Sed0014532 (gene) Chayote v1

Overview
NameSed0014532
Typegene
OrganismSechium edule (Chayote v1)
DescriptionPoly(A)-specific ribonuclease
LocationLG01: 70525770 .. 70527042 (+)
RNA-Seq ExpressionSed0014532
SyntenySed0014532
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAACACGTCGGCCAAAATTTCAAGTTCTTTCAATAGGGAGGTTTGGTCTCACAATCTTCGCGAAGAGATAGAACTTATTAAAGAGTATTTGCCAGAGTTTCCAATAGTAGCCATTGATACTGAATTTCCAGGTATATATATATATATAAATATAAGATTATTTTAAATTAAAAAATATTCTTGAATTAGTATAGAAAGGGTTGGTGGCTTCGTTGTTTGGTGGTTATGATTTCATTGTTCGATGATAATTGGGTTGGCAGCTTCGTTGTTTGGTGGCTATGGTTTCTTTGTTTGATGGTAATTCAGTTCAACGCTACTGTAATGAGTTTGGATTCTGAAAATCGGTATTTGGATCTCAAGCACTTAGAGAACAGTAGTACCCTCCAAGTAGGGTTTGTGGTCCCCCTCCCCTAATTTATCTTGAAGTTTGATTGTGAGATTCATATTTTCGGATTGGATACCCAGGCTTTCTTCGGTATACTCCAAAAGGTGTTTCGGAAGAAGAGTTTTACGAGTGTCTTCGATTCAACGTCAACCGGTTGAGCATACTTCAGCTCGGTCTTACACTCACGGACGACAAAGGAAGAATCGGGCCGACATGGGAGTTCAACTTTAGAGACTTTGATTTGATTTCTGATATGCATGGTGTTTCGTCGTTGGATTCGTTTCTCAAGAACAAGGGTTTTGACTTTGAAAAGTTAAGAAAAGATGGAATTGCACCCGAGGAATTCTCAGCAATGTTTCTGCCGATATGTCGTAGTGGTCGAGTTGACAAATGGGTCACATTTCACGGATTTTACGATATTGCTTACCTAATGAAGCTTTTGAAGATCAGATTCATAGCGACATCGATGGCGGCGTTCGCAACCACTGCTCAACGATTGTTGGGAACGATCGTCGATCTCAAGCACATGGCTCGGTATTGTCACGGCTTGCTTAATGGCGACCTAGGGCTAAAAAAACTAGCCAGATTGGTCAACGTTAAGCGAGTTGGTATTGCACACTTTGCTGGTTCTGATAGTTTGCTCACTGCTTCTGTTTATGCCAAGATGAAATTGATGTTTAAGCTCAACCCTGAGCAATGTGAAGGGTTTTTGTATGGACTGCCGCACTCGATTCGATCTACCCGACATACTGTCAAGGTTATCGATCCATCCACAGCTACTTCGTCGCCAATGTTGAAATACCCGTCAATGCCACAATGCTCGTCGGCATCAGCTTACAGATCATCGCCTATATTCGTGGGCGACTTTTTAATTTTTGGCTGA

mRNA sequence

ATGAAGAACACGTCGGCCAAAATTTCAAGTTCTTTCAATAGGGAGGTTTGGTCTCACAATCTTCGCGAAGAGATAGAACTTATTAAAGAGTATTTGCCAGAGTTTCCAATAGTAGCCATTGATACTGAATTTCCAGGCTTTCTTCGGTATACTCCAAAAGGTGTTTCGGAAGAAGAGTTTTACGAGTGTCTTCGATTCAACGTCAACCGGTTGAGCATACTTCAGCTCGGTCTTACACTCACGGACGACAAAGGAAGAATCGGGCCGACATGGGAGTTCAACTTTAGAGACTTTGATTTGATTTCTGATATGCATGGTGTTTCGTCGTTGGATTCGTTTCTCAAGAACAAGGGTTTTGACTTTGAAAAGTTAAGAAAAGATGGAATTGCACCCGAGGAATTCTCAGCAATGTTTCTGCCGATATGTCGTAGTGGTCGAGTTGACAAATGGGTCACATTTCACGGATTTTACGATATTGCTTACCTAATGAAGCTTTTGAAGATCAGATTCATAGCGACATCGATGGCGGCGTTCGCAACCACTGCTCAACGATTGTTGGGAACGATCGTCGATCTCAAGCACATGGCTCGGTATTGTCACGGCTTGCTTAATGGCGACCTAGGGCTAAAAAAACTAGCCAGATTGGTCAACGTTAAGCGAGTTGGTATTGCACACTTTGCTGGTTCTGATAGTTTGCTCACTGCTTCTGTTTATGCCAAGATGAAATTGATGTTTAAGCTCAACCCTGAGCAATGTGAAGGGTTTTTGTATGGACTGCCGCACTCGATTCGATCTACCCGACATACTGTCAAGGTTATCGATCCATCCACAGCTACTTCGTCGCCAATGTTGAAATACCCGTCAATGCCACAATGCTCGTCGGCATCAGCTTACAGATCATCGCCTATATTCGTGGGCGACTTTTTAATTTTTGGCTGA

Coding sequence (CDS)

ATGAAGAACACGTCGGCCAAAATTTCAAGTTCTTTCAATAGGGAGGTTTGGTCTCACAATCTTCGCGAAGAGATAGAACTTATTAAAGAGTATTTGCCAGAGTTTCCAATAGTAGCCATTGATACTGAATTTCCAGGCTTTCTTCGGTATACTCCAAAAGGTGTTTCGGAAGAAGAGTTTTACGAGTGTCTTCGATTCAACGTCAACCGGTTGAGCATACTTCAGCTCGGTCTTACACTCACGGACGACAAAGGAAGAATCGGGCCGACATGGGAGTTCAACTTTAGAGACTTTGATTTGATTTCTGATATGCATGGTGTTTCGTCGTTGGATTCGTTTCTCAAGAACAAGGGTTTTGACTTTGAAAAGTTAAGAAAAGATGGAATTGCACCCGAGGAATTCTCAGCAATGTTTCTGCCGATATGTCGTAGTGGTCGAGTTGACAAATGGGTCACATTTCACGGATTTTACGATATTGCTTACCTAATGAAGCTTTTGAAGATCAGATTCATAGCGACATCGATGGCGGCGTTCGCAACCACTGCTCAACGATTGTTGGGAACGATCGTCGATCTCAAGCACATGGCTCGGTATTGTCACGGCTTGCTTAATGGCGACCTAGGGCTAAAAAAACTAGCCAGATTGGTCAACGTTAAGCGAGTTGGTATTGCACACTTTGCTGGTTCTGATAGTTTGCTCACTGCTTCTGTTTATGCCAAGATGAAATTGATGTTTAAGCTCAACCCTGAGCAATGTGAAGGGTTTTTGTATGGACTGCCGCACTCGATTCGATCTACCCGACATACTGTCAAGGTTATCGATCCATCCACAGCTACTTCGTCGCCAATGTTGAAATACCCGTCAATGCCACAATGCTCGTCGGCATCAGCTTACAGATCATCGCCTATATTCGTGGGCGACTTTTTAATTTTTGGCTGA

Protein sequence

MKNTSAKISSSFNREVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYTPKGVSEEEFYECLRFNVNRLSILQLGLTLTDDKGRIGPTWEFNFRDFDLISDMHGVSSLDSFLKNKGFDFEKLRKDGIAPEEFSAMFLPICRSGRVDKWVTFHGFYDIAYLMKLLKIRFIATSMAAFATTAQRLLGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLLTASVYAKMKLMFKLNPEQCEGFLYGLPHSIRSTRHTVKVIDPSTATSSPMLKYPSMPQCSSASAYRSSPIFVGDFLIFG
Homology
BLAST of Sed0014532 vs. NCBI nr
Match: XP_022154798.1 (probable CCR4-associated factor 1 homolog 6 [Momordica charantia])

HSP 1 Score: 417.2 bits (1071), Expect = 1.3e-112
Identity = 205/310 (66.13%), Postives = 241/310 (77.74%), Query Frame = 0

Query: 2   KNTSAKISSSFNREVWSHNLREEIELIKEYLPEFPIVAIDTEF----------------- 61
           K+ +A  S   NREVWS+NL +E+E++ EYLPEFPIVAIDTEF                 
Sbjct: 7   KSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRIYFLSLA 66

Query: 62  ------------PGFLRYTPKGVSEEEFYECLRFNVNRLSILQLGLTLTDDKGRIGPTWE 121
                       PGFLRYTPKGVSEEEFY+CLRFNVNRL+ILQLGLTL +  G +  TWE
Sbjct: 67  GKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNVAMTWE 126

Query: 122 FNFRDFDLISDMHGVSSLDSFLKNKGFDFEKLRKDGIAPEEFSAMFLPICRSGRVDKWVT 181
           FNFRDFD ISDM GVSSLDSFLKNKGFDF KL++DGIA E+F+ +FLPICRSGR+++W+T
Sbjct: 127 FNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGRIERWIT 186

Query: 182 FHGFYDIAYLMKLLKIRFIATSMAAFATTAQRLLGTIVDLKHMARYCHGLLNGDLGLKKL 241
           FHGFYDIAYL+KLLKI++I  SMA FA TAQ LLGT+ DLKHM RYC GLL+GDLGLKKL
Sbjct: 187 FHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGDLGLKKL 246

Query: 242 ARLVNVKRVGIAHFAGSDSLLTASVYAKMKLMFKLNPEQCEGFLYGLPHSIRSTRHTVKV 283
           A+L++VKR+GIAHFAGSDSLLTA+VY KMK MFK+  EQ EGFLYGLPH I++ +  VK+
Sbjct: 247 AKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQMFKVPVEQREGFLYGLPHRIQAFQPIVKI 306

BLAST of Sed0014532 vs. NCBI nr
Match: XP_022146384.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 415.2 bits (1066), Expect = 4.9e-112
Identity = 207/307 (67.43%), Postives = 241/307 (78.50%), Query Frame = 0

Query: 2   KNTSAKISSSFNREVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYTPKGVSEEEFY 61
           K+T+A  S   NREVWS NLREE+EL+ EYLPEFP+V ID EFPGFL  TPKG+SEEEFY
Sbjct: 26  KSTAAPRSICHNREVWSLNLREELELLNEYLPEFPVVVIDMEFPGFLWCTPKGISEEEFY 85

Query: 62  ECLRFNVNRLSILQLGLTLTDDKGRIGPTWEFNFRDFDLISDMHGVSSLDSFLKNKGFDF 121
            CLRFNVNRL+ILQLGLTL D KG I  TWEFNFRDFD +SDM  VSSLDSFLKNKGFDF
Sbjct: 86  HCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDF 145

Query: 122 EKLRKDGIAPEEFSAMFLPICRSGRVDKWVTFHGFYDIAYLMKLLKIRFIATSMAAFATT 181
            KL+K GIA E+F+ +FLPICRSG++ +W+TFHGFYDI YL+KLLKI+ I  SMA FATT
Sbjct: 146 YKLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGFYDITYLLKLLKIKSIPISMATFATT 205

Query: 182 AQRLLGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLLTASVYAKM 241
           AQ LLGT+ DLKHMARYC GLL+G+LGLKKLA+L++VKR+  AHFA SD+L T SVY KM
Sbjct: 206 AQHLLGTVSDLKHMARYCDGLLDGNLGLKKLAKLLDVKRIDNAHFACSDNLFTTSVYTKM 265

Query: 242 KLMFKLNPEQCEGFLYGLPHSIRSTRHTVKVIDPSTATSSPML--KYPSMPQCSSASAYR 301
           K +FKL  EQCEGFLYGLP+ I   +   K+IDPST T  P L  K+  +  CS      
Sbjct: 266 KQIFKLPLEQCEGFLYGLPYPINGFQSVAKIIDPSTMTPPPPLPSKFSILSFCS------ 325

Query: 302 SSPIFVG 307
           S+P+ VG
Sbjct: 326 STPLLVG 326

BLAST of Sed0014532 vs. NCBI nr
Match: XP_022928830.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 394.0 bits (1011), Expect = 1.2e-105
Identity = 191/275 (69.45%), Postives = 222/275 (80.73%), Query Frame = 0

Query: 5   SAKISSSFNREVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYTPKGVSEEEFYECL 64
           +A +   + REVWSHNL EEIEL+K YL EFPIVAIDTEFPGFLR+TPKGVS+EEFY+CL
Sbjct: 8   AAIVGGQWKREVWSHNLLEEIELMKHYLSEFPIVAIDTEFPGFLRWTPKGVSDEEFYQCL 67

Query: 65  RFNVNRLSILQLGLTLTDDKGRIGPTWEFNFRDFDLISDMHGVSSLDSFLKNKGFDFEKL 124
           +FNVNRLSILQLGLTLTDD G+IG TWEFNF+DFD IS+M GV SLDSFLKNKGF+F KL
Sbjct: 68  QFNVNRLSILQLGLTLTDDNGKIGMTWEFNFQDFDQISNMEGVYSLDSFLKNKGFNFNKL 127

Query: 125 RKDGIAPEEFSAMFLPICRSGRVDKWVTFHGFYDIAYLMKLLKIRFIATSMAAFATTAQR 184
           R  GIAP+EFS  F P+ RSGR+ KW+TFHG YDIAYLMK   I  + TSM+ FA  A  
Sbjct: 128 RSCGIAPQEFSRQFKPVIRSGRIQKWITFHGLYDIAYLMKFNNIISMPTSMSMFAMIAAH 187

Query: 185 LLGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLLTASVYAKMKLM 244
           LLGT+ DLKHMARY   L NG+LGLKKLA+L+ + RVG AHFAGSDSLLTASVYAKMK++
Sbjct: 188 LLGTVTDLKHMARYSDYLFNGNLGLKKLAKLLQINRVGTAHFAGSDSLLTASVYAKMKVI 247

Query: 245 FKLNPEQCEGFLYGLPHSIRSTRHTVKVIDPSTAT 280
            K+ P+  EGFLYGLP+ I+  R  V ++DPS  T
Sbjct: 248 MKVEPKIHEGFLYGLPYPIQGYRPPVVIVDPSKLT 282

BLAST of Sed0014532 vs. NCBI nr
Match: XP_023511526.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 389.0 bits (998), Expect = 3.7e-104
Identity = 185/274 (67.52%), Postives = 223/274 (81.39%), Query Frame = 0

Query: 6   AKISSSFNREVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYTPKGVSEEEFYECLR 65
           A +   + R+VWSHNL EEIELIK+ L EFPIVAIDTEFPGFLR+TPKGVS+EEFYECL+
Sbjct: 9   AIVEGQWKRDVWSHNLSEEIELIKQCLSEFPIVAIDTEFPGFLRWTPKGVSDEEFYECLQ 68

Query: 66  FNVNRLSILQLGLTLTDDKGRIGPTWEFNFRDFDLISDMHGVSSLDSFLKNKGFDFEKLR 125
           FNVNRLSILQLGLTLT+D G+IG TWEFNF+DFD IS+M GV SLDSFLKNKGF+F  LR
Sbjct: 69  FNVNRLSILQLGLTLTNDNGKIGMTWEFNFQDFDKISNMEGVYSLDSFLKNKGFNFNILR 128

Query: 126 KDGIAPEEFSAMFLPICRSGRVDKWVTFHGFYDIAYLMKLLKIRFIATSMAAFATTAQRL 185
             GIAP+EF   F PI RSG++ +W+TFHG YDIAYLMK   IR + TSM+ FA +A  +
Sbjct: 129 SYGIAPQEFGKQFNPILRSGKIQRWITFHGLYDIAYLMKFTYIRCMPTSMSMFAISAAHI 188

Query: 186 LGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLLTASVYAKMKLMF 245
           LGT+ DLKHMARY + LLNG+LGLK+LA+L+ V R G AHFAGSDSLLTA+VYAKMK++ 
Sbjct: 189 LGTVTDLKHMARYSNNLLNGNLGLKRLAKLLQVNRTGTAHFAGSDSLLTAAVYAKMKIIM 248

Query: 246 KLNPEQCEGFLYGLPHSIRSTRHTVKVIDPSTAT 280
           K+ P++ EGFLYGLP+ I+  R  + ++DPS  T
Sbjct: 249 KVEPKKHEGFLYGLPYPIQGYRRPIVIVDPSKLT 282

BLAST of Sed0014532 vs. NCBI nr
Match: XP_022963784.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 386.7 bits (992), Expect = 1.9e-103
Identity = 190/274 (69.34%), Postives = 219/274 (79.93%), Query Frame = 0

Query: 6   AKISSSFNREVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYTPKGVSEEEFYECLR 65
           A +   + R+VWSHNL EEIELIK+ L EFPIVAIDTEFPGFLR+TPKGVS+EEFYECL+
Sbjct: 9   AIVEGQWKRDVWSHNLLEEIELIKQCLSEFPIVAIDTEFPGFLRWTPKGVSDEEFYECLQ 68

Query: 66  FNVNRLSILQLGLTLTDDKGRIGPTWEFNFRDFDLISDMHGVSSLDSFLKNKGFDFEKLR 125
           FNVNRLSILQLGLTLT+D G+IG TWEFNFRDFD IS+M GV SLDSFLKNKGF+F  LR
Sbjct: 69  FNVNRLSILQLGLTLTNDNGKIGMTWEFNFRDFDKISNMEGVYSLDSFLKNKGFNFNTLR 128

Query: 126 KDGIAPEEFSAMFLPICRSGRVDKWVTFHGFYDIAYLMKLLKIRFIATSMAAFATTAQRL 185
             GIAPEEF   F PI RSGR+ +W+TFHG YDIAYLMK   IR + TSM+ FA TA  +
Sbjct: 129 SYGIAPEEFGTQFSPILRSGRIHRWITFHGLYDIAYLMKFTNIRCMPTSMSIFAITAAHI 188

Query: 186 LGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLLTASVYAKMKLMF 245
           LGT+ DLKHMA Y   LL+G+LGLKKLA+L+ V R G AHFAGSDSLLTASVYAKMK++ 
Sbjct: 189 LGTVTDLKHMAWY--SLLDGNLGLKKLAKLLQVNRTGTAHFAGSDSLLTASVYAKMKIIM 248

Query: 246 KLNPEQCEGFLYGLPHSIRSTRHTVKVIDPSTAT 280
           K+ P   EGFLYGLP+ I+  R  V ++DPS  T
Sbjct: 249 KVEPNIHEGFLYGLPYPIQGYRRPVVIVDPSKLT 280

BLAST of Sed0014532 vs. ExPASy Swiss-Prot
Match: Q9SAI2 (Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=CAF1-6 PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.1e-45
Identity = 109/262 (41.60%), Postives = 155/262 (59.16%), Query Frame = 0

Query: 14  REVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYTPKGV---SEEEFYECLRFNVNR 73
           REVW+ NL+EE++LI++ + +FP VA+DTEFPG +   P G    + +  YE L+ NVN 
Sbjct: 13  REVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIV-VRPVGTFKSNADYHYETLKTNVNI 72

Query: 74  LSILQLGLTLTDDKGRIGPT--------WEFNFRDFDLISDMHGVSSLDSFLKNKGFDFE 133
           L ++QLGLT ++++G + PT        W+FNFR+FDL SD+  + S++  LK  G D  
Sbjct: 73  LKMIQLGLTFSNEQGNL-PTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLA 132

Query: 134 KLRKDGIAPEEFSAMFLPICRSGRV----DKWVTFHGFYDIAYLMKLLKIRFIATSMAAF 193
           K   DGI  + F+ + +    SG V      WVTFH  YD  YL+KLL  + +  S   F
Sbjct: 133 KNTLDGIDSKRFAELLM---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDF 192

Query: 194 ATTAQRLLGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLLTASVY 253
                    T+ D+KH+ ++C+ L     GL KLA L+ V+RVGI H AGSDSLLT+  +
Sbjct: 193 FKLINVYFPTVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTF 252

Query: 254 AKMKLMFKLNP-EQCEGFLYGL 260
            K+K  F + P  +  G LYGL
Sbjct: 253 RKLKENFFVGPLHKYSGVLYGL 265

BLAST of Sed0014532 vs. ExPASy Swiss-Prot
Match: Q9S9P2 (Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=CAF1-2 PE=1 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 5.3e-45
Identity = 108/267 (40.45%), Postives = 159/267 (59.55%), Query Frame = 0

Query: 14  REVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYT--------PKGVSEEEFYECLR 73
           REVW+HNL +E+ LI++ + +FP VA+DTEFPG +  T        P  +  E  Y+ L+
Sbjct: 17  REVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGIVCKTVTANPNPNPYSIHYEYNYDTLK 76

Query: 74  FNVNRLSILQLGLTLTDDKGRIGPT--------WEFNFRDFDLISDMHGVSSLDSFLKNK 133
            NVN L ++QLGLTL+D+KG + PT        W+FNFR+F++ISDM  + S++  L+  
Sbjct: 77  ANVNMLKLIQLGLTLSDEKGNL-PTCGTNKQCIWQFNFREFNVISDMFALDSIE-LLRKS 136

Query: 134 GFDFEKLRKDGIAPEEFSAMFLPICRSGRV--DK--WVTFHGFYDIAYLMKLLKIRFIAT 193
             D EK  + G+  + F+ + +    SG V  DK  WVTFH  YD  YL+KLL  + +  
Sbjct: 137 AIDLEKNNECGVDAKRFAELLM---GSGVVLNDKIHWVTFHCGYDFGYLLKLLSGKELPE 196

Query: 194 SMAAFATTAQRLLGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLL 253
            ++ F    ++    + D+K++  +C  L     GL+K+A L+ VKRVGI+H AGSDSLL
Sbjct: 197 EISDFFDQMEKFFPVVYDIKYLMGFCTNLYG---GLEKIAELLGVKRVGISHQAGSDSLL 256

Query: 254 TASVYAKMK-LMFKLNPEQCEGFLYGL 260
           T   + KMK   F  +  +  GFL+GL
Sbjct: 257 TLRTFIKMKEFFFTGSLLKYSGFLFGL 275

BLAST of Sed0014532 vs. ExPASy Swiss-Prot
Match: Q9SKZ2 (Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=CAF1-7 PE=2 SV=2)

HSP 1 Score: 180.3 bits (456), Expect = 3.5e-44
Identity = 108/263 (41.06%), Postives = 154/263 (58.56%), Query Frame = 0

Query: 14  REVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYTPKG---VSEEEFYECLRFNVNR 73
           REVW+ NL  E+ LI+E + +FP VA+DTEFPG +   P G    + E  YE L+ NVN 
Sbjct: 13  REVWNDNLESEMALIREVVDDFPFVAMDTEFPGIV-CRPVGTFKTNTEYHYETLKTNVNI 72

Query: 74  LSILQLGLTLTDDKGRIGPT---------WEFNFRDFDLISDMHGVSSLDSFLKNKGFDF 133
           L ++QLGLT +D+KG + PT         W+FNFR+FDL SD++   S++  L+  G DF
Sbjct: 73  LKMIQLGLTFSDEKGNL-PTCGTDNKYCIWQFNFREFDLESDIYATDSIE-LLRQSGIDF 132

Query: 134 EKLRKDGIAPEEFSAMFLPICRSGRV----DKWVTFHGFYDIAYLMKLLKIRFIATSMAA 193
            K  + GI  + F+ + +    SG V      WVTFH  YD  YL+KLL  + +  +   
Sbjct: 133 VKNNEFGIDSKRFAELLM---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPETQTG 192

Query: 194 FATTAQRLLGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLLTASV 253
           F          + D+KH+ ++C+ L     GL KLA L++V+RVGI H AGSDSLLT+  
Sbjct: 193 FFEMISVYFPRVYDIKHLMKFCNSLHG---GLNKLAELLDVERVGICHQAGSDSLLTSCT 252

Query: 254 YAKMKLMFKL-NPEQCEGFLYGL 260
           + K++  F + + E+  G LYGL
Sbjct: 253 FRKLQENFFIGSMEKYSGVLYGL 266

BLAST of Sed0014532 vs. ExPASy Swiss-Prot
Match: Q9LXM2 (Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=CAF1-9 PE=2 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.3e-43
Identity = 105/267 (39.33%), Postives = 154/267 (57.68%), Query Frame = 0

Query: 14  REVWSHNLREEIELIKEYLPEFPIVAIDTEFPGF-----LRYTPKGVSEEEFYECLRFNV 73
           REVW+ NL  E ELI E + ++P +++DTEFPG      LR+T    + ++ Y  L+ NV
Sbjct: 20  REVWAENLESEFELISEIIDDYPFISMDTEFPGVIFKSDLRFT----NPDDLYTLLKANV 79

Query: 74  NRLSILQLGLTLTDDKGRI---------GPTWEFNFRDFDLISDMHGVSSLDSFLKNKGF 133
           + LS++Q+GLTL+D  G +         G  WEFNFRDFD+  D H   S++  L+ +G 
Sbjct: 80  DALSLIQVGLTLSDVNGNLPDLGDDLHRGFIWEFNFRDFDVARDAHAPDSIE-LLRRQGI 139

Query: 134 DFEKLRKDGIAPEEFSAMFLP---ICRSGRVDKWVTFHGFYDIAYLMKLLKIRFIATSMA 193
           DFE+  +DG+  E F+ + +    +C       WVTFH  YD  YLMK+L  R +  ++ 
Sbjct: 140 DFERNCRDGVESERFAELMMSSGLVC--NEEVSWVTFHSAYDFGYLMKILTRRELPGALG 199

Query: 194 AFATTAQRLLG-TIVDLKHMARYCHGLLNGDLGLKKLARLVNVKR-VGIAHFAGSDSLLT 253
            F    + L G  + D+KHM ++C   L G  GL ++AR + V R VG  H AGSDSLLT
Sbjct: 200 EFKRVMRVLFGERVYDVKHMMKFCERRLFG--GLDRVARTLEVNRAVGKCHQAGSDSLLT 259

Query: 254 ASVYAKMKLMFKL--NPEQCEGFLYGL 260
              + +M+ ++ +   PE+  G LYGL
Sbjct: 260 WHAFQRMRDLYFVQDGPEKHAGVLYGL 277

BLAST of Sed0014532 vs. ExPASy Swiss-Prot
Match: Q9LXM4 (Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=CAF1-8 PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 5.5e-42
Identity = 99/224 (44.20%), Postives = 136/224 (60.71%), Query Frame = 0

Query: 25  IELIKEYLPEFPIVAIDTEFPGFLRYTPKGVSEEEFYECLRFNVNRLSILQLGLTLTDDK 84
           + LI++ L  +  +AIDTEFP  LR T +  ++EE Y  + F+V+R  ++QLGLTL D  
Sbjct: 1   MSLIEDCLRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDIN 60

Query: 85  GRIGPTWEFNFRDFDLISDMHGVSSLDSFLKNKGFDFEKLRKDGIAPEE-FSAMFLPICR 144
           GRIG TWE NF DF  + D     S++ FL+  G D  K+R++GI  E  FS MF  + +
Sbjct: 61  GRIGGTWEINFSDFG-VDDARNEKSIE-FLRRNGLDLRKIREEGIRIEGFFSEMFWMLKK 120

Query: 145 SGRVDKWVTFHGFYDIAYLMKLLKIRFIATSMAAFATTAQRLLGTIVDLKHMARYCHGLL 204
           + R   WVTFHG YDIAYL+K      +  +   F+    R+LG++ DLK MA  C G L
Sbjct: 121 TRRNITWVTFHGSYDIAYLLKGFTGEALPVTSERFSKAVARVLGSVYDLKVMAGRCEG-L 180

Query: 205 NGDLGLKKLARLVNVKRVGIAHFAGSDSLLTASVYAKMKLMFKL 248
           +  LGL+ LA    + RVG AH AGS++ LTA V+AK+   F L
Sbjct: 181 SSRLGLETLAHEFGLNRVGTAHHAGSNNELTAMVFAKVLSPFPL 221

BLAST of Sed0014532 vs. ExPASy TrEMBL
Match: A0A6J1DLA5 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=3 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 6.2e-113
Identity = 205/310 (66.13%), Postives = 241/310 (77.74%), Query Frame = 0

Query: 2   KNTSAKISSSFNREVWSHNLREEIELIKEYLPEFPIVAIDTEF----------------- 61
           K+ +A  S   NREVWS+NL +E+E++ EYLPEFPIVAIDTEF                 
Sbjct: 7   KSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRIYFLSLA 66

Query: 62  ------------PGFLRYTPKGVSEEEFYECLRFNVNRLSILQLGLTLTDDKGRIGPTWE 121
                       PGFLRYTPKGVSEEEFY+CLRFNVNRL+ILQLGLTL +  G +  TWE
Sbjct: 67  GKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNVAMTWE 126

Query: 122 FNFRDFDLISDMHGVSSLDSFLKNKGFDFEKLRKDGIAPEEFSAMFLPICRSGRVDKWVT 181
           FNFRDFD ISDM GVSSLDSFLKNKGFDF KL++DGIA E+F+ +FLPICRSGR+++W+T
Sbjct: 127 FNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGRIERWIT 186

Query: 182 FHGFYDIAYLMKLLKIRFIATSMAAFATTAQRLLGTIVDLKHMARYCHGLLNGDLGLKKL 241
           FHGFYDIAYL+KLLKI++I  SMA FA TAQ LLGT+ DLKHM RYC GLL+GDLGLKKL
Sbjct: 187 FHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGDLGLKKL 246

Query: 242 ARLVNVKRVGIAHFAGSDSLLTASVYAKMKLMFKLNPEQCEGFLYGLPHSIRSTRHTVKV 283
           A+L++VKR+GIAHFAGSDSLLTA+VY KMK MFK+  EQ EGFLYGLPH I++ +  VK+
Sbjct: 247 AKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQMFKVPVEQREGFLYGLPHRIQAFQPIVKI 306

BLAST of Sed0014532 vs. ExPASy TrEMBL
Match: A0A6J1CXZ5 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015612 PE=3 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 2.4e-112
Identity = 207/307 (67.43%), Postives = 241/307 (78.50%), Query Frame = 0

Query: 2   KNTSAKISSSFNREVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYTPKGVSEEEFY 61
           K+T+A  S   NREVWS NLREE+EL+ EYLPEFP+V ID EFPGFL  TPKG+SEEEFY
Sbjct: 26  KSTAAPRSICHNREVWSLNLREELELLNEYLPEFPVVVIDMEFPGFLWCTPKGISEEEFY 85

Query: 62  ECLRFNVNRLSILQLGLTLTDDKGRIGPTWEFNFRDFDLISDMHGVSSLDSFLKNKGFDF 121
            CLRFNVNRL+ILQLGLTL D KG I  TWEFNFRDFD +SDM  VSSLDSFLKNKGFDF
Sbjct: 86  HCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDF 145

Query: 122 EKLRKDGIAPEEFSAMFLPICRSGRVDKWVTFHGFYDIAYLMKLLKIRFIATSMAAFATT 181
            KL+K GIA E+F+ +FLPICRSG++ +W+TFHGFYDI YL+KLLKI+ I  SMA FATT
Sbjct: 146 YKLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGFYDITYLLKLLKIKSIPISMATFATT 205

Query: 182 AQRLLGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLLTASVYAKM 241
           AQ LLGT+ DLKHMARYC GLL+G+LGLKKLA+L++VKR+  AHFA SD+L T SVY KM
Sbjct: 206 AQHLLGTVSDLKHMARYCDGLLDGNLGLKKLAKLLDVKRIDNAHFACSDNLFTTSVYTKM 265

Query: 242 KLMFKLNPEQCEGFLYGLPHSIRSTRHTVKVIDPSTATSSPML--KYPSMPQCSSASAYR 301
           K +FKL  EQCEGFLYGLP+ I   +   K+IDPST T  P L  K+  +  CS      
Sbjct: 266 KQIFKLPLEQCEGFLYGLPYPINGFQSVAKIIDPSTMTPPPPLPSKFSILSFCS------ 325

Query: 302 SSPIFVG 307
           S+P+ VG
Sbjct: 326 STPLLVG 326

BLAST of Sed0014532 vs. ExPASy TrEMBL
Match: A0A6J1ESM7 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 5.6e-106
Identity = 191/275 (69.45%), Postives = 222/275 (80.73%), Query Frame = 0

Query: 5   SAKISSSFNREVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYTPKGVSEEEFYECL 64
           +A +   + REVWSHNL EEIEL+K YL EFPIVAIDTEFPGFLR+TPKGVS+EEFY+CL
Sbjct: 8   AAIVGGQWKREVWSHNLLEEIELMKHYLSEFPIVAIDTEFPGFLRWTPKGVSDEEFYQCL 67

Query: 65  RFNVNRLSILQLGLTLTDDKGRIGPTWEFNFRDFDLISDMHGVSSLDSFLKNKGFDFEKL 124
           +FNVNRLSILQLGLTLTDD G+IG TWEFNF+DFD IS+M GV SLDSFLKNKGF+F KL
Sbjct: 68  QFNVNRLSILQLGLTLTDDNGKIGMTWEFNFQDFDQISNMEGVYSLDSFLKNKGFNFNKL 127

Query: 125 RKDGIAPEEFSAMFLPICRSGRVDKWVTFHGFYDIAYLMKLLKIRFIATSMAAFATTAQR 184
           R  GIAP+EFS  F P+ RSGR+ KW+TFHG YDIAYLMK   I  + TSM+ FA  A  
Sbjct: 128 RSCGIAPQEFSRQFKPVIRSGRIQKWITFHGLYDIAYLMKFNNIISMPTSMSMFAMIAAH 187

Query: 185 LLGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLLTASVYAKMKLM 244
           LLGT+ DLKHMARY   L NG+LGLKKLA+L+ + RVG AHFAGSDSLLTASVYAKMK++
Sbjct: 188 LLGTVTDLKHMARYSDYLFNGNLGLKKLAKLLQINRVGTAHFAGSDSLLTASVYAKMKVI 247

Query: 245 FKLNPEQCEGFLYGLPHSIRSTRHTVKVIDPSTAT 280
            K+ P+  EGFLYGLP+ I+  R  V ++DPS  T
Sbjct: 248 MKVEPKIHEGFLYGLPYPIQGYRPPVVIVDPSKLT 282

BLAST of Sed0014532 vs. ExPASy TrEMBL
Match: A0A6J1HL76 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463977 PE=3 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 9.0e-104
Identity = 190/274 (69.34%), Postives = 219/274 (79.93%), Query Frame = 0

Query: 6   AKISSSFNREVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYTPKGVSEEEFYECLR 65
           A +   + R+VWSHNL EEIELIK+ L EFPIVAIDTEFPGFLR+TPKGVS+EEFYECL+
Sbjct: 9   AIVEGQWKRDVWSHNLLEEIELIKQCLSEFPIVAIDTEFPGFLRWTPKGVSDEEFYECLQ 68

Query: 66  FNVNRLSILQLGLTLTDDKGRIGPTWEFNFRDFDLISDMHGVSSLDSFLKNKGFDFEKLR 125
           FNVNRLSILQLGLTLT+D G+IG TWEFNFRDFD IS+M GV SLDSFLKNKGF+F  LR
Sbjct: 69  FNVNRLSILQLGLTLTNDNGKIGMTWEFNFRDFDKISNMEGVYSLDSFLKNKGFNFNTLR 128

Query: 126 KDGIAPEEFSAMFLPICRSGRVDKWVTFHGFYDIAYLMKLLKIRFIATSMAAFATTAQRL 185
             GIAPEEF   F PI RSGR+ +W+TFHG YDIAYLMK   IR + TSM+ FA TA  +
Sbjct: 129 SYGIAPEEFGTQFSPILRSGRIHRWITFHGLYDIAYLMKFTNIRCMPTSMSIFAITAAHI 188

Query: 186 LGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLLTASVYAKMKLMF 245
           LGT+ DLKHMA Y   LL+G+LGLKKLA+L+ V R G AHFAGSDSLLTASVYAKMK++ 
Sbjct: 189 LGTVTDLKHMAWY--SLLDGNLGLKKLAKLLQVNRTGTAHFAGSDSLLTASVYAKMKIIM 248

Query: 246 KLNPEQCEGFLYGLPHSIRSTRHTVKVIDPSTAT 280
           K+ P   EGFLYGLP+ I+  R  V ++DPS  T
Sbjct: 249 KVEPNIHEGFLYGLPYPIQGYRRPVVIVDPSKLT 280

BLAST of Sed0014532 vs. ExPASy TrEMBL
Match: A0A6J1HIZ7 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463978 PE=3 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 5.8e-103
Identity = 187/274 (68.25%), Postives = 219/274 (79.93%), Query Frame = 0

Query: 6   AKISSSFNREVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYTPKGVSEEEFYECLR 65
           A +   + R+VWSHNL EEIELI++ L EFPIVA+DTEFPGFLR+TPKGVS+EEFYECL+
Sbjct: 6   ATVEGQWERDVWSHNLLEEIELIQQCLSEFPIVAMDTEFPGFLRWTPKGVSDEEFYECLQ 65

Query: 66  FNVNRLSILQLGLTLTDDKGRIGPTWEFNFRDFDLISDMHGVSSLDSFLKNKGFDFEKLR 125
           FNVNRLSILQLGLTLT+D G+IG TWEFNF+DFD IS+M GV SLDSFLKNKGF+F  LR
Sbjct: 66  FNVNRLSILQLGLTLTNDNGKIGMTWEFNFKDFDKISNMEGVYSLDSFLKNKGFNFNTLR 125

Query: 126 KDGIAPEEFSAMFLPICRSGRVDKWVTFHGFYDIAYLMKLLKIRFIATSMAAFATTAQRL 185
             GIAPEEF   F PI RSGR+ +W+TFHG YDIAYLMKL  I  + TSM+ FA TA  L
Sbjct: 126 SYGIAPEEFGRQFNPILRSGRIHRWITFHGLYDIAYLMKLTNITCMPTSMSIFAITAAHL 185

Query: 186 LGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLLTASVYAKMKLMF 245
           LGT+ DLKHMAR  + L NG+LGLKKLA+L+ V R G AHFAGSDSLLTASVYAKMK++ 
Sbjct: 186 LGTVTDLKHMARCSNYLFNGNLGLKKLAKLLQVNRTGTAHFAGSDSLLTASVYAKMKIIM 245

Query: 246 KLNPEQCEGFLYGLPHSIRSTRHTVKVIDPSTAT 280
           K+  +  EGFLYGLP+ I+  R  V ++DPS  T
Sbjct: 246 KVEQKIDEGFLYGLPYPIQGYRRPVVIVDPSKLT 279

BLAST of Sed0014532 vs. TAIR 10
Match: AT1G80780.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 183.3 bits (464), Expect = 2.9e-46
Identity = 109/262 (41.60%), Postives = 155/262 (59.16%), Query Frame = 0

Query: 14  REVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYTPKGV---SEEEFYECLRFNVNR 73
           REVW+ NL+EE++LI++ + +FP VA+DTEFPG +   P G    + +  YE L+ NVN 
Sbjct: 13  REVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIV-VRPVGTFKSNADYHYETLKTNVNI 72

Query: 74  LSILQLGLTLTDDKGRIGPT--------WEFNFRDFDLISDMHGVSSLDSFLKNKGFDFE 133
           L ++QLGLT ++++G + PT        W+FNFR+FDL SD+  + S++  LK  G D  
Sbjct: 73  LKMIQLGLTFSNEQGNL-PTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLA 132

Query: 134 KLRKDGIAPEEFSAMFLPICRSGRV----DKWVTFHGFYDIAYLMKLLKIRFIATSMAAF 193
           K   DGI  + F+ + +    SG V      WVTFH  YD  YL+KLL  + +  S   F
Sbjct: 133 KNTLDGIDSKRFAELLM---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDF 192

Query: 194 ATTAQRLLGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLLTASVY 253
                    T+ D+KH+ ++C+ L     GL KLA L+ V+RVGI H AGSDSLLT+  +
Sbjct: 193 FKLINVYFPTVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTF 252

Query: 254 AKMKLMFKLNP-EQCEGFLYGL 260
            K+K  F + P  +  G LYGL
Sbjct: 253 RKLKENFFVGPLHKYSGVLYGL 265

BLAST of Sed0014532 vs. TAIR 10
Match: AT1G80780.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 183.3 bits (464), Expect = 2.9e-46
Identity = 109/262 (41.60%), Postives = 155/262 (59.16%), Query Frame = 0

Query: 14  REVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYTPKGV---SEEEFYECLRFNVNR 73
           REVW+ NL+EE++LI++ + +FP VA+DTEFPG +   P G    + +  YE L+ NVN 
Sbjct: 13  REVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIV-VRPVGTFKSNADYHYETLKTNVNI 72

Query: 74  LSILQLGLTLTDDKGRIGPT--------WEFNFRDFDLISDMHGVSSLDSFLKNKGFDFE 133
           L ++QLGLT ++++G + PT        W+FNFR+FDL SD+  + S++  LK  G D  
Sbjct: 73  LKMIQLGLTFSNEQGNL-PTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLA 132

Query: 134 KLRKDGIAPEEFSAMFLPICRSGRV----DKWVTFHGFYDIAYLMKLLKIRFIATSMAAF 193
           K   DGI  + F+ + +    SG V      WVTFH  YD  YL+KLL  + +  S   F
Sbjct: 133 KNTLDGIDSKRFAELLM---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDF 192

Query: 194 ATTAQRLLGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLLTASVY 253
                    T+ D+KH+ ++C+ L     GL KLA L+ V+RVGI H AGSDSLLT+  +
Sbjct: 193 FKLINVYFPTVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTF 252

Query: 254 AKMKLMFKLNP-EQCEGFLYGL 260
            K+K  F + P  +  G LYGL
Sbjct: 253 RKLKENFFVGPLHKYSGVLYGL 265

BLAST of Sed0014532 vs. TAIR 10
Match: AT1G80780.3 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 183.3 bits (464), Expect = 2.9e-46
Identity = 109/262 (41.60%), Postives = 155/262 (59.16%), Query Frame = 0

Query: 14  REVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYTPKGV---SEEEFYECLRFNVNR 73
           REVW+ NL+EE++LI++ + +FP VA+DTEFPG +   P G    + +  YE L+ NVN 
Sbjct: 13  REVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIV-VRPVGTFKSNADYHYETLKTNVNI 72

Query: 74  LSILQLGLTLTDDKGRIGPT--------WEFNFRDFDLISDMHGVSSLDSFLKNKGFDFE 133
           L ++QLGLT ++++G + PT        W+FNFR+FDL SD+  + S++  LK  G D  
Sbjct: 73  LKMIQLGLTFSNEQGNL-PTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLA 132

Query: 134 KLRKDGIAPEEFSAMFLPICRSGRV----DKWVTFHGFYDIAYLMKLLKIRFIATSMAAF 193
           K   DGI  + F+ + +    SG V      WVTFH  YD  YL+KLL  + +  S   F
Sbjct: 133 KNTLDGIDSKRFAELLM---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDF 192

Query: 194 ATTAQRLLGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLLTASVY 253
                    T+ D+KH+ ++C+ L     GL KLA L+ V+RVGI H AGSDSLLT+  +
Sbjct: 193 FKLINVYFPTVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTF 252

Query: 254 AKMKLMFKLNP-EQCEGFLYGL 260
            K+K  F + P  +  G LYGL
Sbjct: 253 RKLKENFFVGPLHKYSGVLYGL 265

BLAST of Sed0014532 vs. TAIR 10
Match: AT1G15920.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 183.0 bits (463), Expect = 3.8e-46
Identity = 108/267 (40.45%), Postives = 159/267 (59.55%), Query Frame = 0

Query: 14  REVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYT--------PKGVSEEEFYECLR 73
           REVW+HNL +E+ LI++ + +FP VA+DTEFPG +  T        P  +  E  Y+ L+
Sbjct: 17  REVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGIVCKTVTANPNPNPYSIHYEYNYDTLK 76

Query: 74  FNVNRLSILQLGLTLTDDKGRIGPT--------WEFNFRDFDLISDMHGVSSLDSFLKNK 133
            NVN L ++QLGLTL+D+KG + PT        W+FNFR+F++ISDM  + S++  L+  
Sbjct: 77  ANVNMLKLIQLGLTLSDEKGNL-PTCGTNKQCIWQFNFREFNVISDMFALDSIE-LLRKS 136

Query: 134 GFDFEKLRKDGIAPEEFSAMFLPICRSGRV--DK--WVTFHGFYDIAYLMKLLKIRFIAT 193
             D EK  + G+  + F+ + +    SG V  DK  WVTFH  YD  YL+KLL  + +  
Sbjct: 137 AIDLEKNNECGVDAKRFAELLM---GSGVVLNDKIHWVTFHCGYDFGYLLKLLSGKELPE 196

Query: 194 SMAAFATTAQRLLGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLL 253
            ++ F    ++    + D+K++  +C  L     GL+K+A L+ VKRVGI+H AGSDSLL
Sbjct: 197 EISDFFDQMEKFFPVVYDIKYLMGFCTNLYG---GLEKIAELLGVKRVGISHQAGSDSLL 256

Query: 254 TASVYAKMK-LMFKLNPEQCEGFLYGL 260
           T   + KMK   F  +  +  GFL+GL
Sbjct: 257 TLRTFIKMKEFFFTGSLLKYSGFLFGL 275

BLAST of Sed0014532 vs. TAIR 10
Match: AT1G15920.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 183.0 bits (463), Expect = 3.8e-46
Identity = 108/267 (40.45%), Postives = 159/267 (59.55%), Query Frame = 0

Query: 14  REVWSHNLREEIELIKEYLPEFPIVAIDTEFPGFLRYT--------PKGVSEEEFYECLR 73
           REVW+HNL +E+ LI++ + +FP VA+DTEFPG +  T        P  +  E  Y+ L+
Sbjct: 17  REVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGIVCKTVTANPNPNPYSIHYEYNYDTLK 76

Query: 74  FNVNRLSILQLGLTLTDDKGRIGPT--------WEFNFRDFDLISDMHGVSSLDSFLKNK 133
            NVN L ++QLGLTL+D+KG + PT        W+FNFR+F++ISDM  + S++  L+  
Sbjct: 77  ANVNMLKLIQLGLTLSDEKGNL-PTCGTNKQCIWQFNFREFNVISDMFALDSIE-LLRKS 136

Query: 134 GFDFEKLRKDGIAPEEFSAMFLPICRSGRV--DK--WVTFHGFYDIAYLMKLLKIRFIAT 193
             D EK  + G+  + F+ + +    SG V  DK  WVTFH  YD  YL+KLL  + +  
Sbjct: 137 AIDLEKNNECGVDAKRFAELLM---GSGVVLNDKIHWVTFHCGYDFGYLLKLLSGKELPE 196

Query: 194 SMAAFATTAQRLLGTIVDLKHMARYCHGLLNGDLGLKKLARLVNVKRVGIAHFAGSDSLL 253
            ++ F    ++    + D+K++  +C  L     GL+K+A L+ VKRVGI+H AGSDSLL
Sbjct: 197 EISDFFDQMEKFFPVVYDIKYLMGFCTNLYG---GLEKIAELLGVKRVGISHQAGSDSLL 256

Query: 254 TASVYAKMK-LMFKLNPEQCEGFLYGL 260
           T   + KMK   F  +  +  GFL+GL
Sbjct: 257 TLRTFIKMKEFFFTGSLLKYSGFLFGL 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154798.11.3e-11266.13probable CCR4-associated factor 1 homolog 6 [Momordica charantia][more]
XP_022146384.14.9e-11267.43putative CCR4-associated factor 1 homolog 8 [Momordica charantia][more]
XP_022928830.11.2e-10569.45putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata][more]
XP_023511526.13.7e-10467.52putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo][more]
XP_022963784.11.9e-10369.34putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SAI24.1e-4541.60Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9S9P25.3e-4540.45Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9SKZ23.5e-4441.06Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9LXM21.3e-4339.33Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9LXM45.5e-4244.20Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Match NameE-valueIdentityDescription
A0A6J1DLA56.2e-11366.13Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=... [more]
A0A6J1CXZ52.4e-11267.43Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015612 PE=... [more]
A0A6J1ESM75.6e-10669.45Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3... [more]
A0A6J1HL769.0e-10469.34Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463977 PE=3... [more]
A0A6J1HIZ75.8e-10368.25Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463978 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G80780.12.9e-4641.60Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G80780.22.9e-4641.60Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G80780.32.9e-4641.60Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G15920.23.8e-4640.45Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G15920.13.8e-4640.45Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 3..262
e-value: 3.9E-71
score: 241.3
IPR006941Ribonuclease CAF1PFAMPF04857CAF1coord: 16..129
e-value: 3.2E-9
score: 36.5
NoneNo IPR availablePANTHERPTHR10797:SF57CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATEDcoord: 13..270
IPR039637CCR4-NOT transcription complex subunit 7/8/Pop2PANTHERPTHR10797CCR4-NOT TRANSCRIPTION COMPLEX SUBUNITcoord: 13..270
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 7..255

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0014532.1Sed0014532.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006402 mRNA catabolic process
biological_process GO:0090503 RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component GO:0030014 CCR4-NOT complex
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004535 poly(A)-specific ribonuclease activity