Homology
BLAST of Sed0013457 vs. NCBI nr
Match:
XP_023535658.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 710.7 bits (1833), Expect = 8.9e-201
Identity = 356/515 (69.13%), Postives = 410/515 (79.61%), Query Frame = 0
Query: 3 EIEKLSWLTHFLISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQ 62
E ++WL S +LLF L+LI K RK + PPSPP LP+IGHLHLLK PFHRVLQ
Sbjct: 21 EASTITWL-----SLSLLFTLILIHKLRKQSPRKCPPSPPSLPLIGHLHLLKQPFHRVLQ 80
Query: 63 NLSQKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAF 122
+LS K GPI LSIGSR +VVISSP A ++CFTKNDI ANRPRLL+GKY+NYNYTGVAF
Sbjct: 81 DLSNKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGVAF 140
Query: 123 AAYGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV------- 182
A+YGHHWRNMRRIATTELLSNHRL L++R ELKLW+K+LY TGE+ FV
Sbjct: 141 ASYGHHWRNMRRIATTELLSNHRLNTYLNIRVQELKLWVKNLYGATGESSDFVRLEMQSK 200
Query: 183 ----SFNTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGF 242
SFNTVMRMVSGKRYFG VE+ EE+REFR IMKE+++LSGASNP DFLPILR+ +
Sbjct: 201 LNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLSGASNPADFLPILRLFDY 260
Query: 243 GGLEKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGN-TMIQSLLCLQQSEPQYYTD 302
GLEKRM++AS RAD FLQ LID ER++R S WPE K GN TMIQSLL LQ+S+PQYY+D
Sbjct: 261 QGLEKRMVKASGRADLFLQNLIDCERERRASPWPEEKHGNKTMIQSLLSLQESQPQYYSD 320
Query: 303 DIIKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YK 362
DIIK HVLTML AGTDTTTGT+ WAMSLLLNHPTIMNKAW+EI
Sbjct: 321 DIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMNKAWSEIRECVGESRLVEEGDVSS 380
Query: 363 LQYLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDP 422
L+Y++AIIYET RL+P+APLLVPHE+SE+C IEGFD+PKGTMLLVNAWA+HRDP++WEDP
Sbjct: 381 LKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWEDP 440
Query: 423 TRFRPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEE 482
T FRPER ++WE S+KW+PFG+GRRACPGA L RVVGLTLATLIQCFEW+R+GEE
Sbjct: 441 TSFRPERLLNWES--AESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEE 500
Query: 483 EVDLSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
VDLSEG GIT+PK PLEAM R RSSM+HLLS L
Sbjct: 501 PVDLSEGTGITIPKAIPLEAMCRARSSMLHLLSLL 528
BLAST of Sed0013457 vs. NCBI nr
Match:
XP_022936846.1 (isoflavone 3'-hydroxylase-like isoform X1 [Cucurbita moschata] >XP_022936847.1 isoflavone 3'-hydroxylase-like isoform X2 [Cucurbita moschata])
HSP 1 Score: 704.9 bits (1818), Expect = 4.9e-199
Identity = 354/515 (68.74%), Postives = 407/515 (79.03%), Query Frame = 0
Query: 3 EIEKLSWLTHFLISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQ 62
E ++WL S +LLF L LI K RK + PPSPP LP+IGHLHLLK PFHRVLQ
Sbjct: 2 EANTITWL-----SLSLLFTLSLIYKLRKQSPRRCPPSPPSLPLIGHLHLLKQPFHRVLQ 61
Query: 63 NLSQKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAF 122
+LS K GPI LSIGSR +VVISSP A ++CFTKNDI ANRPRLL+GKY+NYNYTGVA
Sbjct: 62 DLSDKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGVAV 121
Query: 123 AAYGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV------- 182
A+YGHHWRNMRRIATTELLSNHRL L++R ELKLW+K+LY ETGE+ FV
Sbjct: 122 ASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWVKNLYGETGESSDFVRLEMQSK 181
Query: 183 ----SFNTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGF 242
SFNTVMRMVSGKRYFG VE+ EE+REFR IMKE++ LSGASNP DFLPILR+ +
Sbjct: 182 LNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLHLSGASNPADFLPILRLFDY 241
Query: 243 GGLEKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGN-TMIQSLLCLQQSEPQYYTD 302
GLEKRM++AS RAD FLQ LID ER++R SRWPE K GN TMIQSLL Q+S+PQYY+D
Sbjct: 242 QGLEKRMVKASGRADLFLQNLIDCERERRASRWPEEKHGNKTMIQSLLSFQESQPQYYSD 301
Query: 303 DIIKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YK 362
DIIK HVLTML AGTDTTTGT+ WAMSLLLNHPTIM+KAW+EI
Sbjct: 302 DIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMDKAWSEIRECVGESRLVEEGDVSS 361
Query: 363 LQYLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDP 422
L+Y++AIIYET RL+P+APLLVPHE+SE+C IEGFD+PKGTMLLVNAWA+HRDP++WEDP
Sbjct: 362 LKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWEDP 421
Query: 423 TRFRPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEE 482
T FRPERF++WE S+KW+PFG+GRRACPG L RVVGLTLATLIQCFEW+R+GEE
Sbjct: 422 TSFRPERFLNWEA--AESHKWVPFGVGRRACPGVALAHRVVGLTLATLIQCFEWQRVGEE 481
Query: 483 EVDLSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
+DLSEG GITMPK PL AM R RSSM+HLLS L
Sbjct: 482 PIDLSEGTGITMPKAIPLVAMCRARSSMLHLLSPL 509
BLAST of Sed0013457 vs. NCBI nr
Match:
XP_022977005.1 (isoflavone 3'-hydroxylase-like [Cucurbita maxima])
HSP 1 Score: 698.4 bits (1801), Expect = 4.5e-197
Identity = 352/503 (69.98%), Postives = 402/503 (79.92%), Query Frame = 0
Query: 15 ISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQNLSQKLGPIFFL 74
+S +LL L+LI K RK ++ PPSPP LP+IGHLHLLK PFHRVLQ+LS K GPI L
Sbjct: 20 LSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQDLSNKHGPILSL 79
Query: 75 SIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHHWRNMRR 134
SIGSR +VVISSP A ++CFTKNDI LANRPRLLAGKY+NYNYTGVAFA+YGHHWRNMRR
Sbjct: 80 SIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFASYGHHWRNMRR 139
Query: 135 IATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV-----------SFNTVMRM 194
IATTELLSNHRL L++R ELKL +K+LY TGE+ FV SFN VMRM
Sbjct: 140 IATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFVRLEMQSKLNELSFNIVMRM 199
Query: 195 VSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKRMMEASE 254
VSGKRYFG VE+ EE+REFR IMKE+++L GASNP DFLPILR+ + GLEKRM++AS
Sbjct: 200 VSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQGLEKRMVQASG 259
Query: 255 RADGFLQKLIDEERDKRVSRWPEGKDGN-TMIQSLLCLQQSEPQYYTDDIIKAHVLTMLV 314
RAD FLQ LID ERD+R SRWPE K GN TMIQSLL LQ+S+PQ+Y+DDIIK HVLTML
Sbjct: 260 RADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDDIIKGHVLTMLA 319
Query: 315 AGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKLQYLQAIIYETF 374
AGTDTTTGTL WAMSLLLNHP IMNKAW+EI L+YL AIIYET
Sbjct: 320 AGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLKYLVAIIYETL 379
Query: 375 RLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFVDWE 434
RL+P APLLVPHE+SE+C +EGFD+PKGTMLLVNAWA+HRDP+VWEDPT FRPERF++WE
Sbjct: 380 RLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTSFRPERFLNWE 439
Query: 435 GNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNGITM 492
S+KW+PFG+GRRACPGA L RVVGLTLATLIQCFEW+R+GEE +DLS+G GIT
Sbjct: 440 --TAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSQGTGITT 499
BLAST of Sed0013457 vs. NCBI nr
Match:
KAG7024282.1 (Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 688.0 bits (1774), Expect = 6.1e-194
Identity = 348/515 (67.57%), Postives = 402/515 (78.06%), Query Frame = 0
Query: 3 EIEKLSWLTHFLISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQ 62
E ++WL S +LLF L LI K RK + PPSPP LP+IGHLHLLK PFHRVLQ
Sbjct: 2 EANTITWL-----SLSLLFTLSLIYKLRKQSPRRCPPSPPSLPLIGHLHLLKQPFHRVLQ 61
Query: 63 NLSQKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAF 122
+LS K GPI LSIGSR +VVISSP A ++CFTKNDI ANRPRLL+G Y+NYNYTGVA
Sbjct: 62 DLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIAFANRPRLLSGNYMNYNYTGVAV 121
Query: 123 AAYGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV------- 182
A+YGHHWRNMRR+AT ELLSN RL L++R ELKL +K+LY TGE+ FV
Sbjct: 122 ASYGHHWRNMRRVATKELLSNQRLDTYLNIRVQELKLCVKNLYGATGESSDFVRLEMQSK 181
Query: 183 ----SFNTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGF 242
SFNTVMRMVSGKRYFG VE+ EE+REFR IMKE+++LSGASNP DFLPILR+ +
Sbjct: 182 LNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLSGASNPADFLPILRLFDY 241
Query: 243 GGLEKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGN-TMIQSLLCLQQSEPQYYTD 302
GLEKRM++AS RAD FLQ LID ER++R SRWPE K GN TMIQSLL Q+S+PQYY+D
Sbjct: 242 QGLEKRMVKASSRADLFLQNLIDCERERRSSRWPEEKHGNKTMIQSLLSFQESQPQYYSD 301
Query: 303 DIIKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YK 362
DIIK HVLTML AGTDTTTGT+ WAMSLLLNHPTIMNKAW+EI
Sbjct: 302 DIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMNKAWSEIRECVGESRLVEEGDVSS 361
Query: 363 LQYLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDP 422
L+Y++AIIYET RL+P+APLLVPHE+SE+C IEGFD+PKGTMLLVNAWA+HRDP++WEDP
Sbjct: 362 LKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWEDP 421
Query: 423 TRFRPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEE 482
T FRPERF++WE S+KW+ FG+GR ACPGA L RVVGLTLATLIQCFEW+R+GEE
Sbjct: 422 TSFRPERFLNWEA--AESHKWVLFGVGRGACPGAALAHRVVGLTLATLIQCFEWQRVGEE 481
Query: 483 EVDLSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
+DLSEG GITMPK PL AM R RSSM+HLLS L
Sbjct: 482 PIDLSEGTGITMPKAIPLVAMCRARSSMLHLLSPL 509
BLAST of Sed0013457 vs. NCBI nr
Match:
XP_038896686.1 (cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 684.1 bits (1764), Expect = 8.9e-193
Identity = 341/514 (66.34%), Postives = 398/514 (77.43%), Query Frame = 0
Query: 3 EIEKLSWLTHFLISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQ 62
E ++W FLIS LF +L+SK RK ++K LPPSPP LPVIGHLHLL++P HRVL
Sbjct: 2 EPHTITWFFPFLIS-LFLFTFILLSKLRKINHKKLPPSPPSLPVIGHLHLLRDPLHRVLH 61
Query: 63 NLSQKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAF 122
NLS K GPI LSIGSR VVV+SSP A ++CFTKNDIV ANRPRLL+GKYINYNYT V F
Sbjct: 62 NLSNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGF 121
Query: 123 AAYGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV------- 182
A YG HWRNMRRIATTELLSNHRL L++R ELKLW+K+LY FV
Sbjct: 122 APYGQHWRNMRRIATTELLSNHRLNTYLNIRVQELKLWIKNLYKLICREDNFVGVEMKSK 181
Query: 183 ----SFNTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGF 242
SFN VMRMVSGKRYFG V + EE+ EFR + KE++ELSGA+NP DFL ILR+ F
Sbjct: 182 LKELSFNIVMRMVSGKRYFGVEVVDVEEALEFRELTKELLELSGATNPADFLTILRVFDF 241
Query: 243 GGLEKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDD 302
GL KRM++AS RAD FLQKLIDE+R++R+SRWPE K G +MIQ+LL LQ+S+P YY+DD
Sbjct: 242 QGLNKRMLKASGRADVFLQKLIDEQRERRLSRWPEDKHGKSMIQTLLSLQESQPDYYSDD 301
Query: 303 IIKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKL 362
IIK HVLTML AGTDTT+GT+ W MSLLLNHPT+M KAW EI L
Sbjct: 302 IIKGHVLTMLAAGTDTTSGTIEWTMSLLLNHPTVMKKAWIEIRDCVGENRMVEEADVSNL 361
Query: 363 QYLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPT 422
+Y++AIIYET RL+P APLLVPHE+SEDC IEGF+VPKGTMLLVNAWA+HRDPKVWEDP
Sbjct: 362 RYMEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEVPKGTMLLVNAWAIHRDPKVWEDPN 421
Query: 423 RFRPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEE 482
FRPERF++WEG SYKW+PFGMGRRACPGA L QRVVGLTLATLIQCFEW+++ E++
Sbjct: 422 SFRPERFLNWEG--VESYKWIPFGMGRRACPGAALAQRVVGLTLATLIQCFEWEKVDEQQ 481
Query: 483 VDLSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
+DLSEG+GITMPK L AM + R+SM+HLLS L
Sbjct: 482 IDLSEGSGITMPKAKALVAMCKPRNSMLHLLSCL 512
BLAST of Sed0013457 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 489.2 bits (1258), Expect = 5.5e-137
Identity = 260/505 (51.49%), Postives = 341/505 (67.52%), Query Frame = 0
Query: 14 LISTTLLFILVLISKFRKP---HYKSLPPSPPY-LPVIGHLHLLKNPFHRVLQNLSQKLG 73
L+ T L +L+ IS P ++LPPSP LPVIGHLHL+ HR L +LS+K G
Sbjct: 6 LLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYG 65
Query: 74 PIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHHW 133
+F L +G+R V+V+SSPAAA++CFTKNDIV ANRP + GKYI YNYT + + YG HW
Sbjct: 66 SVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHW 125
Query: 134 RNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV---------SFNTV 193
RN+RR+A E+ S L LS+R E+K L LY +G++ G V SFN
Sbjct: 126 RNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVT 185
Query: 194 MRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKRMME 253
MRMV+GKRYFG V++ +E++ FR ++ E+ E +GASNP DF+P LR I F EK++ +
Sbjct: 186 MRMVAGKRYFGQDVDS-DEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVSK 245
Query: 254 ASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDIIKAHVLTM 313
S+ D FLQ+LI E R K+ TMI LL LQ+S+P+YYTD IIK ++ +
Sbjct: 246 ISQEMDAFLQRLIHESR--------INKNNVTMIDHLLSLQESQPEYYTDQIIKGIIMVL 305
Query: 314 LVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEISY--------------KLQYLQAIIYE 373
L+AGTDT+ T+ WAMSLLLNHP + KA TEI KL YL II E
Sbjct: 306 LLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISE 365
Query: 374 TFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFVD 433
TFRL P AP+LVPHE+S+DC ++G+DVPKGT+LLVNAWA+HRDP+ W++PT F+PER
Sbjct: 366 TFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPER--- 425
Query: 434 WEGNIE-SSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNG 491
G +E K MPFGMGRR+CPG+ L QRVVGLTL LIQCFEWKRIGE ++D++EG+G
Sbjct: 426 -HGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSG 485
BLAST of Sed0013457 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 473.0 bits (1216), Expect = 4.1e-132
Identity = 259/501 (51.70%), Postives = 329/501 (65.67%), Query Frame = 0
Query: 10 LTHFLIS--TTLLFILVLISKF--RKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQNLS 69
+T F +S +L F+++ + F +K+LPP P LP+IG+LH LK P H LS
Sbjct: 1 MTTFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLS 60
Query: 70 QKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAY 129
QK G IF L GSR VVV+SS AQ+CFTKNDIVLANRP L GKYI YN T VA + Y
Sbjct: 61 QKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPY 120
Query: 130 GHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDL----YN-----ETGENRGFVS 189
G HWRN+RRI + E+LS+HRL + L +R E+ ++ L YN E ++
Sbjct: 121 GDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMT 180
Query: 190 FNTVMRMVSGKRYFG--AGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGL 249
FNT+MRMVSGKRY+G V + EE+R FRGI+KE+V L GA+N DFL LR F GL
Sbjct: 181 FNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGL 240
Query: 250 EKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDIIK 309
EKR+ + S+R D FLQ LIDE R + ++ NTMI LL QQS+P+YYTD IIK
Sbjct: 241 EKRLKKISKRTDAFLQGLIDEHRFGK-------RNSNTMIDHLLTQQQSQPEYYTDQIIK 300
Query: 310 AHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKLQYL 369
++ ML+AGTDT++ T+ WAMS LLNHP IM KA E+ KL YL
Sbjct: 301 GLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYL 360
Query: 370 QAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFR 429
Q+I+YET RL+ APLLVPH +SED ++ G+++P+ T+L+VNAW +HRDP +W DPT F+
Sbjct: 361 QSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFK 420
Query: 430 PERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDL 482
PERF + EG + K + FG+GRRACPG L QR GLTL LIQCFEWKRIGEE++D+
Sbjct: 421 PERF-EKEGEVN---KLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDM 480
BLAST of Sed0013457 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 453.0 bits (1164), Expect = 4.4e-126
Identity = 241/492 (48.98%), Postives = 314/492 (63.82%), Query Frame = 0
Query: 14 LISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQNLSQKLGPIFF 73
L+S + + + ++ K K+LPP PP +P+IG+LH LK+P HR LSQ G IF
Sbjct: 7 LLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFS 66
Query: 74 LSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHHWRNMR 133
L GSR VVV+SSP+ A +CFTKNDI+LANRPR L GKYI YNYT + A+YG HWRN+R
Sbjct: 67 LWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLR 126
Query: 134 RIATTELLSNHRLGNDLSVRAHEL-KLWLKDLYNETGENRGF-----------VSFNTVM 193
RI T ++LSN+RL + L VR E +L K L + E GF ++FN +M
Sbjct: 127 RITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMM 186
Query: 194 RMVSGKRYFG--AGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKRMM 253
RM+SGKRY+G V + EE+++FR I+ E++ L GA+N DFLP+LR++ LEKR
Sbjct: 187 RMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCK 246
Query: 254 EASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDIIKAHVLT 313
++R++ FL+ LI+E R + DG TMI LL L +S+P+YY+D +IK +
Sbjct: 247 RIAKRSNAFLEGLIEEHRRGNIH-----SDGGTMIDHLLKLSESQPEYYSDHLIKGLIQG 306
Query: 314 MLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKLQYLQAIIY 373
ML+AGTDT+ T+ W MS LLNHP ++ KA E+ KL YLQ II
Sbjct: 307 MLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIIS 366
Query: 374 ETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFV 433
ET RL+P APLL+PH +SEDCTI F+VPK T++L N W +HRDPK W D F+PERF
Sbjct: 367 ETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERF- 426
Query: 434 DWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNG 478
E K M FG+GRRACPG L QR VG T+ LIQCFEW+R EE++D+ EG G
Sbjct: 427 ---EKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKG 486
BLAST of Sed0013457 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 449.5 bits (1155), Expect = 4.8e-125
Identity = 233/503 (46.32%), Postives = 328/503 (65.21%), Query Frame = 0
Query: 4 IEKLSWLTHFLISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQN 63
+E LS L++ + L FI ++ + RK +K+LPP PP LP+IG+LH LK P HR +
Sbjct: 1 MEILSLLSYSVFYLALFFIFNIVIRARK--FKNLPPGPPSLPIIGNLHHLKRPLHRTFKG 60
Query: 64 LSQKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFA 123
LS+K G +F L GSR VVV+SS + QQCFTKND+VLANRPR L+GKYI YNYT +
Sbjct: 61 LSEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGST 120
Query: 124 AYGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRG---------F 183
+YG HWRN+RRI ++LSNHR+ + +R E + + L +++ N
Sbjct: 121 SYGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYD 180
Query: 184 VSFNTVMRMVSGKRYFGAGVENG--EESREFRGIMKEIVELSGASNPVDFLPILRMIGFG 243
++FN +MRM+SGKRY+G + +E+ +FR ++ E+++LSGA+N DF+P+LR + F
Sbjct: 181 MTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFE 240
Query: 244 GLEKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDI 303
LEKR+ + S + D FL+ LI+E R K+ + NTMI LL LQ S+P+YYTD I
Sbjct: 241 NLEKRLKDISGKTDAFLRGLIEEHRTKK-------ERANTMIDHLLNLQDSQPEYYTDQI 300
Query: 304 IKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEISY--------------KLQ 363
IK L ML+AGTD++ TL W+MS LLNHP ++ K E+ KL
Sbjct: 301 IKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLT 360
Query: 364 YLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTR 423
YL+ +I ET RLY APLL+PH S++C I G+ VP+ T++L+NAWA+HRDP++W + T
Sbjct: 361 YLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATT 420
Query: 424 FRPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEV 482
F+PERF + +G +E K + FGMGRRACPG L R + +TLA LIQCF+WK I +++
Sbjct: 421 FKPERF-EKKGELE---KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKI 480
BLAST of Sed0013457 vs. ExPASy Swiss-Prot
Match:
Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)
HSP 1 Score: 443.7 bits (1140), Expect = 2.6e-123
Identity = 228/500 (45.60%), Postives = 328/500 (65.60%), Query Frame = 0
Query: 7 LSWLTHFLISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQNLSQ 66
LS+L + L ++ FI+ L+ + RK +K+LPP P LP+IG+LH LK P +R + L++
Sbjct: 4 LSYLCYSLFYLSIFFIIRLLFQSRK--FKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTE 63
Query: 67 KLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYG 126
K G + L GSR VVV+SS + Q+CFTKND+VLANRPR L+GKYI YNYT + +YG
Sbjct: 64 KYGNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYG 123
Query: 127 HHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGEN--------RGF-VSF 186
HWRN+RRI + ++LSNHR+ N +R E + +K L ++ R F ++F
Sbjct: 124 EHWRNLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTF 183
Query: 187 NTVMRMVSGKRYFG--AGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLE 246
N +MRM+SGKRY+G + +E+ +FR ++ E+++LSGA+N DF+P+L+ + F LE
Sbjct: 184 NNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLE 243
Query: 247 KRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDIIKA 306
KR+ E+ D FL L+ E+R K+ + NTMI LL +Q+S+P+YYTD IIK
Sbjct: 244 KRVKRIGEKNDVFLSGLLQEQRSKK-------ERTNTMIDHLLNMQESQPEYYTDTIIKG 303
Query: 307 HVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEISY--------------KLQYLQ 366
L ML+AGTD++ TL W MS +LN+P ++ K E+ KL YL+
Sbjct: 304 LCLAMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLR 363
Query: 367 AIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRP 426
+IYET RLY APLL+PH +++C + G+ VP+ T++L+NAWA+HRDP+ W + T F+P
Sbjct: 364 NVIYETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKP 423
Query: 427 ERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLS 482
ERF D +G +E K + FGMGRRACPG L R + +TLA L+QCF+WKRI +E++D+S
Sbjct: 424 ERF-DKKGELE---KMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMS 483
BLAST of Sed0013457 vs. ExPASy TrEMBL
Match:
A0A6J1F8L9 (isoflavone 3'-hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443310 PE=3 SV=1)
HSP 1 Score: 704.9 bits (1818), Expect = 2.4e-199
Identity = 354/515 (68.74%), Postives = 407/515 (79.03%), Query Frame = 0
Query: 3 EIEKLSWLTHFLISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQ 62
E ++WL S +LLF L LI K RK + PPSPP LP+IGHLHLLK PFHRVLQ
Sbjct: 2 EANTITWL-----SLSLLFTLSLIYKLRKQSPRRCPPSPPSLPLIGHLHLLKQPFHRVLQ 61
Query: 63 NLSQKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAF 122
+LS K GPI LSIGSR +VVISSP A ++CFTKNDI ANRPRLL+GKY+NYNYTGVA
Sbjct: 62 DLSDKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGVAV 121
Query: 123 AAYGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV------- 182
A+YGHHWRNMRRIATTELLSNHRL L++R ELKLW+K+LY ETGE+ FV
Sbjct: 122 ASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWVKNLYGETGESSDFVRLEMQSK 181
Query: 183 ----SFNTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGF 242
SFNTVMRMVSGKRYFG VE+ EE+REFR IMKE++ LSGASNP DFLPILR+ +
Sbjct: 182 LNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLHLSGASNPADFLPILRLFDY 241
Query: 243 GGLEKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGN-TMIQSLLCLQQSEPQYYTD 302
GLEKRM++AS RAD FLQ LID ER++R SRWPE K GN TMIQSLL Q+S+PQYY+D
Sbjct: 242 QGLEKRMVKASGRADLFLQNLIDCERERRASRWPEEKHGNKTMIQSLLSFQESQPQYYSD 301
Query: 303 DIIKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YK 362
DIIK HVLTML AGTDTTTGT+ WAMSLLLNHPTIM+KAW+EI
Sbjct: 302 DIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMDKAWSEIRECVGESRLVEEGDVSS 361
Query: 363 LQYLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDP 422
L+Y++AIIYET RL+P+APLLVPHE+SE+C IEGFD+PKGTMLLVNAWA+HRDP++WEDP
Sbjct: 362 LKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWEDP 421
Query: 423 TRFRPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEE 482
T FRPERF++WE S+KW+PFG+GRRACPG L RVVGLTLATLIQCFEW+R+GEE
Sbjct: 422 TSFRPERFLNWEA--AESHKWVPFGVGRRACPGVALAHRVVGLTLATLIQCFEWQRVGEE 481
Query: 483 EVDLSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
+DLSEG GITMPK PL AM R RSSM+HLLS L
Sbjct: 482 PIDLSEGTGITMPKAIPLVAMCRARSSMLHLLSPL 509
BLAST of Sed0013457 vs. ExPASy TrEMBL
Match:
A0A6J1IL26 (isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477203 PE=3 SV=1)
HSP 1 Score: 698.4 bits (1801), Expect = 2.2e-197
Identity = 352/503 (69.98%), Postives = 402/503 (79.92%), Query Frame = 0
Query: 15 ISTTLLFILVLISKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQNLSQKLGPIFFL 74
+S +LL L+LI K RK ++ PPSPP LP+IGHLHLLK PFHRVLQ+LS K GPI L
Sbjct: 20 LSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQDLSNKHGPILSL 79
Query: 75 SIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHHWRNMRR 134
SIGSR +VVISSP A ++CFTKNDI LANRPRLLAGKY+NYNYTGVAFA+YGHHWRNMRR
Sbjct: 80 SIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFASYGHHWRNMRR 139
Query: 135 IATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV-----------SFNTVMRM 194
IATTELLSNHRL L++R ELKL +K+LY TGE+ FV SFN VMRM
Sbjct: 140 IATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFVRLEMQSKLNELSFNIVMRM 199
Query: 195 VSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKRMMEASE 254
VSGKRYFG VE+ EE+REFR IMKE+++L GASNP DFLPILR+ + GLEKRM++AS
Sbjct: 200 VSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQGLEKRMVQASG 259
Query: 255 RADGFLQKLIDEERDKRVSRWPEGKDGN-TMIQSLLCLQQSEPQYYTDDIIKAHVLTMLV 314
RAD FLQ LID ERD+R SRWPE K GN TMIQSLL LQ+S+PQ+Y+DDIIK HVLTML
Sbjct: 260 RADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDDIIKGHVLTMLA 319
Query: 315 AGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKLQYLQAIIYETF 374
AGTDTTTGTL WAMSLLLNHP IMNKAW+EI L+YL AIIYET
Sbjct: 320 AGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLKYLVAIIYETL 379
Query: 375 RLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFVDWE 434
RL+P APLLVPHE+SE+C +EGFD+PKGTMLLVNAWA+HRDP+VWEDPT FRPERF++WE
Sbjct: 380 RLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTSFRPERFLNWE 439
Query: 435 GNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNGITM 492
S+KW+PFG+GRRACPGA L RVVGLTLATLIQCFEW+R+GEE +DLS+G GIT
Sbjct: 440 --TAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSQGTGITT 499
BLAST of Sed0013457 vs. ExPASy TrEMBL
Match:
A0A5A7VB12 (Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002950 PE=3 SV=1)
HSP 1 Score: 661.8 bits (1706), Expect = 2.3e-186
Identity = 335/512 (65.43%), Postives = 396/512 (77.34%), Query Frame = 0
Query: 10 LTHFLISTTLLFILVLISKFRK-PHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQNLSQKL 69
+T F +S L+ L+L+SK RK + K LPP+PP LP+IGHLHLLK PFHRVL NLS K
Sbjct: 14 ITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHNLSNKY 73
Query: 70 GPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHH 129
GPI L+IGSR V+V+SSP A ++CFTKNDIV ANRPRL++GKYINYNYT V FA YG H
Sbjct: 74 GPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFAPYGQH 133
Query: 130 WRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV-----------SF 189
WRNMRRIATTELLSNHRL L++R ELKLW+K+ + G FV SF
Sbjct: 134 WRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKLKELSF 193
Query: 190 NTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKR 249
NTVMRM+SGKRYFG VE+ EE+ EFR IMKE++ELSGA+N DFL ILR+ G++KR
Sbjct: 194 NTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLEGVKKR 253
Query: 250 MMEASERADGFLQKLIDEERDKRVSRWPEGKD---GNTMIQSLLCLQQSEPQYYTDDIIK 309
MM+AS RAD FLQKLIDEER KRVSRWPE K +MI++LL LQ+S+PQYY+DDIIK
Sbjct: 254 MMKASGRADVFLQKLIDEERKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYYSDDIIK 313
Query: 310 AHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKLQYL 369
HVLTML AGTDTT+GT+ WAMSLLLNHP M KAW EI +L+YL
Sbjct: 314 GHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVSELKYL 373
Query: 370 QAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFR 429
+AIIYET R++P APLLVPHE SEDC+IEGF+VPKGTMLLVNAW +HRDPKVWEDPT F+
Sbjct: 374 EAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWEDPTSFQ 433
Query: 430 PERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGE-EEVD 489
PERF++WEG +YKW+PFGMGRRACPG L QR +GLTLATLIQCFEWK++ E E++D
Sbjct: 434 PERFLNWEG--VETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQEQID 493
Query: 490 LSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
LSEG+GITMPK LEAM + R+SM+HLL+QL
Sbjct: 494 LSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 523
BLAST of Sed0013457 vs. ExPASy TrEMBL
Match:
A0A1S3BWG9 (LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493891 PE=3 SV=1)
HSP 1 Score: 652.1 bits (1681), Expect = 1.8e-183
Identity = 332/512 (64.84%), Postives = 394/512 (76.95%), Query Frame = 0
Query: 10 LTHFLISTTLLFILVLISKFRK-PHYKSLPPSPPYLPVIGHLHLLKNPFHRVLQNLSQKL 69
+T F +S L+ L+L+SK RK + K LPP+PP LP+IGHLHLLK PFHRVL NLS K
Sbjct: 11 ITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRVLHNLSNKY 70
Query: 70 GPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHH 129
GPI L+IGSR V+V+SSP A ++CFTKNDIV ANRPRL++GKYINYNYT V FA YG H
Sbjct: 71 GPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFAPYGQH 130
Query: 130 WRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV-----------SF 189
WRNMRRIATTELLSNHRL L++R ELKLW+K+ + G FV SF
Sbjct: 131 WRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKLKELSF 190
Query: 190 NTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKR 249
NTVMRM+SGKRYFG VE+ EE+ EFR IMKE++ELSGA+N DFL ILR+ G++KR
Sbjct: 191 NTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLEGVKKR 250
Query: 250 MMEASERADGFLQKLIDEERDKRVSRWPEGKD---GNTMIQSLLCLQQSEPQYYTDDIIK 309
MM+AS RAD FLQKLIDEER K+ RWPE K +MI++LL LQ+S+PQYY+DDIIK
Sbjct: 251 MMKASGRADVFLQKLIDEER-KKEFRWPEEKQKQAKTSMIRTLLSLQESQPQYYSDDIIK 310
Query: 310 AHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKLQYL 369
HVLTML AGTDTT+GT+ WAMSLLLNHP M KAW EI +L+YL
Sbjct: 311 GHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVSELKYL 370
Query: 370 QAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFR 429
+AIIYET R++P APLLVPHE SEDC+IEGF+VPKGTMLLVNAW +HRDPKVWEDPT F+
Sbjct: 371 EAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWEDPTSFQ 430
Query: 430 PERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGE-EEVD 489
PERF++WEG +YKW+PFGMGRRACPG L QR +GLTLATLIQCFEWK++ E E++D
Sbjct: 431 PERFLNWEG--VETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQEQID 490
Query: 490 LSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
LSEG+GITMPK LEAM + R+SM+HLL+QL
Sbjct: 491 LSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 519
BLAST of Sed0013457 vs. ExPASy TrEMBL
Match:
A0A6J1C4W6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111008503 PE=3 SV=1)
HSP 1 Score: 582.4 bits (1500), Expect = 1.8e-162
Identity = 297/514 (57.78%), Postives = 369/514 (71.79%), Query Frame = 0
Query: 4 IEKLSWLTHFLISTTLLFILVLI--SKFRKPHYKSLPPSPPYLPVIGHLHLLKNPFHRVL 63
+E++ + FL+ LLF L+ + KF+K K+LPP PP LPVIGHLHLL+ PFH++L
Sbjct: 1 MEEILQFSRFLVPLALLFALIHVFNFKFKKVSSKNLPPXPPSLPVIGHLHLLREPFHQML 60
Query: 64 QNLSQKLGPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVA 123
QNLSQ+ GPI LS+G RSVVV+SSP AAQ+CFTKNDIV ANRPR+++GK +NYNYT V
Sbjct: 61 QNLSQRYGPILSLSLGFRSVVVVSSPIAAQECFTKNDIVFANRPRMISGKILNYNYTTVG 120
Query: 124 FAAYGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV------ 183
AYG HWR MRRIATTELLS HRL + +++R E+KLWLK+LY TG+N V
Sbjct: 121 ATAYGQHWRIMRRIATTELLSTHRLISFINLRQEEVKLWLKNLYRITGQNNVRVEIRSRL 180
Query: 184 ---SFNTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFG 243
SFN V+RM++GKRYFG V++ EE RE + +MKE+ LSG S P DFL +LR+I +
Sbjct: 181 TELSFNIVLRMMTGKRYFGVEVKDSEEERELKKVMKEVSLLSGMSYPADFLSVLRLIDYQ 240
Query: 244 GLEKRMMEASERADGFLQKLIDEERDKRVS-RWPEGKDGNTMIQSLLCLQQSEPQYYTDD 303
G KR+ + ADGF+Q LID R+ + S + ++ MI ++L LQ S+P YY D
Sbjct: 241 GFRKRLWKVWAGADGFVQXLIDGRREMKASCSFSLEENWKNMIDTMLSLQDSDPDYYKDH 300
Query: 304 IIKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKL 363
IIK +LT+LVAGTDTT GT+ W MSLLLNHPTIM KAW+EI+ L
Sbjct: 301 IIKGQILTLLVAGTDTTAGTIEWGMSLLLNHPTIMKKAWSEINDYVGEQRLVEEADLANL 360
Query: 364 QYLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPT 423
YLQAII ET+RL+P P+LVP E+SEDCTI G+DVPKGTML+VN WA+HRDP+VW+DPT
Sbjct: 361 PYLQAIINETYRLFPALPILVPRESSEDCTIGGYDVPKGTMLVVNMWAIHRDPQVWQDPT 420
Query: 424 RFRPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEE 483
RF+PERF + G + K +PFGMGRRACPGA L RVVGLTLATLIQCFEW RI E
Sbjct: 421 RFQPERFEN--GEVVEVNKLLPFGMGRRACPGAGLAHRVVGLTLATLIQCFEWDRIDPNE 480
Query: 484 VDLSEGNGITMPKTTPLEAMFRVRSSMVHLLSQL 492
+DL +GNG+TMPK PLEAM + R M+ LLS L
Sbjct: 481 IDLXQGNGLTMPKAHPLEAMCKPRGPMISLLSHL 512
BLAST of Sed0013457 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 461.1 bits (1185), Expect = 1.1e-129
Identity = 244/505 (48.32%), Postives = 338/505 (66.93%), Query Frame = 0
Query: 13 FLISTTLLFILVLISKF-RKPHYKSLPPSPPY-LPVIGHLHLLKNPFHRVLQNLSQKL-- 72
F I +L ++ LI K RKP +LPPSP + LPVIGHL LLK P HR +LSQ L
Sbjct: 8 FSILFVVLSLIYLIGKLKRKP---NLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNN 67
Query: 73 GPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHH 132
PIF L +G+R V V SS + A++CFTKND+VLANRP + K++ Y+YT + A+YG H
Sbjct: 68 APIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDH 127
Query: 133 WRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGEN---------RGFVSFNT 192
WRN+RRI + E+ SNHRL + LS+R E++ + L + ++FN
Sbjct: 128 WRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNN 187
Query: 193 VMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKRMM 252
++RMV+GKRY+G GVE+ E++ R ++ ++V +GA N VD+LP+LR++ E R+
Sbjct: 188 ILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVK 247
Query: 253 EASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDIIKAHVLT 312
+ + R D FLQ L+DE+R+ + + GNTMI LL LQ+S+P Y+TD IIK ++L
Sbjct: 248 KLAGRLDEFLQGLVDEKREAK-------EKGNTMIDHLLTLQESQPDYFTDRIIKGNMLA 307
Query: 313 MLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEISYK--------------LQYLQAIIY 372
+++AGTDT+ TL WA+S +LNHP ++NKA EI K L YLQ I+
Sbjct: 308 LILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVS 367
Query: 373 ETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFV 432
ET RLYP AP+L+PH ASEDC + G+D+P+GT+LL N WA+HRDP++W+DP F+PERF
Sbjct: 368 ETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERF- 427
Query: 433 DWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNG 491
+ EG + K MPFG+GRRACPG+ L R++ LTL +LIQC EW++IG EEVD+SEG G
Sbjct: 428 EKEGEAQ---KLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIG-EEVDMSEGKG 487
BLAST of Sed0013457 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 444.1 bits (1141), Expect = 1.4e-124
Identity = 239/503 (47.51%), Postives = 321/503 (63.82%), Query Frame = 0
Query: 14 LISTTLLFILVLISKFRKPHYKSLPPSPPY-LPVIGHLHLLKNPFHRVLQNLSQKLG--P 73
++S LF L + R +LPPSP LPVIGHLHLLK P HR +LS+ +G P
Sbjct: 8 ILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAP 67
Query: 74 IFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHHWR 133
+F L +G+R V VISS + A++CFTKND+VLANRP+ K++ YN T + A+YG HWR
Sbjct: 68 VFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWR 127
Query: 134 NMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV-----------SFNT 193
N+RRIA E+ S HRL + L +R E++ + L ++ GFV + NT
Sbjct: 128 NLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDS--LHGFVEVEMKTLLTNLASNT 187
Query: 194 VMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKRMM 253
+RM++GKRYFG E+ ++++ + ++ E V +GA NP+D+L ILR + EKR+
Sbjct: 188 TIRMLAGKRYFG---EDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKRIK 247
Query: 254 EASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDIIKAHVLT 313
R D FLQKL+DE+R E + G TMI LL LQ +P YYTD IIK +LT
Sbjct: 248 NLGNRFDTFLQKLVDEKR-------AEKEKGETMIDHLLALQDIQPDYYTDVIIKGIILT 307
Query: 314 MLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEISYK--------------LQYLQAIIY 373
+++AGTDT++ TL WAMS LLNHP I+ KA EI K L YLQ+I+
Sbjct: 308 LIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVL 367
Query: 374 ETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFV 433
ET R+YP PLL+PH +SEDC + G+D+P GTM+L NAWAMHRDP+VWEDP F+PERF
Sbjct: 368 ETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERF- 427
Query: 434 DWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNG 489
+ EG E K + FGMGRRACPGA L R++ L +L+QCFEW+R+GE+ VD++E G
Sbjct: 428 EKEGEAE---KLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKG 487
BLAST of Sed0013457 vs. TAIR 10
Match:
AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )
HSP 1 Score: 443.4 bits (1139), Expect = 2.5e-124
Identity = 236/493 (47.87%), Postives = 320/493 (64.91%), Query Frame = 0
Query: 18 TLLFILVLISKFRKPHYKSLPPSPPY-LPVIGHLHLLKNPFHRVLQNLSQKLG--PIFFL 77
T+ L+L RKP +LPPSP Y LPVIGHLHLLK P HR ++S+ LG PIF L
Sbjct: 15 TISIKLLLTKSNRKP---NLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPIFHL 74
Query: 78 SIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAAYGHHWRNMRR 137
+G+R V VISS + A++CFTKND+VLANRP ++ K++ YN+T + A+YG HWRN+RR
Sbjct: 75 RLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRNLRR 134
Query: 138 IATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV-----------SFNTVMRM 197
IA E+ S+HR+ S+R E++ + L ++ GFV +FN ++ M
Sbjct: 135 IAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDS--LHGFVEVELKSLLTNLAFNNIIMM 194
Query: 198 VSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGGLEKRMMEASE 257
V+GKRY+G G E+ +E++ R ++ EI+ +G+ N D+LP + + E +
Sbjct: 195 VAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFENQTKILGN 254
Query: 258 RADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDIIKAHVLTMLVA 317
R D LQKL+DE+R E + G T+I LL Q++EP+YYTD IIK +L +++A
Sbjct: 255 RLDRVLQKLVDEKR-------AEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLA 314
Query: 318 GTDTTTGTLVWAMSLLLNHPTIMNKAWTEISYK--------------LQYLQAIIYETFR 377
GTDT++ TL WAMS LLNHP I+ KA EI K L YLQ I+ ET R
Sbjct: 315 GTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLR 374
Query: 378 LYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFVDWEG 437
LYP PLL+PH +S++C + G+D+P+ T+LL N WAMHRDP +WE+P RF+PERF + EG
Sbjct: 375 LYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERF-EKEG 434
Query: 438 NIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNGITMP 483
+ K MPFGMGRRACPGA LG+R+V L L LIQ FEW+R+G E VD++EG GITMP
Sbjct: 435 ---EARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMP 489
BLAST of Sed0013457 vs. TAIR 10
Match:
AT4G37400.1 (cytochrome P450, family 81, subfamily F, polypeptide 3 )
HSP 1 Score: 441.4 bits (1134), Expect = 9.3e-124
Identity = 234/481 (48.65%), Postives = 314/481 (65.28%), Query Frame = 0
Query: 36 SLPPSPPY-LPVIGHLHLLKNPFHRVLQNLSQKLGPIFFLSIGSRSVVVISSPAAAQQCF 95
+LPPSPPY LP++GH +LLK P HR+ LS+ GPIF L GSR VVISS + A QCF
Sbjct: 29 NLPPSPPYSLPILGHHNLLKPPVHRLFHRLSKTHGPIFSLQFGSRRAVVISSSSLATQCF 88
Query: 96 T-KNDIVLANRPRLLAGKYINYNYTGVAFAAYGHHWRNMRRIATTELLSNHRLGNDLSVR 155
T +NDI+L+NRP L KY+ YNYT V A YG HWRN+RRI + E+LS++RL N L +R
Sbjct: 89 TGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGDHWRNLRRICSLEILSSNRLTNFLHIR 148
Query: 156 AHELKLWLKDLYNETGEN------RGFVSFNTVMRMVSGKRYFGAGVENGEESREFRGIM 215
E+ L L + + ++FN ++RMV+GKRY+G V N EE+ F+ ++
Sbjct: 149 KDEIHRMLTRLSRDVNKEIELEPLLSDLTFNNIVRMVTGKRYYGDEVHNEEEANVFKKLV 208
Query: 216 KEIVELSGASNPVDFLPILRMIGFGGLEKRMMEASERADGFLQKLIDE-ERDKRVSRWPE 275
+I + SGA +P D+LP ++M G G EK++ +E D LQ+L++E +RD
Sbjct: 209 ADINDCSGARHPGDYLPFMKMFG-GSFEKKVKALAEAMDEILQRLLEECKRD-------- 268
Query: 276 GKDGNTMIQSLLCLQQSEPQYYTDDIIKAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIM 335
KDGNTM+ LL LQQ+EP+YYTD IK +L M++AGTDT+ TL WAMS LLNHP +
Sbjct: 269 -KDGNTMVNHLLSLQQNEPEYYTDVTIKGLMLGMMIAGTDTSAVTLEWAMSSLLNHPEAL 328
Query: 336 NKAWTEISYK--------------LQYLQAIIYETFRLYPIAPLLVPHEASEDCTIEGFD 395
KA EI K L YLQ I+ ETFRLYP APLLVP +ED + G+D
Sbjct: 329 EKAKLEIDEKIGQERLIDEPDIANLPYLQNIVSETFRLYPAAPLLVPRSPTEDIKVGGYD 388
Query: 396 VPKGTMLLVNAWAMHRDPKVWEDPTRFRPERFVDWE--GNIESSYKWMPFGMGRRACPGA 455
VP+GTM++VNAWA+HRDP++W +P +F+PERF E G E +K MPFG GRR+CPGA
Sbjct: 389 VPRGTMVMVNAWAIHRDPELWNEPEKFKPERFNGGEGGGRGEDVHKLMPFGNGRRSCPGA 448
Query: 456 VLGQRVVGLTLATLIQCFEWKRIGEEEVDLSEGNGITMPKTTPLEAMFRVRSSMVHLLSQ 492
LGQ++V L L +LIQCF+W+++ E +D++E G+ M K PL A+ + R M L +
Sbjct: 449 GLGQKIVTLALGSLIQCFDWQKVNGEAIDMTETPGMAMRKKIPLSALCQSRPIMSKLQAH 499
BLAST of Sed0013457 vs. TAIR 10
Match:
AT3G28740.1 (Cytochrome P450 superfamily protein )
HSP 1 Score: 440.7 bits (1132), Expect = 1.6e-123
Identity = 240/503 (47.71%), Postives = 325/503 (64.61%), Query Frame = 0
Query: 11 THFLISTTLLFILVLISKF---RKPHYKSLPPSPPY-LPVIGHLHLLKNPFHRVLQNLSQ 70
T +LI + F + L K +P +LPPSP P+IGHLHLLK P HR +LS+
Sbjct: 10 TIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSE 69
Query: 71 KL--GPIFFLSIGSRSVVVISSPAAAQQCFTKNDIVLANRPRLLAGKYINYNYTGVAFAA 130
L IF LS+GSR V V+SS A A++CFTKND+VLANRP L GK+I YN T + AA
Sbjct: 70 SLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAA 129
Query: 131 YGHHWRNMRRIATTELLSNHRLGNDLSVRAHELKLWLKDLYNETGENRGFV--------- 190
YG WRN+RRI T E+ S+ RL + +S+R E++ + L + GFV
Sbjct: 130 YGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNS--QHGFVKVEMKPLFM 189
Query: 191 --SFNTVMRMVSGKRYFGAGVENGEESREFRGIMKEIVELSGASNPVDFLPILRMIGFGG 250
+ N ++RMV+GKR++G G EN E++ R ++ E+V GA N D+ PILR +
Sbjct: 190 CLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TN 249
Query: 251 LEKRMMEASERADGFLQKLIDEERDKRVSRWPEGKDGNTMIQSLLCLQQSEPQYYTDDII 310
EK + + + R D FLQ L++E+R ++V GNTMI LL LQ+++P YYTD II
Sbjct: 250 YEKHVKKLAGRVDEFLQSLVNEKRVEKVK-------GNTMIDHLLSLQETQPDYYTDVII 309
Query: 311 KAHVLTMLVAGTDTTTGTLVWAMSLLLNHPTIMNKAWTEIS--------------YKLQY 370
K +L M++AGTDT+ GTL WAMS LLNHP ++ KA TEI KL Y
Sbjct: 310 KGIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPY 369
Query: 371 LQAIIYETFRLYPIAPLLVPHEASEDCTIEGFDVPKGTMLLVNAWAMHRDPKVWEDPTRF 430
LQ I+ ET RLYP+AP+L+PH ASEDC ++G+DVP+GT++LVNAWA+HRDPK+WE+P +F
Sbjct: 370 LQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKF 429
Query: 431 RPERFVDWEGNIESSYKWMPFGMGRRACPGAVLGQRVVGLTLATLIQCFEWKRIGEEEVD 482
+PERF + +G K MPFG+GRR+CPG+ L QR+V L L +L+QCFEW+R+ E+ +D
Sbjct: 430 KPERF-EKKG---EDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLD 489
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_023535658.1 | 8.9e-201 | 69.13 | isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo] | [more] |
XP_022936846.1 | 4.9e-199 | 68.74 | isoflavone 3'-hydroxylase-like isoform X1 [Cucurbita moschata] >XP_022936847.1 i... | [more] |
XP_022977005.1 | 4.5e-197 | 69.98 | isoflavone 3'-hydroxylase-like [Cucurbita maxima] | [more] |
KAG7024282.1 | 6.1e-194 | 67.57 | Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
XP_038896686.1 | 8.9e-193 | 66.34 | cytochrome P450 81Q32-like [Benincasa hispida] | [more] |
Match Name | E-value | Identity | Description | |
W8JMU7 | 5.5e-137 | 51.49 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
Q6WNQ8 | 4.1e-132 | 51.70 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
Q6WNQ9 | 4.4e-126 | 48.98 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
P93147 | 4.8e-125 | 46.32 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
Q6WNR0 | 2.6e-123 | 45.60 | Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1F8L9 | 2.4e-199 | 68.74 | isoflavone 3'-hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... | [more] |
A0A6J1IL26 | 2.2e-197 | 69.98 | isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477203 PE=3 ... | [more] |
A0A5A7VB12 | 2.3e-186 | 65.43 | Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... | [more] |
A0A1S3BWG9 | 1.8e-183 | 64.84 | LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103... | [more] |
A0A6J1C4W6 | 1.8e-162 | 57.78 | isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111008503 PE... | [more] |
Match Name | E-value | Identity | Description | |
AT4G37370.1 | 1.1e-129 | 48.32 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
AT4G37330.1 | 1.4e-124 | 47.51 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |
AT4G37320.1 | 2.5e-124 | 47.87 | cytochrome P450, family 81, subfamily D, polypeptide 5 | [more] |
AT4G37400.1 | 9.3e-124 | 48.65 | cytochrome P450, family 81, subfamily F, polypeptide 3 | [more] |
AT3G28740.1 | 1.6e-123 | 47.71 | Cytochrome P450 superfamily protein | [more] |