Sed0013457 (gene) Chayote v1

Overview
NameSed0013457
Typegene
OrganismSechium edule (Chayote v1)
DescriptionCytochrome P450 81E8-like
LocationLG01: 21603718 .. 21607256 (-)
RNA-Seq ExpressionSed0013457
SyntenySed0013457
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAAATAGAGAAACTATCATGGCTCACCCATTTCCTCATCTCTACCACCCTTCTCTTCATTCTCGTTCTCATTTCAAAATTCAGAAAACCCCATTACAAAAGCCTCCCTCCATCGCCGCCGTACCTTCCGGTCATTGGCCACCTCCATCTTCTCAAGAACCCATTCCACAGAGTCCTCCAAAATCTCTCCCAAAAACTCGGCCCCATCTTCTTCCTCTCCATCGGCTCCCGTTCCGTCGTCGTGATCTCCTCCCCGGCCGCCGCCCAGCAATGCTTCACCAAAAACGACATCGTTTTGGCGAACCGCCCCCGCCTCCTGGCCGGAAAGTACATCAACTACAACTACACCGGAGTGGCGTTCGCCGCTTACGGCCACCACTGGCGCAACATGCGGCGCATCGCCACCACGGAGCTCCTCTCCAACCACCGCCTCGGCAATGACCTCAGCGTCCGAGCCCACGAGCTCAAGCTGTGGCTGAAGGATCTGTACAACGAAACAGGGGAAAACAGGGGATTTGTTTGTGTGGAAATGAAGGCGAAGTTGAACGAGGTTTCGTTCAATACCGTGATGAGGATGGTAAGTGGGAAGAGGTATTTTGGGGCTGGGGTTGAGAATGGAGAGGAATCGAGGGAATTCAGGGGGATTATGAAGGAGATTGTTGAGCTTAGTGGAGCGTCGAATCCTGTGGATTTTTTGCCCATTTTGAGAATGATTGGTTTCGGGGGGTTGGAGAAGAGGATGATGGAGGCGAGTGAGCGAGCGGATGGGTTTCTACAGAAGCTGATCGATGAAGAAAGAGATAAGAGAGTTTCGCGTTGGCCGGAGGGGAAAGATGGAAACACAATGATTCAGAGTTTGCTCTGTTTGCAACAGTCTGAACCTCAGTATTACACCGATGACATTATAAAGGCTCATGTTCTGGTAAGTTACTACTACTACTATTATTATTATTTGAACAGAGCTGAAATCTGGTAAGTTAAAATAAACATTCTCCTTTTTGGAAAAATTTATAAAATAAGTTACTCAATAAAATAATAGGATAAATTGTCAAATGAGGTCATTTTTTAAAAGTTATTGTTAAAATGGGTCTGATTCCAAATTATTTTTAAAAATGGGTTGTGGCGTCTGTGTAAGAGACGCCACACTAGTTATTTTCGTTTGGCAAATTTCATGGCCACACACGTGGCAGTGTGGTCAACTTAACAAAAATCTACTACAATTTTGCAGATTTTCTTTCAGTTTTTTTTTTTCTTTTTAATTACAGATTTAATCGTTTTTTCATATAAATATGTTTTTTACGTTTACTTTATCGGTGGTTTCATATTTTCAGGTGTTATTTTATTTCTTTACACTTTAATATCTCATTACTTTCATTTTCTTTAAATTTGTTAAGACATTTTTTTTAATGTTGATACTAATTAATTTTTTTTATAATATTATAATTTAATTAGGATTTAAGAATGCGTATTAATCTATGATCATTGTAACAGTTGCAAAAAATAATTGATTATGTCAAAGGTAGTTGTTAGGGTTATGATGAATGTTGATTAAATAGATATGTGTGAGTTATTTATGATTATTCCAAAGATTGATAAAATGATTTAATGTATATTTGTTAATAAAAAAATTGAACTAAACTTCAAAAAAAAAAAAAACTACAATTTATGATAACATACAATTTTTTCAATATCCTAATAACTTCAATTTTTTTTTTTTTTGAAAATCGGGAAATCAGAACTTCGCTCCCTTATACGCGGTACCACTAAAGAAAAAACATGTGTTGAAGTTTTTATCCTCAATCGCTGAACTTCCAACTCTTCTTTCAAATCTTAACGACCTTAATTTAAATCATTAAAAAACATGCAAACTTATTTGAAATTTAAAAATAAAATAATTAGAAAGAAAAAAGTAGAAGATATTTTATGAGTGGTCAAAAAACAATTCTACCAATATCAAAATTTTATTAAAATATGAAATAAAGTAAAAACGGATTCCTTCGATTGATAGATGAAACTTGAAATGATAGTTTTTAATCAAATTAAATTTGAATTATTTTATTATAAATATAATATTTGTTATAATTAAATTAGGGAGACATATCTGCTGATTCCAAATAGGATATGGCCTTGGCCACTGATTTAATTGGATATATTTGGAATCTGCTGAGGGTATTTGAATTCAAGGTAAAAGTCTTTTAACTTTAAAATTTATTATATTTTGGTGAAGTGGTTTCTCAAAATATATATATATATATATATATATATATATATATATATATATATATTAGTGAAAATTTTTAAAATTTTAATTTTAGTACCTAAAATTTGGAGTTTGTTTTATTTTGATTCATGAACTTAAAAAAAAAAAAAAAATCTATAATATTTGAGACTGAAACAATAACCAATTGATTATTAAATGATACATAATATAATATTAATTGAAATTGAAATTGGTTCAATAATATAAATCATATTAATAAATTTAAGGAGAAAACATGAACAAATATTTAAATTTTACATGGGAAAATGATAATATTTTTTAAAGATAAATAAAGAAAAAATAATTTTAAGGATCAATTTAAAGTTTTTTTAAAGTTTAGAATCGAAATGAAAATAACATGGATTAAATTAAAATTTTGAAAGTTTTAAAACTAAAATGAAACAAATCTTAAATTTATATGAATCAAAATTAAACTTTAAAAATTTAGAAGCTAAATAAAACAAACCTCAATGATGAGGAATCAAAATAAACTTTACCCTTAAATTGTTGAACTCAAATGGAGTTGATTGATATTAAAACTCGAGTAAGTTTTAATAAATTTTTTAAGTTTATGTTTTTCTAATAAGAATATGTTGAACTCAAATGAAATTAAATTATGTTTCTCAAACTTAAATTAATAATATCTGCAGACCATGTTGGTAGCAGGCACTGATACAACAACAGGAACATTAGTCTGGGCAATGTCACTACTTCTGAATCATCCAACCATCATGAACAAGGCTTGGACCGAGATCAGCTAGCACGTCGGTGACAATCGAATAGCGGAGGAAGCCGATATTAGCAAGTTACAATATCTTCAAGCCATAATCTACGAGACTTTTCGACTATATCCAATAGCTCCGCTATTGGTGCCGCATGAAGCCTCAGAAGACTGCACAATTGAAGGATTCGACGTGCCAAAAGGAACAATGCTGCTAGTGAACGCATGGGCCATGCACAGGGATCCGAAGGTGTGGGAGGATCCAACGCGCTTCCGACCAGAGAGGTTTGTTGATTGGGAAGGTAACATTGAGTCGTCGTACAAATGGATGCCGTTTGGAATGGGGAGGAGGGCTTGTCCCGGGGCTGTGCTTGGTCAACGAGTCGTGGGGTTGACTTTGGCGACGTTGATTCAATGCTTTGAGTGGAAGAGAATTGGGGAAGAAGAAGTAGACTTGTCGGAGGGAAATGGAATCACAATGCCCAAAACTACTCCTTTGGAGGCAATGTTTAGAGTTCGTAGCTCCATGGTTCATTTGCTTTCCCAACTTTAA

mRNA sequence

ATGGCGGAAATAGAGAAACTATCATGGCTCACCCATTTCCTCATCTCTACCACCCTTCTCTTCATTCTCGTTCTCATTTCAAAATTCAGAAAACCCCATTACAAAAGCCTCCCTCCATCGCCGCCGTACCTTCCGGTCATTGGCCACCTCCATCTTCTCAAGAACCCATTCCACAGAGTCCTCCAAAATCTCTCCCAAAAACTCGGCCCCATCTTCTTCCTCTCCATCGGCTCCCGTTCCGTCGTCGTGATCTCCTCCCCGGCCGCCGCCCAGCAATGCTTCACCAAAAACGACATCGTTTTGGCGAACCGCCCCCGCCTCCTGGCCGGAAAGTACATCAACTACAACTACACCGGAGTGGCGTTCGCCGCTTACGGCCACCACTGGCGCAACATGCGGCGCATCGCCACCACGGAGCTCCTCTCCAACCACCGCCTCGGCAATGACCTCAGCGTCCGAGCCCACGAGCTCAAGCTGTGGCTGAAGGATCTGTACAACGAAACAGGGGAAAACAGGGGATTTGTTTCGTTCAATACCGTGATGAGGATGGTAAGTGGGAAGAGGTATTTTGGGGCTGGGGTTGAGAATGGAGAGGAATCGAGGGAATTCAGGGGGATTATGAAGGAGATTGTTGAGCTTAGTGGAGCGTCGAATCCTGTGGATTTTTTGCCCATTTTGAGAATGATTGGTTTCGGGGGGTTGGAGAAGAGGATGATGGAGGCGAGTGAGCGAGCGGATGGGTTTCTACAGAAGCTGATCGATGAAGAAAGAGATAAGAGAGTTTCGCGTTGGCCGGAGGGGAAAGATGGAAACACAATGATTCAGAGTTTGCTCTGTTTGCAACAGTCTGAACCTCAGTATTACACCGATGACATTATAAAGGCTCATGTTCTGACCATGTTGGTAGCAGGCACTGATACAACAACAGGAACATTAGTCTGGGCAATGTCACTACTTCTGAATCATCCAACCATCATGAACAAGGCTTGGACCGAGATCAGCTACAAGTTACAATATCTTCAAGCCATAATCTACGAGACTTTTCGACTATATCCAATAGCTCCGCTATTGGTGCCGCATGAAGCCTCAGAAGACTGCACAATTGAAGGATTCGACGTGCCAAAAGGAACAATGCTGCTAGTGAACGCATGGGCCATGCACAGGGATCCGAAGGTGTGGGAGGATCCAACGCGCTTCCGACCAGAGAGGTTTGTTGATTGGGAAGGTAACATTGAGTCGTCGTACAAATGGATGCCGTTTGGAATGGGGAGGAGGGCTTGTCCCGGGGCTGTGCTTGGTCAACGAGTCGTGGGGTTGACTTTGGCGACGTTGATTCAATGCTTTGAGTGGAAGAGAATTGGGGAAGAAGAAGTAGACTTGTCGGAGGGAAATGGAATCACAATGCCCAAAACTACTCCTTTGGAGGCAATGTTTAGAGTTCGTAGCTCCATGGTTCATTTGCTTTCCCAACTTTAA

Coding sequence (CDS)

ATGGCGGAAATAGAGAAACTATCATGGCTCACCCATTTCCTCATCTCTACCACCCTTCTCTTCATTCTCGTTCTCATTTCAAAATTCAGAAAACCCCATTACAAAAGCCTCCCTCCATCGCCGCCGTACCTTCCGGTCATTGGCCACCTCCATCTTCTCAAGAACCCATTCCACAGAGTCCTCCAAAATCTCTCCCAAAAACTCGGCCCCATCTTCTTCCTCTCCATCGGCTCCCGTTCCGTCGTCGTGATCTCCTCCCCGGCCGCCGCCCAGCAATGCTTCACCAAAAACGACATCGTTTTGGCGAACCGCCCCCGCCTCCTGGCCGGAAAGTACATCAACTACAACTACACCGGAGTGGCGTTCGCCGCTTACGGCCACCACTGGCGCAACATGCGGCGCATCGCCACCACGGAGCTCCTCTCCAACCACCGCCTCGGCAATGACCTCAGCGTCCGAGCCCACGAGCTCAAGCTGTGGCTGAAGGATCTGTACAACGAAACAGGGGAAAACAGGGGATTTGTTTCGTTCAATACCGTGATGAGGATGGTAAGTGGGAAGAGGTATTTTGGGGCTGGGGTTGAGAATGGAGAGGAATCGAGGGAATTCAGGGGGATTATGAAGGAGATTGTTGAGCTTAGTGGAGCGTCGAATCCTGTGGATTTTTTGCCCATTTTGAGAATGATTGGTTTCGGGGGGTTGGAGAAGAGGATGATGGAGGCGAGTGAGCGAGCGGATGGGTTTCTACAGAAGCTGATCGATGAAGAAAGAGATAAGAGAGTTTCGCGTTGGCCGGAGGGGAAAGATGGAAACACAATGATTCAGAGTTTGCTCTGTTTGCAACAGTCTGAACCTCAGTATTACACCGATGACATTATAAAGGCTCATGTTCTGACCATGTTGGTAGCAGGCACTGATACAACAACAGGAACATTAGTCTGGGCAATGTCACTACTTCTGAATCATCCAACCATCATGAACAAGGCTTGGACCGAGATCAGCTACAAGTTACAATATCTTCAAGCCATAATCTACGAGACTTTTCGACTATATCCAATAGCTCCGCTATTGGTGCCGCATGAAGCCTCAGAAGACTGCACAATTGAAGGATTCGACGTGCCAAAAGGAACAATGCTGCTAGTGAACGCATGGGCCATGCACAGGGATCCGAAGGTGTGGGAGGATCCAACGCGCTTCCGACCAGAGAGGTTTGTTGATTGGGAAGGTAACATTGAGTCGTCGTACAAATGGATGCCGTTTGGAATGGGGAGGAGGGCTTGTCCCGGGGCTGTGCTTGGTCAACGAGTCGTGGGGTTGACTTTGGCGACGTTGATTCAATGCTTTGAGTGGAAGAGAATTGGGGAAGAAGAAGTAGACTTGTCGGAGGGAAATGGAATCACAATGCCCAAAACTACTCCTTTGGAGGCAATGTTTAGAGTTCGTAGCTCCATGGTTCATTTGCTTTCCCAACTTTAA

Protein sequence

MAEIEKLSWLTHFLISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQNLSQKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFVSFNTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDIIKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEISYKLQYLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL
Homology
BLAST of Sed0013457 vs. NCBI nr
Match: XP_023535658.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 710.7 bits (1833), Expect = 8.9e-201
Identity = 356/515 (69.13%), Postives = 410/515 (79.61%), Query Frame = 0

Query: 3   EIEKLSWLTHFLISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQ 62
           E   ++WL     S +LLF L+LI K RK   +  PPSPP LP+IGHLHLLK PFHRVLQ
Sbjct: 21  EASTITWL-----SLSLLFTLILIHKLRKQSPRKCPPSPPSLPLIGHLHLLKQPFHRVLQ 80

Query: 63  NLSQKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAF 122
           +LS K GPI  LSIGSR +VVISSP A ++CFTKNDI  ANRPRLL+GKY+NYNYTGVAF
Sbjct: 81  DLSNKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGVAF 140

Query: 123 AAYGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV------- 182
           A+YGHHWRNMRRIATTELLSNHRL   L++R  ELKLW+K+LY  TGE+  FV       
Sbjct: 141 ASYGHHWRNMRRIATTELLSNHRLNTYLNIRVQELKLWVKNLYGATGESSDFVRLEMQSK 200

Query: 183 ----SFNTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGF 242
               SFNTVMRMVSGKRYFG  VE+ EE+REFR IMKE+++LSGASNP DFLPILR+  +
Sbjct: 201 LNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLSGASNPADFLPILRLFDY 260

Query: 243 GGLEKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGN-TMIQSLLCLQQSEPQYYTD 302
            GLEKRM++AS RAD FLQ LID ER++R S WPE K GN TMIQSLL LQ+S+PQYY+D
Sbjct: 261 QGLEKRMVKASGRADLFLQNLIDCERERRASPWPEEKHGNKTMIQSLLSLQESQPQYYSD 320

Query: 303 DIIKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YK 362
           DIIK HVLTML AGTDTTTGT+ WAMSLLLNHPTIMNKAW+EI                 
Sbjct: 321 DIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMNKAWSEIRECVGESRLVEEGDVSS 380

Query: 363 LQYLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDP 422
           L+Y++AIIYET RL+P+APLLVPHE+SE+C IEGFD+PKGTMLLVNAWA+HRDP++WEDP
Sbjct: 381 LKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWEDP 440

Query: 423 TRFRPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEE 482
           T FRPER ++WE     S+KW+PFG+GRRACPGA L  RVVGLTLATLIQCFEW+R+GEE
Sbjct: 441 TSFRPERLLNWES--AESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEE 500

Query: 483 EVDLSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
            VDLSEG GIT+PK  PLEAM R RSSM+HLLS L
Sbjct: 501 PVDLSEGTGITIPKAIPLEAMCRARSSMLHLLSLL 528

BLAST of Sed0013457 vs. NCBI nr
Match: XP_022936846.1 (isoflavone 3'-hydroxylase-like isoform X1 [Cucurbita moschata] >XP_022936847.1 isoflavone 3'-hydroxylase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 704.9 bits (1818), Expect = 4.9e-199
Identity = 354/515 (68.74%), Postives = 407/515 (79.03%), Query Frame = 0

Query: 3   EIEKLSWLTHFLISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQ 62
           E   ++WL     S +LLF L LI K RK   +  PPSPP LP+IGHLHLLK PFHRVLQ
Sbjct: 2   EANTITWL-----SLSLLFTLSLIYKLRKQSPRRCPPSPPSLPLIGHLHLLKQPFHRVLQ 61

Query: 63  NLSQKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAF 122
           +LS K GPI  LSIGSR +VVISSP A ++CFTKNDI  ANRPRLL+GKY+NYNYTGVA 
Sbjct: 62  DLSDKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGVAV 121

Query: 123 AAYGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV------- 182
           A+YGHHWRNMRRIATTELLSNHRL   L++R  ELKLW+K+LY ETGE+  FV       
Sbjct: 122 ASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWVKNLYGETGESSDFVRLEMQSK 181

Query: 183 ----SFNTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGF 242
               SFNTVMRMVSGKRYFG  VE+ EE+REFR IMKE++ LSGASNP DFLPILR+  +
Sbjct: 182 LNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLHLSGASNPADFLPILRLFDY 241

Query: 243 GGLEKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGN-TMIQSLLCLQQSEPQYYTD 302
            GLEKRM++AS RAD FLQ LID ER++R SRWPE K GN TMIQSLL  Q+S+PQYY+D
Sbjct: 242 QGLEKRMVKASGRADLFLQNLIDCERERRASRWPEEKHGNKTMIQSLLSFQESQPQYYSD 301

Query: 303 DIIKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YK 362
           DIIK HVLTML AGTDTTTGT+ WAMSLLLNHPTIM+KAW+EI                 
Sbjct: 302 DIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMDKAWSEIRECVGESRLVEEGDVSS 361

Query: 363 LQYLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDP 422
           L+Y++AIIYET RL+P+APLLVPHE+SE+C IEGFD+PKGTMLLVNAWA+HRDP++WEDP
Sbjct: 362 LKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWEDP 421

Query: 423 TRFRPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEE 482
           T FRPERF++WE     S+KW+PFG+GRRACPG  L  RVVGLTLATLIQCFEW+R+GEE
Sbjct: 422 TSFRPERFLNWEA--AESHKWVPFGVGRRACPGVALAHRVVGLTLATLIQCFEWQRVGEE 481

Query: 483 EVDLSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
            +DLSEG GITMPK  PL AM R RSSM+HLLS L
Sbjct: 482 PIDLSEGTGITMPKAIPLVAMCRARSSMLHLLSPL 509

BLAST of Sed0013457 vs. NCBI nr
Match: XP_022977005.1 (isoflavone 3'-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 698.4 bits (1801), Expect = 4.5e-197
Identity = 352/503 (69.98%), Postives = 402/503 (79.92%), Query Frame = 0

Query: 15  ISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQNLSQKLGPIFFL 74
           +S +LL  L+LI K RK   ++ PPSPP LP+IGHLHLLK PFHRVLQ+LS K GPI  L
Sbjct: 20  LSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQDLSNKHGPILSL 79

Query: 75  SIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHHWRNMRR 134
           SIGSR +VVISSP A ++CFTKNDI LANRPRLLAGKY+NYNYTGVAFA+YGHHWRNMRR
Sbjct: 80  SIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFASYGHHWRNMRR 139

Query: 135 IATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV-----------SFNTVMRM 194
           IATTELLSNHRL   L++R  ELKL +K+LY  TGE+  FV           SFN VMRM
Sbjct: 140 IATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFVRLEMQSKLNELSFNIVMRM 199

Query: 195 VSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKRMMEASE 254
           VSGKRYFG  VE+ EE+REFR IMKE+++L GASNP DFLPILR+  + GLEKRM++AS 
Sbjct: 200 VSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQGLEKRMVQASG 259

Query: 255 RADGFLQKLIDEERDKRVSRWPEGKDGN-TMIQSLLCLQQSEPQYYTDDIIKAHVLTMLV 314
           RAD FLQ LID ERD+R SRWPE K GN TMIQSLL LQ+S+PQ+Y+DDIIK HVLTML 
Sbjct: 260 RADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDDIIKGHVLTMLA 319

Query: 315 AGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKLQYLQAIIYETF 374
           AGTDTTTGTL WAMSLLLNHP IMNKAW+EI                 L+YL AIIYET 
Sbjct: 320 AGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLKYLVAIIYETL 379

Query: 375 RLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFVDWE 434
           RL+P APLLVPHE+SE+C +EGFD+PKGTMLLVNAWA+HRDP+VWEDPT FRPERF++WE
Sbjct: 380 RLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTSFRPERFLNWE 439

Query: 435 GNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNGITM 492
                S+KW+PFG+GRRACPGA L  RVVGLTLATLIQCFEW+R+GEE +DLS+G GIT 
Sbjct: 440 --TAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSQGTGITT 499

BLAST of Sed0013457 vs. NCBI nr
Match: KAG7024282.1 (Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 688.0 bits (1774), Expect = 6.1e-194
Identity = 348/515 (67.57%), Postives = 402/515 (78.06%), Query Frame = 0

Query: 3   EIEKLSWLTHFLISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQ 62
           E   ++WL     S +LLF L LI K RK   +  PPSPP LP+IGHLHLLK PFHRVLQ
Sbjct: 2   EANTITWL-----SLSLLFTLSLIYKLRKQSPRRCPPSPPSLPLIGHLHLLKQPFHRVLQ 61

Query: 63  NLSQKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAF 122
           +LS K GPI  LSIGSR +VVISSP A ++CFTKNDI  ANRPRLL+G Y+NYNYTGVA 
Sbjct: 62  DLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIAFANRPRLLSGNYMNYNYTGVAV 121

Query: 123 AAYGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV------- 182
           A+YGHHWRNMRR+AT ELLSN RL   L++R  ELKL +K+LY  TGE+  FV       
Sbjct: 122 ASYGHHWRNMRRVATKELLSNQRLDTYLNIRVQELKLCVKNLYGATGESSDFVRLEMQSK 181

Query: 183 ----SFNTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGF 242
               SFNTVMRMVSGKRYFG  VE+ EE+REFR IMKE+++LSGASNP DFLPILR+  +
Sbjct: 182 LNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLSGASNPADFLPILRLFDY 241

Query: 243 GGLEKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGN-TMIQSLLCLQQSEPQYYTD 302
            GLEKRM++AS RAD FLQ LID ER++R SRWPE K GN TMIQSLL  Q+S+PQYY+D
Sbjct: 242 QGLEKRMVKASSRADLFLQNLIDCERERRSSRWPEEKHGNKTMIQSLLSFQESQPQYYSD 301

Query: 303 DIIKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YK 362
           DIIK HVLTML AGTDTTTGT+ WAMSLLLNHPTIMNKAW+EI                 
Sbjct: 302 DIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMNKAWSEIRECVGESRLVEEGDVSS 361

Query: 363 LQYLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDP 422
           L+Y++AIIYET RL+P+APLLVPHE+SE+C IEGFD+PKGTMLLVNAWA+HRDP++WEDP
Sbjct: 362 LKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWEDP 421

Query: 423 TRFRPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEE 482
           T FRPERF++WE     S+KW+ FG+GR ACPGA L  RVVGLTLATLIQCFEW+R+GEE
Sbjct: 422 TSFRPERFLNWEA--AESHKWVLFGVGRGACPGAALAHRVVGLTLATLIQCFEWQRVGEE 481

Query: 483 EVDLSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
            +DLSEG GITMPK  PL AM R RSSM+HLLS L
Sbjct: 482 PIDLSEGTGITMPKAIPLVAMCRARSSMLHLLSPL 509

BLAST of Sed0013457 vs. NCBI nr
Match: XP_038896686.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 684.1 bits (1764), Expect = 8.9e-193
Identity = 341/514 (66.34%), Postives = 398/514 (77.43%), Query Frame = 0

Query: 3   EIEKLSWLTHFLISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQ 62
           E   ++W   FLIS   LF  +L+SK RK ++K LPPSPP LPVIGHLHLL++P HRVL 
Sbjct: 2   EPHTITWFFPFLIS-LFLFTFILLSKLRKINHKKLPPSPPSLPVIGHLHLLRDPLHRVLH 61

Query: 63  NLSQKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAF 122
           NLS K GPI  LSIGSR VVV+SSP A ++CFTKNDIV ANRPRLL+GKYINYNYT V F
Sbjct: 62  NLSNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGF 121

Query: 123 AAYGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV------- 182
           A YG HWRNMRRIATTELLSNHRL   L++R  ELKLW+K+LY        FV       
Sbjct: 122 APYGQHWRNMRRIATTELLSNHRLNTYLNIRVQELKLWIKNLYKLICREDNFVGVEMKSK 181

Query: 183 ----SFNTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGF 242
               SFN VMRMVSGKRYFG  V + EE+ EFR + KE++ELSGA+NP DFL ILR+  F
Sbjct: 182 LKELSFNIVMRMVSGKRYFGVEVVDVEEALEFRELTKELLELSGATNPADFLTILRVFDF 241

Query: 243 GGLEKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDD 302
            GL KRM++AS RAD FLQKLIDE+R++R+SRWPE K G +MIQ+LL LQ+S+P YY+DD
Sbjct: 242 QGLNKRMLKASGRADVFLQKLIDEQRERRLSRWPEDKHGKSMIQTLLSLQESQPDYYSDD 301

Query: 303 IIKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKL 362
           IIK HVLTML AGTDTT+GT+ W MSLLLNHPT+M KAW EI                 L
Sbjct: 302 IIKGHVLTMLAAGTDTTSGTIEWTMSLLLNHPTVMKKAWIEIRDCVGENRMVEEADVSNL 361

Query: 363 QYLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPT 422
           +Y++AIIYET RL+P APLLVPHE+SEDC IEGF+VPKGTMLLVNAWA+HRDPKVWEDP 
Sbjct: 362 RYMEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEVPKGTMLLVNAWAIHRDPKVWEDPN 421

Query: 423 RFRPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEE 482
            FRPERF++WEG    SYKW+PFGMGRRACPGA L QRVVGLTLATLIQCFEW+++ E++
Sbjct: 422 SFRPERFLNWEG--VESYKWIPFGMGRRACPGAALAQRVVGLTLATLIQCFEWEKVDEQQ 481

Query: 483 VDLSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
           +DLSEG+GITMPK   L AM + R+SM+HLLS L
Sbjct: 482 IDLSEGSGITMPKAKALVAMCKPRNSMLHLLSCL 512

BLAST of Sed0013457 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 5.5e-137
Identity = 260/505 (51.49%), Postives = 341/505 (67.52%), Query Frame = 0

Query: 14  LISTTLLFILVLISKFRKP---HYKSLPPSPPY-LPVIGHLHLLKNPFHRVLQNLSQKLG 73
           L+ T L  +L+ IS    P     ++LPPSP   LPVIGHLHL+    HR L +LS+K G
Sbjct: 6   LLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYG 65

Query: 74  PIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHHW 133
            +F L +G+R V+V+SSPAAA++CFTKNDIV ANRP  + GKYI YNYT +  + YG HW
Sbjct: 66  SVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHW 125

Query: 134 RNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV---------SFNTV 193
           RN+RR+A  E+ S   L   LS+R  E+K  L  LY  +G++ G V         SFN  
Sbjct: 126 RNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVT 185

Query: 194 MRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKRMME 253
           MRMV+GKRYFG  V++ +E++ FR ++ E+ E +GASNP DF+P LR I F   EK++ +
Sbjct: 186 MRMVAGKRYFGQDVDS-DEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVSK 245

Query: 254 ASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDIIKAHVLTM 313
            S+  D FLQ+LI E R          K+  TMI  LL LQ+S+P+YYTD IIK  ++ +
Sbjct: 246 ISQEMDAFLQRLIHESR--------INKNNVTMIDHLLSLQESQPEYYTDQIIKGIIMVL 305

Query: 314 LVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEISY--------------KLQYLQAIIYE 373
           L+AGTDT+  T+ WAMSLLLNHP  + KA TEI                KL YL  II E
Sbjct: 306 LLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISE 365

Query: 374 TFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFVD 433
           TFRL P AP+LVPHE+S+DC ++G+DVPKGT+LLVNAWA+HRDP+ W++PT F+PER   
Sbjct: 366 TFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPER--- 425

Query: 434 WEGNIE-SSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNG 491
             G +E    K MPFGMGRR+CPG+ L QRVVGLTL  LIQCFEWKRIGE ++D++EG+G
Sbjct: 426 -HGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSG 485

BLAST of Sed0013457 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 4.1e-132
Identity = 259/501 (51.70%), Postives = 329/501 (65.67%), Query Frame = 0

Query: 10  LTHFLIS--TTLLFILVLISKF--RKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQNLS 69
           +T F +S   +L F+++ +  F      +K+LPP P  LP+IG+LH LK P H     LS
Sbjct: 1   MTTFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLS 60

Query: 70  QKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAY 129
           QK G IF L  GSR VVV+SS   AQ+CFTKNDIVLANRP  L GKYI YN T VA + Y
Sbjct: 61  QKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPY 120

Query: 130 GHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDL----YN-----ETGENRGFVS 189
           G HWRN+RRI + E+LS+HRL + L +R  E+   ++ L    YN     E       ++
Sbjct: 121 GDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMT 180

Query: 190 FNTVMRMVSGKRYFG--AGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGL 249
           FNT+MRMVSGKRY+G    V + EE+R FRGI+KE+V L GA+N  DFL  LR   F GL
Sbjct: 181 FNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGL 240

Query: 250 EKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDIIK 309
           EKR+ + S+R D FLQ LIDE R  +       ++ NTMI  LL  QQS+P+YYTD IIK
Sbjct: 241 EKRLKKISKRTDAFLQGLIDEHRFGK-------RNSNTMIDHLLTQQQSQPEYYTDQIIK 300

Query: 310 AHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKLQYL 369
             ++ ML+AGTDT++ T+ WAMS LLNHP IM KA  E+                KL YL
Sbjct: 301 GLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYL 360

Query: 370 QAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFR 429
           Q+I+YET RL+  APLLVPH +SED ++ G+++P+ T+L+VNAW +HRDP +W DPT F+
Sbjct: 361 QSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFK 420

Query: 430 PERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDL 482
           PERF + EG +    K + FG+GRRACPG  L QR  GLTL  LIQCFEWKRIGEE++D+
Sbjct: 421 PERF-EKEGEVN---KLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDM 480

BLAST of Sed0013457 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 4.4e-126
Identity = 241/492 (48.98%), Postives = 314/492 (63.82%), Query Frame = 0

Query: 14  LISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQNLSQKLGPIFF 73
           L+S + +  + ++ K      K+LPP PP +P+IG+LH LK+P HR    LSQ  G IF 
Sbjct: 7   LLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFS 66

Query: 74  LSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHHWRNMR 133
           L  GSR VVV+SSP+ A +CFTKNDI+LANRPR L GKYI YNYT +  A+YG HWRN+R
Sbjct: 67  LWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLR 126

Query: 134 RIATTELLSNHRLGNDLSVRAHEL-KLWLKDLYNETGENRGF-----------VSFNTVM 193
           RI T ++LSN+RL + L VR  E  +L  K L +   E  GF           ++FN +M
Sbjct: 127 RITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMM 186

Query: 194 RMVSGKRYFG--AGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKRMM 253
           RM+SGKRY+G    V + EE+++FR I+ E++ L GA+N  DFLP+LR++    LEKR  
Sbjct: 187 RMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCK 246

Query: 254 EASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDIIKAHVLT 313
             ++R++ FL+ LI+E R   +       DG TMI  LL L +S+P+YY+D +IK  +  
Sbjct: 247 RIAKRSNAFLEGLIEEHRRGNIH-----SDGGTMIDHLLKLSESQPEYYSDHLIKGLIQG 306

Query: 314 MLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKLQYLQAIIY 373
           ML+AGTDT+  T+ W MS LLNHP ++ KA  E+                KL YLQ II 
Sbjct: 307 MLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIIS 366

Query: 374 ETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFV 433
           ET RL+P APLL+PH +SEDCTI  F+VPK T++L N W +HRDPK W D   F+PERF 
Sbjct: 367 ETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERF- 426

Query: 434 DWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNG 478
                 E   K M FG+GRRACPG  L QR VG T+  LIQCFEW+R  EE++D+ EG G
Sbjct: 427 ---EKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKG 486

BLAST of Sed0013457 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 449.5 bits (1155), Expect = 4.8e-125
Identity = 233/503 (46.32%), Postives = 328/503 (65.21%), Query Frame = 0

Query: 4   IEKLSWLTHFLISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQN 63
           +E LS L++ +    L FI  ++ + RK  +K+LPP PP LP+IG+LH LK P HR  + 
Sbjct: 1   MEILSLLSYSVFYLALFFIFNIVIRARK--FKNLPPGPPSLPIIGNLHHLKRPLHRTFKG 60

Query: 64  LSQKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFA 123
           LS+K G +F L  GSR VVV+SS +  QQCFTKND+VLANRPR L+GKYI YNYT +   
Sbjct: 61  LSEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGST 120

Query: 124 AYGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRG---------F 183
           +YG HWRN+RRI   ++LSNHR+ +   +R  E +  +  L +++  N            
Sbjct: 121 SYGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYD 180

Query: 184 VSFNTVMRMVSGKRYFGAGVENG--EESREFRGIMKEIVELSGASNPVDFLPILRMIGFG 243
           ++FN +MRM+SGKRY+G   +    +E+ +FR ++ E+++LSGA+N  DF+P+LR + F 
Sbjct: 181 MTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFE 240

Query: 244 GLEKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDI 303
            LEKR+ + S + D FL+ LI+E R K+       +  NTMI  LL LQ S+P+YYTD I
Sbjct: 241 NLEKRLKDISGKTDAFLRGLIEEHRTKK-------ERANTMIDHLLNLQDSQPEYYTDQI 300

Query: 304 IKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEISY--------------KLQ 363
           IK   L ML+AGTD++  TL W+MS LLNHP ++ K   E+                KL 
Sbjct: 301 IKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLT 360

Query: 364 YLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTR 423
           YL+ +I ET RLY  APLL+PH  S++C I G+ VP+ T++L+NAWA+HRDP++W + T 
Sbjct: 361 YLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATT 420

Query: 424 FRPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEV 482
           F+PERF + +G +E   K + FGMGRRACPG  L  R + +TLA LIQCF+WK I  +++
Sbjct: 421 FKPERF-EKKGELE---KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKI 480

BLAST of Sed0013457 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 2.6e-123
Identity = 228/500 (45.60%), Postives = 328/500 (65.60%), Query Frame = 0

Query: 7   LSWLTHFLISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQNLSQ 66
           LS+L + L   ++ FI+ L+ + RK  +K+LPP P  LP+IG+LH LK P +R  + L++
Sbjct: 4   LSYLCYSLFYLSIFFIIRLLFQSRK--FKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTE 63

Query: 67  KLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYG 126
           K G +  L  GSR VVV+SS +  Q+CFTKND+VLANRPR L+GKYI YNYT +   +YG
Sbjct: 64  KYGNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYG 123

Query: 127 HHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGEN--------RGF-VSF 186
            HWRN+RRI + ++LSNHR+ N   +R  E +  +K L  ++           R F ++F
Sbjct: 124 EHWRNLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTF 183

Query: 187 NTVMRMVSGKRYFG--AGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLE 246
           N +MRM+SGKRY+G    +   +E+ +FR ++ E+++LSGA+N  DF+P+L+ + F  LE
Sbjct: 184 NNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLE 243

Query: 247 KRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDIIKA 306
           KR+    E+ D FL  L+ E+R K+       +  NTMI  LL +Q+S+P+YYTD IIK 
Sbjct: 244 KRVKRIGEKNDVFLSGLLQEQRSKK-------ERTNTMIDHLLNMQESQPEYYTDTIIKG 303

Query: 307 HVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEISY--------------KLQYLQ 366
             L ML+AGTD++  TL W MS +LN+P ++ K   E+                KL YL+
Sbjct: 304 LCLAMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLR 363

Query: 367 AIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRP 426
            +IYET RLY  APLL+PH  +++C + G+ VP+ T++L+NAWA+HRDP+ W + T F+P
Sbjct: 364 NVIYETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKP 423

Query: 427 ERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLS 482
           ERF D +G +E   K + FGMGRRACPG  L  R + +TLA L+QCF+WKRI +E++D+S
Sbjct: 424 ERF-DKKGELE---KMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMS 483

BLAST of Sed0013457 vs. ExPASy TrEMBL
Match: A0A6J1F8L9 (isoflavone 3'-hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443310 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 2.4e-199
Identity = 354/515 (68.74%), Postives = 407/515 (79.03%), Query Frame = 0

Query: 3   EIEKLSWLTHFLISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQ 62
           E   ++WL     S +LLF L LI K RK   +  PPSPP LP+IGHLHLLK PFHRVLQ
Sbjct: 2   EANTITWL-----SLSLLFTLSLIYKLRKQSPRRCPPSPPSLPLIGHLHLLKQPFHRVLQ 61

Query: 63  NLSQKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAF 122
           +LS K GPI  LSIGSR +VVISSP A ++CFTKNDI  ANRPRLL+GKY+NYNYTGVA 
Sbjct: 62  DLSDKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGVAV 121

Query: 123 AAYGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV------- 182
           A+YGHHWRNMRRIATTELLSNHRL   L++R  ELKLW+K+LY ETGE+  FV       
Sbjct: 122 ASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWVKNLYGETGESSDFVRLEMQSK 181

Query: 183 ----SFNTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGF 242
               SFNTVMRMVSGKRYFG  VE+ EE+REFR IMKE++ LSGASNP DFLPILR+  +
Sbjct: 182 LNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLHLSGASNPADFLPILRLFDY 241

Query: 243 GGLEKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGN-TMIQSLLCLQQSEPQYYTD 302
            GLEKRM++AS RAD FLQ LID ER++R SRWPE K GN TMIQSLL  Q+S+PQYY+D
Sbjct: 242 QGLEKRMVKASGRADLFLQNLIDCERERRASRWPEEKHGNKTMIQSLLSFQESQPQYYSD 301

Query: 303 DIIKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YK 362
           DIIK HVLTML AGTDTTTGT+ WAMSLLLNHPTIM+KAW+EI                 
Sbjct: 302 DIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMDKAWSEIRECVGESRLVEEGDVSS 361

Query: 363 LQYLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDP 422
           L+Y++AIIYET RL+P+APLLVPHE+SE+C IEGFD+PKGTMLLVNAWA+HRDP++WEDP
Sbjct: 362 LKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWEDP 421

Query: 423 TRFRPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEE 482
           T FRPERF++WE     S+KW+PFG+GRRACPG  L  RVVGLTLATLIQCFEW+R+GEE
Sbjct: 422 TSFRPERFLNWEA--AESHKWVPFGVGRRACPGVALAHRVVGLTLATLIQCFEWQRVGEE 481

Query: 483 EVDLSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
            +DLSEG GITMPK  PL AM R RSSM+HLLS L
Sbjct: 482 PIDLSEGTGITMPKAIPLVAMCRARSSMLHLLSPL 509

BLAST of Sed0013457 vs. ExPASy TrEMBL
Match: A0A6J1IL26 (isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477203 PE=3 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 2.2e-197
Identity = 352/503 (69.98%), Postives = 402/503 (79.92%), Query Frame = 0

Query: 15  ISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQNLSQKLGPIFFL 74
           +S +LL  L+LI K RK   ++ PPSPP LP+IGHLHLLK PFHRVLQ+LS K GPI  L
Sbjct: 20  LSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQDLSNKHGPILSL 79

Query: 75  SIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHHWRNMRR 134
           SIGSR +VVISSP A ++CFTKNDI LANRPRLLAGKY+NYNYTGVAFA+YGHHWRNMRR
Sbjct: 80  SIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFASYGHHWRNMRR 139

Query: 135 IATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV-----------SFNTVMRM 194
           IATTELLSNHRL   L++R  ELKL +K+LY  TGE+  FV           SFN VMRM
Sbjct: 140 IATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFVRLEMQSKLNELSFNIVMRM 199

Query: 195 VSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKRMMEASE 254
           VSGKRYFG  VE+ EE+REFR IMKE+++L GASNP DFLPILR+  + GLEKRM++AS 
Sbjct: 200 VSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQGLEKRMVQASG 259

Query: 255 RADGFLQKLIDEERDKRVSRWPEGKDGN-TMIQSLLCLQQSEPQYYTDDIIKAHVLTMLV 314
           RAD FLQ LID ERD+R SRWPE K GN TMIQSLL LQ+S+PQ+Y+DDIIK HVLTML 
Sbjct: 260 RADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDDIIKGHVLTMLA 319

Query: 315 AGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKLQYLQAIIYETF 374
           AGTDTTTGTL WAMSLLLNHP IMNKAW+EI                 L+YL AIIYET 
Sbjct: 320 AGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLKYLVAIIYETL 379

Query: 375 RLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFVDWE 434
           RL+P APLLVPHE+SE+C +EGFD+PKGTMLLVNAWA+HRDP+VWEDPT FRPERF++WE
Sbjct: 380 RLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTSFRPERFLNWE 439

Query: 435 GNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNGITM 492
                S+KW+PFG+GRRACPGA L  RVVGLTLATLIQCFEW+R+GEE +DLS+G GIT 
Sbjct: 440 --TAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSQGTGITT 499

BLAST of Sed0013457 vs. ExPASy TrEMBL
Match: A0A5A7VB12 (Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002950 PE=3 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 2.3e-186
Identity = 335/512 (65.43%), Postives = 396/512 (77.34%), Query Frame = 0

Query: 10  LTHFLISTTLLFILVLISKFRK-PHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQNLSQKL 69
           +T F +S  L+  L+L+SK RK  + K LPP+PP LP+IGHLHLLK PFHRVL NLS K 
Sbjct: 14  ITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHNLSNKY 73

Query: 70  GPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHH 129
           GPI  L+IGSR V+V+SSP A ++CFTKNDIV ANRPRL++GKYINYNYT V FA YG H
Sbjct: 74  GPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFAPYGQH 133

Query: 130 WRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV-----------SF 189
           WRNMRRIATTELLSNHRL   L++R  ELKLW+K+   + G    FV           SF
Sbjct: 134 WRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKLKELSF 193

Query: 190 NTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKR 249
           NTVMRM+SGKRYFG  VE+ EE+ EFR IMKE++ELSGA+N  DFL ILR+    G++KR
Sbjct: 194 NTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLEGVKKR 253

Query: 250 MMEASERADGFLQKLIDEERDKRVSRWPEGKD---GNTMIQSLLCLQQSEPQYYTDDIIK 309
           MM+AS RAD FLQKLIDEER KRVSRWPE K      +MI++LL LQ+S+PQYY+DDIIK
Sbjct: 254 MMKASGRADVFLQKLIDEERKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYYSDDIIK 313

Query: 310 AHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKLQYL 369
            HVLTML AGTDTT+GT+ WAMSLLLNHP  M KAW EI                +L+YL
Sbjct: 314 GHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVSELKYL 373

Query: 370 QAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFR 429
           +AIIYET R++P APLLVPHE SEDC+IEGF+VPKGTMLLVNAW +HRDPKVWEDPT F+
Sbjct: 374 EAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWEDPTSFQ 433

Query: 430 PERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGE-EEVD 489
           PERF++WEG    +YKW+PFGMGRRACPG  L QR +GLTLATLIQCFEWK++ E E++D
Sbjct: 434 PERFLNWEG--VETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQEQID 493

Query: 490 LSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
           LSEG+GITMPK   LEAM + R+SM+HLL+QL
Sbjct: 494 LSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 523

BLAST of Sed0013457 vs. ExPASy TrEMBL
Match: A0A1S3BWG9 (LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493891 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.8e-183
Identity = 332/512 (64.84%), Postives = 394/512 (76.95%), Query Frame = 0

Query: 10  LTHFLISTTLLFILVLISKFRK-PHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQNLSQKL 69
           +T F +S  L+  L+L+SK RK  + K LPP+PP LP+IGHLHLLK PFHRVL NLS K 
Sbjct: 11  ITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHNLSNKY 70

Query: 70  GPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHH 129
           GPI  L+IGSR V+V+SSP A ++CFTKNDIV ANRPRL++GKYINYNYT V FA YG H
Sbjct: 71  GPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFAPYGQH 130

Query: 130 WRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV-----------SF 189
           WRNMRRIATTELLSNHRL   L++R  ELKLW+K+   + G    FV           SF
Sbjct: 131 WRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKLKELSF 190

Query: 190 NTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKR 249
           NTVMRM+SGKRYFG  VE+ EE+ EFR IMKE++ELSGA+N  DFL ILR+    G++KR
Sbjct: 191 NTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLEGVKKR 250

Query: 250 MMEASERADGFLQKLIDEERDKRVSRWPEGKD---GNTMIQSLLCLQQSEPQYYTDDIIK 309
           MM+AS RAD FLQKLIDEER K+  RWPE K      +MI++LL LQ+S+PQYY+DDIIK
Sbjct: 251 MMKASGRADVFLQKLIDEER-KKEFRWPEEKQKQAKTSMIRTLLSLQESQPQYYSDDIIK 310

Query: 310 AHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKLQYL 369
            HVLTML AGTDTT+GT+ WAMSLLLNHP  M KAW EI                +L+YL
Sbjct: 311 GHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVSELKYL 370

Query: 370 QAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFR 429
           +AIIYET R++P APLLVPHE SEDC+IEGF+VPKGTMLLVNAW +HRDPKVWEDPT F+
Sbjct: 371 EAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWEDPTSFQ 430

Query: 430 PERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGE-EEVD 489
           PERF++WEG    +YKW+PFGMGRRACPG  L QR +GLTLATLIQCFEWK++ E E++D
Sbjct: 431 PERFLNWEG--VETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQEQID 490

Query: 490 LSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
           LSEG+GITMPK   LEAM + R+SM+HLL+QL
Sbjct: 491 LSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 519

BLAST of Sed0013457 vs. ExPASy TrEMBL
Match: A0A6J1C4W6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111008503 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.8e-162
Identity = 297/514 (57.78%), Postives = 369/514 (71.79%), Query Frame = 0

Query: 4   IEKLSWLTHFLISTTLLFILVLI--SKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVL 63
           +E++   + FL+   LLF L+ +   KF+K   K+LPP PP LPVIGHLHLL+ PFH++L
Sbjct: 1   MEEILQFSRFLVPLALLFALIHVFNFKFKKVSSKNLPPXPPSLPVIGHLHLLREPFHQML 60

Query: 64  QNLSQKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVA 123
           QNLSQ+ GPI  LS+G RSVVV+SSP AAQ+CFTKNDIV ANRPR+++GK +NYNYT V 
Sbjct: 61  QNLSQRYGPILSLSLGFRSVVVVSSPIAAQECFTKNDIVFANRPRMISGKILNYNYTTVG 120

Query: 124 FAAYGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV------ 183
             AYG HWR MRRIATTELLS HRL + +++R  E+KLWLK+LY  TG+N   V      
Sbjct: 121 ATAYGQHWRIMRRIATTELLSTHRLISFINLRQEEVKLWLKNLYRITGQNNVRVEIRSRL 180

Query: 184 ---SFNTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFG 243
              SFN V+RM++GKRYFG  V++ EE RE + +MKE+  LSG S P DFL +LR+I + 
Sbjct: 181 TELSFNIVLRMMTGKRYFGVEVKDSEEERELKKVMKEVSLLSGMSYPADFLSVLRLIDYQ 240

Query: 244 GLEKRMMEASERADGFLQKLIDEERDKRVS-RWPEGKDGNTMIQSLLCLQQSEPQYYTDD 303
           G  KR+ +    ADGF+Q LID  R+ + S  +   ++   MI ++L LQ S+P YY D 
Sbjct: 241 GFRKRLWKVWAGADGFVQXLIDGRREMKASCSFSLEENWKNMIDTMLSLQDSDPDYYKDH 300

Query: 304 IIKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKL 363
           IIK  +LT+LVAGTDTT GT+ W MSLLLNHPTIM KAW+EI+                L
Sbjct: 301 IIKGQILTLLVAGTDTTAGTIEWGMSLLLNHPTIMKKAWSEINDYVGEQRLVEEADLANL 360

Query: 364 QYLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPT 423
            YLQAII ET+RL+P  P+LVP E+SEDCTI G+DVPKGTML+VN WA+HRDP+VW+DPT
Sbjct: 361 PYLQAIINETYRLFPALPILVPRESSEDCTIGGYDVPKGTMLVVNMWAIHRDPQVWQDPT 420

Query: 424 RFRPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEE 483
           RF+PERF +  G +    K +PFGMGRRACPGA L  RVVGLTLATLIQCFEW RI   E
Sbjct: 421 RFQPERFEN--GEVVEVNKLLPFGMGRRACPGAGLAHRVVGLTLATLIQCFEWDRIDPNE 480

Query: 484 VDLSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
           +DL +GNG+TMPK  PLEAM + R  M+ LLS L
Sbjct: 481 IDLXQGNGLTMPKAHPLEAMCKPRGPMISLLSHL 512

BLAST of Sed0013457 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 461.1 bits (1185), Expect = 1.1e-129
Identity = 244/505 (48.32%), Postives = 338/505 (66.93%), Query Frame = 0

Query: 13  FLISTTLLFILVLISKF-RKPHYKSLPPSPPY-LPVIGHLHLLKNPFHRVLQNLSQKL-- 72
           F I   +L ++ LI K  RKP   +LPPSP + LPVIGHL LLK P HR   +LSQ L  
Sbjct: 8   FSILFVVLSLIYLIGKLKRKP---NLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNN 67

Query: 73  GPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHH 132
            PIF L +G+R V V SS + A++CFTKND+VLANRP  +  K++ Y+YT +  A+YG H
Sbjct: 68  APIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDH 127

Query: 133 WRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGEN---------RGFVSFNT 192
           WRN+RRI + E+ SNHRL + LS+R  E++  +  L     +             ++FN 
Sbjct: 128 WRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNN 187

Query: 193 VMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKRMM 252
           ++RMV+GKRY+G GVE+  E++  R ++ ++V  +GA N VD+LP+LR++     E R+ 
Sbjct: 188 ILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVK 247

Query: 253 EASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDIIKAHVLT 312
           + + R D FLQ L+DE+R+ +       + GNTMI  LL LQ+S+P Y+TD IIK ++L 
Sbjct: 248 KLAGRLDEFLQGLVDEKREAK-------EKGNTMIDHLLTLQESQPDYFTDRIIKGNMLA 307

Query: 313 MLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEISYK--------------LQYLQAIIY 372
           +++AGTDT+  TL WA+S +LNHP ++NKA  EI  K              L YLQ I+ 
Sbjct: 308 LILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVS 367

Query: 373 ETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFV 432
           ET RLYP AP+L+PH ASEDC + G+D+P+GT+LL N WA+HRDP++W+DP  F+PERF 
Sbjct: 368 ETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERF- 427

Query: 433 DWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNG 491
           + EG  +   K MPFG+GRRACPG+ L  R++ LTL +LIQC EW++IG EEVD+SEG G
Sbjct: 428 EKEGEAQ---KLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIG-EEVDMSEGKG 487

BLAST of Sed0013457 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 444.1 bits (1141), Expect = 1.4e-124
Identity = 239/503 (47.51%), Postives = 321/503 (63.82%), Query Frame = 0

Query: 14  LISTTLLFILVLISKFRKPHYKSLPPSPPY-LPVIGHLHLLKNPFHRVLQNLSQKLG--P 73
           ++S   LF L +    R     +LPPSP   LPVIGHLHLLK P HR   +LS+ +G  P
Sbjct: 8   ILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAP 67

Query: 74  IFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHHWR 133
           +F L +G+R V VISS + A++CFTKND+VLANRP+    K++ YN T +  A+YG HWR
Sbjct: 68  VFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWR 127

Query: 134 NMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV-----------SFNT 193
           N+RRIA  E+ S HRL + L +R  E++  +  L  ++    GFV           + NT
Sbjct: 128 NLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDS--LHGFVEVEMKTLLTNLASNT 187

Query: 194 VMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKRMM 253
            +RM++GKRYFG   E+ ++++  + ++ E V  +GA NP+D+L ILR +     EKR+ 
Sbjct: 188 TIRMLAGKRYFG---EDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKRIK 247

Query: 254 EASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDIIKAHVLT 313
               R D FLQKL+DE+R        E + G TMI  LL LQ  +P YYTD IIK  +LT
Sbjct: 248 NLGNRFDTFLQKLVDEKR-------AEKEKGETMIDHLLALQDIQPDYYTDVIIKGIILT 307

Query: 314 MLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEISYK--------------LQYLQAIIY 373
           +++AGTDT++ TL WAMS LLNHP I+ KA  EI  K              L YLQ+I+ 
Sbjct: 308 LIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVL 367

Query: 374 ETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFV 433
           ET R+YP  PLL+PH +SEDC + G+D+P GTM+L NAWAMHRDP+VWEDP  F+PERF 
Sbjct: 368 ETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERF- 427

Query: 434 DWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNG 489
           + EG  E   K + FGMGRRACPGA L  R++   L +L+QCFEW+R+GE+ VD++E  G
Sbjct: 428 EKEGEAE---KLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKG 487

BLAST of Sed0013457 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 443.4 bits (1139), Expect = 2.5e-124
Identity = 236/493 (47.87%), Postives = 320/493 (64.91%), Query Frame = 0

Query: 18  TLLFILVLISKFRKPHYKSLPPSPPY-LPVIGHLHLLKNPFHRVLQNLSQKLG--PIFFL 77
           T+   L+L    RKP   +LPPSP Y LPVIGHLHLLK P HR   ++S+ LG  PIF L
Sbjct: 15  TISIKLLLTKSNRKP---NLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPIFHL 74

Query: 78  SIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHHWRNMRR 137
            +G+R V VISS + A++CFTKND+VLANRP ++  K++ YN+T +  A+YG HWRN+RR
Sbjct: 75  RLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRNLRR 134

Query: 138 IATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV-----------SFNTVMRM 197
           IA  E+ S+HR+    S+R  E++  +  L  ++    GFV           +FN ++ M
Sbjct: 135 IAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDS--LHGFVEVELKSLLTNLAFNNIIMM 194

Query: 198 VSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKRMMEASE 257
           V+GKRY+G G E+ +E++  R ++ EI+  +G+ N  D+LP +  +     E +      
Sbjct: 195 VAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFENQTKILGN 254

Query: 258 RADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDIIKAHVLTMLVA 317
           R D  LQKL+DE+R        E + G T+I  LL  Q++EP+YYTD IIK  +L +++A
Sbjct: 255 RLDRVLQKLVDEKR-------AEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLA 314

Query: 318 GTDTTTGTLVWAMSLLLNHPTIMNKAWTEISYK--------------LQYLQAIIYETFR 377
           GTDT++ TL WAMS LLNHP I+ KA  EI  K              L YLQ I+ ET R
Sbjct: 315 GTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLR 374

Query: 378 LYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFVDWEG 437
           LYP  PLL+PH +S++C + G+D+P+ T+LL N WAMHRDP +WE+P RF+PERF + EG
Sbjct: 375 LYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERF-EKEG 434

Query: 438 NIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNGITMP 483
               + K MPFGMGRRACPGA LG+R+V L L  LIQ FEW+R+G E VD++EG GITMP
Sbjct: 435 ---EARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMP 489

BLAST of Sed0013457 vs. TAIR 10
Match: AT4G37400.1 (cytochrome P450, family 81, subfamily F, polypeptide 3 )

HSP 1 Score: 441.4 bits (1134), Expect = 9.3e-124
Identity = 234/481 (48.65%), Postives = 314/481 (65.28%), Query Frame = 0

Query: 36  SLPPSPPY-LPVIGHLHLLKNPFHRVLQNLSQKLGPIFFLSIGSRSVVVISSPAAAQQCF 95
           +LPPSPPY LP++GH +LLK P HR+   LS+  GPIF L  GSR  VVISS + A QCF
Sbjct: 29  NLPPSPPYSLPILGHHNLLKPPVHRLFHRLSKTHGPIFSLQFGSRRAVVISSSSLATQCF 88

Query: 96  T-KNDIVLANRPRLLAGKYINYNYTGVAFAAYGHHWRNMRRIATTELLSNHRLGNDLSVR 155
           T +NDI+L+NRP  L  KY+ YNYT V  A YG HWRN+RRI + E+LS++RL N L +R
Sbjct: 89  TGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGDHWRNLRRICSLEILSSNRLTNFLHIR 148

Query: 156 AHELKLWLKDLYNETGEN------RGFVSFNTVMRMVSGKRYFGAGVENGEESREFRGIM 215
             E+   L  L  +  +          ++FN ++RMV+GKRY+G  V N EE+  F+ ++
Sbjct: 149 KDEIHRMLTRLSRDVNKEIELEPLLSDLTFNNIVRMVTGKRYYGDEVHNEEEANVFKKLV 208

Query: 216 KEIVELSGASNPVDFLPILRMIGFGGLEKRMMEASERADGFLQKLIDE-ERDKRVSRWPE 275
            +I + SGA +P D+LP ++M G G  EK++   +E  D  LQ+L++E +RD        
Sbjct: 209 ADINDCSGARHPGDYLPFMKMFG-GSFEKKVKALAEAMDEILQRLLEECKRD-------- 268

Query: 276 GKDGNTMIQSLLCLQQSEPQYYTDDIIKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIM 335
            KDGNTM+  LL LQQ+EP+YYTD  IK  +L M++AGTDT+  TL WAMS LLNHP  +
Sbjct: 269 -KDGNTMVNHLLSLQQNEPEYYTDVTIKGLMLGMMIAGTDTSAVTLEWAMSSLLNHPEAL 328

Query: 336 NKAWTEISYK--------------LQYLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFD 395
            KA  EI  K              L YLQ I+ ETFRLYP APLLVP   +ED  + G+D
Sbjct: 329 EKAKLEIDEKIGQERLIDEPDIANLPYLQNIVSETFRLYPAAPLLVPRSPTEDIKVGGYD 388

Query: 396 VPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFVDWE--GNIESSYKWMPFGMGRRACPGA 455
           VP+GTM++VNAWA+HRDP++W +P +F+PERF   E  G  E  +K MPFG GRR+CPGA
Sbjct: 389 VPRGTMVMVNAWAIHRDPELWNEPEKFKPERFNGGEGGGRGEDVHKLMPFGNGRRSCPGA 448

Query: 456 VLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNGITMPKTTPLEAMFRVRSSMVHLLSQ 492
            LGQ++V L L +LIQCF+W+++  E +D++E  G+ M K  PL A+ + R  M  L + 
Sbjct: 449 GLGQKIVTLALGSLIQCFDWQKVNGEAIDMTETPGMAMRKKIPLSALCQSRPIMSKLQAH 499

BLAST of Sed0013457 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 440.7 bits (1132), Expect = 1.6e-123
Identity = 240/503 (47.71%), Postives = 325/503 (64.61%), Query Frame = 0

Query: 11  THFLISTTLLFILVLISKF---RKPHYKSLPPSPPY-LPVIGHLHLLKNPFHRVLQNLSQ 70
           T +LI +   F + L  K     +P   +LPPSP    P+IGHLHLLK P HR   +LS+
Sbjct: 10  TIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSE 69

Query: 71  KL--GPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAA 130
            L    IF LS+GSR V V+SS A A++CFTKND+VLANRP  L GK+I YN T +  AA
Sbjct: 70  SLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAA 129

Query: 131 YGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV--------- 190
           YG  WRN+RRI T E+ S+ RL + +S+R  E++  +  L   +    GFV         
Sbjct: 130 YGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNS--QHGFVKVEMKPLFM 189

Query: 191 --SFNTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGG 250
             + N ++RMV+GKR++G G EN  E++  R ++ E+V   GA N  D+ PILR +    
Sbjct: 190 CLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TN 249

Query: 251 LEKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDII 310
            EK + + + R D FLQ L++E+R ++V        GNTMI  LL LQ+++P YYTD II
Sbjct: 250 YEKHVKKLAGRVDEFLQSLVNEKRVEKVK-------GNTMIDHLLSLQETQPDYYTDVII 309

Query: 311 KAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKLQY 370
           K  +L M++AGTDT+ GTL WAMS LLNHP ++ KA TEI                KL Y
Sbjct: 310 KGIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPY 369

Query: 371 LQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRF 430
           LQ I+ ET RLYP+AP+L+PH ASEDC ++G+DVP+GT++LVNAWA+HRDPK+WE+P +F
Sbjct: 370 LQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKF 429

Query: 431 RPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVD 482
           +PERF + +G      K MPFG+GRR+CPG+ L QR+V L L +L+QCFEW+R+ E+ +D
Sbjct: 430 KPERF-EKKG---EDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLD 489

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023535658.18.9e-20169.13isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo][more]
XP_022936846.14.9e-19968.74isoflavone 3'-hydroxylase-like isoform X1 [Cucurbita moschata] >XP_022936847.1 i... [more]
XP_022977005.14.5e-19769.98isoflavone 3'-hydroxylase-like [Cucurbita maxima][more]
KAG7024282.16.1e-19467.57Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_038896686.18.9e-19366.34cytochrome P450 81Q32-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
W8JMU75.5e-13751.49Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1[more]
Q6WNQ84.1e-13251.70Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1[more]
Q6WNQ94.4e-12648.98Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... [more]
P931474.8e-12546.32Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2[more]
Q6WNR02.6e-12345.60Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F8L92.4e-19968.74isoflavone 3'-hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IL262.2e-19769.98isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477203 PE=3 ... [more]
A0A5A7VB122.3e-18665.43Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3BWG91.8e-18364.84LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1C4W61.8e-16257.78isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111008503 PE... [more]
Match NameE-valueIdentityDescription
AT4G37370.11.1e-12948.32cytochrome P450, family 81, subfamily D, polypeptide 8 [more]
AT4G37330.11.4e-12447.51cytochrome P450, family 81, subfamily D, polypeptide 4 [more]
AT4G37320.12.5e-12447.87cytochrome P450, family 81, subfamily D, polypeptide 5 [more]
AT4G37400.19.3e-12448.65cytochrome P450, family 81, subfamily F, polypeptide 3 [more]
AT3G28740.11.6e-12347.71Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 175..193
score: 18.4
coord: 417..427
score: 55.03
coord: 427..450
score: 33.16
coord: 341..359
score: 40.5
coord: 65..84
score: 39.13
coord: 292..309
score: 32.39
coord: 89..110
score: 18.5
coord: 382..406
score: 41.27
coord: 312..338
score: 30.01
IPR001128Cytochrome P450PRINTSPR00385P450coord: 418..427
score: 58.17
coord: 342..353
score: 44.19
coord: 303..320
score: 42.99
IPR001128Cytochrome P450PFAMPF00067p450coord: 38..470
e-value: 1.8E-89
score: 300.7
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 24..485
e-value: 2.6E-115
score: 387.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 38..481
NoneNo IPR availablePANTHERPTHR24298:SF683CYTOCHROME P450 FAMILY PROTEINcoord: 9..484
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 9..484
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 420..429

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0013457.1Sed0013457.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen