Sed0013258 (gene) Chayote v1

BLAST of Sed0013258 vs. NCBI nr
Match: XP_022145050.1 (probable RNA-dependent RNA polymerase 5 isoform X2 [Momordica charantia])

HSP 1 Score: 1617.1 bits (4186), Expect = 0.0e+00
Identity = 806/973 (82.84%), Postives = 864/973 (88.80%), Query Frame = 0

Query: 3    LDALSKISSRPVRSLSGFIMHLVRNDPCSTPPNKMFAVSPQRSFSSSSSSSPSTGRVSQI 62
            LD L KISSR VRSL GFI+H+VRNDPC TP  KM  VSP +        SPS+  VS  
Sbjct: 47   LDTLHKISSRAVRSLGGFILHMVRNDPC-TPQKKMVWVSPHQ--------SPSSSGVSPF 106

Query: 63   QSPSTCSDASYQSPSSCRVYSPQGLGTAERTSFQPPTPEKPQRFSYSVSDRVRIPEYVAL 122
            QSPSTCS + +QSPS+CRVYSPQGLGT ERTSFQPPT EK   FS SVSDR  IP++VAL
Sbjct: 107  QSPSTCSVSPHQSPSTCRVYSPQGLGTTERTSFQPPTLEKSGSFSSSVSDRANIPQFVAL 166

Query: 123  GELEFRKAFLILSYIGGESLESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKEDRRV 182
            GELEFRKAFLILSYIGGESLE + TADQIRSLSQ PM++FE+EVW  FG+K  +KEDRRV
Sbjct: 167  GELEFRKAFLILSYIGGESLEMVATADQIRSLSQLPMEKFELEVWKTFGEKCVSKEDRRV 226

Query: 183  YIDWDRRKTHIYHCYVSLDGRYKFKGPFLNNTKTHLQRVLGDDNVLLVKFAEDAPDRCSP 242
             +DWDRRKTHIYHCYV+LDG YKFKGPFLNNTKTHLQRVLGDDNVL+VKFA+D  DRCS 
Sbjct: 227  NVDWDRRKTHIYHCYVALDGSYKFKGPFLNNTKTHLQRVLGDDNVLMVKFAKDTSDRCST 286

Query: 243  SHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESDADS 302
            +H G  FYAYNKIARDGILLGLR+Y FFVFKDGGKEEKKKNPT SAVKCYFVRMESDA  
Sbjct: 287  NHLGGSFYAYNKIARDGILLGLRQYCFFVFKDGGKEEKKKNPTASAVKCYFVRMESDAYL 346

Query: 303  DKNEPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQRIGD 362
            DK  PYKLSN+T+FE RSLFMHAHMVSSVAS+MARFSLILSK++NLKIDLSTVNVQRIGD
Sbjct: 347  DKIRPYKLSNKTLFEARSLFMHAHMVSSVASYMARFSLILSKSINLKIDLSTVNVQRIGD 406

Query: 363  IPCRDAFGNVIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRTPIFEEFQ 422
            IPC+D +GNVIYRDGKPLIHTDGTGFISEDLALECP+NVFKGQA+H  ++KR   FEEFQ
Sbjct: 407  IPCKDVYGNVIYRDGKPLIHTDGTGFISEDLALECPMNVFKGQAKHDTNLKRFSAFEEFQ 466

Query: 423  NKTFQRTLPGLELREP-------------------LPSRTIQIRDSMIKVEIDPKLENAE 482
            NKT Q TLPGLELREP                   LPSRTIQIR+SMIKVEIDP LENA+
Sbjct: 467  NKTLQLTLPGLELREPFRLFYNGRAVKGTFLINKQLPSRTIQIRNSMIKVEIDPNLENAK 526

Query: 483  MVNSLEVVGTSNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFSSKRAALR 542
              NS+EVVGTSN PK+TFLSRNLIALLNYGGVPREYFMNIL+DAL DV+GVFSSKRAALR
Sbjct: 527  TENSMEVVGTSNHPKKTFLSRNLIALLNYGGVPREYFMNILVDALSDVQGVFSSKRAALR 586

Query: 543  VSINNGDMDDFLVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDCYYLMGTA 602
            VSINNGD+DDFLVARMILSG+PLDESYLQYRLSMLL+EEKKSLKSGRLHVP CYYLMGTA
Sbjct: 587  VSINNGDLDDFLVARMILSGIPLDESYLQYRLSMLLREEKKSLKSGRLHVPGCYYLMGTA 646

Query: 603  DPTCTLESDEVCVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYAIF 662
            DPTCTLESDEVCVILENGQ NGKVLVYRNPGLHFGDIHVLTAKYVE+LVPVVGNAKYAIF
Sbjct: 647  DPTCTLESDEVCVILENGQINGKVLVYRNPGLHFGDIHVLTAKYVEELVPVVGNAKYAIF 706

Query: 663  FSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPWRPSPSMEGITHKKPIEFSS 722
            FSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYF+PGEPWRPSPSMEGIT+KKP EFS+
Sbjct: 707  FSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEGITNKKPNEFSA 766

Query: 723  EDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAKILQLINI 782
            EDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGE+ KEERNRVRAKILQLINI
Sbjct: 767  EDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGEDRKEERNRVRAKILQLINI 826

Query: 783  YYDALDAPKKGGKKIEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVNMYQEEVPDV 842
            YYDALDAPKKGGKKIEVPKHLKA T PHF ERGKNSY STSILGQIFDTVNMYQEEVP++
Sbjct: 827  YYDALDAPKKGGKKIEVPKHLKAGTLPHFMERGKNSYTSTSILGQIFDTVNMYQEEVPNI 886

Query: 843  EVQKLPCFEDEVPDYTFMKWKFLYEMYRKDMCDALQLDPEAKNVAAEATIKKYKEILYGA 902
            EVQKLPCFED+VPD   MKWKF YEMYR +M DA+QLDP+AKNVAAEATIKKYKEILYGA
Sbjct: 887  EVQKLPCFEDQVPDDILMKWKFHYEMYRAEMKDAMQLDPDAKNVAAEATIKKYKEILYGA 946

Query: 903  EELEGSPRSIEEVYMEALAIYQVAYEYAMSHGTVSHCGFAWRVGGSALFKLCARKHSEKS 957
            EELEGSPR +EEV  EALAIY V Y++AMSHG+V +C FAWRV GSALFKLCARK SE+S
Sbjct: 947  EELEGSPRWLEEVCKEALAIYHVTYDHAMSHGSVRYCSFAWRVAGSALFKLCARKQSERS 1006

BLAST of Sed0013258 vs. NCBI nr
Match: XP_022145049.1 (probable RNA-dependent RNA polymerase 5 isoform X1 [Momordica charantia])

HSP 1 Score: 1615.1 bits (4181), Expect = 0.0e+00
Identity = 806/978 (82.41%), Postives = 864/978 (88.34%), Query Frame = 0

Query: 3    LDALSKISSRPVRSLSGFIMHLVRNDPCSTPPNKMFAVSPQRSFSSSSSSSPSTGRVSQI 62
            LD L KISSR VRSL GFI+H+VRNDPC TP  KM  VSP +        SPS+  VS  
Sbjct: 47   LDTLHKISSRAVRSLGGFILHMVRNDPC-TPQKKMVWVSPHQ--------SPSSSGVSPF 106

Query: 63   QSPSTCSDASYQSPSSCRVYSPQGLGTAERTSFQPPTPEKPQRFSYSVSDRVRIPEYVAL 122
            QSPSTCS + +QSPS+CRVYSPQGLGT ERTSFQPPT EK   FS SVSDR  IP++VAL
Sbjct: 107  QSPSTCSVSPHQSPSTCRVYSPQGLGTTERTSFQPPTLEKSGSFSSSVSDRANIPQFVAL 166

Query: 123  GELEFRKAFLILSYIGGESLESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKEDRRV 182
            GELEFRKAFLILSYIGGESLE + TADQIRSLSQ PM++FE+EVW  FG+K  +KEDRRV
Sbjct: 167  GELEFRKAFLILSYIGGESLEMVATADQIRSLSQLPMEKFELEVWKTFGEKCVSKEDRRV 226

Query: 183  YIDWDRRKTHIYHCYVSLDGRYKFKGPFLNNTKTHLQRVLGDDNVLLVKFAEDAPDRCSP 242
             +DWDRRKTHIYHCYV+LDG YKFKGPFLNNTKTHLQRVLGDDNVL+VKFA+D  DRCS 
Sbjct: 227  NVDWDRRKTHIYHCYVALDGSYKFKGPFLNNTKTHLQRVLGDDNVLMVKFAKDTSDRCST 286

Query: 243  SHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESDADS 302
            +H G  FYAYNKIARDGILLGLR+Y FFVFKDGGKEEKKKNPT SAVKCYFVRMESDA  
Sbjct: 287  NHLGGSFYAYNKIARDGILLGLRQYCFFVFKDGGKEEKKKNPTASAVKCYFVRMESDAYL 346

Query: 303  DKNEPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQRIGD 362
            DK  PYKLSN+T+FE RSLFMHAHMVSSVAS+MARFSLILSK++NLKIDLSTVNVQRIGD
Sbjct: 347  DKIRPYKLSNKTLFEARSLFMHAHMVSSVASYMARFSLILSKSINLKIDLSTVNVQRIGD 406

Query: 363  IPCRDAFGNVIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRTPIFEEFQ 422
            IPC+D +GNVIYRDGKPLIHTDGTGFISEDLALECP+NVFKGQA+H  ++KR   FEEFQ
Sbjct: 407  IPCKDVYGNVIYRDGKPLIHTDGTGFISEDLALECPMNVFKGQAKHDTNLKRFSAFEEFQ 466

Query: 423  NKTFQRTLPGLELREP------------------------LPSRTIQIRDSMIKVEIDPK 482
            NKT Q TLPGLELREP                        LPSRTIQIR+SMIKVEIDP 
Sbjct: 467  NKTLQLTLPGLELREPPLLIQFRLFYNGRAVKGTFLINKQLPSRTIQIRNSMIKVEIDPN 526

Query: 483  LENAEMVNSLEVVGTSNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFSSK 542
            LENA+  NS+EVVGTSN PK+TFLSRNLIALLNYGGVPREYFMNIL+DAL DV+GVFSSK
Sbjct: 527  LENAKTENSMEVVGTSNHPKKTFLSRNLIALLNYGGVPREYFMNILVDALSDVQGVFSSK 586

Query: 543  RAALRVSINNGDMDDFLVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDCYY 602
            RAALRVSINNGD+DDFLVARMILSG+PLDESYLQYRLSMLL+EEKKSLKSGRLHVP CYY
Sbjct: 587  RAALRVSINNGDLDDFLVARMILSGIPLDESYLQYRLSMLLREEKKSLKSGRLHVPGCYY 646

Query: 603  LMGTADPTCTLESDEVCVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNA 662
            LMGTADPTCTLESDEVCVILENGQ NGKVLVYRNPGLHFGDIHVLTAKYVE+LVPVVGNA
Sbjct: 647  LMGTADPTCTLESDEVCVILENGQINGKVLVYRNPGLHFGDIHVLTAKYVEELVPVVGNA 706

Query: 663  KYAIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPWRPSPSMEGITHKKP 722
            KYAIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYF+PGEPWRPSPSMEGIT+KKP
Sbjct: 707  KYAIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEGITNKKP 766

Query: 723  IEFSSEDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAKIL 782
             EFS+EDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGE+ KEERNRVRAKIL
Sbjct: 767  NEFSAEDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGEDRKEERNRVRAKIL 826

Query: 783  QLINIYYDALDAPKKGGKKIEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVNMYQE 842
            QLINIYYDALDAPKKGGKKIEVPKHLKA T PHF ERGKNSY STSILGQIFDTVNMYQE
Sbjct: 827  QLINIYYDALDAPKKGGKKIEVPKHLKAGTLPHFMERGKNSYTSTSILGQIFDTVNMYQE 886

Query: 843  EVPDVEVQKLPCFEDEVPDYTFMKWKFLYEMYRKDMCDALQLDPEAKNVAAEATIKKYKE 902
            EVP++EVQKLPCFED+VPD   MKWKF YEMYR +M DA+QLDP+AKNVAAEATIKKYKE
Sbjct: 887  EVPNIEVQKLPCFEDQVPDDILMKWKFHYEMYRAEMKDAMQLDPDAKNVAAEATIKKYKE 946

Query: 903  ILYGAEELEGSPRSIEEVYMEALAIYQVAYEYAMSHGTVSHCGFAWRVGGSALFKLCARK 957
            ILYGAEELEGSPR +EEV  EALAIY V Y++AMSHG+V +C FAWRV GSALFKLCARK
Sbjct: 947  ILYGAEELEGSPRWLEEVCKEALAIYHVTYDHAMSHGSVRYCSFAWRVAGSALFKLCARK 1006

BLAST of Sed0013258 vs. NCBI nr
Match: XP_038879807.1 (LOW QUALITY PROTEIN: probable RNA-dependent RNA polymerase 5 [Benincasa hispida])

HSP 1 Score: 1584.3 bits (4101), Expect = 0.0e+00
Identity = 787/977 (80.55%), Postives = 858/977 (87.82%), Query Frame = 0

Query: 3    LDALSKISSRPVRSLSGFIMHLVRNDPCSTPPNKMFAVSPQRSFSSSSSSSPSTGRVSQI 62
            L AL++IS  PV +LSGFI+ +VRNDP ++P  KM  VSP +        SPS+  VSQ+
Sbjct: 47   LHALNRISCGPVWNLSGFILDMVRNDPYTSPQKKMVHVSPHQ--------SPSSCHVSQL 106

Query: 63   QSPSTCSDASYQSPSSCRVYSPQGLGTAERTSFQPPTPEKPQRFSYSVSDRVRIPEYVAL 122
            QSP TCS ++ QSPS+C VY PQGLGTAE+ S QP  PEK + FS SV DR ++ ++VAL
Sbjct: 107  QSPPTCSVSTLQSPSTCSVYCPQGLGTAEQASIQPAMPEKSRSFSSSVLDRAKVLQFVAL 166

Query: 123  GELEFRKAFLILSYIGGESLESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKEDRRV 182
            GELEFRKAFLILSYIGGESLE +TTADQI+SLSQ  M++FE  VW  FG+KY  KEDRRV
Sbjct: 167  GELEFRKAFLILSYIGGESLERVTTADQIQSLSQLSMEKFEAVVWKTFGEKYIKKEDRRV 226

Query: 183  YIDWDRRKTHIYHCYVSLDGRYKFKGPFLNNTKTHLQRVLGDDNVLLVKFAEDAPDRCSP 242
            Y+DWDRRKTHIYHCYV+LDGRY FKGPFLN+T+THLQRVLGDDNVL+VKFAED  DR S 
Sbjct: 227  YVDWDRRKTHIYHCYVALDGRYMFKGPFLNDTRTHLQRVLGDDNVLMVKFAEDVADRYSI 286

Query: 243  SHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESDADS 302
            SH G  FYAYNKIARDGI++GLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESD   
Sbjct: 287  SHSGGSFYAYNKIARDGIVVGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESDVYI 346

Query: 303  DKNEPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQRIGD 362
            DK EPYKLSNRT+FE R+LFMHAHMVSSVAS+MARFSLILSKT+NLKIDLSTVNVQRI D
Sbjct: 347  DKIEPYKLSNRTVFEARNLFMHAHMVSSVASYMARFSLILSKTLNLKIDLSTVNVQRIRD 406

Query: 363  IPCRDAFGNVIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRTPIFEEFQ 422
            IPC+D +GNVIYRDGKPLIHTDGTGFIS DLALECP+NVFKGQA+H AD+KR P FE FQ
Sbjct: 407  IPCKDVYGNVIYRDGKPLIHTDGTGFISVDLALECPMNVFKGQAKHDADLKRIPAFEGFQ 466

Query: 423  NKTFQRTLPGLELREP------------------------LPSRTIQIRDSMIKVEIDPK 482
            NKT Q TLPGLELREP                        LPSRTIQIR+SMIKVE DP 
Sbjct: 467  NKTLQLTLPGLELREPPLLIQFRLFYNGLAVKGTFLVNKQLPSRTIQIRNSMIKVENDPD 526

Query: 483  LENAEMVNSLEVVGTSNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFSSK 542
            LENAE  NSLE+VGTSNPPKRTFLSRNLIALLNYGGVPREYFMNIL+D+L DV+GV+SSK
Sbjct: 527  LENAETENSLELVGTSNPPKRTFLSRNLIALLNYGGVPREYFMNILVDSLKDVQGVYSSK 586

Query: 543  RAALRVSINNGDMDDFLVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDCYY 602
            RAALRVSINNGDMDDFLVARMILSG+PLDESYLQYRLSMLL+EEKKSLKSGRLHVPDCYY
Sbjct: 587  RAALRVSINNGDMDDFLVARMILSGIPLDESYLQYRLSMLLREEKKSLKSGRLHVPDCYY 646

Query: 603  LMGTADPTCTLESDEVCVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNA 662
            LMGTADPTCTLES EVCVIL+NGQ NGKVLVYRNPGLHFGDIHVLTAKYVE LVPVVGNA
Sbjct: 647  LMGTADPTCTLESSEVCVILDNGQINGKVLVYRNPGLHFGDIHVLTAKYVEALVPVVGNA 706

Query: 663  KYAIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPWRPSPSMEGITHKKP 722
            KYAIFFS KGPRSVADEIAGGDFDGDMYWVSRNSQLLEYF+PGEPWRPSPSMEG+T+KKP
Sbjct: 707  KYAIFFSSKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEGVTNKKP 766

Query: 723  IEFSSEDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAKIL 782
             EFS E+LE+ELFKLFL+TRF PSYAKSVAADNWLALMDQ LMLGE+ KEERNRVRAKIL
Sbjct: 767  KEFSPEELENELFKLFLSTRFHPSYAKSVAADNWLALMDQFLMLGEDRKEERNRVRAKIL 826

Query: 783  QLINIYYDALDAPKKGGKKIEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVNMYQE 842
            QLINIYYDALDAPKK G+KIEVPKH KA T PHF ERGKNSY+STSILG IFDTVNMYQE
Sbjct: 827  QLINIYYDALDAPKKCGRKIEVPKHQKAGTLPHFMERGKNSYVSTSILGXIFDTVNMYQE 886

Query: 843  EVPDVEVQKLPCFEDEVPDYTFMKWKFLYEMYRKDMCDALQLDPEAKNVAAEATIKKYKE 902
            EVP++EVQKLPCFE+EVPDY FMKWKFLYE+YR+DM DA+ LDP+AKNVAAEATIKKYKE
Sbjct: 887  EVPNIEVQKLPCFEEEVPDYIFMKWKFLYELYRRDMKDAMHLDPDAKNVAAEATIKKYKE 946

Query: 903  ILYGAEELEGSPRSIEEVYMEALAIYQVAYEYAMSHGTVSHCGFAWRVGGSALFKLCARK 956
            ILYGAEELEGSPRSIE+VY EALAIYQV YEYAM++  VS+CGFAWRV GSALFKLCAR+
Sbjct: 947  ILYGAEELEGSPRSIEDVYQEALAIYQVTYEYAMNYNCVSYCGFAWRVAGSALFKLCARE 1006

BLAST of Sed0013258 vs. NCBI nr
Match: XP_023006718.1 (probable RNA-dependent RNA polymerase 5 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1583.2 bits (4098), Expect = 0.0e+00
Identity = 797/979 (81.41%), Postives = 858/979 (87.64%), Query Frame = 0

Query: 1    MTLDALSKISSRPVRSLSGFIMHLVRNDPCSTPPNKMFAVSPQRSFSSSSSSSPSTGRVS 60
            M LD+L KISS  +R+LSGFIMH+VRNDPC +P NKM AV     FS  SS S STG VS
Sbjct: 45   MALDSLRKISSCSIRNLSGFIMHMVRNDPCISPQNKMVAV-----FSPHSSPS-STGCVS 104

Query: 61   QIQSPSTCSDASYQSPSSCRVYSPQGLGTAERTSFQPPTPEKPQRFSYSVSDRVRIPEYV 120
            Q+ SPSTCS + YQS SSCRVYSPQ  GTAE+T F+P TP+K   +S SVSDRV+IP++V
Sbjct: 105  QLHSPSTCSASLYQSLSSCRVYSPQ--GTAEKTKFEPSTPDKSAIYSSSVSDRVQIPQFV 164

Query: 121  ALGELEFRKAFLILSYIGGESLESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKEDR 180
            ALGELEFR+AFLILSYIGGESLE +TTADQI+SLSQ  M++FE EVW  FG+KY NKEDR
Sbjct: 165  ALGELEFRRAFLILSYIGGESLERVTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDR 224

Query: 181  RVYIDWDRRKTHIYHCYVSLDGRYKFKGPFLNNTKTHLQRVLGDDNVLLVKFAEDAPDRC 240
            RVY+DWDRRKTHIYHCYV+LDG Y FKGPFLN+TKTHLQRVLGDDNVL+VKFAED  DRC
Sbjct: 225  RVYVDWDRRKTHIYHCYVALDGSYSFKGPFLNHTKTHLQRVLGDDNVLMVKFAEDVSDRC 284

Query: 241  SPSHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESDA 300
            S SHPG  FYAY KIARDGILLGLRRY+FFVFKDGGKEEKKKNPTTSAVKCYFVRMES A
Sbjct: 285  SISHPGGSFYAYKKIARDGILLGLRRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAA 344

Query: 301  DSDKNEPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQRI 360
              DK +PYKLSNRTIFE RSLFMH H VSSVAS+MARFSLILSKT+ LKIDLS+VNVQRI
Sbjct: 345  YFDKLQPYKLSNRTIFEARSLFMHPHTVSSVASYMARFSLILSKTIKLKIDLSSVNVQRI 404

Query: 361  GDIPCRDAFGNVIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRTPIFEE 420
             DIPC+D  GNV+YRDGKPLIHTDGTGFISEDLALECP+NVFKGQA+H AD+KR P FE 
Sbjct: 405  TDIPCKDVNGNVMYRDGKPLIHTDGTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEV 464

Query: 421  FQNKTFQRTLPGLELREP------------------------LPSRTIQIRDSMIKVEID 480
            FQNKT Q  LPGLELREP                        LPSRTIQIR+SMIKVE D
Sbjct: 465  FQNKTSQVILPGLELREPPLLIQFRLFYNGLAVKGTFLVNKQLPSRTIQIRNSMIKVEKD 524

Query: 481  PKLENAEMVNSLEVVGTSNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFS 540
            P LENAE  NSLEVVGTSNPPKR ++SRNLIALLNYGGVPREYFMNIL+DAL DV+G+FS
Sbjct: 525  PNLENAETENSLEVVGTSNPPKRAYISRNLIALLNYGGVPREYFMNILVDALRDVQGLFS 584

Query: 541  SKRAALRVSINNGDMDDFLVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDC 600
            SKRAALRVSINNGDMDDFLVARMILSG+PLDESYLQYRLSMLL+EEKKSL+SGRLHVPDC
Sbjct: 585  SKRAALRVSINNGDMDDFLVARMILSGIPLDESYLQYRLSMLLREEKKSLRSGRLHVPDC 644

Query: 601  YYLMGTADPTCTLESDEVCVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVG 660
            YYLMGTADPTCTLES+EVCVILENGQ NGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVG
Sbjct: 645  YYLMGTADPTCTLESNEVCVILENGQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVG 704

Query: 661  NAKYAIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPWRPSPSMEGITHK 720
            NAKY IFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYF+PG+PWRPSPSME IT+ 
Sbjct: 705  NAKYGIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFRPGKPWRPSPSMEVITNI 764

Query: 721  KPIEFSSEDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAK 780
            K  EFS+EDLE+ELFKLFLT RFQPSYAKSVAADNW+ALMDQLLMLGE+ KEERNR+RAK
Sbjct: 765  KSNEFSAEDLEEELFKLFLTARFQPSYAKSVAADNWVALMDQLLMLGEDCKEERNRLRAK 824

Query: 781  ILQLINIYYDALDAPKKGGKKIEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVNMY 840
            ILQLINIYYDALDAPKKGGK+IEVPKHLKA T PHF ERGK SY STSILGQIFDTVNM+
Sbjct: 825  ILQLINIYYDALDAPKKGGKEIEVPKHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMF 884

Query: 841  QEEVPDVEVQKLPCFEDEVPDYTFMKWKFLYEMYRKDMCDALQLDPEAKNVAAEATIKKY 900
            QEE P++EVQKLPCFEDEV D T MKWKF YEMYRK+M DALQLDP+AKNVAAEATIKKY
Sbjct: 885  QEESPNIEVQKLPCFEDEVADDTLMKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKY 944

Query: 901  KEILYGAEELEGSPRSIEEVYMEALAIYQVAYEYAMSHGTVSHCGFAWRVGGSALFKLCA 956
            KEILYGAEELEGSPR +EEVY EALAIY V YEYAMSHG V +C FAWRV GSALF LCA
Sbjct: 945  KEILYGAEELEGSPRWMEEVYKEALAIYHVTYEYAMSHGCVRYCSFAWRVAGSALFNLCA 1004

BLAST of Sed0013258 vs. NCBI nr
Match: XP_023006709.1 (probable RNA-dependent RNA polymerase 5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1578.1 bits (4085), Expect = 0.0e+00
Identity = 797/981 (81.24%), Postives = 858/981 (87.46%), Query Frame = 0

Query: 1    MTLDALSKISSRPVRSLSGFIMHLVRNDPCSTPPNKMFAVSPQRSFSSSSSSSPSTGRVS 60
            M LD+L KISS  +R+LSGFIMH+VRNDPC +P NKM AV     FS  SS S STG VS
Sbjct: 45   MALDSLRKISSCSIRNLSGFIMHMVRNDPCISPQNKMVAV-----FSPHSSPS-STGCVS 104

Query: 61   QIQSPSTCSDASYQSPSSCRVYSPQGLGTAERTSFQPPTPEKPQRFSYSVSDRVRIPEYV 120
            Q+ SPSTCS + YQS SSCRVYSPQ  GTAE+T F+P TP+K   +S SVSDRV+IP++V
Sbjct: 105  QLHSPSTCSASLYQSLSSCRVYSPQ--GTAEKTKFEPSTPDKSAIYSSSVSDRVQIPQFV 164

Query: 121  ALGELEFRKAFLILSYIGGESLESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKEDR 180
            ALGELEFR+AFLILSYIGGESLE +TTADQI+SLSQ  M++FE EVW  FG+KY NKEDR
Sbjct: 165  ALGELEFRRAFLILSYIGGESLERVTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDR 224

Query: 181  RVYIDWDRRKTHIYHCYVSLDGRYKFKGPFLNNTKTHLQRVLGDDNVLLVKFAEDAPDRC 240
            RVY+DWDRRKTHIYHCYV+LDG Y FKGPFLN+TKTHLQRVLGDDNVL+VKFAED  DRC
Sbjct: 225  RVYVDWDRRKTHIYHCYVALDGSYSFKGPFLNHTKTHLQRVLGDDNVLMVKFAEDVSDRC 284

Query: 241  SPSHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESDA 300
            S SHPG  FYAY KIARDGILLGLRRY+FFVFKDGGKEEKKKNPTTSAVKCYFVRMES A
Sbjct: 285  SISHPGGSFYAYKKIARDGILLGLRRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAA 344

Query: 301  DSDKNEPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQRI 360
              DK +PYKLSNRTIFE RSLFMH H VSSVAS+MARFSLILSKT+ LKIDLS+VNVQRI
Sbjct: 345  YFDKLQPYKLSNRTIFEARSLFMHPHTVSSVASYMARFSLILSKTIKLKIDLSSVNVQRI 404

Query: 361  GDIPCRDAFGNVIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRTPIFEE 420
             DIPC+D  GNV+YRDGKPLIHTDGTGFISEDLALECP+NVFKGQA+H AD+KR P FE 
Sbjct: 405  TDIPCKDVNGNVMYRDGKPLIHTDGTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEV 464

Query: 421  FQNKTFQRTLPGLELREP------------------------LPSRTIQIRDSMIKVEID 480
            FQNKT Q  LPGLELREP                        LPSRTIQIR+SMIKVE D
Sbjct: 465  FQNKTSQVILPGLELREPPLLIQFRLFYNGLAVKGTFLVNKQLPSRTIQIRNSMIKVEKD 524

Query: 481  PKLENAEMVNSLEVVGTSNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFS 540
            P LENAE  NSLEVVGTSNPPKR ++SRNLIALLNYGGVPREYFMNIL+DAL DV+G+FS
Sbjct: 525  PNLENAETENSLEVVGTSNPPKRAYISRNLIALLNYGGVPREYFMNILVDALRDVQGLFS 584

Query: 541  SKRAALRVSINNGDMDDFLVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDC 600
            SKRAALRVSINNGDMDDFLVARMILSG+PLDESYLQYRLSMLL+EEKKSL+SGRLHVPDC
Sbjct: 585  SKRAALRVSINNGDMDDFLVARMILSGIPLDESYLQYRLSMLLREEKKSLRSGRLHVPDC 644

Query: 601  YYLMGTADPTCTLESDEVCVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVG 660
            YYLMGTADPTCTLES+EVCVILENGQ NGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVG
Sbjct: 645  YYLMGTADPTCTLESNEVCVILENGQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVG 704

Query: 661  NAKYAIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPWRPSPSMEGITHK 720
            NAKY IFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYF+PG+PWRPSPSME IT+ 
Sbjct: 705  NAKYGIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFRPGKPWRPSPSMEVITNI 764

Query: 721  KPIEFSSEDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAK 780
            K  EFS+EDLE+ELFKLFLT RFQPSYAKSVAADNW+ALMDQLLMLGE+ KEERNR+RAK
Sbjct: 765  KSNEFSAEDLEEELFKLFLTARFQPSYAKSVAADNWVALMDQLLMLGEDCKEERNRLRAK 824

Query: 781  ILQLINIYYDALDAPKKGGKKIEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVNMY 840
            ILQLINIYYDALDAPKKGGK+IEVPKHLKA T PHF ERGK SY STSILGQIFDTVNM+
Sbjct: 825  ILQLINIYYDALDAPKKGGKEIEVPKHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMF 884

Query: 841  QEEVPDVEVQKLPCFEDEVPDYTFMKWKFLYEMYRKDMCDALQLDPEAKNVAAEATIKKY 900
            QEE P++EVQKLPCFEDEV D T MKWKF YEMYRK+M DALQLDP+AKNVAAEATIKKY
Sbjct: 885  QEESPNIEVQKLPCFEDEVADDTLMKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKY 944

Query: 901  KE--ILYGAEELEGSPRSIEEVYMEALAIYQVAYEYAMSHGTVSHCGFAWRVGGSALFKL 956
            KE  ILYGAEELEGSPR +EEVY EALAIY V YEYAMSHG V +C FAWRV GSALF L
Sbjct: 945  KELQILYGAEELEGSPRWMEEVYKEALAIYHVTYEYAMSHGCVRYCSFAWRVAGSALFNL 1004

BLAST of Sed0013258 vs. ExPASy Swiss-Prot
Match: O82188 (Probable RNA-dependent RNA polymerase 5 OS=Arabidopsis thaliana OX=3702 GN=RDR5 PE=2 SV=2)

HSP 1 Score: 831.2 bits (2146), Expect = 1.1e-239
Identity = 437/903 (48.39%), Postives = 598/903 (66.22%), Query Frame = 0

Query: 76  PSSCRVYSPQGLGTA----ERTSFQPPTPEKPQRFSYSVSDRVRIPEYVALGELEFRKAF 135
           P      SP G   +    E  S     P      S      + IP+ VALGELEF+K F
Sbjct: 84  PQPANSLSPSGRHVSRVLQEEMSVDSDAPSPKSLKSEDKGGSLHIPQLVALGELEFKKVF 143

Query: 136 LILSYIGGESLESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKEDRRVYIDWDRRKT 195
           L+LSYI G+ +  + TAD+IR     PM  +E  VW+  G+ Y  ++DRR+ + WD  KT
Sbjct: 144 LLLSYIPGQHVGQVITADEIRLWKDLPMVEYEAAVWDRLGRHYCPQKDRRM-LQWDSGKT 203

Query: 196 HIYHCYVSLDGRYKFK------GPFLNNTKTHLQRVLGDDNVLLVKFAEDAPDRCSPSHP 255
           H Y C V+ +G Y FK      GP L +T THL +VLGDDNVL VKFA+    + S ++ 
Sbjct: 204 HYYQCNVAPNGSYTFKVLSALQGPLLEHTGTHLHKVLGDDNVLTVKFAD--VQKSSSTYS 263

Query: 256 GDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESDADSDKN 315
            D ++ Y  IA++GI++GLRRY+FFVFKDGGKEEKKK+ +T  VKCYF+R +S A  D  
Sbjct: 264 IDHYFTYKGIAKNGIMIGLRRYQFFVFKDGGKEEKKKDLSTKKVKCYFIRTDSTAFYDMQ 323

Query: 316 EPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQRIGDIPC 375
            PY L+ ++I+E R  FMH H   ++A++MARFSLILSKT  L++D++ +   +I DI C
Sbjct: 324 NPYILTGKSIYEARMHFMHVHRAPTLANYMARFSLILSKTKTLEVDMTGITFDQIDDIHC 383

Query: 376 RDAFG-NVIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRT-----PIFE 435
            D  G +V+ ++ KP IH+DGTG+ISEDLA  CP+N+FKG+      I+       P+  
Sbjct: 384 HDQDGKDVLDKNKKPCIHSDGTGYISEDLARMCPLNIFKGKCLRSESIQEACYQDPPLLI 443

Query: 436 EFQNKTFQRTLPG-LELREPLPSRTIQIRDSMIKVEIDPKLENAEMVNSLEVVGTSNPPK 495
           +F+       + G   L + L  RT+Q+R SMIKV  DP L N    N+LEVV TSNPPK
Sbjct: 444 QFRMFYDGYAVKGTFLLNKKLCPRTVQVRPSMIKVSKDPSLSNFSTFNALEVVTTSNPPK 503

Query: 496 RTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFSSKRAALRVSINNGDMDDFLVAR 555
           RT LS+NL+ALL+YGG+P E+F++IL++ L + + +F +KRAAL  ++N G+MDD   A+
Sbjct: 504 RTKLSKNLVALLSYGGIPNEFFLDILLNTLEESKSIFYNKRAALNAALNYGEMDDQNAAQ 563

Query: 556 MILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDCYYLMGTADPTCTLESDEVCVIL 615
           MIL G+PLDE +L+  LS+LLK EK  LK+G+L V + YYLMGT DPT  L+ DEVCVIL
Sbjct: 564 MILVGIPLDEPHLKNYLSILLKTEKNDLKAGKLPVTESYYLMGTVDPTGALKEDEVCVIL 623

Query: 616 ENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYAIFFSCKGPRSVADEIAG 675
           E+GQ +G+VLVYRNPGLHFGDIH+L A YV+ L   VGN+K+A+FF  KGPRS+ DEIAG
Sbjct: 624 ESGQISGEVLVYRNPGLHFGDIHILKATYVKALEEYVGNSKFAVFFPQKGPRSLGDEIAG 683

Query: 676 GDFDGDMYWVSRNSQLLEYFKPGEPW-RPSPSMEGITHKKPIEFSSEDLEDELFKLFLTT 735
           GDFDGDMY++SRN +LLE FKP EPW   +P  +  + + P + S E+LE+ELF++FLT 
Sbjct: 684 GDFDGDMYFISRNPELLENFKPSEPWVSLTPPSKSNSGRAPSQLSPEELEEELFEMFLTA 743

Query: 736 RFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAKILQLINIYYDALDAPKKGGKK 795
            F  S    +AAD+WL +MD+ L+LG++  EE+  ++ K+L+LI+IYYDALDAPKK G K
Sbjct: 744 GFHASNVIGIAADSWLTIMDRFLILGDDRAEEKAEMKKKMLELIDIYYDALDAPKK-GDK 803

Query: 796 IEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVNMY--QEEVPDVEVQKLPCFEDE- 855
           + +P  LK    PH+ ER K  + STSILG IFD V     +E  P  E+ KLPCFEDE 
Sbjct: 804 VYLPNKLKPDIFPHYMERDK-KFQSTSILGLIFDFVKSQTTEEPSPSSEISKLPCFEDEP 863

Query: 856 VPDYTFMKWKFLYEMYRKDMCDALQLDPEAKNVAAEATIKKYKEILYGAEELEGSPRSIE 915
           V ++   K +  Y+ YR +M  A++ D   K+ +A   I++YK+  YGA   E S +S+E
Sbjct: 864 VSEFHMQKCRLWYDNYRTEMTQAMKTD---KDESANEVIQRYKQEFYGAAGFEDSKKSLE 923

Query: 916 EVYMEALAIYQVAYEYAMSHGTVSHCGFAWRVGGSALFKL-CARKHSEKSFLCLPSVMRE 957
           E+Y +ALA+Y++ Y+YA+ H  VS C F W+V G  L +    +K  EK  +C PSV++E
Sbjct: 924 ELYPQALALYKIVYDYAI-HAGVSKCRFVWKVAGPVLCRFYLNKKMQEKCLVCAPSVLKE 977

BLAST of Sed0013258 vs. ExPASy Swiss-Prot
Match: O82190 (Probable RNA-dependent RNA polymerase 3 OS=Arabidopsis thaliana OX=3702 GN=RDR3 PE=3 SV=2)

HSP 1 Score: 801.6 bits (2069), Expect = 9.7e-231
Identity = 453/979 (46.27%), Postives = 612/979 (62.51%), Query Frame = 0

Query: 1   MTLDALSKISSRP-VRSLSGFIMHLVRNDPCSTPPNKMFAVSPQRSFSSSSSSSPSTGRV 60
           + L+ L K+ ++P +++L G IM+ V+             V+   S   S   SP     
Sbjct: 53  LALETLRKVFNKPYLKTLDGLIMYFVKG-----------TVTVDGSPRLSPGESP----- 112

Query: 61  SQIQSPSTCSDASYQSPSSCRVYSPQGLGTAERTSFQPPTPEKPQRFSYSVSDRVRIPEY 120
             +QSP T       +  SCR      L        + P+P+  +R     S    IP  
Sbjct: 113 --VQSPRT------PAKKSCRASQDVSL------DLETPSPKFMKREENGGSK--YIPPL 172

Query: 121 VALGELEFRKAFLILSYIGGESL-ESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKE 180
           +ALGELEF+KAFL+LSYIGGESL E + + DQIR     PM  +E  VWN  G++Y + +
Sbjct: 173 LALGELEFKKAFLLLSYIGGESLVEEVISGDQIRKWKDLPMVSYEAAVWNRLGQRYCSPK 232

Query: 181 DRRVYIDWDRRKTHIYHCYVSLDGRYKFKGPFLNNTKTHLQRVLGDDNVLLVKFAEDAPD 240
           +RR  ++ D   TH Y C+V+ DG YKFKG  L NT THL +VLGDDNVL VKF +    
Sbjct: 233 ERRRPLEGDSGMTHYYQCHVATDGSYKFKGHLLENTGTHLHKVLGDDNVLTVKFDKVLG- 292

Query: 241 RCSPSHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMES 300
               ++  D +  Y  IA++GI++GLRRYRFFVFKDGGKEEKKK+ +T  VKCYF+R +S
Sbjct: 293 --VETYCNDLYSTYKGIAKNGIMVGLRRYRFFVFKDGGKEEKKKDVSTKGVKCYFIRTDS 352

Query: 301 DADSDKNEPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQ 360
            A  D   PY  + +++ E R  FMH + +SS+ ++M+RFSLILSKT  L++D++ +  +
Sbjct: 353 TASIDMQNPYIFAGKSMHEARMHFMHVNTLSSLPNYMSRFSLILSKTKTLEVDMTGITFE 412

Query: 361 RIGDIPCRDAFG-NVIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRTPI 420
           +I DI C D    +V+ ++GKP IH+DGTG+ISEDLA  CP+N+FKG++    +I+   +
Sbjct: 413 QIDDIHCHDQDDKDVLDKNGKPCIHSDGTGYISEDLARMCPVNIFKGKSMRSNNIQSKNL 472

Query: 421 FEEFQNKTFQRTLPGLELR---------------EPLPSRTIQIRDSMIKVEIDPKLENA 480
             E Q    Q     ++ R               + LP RT+Q+R SMIKV  D  L N 
Sbjct: 473 NFEGQGPCGQEPPLLIQFRIFYNGYAVKGTFLTNKKLPPRTVQVRPSMIKVYEDRTLSNL 532

Query: 481 EMVNSLEVVGTSNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFSSKRAAL 540
              NSLEVV TSNPP++  LSRNL+ALL+YGGVP ++F+NIL + L + + +F S+RAA 
Sbjct: 533 STFNSLEVVTTSNPPRKARLSRNLVALLSYGGVPNDFFLNILRNTLEESKTIFYSERAAF 592

Query: 541 RVSINNGDMDDFLVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDCYYLMGT 600
           + +IN G  DD   A MIL G+PLDE YL+ RLS LLK E+ +LK+GR  + + YY+MGT
Sbjct: 593 KAAINYG--DDQYTADMILVGIPLDEPYLKDRLSYLLKTERNALKAGRFPIDESYYIMGT 652

Query: 601 ADPTCTLESDEVCVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYAI 660
            DPT  L+ +E+CVIL +GQ +G VLVYRNPGLHFGDIHVL A YV+ L   VGNAK+A+
Sbjct: 653 VDPTGELKENEICVILHSGQISGDVLVYRNPGLHFGDIHVLKATYVKALEDYVGNAKFAV 712

Query: 661 FFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPWRPSPSMEGI-THKKPIEF 720
           FF  KGPRS+ DEIAGGDFDGDMY++SRN +LLE+FKP EPW  S     I   +KP E 
Sbjct: 713 FFPQKGPRSLGDEIAGGDFDGDMYFISRNPKLLEHFKPSEPWVSSSKPSKIYCGRKPSEL 772

Query: 721 SSEDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAKILQLI 780
           S E+LE+ELFK+FL  RF       +AAD WL +MD  L LG+   +E+   +  IL+LI
Sbjct: 773 SEEELEEELFKMFLKARFCKRDVIGMAADCWLGIMDPFLTLGDESAKEKYERKKNILKLI 832

Query: 781 NIYYDALDAPKKGGKKIEVPKHLKAVTSPHFFERG-KNSYISTSILGQIFDTVNMYQ-EE 840
           +IYYDALDAPKKG  K+++P  L+    PH+ ER  K  + STSILG IFDTV+ +  EE
Sbjct: 833 DIYYDALDAPKKGA-KVDLPPDLEIKNFPHYMERDPKRDFRSTSILGLIFDTVDSHNAEE 892

Query: 841 VPDVEVQKLPCFEDE-VPDYTFMKWKFLYEMYRKDMCDA-LQLDPEAKNVAAEATIKKYK 900
            P  E+ KL  FEDE VP     K+   YE YR +M  A ++ D   +N      I++YK
Sbjct: 893 PPPSEISKLWYFEDEPVPKSHMDKFTSWYENYRSEMSQAMMETDKVKRNQLTNEVIQRYK 952

Query: 901 EILYGAEELEGSPRSIEEVYMEALAIYQVAYEYAMSHGTVSHCGFAWRVGGSALFKLCAR 957
           +  YGA   E S +S+EE+Y +ALA+Y V Y+YA+  G V+ C FAW V G  L K   +
Sbjct: 953 QDFYGAAGFEDSNKSLEELYPQALALYNVVYDYAIQEG-VAKCTFAWNVAGPVLCKFYLK 992

BLAST of Sed0013258 vs. ExPASy Swiss-Prot
Match: O82189 (Probable RNA-dependent RNA polymerase 4 OS=Arabidopsis thaliana OX=3702 GN=RDR4 PE=3 SV=2)

HSP 1 Score: 729.9 bits (1883), Expect = 3.6e-209
Identity = 385/797 (48.31%), Postives = 532/797 (66.75%), Query Frame = 0

Query: 120 VALGELEFRKAFLILSYIGGESLESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKED 179
           +ALGELEF+KAFL+L+Y+GG+SL  + + D+IR     PM  +E  VW   G     + +
Sbjct: 147 LALGELEFKKAFLLLTYLGGKSLGEVISGDEIRQWKDLPMVAYERAVWFKLG-----QNE 206

Query: 180 RRVYIDWDRRKTHIYHCYVSLDGRYKFKGPFLNNTKTHLQRVLGDDNVLLVKFAEDAPDR 239
            R+ ++ D  KTH Y C+V+ DG Y+ KG FL NT THL +VLGDDNVL V+F +   + 
Sbjct: 207 ERMQLESDSGKTHYYQCHVAPDGSYRLKGYFLENTGTHLHKVLGDDNVLTVRFDQLPKES 266

Query: 240 CSPSHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESD 299
               +P   +  Y +IA++GI++GLRRY+FFVFKDGGK EKKK  +T  VKCYF+R  S 
Sbjct: 267 TYCDNP---YSKYKEIAKNGIMVGLRRYQFFVFKDGGKAEKKKRNSTKQVKCYFIRTGST 326

Query: 300 ADSDKNEPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQR 359
           A SD   PY LS  +I E R  FMH H + S A++MARFSLILSKT  L++D++ + V +
Sbjct: 327 ASSDMENPYILSGMSIHEARMHFMHVHTLPSPANYMARFSLILSKTKKLEVDMTEITVMQ 386

Query: 360 IGDIPCRDAFGN-VIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRT--- 419
           I DI C D   N V+ ++GKP IH+DGTG+ISEDLA  CP+N+FKG++    +I+ T   
Sbjct: 387 IDDIHCHDQSNNDVLDKNGKPRIHSDGTGYISEDLARMCPLNIFKGKSMRSNNIQGTCVQ 446

Query: 420 --PIFEEFQNKTFQRTLPGL-ELREPLPSRTIQIRDSMIKVEIDPKLENAEMVNSLEVVG 479
             P+  + +       + G+  L + LP +T+Q+R SMIKV  D  L N    NSLEVV 
Sbjct: 447 EPPLLIQIRMFNDGSAVKGIFLLNKNLPPQTVQVRPSMIKVYKDKNLSNFSTFNSLEVVT 506

Query: 480 TSNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFSSKRAALRVSINNGDMD 539
           TSNPPKR  LS+NL+ALL+YGGVP ++F++IL++ L   + +F   RAA + +++ G+MD
Sbjct: 507 TSNPPKRAKLSKNLVALLSYGGVPNDFFLDILLNTLEKKKTIFFKVRAAGKAALHYGNMD 566

Query: 540 DFLVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDCYYLMGTADPTCTLESD 599
           D    +MI++G+PLDE YL++ LS LLK EK  LK+G+L + + YYLMGT DPT  L+ D
Sbjct: 567 DKNALQMIMAGIPLDEPYLKHYLSKLLKLEKDDLKAGKLPIDESYYLMGTVDPTGELKED 626

Query: 600 EVCVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYAIFFSCKGPRSV 659
           EV  + ++      VLVYRNPGLHFGDIH+L A YV+ L   VGN+KY +FF  KGPRS+
Sbjct: 627 EVSGLAKSQD----VLVYRNPGLHFGDIHILKATYVKSLEQYVGNSKYGVFFPQKGPRSL 686

Query: 660 ADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPW-RPSPSMEGITHKKPIEFSSEDLEDELF 719
            DEIAGGDFDGDMY++SRN +LLE++KP EPW   SP  +  T ++P E S E LE+ELF
Sbjct: 687 GDEIAGGDFDGDMYFISRNPKLLEHYKPSEPWVSSSPRSKIYTGRQPSELSPEQLEEELF 746

Query: 720 KLFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAKILQLINIYYDALDAP 779
           K+FL T F PS     AAD+WLA+MD+ L LG+ + +E+  ++ K+L+L +IYYDA+DAP
Sbjct: 747 KIFLKTGFSPSSVIGQAADSWLAIMDRFLTLGDENVKEKAEMKKKMLKLTDIYYDAIDAP 806

Query: 780 KKGGKKIEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVNMYQ-EEVPDVEVQKLPC 839
           K  G ++ +P  +K    PH+ ER K ++ STSILG IFDTV+ +  E+     + KL C
Sbjct: 807 KT-GTEVNLPLDVKVDLFPHYMERNK-TFKSTSILGLIFDTVDFHNAEDTTPSGISKLQC 866

Query: 840 FEDE-VPDYTFMKWKFLYEMYRKDMCDALQLDPEAKNVAAEATIKKYKEILYGAEELEGS 899
           FEDE V ++   K K  ++ YRK+MC A+  D +    +    I+KYK+  Y A   + S
Sbjct: 867 FEDEPVSEFDMEKCKLWHKDYRKEMCQAMNSDDDD---SCNEVIQKYKQEFYSAAGFKES 926

Query: 900 PRSIEEVYMEALAIYQV 907
            + +EE+Y +ALA+Y V
Sbjct: 927 KKILEELYPKALALYNV 926

BLAST of Sed0013258 vs. ExPASy Swiss-Prot
Match: Q5QMN5 (Probable RNA-dependent RNA polymerase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR3 PE=2 SV=2)

HSP 1 Score: 723.0 bits (1865), Expect = 4.4e-207
Identity = 418/967 (43.23%), Postives = 559/967 (57.81%), Query Frame = 0

Query: 29   PCSTPPNKMFAVSPQRSFSSSSSSSPSTGRVSQIQSPSTCSDASYQSPSSCRVYSPQGLG 88
            PC   P+ +     Q      S   P      ++ +PS+  D S       R+       
Sbjct: 211  PCRCMPSGL-----QNYIEIDSPIQPMISTPRRVSTPSSVQDIS-------RLIENMAGP 270

Query: 89   TAERTSFQPPTPEKPQRFSYSVSDRVR---IPEYVALGELEFRKAFLILSYIGGESLESI 148
            +    S     P+ P    ++  + +R    P+ +AL EL FRK F++ +Y+  E +E++
Sbjct: 271  SVSPPSPITAMPQNPTTTCHTTDNALREAASPQMLALEELGFRKIFMVFAYLASEKIENV 330

Query: 149  TTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKEDRRVYIDWDRRKTHIYHCYVSLDGRYK 208
             + D IRSL    M +FE ++W  FG KY    DR   +D D   T +YHC V++ G   
Sbjct: 331  LSVDYIRSLKFLSMAQFESQIWRTFGHKYIAASDRAKNLDSDPGMTKVYHCNVAIRGDTV 390

Query: 209  ---FKGPFLNNTKTHLQRVLGDDNVLLVKFAEDAPDRCSPSHPGDFFYAYNKIARDGILL 268
               FKGP++ NT+THLQ+V+GDDNVL+VKF     D  + +    +   Y+K+A DGI+L
Sbjct: 391  VKIFKGPYIENTRTHLQKVVGDDNVLVVKFMGKLSD--TKTDFSTYCEHYHKVAEDGIVL 450

Query: 269  GLRRYRFFVFKDGGKEEK-------KKNPTTSAVKCYFVRMESDADSDKNEPYKLSNRTI 328
            GLRRYRFFV+KDGGKEEK        KN  TS V CYFVR ES  + D  EPY LS RT+
Sbjct: 451  GLRRYRFFVYKDGGKEEKLKQEKIEDKNKCTSPVMCYFVRTESGWNMD--EPYILSGRTV 510

Query: 329  FEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQRIGDIPCRDAFGNVIYR 388
             + R LFMH     ++A +MARF+LILSKT+    DLS V V+RI D PC D  GNV+++
Sbjct: 511  GQARELFMHISSAPTLAKYMARFALILSKTITWDADLSAVYVRRIKDEPCMDRHGNVVHK 570

Query: 389  DGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRTPIFEEFQNKTFQRTLPGLEL 448
            D +PLIHTDGTG +S DLAL CP ++FKG+      +K  P+  +F+       + G  L
Sbjct: 571  DQEPLIHTDGTGLVSVDLALNCPTSIFKGKF-----LKPQPLLMQFRLFYNGSAVKGTVL 630

Query: 449  RE-PLPSRTIQIRDSMIKVEIDPK---------------------LENAEMVNSLEVVGT 508
             +  LP  TI IR SM+K+E  P+                     L   + VNS E+V T
Sbjct: 631  VDRRLPPATILIRPSMVKIETHPELSGVRSVNSSEIVSARNAKKSLSGVQSVNSFEIVST 690

Query: 509  SNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFSSKRAALRVSINNGDMDD 568
            SN P+RT  SR LI LL YGGVP EYF+ +L  A+            ALR++ +  DM+D
Sbjct: 691  SNRPRRTLTSRFLITLLCYGGVPEEYFLELLQSAIEGAENACYDYEDALRIAFSYADMED 750

Query: 569  FLVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDCYYLMGTADPTCTLESDE 628
             + ARMILSG+PL+ESYLQ+RL  + ++E+K +K G++ + +CYYLM T DPT TL  +E
Sbjct: 751  SMSARMILSGIPLEESYLQHRLDFMAQQERKGIKQGKIPIDECYYLMDTTDPTGTLRPNE 810

Query: 629  VCVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDL-VPVVGNAKYAIFFSCKGPRSV 688
            VCVILENGQF+G VLVY++PGLHFGDIHVL A Y+ DL    VG AKYAI F   GPRS+
Sbjct: 811  VCVILENGQFSGDVLVYKHPGLHFGDIHVLKATYIRDLEKEYVGYAKYAILFPISGPRSL 870

Query: 689  ADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPWRPSPSMEGITHKKPIEFSSEDLEDELFK 748
            ADE+A  DFDGD+YWVS+N +LLE+FKP EPW  +   +    KKP + +   LE  LF 
Sbjct: 871  ADEMANSDFDGDIYWVSKNPKLLEHFKPSEPWVQAIKPKKTKQKKPQDCNESKLERLLFH 930

Query: 749  LFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAKILQLINIYYDALDAPK 808
             FL TRF PS+A   AAD+WLA MD+LL      + E+  +  K+L+L+++YY ALDAPK
Sbjct: 931  EFLKTRFTPSFALGTAADSWLAYMDRLL-TDSLDEIEKKLIEEKMLKLVDLYYLALDAPK 990

Query: 809  KGGKKIEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVNMYQE----EVPDVEVQKL 868
              G K+ +P  L     PHF  R   SY S+SILGQI+D     +      V  + V  L
Sbjct: 991  T-GNKVNIPSDLMVKQYPHFMGR-SFSYHSSSILGQIYDKAEDVESLRSCNVQPIGVSLL 1050

Query: 869  PCF-EDEVPDYTFMKWKFLYEMYRKD--MCDALQLDPEAKNVAAEATIKKYKEILYGAEE 928
            PCF E E P      W+  YE Y  D  M     +D E +N+  +   +KYK +LY A E
Sbjct: 1051 PCFMEREAPPAARHLWQHRYEEYLTDSTMLYRAMVDKEERNMKFQELYEKYKHMLYDASE 1110

Query: 929  LEGSPRSIEEVYMEALAIYQVAYEYAMSHGTVSHCGFAWRVGGSALFKLCARKHSEKSFL 953
             E + R  ++V+ EA  IYQ+ YE A      S CGFAW+V G AL    A K+   + L
Sbjct: 1111 FEQTQRDPDDVFSEACVIYQIVYEKARWSNDASRCGFAWKVAGRALCHFYALKNEGDTAL 1153

BLAST of Sed0013258 vs. ExPASy Swiss-Prot
Match: Q5QMN4 (Probable RNA-dependent RNA polymerase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR4 PE=2 SV=2)

HSP 1 Score: 707.2 bits (1824), Expect = 2.5e-202
Identity = 397/905 (43.87%), Postives = 536/905 (59.23%), Query Frame = 0

Query: 117  PEYVALGELEFRKAFLILSYIG--------------GESLESITTADQIRSLSQFPMDRF 176
            P+ +ALGELEFRK F+I +Y+                  +E + + D IRSL    M +F
Sbjct: 277  PQMLALGELEFRKIFMIFAYLSWNKKGVKPPLSTPKSSKIEDVLSVDSIRSLKSMSMAQF 336

Query: 177  EVEVWNLFGKKYTNKEDRRVYIDWDRRKTHIYHCYVSLDGRYK---FKGPFLNNTKTHLQ 236
            E  +W+ FG    +  DR   +D     + +YHC V + G      FKGP++ N +THLQ
Sbjct: 337  ESRIWSTFGHDNISSSDRAKNLDSGPGMSKVYHCNVEIRGGTVVKIFKGPYIENRRTHLQ 396

Query: 237  RVLGDDNVLLVKFAEDAPDRCSPSHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEE 296
            +VLGDDNVL+VKF E + D  + +    +   Y+K+A +GI+LGLR YRFF++KDGGKE 
Sbjct: 397  KVLGDDNVLVVKFMEISSD--TETDLSTYLEHYHKVAEEGIVLGLRCYRFFLYKDGGKEN 456

Query: 297  KKKNPT--------TSAVKCYFVRMESDADSDKNEPYKLSNRTIFEGRSLFMHAHMVSSV 356
            K K           TS+V+CYFVR ES  + D  EPY LS RTI + R LFMH   V ++
Sbjct: 457  KMKEENREEENKKCTSSVRCYFVRTESGWNMD--EPYILSGRTIGQARDLFMHIRTVLTL 516

Query: 357  ASFMARFSLILSKTVNLKIDLSTVNVQRIGDIPCRDAFGNVIYRDGKPLIHTDGTGFISE 416
            A +M RF+LI+SKT+ L +DLS V V+ I D PC D  G  ++RDG+ LIHTDGTG ISE
Sbjct: 517  AKYMLRFALIVSKTITLDVDLSEVLVKLIDDEPCLDEHGKEVFRDGERLIHTDGTGLISE 576

Query: 417  DLALECPINVFKGQARHGADIKRTPIFEEF-QNKTFQRTLPGLELR-------------- 476
            DLA +C  +  KG+     DI      +    N T +  L  ++LR              
Sbjct: 577  DLAQKCTYSNSKGKLLEPQDIVDCAASKLMGSNNTTEYPLL-IQLRLFYNGSAVKGTVLV 636

Query: 477  -EPLPSRTIQIRDSMIKVEIDPKLENAEMVNSL---------------------EVVGTS 536
             + LP RTI IR SM+K++  P+L   + VNSL                     E+V TS
Sbjct: 637  DKRLPPRTIHIRPSMLKIKTYPELSGVQSVNSLDIVSARNAKKSLSGVQSVNSFEIVSTS 696

Query: 537  NPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFSSKRAALRVSINNGDMDDF 596
            N   RTF S NLIALL+YGGVP E+FM +L  A+ +          AL ++ N  DM+D 
Sbjct: 697  NRSGRTFTSNNLIALLHYGGVPEEFFMELLQTAIEEADNARFDYAGALNIAFNYADMEDS 756

Query: 597  LVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDCYYLMGTADPTCTLESDEV 656
            + ARMILSG+PL+ESYLQ RL  L   E+K +K+G++ + DCYYLMGTADPT  L  +EV
Sbjct: 757  MPARMILSGIPLEESYLQSRLDFLSLLERKGIKNGKIPIDDCYYLMGTADPTGKLGPNEV 816

Query: 657  CVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYAIFFSCKGPRSVAD 716
            CVIL+ GQ +G VLVY+ PGLH GDIHVL A Y  D+  VVGN+K+AI F   G RS+AD
Sbjct: 817  CVILDYGQVSGDVLVYKYPGLHPGDIHVLKATYSSDIEKVVGNSKHAILFPTTGQRSLAD 876

Query: 717  EIAGGDFDGDMYWVSRNSQLLEYFKPGEPWRPSPSMEGITHKKPIEFSSEDLEDELFKLF 776
            E+A  DFDGD+YWVS N +LLE+FKP +PW P+ +  G   K P +F+  +LE  LF  F
Sbjct: 877  EMANSDFDGDIYWVSLNPKLLEHFKPSKPWVPAITPNGTKQKGPEDFNESELERVLFHEF 936

Query: 777  LTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAKILQLINIYYDALDAPKKG 836
            L TRF PSYA++ AA NWL  MD+LL +  + + E+  +  K+L+L+++YY ALDAPK  
Sbjct: 937  LKTRFAPSYARATAATNWLVYMDRLLTVSLD-ESEKKLIEKKMLKLVDLYYLALDAPKM- 996

Query: 837  GKKIEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVN----MYQEEVPDVEVQKLPC 896
            G K+ +P+ L     PHF +R   SY S+SILG+I+D       +  + V    +  LPC
Sbjct: 997  GNKVNIPRDLMVKQYPHFMDRSP-SYHSSSILGKIYDKAGDPKPLRSDNVQPTSISSLPC 1056

Query: 897  F-EDEVPDYTFMKWKFLYEMYRKD--MCDALQLDPEAKNVAAEATIKKYKEILYGAEELE 953
            F E +VP      W+  Y  Y  D  +  A + D E K +  +   +KYK +LYGA E E
Sbjct: 1057 FAERDVPPAIKQLWQHRYNEYLADSSLLYAEEADEEEKKIKFQELYEKYKHLLYGASEFE 1116

BLAST of Sed0013258 vs. ExPASy TrEMBL
Match: A0A6J1CTD3 (RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111014566 PE=3 SV=1)

HSP 1 Score: 1617.1 bits (4186), Expect = 0.0e+00
Identity = 806/973 (82.84%), Postives = 864/973 (88.80%), Query Frame = 0

Query: 3    LDALSKISSRPVRSLSGFIMHLVRNDPCSTPPNKMFAVSPQRSFSSSSSSSPSTGRVSQI 62
            LD L KISSR VRSL GFI+H+VRNDPC TP  KM  VSP +        SPS+  VS  
Sbjct: 47   LDTLHKISSRAVRSLGGFILHMVRNDPC-TPQKKMVWVSPHQ--------SPSSSGVSPF 106

Query: 63   QSPSTCSDASYQSPSSCRVYSPQGLGTAERTSFQPPTPEKPQRFSYSVSDRVRIPEYVAL 122
            QSPSTCS + +QSPS+CRVYSPQGLGT ERTSFQPPT EK   FS SVSDR  IP++VAL
Sbjct: 107  QSPSTCSVSPHQSPSTCRVYSPQGLGTTERTSFQPPTLEKSGSFSSSVSDRANIPQFVAL 166

Query: 123  GELEFRKAFLILSYIGGESLESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKEDRRV 182
            GELEFRKAFLILSYIGGESLE + TADQIRSLSQ PM++FE+EVW  FG+K  +KEDRRV
Sbjct: 167  GELEFRKAFLILSYIGGESLEMVATADQIRSLSQLPMEKFELEVWKTFGEKCVSKEDRRV 226

Query: 183  YIDWDRRKTHIYHCYVSLDGRYKFKGPFLNNTKTHLQRVLGDDNVLLVKFAEDAPDRCSP 242
             +DWDRRKTHIYHCYV+LDG YKFKGPFLNNTKTHLQRVLGDDNVL+VKFA+D  DRCS 
Sbjct: 227  NVDWDRRKTHIYHCYVALDGSYKFKGPFLNNTKTHLQRVLGDDNVLMVKFAKDTSDRCST 286

Query: 243  SHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESDADS 302
            +H G  FYAYNKIARDGILLGLR+Y FFVFKDGGKEEKKKNPT SAVKCYFVRMESDA  
Sbjct: 287  NHLGGSFYAYNKIARDGILLGLRQYCFFVFKDGGKEEKKKNPTASAVKCYFVRMESDAYL 346

Query: 303  DKNEPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQRIGD 362
            DK  PYKLSN+T+FE RSLFMHAHMVSSVAS+MARFSLILSK++NLKIDLSTVNVQRIGD
Sbjct: 347  DKIRPYKLSNKTLFEARSLFMHAHMVSSVASYMARFSLILSKSINLKIDLSTVNVQRIGD 406

Query: 363  IPCRDAFGNVIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRTPIFEEFQ 422
            IPC+D +GNVIYRDGKPLIHTDGTGFISEDLALECP+NVFKGQA+H  ++KR   FEEFQ
Sbjct: 407  IPCKDVYGNVIYRDGKPLIHTDGTGFISEDLALECPMNVFKGQAKHDTNLKRFSAFEEFQ 466

Query: 423  NKTFQRTLPGLELREP-------------------LPSRTIQIRDSMIKVEIDPKLENAE 482
            NKT Q TLPGLELREP                   LPSRTIQIR+SMIKVEIDP LENA+
Sbjct: 467  NKTLQLTLPGLELREPFRLFYNGRAVKGTFLINKQLPSRTIQIRNSMIKVEIDPNLENAK 526

Query: 483  MVNSLEVVGTSNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFSSKRAALR 542
              NS+EVVGTSN PK+TFLSRNLIALLNYGGVPREYFMNIL+DAL DV+GVFSSKRAALR
Sbjct: 527  TENSMEVVGTSNHPKKTFLSRNLIALLNYGGVPREYFMNILVDALSDVQGVFSSKRAALR 586

Query: 543  VSINNGDMDDFLVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDCYYLMGTA 602
            VSINNGD+DDFLVARMILSG+PLDESYLQYRLSMLL+EEKKSLKSGRLHVP CYYLMGTA
Sbjct: 587  VSINNGDLDDFLVARMILSGIPLDESYLQYRLSMLLREEKKSLKSGRLHVPGCYYLMGTA 646

Query: 603  DPTCTLESDEVCVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYAIF 662
            DPTCTLESDEVCVILENGQ NGKVLVYRNPGLHFGDIHVLTAKYVE+LVPVVGNAKYAIF
Sbjct: 647  DPTCTLESDEVCVILENGQINGKVLVYRNPGLHFGDIHVLTAKYVEELVPVVGNAKYAIF 706

Query: 663  FSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPWRPSPSMEGITHKKPIEFSS 722
            FSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYF+PGEPWRPSPSMEGIT+KKP EFS+
Sbjct: 707  FSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEGITNKKPNEFSA 766

Query: 723  EDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAKILQLINI 782
            EDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGE+ KEERNRVRAKILQLINI
Sbjct: 767  EDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGEDRKEERNRVRAKILQLINI 826

Query: 783  YYDALDAPKKGGKKIEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVNMYQEEVPDV 842
            YYDALDAPKKGGKKIEVPKHLKA T PHF ERGKNSY STSILGQIFDTVNMYQEEVP++
Sbjct: 827  YYDALDAPKKGGKKIEVPKHLKAGTLPHFMERGKNSYTSTSILGQIFDTVNMYQEEVPNI 886

Query: 843  EVQKLPCFEDEVPDYTFMKWKFLYEMYRKDMCDALQLDPEAKNVAAEATIKKYKEILYGA 902
            EVQKLPCFED+VPD   MKWKF YEMYR +M DA+QLDP+AKNVAAEATIKKYKEILYGA
Sbjct: 887  EVQKLPCFEDQVPDDILMKWKFHYEMYRAEMKDAMQLDPDAKNVAAEATIKKYKEILYGA 946

Query: 903  EELEGSPRSIEEVYMEALAIYQVAYEYAMSHGTVSHCGFAWRVGGSALFKLCARKHSEKS 957
            EELEGSPR +EEV  EALAIY V Y++AMSHG+V +C FAWRV GSALFKLCARK SE+S
Sbjct: 947  EELEGSPRWLEEVCKEALAIYHVTYDHAMSHGSVRYCSFAWRVAGSALFKLCARKQSERS 1006

BLAST of Sed0013258 vs. ExPASy TrEMBL
Match: A0A6J1CU26 (RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111014566 PE=3 SV=1)

HSP 1 Score: 1615.1 bits (4181), Expect = 0.0e+00
Identity = 806/978 (82.41%), Postives = 864/978 (88.34%), Query Frame = 0

Query: 3    LDALSKISSRPVRSLSGFIMHLVRNDPCSTPPNKMFAVSPQRSFSSSSSSSPSTGRVSQI 62
            LD L KISSR VRSL GFI+H+VRNDPC TP  KM  VSP +        SPS+  VS  
Sbjct: 47   LDTLHKISSRAVRSLGGFILHMVRNDPC-TPQKKMVWVSPHQ--------SPSSSGVSPF 106

Query: 63   QSPSTCSDASYQSPSSCRVYSPQGLGTAERTSFQPPTPEKPQRFSYSVSDRVRIPEYVAL 122
            QSPSTCS + +QSPS+CRVYSPQGLGT ERTSFQPPT EK   FS SVSDR  IP++VAL
Sbjct: 107  QSPSTCSVSPHQSPSTCRVYSPQGLGTTERTSFQPPTLEKSGSFSSSVSDRANIPQFVAL 166

Query: 123  GELEFRKAFLILSYIGGESLESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKEDRRV 182
            GELEFRKAFLILSYIGGESLE + TADQIRSLSQ PM++FE+EVW  FG+K  +KEDRRV
Sbjct: 167  GELEFRKAFLILSYIGGESLEMVATADQIRSLSQLPMEKFELEVWKTFGEKCVSKEDRRV 226

Query: 183  YIDWDRRKTHIYHCYVSLDGRYKFKGPFLNNTKTHLQRVLGDDNVLLVKFAEDAPDRCSP 242
             +DWDRRKTHIYHCYV+LDG YKFKGPFLNNTKTHLQRVLGDDNVL+VKFA+D  DRCS 
Sbjct: 227  NVDWDRRKTHIYHCYVALDGSYKFKGPFLNNTKTHLQRVLGDDNVLMVKFAKDTSDRCST 286

Query: 243  SHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESDADS 302
            +H G  FYAYNKIARDGILLGLR+Y FFVFKDGGKEEKKKNPT SAVKCYFVRMESDA  
Sbjct: 287  NHLGGSFYAYNKIARDGILLGLRQYCFFVFKDGGKEEKKKNPTASAVKCYFVRMESDAYL 346

Query: 303  DKNEPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQRIGD 362
            DK  PYKLSN+T+FE RSLFMHAHMVSSVAS+MARFSLILSK++NLKIDLSTVNVQRIGD
Sbjct: 347  DKIRPYKLSNKTLFEARSLFMHAHMVSSVASYMARFSLILSKSINLKIDLSTVNVQRIGD 406

Query: 363  IPCRDAFGNVIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRTPIFEEFQ 422
            IPC+D +GNVIYRDGKPLIHTDGTGFISEDLALECP+NVFKGQA+H  ++KR   FEEFQ
Sbjct: 407  IPCKDVYGNVIYRDGKPLIHTDGTGFISEDLALECPMNVFKGQAKHDTNLKRFSAFEEFQ 466

Query: 423  NKTFQRTLPGLELREP------------------------LPSRTIQIRDSMIKVEIDPK 482
            NKT Q TLPGLELREP                        LPSRTIQIR+SMIKVEIDP 
Sbjct: 467  NKTLQLTLPGLELREPPLLIQFRLFYNGRAVKGTFLINKQLPSRTIQIRNSMIKVEIDPN 526

Query: 483  LENAEMVNSLEVVGTSNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFSSK 542
            LENA+  NS+EVVGTSN PK+TFLSRNLIALLNYGGVPREYFMNIL+DAL DV+GVFSSK
Sbjct: 527  LENAKTENSMEVVGTSNHPKKTFLSRNLIALLNYGGVPREYFMNILVDALSDVQGVFSSK 586

Query: 543  RAALRVSINNGDMDDFLVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDCYY 602
            RAALRVSINNGD+DDFLVARMILSG+PLDESYLQYRLSMLL+EEKKSLKSGRLHVP CYY
Sbjct: 587  RAALRVSINNGDLDDFLVARMILSGIPLDESYLQYRLSMLLREEKKSLKSGRLHVPGCYY 646

Query: 603  LMGTADPTCTLESDEVCVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNA 662
            LMGTADPTCTLESDEVCVILENGQ NGKVLVYRNPGLHFGDIHVLTAKYVE+LVPVVGNA
Sbjct: 647  LMGTADPTCTLESDEVCVILENGQINGKVLVYRNPGLHFGDIHVLTAKYVEELVPVVGNA 706

Query: 663  KYAIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPWRPSPSMEGITHKKP 722
            KYAIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYF+PGEPWRPSPSMEGIT+KKP
Sbjct: 707  KYAIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFRPGEPWRPSPSMEGITNKKP 766

Query: 723  IEFSSEDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAKIL 782
             EFS+EDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGE+ KEERNRVRAKIL
Sbjct: 767  NEFSAEDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGEDRKEERNRVRAKIL 826

Query: 783  QLINIYYDALDAPKKGGKKIEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVNMYQE 842
            QLINIYYDALDAPKKGGKKIEVPKHLKA T PHF ERGKNSY STSILGQIFDTVNMYQE
Sbjct: 827  QLINIYYDALDAPKKGGKKIEVPKHLKAGTLPHFMERGKNSYTSTSILGQIFDTVNMYQE 886

Query: 843  EVPDVEVQKLPCFEDEVPDYTFMKWKFLYEMYRKDMCDALQLDPEAKNVAAEATIKKYKE 902
            EVP++EVQKLPCFED+VPD   MKWKF YEMYR +M DA+QLDP+AKNVAAEATIKKYKE
Sbjct: 887  EVPNIEVQKLPCFEDQVPDDILMKWKFHYEMYRAEMKDAMQLDPDAKNVAAEATIKKYKE 946

Query: 903  ILYGAEELEGSPRSIEEVYMEALAIYQVAYEYAMSHGTVSHCGFAWRVGGSALFKLCARK 957
            ILYGAEELEGSPR +EEV  EALAIY V Y++AMSHG+V +C FAWRV GSALFKLCARK
Sbjct: 947  ILYGAEELEGSPRWLEEVCKEALAIYHVTYDHAMSHGSVRYCSFAWRVAGSALFKLCARK 1006

BLAST of Sed0013258 vs. ExPASy TrEMBL
Match: A0A6J1L5Q2 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111499362 PE=3 SV=1)

HSP 1 Score: 1583.2 bits (4098), Expect = 0.0e+00
Identity = 797/979 (81.41%), Postives = 858/979 (87.64%), Query Frame = 0

Query: 1    MTLDALSKISSRPVRSLSGFIMHLVRNDPCSTPPNKMFAVSPQRSFSSSSSSSPSTGRVS 60
            M LD+L KISS  +R+LSGFIMH+VRNDPC +P NKM AV     FS  SS S STG VS
Sbjct: 45   MALDSLRKISSCSIRNLSGFIMHMVRNDPCISPQNKMVAV-----FSPHSSPS-STGCVS 104

Query: 61   QIQSPSTCSDASYQSPSSCRVYSPQGLGTAERTSFQPPTPEKPQRFSYSVSDRVRIPEYV 120
            Q+ SPSTCS + YQS SSCRVYSPQ  GTAE+T F+P TP+K   +S SVSDRV+IP++V
Sbjct: 105  QLHSPSTCSASLYQSLSSCRVYSPQ--GTAEKTKFEPSTPDKSAIYSSSVSDRVQIPQFV 164

Query: 121  ALGELEFRKAFLILSYIGGESLESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKEDR 180
            ALGELEFR+AFLILSYIGGESLE +TTADQI+SLSQ  M++FE EVW  FG+KY NKEDR
Sbjct: 165  ALGELEFRRAFLILSYIGGESLERVTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDR 224

Query: 181  RVYIDWDRRKTHIYHCYVSLDGRYKFKGPFLNNTKTHLQRVLGDDNVLLVKFAEDAPDRC 240
            RVY+DWDRRKTHIYHCYV+LDG Y FKGPFLN+TKTHLQRVLGDDNVL+VKFAED  DRC
Sbjct: 225  RVYVDWDRRKTHIYHCYVALDGSYSFKGPFLNHTKTHLQRVLGDDNVLMVKFAEDVSDRC 284

Query: 241  SPSHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESDA 300
            S SHPG  FYAY KIARDGILLGLRRY+FFVFKDGGKEEKKKNPTTSAVKCYFVRMES A
Sbjct: 285  SISHPGGSFYAYKKIARDGILLGLRRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAA 344

Query: 301  DSDKNEPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQRI 360
              DK +PYKLSNRTIFE RSLFMH H VSSVAS+MARFSLILSKT+ LKIDLS+VNVQRI
Sbjct: 345  YFDKLQPYKLSNRTIFEARSLFMHPHTVSSVASYMARFSLILSKTIKLKIDLSSVNVQRI 404

Query: 361  GDIPCRDAFGNVIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRTPIFEE 420
             DIPC+D  GNV+YRDGKPLIHTDGTGFISEDLALECP+NVFKGQA+H AD+KR P FE 
Sbjct: 405  TDIPCKDVNGNVMYRDGKPLIHTDGTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEV 464

Query: 421  FQNKTFQRTLPGLELREP------------------------LPSRTIQIRDSMIKVEID 480
            FQNKT Q  LPGLELREP                        LPSRTIQIR+SMIKVE D
Sbjct: 465  FQNKTSQVILPGLELREPPLLIQFRLFYNGLAVKGTFLVNKQLPSRTIQIRNSMIKVEKD 524

Query: 481  PKLENAEMVNSLEVVGTSNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFS 540
            P LENAE  NSLEVVGTSNPPKR ++SRNLIALLNYGGVPREYFMNIL+DAL DV+G+FS
Sbjct: 525  PNLENAETENSLEVVGTSNPPKRAYISRNLIALLNYGGVPREYFMNILVDALRDVQGLFS 584

Query: 541  SKRAALRVSINNGDMDDFLVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDC 600
            SKRAALRVSINNGDMDDFLVARMILSG+PLDESYLQYRLSMLL+EEKKSL+SGRLHVPDC
Sbjct: 585  SKRAALRVSINNGDMDDFLVARMILSGIPLDESYLQYRLSMLLREEKKSLRSGRLHVPDC 644

Query: 601  YYLMGTADPTCTLESDEVCVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVG 660
            YYLMGTADPTCTLES+EVCVILENGQ NGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVG
Sbjct: 645  YYLMGTADPTCTLESNEVCVILENGQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVG 704

Query: 661  NAKYAIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPWRPSPSMEGITHK 720
            NAKY IFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYF+PG+PWRPSPSME IT+ 
Sbjct: 705  NAKYGIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFRPGKPWRPSPSMEVITNI 764

Query: 721  KPIEFSSEDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAK 780
            K  EFS+EDLE+ELFKLFLT RFQPSYAKSVAADNW+ALMDQLLMLGE+ KEERNR+RAK
Sbjct: 765  KSNEFSAEDLEEELFKLFLTARFQPSYAKSVAADNWVALMDQLLMLGEDCKEERNRLRAK 824

Query: 781  ILQLINIYYDALDAPKKGGKKIEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVNMY 840
            ILQLINIYYDALDAPKKGGK+IEVPKHLKA T PHF ERGK SY STSILGQIFDTVNM+
Sbjct: 825  ILQLINIYYDALDAPKKGGKEIEVPKHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMF 884

Query: 841  QEEVPDVEVQKLPCFEDEVPDYTFMKWKFLYEMYRKDMCDALQLDPEAKNVAAEATIKKY 900
            QEE P++EVQKLPCFEDEV D T MKWKF YEMYRK+M DALQLDP+AKNVAAEATIKKY
Sbjct: 885  QEESPNIEVQKLPCFEDEVADDTLMKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKY 944

Query: 901  KEILYGAEELEGSPRSIEEVYMEALAIYQVAYEYAMSHGTVSHCGFAWRVGGSALFKLCA 956
            KEILYGAEELEGSPR +EEVY EALAIY V YEYAMSHG V +C FAWRV GSALF LCA
Sbjct: 945  KEILYGAEELEGSPRWMEEVYKEALAIYHVTYEYAMSHGCVRYCSFAWRVAGSALFNLCA 1004

BLAST of Sed0013258 vs. ExPASy TrEMBL
Match: A0A6J1L2X5 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111499362 PE=3 SV=1)

HSP 1 Score: 1578.1 bits (4085), Expect = 0.0e+00
Identity = 797/981 (81.24%), Postives = 858/981 (87.46%), Query Frame = 0

Query: 1    MTLDALSKISSRPVRSLSGFIMHLVRNDPCSTPPNKMFAVSPQRSFSSSSSSSPSTGRVS 60
            M LD+L KISS  +R+LSGFIMH+VRNDPC +P NKM AV     FS  SS S STG VS
Sbjct: 45   MALDSLRKISSCSIRNLSGFIMHMVRNDPCISPQNKMVAV-----FSPHSSPS-STGCVS 104

Query: 61   QIQSPSTCSDASYQSPSSCRVYSPQGLGTAERTSFQPPTPEKPQRFSYSVSDRVRIPEYV 120
            Q+ SPSTCS + YQS SSCRVYSPQ  GTAE+T F+P TP+K   +S SVSDRV+IP++V
Sbjct: 105  QLHSPSTCSASLYQSLSSCRVYSPQ--GTAEKTKFEPSTPDKSAIYSSSVSDRVQIPQFV 164

Query: 121  ALGELEFRKAFLILSYIGGESLESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKEDR 180
            ALGELEFR+AFLILSYIGGESLE +TTADQI+SLSQ  M++FE EVW  FG+KY NKEDR
Sbjct: 165  ALGELEFRRAFLILSYIGGESLERVTTADQIQSLSQLAMEKFESEVWKAFGEKYVNKEDR 224

Query: 181  RVYIDWDRRKTHIYHCYVSLDGRYKFKGPFLNNTKTHLQRVLGDDNVLLVKFAEDAPDRC 240
            RVY+DWDRRKTHIYHCYV+LDG Y FKGPFLN+TKTHLQRVLGDDNVL+VKFAED  DRC
Sbjct: 225  RVYVDWDRRKTHIYHCYVALDGSYSFKGPFLNHTKTHLQRVLGDDNVLMVKFAEDVSDRC 284

Query: 241  SPSHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESDA 300
            S SHPG  FYAY KIARDGILLGLRRY+FFVFKDGGKEEKKKNPTTSAVKCYFVRMES A
Sbjct: 285  SISHPGGSFYAYKKIARDGILLGLRRYQFFVFKDGGKEEKKKNPTTSAVKCYFVRMESAA 344

Query: 301  DSDKNEPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQRI 360
              DK +PYKLSNRTIFE RSLFMH H VSSVAS+MARFSLILSKT+ LKIDLS+VNVQRI
Sbjct: 345  YFDKLQPYKLSNRTIFEARSLFMHPHTVSSVASYMARFSLILSKTIKLKIDLSSVNVQRI 404

Query: 361  GDIPCRDAFGNVIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRTPIFEE 420
             DIPC+D  GNV+YRDGKPLIHTDGTGFISEDLALECP+NVFKGQA+H AD+KR P FE 
Sbjct: 405  TDIPCKDVNGNVMYRDGKPLIHTDGTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEV 464

Query: 421  FQNKTFQRTLPGLELREP------------------------LPSRTIQIRDSMIKVEID 480
            FQNKT Q  LPGLELREP                        LPSRTIQIR+SMIKVE D
Sbjct: 465  FQNKTSQVILPGLELREPPLLIQFRLFYNGLAVKGTFLVNKQLPSRTIQIRNSMIKVEKD 524

Query: 481  PKLENAEMVNSLEVVGTSNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFS 540
            P LENAE  NSLEVVGTSNPPKR ++SRNLIALLNYGGVPREYFMNIL+DAL DV+G+FS
Sbjct: 525  PNLENAETENSLEVVGTSNPPKRAYISRNLIALLNYGGVPREYFMNILVDALRDVQGLFS 584

Query: 541  SKRAALRVSINNGDMDDFLVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDC 600
            SKRAALRVSINNGDMDDFLVARMILSG+PLDESYLQYRLSMLL+EEKKSL+SGRLHVPDC
Sbjct: 585  SKRAALRVSINNGDMDDFLVARMILSGIPLDESYLQYRLSMLLREEKKSLRSGRLHVPDC 644

Query: 601  YYLMGTADPTCTLESDEVCVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVG 660
            YYLMGTADPTCTLES+EVCVILENGQ NGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVG
Sbjct: 645  YYLMGTADPTCTLESNEVCVILENGQINGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVG 704

Query: 661  NAKYAIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPWRPSPSMEGITHK 720
            NAKY IFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYF+PG+PWRPSPSME IT+ 
Sbjct: 705  NAKYGIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFRPGKPWRPSPSMEVITNI 764

Query: 721  KPIEFSSEDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAK 780
            K  EFS+EDLE+ELFKLFLT RFQPSYAKSVAADNW+ALMDQLLMLGE+ KEERNR+RAK
Sbjct: 765  KSNEFSAEDLEEELFKLFLTARFQPSYAKSVAADNWVALMDQLLMLGEDCKEERNRLRAK 824

Query: 781  ILQLINIYYDALDAPKKGGKKIEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVNMY 840
            ILQLINIYYDALDAPKKGGK+IEVPKHLKA T PHF ERGK SY STSILGQIFDTVNM+
Sbjct: 825  ILQLINIYYDALDAPKKGGKEIEVPKHLKAGTFPHFMERGKKSYTSTSILGQIFDTVNMF 884

Query: 841  QEEVPDVEVQKLPCFEDEVPDYTFMKWKFLYEMYRKDMCDALQLDPEAKNVAAEATIKKY 900
            QEE P++EVQKLPCFEDEV D T MKWKF YEMYRK+M DALQLDP+AKNVAAEATIKKY
Sbjct: 885  QEESPNIEVQKLPCFEDEVADDTLMKWKFQYEMYRKEMRDALQLDPDAKNVAAEATIKKY 944

Query: 901  KE--ILYGAEELEGSPRSIEEVYMEALAIYQVAYEYAMSHGTVSHCGFAWRVGGSALFKL 956
            KE  ILYGAEELEGSPR +EEVY EALAIY V YEYAMSHG V +C FAWRV GSALF L
Sbjct: 945  KELQILYGAEELEGSPRWMEEVYKEALAIYHVTYEYAMSHGCVRYCSFAWRVAGSALFNL 1004

BLAST of Sed0013258 vs. ExPASy TrEMBL
Match: A0A5D3CF42 (RNA-dependent RNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003000 PE=3 SV=1)

HSP 1 Score: 1565.1 bits (4051), Expect = 0.0e+00
Identity = 784/978 (80.16%), Postives = 855/978 (87.42%), Query Frame = 0

Query: 3    LDALSKIS-SRPVRSLSGFIMHLVRNDPCSTPPNKMFAVSPQRSFSSSSSSSPSTGRVSQ 62
            LDAL KIS S PVR+LSGFI+H+VR D C++P NKM  VSP +S SS         RVSQ
Sbjct: 47   LDALHKISCSSPVRNLSGFILHMVRKDSCASPQNKMVRVSPHQSPSSCC-------RVSQ 106

Query: 63   IQSPSTCSDASYQSPSSCRVYSPQGLGTAERTSFQPPTPEKPQRFSYSVSDRVRIPEYVA 122
            +QSPSTCS + +QS S+  + S QGLGTAE  SFQPPTPEK   FS SVSDR RI ++VA
Sbjct: 107  LQSPSTCSVSLHQSLSTFSLSSGQGLGTAENASFQPPTPEKSGSFSSSVSDRARISQFVA 166

Query: 123  LGELEFRKAFLILSYIGGESLESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKEDRR 182
            LGELEFRKAFL+LSYIGGESLE +TTA+QI+SLSQ  M++FE EVW +FGKKY   E+RR
Sbjct: 167  LGELEFRKAFLLLSYIGGESLERVTTAEQIQSLSQLAMEKFEDEVWKIFGKKYITNEERR 226

Query: 183  VYIDWDRRKTHIYHCYVSLDGRYKFKGPFLNNTKTHLQRVLGDDNVLLVKFAEDAPDRCS 242
            +Y+DWDRRKTHIYHCYV+LDG Y+FKGPFLNNTKTHLQRVLGDDNVL+VKFAED  D   
Sbjct: 227  MYVDWDRRKTHIYHCYVALDGSYRFKGPFLNNTKTHLQRVLGDDNVLMVKFAEDKSDTHL 286

Query: 243  PSHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESDAD 302
            P+H G  FYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESDA 
Sbjct: 287  PNHTGGSFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESDAY 346

Query: 303  SDKNEPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQRIG 362
             DK EPYKLSNRT+FE RSLFMHAHMVSS+AS+MARFSLILSKT+ LKIDLSTV VQRIG
Sbjct: 347  VDKIEPYKLSNRTVFEARSLFMHAHMVSSIASYMARFSLILSKTIKLKIDLSTVKVQRIG 406

Query: 363  DIPCRDAFGNVIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRTPIFEEF 422
            DIPC+D +GNVIYRDGKPLIHTDGTGFISEDLALECP+NVFKGQA+H AD+KR P FE F
Sbjct: 407  DIPCKDIYGNVIYRDGKPLIHTDGTGFISEDLALECPMNVFKGQAKHDADLKRIPAFEGF 466

Query: 423  QNKTFQRTLPGLELREP------------------------LPSRTIQIRDSMIKVEIDP 482
            QNKT   TLPGLELREP                        LP RTIQIRDSMIKVEIDP
Sbjct: 467  QNKTLPLTLPGLELREPPLLIQFRLFYNGLAVKGTFLVNKQLPPRTIQIRDSMIKVEIDP 526

Query: 483  KLENAEMVNSLEVVGTSNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFSS 542
             L N E  NSLE+VGTSNPPKRTFLSRNLIALLNYGGVPREYFM IL+DAL DV+GVFSS
Sbjct: 527  DLVNFETENSLELVGTSNPPKRTFLSRNLIALLNYGGVPREYFMTILVDALKDVQGVFSS 586

Query: 543  KRAALRVSINNGDMDDFLVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDCY 602
            KRAALRVSINNG+MDDFLVARMIL+G+PLDESYLQYRLS+LLKEEKKSLKSGRLH+P+CY
Sbjct: 587  KRAALRVSINNGEMDDFLVARMILAGIPLDESYLQYRLSVLLKEEKKSLKSGRLHMPECY 646

Query: 603  YLMGTADPTCTLESDEVCVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGN 662
            YLMGT DPTCTLES EVCVILENGQ NGKVLVYRNPGLHFGDIHVLTAKYVE+LVPVVGN
Sbjct: 647  YLMGTVDPTCTLESGEVCVILENGQINGKVLVYRNPGLHFGDIHVLTAKYVEELVPVVGN 706

Query: 663  AKYAIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPWRPSPSMEGITHKK 722
            AKYAIFFS KGPRSVADEIAGGDFDGDMYWVSRNSQLLEYF+P EPWRPSPS E +T++K
Sbjct: 707  AKYAIFFSSKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFRPCEPWRPSPSTEVVTNRK 766

Query: 723  PIEFSSEDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAKI 782
            P EFS E+LE+ELFKLFL+TRFQPSYAKSVAADNWLALMDQ LMLGE  KEERNR+RAKI
Sbjct: 767  PKEFSVEELENELFKLFLSTRFQPSYAKSVAADNWLALMDQFLMLGEERKEERNRIRAKI 826

Query: 783  LQLINIYYDALDAPKKGGKKIEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVNMYQ 842
            LQLINIYYDALDAPKKGGKKIEVPKHLKA T PHF ERGKNSY+STSILGQIFDTVNMYQ
Sbjct: 827  LQLINIYYDALDAPKKGGKKIEVPKHLKAGTLPHFMERGKNSYVSTSILGQIFDTVNMYQ 886

Query: 843  EEVPDVEVQKLPCFEDEVPDYTFMKWKFLYEMYRKDMCDALQLDPEAKNVAAEATIKKYK 902
            EEVP++EVQKLPCFE+EVP+Y FMKWKFLYE+YRKDM DA+QLDP+AKN+AAEATIKKYK
Sbjct: 887  EEVPNIEVQKLPCFEEEVPEYIFMKWKFLYELYRKDMVDAMQLDPDAKNIAAEATIKKYK 946

Query: 903  EILYGAEELEGSPRSIEEVYMEALAIYQVAYEYAMSHGTVSHCGFAWRVGGSALFKLCAR 956
            EILYGAEELEGSPRS ++VY EALAIYQV Y++AMSH +V +C FAW+V GSALFKL A 
Sbjct: 947  EILYGAEELEGSPRSNDDVYQEALAIYQVTYDHAMSH-SVRNCVFAWKVAGSALFKLYAI 1006

BLAST of Sed0013258 vs. TAIR 10
Match: AT2G19930.1 (RNA-dependent RNA polymerase family protein )

HSP 1 Score: 831.2 bits (2146), Expect = 8.2e-241
Identity = 437/903 (48.39%), Postives = 598/903 (66.22%), Query Frame = 0

Query: 76  PSSCRVYSPQGLGTA----ERTSFQPPTPEKPQRFSYSVSDRVRIPEYVALGELEFRKAF 135
           P      SP G   +    E  S     P      S      + IP+ VALGELEF+K F
Sbjct: 84  PQPANSLSPSGRHVSRVLQEEMSVDSDAPSPKSLKSEDKGGSLHIPQLVALGELEFKKVF 143

Query: 136 LILSYIGGESLESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKEDRRVYIDWDRRKT 195
           L+LSYI G+ +  + TAD+IR     PM  +E  VW+  G+ Y  ++DRR+ + WD  KT
Sbjct: 144 LLLSYIPGQHVGQVITADEIRLWKDLPMVEYEAAVWDRLGRHYCPQKDRRM-LQWDSGKT 203

Query: 196 HIYHCYVSLDGRYKFK------GPFLNNTKTHLQRVLGDDNVLLVKFAEDAPDRCSPSHP 255
           H Y C V+ +G Y FK      GP L +T THL +VLGDDNVL VKFA+    + S ++ 
Sbjct: 204 HYYQCNVAPNGSYTFKVLSALQGPLLEHTGTHLHKVLGDDNVLTVKFAD--VQKSSSTYS 263

Query: 256 GDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESDADSDKN 315
            D ++ Y  IA++GI++GLRRY+FFVFKDGGKEEKKK+ +T  VKCYF+R +S A  D  
Sbjct: 264 IDHYFTYKGIAKNGIMIGLRRYQFFVFKDGGKEEKKKDLSTKKVKCYFIRTDSTAFYDMQ 323

Query: 316 EPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQRIGDIPC 375
            PY L+ ++I+E R  FMH H   ++A++MARFSLILSKT  L++D++ +   +I DI C
Sbjct: 324 NPYILTGKSIYEARMHFMHVHRAPTLANYMARFSLILSKTKTLEVDMTGITFDQIDDIHC 383

Query: 376 RDAFG-NVIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRT-----PIFE 435
            D  G +V+ ++ KP IH+DGTG+ISEDLA  CP+N+FKG+      I+       P+  
Sbjct: 384 HDQDGKDVLDKNKKPCIHSDGTGYISEDLARMCPLNIFKGKCLRSESIQEACYQDPPLLI 443

Query: 436 EFQNKTFQRTLPG-LELREPLPSRTIQIRDSMIKVEIDPKLENAEMVNSLEVVGTSNPPK 495
           +F+       + G   L + L  RT+Q+R SMIKV  DP L N    N+LEVV TSNPPK
Sbjct: 444 QFRMFYDGYAVKGTFLLNKKLCPRTVQVRPSMIKVSKDPSLSNFSTFNALEVVTTSNPPK 503

Query: 496 RTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFSSKRAALRVSINNGDMDDFLVAR 555
           RT LS+NL+ALL+YGG+P E+F++IL++ L + + +F +KRAAL  ++N G+MDD   A+
Sbjct: 504 RTKLSKNLVALLSYGGIPNEFFLDILLNTLEESKSIFYNKRAALNAALNYGEMDDQNAAQ 563

Query: 556 MILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDCYYLMGTADPTCTLESDEVCVIL 615
           MIL G+PLDE +L+  LS+LLK EK  LK+G+L V + YYLMGT DPT  L+ DEVCVIL
Sbjct: 564 MILVGIPLDEPHLKNYLSILLKTEKNDLKAGKLPVTESYYLMGTVDPTGALKEDEVCVIL 623

Query: 616 ENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYAIFFSCKGPRSVADEIAG 675
           E+GQ +G+VLVYRNPGLHFGDIH+L A YV+ L   VGN+K+A+FF  KGPRS+ DEIAG
Sbjct: 624 ESGQISGEVLVYRNPGLHFGDIHILKATYVKALEEYVGNSKFAVFFPQKGPRSLGDEIAG 683

Query: 676 GDFDGDMYWVSRNSQLLEYFKPGEPW-RPSPSMEGITHKKPIEFSSEDLEDELFKLFLTT 735
           GDFDGDMY++SRN +LLE FKP EPW   +P  +  + + P + S E+LE+ELF++FLT 
Sbjct: 684 GDFDGDMYFISRNPELLENFKPSEPWVSLTPPSKSNSGRAPSQLSPEELEEELFEMFLTA 743

Query: 736 RFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAKILQLINIYYDALDAPKKGGKK 795
            F  S    +AAD+WL +MD+ L+LG++  EE+  ++ K+L+LI+IYYDALDAPKK G K
Sbjct: 744 GFHASNVIGIAADSWLTIMDRFLILGDDRAEEKAEMKKKMLELIDIYYDALDAPKK-GDK 803

Query: 796 IEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVNMY--QEEVPDVEVQKLPCFEDE- 855
           + +P  LK    PH+ ER K  + STSILG IFD V     +E  P  E+ KLPCFEDE 
Sbjct: 804 VYLPNKLKPDIFPHYMERDK-KFQSTSILGLIFDFVKSQTTEEPSPSSEISKLPCFEDEP 863

Query: 856 VPDYTFMKWKFLYEMYRKDMCDALQLDPEAKNVAAEATIKKYKEILYGAEELEGSPRSIE 915
           V ++   K +  Y+ YR +M  A++ D   K+ +A   I++YK+  YGA   E S +S+E
Sbjct: 864 VSEFHMQKCRLWYDNYRTEMTQAMKTD---KDESANEVIQRYKQEFYGAAGFEDSKKSLE 923

Query: 916 EVYMEALAIYQVAYEYAMSHGTVSHCGFAWRVGGSALFKL-CARKHSEKSFLCLPSVMRE 957
           E+Y +ALA+Y++ Y+YA+ H  VS C F W+V G  L +    +K  EK  +C PSV++E
Sbjct: 924 ELYPQALALYKIVYDYAI-HAGVSKCRFVWKVAGPVLCRFYLNKKMQEKCLVCAPSVLKE 977

BLAST of Sed0013258 vs. TAIR 10
Match: AT2G19910.1 (RNA-dependent RNA polymerase family protein )

HSP 1 Score: 801.6 bits (2069), Expect = 6.9e-232
Identity = 453/979 (46.27%), Postives = 612/979 (62.51%), Query Frame = 0

Query: 1   MTLDALSKISSRP-VRSLSGFIMHLVRNDPCSTPPNKMFAVSPQRSFSSSSSSSPSTGRV 60
           + L+ L K+ ++P +++L G IM+ V+             V+   S   S   SP     
Sbjct: 53  LALETLRKVFNKPYLKTLDGLIMYFVKG-----------TVTVDGSPRLSPGESP----- 112

Query: 61  SQIQSPSTCSDASYQSPSSCRVYSPQGLGTAERTSFQPPTPEKPQRFSYSVSDRVRIPEY 120
             +QSP T       +  SCR      L        + P+P+  +R     S    IP  
Sbjct: 113 --VQSPRT------PAKKSCRASQDVSL------DLETPSPKFMKREENGGSK--YIPPL 172

Query: 121 VALGELEFRKAFLILSYIGGESL-ESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKE 180
           +ALGELEF+KAFL+LSYIGGESL E + + DQIR     PM  +E  VWN  G++Y + +
Sbjct: 173 LALGELEFKKAFLLLSYIGGESLVEEVISGDQIRKWKDLPMVSYEAAVWNRLGQRYCSPK 232

Query: 181 DRRVYIDWDRRKTHIYHCYVSLDGRYKFKGPFLNNTKTHLQRVLGDDNVLLVKFAEDAPD 240
           +RR  ++ D   TH Y C+V+ DG YKFKG  L NT THL +VLGDDNVL VKF +    
Sbjct: 233 ERRRPLEGDSGMTHYYQCHVATDGSYKFKGHLLENTGTHLHKVLGDDNVLTVKFDKVLG- 292

Query: 241 RCSPSHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMES 300
               ++  D +  Y  IA++GI++GLRRYRFFVFKDGGKEEKKK+ +T  VKCYF+R +S
Sbjct: 293 --VETYCNDLYSTYKGIAKNGIMVGLRRYRFFVFKDGGKEEKKKDVSTKGVKCYFIRTDS 352

Query: 301 DADSDKNEPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQ 360
            A  D   PY  + +++ E R  FMH + +SS+ ++M+RFSLILSKT  L++D++ +  +
Sbjct: 353 TASIDMQNPYIFAGKSMHEARMHFMHVNTLSSLPNYMSRFSLILSKTKTLEVDMTGITFE 412

Query: 361 RIGDIPCRDAFG-NVIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRTPI 420
           +I DI C D    +V+ ++GKP IH+DGTG+ISEDLA  CP+N+FKG++    +I+   +
Sbjct: 413 QIDDIHCHDQDDKDVLDKNGKPCIHSDGTGYISEDLARMCPVNIFKGKSMRSNNIQSKNL 472

Query: 421 FEEFQNKTFQRTLPGLELR---------------EPLPSRTIQIRDSMIKVEIDPKLENA 480
             E Q    Q     ++ R               + LP RT+Q+R SMIKV  D  L N 
Sbjct: 473 NFEGQGPCGQEPPLLIQFRIFYNGYAVKGTFLTNKKLPPRTVQVRPSMIKVYEDRTLSNL 532

Query: 481 EMVNSLEVVGTSNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFSSKRAAL 540
              NSLEVV TSNPP++  LSRNL+ALL+YGGVP ++F+NIL + L + + +F S+RAA 
Sbjct: 533 STFNSLEVVTTSNPPRKARLSRNLVALLSYGGVPNDFFLNILRNTLEESKTIFYSERAAF 592

Query: 541 RVSINNGDMDDFLVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDCYYLMGT 600
           + +IN G  DD   A MIL G+PLDE YL+ RLS LLK E+ +LK+GR  + + YY+MGT
Sbjct: 593 KAAINYG--DDQYTADMILVGIPLDEPYLKDRLSYLLKTERNALKAGRFPIDESYYIMGT 652

Query: 601 ADPTCTLESDEVCVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYAI 660
            DPT  L+ +E+CVIL +GQ +G VLVYRNPGLHFGDIHVL A YV+ L   VGNAK+A+
Sbjct: 653 VDPTGELKENEICVILHSGQISGDVLVYRNPGLHFGDIHVLKATYVKALEDYVGNAKFAV 712

Query: 661 FFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPWRPSPSMEGI-THKKPIEF 720
           FF  KGPRS+ DEIAGGDFDGDMY++SRN +LLE+FKP EPW  S     I   +KP E 
Sbjct: 713 FFPQKGPRSLGDEIAGGDFDGDMYFISRNPKLLEHFKPSEPWVSSSKPSKIYCGRKPSEL 772

Query: 721 SSEDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAKILQLI 780
           S E+LE+ELFK+FL  RF       +AAD WL +MD  L LG+   +E+   +  IL+LI
Sbjct: 773 SEEELEEELFKMFLKARFCKRDVIGMAADCWLGIMDPFLTLGDESAKEKYERKKNILKLI 832

Query: 781 NIYYDALDAPKKGGKKIEVPKHLKAVTSPHFFERG-KNSYISTSILGQIFDTVNMYQ-EE 840
           +IYYDALDAPKKG  K+++P  L+    PH+ ER  K  + STSILG IFDTV+ +  EE
Sbjct: 833 DIYYDALDAPKKGA-KVDLPPDLEIKNFPHYMERDPKRDFRSTSILGLIFDTVDSHNAEE 892

Query: 841 VPDVEVQKLPCFEDE-VPDYTFMKWKFLYEMYRKDMCDA-LQLDPEAKNVAAEATIKKYK 900
            P  E+ KL  FEDE VP     K+   YE YR +M  A ++ D   +N      I++YK
Sbjct: 893 PPPSEISKLWYFEDEPVPKSHMDKFTSWYENYRSEMSQAMMETDKVKRNQLTNEVIQRYK 952

Query: 901 EILYGAEELEGSPRSIEEVYMEALAIYQVAYEYAMSHGTVSHCGFAWRVGGSALFKLCAR 957
           +  YGA   E S +S+EE+Y +ALA+Y V Y+YA+  G V+ C FAW V G  L K   +
Sbjct: 953 QDFYGAAGFEDSNKSLEELYPQALALYNVVYDYAIQEG-VAKCTFAWNVAGPVLCKFYLK 992

BLAST of Sed0013258 vs. TAIR 10
Match: AT2G19920.1 (RNA-dependent RNA polymerase family protein )

HSP 1 Score: 729.9 bits (1883), Expect = 2.6e-210
Identity = 385/797 (48.31%), Postives = 532/797 (66.75%), Query Frame = 0

Query: 120 VALGELEFRKAFLILSYIGGESLESITTADQIRSLSQFPMDRFEVEVWNLFGKKYTNKED 179
           +ALGELEF+KAFL+L+Y+GG+SL  + + D+IR     PM  +E  VW   G     + +
Sbjct: 147 LALGELEFKKAFLLLTYLGGKSLGEVISGDEIRQWKDLPMVAYERAVWFKLG-----QNE 206

Query: 180 RRVYIDWDRRKTHIYHCYVSLDGRYKFKGPFLNNTKTHLQRVLGDDNVLLVKFAEDAPDR 239
            R+ ++ D  KTH Y C+V+ DG Y+ KG FL NT THL +VLGDDNVL V+F +   + 
Sbjct: 207 ERMQLESDSGKTHYYQCHVAPDGSYRLKGYFLENTGTHLHKVLGDDNVLTVRFDQLPKES 266

Query: 240 CSPSHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKKNPTTSAVKCYFVRMESD 299
               +P   +  Y +IA++GI++GLRRY+FFVFKDGGK EKKK  +T  VKCYF+R  S 
Sbjct: 267 TYCDNP---YSKYKEIAKNGIMVGLRRYQFFVFKDGGKAEKKKRNSTKQVKCYFIRTGST 326

Query: 300 ADSDKNEPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILSKTVNLKIDLSTVNVQR 359
           A SD   PY LS  +I E R  FMH H + S A++MARFSLILSKT  L++D++ + V +
Sbjct: 327 ASSDMENPYILSGMSIHEARMHFMHVHTLPSPANYMARFSLILSKTKKLEVDMTEITVMQ 386

Query: 360 IGDIPCRDAFGN-VIYRDGKPLIHTDGTGFISEDLALECPINVFKGQARHGADIKRT--- 419
           I DI C D   N V+ ++GKP IH+DGTG+ISEDLA  CP+N+FKG++    +I+ T   
Sbjct: 387 IDDIHCHDQSNNDVLDKNGKPRIHSDGTGYISEDLARMCPLNIFKGKSMRSNNIQGTCVQ 446

Query: 420 --PIFEEFQNKTFQRTLPGL-ELREPLPSRTIQIRDSMIKVEIDPKLENAEMVNSLEVVG 479
             P+  + +       + G+  L + LP +T+Q+R SMIKV  D  L N    NSLEVV 
Sbjct: 447 EPPLLIQIRMFNDGSAVKGIFLLNKNLPPQTVQVRPSMIKVYKDKNLSNFSTFNSLEVVT 506

Query: 480 TSNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFSSKRAALRVSINNGDMD 539
           TSNPPKR  LS+NL+ALL+YGGVP ++F++IL++ L   + +F   RAA + +++ G+MD
Sbjct: 507 TSNPPKRAKLSKNLVALLSYGGVPNDFFLDILLNTLEKKKTIFFKVRAAGKAALHYGNMD 566

Query: 540 DFLVARMILSGVPLDESYLQYRLSMLLKEEKKSLKSGRLHVPDCYYLMGTADPTCTLESD 599
           D    +MI++G+PLDE YL++ LS LLK EK  LK+G+L + + YYLMGT DPT  L+ D
Sbjct: 567 DKNALQMIMAGIPLDEPYLKHYLSKLLKLEKDDLKAGKLPIDESYYLMGTVDPTGELKED 626

Query: 600 EVCVILENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYAIFFSCKGPRSV 659
           EV  + ++      VLVYRNPGLHFGDIH+L A YV+ L   VGN+KY +FF  KGPRS+
Sbjct: 627 EVSGLAKSQD----VLVYRNPGLHFGDIHILKATYVKSLEQYVGNSKYGVFFPQKGPRSL 686

Query: 660 ADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPW-RPSPSMEGITHKKPIEFSSEDLEDELF 719
            DEIAGGDFDGDMY++SRN +LLE++KP EPW   SP  +  T ++P E S E LE+ELF
Sbjct: 687 GDEIAGGDFDGDMYFISRNPKLLEHYKPSEPWVSSSPRSKIYTGRQPSELSPEQLEEELF 746

Query: 720 KLFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAKILQLINIYYDALDAP 779
           K+FL T F PS     AAD+WLA+MD+ L LG+ + +E+  ++ K+L+L +IYYDA+DAP
Sbjct: 747 KIFLKTGFSPSSVIGQAADSWLAIMDRFLTLGDENVKEKAEMKKKMLKLTDIYYDAIDAP 806

Query: 780 KKGGKKIEVPKHLKAVTSPHFFERGKNSYISTSILGQIFDTVNMYQ-EEVPDVEVQKLPC 839
           K  G ++ +P  +K    PH+ ER K ++ STSILG IFDTV+ +  E+     + KL C
Sbjct: 807 KT-GTEVNLPLDVKVDLFPHYMERNK-TFKSTSILGLIFDTVDFHNAEDTTPSGISKLQC 866

Query: 840 FEDE-VPDYTFMKWKFLYEMYRKDMCDALQLDPEAKNVAAEATIKKYKEILYGAEELEGS 899
           FEDE V ++   K K  ++ YRK+MC A+  D +    +    I+KYK+  Y A   + S
Sbjct: 867 FEDEPVSEFDMEKCKLWHKDYRKEMCQAMNSDDDD---SCNEVIQKYKQEFYSAAGFKES 926

Query: 900 PRSIEEVYMEALAIYQV 907
            + +EE+Y +ALA+Y V
Sbjct: 927 KKILEELYPKALALYNV 926

BLAST of Sed0013258 vs. TAIR 10
Match: AT4G11130.1 (RNA-dependent RNA polymerase 2 )

HSP 1 Score: 100.1 bits (248), Expect = 9.9e-21
Identity = 114/416 (27.40%), Postives = 180/416 (43.27%), Query Frame = 0

Query: 442 RTIQIRDSMIKVEIDPKLENAEMVNSLEVVGTSNPPKRTFLSRNLIALLNYGGVPREYFM 501
           R + +RDSM+K +      N  M+N      T + P   FL+R +I LL+  G+    F 
Sbjct: 600 RKLSLRDSMLKFD-----SNNRMLNVTR--WTESMP--CFLNREIICLLSTLGIEDAMFE 659

Query: 502 NILMDALGDVRGVFSSKRAALRVSIN-NGDMDDFLVARMILSG-VPLDESYLQYRLSMLL 561
            +    L  +  +   + AAL V    +G+    L+ +M+L G  P  E YL   L +  
Sbjct: 660 AMQAVHLSMLGNMLEDRDAALNVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHH 719

Query: 562 KEEKKSLKSG-RLHVPDCYYLMGTADPTCTLESDEVCVIL-------------------- 621
           + +   LKS  R+ VP    L+G  D    LE  +V V +                    
Sbjct: 720 ESQLSELKSRCRILVPKGRILIGCMDEMGILEYGQVYVRVTLTKAELKSRDQSYFRKIDE 779

Query: 622 ENGQFNGKVLVYRNPGLHFGDIHVLTAKYVEDLVPVVGNAKYAIFFSCKGPRSVADEIAG 681
           E     GKV+V +NP LH GDI VL A Y E      G     I F  KG R   +E +G
Sbjct: 780 ETSVVIGKVVVTKNPCLHPGDIRVLDAIY-EVHFEEKGYLD-CIIFPQKGERPHPNECSG 839

Query: 682 GDFDGDMYWVSRNSQLLEYFKPGEPWRPSPSMEGITHKKPIEFSSEDLEDELFKLFLTTR 741
           GD DGD ++VS + +++       P    P M+     +P     +   +E+ K F+   
Sbjct: 840 GDLDGDQFFVSWDEKII-------PSEMDPPMD-YAGSRPRLMDHDVTLEEIHKFFV--- 899

Query: 742 FQPSYAKSVAADNWLALMDQLLMLGENHKEERNRVRAKILQLINIYYDALDAPKKGGKKI 801
                       + L ++    ++  +   E+ R + K L+L N++  A+D  K G    
Sbjct: 900 -------DYMISDTLGVISTAHLVHADRDPEKARSQ-KCLELANLHSRAVDFAKTGA-PA 959

Query: 802 EVPKHLKAVTSPHFFER-GKNSYISTSILGQIFDTV-NMYQEEVPDVEVQKLPCFE 833
           E+P  LK    P F ER  K +YIS S+ G+++  V +   +  P+ E +    ++
Sbjct: 960 EMPYALKPREFPDFLERFEKPTYISESVFGKLYRAVKSSLAQRKPEAESEDTVAYD 984

BLAST of Sed0013258 vs. TAIR 10
Match: AT1G14790.1 (RNA-dependent RNA polymerase 1 )

HSP 1 Score: 95.5 bits (236), Expect = 2.4e-19
Identity = 152/666 (22.82%), Postives = 252/666 (37.84%), Query Frame = 0

Query: 184 IDWDRRKTHIYHCYVSLDGRYKFKGPFL---NNTKTHLQRVLGDDNVLLVKFAE------ 243
           I  D    ++Y   V+   R  F GP +   N    H  + +  +N L V F +      
Sbjct: 358 ISLDDGLVYMYRVQVT-PARVYFSGPEVNVSNRVLRHYSKYI--NNFLRVSFVDEDLEKV 417

Query: 244 ---DAPDRCSPSHPGDFFYAYNKIARDGILLGLRRYRFFVFKDGGKEEKKK---NPTTSA 303
              D   R S       +     + RDGI++G +++ F  F      E       P    
Sbjct: 418 RSMDLSPRSSTQRRTKLYDRIYSVLRDGIVIGDKKFEFLAFSSSQLRENSAWMFAPIDRI 477

Query: 304 VKCYFVRMESDADSDKNEPYKLSNRTIFEGRSLFMHAHMVSSVASFMARFSLILS---KT 363
              +      D D  +N                         VA + AR     S   +T
Sbjct: 478 TAAHIRAWMGDFDHIRN-------------------------VAKYAARLGQSFSSSRET 537

Query: 364 VNLKIDLSTVNVQRIGDIPCRDAFGNVIYRDGKPLIHTDGTGFISEDLALECPINVFKGQ 423
           +N++ D     ++ I D+         I   G   + +DG G IS + A +         
Sbjct: 538 LNVRSD----EIEVIPDVE--------IISLGTRYVFSDGIGKISAEFARK--------- 597

Query: 424 ARHGADIKRTPIFEEFQNKTFQ---RTLPGLELREPLPSRTIQIRDSMIKVEIDPKLENA 483
                 + R     EF    FQ       G+   +P  S+ + +R SM K E     EN 
Sbjct: 598 ------VARKCGLTEFSPSAFQIRYGGYKGVVAVDPNSSKKLSLRKSMSKFE----SENT 657

Query: 484 EMVNSLEVVGTSNPPKRTFLSRNLIALLNYGGVPREYFMNILMDALGDVRGVFSSKRAAL 543
           +    L+V+  S   +  +++R LI LL+  GV    F     + +  +  + +    A 
Sbjct: 658 K----LDVLAWSK-YQPCYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPLEAH 717

Query: 544 RV--SINNGDMDDFLVARMILSGVPLDESYL-----QYRLSMLLKEEKKSLKSGRLHVPD 603
                +  G+  + L A ++    P  E +L      +R S LL+   K+    R+ +  
Sbjct: 718 EALGLMAPGENTNILKALILCGYKPDAEPFLSMMLQNFRASKLLELRTKT----RIFISG 777

Query: 604 CYYLMGTADPTCTLESDEVCVILENGQ-------FNGKVLVYRNPGLHFGDIHVLTAKYV 663
              +MG  D T TLE  +V V   +           G V+V +NP LH GD+ VL A   
Sbjct: 778 GRSMMGCLDETRTLEYGQVVVQYSDPMRPGRRFIITGPVVVAKNPCLHPGDVRVLQAVN- 837

Query: 664 EDLVPVVGNAKYAIFFSCKGPRSVADEIAGGDFDGDMYWVSRNSQLLEYFKPGEPWRPSP 723
              VP + +    + F  KG R   +E +G D DGD+Y+V  + +L+       P R S 
Sbjct: 838 ---VPALNHMVDCVVFPQKGLRPHPNECSGSDLDGDIYFVCWDQELV-------PPRTSE 897

Query: 724 SMEGITHKKPIEFSSEDLEDELFKLFLTTRFQPSYAKSVAADNWLALMDQLLMLGENHKE 783
            M+     +P +    D+  E  + +    +  + +  + A+   A  D         KE
Sbjct: 898 PMD--YTPEPTQILDHDVTIEEVEEYF-ANYIVNDSLGIIANAHTAFAD---------KE 931

Query: 784 ERNRVRAKILQLINIYYDALDAPKKGGKKIEVPKHLKAVTSPHFFER-GKNSYISTSILG 814
                    ++L   +  A+D PK G   + +P+HL     P F E+  K +Y S +++G
Sbjct: 958 PLKAFSDPCIELAKKFSTAVDFPKTGVAAV-IPQHLYVKEYPDFMEKPDKPTYESKNVIG 931