVAEQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAA 300
CI+GSDARLVAEQHVWAAT +IN + +GEAFN CNGWSF WKEIWGAVAEK GVAAAA
Sbjct: 241 GFCINGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAA 300
Query: 301 AE---MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVAS 360
+ MFSPEF+YTAAM +KG +WAEIVEEEGL PTEMEELANWG +DSLFRLPGKMVAS
Sbjct: 301 EDEEGMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVAS 360
Query: 361 RHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIP 385
R+KSDR GF++KYKMLDSI YWID+MR D LIP
Sbjct: 361 RNKSDRLGFSVKYKMLDSILYWIDVMRYDKLIP 389
BLAST of Sed0016701 vs. ExPASy TrEMBL
Match:
A0A0A0LVI2 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 PE=3 SV=1)
HSP 1 Score: 624.0 bits (1608), Expect = 4.1e-175
Identity = 311/400 (77.75%), Postives = 336/400 (84.00%), Query Frame = 0
Query: 1 MAAETINP------KLVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
MA E IN +LVAVIFGVTG+VGK+LAKTLL STAGWKVYGVARRPDNVSPI+ P
Sbjct: 1 MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLL-STAGWKVYGVARRPDNVSPISHP 60
Query: 61 NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
FHFI+CDLLDR QNLS +RHVTHLFW+TWAAQLR DS DC+DQNRAMLANALDA+L
Sbjct: 61 KFHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAIL 120
Query: 121 PTAPDLRHVSLQTGIKHYVSLT--SAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLR 180
P+AP LRHVSLQTGIKHY SLT +A GGG GEVVYCE+SPRA SG+NFYY LEDLLR
Sbjct: 121 PSAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLR 180
Query: 181 ERLSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAAC 240
ERLS G + VAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLK PFVFGGTAAC
Sbjct: 181 ERLS---CGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAAC 240
Query: 241 WSEVCIDGSDARLVAEQHVWAATK--QINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGV 300
W EV IDGSDARL AEQH+W ATK +IN + +GEAFN CNGWSFRWKEIWGAVAEKLGV
Sbjct: 241 WEEVYIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGV 300
Query: 301 AAAAAE-----MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLP 360
A AE MFS EF+YTAAMGDKG+VWAEIVEEEGL TEMEELANWG +D+LFRLP
Sbjct: 301 ATTGAEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLP 360
Query: 361 GKMVASRHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIPS 386
KMVASR KSDR GF +KYKMLDS+ YWID+MR D LIPS
Sbjct: 361 EKMVASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLIPS 396
BLAST of Sed0016701 vs. ExPASy TrEMBL
Match:
A0A6J1HM54 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111464842 PE=3 SV=1)
HSP 1 Score: 616.3 bits (1588), Expect = 8.6e-173
Identity = 304/383 (79.37%), Postives = 328/383 (85.64%), Query Frame = 0
Query: 7 NPKLVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADPNFHFIACDLLDR 66
N +LVAVIFGVTG+VGKELAK LL STAGWKVYGVARRPDN+SPI+DPNFHFI+CDLLDR
Sbjct: 13 NGELVAVIFGVTGLVGKELAKMLL-STAGWKVYGVARRPDNISPISDPNFHFISCDLLDR 72
Query: 67 HVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQ 126
AH NLS + HVTH+FW+TWAAQLRFDSQDC+DQN+AMLANALDA+LP+AP LRHVSLQ
Sbjct: 73 RSAHLNLSPLGHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILPSAPALRHVSLQ 132
Query: 127 TGIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRVA 186
TG+KHY SLTS+ TG G GEVVY E SPRA SG+NFYY LEDLLRERLS G + VA
Sbjct: 133 TGVKHYASLTSS--TGSGGGEVVYSENSPRAESGNNFYYVLEDLLRERLS---GRRRVVA 192
Query: 187 WSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDARLV 246
WS+LRPGLILGCSNRTFFNFMGSLCVYGAICKKLK PFVFGGTAACW EV ID SDARL
Sbjct: 193 WSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDSSDARLT 252
Query: 247 AEQHVWAATK--QINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAE--MFSPEF 306
AEQHVWAATK +IN +GEAFN CNGWSF WKEIWGAVAEKLGV A + MFSPEF
Sbjct: 253 AEQHVWAATKAVEINALTDGEAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDERMFSPEF 312
Query: 307 NYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFTL 366
YTAAM +KG VWAEIVEEEGL TEM ELANWG +DSLFRLP KMVASR KSDR GFT+
Sbjct: 313 RYTAAMAEKGTVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTV 372
Query: 367 KYKMLDSIFYWIDLMRRDNLIPS 386
KYKMLDSI YWID+MR D LIPS
Sbjct: 373 KYKMLDSILYWIDVMRHDKLIPS 389
BLAST of Sed0016701 vs. ExPASy TrEMBL
Match:
A0A5D3BGL7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001240 PE=3 SV=1)
HSP 1 Score: 614.8 bits (1584), Expect = 2.5e-172
Identity = 307/397 (77.33%), Postives = 332/397 (83.63%), Query Frame = 0
Query: 1 MAAETINP------KLVAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADP 60
MAA+ IN +LVAVIFGVTG+VGK+LAKTLL STAGWKVYGVARRPDNVSPI+ P
Sbjct: 1 MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLL-STAGWKVYGVARRPDNVSPISHP 60
Query: 61 NFHFIACDLLDRHVAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVL 120
FHFI+CDLLDR QNLS +RHVTHLFW+TWAAQLR DS C+DQNRAMLANALDA+L
Sbjct: 61 KFHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAIL 120
Query: 121 PTAPDLRHVSLQTGIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRER 180
P+AP LRHVSLQTG+KHY SLT A GGR EVVYCE+SPRA SG+NFYY LEDLLRER
Sbjct: 121 PSAPALRHVSLQTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRER 180
Query: 181 LSGGGGGGKRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWS 240
LS G + VAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLK PFVFGGTAACW
Sbjct: 181 LS---CGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWE 240
Query: 241 EVCIDGSDARLVAEQHVWAATK--QINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAA 300
EV IDGSDARL AEQH+W ATK +IN + +GEAFN CNGWSFRWKEIWG VAEKLGVA
Sbjct: 241 EVYIDGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVAT 300
Query: 301 AAAE----MFSPEFNYTAAMGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKM 360
A E MFS EF+YTAAMGDKG+VWAEIVEEEGL TEMEELANW +D+LFRLP KM
Sbjct: 301 TAEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKM 360
Query: 361 VASRHKSDRFGFTLKYKMLDSIFYWIDLMRRDNLIPS 386
VASR KSDRFGF +KYKMLDSI YWID+MR D LIPS
Sbjct: 361 VASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 393
BLAST of Sed0016701 vs. TAIR 10
Match:
AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )
HSP 1 Score: 395.2 bits (1014), Expect = 6.0e-110
Identity = 200/376 (53.19%), Postives = 254/376 (67.55%), Query Frame = 0
Query: 11 VAVIFGVTGMVGKELAKTLLGSTAGWKVYGVARRPDNVSPIADPNFHFIACDLLDRHVAH 70
VA+IFGVTG+VG+E+ KTLL S GW++YGVAR P+ S ++FI+CDLL+
Sbjct: 18 VALIFGVTGLVGREIVKTLLMSKPGWRIYGVARNPEINS--MTKMYNFISCDLLNASETK 77
Query: 71 QNLSQIRH-VTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQTGI 130
Q LS ++ V+H+FWVTW+ + D+ +C QN+ ML NALDA+LP A L+H SLQTG+
Sbjct: 78 QRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSLQTGM 137
Query: 131 KHYVSLTSAAVT-GGGRGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRVAWS 190
KHYVSL + G G Y EE PR +SG NFYY LEDLL+E+++ V WS
Sbjct: 138 KHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKITRSS-----VVWS 197
Query: 191 VLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDARLVAE 250
V RPGL++G S+RT +NFMGSLCVYGA+CK L PFVFGGT CW E IDGSD+ LVAE
Sbjct: 198 VQRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLVAE 257
Query: 251 QHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEMFSPEFNYTAAM 310
QH++AAT +GEAFNA NG F WKEIW + +KLGV MF F + M
Sbjct: 258 QHIFAATSG-KVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQVNETTMFDEGFWFGREM 317
Query: 311 GDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFTLKYKMLD 370
++ VW EIV +E L TE+E+LANW +D+LFR P K++ R K DRFGF KY+ LD
Sbjct: 318 VERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYRTLD 377
Query: 371 SIFYWIDLMRRDNLIP 385
S+ YWID+MR + LIP
Sbjct: 378 SVLYWIDVMRDEKLIP 385
BLAST of Sed0016701 vs. TAIR 10
Match:
AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )
HSP 1 Score: 234.2 bits (596), Expect = 1.8e-61
Identity = 146/377 (38.73%), Postives = 202/377 (53.58%), Query Frame = 0
Query: 11 VAVIFGVTGMVGKELAKTL-LGSTAG--WKVYGVARRPDNVSPIADPNFHFIACDLLDRH 70
VA+I GVTG+VG LA+ L L T G WKVYGVARRP + AD +I CD+ D
Sbjct: 28 VALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSDAE 87
Query: 71 VAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQT 130
LS + VTH+F+VTW + +S++C + N +ML N L A++P AP+LRHV LQT
Sbjct: 88 DTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCLQT 147
Query: 131 GIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRVAW 190
G KHY L G R + + E+ PR NFYY ED+L E + + V W
Sbjct: 148 GTKHY--LGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK----KIETVTW 207
Query: 191 SVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDARLVA 250
S+ RP +I G S + N +G+LCVY AICK P +F G+ W E + SDA L+A
Sbjct: 208 SIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAW-EGFMTASDADLIA 267
Query: 251 EQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEMFSPEFNYTAA 310
EQ +WAA ++ + EAFN N F+WK +W +AE+ G+ E
Sbjct: 268 EQQIWAA---VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE-GKNLGLVEM 327
Query: 311 MGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFTLKYKML 370
M K VW E+V+E L ++EE+ W D + + G M+ S +KS +GF
Sbjct: 328 MKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEG-MIDSMNKSKEYGFLGFRNSN 387
Query: 371 DSIFYWIDLMRRDNLIP 385
+S WID + ++P
Sbjct: 388 NSFISWIDKYKAFKIVP 388
BLAST of Sed0016701 vs. TAIR 10
Match:
AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )
HSP 1 Score: 234.2 bits (596), Expect = 1.8e-61
Identity = 146/377 (38.73%), Postives = 202/377 (53.58%), Query Frame = 0
Query: 11 VAVIFGVTGMVGKELAKTL-LGSTAG--WKVYGVARRPDNVSPIADPNFHFIACDLLDRH 70
VA+I GVTG+VG LA+ L L T G WKVYGVARRP + AD +I CD+ D
Sbjct: 27 VALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSDAE 86
Query: 71 VAHQNLSQIRHVTHLFWVTWAAQLRFDSQDCNDQNRAMLANALDAVLPTAPDLRHVSLQT 130
LS + VTH+F+VTW + +S++C + N +ML N L A++P AP+LRHV LQT
Sbjct: 87 DTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCLQT 146
Query: 131 GIKHYVSLTSAAVTGGGRGEVVYCEESPRANSGSNFYYALEDLLRERLSGGGGGGKRVAW 190
G KHY L G R + + E+ PR NFYY ED+L E + + V W
Sbjct: 147 GTKHY--LGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK----KIETVTW 206
Query: 191 SVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGTAACWSEVCIDGSDARLVA 250
S+ RP +I G S + N +G+LCVY AICK P +F G+ W E + SDA L+A
Sbjct: 207 SIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAW-EGFMTASDADLIA 266
Query: 251 EQHVWAATKQINGSGEGEAFNACNGWSFRWKEIWGAVAEKLGVAAAAAEMFSPEFNYTAA 310
EQ +WAA ++ + EAFN N F+WK +W +AE+ G+ E
Sbjct: 267 EQQIWAA---VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE-GKNLGLVEM 326
Query: 311 MGDKGEVWAEIVEEEGLFPTEMEELANWGLMDSLFRLPGKMVASRHKSDRFGFTLKYKML 370
M K VW E+V+E L ++EE+ W D + + G M+ S +KS +GF
Sbjct: 327 MKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEG-MIDSMNKSKEYGFLGFRNSN 386
Query: 371 DSIFYWIDLMRRDNLIP 385
+S WID + ++P
Sbjct: 387 NSFISWIDKYKAFKIVP 387
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022998181.1 | 4.2e-182 | 80.76 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima] | [more] |
XP_022948773.1 | 1.0e-180 | 80.15 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata] | [more] |
KAG6607304.1 | 4.0e-180 | 80.15 | (S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. ... | [more] |
KAG7036983.1 | 6.8e-180 | 80.15 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp... | [more] |
XP_038895390.1 | 2.6e-179 | 80.10 | (S)-8-oxocitronellyl enol synthase CYC2 [Benincasa hispida] | [more] |
Match Name | E-value | Identity | Description | |
A0A1C9CX66 | 6.4e-64 | 40.42 | (S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC... | [more] |
Q9STX2 | 2.5e-60 | 38.73 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP... | [more] |
A0A221J5X1 | 1.2e-57 | 36.75 | (S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 ... | [more] |
A0A1C9CX56 | 2.0e-57 | 37.60 | (S)-8-oxocitronellyl enol synthase CYC1 OS=Camptotheca acuminata OX=16922 GN=CYC... | [more] |
Q6PQJ9 | 3.4e-57 | 38.20 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1K9J8 | 2.1e-182 | 80.76 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO... | [more] |
A0A6J1GA93 | 5.1e-181 | 80.15 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=... | [more] |
A0A0A0LVI2 | 4.1e-175 | 77.75 | Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 P... | [more] |
A0A6J1HM54 | 8.6e-173 | 79.37 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=... | [more] |
A0A5D3BGL7 | 2.5e-172 | 77.33 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo var. makuwa... | [more] |
Match Name | E-value | Identity | Description | |
AT5G58750.1 | 6.0e-110 | 53.19 | NAD(P)-binding Rossmann-fold superfamily protein | [more] |
AT4G24220.1 | 1.8e-61 | 38.73 | NAD(P)-binding Rossmann-fold superfamily protein | [more] |
AT4G24220.2 | 1.8e-61 | 38.73 | NAD(P)-binding Rossmann-fold superfamily protein | [more] |