Sed0014598 (gene) Chayote v1

Overview
NameSed0014598
Typegene
OrganismSechium edule (Chayote v1)
DescriptionCadmium/zinc-transporting ATPase HMA2
LocationLG11: 6105915 .. 6110454 (+)
RNA-Seq ExpressionSed0014598
SyntenySed0014598
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGAGCTTGTAAAAGAAGCTCAAAATAACAAATCCAAAACTCAGAGATTCATTGAAGAATGTGCAAAATATTACACCCCAGGTAAGTTTAGCAAAAATTATACTTCTTTTTTCAAATAATTTGAGATTCCAATTGGTCCCTTTTTAGAGTTTTAAAAATAGTTCATATGGGTCTTGAAAATAGTTCAAATAGTTTCTACATTTTCCATATATAAAATTTGAGAAGGCGAGACAAAATTTCAAAATCTTGACTTTCTTTTAATATAACTTTTAAATCTAAAATCATATGATTTTAGATTTGAATTGAAATTGTACATAAAGTTGCATGGTGTGTATGGCTCAAGCATAATTGCACTAGAATATTCAACAAGGAAAAAGGGCAAGGCATATGGGAAGATATCATTTCCAATGCAACTCTATGGTGTAAAATTTAACTATTTTAAGAATTATGAATTTTTTTTCTTTTTTTTGTTTCAAAAAGAAAAACTAAAGGTCTGTTTAATTTAAGATAATCAGGATAAATGAAATCATTTAATACCCGAAATCTTAGATATGCGAAATCATAGATGTTTGACTTTCTGGATGACTATGTTTGTTTTGTAAGATTTTTTTAATGAAAATTTGGTAATATTTAGATAATTGTGTTTCTTTTGTAGAAATCGTACTTGACATGACGTAAATTTACCTAATATTCCAAGAATGCTCATATAATATTCATATATTTTTGCATTAAAATAAATTTAGTTTATACTCATAATTATTTATATTAAATTTTAATTTCATGCCTAAATGAATTATAAATTTTGAAAATAATTGTATAGTTAATGGTACTTTTTAATGGTTCTAAAAAAAATGGTACTTTTTAATGACTTTTATATATATACCTATATAATCAACATAAATAAAATTTTGAAAGAAATAGTATCAACATTGTAATTTTTTTTAAATGTAACCATCTTTGATAATCTAAAATAAAAAACAATTCAAATTATATACGTATATGTATGTATGTGCATATGTATATTTTAAAATAGATAAGTAAAGAAAATTGATAAATATAAATATATTTTTTTAAAAAAAAGAAGGGCAAAATCGTCAATAGAAGATTCCCTTATCGTGAAAATCTTGCAAACTCACCAATCAAAAGGGCATCTAGATCTTGTCCATGCCCTTCATATGGGAATTTTAGGATTGTCGAAAATTTCACGGTACCCAATATTCTTCGATCACTACGAAACGAACATGGTAATGATACCCATTTCATAGGAGTTTCCGATTATCTAAACAATTTAAGGGGACAATTTTCCCCTCGCATTCCATTTTAGTCGTGCTTGTTGAAAATCTTTTTACTTTTAAAGATAATAATACAGTCTATGTAATTAAAGTTTTGAATAATTGAATCTCAACTCCTAAAGTCTGTTTATTGAAACTTAGAAACCAAAATAAGACTTTTTTTTATATCATCCCTTCTAATCATATTTCATCTTCTTTTAGGATCAAGATTAATTCAAAACCATATCATCTTCAAAATAAAGTAATAATGATAAGTATATCTATATCTATACACGTATATCTATATATATATATTCACATGGCATTTAAACTAAAAGACTTTCCTTCTTTTCTTCATGTTTTTGTGCAGTTGTTCTTGTCATATCAGCTTGTCTTGCAGCCATTCCAGCTGCAATGAGAGTTCACAATCTCAGCCATTGGTTTCACTTAGCTCTTGTTGTTTTAGTTAGTGCATTTCCCTGCGCCCTAATCCTTTCCGCCCCGATCGCCGCATTTTTCGCAGTCACGAAGGCAGCCATGGCTGGAGTTCTAATCAAAGGCGGCAACCACCTTGAAGTTCTTGCAAAGATTAAGGTTATGGCATTCGACAAGACTGGCACAATCACTCGAGGTGAATTTGTGGTTAGAGATATTCGAACACTACAAGATGATATCAGCTTGCACACCTTGCTTCATTGGTAAAATTACACAAACACTTCTCTATTTATTTCTTCATGTTTGCTATAGTTTTCAATTCTTATCTCTAATCTTTTGAAGTTTTCATTTTGATCATGTAGTTGTAACTAAATGACTAAAAAACATGACAGGTAAATTCATTAAAGAATTCATTAGTTCCTGATATAGTAATAAGCTTCACGAGTTTATTTTGAGTAATTGATCTGATTTGCAAGTTTGTTAGGAATACAAACAAAGTATAATTCTACGTTTGTTAAATAAGGGAGGAAAAACATTTTCATTCACATGATGCCTTTTAAGTGAACAAAAAACAGAGTGATGAGAGTTTAGGCCCAAAATGGACAATATCATACCAATGTAGAGATACGGGTAGGTTTTTCATCCTTTATCAAGTGGTATCAAAACGGTTTTTCCTAACTTAGTCATATCGGAATAACCTTAAGTATGAAACAAAGATTTGTGAGTCTCAAGATGTGATGTGAAATAGTCAAGATGAAAATCTGTGGTTGAGTCTCCCAATGTGTGCTCCAGTGCGAGTGCTTCTGAATATGCTCGTGATTGAATATCTGTTCACTCCAGAGTGGGAGTGCTTCTGAAACAAGTTTTATAGTGAGGAGTGCTTTTGAAATGGTTGCCTTAGTAAGGGGTAGCTACTGTGCACTCAAACATAAATCGAGTAGATATGTGGTTGGGCTTTTCGGTTTTCTCAAGTGTAATTCGAAAGGAATCATGGTTTGAGTTAAGGACGTGGTCTCAAATATAATTAAAAAAATGACTATATATAAGGGAGACTCTTGCATGCGGCACTTTGTTCGATGAGAGGATTGTTGGGAATACAAATAAAGAGTAATACCACATTGGTTAAATAAGGGGTAGATCATGGGCTTACAAAGGAGGACAAACGTCACCATTGGTATTAGACTTCTTGGGTGAAAAAAAAGTCATGAGAGTGCATACCCAAAGTAGACAATATCATATCAATCTGGAGATCTGGGTAGGTTTGACATGACCTATCAAAGTTTTTAATTTTACGATAAATATGTTGATTTCATTGGATAAAAACAATCTATGATATATAAATGTTTCAAATTTTTTGGTGTACTTAATGGTTTATTATTTTTTAAAGATCCTAACTTGATAATATTAAAACAACATGGGTTAGGGCATATAATTGATGGCAAATACATTCATACGATTAATAATTGACACATTTAAATGTTATAAATCTTGTGATGACAACCCACTTCTTTGGTATGCGACATTTTTGGTAAAACAAAAAAATAAAATCATAAAGATGTATGCTCAAAGTGACAATATCATAATAATTTGGAGATCTAGGCTGTTTGTATTTTGCATTACATCAAATGAATATAGCAATTTTGAACTTATTGATATGATATTATATTTCAAAAAGACAATAAGAAAAAAAGTTCGAAATGAGAGTTTATTGATTGAACTTTGTAAATAAATTAGGATTTCAAGCATTGAGAGCAAATCAAGTCATCCAATGGCAGCTGCCTTTATCAGCCATGGAAAGCTCCTTTCAGTCGATATTAAACCCAAAAACGTCGATGAATTCGAGAATTTTCCAGGAGAAGGTGTTCATGGAAAAATAGATGGGAACAATATTTACATTGGAAGTAGAAAAATTGCTCTTAGAGCTGGCTGCAAAACAGGTTTTGTTTTAATCAAACCTTTTCACCTCTTAAAACTTTTATCCAAAAATCTCTTGAAAATGTTATCCAAACATGTTTTACTCAGTTCCGAACTTAGACAAGGAAATGAAACAAGGGCAGACTGTCGGATATGTATTTTGTGGGGCAACGCTAGCCGGATCCTTCAGTCTTTCGGATGCGTGTCGATCGGGAGTTAAAGAGGGGATTGAGGAAATTAAGAGTTTTGGTATAAAAACAGCCATGCTTACTGGAGATTGTAGGGCAGCAGCCATGCATGCCCAACAAAAGGTTTGCTTCATATTTTTCGTTTATCACGAATCGAACTCTTGAAATTTGATTTGCAAGTCTTACATGAATCTTTTAATGATGCATATTATCTACAGCTAGGGAACGCGCTCGATGTAATCCATTTCGAACTTCTACCGAAGGAGAAGGCACGTATCGTAAAAGAATTTAAAAGGGATGGACCAACTGCCATGGTTGGAGATAGTTCAAATGATACCCCAGCATTTGCCACAGCTGATATTGGTATAACAATGGGGGTTTCAAGCTCAGCATTAGCCAGTGAGACTGGGAATGTAATCCTAACATCAAATGACATGAGAAAAATTCCAAAAGCCATCAGACTAGCAAAGAAATGCCATGCAAAAGTAGTTCAAAATGTCATTTTGTCGATAGGTACGAAGACCGCAATCATCGGCTTGGCAATCGGTGGACATCCGCTCATCTGGGCGGCCGTCCTCACCGATGTCGGTACCTGCCTATTGGTCATCTTAAACAGCATGCTTCTTTTAAGAGAAACTAAGCATAAACATGGAAAAAAATCTTTCAAACCATATTCCACAAAACATGTTACTATTGACATTCATGATGGTAATACTGTGAGGGAATAG

mRNA sequence

ATGGGTGAGCTTGTAAAAGAAGCTCAAAATAACAAATCCAAAACTCAGAGATTCATTGAAGAATGTGCAAAATATTACACCCCAGTTGTTCTTGTCATATCAGCTTGTCTTGCAGCCATTCCAGCTGCAATGAGAGTTCACAATCTCAGCCATTGGTTTCACTTAGCTCTTGTTGTTTTAGTTAGTGCATTTCCCTGCGCCCTAATCCTTTCCGCCCCGATCGCCGCATTTTTCGCAGTCACGAAGGCAGCCATGGCTGGAGTTCTAATCAAAGGCGGCAACCACCTTGAAGTTCTTGCAAAGATTAAGGTTATGGCATTCGACAAGACTGGCACAATCACTCGAGGTGAATTTGTGGTTAGAGATATTCGAACACTACAAGATGATATCAGCTTGCACACCTTGCTTCATTGGATTTCAAGCATTGAGAGCAAATCAAGTCATCCAATGGCAGCTGCCTTTATCAGCCATGGAAAGCTCCTTTCAGTCGATATTAAACCCAAAAACGTCGATGAATTCGAGAATTTTCCAGGAGAAGGTGTTCATGGAAAAATAGATGGGAACAATATTTACATTGGAAGTAGAAAAATTGCTCTTAGAGCTGGCTGCAAAACAGTTCCGAACTTAGACAAGGAAATGAAACAAGGGCAGACTGTCGGATATGTATTTTGTGGGGCAACGCTAGCCGGATCCTTCAGTCTTTCGGATGCGTGTCGATCGGGAGTTAAAGAGGGGATTGAGGAAATTAAGAGTTTTGGTATAAAAACAGCCATGCTTACTGGAGATTGTAGGGCAGCAGCCATGCATGCCCAACAAAAGCTAGGGAACGCGCTCGATGTAATCCATTTCGAACTTCTACCGAAGGAGAAGGCACGTATCGTAAAAGAATTTAAAAGGGATGGACCAACTGCCATGGTTGGAGATAGTTCAAATGATACCCCAGCATTTGCCACAGCTGATATTGGTATAACAATGGGGGTTTCAAGCTCAGCATTAGCCAGTGAGACTGGGAATGTAATCCTAACATCAAATGACATGAGAAAAATTCCAAAAGCCATCAGACTAGCAAAGAAATGCCATGCAAAAGTAGTTCAAAATGTCATTTTGTCGATAGGTACGAAGACCGCAATCATCGGCTTGGCAATCGGTGGACATCCGCTCATCTGGGCGGCCGTCCTCACCGATGTCGGTACCTGCCTATTGGTCATCTTAAACAGCATGCTTCTTTTAAGAGAAACTAAGCATAAACATGGAAAAAAATCTTTCAAACCATATTCCACAAAACATGTTACTATTGACATTCATGATGGTAATACTGTGAGGGAATAG

Coding sequence (CDS)

ATGGGTGAGCTTGTAAAAGAAGCTCAAAATAACAAATCCAAAACTCAGAGATTCATTGAAGAATGTGCAAAATATTACACCCCAGTTGTTCTTGTCATATCAGCTTGTCTTGCAGCCATTCCAGCTGCAATGAGAGTTCACAATCTCAGCCATTGGTTTCACTTAGCTCTTGTTGTTTTAGTTAGTGCATTTCCCTGCGCCCTAATCCTTTCCGCCCCGATCGCCGCATTTTTCGCAGTCACGAAGGCAGCCATGGCTGGAGTTCTAATCAAAGGCGGCAACCACCTTGAAGTTCTTGCAAAGATTAAGGTTATGGCATTCGACAAGACTGGCACAATCACTCGAGGTGAATTTGTGGTTAGAGATATTCGAACACTACAAGATGATATCAGCTTGCACACCTTGCTTCATTGGATTTCAAGCATTGAGAGCAAATCAAGTCATCCAATGGCAGCTGCCTTTATCAGCCATGGAAAGCTCCTTTCAGTCGATATTAAACCCAAAAACGTCGATGAATTCGAGAATTTTCCAGGAGAAGGTGTTCATGGAAAAATAGATGGGAACAATATTTACATTGGAAGTAGAAAAATTGCTCTTAGAGCTGGCTGCAAAACAGTTCCGAACTTAGACAAGGAAATGAAACAAGGGCAGACTGTCGGATATGTATTTTGTGGGGCAACGCTAGCCGGATCCTTCAGTCTTTCGGATGCGTGTCGATCGGGAGTTAAAGAGGGGATTGAGGAAATTAAGAGTTTTGGTATAAAAACAGCCATGCTTACTGGAGATTGTAGGGCAGCAGCCATGCATGCCCAACAAAAGCTAGGGAACGCGCTCGATGTAATCCATTTCGAACTTCTACCGAAGGAGAAGGCACGTATCGTAAAAGAATTTAAAAGGGATGGACCAACTGCCATGGTTGGAGATAGTTCAAATGATACCCCAGCATTTGCCACAGCTGATATTGGTATAACAATGGGGGTTTCAAGCTCAGCATTAGCCAGTGAGACTGGGAATGTAATCCTAACATCAAATGACATGAGAAAAATTCCAAAAGCCATCAGACTAGCAAAGAAATGCCATGCAAAAGTAGTTCAAAATGTCATTTTGTCGATAGGTACGAAGACCGCAATCATCGGCTTGGCAATCGGTGGACATCCGCTCATCTGGGCGGCCGTCCTCACCGATGTCGGTACCTGCCTATTGGTCATCTTAAACAGCATGCTTCTTTTAAGAGAAACTAAGCATAAACATGGAAAAAAATCTTTCAAACCATATTCCACAAAACATGTTACTATTGACATTCATGATGGTAATACTGTGAGGGAATAG

Protein sequence

MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVVRDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEGVHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRSGVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFKRDGPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKKCHAKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRETKHKHGKKSFKPYSTKHVTIDIHDGNTVRE
Homology
BLAST of Sed0014598 vs. NCBI nr
Match: XP_022153334.1 (putative inactive cadmium/zinc-transporting ATPase HMA3 [Momordica charantia])

HSP 1 Score: 674.5 bits (1739), Expect = 6.3e-190
Identity = 348/433 (80.37%), Postives = 382/433 (88.22%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M ELV+EAQN+KSKTQRFI+ECAKYYTP V+VISAC+AAIPAA+RVHNLSHW HLALVVL
Sbjct: 290 MAELVEEAQNSKSKTQRFIDECAKYYTPAVVVISACVAAIPAALRVHNLSHWLHLALVVL 349

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VSA PCALILS P+AAF A+TKAA AGVLIKGG+HLE+LAKIKVMAFDKTGTITRGEFVV
Sbjct: 350 VSACPCALILSTPVAAFCALTKAATAGVLIKGGDHLEILAKIKVMAFDKTGTITRGEFVV 409

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
            D R L+DDISLHTLLHW+S IESKSSHPMAAA ++HG+LLSV I P+NV+EFENFPGEG
Sbjct: 410 TDFRALRDDISLHTLLHWVSCIESKSSHPMAAALVNHGRLLSVHINPENVEEFENFPGEG 469

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRS 240
           V GKIDGN+IYIGSRKIA RAGC TVP+LD E KQG TVGY+F GAT+AGSFSLSDACRS
Sbjct: 470 VRGKIDGNDIYIGSRKIAARAGCTTVPSLDDETKQGLTVGYIFRGATIAGSFSLSDACRS 529

Query: 241 GVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFKRD 300
           GV+E I+EIKSFGIKTAMLTGDC AAA HAQ++LG+ LDVIH ELLPKEKA I+KE KRD
Sbjct: 530 GVREAIKEIKSFGIKTAMLTGDCSAAAAHAQEQLGDVLDVIHSELLPKEKANIIKELKRD 589

Query: 301 GPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKKCH 360
           GPTAMVGD  NDTPA ATADIGI+MGVS SALA+ETGNVIL SNDMRKIPKAI+LAKK H
Sbjct: 590 GPTAMVGDGLNDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSH 649

Query: 361 AKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLL----RETKHK 420
           AKVVQNVILSIGTKTAI+ LA  GHPL+WAAVL DVGTC++VILNSMLLL    R T   
Sbjct: 650 AKVVQNVILSIGTKTAILALAFAGHPLVWAAVLADVGTCVVVILNSMLLLRGADRHTNVN 709

Query: 421 HGKKSFKPYSTKH 430
              KS KP STKH
Sbjct: 710 KCCKSAKPCSTKH 722

BLAST of Sed0014598 vs. NCBI nr
Match: XP_038891185.1 (LOW QUALITY PROTEIN: putative inactive cadmium/zinc-transporting ATPase HMA3 [Benincasa hispida])

HSP 1 Score: 667.2 bits (1720), Expect = 1.0e-187
Identity = 343/433 (79.21%), Postives = 378/433 (87.30%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M ELV+EAQNNKSKTQRFI+ECAKYYTP V++ISACLAAIPAA+RVHNLSHW HLALVVL
Sbjct: 298 MAELVEEAQNNKSKTQRFIDECAKYYTPAVVIISACLAAIPAALRVHNLSHWLHLALVVL 357

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VSA PCALILS P+AAF A+TKAAMAGVLIKGG+HLEVLAK+KVMAFDKTGTITRGEFVV
Sbjct: 358 VSACPCALILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFVV 417

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
              + L+DDIS HTLLHW+SSIESKSSHPMAAA ++HGKLLS+D+KPKNV+EFENFPGEG
Sbjct: 418 THFQALRDDISFHTLLHWVSSIESKSSHPMAAALVNHGKLLSIDLKPKNVEEFENFPGEG 477

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRS 240
           V GKIDGN+IYIGSRKIA+RAG     + D E KQ QT+GYVFCG T+ GSF LSD+CRS
Sbjct: 478 VRGKIDGNDIYIGSRKIAVRAGYTPDLSFDDETKQEQTLGYVFCGGTIIGSFGLSDSCRS 537

Query: 241 GVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFKRD 300
           GVKE IEEIKSFGIKTAMLTGDC AAAMHAQ++LGNALD+IH ELLPKEKA I+K+FK D
Sbjct: 538 GVKEAIEEIKSFGIKTAMLTGDCNAAAMHAQEQLGNALDIIHSELLPKEKANIIKDFKED 597

Query: 301 GP-TAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKKC 360
           G   AMVGD  NDTPA  TADIGI+MGVS SALA+ETGNVIL SNDMRKIPK I+LAKK 
Sbjct: 598 GAIIAMVGDGLNDTPALVTADIGISMGVSGSALATETGNVILMSNDMRKIPKTIKLAKKF 657

Query: 361 HAKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRETKHKHGK 420
           H KVVQNVILSI TKTAI+GLA  GHPL+WAAVL DVGTCLLVILNSMLLLR   HKHG+
Sbjct: 658 HKKVVQNVILSISTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGINHKHGE 717

Query: 421 ---KSFKPYSTKH 430
              KS KP  TK+
Sbjct: 718 KCCKSSKPCLTKY 730

BLAST of Sed0014598 vs. NCBI nr
Match: XP_023545589.1 (putative inactive cadmium/zinc-transporting ATPase HMA3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 657.9 bits (1696), Expect = 6.1e-185
Identity = 337/437 (77.12%), Postives = 378/437 (86.50%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M E V+EAQNNKSKTQRFI+ECAKYYTP V+VISAC+AAIPAA+RVHNL+HWFHLALVVL
Sbjct: 297 MAEFVEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAALRVHNLTHWFHLALVVL 356

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VSA PCALILS P+AAF A+TKAAMAGVLIKG +HLEVLAKIKV+AFDKTGTITRGEFVV
Sbjct: 357 VSACPCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVV 416

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
            +   L+DDIS HTLLHW+SSIESKSSHPMAAA ++H KLLS+DIKP+ V+EFENFPGEG
Sbjct: 417 TNFEALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEG 476

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRS 240
           V GKIDG +IYIGS KIA RAGC +   L++EMKQGQT+GYVFCG    GSF LSD+CRS
Sbjct: 477 VRGKIDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCGEMAVGSFGLSDSCRS 536

Query: 241 GVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFKRD 300
           G KEG+EE+KSFGIKTAMLTGDC AAAMHAQQ+L NA+DVIH ELLPKEKA ++KEFKRD
Sbjct: 537 GAKEGMEELKSFGIKTAMLTGDCNAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRD 596

Query: 301 -GPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKKC 360
            G TAMVGD  NDTPA ATADIGI+MG+S S LA++TGNVIL SND+ KIP+AI+LA+K 
Sbjct: 597 YGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKS 656

Query: 361 HAKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRETKHKHGK 420
           HAKVVQNVILSI TK AI+GLAI GHPL+WAAVL DVGTCLLVILNSMLLLR + HKHG 
Sbjct: 657 HAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGN 716

Query: 421 ---KSFKPYSTKHVTID 434
              KS K  STKH   D
Sbjct: 717 KCCKSSKLCSTKHGRCD 733

BLAST of Sed0014598 vs. NCBI nr
Match: KAG6598888.1 (Cadmium/zinc-transporting ATPase HMA2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 654.4 bits (1687), Expect = 6.8e-184
Identity = 337/437 (77.12%), Postives = 376/437 (86.04%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M E V+EAQNNKSKTQRFI+ECAKYYTP V+VISAC+AAIPAA RVHNLSHWFHLALVVL
Sbjct: 297 MAEFVEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVL 356

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VSA PCALILS P+AAF A+TKAAMAGVLIKG +HLEVLAKIKV+AFDKTGTITRGEFVV
Sbjct: 357 VSACPCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVV 416

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
            D + L+DDIS HTLLHW+SSIESKSSHPMAAA ++H KLLS+DIKP+ V+EFENFPGEG
Sbjct: 417 TDFQALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEG 476

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRS 240
           V GKIDG +IYIGS KIA RAGC +   L++EMKQGQT+GYVFC     GSF LSD+CRS
Sbjct: 477 VRGKIDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRS 536

Query: 241 GVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFKRD 300
           G KEG+EE+KS GIKTAMLTGDC AAAMHAQQ+L NA+DVIH ELLPKEKA ++KEFKRD
Sbjct: 537 GAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRD 596

Query: 301 -GPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKKC 360
            G TAMVGD  NDTPA ATADIGI+MG+S S LA++TGNVIL SND+ KIP+AI+LA+K 
Sbjct: 597 YGGTAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKS 656

Query: 361 HAKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRETKHKHGK 420
           HAKVVQNVILSI TK AI+GLAI GHPL+WAAVL DVGTCLLVILNSMLLLR + HKHG 
Sbjct: 657 HAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGN 716

Query: 421 ---KSFKPYSTKHVTID 434
              KS K  STKH   D
Sbjct: 717 KCCKSSKLCSTKHGRCD 733

BLAST of Sed0014598 vs. NCBI nr
Match: XP_022929619.1 (cadmium/zinc-transporting ATPase HMA2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 653.7 bits (1685), Expect = 1.2e-183
Identity = 337/437 (77.12%), Postives = 375/437 (85.81%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M E V+EAQNNKSKTQRFI+ECAKYYTP V+VISAC+AAIPAA RVHNLSHWFHLALVVL
Sbjct: 297 MAEFVEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVL 356

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VSA PCALILS P+AAF A+TKAAMAGVLIKG +HLEVLAKIKV+AFDKTGTITRGEFVV
Sbjct: 357 VSACPCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVV 416

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
            D + L+DDIS HTLLHW+SSIESKSSHPMAAA ++H KLLS DIKP+ V+EFENFPGEG
Sbjct: 417 TDFQALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEG 476

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRS 240
           V GKIDG +IYIGS KIA RAGC +   L++EMKQGQT+GYVFC     GSF LSD+CRS
Sbjct: 477 VRGKIDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRS 536

Query: 241 GVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFKRD 300
           G KEG+EE+KS GIKTAMLTGDC AAAMHAQQ+L NA+DVIH ELLPKEKA ++KEFKRD
Sbjct: 537 GAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRD 596

Query: 301 -GPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKKC 360
            G TAMVGD  NDTPA ATADIGI+MG+S S LA++TGNVIL SND+ KIP+AI+LA+K 
Sbjct: 597 YGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKS 656

Query: 361 HAKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRETKHKHGK 420
           HAKVVQNVILSI TK AI+GLAI GHPL+WAAVL DVGTCLLVILNSMLLLR + HKHG 
Sbjct: 657 HAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGN 716

Query: 421 ---KSFKPYSTKHVTID 434
              KS K  STKH   D
Sbjct: 717 KCCKSSKLCSTKHGRCD 733

BLAST of Sed0014598 vs. ExPASy Swiss-Prot
Match: Q9SZW4 (Cadmium/zinc-transporting ATPase HMA2 OS=Arabidopsis thaliana OX=3702 GN=HMA2 PE=2 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 2.3e-158
Identity = 276/433 (63.74%), Postives = 359/433 (82.91%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M +LV+EAQN+K++TQRFI++C+KYYTP +++IS C  AIP A++VHNL HW HLALVVL
Sbjct: 284 MAKLVEEAQNSKTETQRFIDKCSKYYTPAIILISICFVAIPFALKVHNLKHWVHLALVVL 343

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VSA PC LILS P+A F A+TKAA +G+LIKG ++LE LAKIK++AFDKTGTITRGEF+V
Sbjct: 344 VSACPCGLILSTPVATFCALTKAATSGLLIKGADYLETLAKIKIVAFDKTGTITRGEFIV 403

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
            D ++L +DISL +LL+W+SS ESKSSHPMAAA + + + +SV+ KP+ V++++NFPGEG
Sbjct: 404 MDFQSLSEDISLQSLLYWVSSTESKSSHPMAAAVVDYARSVSVEPKPEAVEDYQNFPGEG 463

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRS 240
           ++GKIDG  +YIG+++IA RAGC +VP++D + K G+T+GYV+ G TLAG F+LSDACRS
Sbjct: 464 IYGKIDGKEVYIGNKRIASRAGCLSVPDIDVDTKGGKTIGYVYVGETLAGVFNLSDACRS 523

Query: 241 GVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFKR- 300
           GV + ++E+KS GIK AMLTGD  AAAMHAQ++LGNA+D++  ELLP++K+ I+K+ KR 
Sbjct: 524 GVAQAMKELKSLGIKIAMLTGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKRE 583

Query: 301 DGPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKKC 360
           +GPTAMVGD  ND PA ATADIGI+MGVS SALA+ETGN+IL SND+R+IP+AI+LAK+ 
Sbjct: 584 EGPTAMVGDGLNDAPALATADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRA 643

Query: 361 HAKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRETKHKHGK 420
             KVV+NV++SI  K AI+ LA  GHPLIWAAVL DVGTCLLVILNSMLLL + KHK G 
Sbjct: 644 KRKVVENVVISITMKGAILALAFAGHPLIWAAVLADVGTCLLVILNSMLLLSD-KHKTGN 703

Query: 421 KSFKPYSTKHVTI 433
           K ++  S+  V I
Sbjct: 704 KCYRESSSSSVLI 715

BLAST of Sed0014598 vs. ExPASy Swiss-Prot
Match: O64474 (Putative cadmium/zinc-transporting ATPase HMA4 OS=Arabidopsis thaliana OX=3702 GN=HMA4 PE=1 SV=2)

HSP 1 Score: 554.3 bits (1427), Expect = 1.3e-156
Identity = 265/428 (61.92%), Postives = 355/428 (82.94%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M +LV+EAQ++K+K+QR I++C++YYTP ++++SAC+A +P  M+VHNL HWFHLALVVL
Sbjct: 294 MAKLVEEAQSSKTKSQRLIDKCSQYYTPAIILVSACVAIVPVIMKVHNLKHWFHLALVVL 353

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VS  PC LILS P+A F A+TKAA +G+LIK  ++L+ L+KIK++AFDKTGTITRGEF+V
Sbjct: 354 VSGCPCGLILSTPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKTGTITRGEFIV 413

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
            D ++L  DI+L +LL+W+SS+ESKSSHPMAA  + + K +SV+ +P+ V++++NFPGEG
Sbjct: 414 IDFKSLSRDINLRSLLYWVSSVESKSSHPMAATIVDYAKSVSVEPRPEEVEDYQNFPGEG 473

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRS 240
           ++GKIDGN+I+IG++KIA RAGC TVP ++ + K G+TVGYV+ G  LAG F+LSDACRS
Sbjct: 474 IYGKIDGNDIFIGNKKIASRAGCSTVPEIEVDTKGGKTVGYVYVGERLAGFFNLSDACRS 533

Query: 241 GVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFKRD 300
           GV + + E+KS GIKTAMLTGD +AAAMHAQ++LGN LDV+H +LLP++K+RI++EFK++
Sbjct: 534 GVSQAMAELKSLGIKTAMLTGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKE 593

Query: 301 GPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKKCH 360
           GPTAMVGD  ND PA ATADIGI+MG+S SALA++TGN+IL SND+R+IP+A++LA++  
Sbjct: 594 GPTAMVGDGVNDAPALATADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRAR 653

Query: 361 AKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRETKHKHGKK 420
            KVV+NV LSI  K  I+ LA  GHPLIWAAVL DVGTCLLVI NSMLLLRE K    KK
Sbjct: 654 RKVVENVCLSIILKAGILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLREKKKIGNKK 713

Query: 421 SFKPYSTK 429
            ++  ++K
Sbjct: 714 CYRASTSK 721

BLAST of Sed0014598 vs. ExPASy Swiss-Prot
Match: P0CW78 (Cadmium/zinc-transporting ATPase HMA3 OS=Arabidopsis thaliana OX=3702 GN=HMA3 PE=1 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 4.2e-144
Identity = 255/416 (61.30%), Postives = 333/416 (80.05%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M +LV+EAQ +++KTQRFI++C++YYTP V+V +AC A IP  ++V +LSHWFHLALVVL
Sbjct: 290 MTKLVEEAQKSQTKTQRFIDKCSRYYTPAVVVSAACFAVIPVLLKVQDLSHWFHLALVVL 349

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VS  PC LILS P+A F A+TKAA +G LIK G+ LE LAKIK++AFDKTGTIT+ EF+V
Sbjct: 350 VSGCPCGLILSTPVATFCALTKAATSGFLIKTGDCLETLAKIKIVAFDKTGTITKAEFMV 409

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
            D R+L   I+LH LL+W+SSIE KSSHPMAAA I +   +SV+ KP  V+ F+NFPGEG
Sbjct: 410 SDFRSLSPSINLHKLLNWVSSIECKSSHPMAAALIDYAISVSVEPKPDIVENFQNFPGEG 469

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKT--VPNLDKEMKQGQTVGYVFCGATLAGSFSLSDAC 240
           V+G+IDG +IYIG+++IA RAGC T  VP+++  MK+G+T+GY++ GA L GSF+L D C
Sbjct: 470 VYGRIDGQDIYIGNKRIAQRAGCLTDNVPDIEATMKRGKTIGYIYMGAKLTGSFNLLDGC 529

Query: 241 RSGVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFK 300
           R GV + ++E+KS GI+TAMLTGD + AAM  Q++L NALD++H ELLP++KARI+ +FK
Sbjct: 530 RYGVAQALKELKSLGIQTAMLTGDNQDAAMSTQEQLENALDIVHSELLPQDKARIIDDFK 589

Query: 301 RDGPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKK 360
             GPT MVGD  ND PA A ADIGI+MG+S SALA+ETG++IL SND+RKIPK +RLAK+
Sbjct: 590 IQGPTMMVGDGLNDAPALAKADIGISMGISGSALATETGDIILMSNDIRKIPKGMRLAKR 649

Query: 361 CHAKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRETK 415
            H KV++NV+LS+  K AI+ L   G+PL+WAAVL D GTCLLVILNSM+LLR+ +
Sbjct: 650 SHKKVIENVVLSVSIKGAIMVLGFVGYPLVWAAVLADAGTCLLVILNSMILLRDER 705

BLAST of Sed0014598 vs. ExPASy Swiss-Prot
Match: A3BF39 (Cadmium/zinc-transporting ATPase HMA2 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA2 PE=1 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 5.5e-136
Identity = 249/421 (59.14%), Postives = 321/421 (76.25%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M  LV+EAQN++S TQR I+ CAKYYTP V+V++  +AAIPA  + HNL HWF LALV+L
Sbjct: 288 MARLVEEAQNSRSSTQRLIDTCAKYYTPAVVVMAGSVAAIPAIAKAHNLKHWFQLALVLL 347

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VSA PCAL+LS PIA F A+ +AA  G+LIKGG+ LE LA IKV AFDKTGTITRGEF V
Sbjct: 348 VSACPCALVLSTPIATFCALLRAARTGLLIKGGDVLESLASIKVAAFDKTGTITRGEFSV 407

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
            + + + + +SL  LL+W+SS+ES+SSHPMA+  + + +  SV+ K +NV EF+ +PGEG
Sbjct: 408 EEFQPVGERVSLQQLLYWVSSVESRSSHPMASVLVDYAQSKSVEPKSENVSEFQIYPGEG 467

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRS 240
           ++G+IDG  IYIG+++I  RA C+TVP++ K+MK G T+GYV C   L G F+LSDACR+
Sbjct: 468 IYGEIDGAGIYIGNKRILSRASCETVPDM-KDMK-GVTIGYVACNNELIGVFTLSDACRT 527

Query: 241 GVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFK-R 300
           G  E I+E++S GIK+ MLTGD  AAA +AQ +LGN L  +H ELLP++K RIV E K +
Sbjct: 528 GSAEAIKELRSLGIKSVMLTGDSSAAATYAQNQLGNILAEVHAELLPEDKVRIVGELKEK 587

Query: 301 DGPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKKC 360
           DGPT MVGD  ND PA A AD+G++MGVS SA+A ET +V L SND+R+IPKA+RLA++ 
Sbjct: 588 DGPTLMVGDGMNDAPALAKADVGVSMGVSGSAVAMETSHVALMSNDIRRIPKAVRLARRT 647

Query: 361 HAKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRETKHKHGK 420
           H  ++ N+I S+ TK AI+GLA  GHPLIWAAVL DVGTCLLVI+ SMLLLRE   +  K
Sbjct: 648 HRTIIVNIIFSVITKLAIVGLAFAGHPLIWAAVLADVGTCLLVIMYSMLLLREKDSRKAK 706

BLAST of Sed0014598 vs. ExPASy Swiss-Prot
Match: Q8H384 (Cadmium/zinc-transporting ATPase HMA3 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA3 PE=1 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.1e-112
Identity = 213/414 (51.45%), Postives = 285/414 (68.84%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M  LV+ AQN++SKTQR I+ CAKYYTP V+V++A +A IPA +    L  W+ LALV+L
Sbjct: 319 MERLVEAAQNSRSKTQRLIDSCAKYYTPAVVVVAAGVALIPALLGADGLEQWWKLALVML 378

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VSA PCAL+LS P+A+F A+ +AA  G+ IKGG+ LE L +I+ +AFDKTGTITRGEF +
Sbjct: 379 VSACPCALVLSTPVASFCAMLRAARMGIFIKGGDVLESLGEIRAVAFDKTGTITRGEFSI 438

Query: 121 RDIRTLQD-DISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGE 180
                + D  + +  LL+WI+SIESKSSHPMAAA + + +  S+   P+NV +F  +PGE
Sbjct: 439 DSFHLVGDHKVEMDHLLYWIASIESKSSHPMAAALVEYAQSKSIQPNPENVGDFRIYPGE 498

Query: 181 GVHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACR 240
           G++G+I G +IYIG+R+   RA          EM +G ++GYV C   LAG FSLSD CR
Sbjct: 499 GIYGEIHGKHIYIGNRRTLARASSPQSTQEMGEMIKGVSIGYVICDGELAGVFSLSDDCR 558

Query: 241 SGVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFK- 300
           +G  E I E+ S GIK+ MLTGD  AAA HAQ +LG  ++ +H ELLP++K R+V   K 
Sbjct: 559 TGAAEAIRELGSLGIKSVMLTGDSSAAATHAQGQLGGVMEELHSELLPEDKVRLVSGLKA 618

Query: 301 RDGPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKK 360
           R GPT MVGD  ND  A A AD+G++MG+S SA A ET +  L S+D+ ++P+A+RL + 
Sbjct: 619 RFGPTMMVGDGMNDAAALAAADVGVSMGISGSAAAMETSHATLMSSDVLRVPEAVRLGRC 678

Query: 361 CHAKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRE 413
               +  NV  S+  K A++ LA    P++WAAVL DVGTCLLV+LNSM LLRE
Sbjct: 679 ARRTIAVNVAGSVAVKAAVLALAAAWRPVLWAAVLADVGTCLLVVLNSMTLLRE 732

BLAST of Sed0014598 vs. ExPASy TrEMBL
Match: A0A6J1DIS0 (putative inactive cadmium/zinc-transporting ATPase HMA3 OS=Momordica charantia OX=3673 GN=LOC111020851 PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 3.1e-190
Identity = 348/433 (80.37%), Postives = 382/433 (88.22%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M ELV+EAQN+KSKTQRFI+ECAKYYTP V+VISAC+AAIPAA+RVHNLSHW HLALVVL
Sbjct: 290 MAELVEEAQNSKSKTQRFIDECAKYYTPAVVVISACVAAIPAALRVHNLSHWLHLALVVL 349

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VSA PCALILS P+AAF A+TKAA AGVLIKGG+HLE+LAKIKVMAFDKTGTITRGEFVV
Sbjct: 350 VSACPCALILSTPVAAFCALTKAATAGVLIKGGDHLEILAKIKVMAFDKTGTITRGEFVV 409

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
            D R L+DDISLHTLLHW+S IESKSSHPMAAA ++HG+LLSV I P+NV+EFENFPGEG
Sbjct: 410 TDFRALRDDISLHTLLHWVSCIESKSSHPMAAALVNHGRLLSVHINPENVEEFENFPGEG 469

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRS 240
           V GKIDGN+IYIGSRKIA RAGC TVP+LD E KQG TVGY+F GAT+AGSFSLSDACRS
Sbjct: 470 VRGKIDGNDIYIGSRKIAARAGCTTVPSLDDETKQGLTVGYIFRGATIAGSFSLSDACRS 529

Query: 241 GVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFKRD 300
           GV+E I+EIKSFGIKTAMLTGDC AAA HAQ++LG+ LDVIH ELLPKEKA I+KE KRD
Sbjct: 530 GVREAIKEIKSFGIKTAMLTGDCSAAAAHAQEQLGDVLDVIHSELLPKEKANIIKELKRD 589

Query: 301 GPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKKCH 360
           GPTAMVGD  NDTPA ATADIGI+MGVS SALA+ETGNVIL SNDMRKIPKAI+LAKK H
Sbjct: 590 GPTAMVGDGLNDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSH 649

Query: 361 AKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLL----RETKHK 420
           AKVVQNVILSIGTKTAI+ LA  GHPL+WAAVL DVGTC++VILNSMLLL    R T   
Sbjct: 650 AKVVQNVILSIGTKTAILALAFAGHPLVWAAVLADVGTCVVVILNSMLLLRGADRHTNVN 709

Query: 421 HGKKSFKPYSTKH 430
              KS KP STKH
Sbjct: 710 KCCKSAKPCSTKH 722

BLAST of Sed0014598 vs. ExPASy TrEMBL
Match: A0A6J1EUV8 (cadmium/zinc-transporting ATPase HMA2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436148 PE=3 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 5.6e-184
Identity = 337/437 (77.12%), Postives = 375/437 (85.81%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M E V+EAQNNKSKTQRFI+ECAKYYTP V+VISAC+AAIPAA RVHNLSHWFHLALVVL
Sbjct: 297 MAEFVEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVL 356

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VSA PCALILS P+AAF A+TKAAMAGVLIKG +HLEVLAKIKV+AFDKTGTITRGEFVV
Sbjct: 357 VSACPCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVV 416

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
            D + L+DDIS HTLLHW+SSIESKSSHPMAAA ++H KLLS DIKP+ V+EFENFPGEG
Sbjct: 417 TDFQALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEG 476

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRS 240
           V GKIDG +IYIGS KIA RAGC +   L++EMKQGQT+GYVFC     GSF LSD+CRS
Sbjct: 477 VRGKIDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRS 536

Query: 241 GVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFKRD 300
           G KEG+EE+KS GIKTAMLTGDC AAAMHAQQ+L NA+DVIH ELLPKEKA ++KEFKRD
Sbjct: 537 GAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRD 596

Query: 301 -GPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKKC 360
            G TAMVGD  NDTPA ATADIGI+MG+S S LA++TGNVIL SND+ KIP+AI+LA+K 
Sbjct: 597 YGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKS 656

Query: 361 HAKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRETKHKHGK 420
           HAKVVQNVILSI TK AI+GLAI GHPL+WAAVL DVGTCLLVILNSMLLLR + HKHG 
Sbjct: 657 HAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGN 716

Query: 421 ---KSFKPYSTKHVTID 434
              KS K  STKH   D
Sbjct: 717 KCCKSSKLCSTKHGRCD 733

BLAST of Sed0014598 vs. ExPASy TrEMBL
Match: A0A6J1EPB0 (cadmium/zinc-transporting ATPase HMA2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436148 PE=3 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 5.6e-184
Identity = 337/437 (77.12%), Postives = 375/437 (85.81%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M E V+EAQNNKSKTQRFI+ECAKYYTP V+VISAC+AAIPAA RVHNLSHWFHLALVVL
Sbjct: 297 MAEFVEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVL 356

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VSA PCALILS P+AAF A+TKAAMAGVLIKG +HLEVLAKIKV+AFDKTGTITRGEFVV
Sbjct: 357 VSACPCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVV 416

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
            D + L+DDIS HTLLHW+SSIESKSSHPMAAA ++H KLLS DIKP+ V+EFENFPGEG
Sbjct: 417 TDFQALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEG 476

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRS 240
           V GKIDG +IYIGS KIA RAGC +   L++EMKQGQT+GYVFC     GSF LSD+CRS
Sbjct: 477 VRGKIDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRS 536

Query: 241 GVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFKRD 300
           G KEG+EE+KS GIKTAMLTGDC AAAMHAQQ+L NA+DVIH ELLPKEKA ++KEFKRD
Sbjct: 537 GAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRD 596

Query: 301 -GPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKKC 360
            G TAMVGD  NDTPA ATADIGI+MG+S S LA++TGNVIL SND+ KIP+AI+LA+K 
Sbjct: 597 YGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKS 656

Query: 361 HAKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRETKHKHGK 420
           HAKVVQNVILSI TK AI+GLAI GHPL+WAAVL DVGTCLLVILNSMLLLR + HKHG 
Sbjct: 657 HAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGN 716

Query: 421 ---KSFKPYSTKHVTID 434
              KS K  STKH   D
Sbjct: 717 KCCKSSKLCSTKHGRCD 733

BLAST of Sed0014598 vs. ExPASy TrEMBL
Match: A0A6J1K4T4 (putative inactive cadmium/zinc-transporting ATPase HMA3 OS=Cucurbita maxima OX=3661 GN=LOC111492308 PE=3 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 3.6e-183
Identity = 334/437 (76.43%), Postives = 376/437 (86.04%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M E V+EAQNNKSKTQRFI+ECAKYYTP V+VISAC+AAIPAA RVHNLSHWFHLALVVL
Sbjct: 297 MAEFVEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVL 356

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VSA PCALILS P+AAF A+TKAAMAGVLIKG +HLEVLAKIKV+AFDKTGTITRGEFVV
Sbjct: 357 VSACPCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVV 416

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
            + + L+DDI+ HTLLHW+SSIESKSSHPMAAA ++H KLLS+DIKP+ V+EFENFPGEG
Sbjct: 417 TNFQALRDDINFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEG 476

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRS 240
           V GKIDG +IYIGS KIA RAGC +   LD+EMKQGQT+GYVFCG    G+F LSD+CRS
Sbjct: 477 VRGKIDGKDIYIGSTKIAARAGCSSAMKLDEEMKQGQTLGYVFCGEMAVGAFGLSDSCRS 536

Query: 241 GVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFKRD 300
           G KE +E++KS GIKTAMLTGDC AAAMHAQQ+L NA+DVIH ELLPKEKA ++KEFKRD
Sbjct: 537 GAKEAMEQLKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANMIKEFKRD 596

Query: 301 -GPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKKC 360
            G TAMVGD  NDTPA ATADIGI+MG+S S LA++TGNVIL SND+ KIP+AI+LA+K 
Sbjct: 597 YGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKS 656

Query: 361 HAKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRETKHKHGK 420
           HAKVVQNVILSI TK AI+GLAI GHPL+WAAVL DVGTCLLVILNSMLLLR + HKHG 
Sbjct: 657 HAKVVQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGN 716

Query: 421 ---KSFKPYSTKHVTID 434
              KS K  STKH   D
Sbjct: 717 KCCKSSKLCSTKHGRCD 733

BLAST of Sed0014598 vs. ExPASy TrEMBL
Match: A0A5D3BT62 (Putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold29G001020 PE=3 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 4.7e-183
Identity = 338/439 (76.99%), Postives = 373/439 (84.97%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M ELV+EAQ NKSKTQ FI+ECAKYYTP V++ISACLAAIPAA+RVHNLSHW HLALVVL
Sbjct: 299 MAELVEEAQKNKSKTQTFIDECAKYYTPAVIIISACLAAIPAALRVHNLSHWLHLALVVL 358

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VSA PCALILS P+AAF A+TKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV
Sbjct: 359 VSACPCALILSTPVAAFCALTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 418

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
              + L+DDIS HTLL W+SSIESKSSHPMA A +++GKL S+D+KP+NV+EFENFPGEG
Sbjct: 419 THFQALRDDISFHTLLQWVSSIESKSSHPMATALVNYGKLHSIDLKPENVEEFENFPGEG 478

Query: 181 VHGKIDGNNIYIGSRKIALRAG-----CKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLS 240
           V GKIDGN+IYIGS+KIA RAG        + N D E +Q QT+GYVFCG T+ GSF L 
Sbjct: 479 VRGKIDGNDIYIGSKKIAARAGYDIPVSSELNNFDDETRQEQTLGYVFCGGTIIGSFGLL 538

Query: 241 DACRSGVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVK 300
           D+CRSGVKE IEEIKSFGIKTAMLTGDCRAAAMH Q++LGN  +VIH ELLPKEKA I+K
Sbjct: 539 DSCRSGVKEAIEEIKSFGIKTAMLTGDCRAAAMHVQEQLGNNFEVIHSELLPKEKANIIK 598

Query: 301 EFKR-DGPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIR 360
           EFK+ DG  AMVGD  NDTPA ATADIG++MG+S SALA+ETGNVIL SNDMRKIPK I+
Sbjct: 599 EFKKNDGAIAMVGDGLNDTPALATADIGMSMGISGSALATETGNVILMSNDMRKIPKVIK 658

Query: 361 LAKKCHAKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRETK 420
           LAK  H KVVQNVILSIGTKTAI+GLA  GHPLIWAAVL DVGTCLLVILNSMLLLR T 
Sbjct: 659 LAKTFHTKVVQNVILSIGTKTAILGLAFAGHPLIWAAVLADVGTCLLVILNSMLLLRGTD 718

Query: 421 HKHGK----KSFKPYSTKH 430
           HKHGK    KS KP  TKH
Sbjct: 719 HKHGKKKCCKSSKPCLTKH 737

BLAST of Sed0014598 vs. TAIR 10
Match: AT4G30110.1 (heavy metal atpase 2 )

HSP 1 Score: 560.1 bits (1442), Expect = 1.6e-159
Identity = 276/433 (63.74%), Postives = 359/433 (82.91%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M +LV+EAQN+K++TQRFI++C+KYYTP +++IS C  AIP A++VHNL HW HLALVVL
Sbjct: 284 MAKLVEEAQNSKTETQRFIDKCSKYYTPAIILISICFVAIPFALKVHNLKHWVHLALVVL 343

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VSA PC LILS P+A F A+TKAA +G+LIKG ++LE LAKIK++AFDKTGTITRGEF+V
Sbjct: 344 VSACPCGLILSTPVATFCALTKAATSGLLIKGADYLETLAKIKIVAFDKTGTITRGEFIV 403

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
            D ++L +DISL +LL+W+SS ESKSSHPMAAA + + + +SV+ KP+ V++++NFPGEG
Sbjct: 404 MDFQSLSEDISLQSLLYWVSSTESKSSHPMAAAVVDYARSVSVEPKPEAVEDYQNFPGEG 463

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRS 240
           ++GKIDG  +YIG+++IA RAGC +VP++D + K G+T+GYV+ G TLAG F+LSDACRS
Sbjct: 464 IYGKIDGKEVYIGNKRIASRAGCLSVPDIDVDTKGGKTIGYVYVGETLAGVFNLSDACRS 523

Query: 241 GVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFKR- 300
           GV + ++E+KS GIK AMLTGD  AAAMHAQ++LGNA+D++  ELLP++K+ I+K+ KR 
Sbjct: 524 GVAQAMKELKSLGIKIAMLTGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKRE 583

Query: 301 DGPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKKC 360
           +GPTAMVGD  ND PA ATADIGI+MGVS SALA+ETGN+IL SND+R+IP+AI+LAK+ 
Sbjct: 584 EGPTAMVGDGLNDAPALATADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRA 643

Query: 361 HAKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRETKHKHGK 420
             KVV+NV++SI  K AI+ LA  GHPLIWAAVL DVGTCLLVILNSMLLL + KHK G 
Sbjct: 644 KRKVVENVVISITMKGAILALAFAGHPLIWAAVLADVGTCLLVILNSMLLLSD-KHKTGN 703

Query: 421 KSFKPYSTKHVTI 433
           K ++  S+  V I
Sbjct: 704 KCYRESSSSSVLI 715

BLAST of Sed0014598 vs. TAIR 10
Match: AT2G19110.1 (heavy metal atpase 4 )

HSP 1 Score: 554.3 bits (1427), Expect = 8.9e-158
Identity = 265/428 (61.92%), Postives = 355/428 (82.94%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M +LV+EAQ++K+K+QR I++C++YYTP ++++SAC+A +P  M+VHNL HWFHLALVVL
Sbjct: 294 MAKLVEEAQSSKTKSQRLIDKCSQYYTPAIILVSACVAIVPVIMKVHNLKHWFHLALVVL 353

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VS  PC LILS P+A F A+TKAA +G+LIK  ++L+ L+KIK++AFDKTGTITRGEF+V
Sbjct: 354 VSGCPCGLILSTPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKTGTITRGEFIV 413

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
            D ++L  DI+L +LL+W+SS+ESKSSHPMAA  + + K +SV+ +P+ V++++NFPGEG
Sbjct: 414 IDFKSLSRDINLRSLLYWVSSVESKSSHPMAATIVDYAKSVSVEPRPEEVEDYQNFPGEG 473

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRS 240
           ++GKIDGN+I+IG++KIA RAGC TVP ++ + K G+TVGYV+ G  LAG F+LSDACRS
Sbjct: 474 IYGKIDGNDIFIGNKKIASRAGCSTVPEIEVDTKGGKTVGYVYVGERLAGFFNLSDACRS 533

Query: 241 GVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALDVIHFELLPKEKARIVKEFKRD 300
           GV + + E+KS GIKTAMLTGD +AAAMHAQ++LGN LDV+H +LLP++K+RI++EFK++
Sbjct: 534 GVSQAMAELKSLGIKTAMLTGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKE 593

Query: 301 GPTAMVGDSSNDTPAFATADIGITMGVSSSALASETGNVILTSNDMRKIPKAIRLAKKCH 360
           GPTAMVGD  ND PA ATADIGI+MG+S SALA++TGN+IL SND+R+IP+A++LA++  
Sbjct: 594 GPTAMVGDGVNDAPALATADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRAR 653

Query: 361 AKVVQNVILSIGTKTAIIGLAIGGHPLIWAAVLTDVGTCLLVILNSMLLLRETKHKHGKK 420
            KVV+NV LSI  K  I+ LA  GHPLIWAAVL DVGTCLLVI NSMLLLRE K    KK
Sbjct: 654 RKVVENVCLSIILKAGILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLREKKKIGNKK 713

Query: 421 SFKPYSTK 429
            ++  ++K
Sbjct: 714 CYRASTSK 721

BLAST of Sed0014598 vs. TAIR 10
Match: AT4G30120.1 (heavy metal atpase 3 )

HSP 1 Score: 311.6 bits (797), Expect = 1.0e-84
Identity = 151/253 (59.68%), Postives = 202/253 (79.84%), Query Frame = 0

Query: 1   MGELVKEAQNNKSKTQRFIEECAKYYTPVVLVISACLAAIPAAMRVHNLSHWFHLALVVL 60
           M +LV+EAQ +++KTQRFI++C++YYTP V+V +AC A IP  ++V +LSHWFHLALVVL
Sbjct: 290 MTKLVEEAQKSQTKTQRFIDKCSRYYTPAVVVSAACFAVIPVLLKVQDLSHWFHLALVVL 349

Query: 61  VSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVV 120
           VS  PC LILS P+A F A+TKAA +G LIK G+ LE LAKIK++AFDKTGTIT+ EF+V
Sbjct: 350 VSGCPCGLILSTPVATFCALTKAATSGFLIKTGDCLETLAKIKIVAFDKTGTITKAEFMV 409

Query: 121 RDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIKPKNVDEFENFPGEG 180
            D R+L   I+LH LL+W+SSIE KSSHPMAAA I + + +SV+ KP  V+ F+NFPGEG
Sbjct: 410 SDFRSLSPSINLHKLLYWVSSIECKSSHPMAAALIDYARSVSVEPKPDIVENFQNFPGEG 469

Query: 181 VHGKIDGNNIYIGSRKIALRAGCKT--VPNLDKEMKQGQTVGYVFCGATLAGSFSLSDAC 240
           V+G+IDG +IYIG+++IA RAGC T  VP+++  MK+G+T+GY++ GA L GSF+L D C
Sbjct: 470 VYGRIDGQDIYIGNKRIAQRAGCLTDNVPDIEATMKRGKTIGYIYMGAKLTGSFNLLDGC 529

Query: 241 RSGVKEGIEEIKS 252
           R GV + ++E+KS
Sbjct: 530 RYGVAQALKELKS 542

BLAST of Sed0014598 vs. TAIR 10
Match: AT1G63440.1 (heavy metal atpase 5 )

HSP 1 Score: 155.6 bits (392), Expect = 9.2e-38
Identity = 121/405 (29.88%), Postives = 204/405 (50.37%), Query Frame = 0

Query: 4   LVKEAQNNKSKTQRFIEECAKYYTPVVLVISACL---------------AAIPAAMRVHN 63
           LV+ AQ  K+  Q+  +  +K++ P+V+ +S                  + IP++M    
Sbjct: 544 LVESAQLAKAPVQKLADRISKFFVPLVIFLSFSTWLAWFLAGKLHWYPESWIPSSMDSFE 603

Query: 64  LSHWFHLALVVLVSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFD 123
           L+  F ++  V+V A PCAL L+ P A        A  GVLIKGG  LE   K+  + FD
Sbjct: 604 LALQFGIS--VMVIACPCALGLATPTAVMVGTGVGASQGVLIKGGQALERAHKVNCIVFD 663

Query: 124 KTGTITRGEFVVRDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISHGKLLSVDIK-- 183
           KTGT+T G+ VV   + L++ + L      +++ E  S HP+A A + + K    D +  
Sbjct: 664 KTGTLTMGKPVVVKTKLLKNMV-LREFYELVAATEVNSEHPLAKAIVEYAKKFRDDEENP 723

Query: 184 --PKNVDEFENFPGEGVHGKIDGNNIYIGSRKIALRAGCKTVPNLDKEM-----KQGQTV 243
             P+  D F +  G+GV   + G  I +G++ + +      +P+  +E+        QT 
Sbjct: 724 AWPEACD-FVSITGKGVKATVKGREIMVGNKNL-MNDHKVIIPDDAEELLADSEDMAQTG 783

Query: 244 GYVFCGATLAGSFSLSDACRSGVKEGIEEIKSFGIKTAMLTGDCRAAAMHAQQKLGNALD 303
             V   + L G  S+SD  +   +E I  +KS  IK+ M+TGD    A    +++G  +D
Sbjct: 784 ILVSINSELIGVLSVSDPLKPSAREAISILKSMNIKSIMVTGDNWGTANSIAREVG--ID 843

Query: 304 VIHFELLPKEKARIVKEFKRDG-PTAMVGDSSNDTPAFATADIGITMGVSSSALASETGN 363
            +  E  P++KA  VKE +  G   AMVGD  ND+PA   AD+G+ +G  +  +A E  +
Sbjct: 844 SVIAEAKPEQKAEKVKELQAAGHVVAMVGDGINDSPALVAADVGMAIGAGTD-IAIEAAD 903

Query: 364 VILTSNDMRKIPKAIRLAKKCHAKVVQNVILSIGTKTAIIGLAIG 384
           ++L  +++  +  AI L++K  +++  N + ++G     I +A G
Sbjct: 904 IVLMKSNLEDVITAIDLSRKTFSRIRLNYVWALGYNLMGIPIAAG 940

BLAST of Sed0014598 vs. TAIR 10
Match: AT5G44790.1 (copper-exporting ATPase / responsive-to-antagonist 1 / copper-transporting ATPase (RAN1) )

HSP 1 Score: 155.6 bits (392), Expect = 9.2e-38
Identity = 127/456 (27.85%), Postives = 220/456 (48.25%), Query Frame = 0

Query: 4   LVKEAQNNKSKTQRFIEECAKYYTPVVLV----------ISACLAAIPAAMRVHNLSHW- 63
           LV+ AQ +K+  Q+F +  A  + PVV+           I   + A P      N +H+ 
Sbjct: 541 LVETAQMSKAPIQKFADYVASIFVPVVITLALFTLVGWSIGGAVGAYPDEWLPENGTHFV 600

Query: 64  --FHLALVVLVSAFPCALILSAPIAAFFAVTKAAMAGVLIKGGNHLEVLAKIKVMAFDKT 123
                ++ V+V A PCAL L+ P A   A    A  GVLIKGG+ LE   K+K + FDKT
Sbjct: 601 FSLMFSISVVVIACPCALGLATPTAVMVATGVGATNGVLIKGGDALEKAHKVKYVIFDKT 660

Query: 124 GTITRGEFVVRDIRTLQDDISLHTLLHWISSIESKSSHPMAAAFISH------------- 183
           GT+T+G+  V   +    ++     L  ++S E+ S HP+A A +++             
Sbjct: 661 GTLTQGKATVTTTKVF-SEMDRGEFLTLVASAEASSEHPLAKAIVAYARHFHFFDESTED 720

Query: 184 GKLLSVDIKPK----NVDEFENFPGEGVHGKIDGNNIYIGSRKIALRAGC-------KTV 243
           G+  + D++      +  +F   PG+G+   ++   I +G+RK+             K V
Sbjct: 721 GETNNKDLQNSGWLLDTSDFSALPGKGIQCLVNEKMILVGNRKLMSENAINIPDHVEKFV 780

Query: 244 PNLDKEMKQGQTVGYVFCGATLAGSFSLSDACRSGVKEGIEEIKSFGIKTAMLTGDCRAA 303
            +L++  K G  V Y      L G   ++D  +      +E +   G++  M+TGD    
Sbjct: 781 EDLEESGKTGVIVAY---NGKLVGVMGIADPLKREAALVVEGLLRMGVRPIMVTGDNWRT 840

Query: 304 AMHAQQKLGNALDVIHFELLPKEKARIVKEFKRDGPT-AMVGDSSNDTPAFATADIGITM 363
           A    +++G  ++ +  E++P  KA +++  ++DG T AMVGD  ND+PA A AD+G+ +
Sbjct: 841 ARAVAKEVG--IEDVRAEVMPAGKADVIRSLQKDGSTVAMVGDGINDSPALAAADVGMAI 900

Query: 364 GVSSSALASETGNVILTSNDMRKIPKAIRLAKKCHAKVVQNVILSIGTKTAIIGLAIG-G 415
           G  +  +A E  + +L  N++  +  AI L++K   ++  N + ++      I +A G  
Sbjct: 901 GAGTD-VAIEAADYVLMRNNLEDVITAIDLSRKTLTRIRLNYVFAMAYNVVSIPIAAGVF 960

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153334.16.3e-19080.37putative inactive cadmium/zinc-transporting ATPase HMA3 [Momordica charantia][more]
XP_038891185.11.0e-18779.21LOW QUALITY PROTEIN: putative inactive cadmium/zinc-transporting ATPase HMA3 [Be... [more]
XP_023545589.16.1e-18577.12putative inactive cadmium/zinc-transporting ATPase HMA3 [Cucurbita pepo subsp. p... [more]
KAG6598888.16.8e-18477.12Cadmium/zinc-transporting ATPase HMA2, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022929619.11.2e-18377.12cadmium/zinc-transporting ATPase HMA2-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SZW42.3e-15863.74Cadmium/zinc-transporting ATPase HMA2 OS=Arabidopsis thaliana OX=3702 GN=HMA2 PE... [more]
O644741.3e-15661.92Putative cadmium/zinc-transporting ATPase HMA4 OS=Arabidopsis thaliana OX=3702 G... [more]
P0CW784.2e-14461.30Cadmium/zinc-transporting ATPase HMA3 OS=Arabidopsis thaliana OX=3702 GN=HMA3 PE... [more]
A3BF395.5e-13659.14Cadmium/zinc-transporting ATPase HMA2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q8H3841.1e-11251.45Cadmium/zinc-transporting ATPase HMA3 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Match NameE-valueIdentityDescription
A0A6J1DIS03.1e-19080.37putative inactive cadmium/zinc-transporting ATPase HMA3 OS=Momordica charantia O... [more]
A0A6J1EUV85.6e-18477.12cadmium/zinc-transporting ATPase HMA2-like isoform X2 OS=Cucurbita moschata OX=3... [more]
A0A6J1EPB05.6e-18477.12cadmium/zinc-transporting ATPase HMA2-like isoform X1 OS=Cucurbita moschata OX=3... [more]
A0A6J1K4T43.6e-18376.43putative inactive cadmium/zinc-transporting ATPase HMA3 OS=Cucurbita maxima OX=3... [more]
A0A5D3BT624.7e-18376.99Putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X1 OS=Cucumis me... [more]
Match NameE-valueIdentityDescription
AT4G30110.11.6e-15963.74heavy metal atpase 2 [more]
AT2G19110.18.9e-15861.92heavy metal atpase 4 [more]
AT4G30120.11.0e-8459.68heavy metal atpase 3 [more]
AT1G63440.19.2e-3829.88heavy metal atpase 5 [more]
AT5G44790.19.2e-3827.85copper-exporting ATPase / responsive-to-antagonist 1 / copper-transporting ATPas... [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00119CATATPASEcoord: 106..120
score: 48.57
coord: 252..262
score: 54.0
coord: 306..325
score: 53.59
coord: 330..342
score: 25.77
NoneNo IPR availablePFAMPF00702Hydrolasecoord: 102..320
e-value: 4.3E-28
score: 99.0
NoneNo IPR availablePFAMPF00122E1-E2_ATPasecoord: 3..84
e-value: 4.6E-13
score: 49.0
NoneNo IPR availableSFLDSFLDS00003Haloacid_Dehalogenasecoord: 88..358
e-value: 3.5E-40
score: 132.8
NoneNo IPR availablePANTHERPTHR48085CADMIUM/ZINC-TRANSPORTING ATPASE HMA2-RELATEDcoord: 1..428
NoneNo IPR availablePANTHERPTHR48085:SF4CADMIUM/ZINC-TRANSPORTING ATPASE HMA4-RELATEDcoord: 1..428
IPR027256P-type ATPase, subfamily IBTIGRFAMTIGR01525TIGR01525coord: 3..409
e-value: 2.4E-117
score: 391.1
IPR023299P-type ATPase, cytoplasmic domain NGENE3D3.40.1110.10coord: 117..238
e-value: 2.2E-67
score: 229.5
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 98..357
e-value: 2.2E-67
score: 229.5
IPR001757P-type ATPaseTIGRFAMTIGR01494TIGR01494coord: 4..385
e-value: 3.3E-44
score: 149.1
IPR044492P-type ATPase, haloacid dehalogenase domainSFLDSFLDF00027p-type_atpasecoord: 88..358
e-value: 3.5E-40
score: 132.8
IPR018303P-type ATPase, phosphorylation sitePROSITEPS00154ATPASE_E1_E2coord: 108..114
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 105..407
IPR023298P-type ATPase, transmembrane domain superfamilySUPERFAMILY81665Calcium ATPase, transmembrane domain Mcoord: 8..198

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0014598.1Sed0014598.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098655 cation transmembrane transport
biological_process GO:0071585 detoxification of cadmium ion
biological_process GO:0006812 cation transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005774 vacuolar membrane
molecular_function GO:0019829 ATPase-coupled cation transmembrane transporter activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0140603 obsolete ATP hydrolysis activity
molecular_function GO:0005215 transporter activity