Homology
BLAST of Sed0013143 vs. NCBI nr
Match:
XP_008447073.1 (PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X1 [Cucumis melo])
HSP 1 Score: 1363.2 bits (3527), Expect = 0.0e+00
Identity = 674/777 (86.74%), Postives = 724/777 (93.18%), Query Frame = 0
Query: 21 TLSISSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAWVAMVPSPN 80
T S S+SLFPGL+V+ KTK GALR FEDTKERI+KLF VELSVSAYDTAWVAMVPSPN
Sbjct: 13 TPSSSASLFPGLDVDTKTKTGALR--FEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPN 72
Query: 81 SLNKPLFPECIDWVLDNQHSDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGHNHITK 140
SLNKPLFPECI+WVLD+Q+ DGSWGILHDHQL+MKATLLSTLACVLTLKRWNIGH+H++K
Sbjct: 73 SLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSK 132
Query: 141 ALDFIKSNIASATDDKQQSPVGFNIIFPGMIEYAKDLNLNLPLAPMNLDALVQKKQLELR 200
AL FIKSNIASATD+ Q+SPVGF+IIFPGMIEYAKDLNLNLPLA MN+DALVQKK+LELR
Sbjct: 133 ALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALVQKKELELR 192
Query: 201 SCRSNFEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVTFMHGNDDACFN 260
SC SN EGGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SPSTTAV FMH NDD CFN
Sbjct: 193 SCCSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFN 252
Query: 261 YLSSLLQKFDGSVPSIYPLDIYARLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWMQGEE 320
YL S+LQKF SVP+IYPLDIYARLHMVD+LQKLGIDRHFK+EIRSVLDETYRCWMQGEE
Sbjct: 253 YLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEE 312
Query: 321 DIFLDVSTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGYLKDFSASLELFKASQII 380
+IFLD STCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGG+LKDF ASLELFKASQII
Sbjct: 313 NIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLELFKASQII 372
Query: 381 LHPDETVLENINSWTSRFLNHGLSSDSVCSDRTDRLVKQEAVNAFEFTYSASLERLLNKK 440
++PDE +LENINSWTSRFLNHGLS SV SDRT+RLVKQEAVNAFEF Y+++LERL NK+
Sbjct: 373 IYPDEFILENINSWTSRFLNHGLSGGSVHSDRTERLVKQEAVNAFEFPYNSTLERLSNKR 432
Query: 441 AIENYSGDTVRISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIENRLDK 500
A+E+YSGD VRISK+TYAC+NFGHQDF+ELAVEDFN LQCIHRKELKELE+WVIEN+LDK
Sbjct: 433 ALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVIENKLDK 492
Query: 501 LKFARQKLAYCYFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQL 560
LKFARQKLAYCYFSAAATLTSPE DARLSWAKNGVLTTVVDDFFD+GGSEEEL NLIQL
Sbjct: 493 LKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQL 552
Query: 561 VQKWDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLESMRK 620
V+KWDA+G A YCSKEVEIIFLALHSTICEIGE+AL WQGRSV RNVIDIWLALLESMRK
Sbjct: 553 VEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWLALLESMRK 612
Query: 621 EAEWLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQKLFKLMST 680
EAEWLKNKV PS+ EYMENGY SFALGPIVLPTLYFVGPKL E+IGNCEYQKLFKLMST
Sbjct: 613 EAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMST 672
Query: 681 CGRLLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELLRLVL 740
GRLLNDTRTFDRES+EGKLNA+SL +I+ GG LSKEEATEAMK DV+R RRELLRLVL
Sbjct: 673 SGRLLNDTRTFDRESSEGKLNALSLYLIS-AGGKLSKEEATEAMKGDVDRTRRELLRLVL 732
Query: 741 QEKNSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQELMNSAKALFEHPMVLDELLRK 798
QE NSTIPRACKDLFWKMSCVVHLFYRKDDGFTS ELMNSAKALFE PMVLDELL K
Sbjct: 733 QE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALFELPMVLDELLNK 785
BLAST of Sed0013143 vs. NCBI nr
Match:
KAA0062808.1 (ent-kaur-16-ene synthase [Cucumis melo var. makuwa])
HSP 1 Score: 1358.2 bits (3514), Expect = 0.0e+00
Identity = 672/785 (85.61%), Postives = 725/785 (92.36%), Query Frame = 0
Query: 13 FFTISVSSTLSISSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAW 72
F + + +S SSLFPGL+V+ KTK GALR FEDTKERI+KLF VELSVSAYDTAW
Sbjct: 79 FSLLPICFFVSTESSLFPGLDVDTKTKTGALR--FEDTKERIQKLFKNVELSVSAYDTAW 138
Query: 73 VAMVPSPNSLNKPLFPECIDWVLDNQHSDGSWGILHDHQLLMKATLLSTLACVLTLKRWN 132
VAMVPSPNSLN+PLFPECI+WVLD+Q+ DGSWGILHDHQL+MKATLLSTLACVLTLKRWN
Sbjct: 139 VAMVPSPNSLNRPLFPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWN 198
Query: 133 IGHNHITKALDFIKSNIASATDDKQQSPVGFNIIFPGMIEYAKDLNLNLPLAPMNLDALV 192
IGH+H++KAL FIKSNIASATD+ Q+SPVGF+IIFPGMIEYAKDLNLNLPLA MN+DALV
Sbjct: 199 IGHDHMSKALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALV 258
Query: 193 QKKQLELRSCRSNFEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVTFMH 252
QKK+LELRSC SN EGGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SPSTTAV FMH
Sbjct: 259 QKKELELRSCCSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMH 318
Query: 253 GNDDACFNYLSSLLQKFDGSVPSIYPLDIYARLHMVDNLQKLGIDRHFKEEIRSVLDETY 312
NDD CFNYL S+LQKF SVP+IYPLDIYARLHMVD+LQKLGIDRHFK+EIRSVLDETY
Sbjct: 319 RNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETY 378
Query: 313 RCWMQGEEDIFLDVSTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGYLKDFSASLE 372
RCWMQGEE+IFLD STCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGG+LKDF ASLE
Sbjct: 379 RCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLE 438
Query: 373 LFKASQIILHPDETVLENINSWTSRFLNHGLSSDSVCSDRTDRLVKQEAVNAFEFTYSAS 432
LFKASQII++PDE +LENINSWTSRFLNHGLS SV DRT+RLVKQEAVNAFEF Y+++
Sbjct: 439 LFKASQIIIYPDEFILENINSWTSRFLNHGLSGGSVHFDRTERLVKQEAVNAFEFPYNST 498
Query: 433 LERLLNKKAIENYSGDTVRISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERW 492
LERL NK+A+E+YSGD VRISK+TYAC+NFGHQDF+ELAVEDFN LQCIHRKELKELE+W
Sbjct: 499 LERLSNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKW 558
Query: 493 VIENRLDKLKFARQKLAYCYFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEE 552
VIEN+LDKLKFARQKLAYCYFSAAATLTSPE DARLSWAKNGVLTTVVDDFFD+GGSEE
Sbjct: 559 VIENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEE 618
Query: 553 ELTNLIQLVQKWDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWL 612
EL NLIQLV+KWDA+G A YCSKEVEIIFLALHSTICEIGE+AL WQGRSV RNVIDIWL
Sbjct: 619 ELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWL 678
Query: 613 ALLESMRKEAEWLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQ 672
ALLESMRKEAEWLKNKV PS+ EYMENGY SFALGPIVLPTLYFVGPKL E+IGNCEYQ
Sbjct: 679 ALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQ 738
Query: 673 KLFKLMSTCGRLLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMR 732
KLFKLMST GRLLNDTRTFDRES+EGKLNA+SL +I+ GG LSKEEATEAMK DV+R R
Sbjct: 739 KLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYLIS-AGGKLSKEEATEAMKGDVDRTR 798
Query: 733 RELLRLVLQEKNSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQELMNSAKALFEHPMVLD 792
RELLRLVLQE NSTIPRACKDLFWKMSCVVHLFYRKDDGFTS ELMNSAKALFE PMVLD
Sbjct: 799 RELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALFELPMVLD 858
Query: 793 ELLRK 798
ELL K
Sbjct: 859 ELLNK 859
BLAST of Sed0013143 vs. NCBI nr
Match:
XP_016900364.1 (PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X2 [Cucumis melo])
HSP 1 Score: 1354.0 bits (3503), Expect = 0.0e+00
Identity = 672/777 (86.49%), Postives = 722/777 (92.92%), Query Frame = 0
Query: 21 TLSISSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAWVAMVPSPN 80
T S S+SLFPGL+V+ KTK GALR FEDTKERI+KLF VELSVSAYDTAWVAMVPSPN
Sbjct: 13 TPSSSASLFPGLDVDTKTKTGALR--FEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPN 72
Query: 81 SLNKPLFPECIDWVLDNQHSDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGHNHITK 140
SLNKPLFPECI+WVLD+Q+ DGSWGILHDHQL+MKATLLSTLACVLTLKRWNIGH+H++K
Sbjct: 73 SLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSK 132
Query: 141 ALDFIKSNIASATDDKQQSPVGFNIIFPGMIEYAKDLNLNLPLAPMNLDALVQKKQLELR 200
AL FIKSNIASATD+ Q+SPVGF+IIFPGMIEYAKDLNLNLPLA MN+DALVQKK+LEL
Sbjct: 133 ALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALVQKKELEL- 192
Query: 201 SCRSNFEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVTFMHGNDDACFN 260
RSN EGGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SPSTTAV FMH NDD CFN
Sbjct: 193 --RSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFN 252
Query: 261 YLSSLLQKFDGSVPSIYPLDIYARLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWMQGEE 320
YL S+LQKF SVP+IYPLDIYARLHMVD+LQKLGIDRHFK+EIRSVLDETYRCWMQGEE
Sbjct: 253 YLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEE 312
Query: 321 DIFLDVSTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGYLKDFSASLELFKASQII 380
+IFLD STCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGG+LKDF ASLELFKASQII
Sbjct: 313 NIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLELFKASQII 372
Query: 381 LHPDETVLENINSWTSRFLNHGLSSDSVCSDRTDRLVKQEAVNAFEFTYSASLERLLNKK 440
++PDE +LENINSWTSRFLNHGLS SV SDRT+RLVKQEAVNAFEF Y+++LERL NK+
Sbjct: 373 IYPDEFILENINSWTSRFLNHGLSGGSVHSDRTERLVKQEAVNAFEFPYNSTLERLSNKR 432
Query: 441 AIENYSGDTVRISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIENRLDK 500
A+E+YSGD VRISK+TYAC+NFGHQDF+ELAVEDFN LQCIHRKELKELE+WVIEN+LDK
Sbjct: 433 ALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVIENKLDK 492
Query: 501 LKFARQKLAYCYFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQL 560
LKFARQKLAYCYFSAAATLTSPE DARLSWAKNGVLTTVVDDFFD+GGSEEEL NLIQL
Sbjct: 493 LKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQL 552
Query: 561 VQKWDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLESMRK 620
V+KWDA+G A YCSKEVEIIFLALHSTICEIGE+AL WQGRSV RNVIDIWLALLESMRK
Sbjct: 553 VEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWLALLESMRK 612
Query: 621 EAEWLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQKLFKLMST 680
EAEWLKNKV PS+ EYMENGY SFALGPIVLPTLYFVGPKL E+IGNCEYQKLFKLMST
Sbjct: 613 EAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMST 672
Query: 681 CGRLLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELLRLVL 740
GRLLNDTRTFDRES+EGKLNA+SL +I+ GG LSKEEATEAMK DV+R RRELLRLVL
Sbjct: 673 SGRLLNDTRTFDRESSEGKLNALSLYLIS-AGGKLSKEEATEAMKGDVDRTRRELLRLVL 732
Query: 741 QEKNSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQELMNSAKALFEHPMVLDELLRK 798
QE NSTIPRACKDLFWKMSCVVHLFYRKDDGFTS ELMNSAKALFE PMVLDELL K
Sbjct: 733 QE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALFELPMVLDELLNK 782
BLAST of Sed0013143 vs. NCBI nr
Match:
XP_038888074.1 (ent-kaur-16-ene synthase, chloroplastic isoform X3 [Benincasa hispida])
HSP 1 Score: 1353.2 bits (3501), Expect = 0.0e+00
Identity = 668/777 (85.97%), Postives = 719/777 (92.54%), Query Frame = 0
Query: 21 TLSISSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAWVAMVPSPN 80
T S S+SLFP L+V+ KTK GALR E+TKERIKKLF+KVELSVSAYDTAWVAMVPSPN
Sbjct: 13 TASSSASLFPELDVDTKTKTGALR--LEETKERIKKLFDKVELSVSAYDTAWVAMVPSPN 72
Query: 81 SLNKPLFPECIDWVLDNQHSDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGHNHITK 140
SLNKPLFPECI+WVLDNQH DGSWGILHDHQLLMKATLLSTLACVLTLKRWNIG +H++K
Sbjct: 73 SLNKPLFPECINWVLDNQHPDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGQDHMSK 132
Query: 141 ALDFIKSNIASATDDKQQSPVGFNIIFPGMIEYAKDLNLNLPLAPMNLDALVQKKQLELR 200
AL FIKSNIASATD+KQ+SPVGF+IIFPGMIEYAKDLNLNLPLAPM +DALVQKK+LELR
Sbjct: 133 ALSFIKSNIASATDEKQRSPVGFDIIFPGMIEYAKDLNLNLPLAPMKVDALVQKKELELR 192
Query: 201 SCRSNFEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVTFMHGNDDACFN 260
SCRSN EGGKAYLAYVSEGI LQDWEMVMQYQRKNGSLFNSPSTTAV FMH NDD CFN
Sbjct: 193 SCRSNSEGGKAYLAYVSEGIENLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFN 252
Query: 261 YLSSLLQKFDGSVPSIYPLDIYARLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWMQGEE 320
YL S+LQKFD SVP+IYPLDIYARLHMVD+LQK GI RHFKEEIRSVLDETYRCWMQGEE
Sbjct: 253 YLRSVLQKFDDSVPTIYPLDIYARLHMVDSLQKFGIGRHFKEEIRSVLDETYRCWMQGEE 312
Query: 321 DIFLDVSTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGYLKDFSASLELFKASQII 380
+IFLD STCAMAFR+LRVEGYDVSSD+LTQFSE LFPNCLGG+LKDF AS+ELFKASQII
Sbjct: 313 NIFLDASTCAMAFRLLRVEGYDVSSDKLTQFSEVLFPNCLGGHLKDFGASMELFKASQII 372
Query: 381 LHPDETVLENINSWTSRFLNHGLSSDSVCSDRTDRLVKQEAVNAFEFTYSASLERLLNKK 440
++PDE++LEN+NSWTSRFLNHGLS V SDRTDRLVKQEAVNA EF Y+A+LERLLNK+
Sbjct: 373 IYPDESILENVNSWTSRFLNHGLSGGLVRSDRTDRLVKQEAVNALEFPYNATLERLLNKR 432
Query: 441 AIENYSGDTVRISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIENRLDK 500
A+E+YSGD VRISK+TYAC+NFGHQDF+ELAVEDFN LQCIHRKELKELE+WV+EN+LDK
Sbjct: 433 ALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDK 492
Query: 501 LKFARQKLAYCYFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQL 560
LKFARQKLAYCYFSAAATLTSPE DARLSWAKNGVLTTVVDDFFD+GGSEEEL NLIQL
Sbjct: 493 LKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELLNLIQL 552
Query: 561 VQKWDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLESMRK 620
V+KWDANG YCSKEVEIIFLALHST+C+IG RAL+WQGRSV RNVIDIWLALLESMRK
Sbjct: 553 VEKWDANGETGYCSKEVEIIFLALHSTVCDIGRRALSWQGRSVTRNVIDIWLALLESMRK 612
Query: 621 EAEWLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQKLFKLMST 680
EAEWLKNKV PS+ EYMENGY SFALGPIVLPTLYFVGPKL EVIGNCEY+KLFKLMST
Sbjct: 613 EAEWLKNKVVPSMDEYMENGYVSFALGPIVLPTLYFVGPKLPEEVIGNCEYKKLFKLMST 672
Query: 681 CGRLLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELLRLVL 740
CGRLLNDTRTFDRES+EGKLNA+SL +IN GG L+KEEATEAMK DVER RRELLRLVL
Sbjct: 673 CGRLLNDTRTFDRESSEGKLNAMSLCIIN-AGGKLTKEEATEAMKGDVERTRRELLRLVL 732
Query: 741 QEKNSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQELMNSAKALFEHPMVLDELLRK 798
QE N+TIPRACKDLF KMSCVVHLFYRKDDGFTS ELM SAKALFE PMVLD+LL K
Sbjct: 733 QE-NTTIPRACKDLFRKMSCVVHLFYRKDDGFTSHELMKSAKALFEQPMVLDQLLNK 785
BLAST of Sed0013143 vs. NCBI nr
Match:
XP_038888071.1 (ent-kaur-16-ene synthase, chloroplastic isoform X1 [Benincasa hispida] >XP_038888072.1 ent-kaur-16-ene synthase, chloroplastic isoform X1 [Benincasa hispida])
HSP 1 Score: 1353.2 bits (3501), Expect = 0.0e+00
Identity = 668/777 (85.97%), Postives = 719/777 (92.54%), Query Frame = 0
Query: 21 TLSISSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAWVAMVPSPN 80
T S S+SLFP L+V+ KTK GALR E+TKERIKKLF+KVELSVSAYDTAWVAMVPSPN
Sbjct: 68 TASSSASLFPELDVDTKTKTGALR--LEETKERIKKLFDKVELSVSAYDTAWVAMVPSPN 127
Query: 81 SLNKPLFPECIDWVLDNQHSDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGHNHITK 140
SLNKPLFPECI+WVLDNQH DGSWGILHDHQLLMKATLLSTLACVLTLKRWNIG +H++K
Sbjct: 128 SLNKPLFPECINWVLDNQHPDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGQDHMSK 187
Query: 141 ALDFIKSNIASATDDKQQSPVGFNIIFPGMIEYAKDLNLNLPLAPMNLDALVQKKQLELR 200
AL FIKSNIASATD+KQ+SPVGF+IIFPGMIEYAKDLNLNLPLAPM +DALVQKK+LELR
Sbjct: 188 ALSFIKSNIASATDEKQRSPVGFDIIFPGMIEYAKDLNLNLPLAPMKVDALVQKKELELR 247
Query: 201 SCRSNFEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVTFMHGNDDACFN 260
SCRSN EGGKAYLAYVSEGI LQDWEMVMQYQRKNGSLFNSPSTTAV FMH NDD CFN
Sbjct: 248 SCRSNSEGGKAYLAYVSEGIENLQDWEMVMQYQRKNGSLFNSPSTTAVAFMHRNDDGCFN 307
Query: 261 YLSSLLQKFDGSVPSIYPLDIYARLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWMQGEE 320
YL S+LQKFD SVP+IYPLDIYARLHMVD+LQK GI RHFKEEIRSVLDETYRCWMQGEE
Sbjct: 308 YLRSVLQKFDDSVPTIYPLDIYARLHMVDSLQKFGIGRHFKEEIRSVLDETYRCWMQGEE 367
Query: 321 DIFLDVSTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGYLKDFSASLELFKASQII 380
+IFLD STCAMAFR+LRVEGYDVSSD+LTQFSE LFPNCLGG+LKDF AS+ELFKASQII
Sbjct: 368 NIFLDASTCAMAFRLLRVEGYDVSSDKLTQFSEVLFPNCLGGHLKDFGASMELFKASQII 427
Query: 381 LHPDETVLENINSWTSRFLNHGLSSDSVCSDRTDRLVKQEAVNAFEFTYSASLERLLNKK 440
++PDE++LEN+NSWTSRFLNHGLS V SDRTDRLVKQEAVNA EF Y+A+LERLLNK+
Sbjct: 428 IYPDESILENVNSWTSRFLNHGLSGGLVRSDRTDRLVKQEAVNALEFPYNATLERLLNKR 487
Query: 441 AIENYSGDTVRISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIENRLDK 500
A+E+YSGD VRISK+TYAC+NFGHQDF+ELAVEDFN LQCIHRKELKELE+WV+EN+LDK
Sbjct: 488 ALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVVENKLDK 547
Query: 501 LKFARQKLAYCYFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQL 560
LKFARQKLAYCYFSAAATLTSPE DARLSWAKNGVLTTVVDDFFD+GGSEEEL NLIQL
Sbjct: 548 LKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELLNLIQL 607
Query: 561 VQKWDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLESMRK 620
V+KWDANG YCSKEVEIIFLALHST+C+IG RAL+WQGRSV RNVIDIWLALLESMRK
Sbjct: 608 VEKWDANGETGYCSKEVEIIFLALHSTVCDIGRRALSWQGRSVTRNVIDIWLALLESMRK 667
Query: 621 EAEWLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQKLFKLMST 680
EAEWLKNKV PS+ EYMENGY SFALGPIVLPTLYFVGPKL EVIGNCEY+KLFKLMST
Sbjct: 668 EAEWLKNKVVPSMDEYMENGYVSFALGPIVLPTLYFVGPKLPEEVIGNCEYKKLFKLMST 727
Query: 681 CGRLLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELLRLVL 740
CGRLLNDTRTFDRES+EGKLNA+SL +IN GG L+KEEATEAMK DVER RRELLRLVL
Sbjct: 728 CGRLLNDTRTFDRESSEGKLNAMSLCIIN-AGGKLTKEEATEAMKGDVERTRRELLRLVL 787
Query: 741 QEKNSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQELMNSAKALFEHPMVLDELLRK 798
QE N+TIPRACKDLF KMSCVVHLFYRKDDGFTS ELM SAKALFE PMVLD+LL K
Sbjct: 788 QE-NTTIPRACKDLFRKMSCVVHLFYRKDDGFTSHELMKSAKALFEQPMVLDQLLNK 840
BLAST of Sed0013143 vs. ExPASy Swiss-Prot
Match:
Q39548 (Ent-kaur-16-ene synthase, chloroplastic OS=Cucurbita maxima OX=3661 PE=1 SV=1)
HSP 1 Score: 1260.0 bits (3259), Expect = 0.0e+00
Identity = 616/781 (78.87%), Postives = 696/781 (89.12%), Query Frame = 0
Query: 17 SVSSTLSISSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAWVAMV 76
+ SS+ S S+SLFPG++V+ TK GAL FE+TKERIKKLF+KVELSVSAYDTAWVAMV
Sbjct: 13 AASSSSSSSASLFPGVDVDTTTKTGAL--HFEETKERIKKLFDKVELSVSAYDTAWVAMV 72
Query: 77 PSPNSLNKPLFPECIDWVLDNQHSDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGHN 136
PSPNSLN+PLFPECI+WVLD+QH+DGSWG+LH+ QLLMKA LLSTLACVLTLKRWNIGH+
Sbjct: 73 PSPNSLNQPLFPECINWVLDSQHADGSWGLLHNDQLLMKANLLSTLACVLTLKRWNIGHD 132
Query: 137 HITKALDFIKSNIASATDDKQQSPVGFNIIFPGMIEYAKDLNLNLPLAPMNLDALVQKKQ 196
H++KALDFIKSNIASATD+ Q+SPVGF+IIFPGMIEYAKDLNLNLPLAP N+DALV+KK+
Sbjct: 133 HMSKALDFIKSNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLAPTNVDALVRKKE 192
Query: 197 LELRSCRSNFEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVTFMHGNDD 256
LELRSCRSN EGGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLFNSPSTTA FMH NDD
Sbjct: 193 LELRSCRSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFNSPSTTAAAFMHRNDD 252
Query: 257 ACFNYLSSLLQKFDGSVPSIYPLDIYARLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWM 316
CF+YL SLLQKFDGSVP+IYPLDIYARLHMVD+LQK GI RHFKEEIRSVLDETYRCWM
Sbjct: 253 GCFDYLRSLLQKFDGSVPTIYPLDIYARLHMVDSLQKFGIARHFKEEIRSVLDETYRCWM 312
Query: 317 QGEEDIFLDVSTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGYLKDFSASLELFKA 376
QGEE+IFLD STCAMAFRMLRVEGYDVSSDQLTQFSED+FPNCLGGYLKDF ASLEL+KA
Sbjct: 313 QGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDIFPNCLGGYLKDFGASLELYKA 372
Query: 377 SQIILHPDETVLENINSWTSRFLNHGLSSDSVCSDRTDRLVKQEAVNAFEFTYSASLERL 436
SQII HPDE+VLENINSWTSRFL HGLSSDSV SDRTD +VKQEAVNA EF Y+A+LERL
Sbjct: 373 SQIITHPDESVLENINSWTSRFLKHGLSSDSVWSDRTDSVVKQEAVNALEFPYNATLERL 432
Query: 437 LNKKAIENYSGDTVRISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIEN 496
++K+A+E+YSGD VRISKS YAC+NFGHQDF+ELAVEDFN LQ IH KEL+EL+RWV+EN
Sbjct: 433 ISKRAMESYSGDIVRISKSPYACLNFGHQDFLELAVEDFNTLQRIHLKELEELQRWVVEN 492
Query: 497 RLDKLKFARQKLAYCYFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEEELTN 556
+LD+LKF R L YCYF+AAATLT PE DAR++WA+NGVLTTVVDDF+D GGSEEEL N
Sbjct: 493 KLDELKFFRLHLGYCYFAAAATLTDPELHDARIAWAQNGVLTTVVDDFYDGGGSEEELDN 552
Query: 557 LIQLVQKWDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLE 616
LI+LV+KWD +G YCSK+VEI+FLALHST+CEIG RAL WQGRSVMRNVID WLALL+
Sbjct: 553 LIELVEKWDPDGEVGYCSKDVEIVFLALHSTVCEIGRRALVWQGRSVMRNVIDGWLALLK 612
Query: 617 SMRKEAEWLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQKLFK 676
MRKEAEW NKV PS+ EYME + SFALGPI+LP L+FVGPKLS E+IG+CEYQKL+K
Sbjct: 613 VMRKEAEWSTNKVVPSMGEYMEQAHVSFALGPIILPMLFFVGPKLSEEMIGSCEYQKLYK 672
Query: 677 LMSTCGRLLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELL 736
LMST GRL ND R++DRE EGKLN +SL MI+ GGGN++KEEA EA+K D ER RELL
Sbjct: 673 LMSTAGRLKNDIRSYDRECKEGKLNILSLWMID-GGGNVTKEEAIEAIKGDFERAIRELL 732
Query: 737 RLVLQEKNSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQELMNSAKALFEHPMVLDELLR 796
LVLQE N+TIPRACKDLFWK+ +V+LFY +DDG+TS LMN+ KA+FE PM LD LL
Sbjct: 733 GLVLQE-NTTIPRACKDLFWKLMSIVNLFYMEDDGYTSNRLMNTVKAMFEQPMDLDALLN 789
Query: 797 K 798
K
Sbjct: 793 K 789
BLAST of Sed0013143 vs. ExPASy Swiss-Prot
Match:
A0A2K9RFY0 (Ent-kaurene synthase TSP4, chloroplastic OS=Vitex agnus-castus OX=54477 GN=TPS4 PE=1 SV=1)
HSP 1 Score: 855.9 bits (2210), Expect = 3.6e-247
Identity = 426/768 (55.47%), Postives = 555/768 (72.27%), Query Frame = 0
Query: 24 ISSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAWVAMVPSPNSLN 83
+S+SL G V + T FE+TKERI L +K ELSVS YDTAWVAMVPSPNS
Sbjct: 24 VSASLDTG--VRRVTSAKIASTCFEETKERIADLIHKAELSVSTYDTAWVAMVPSPNSSQ 83
Query: 84 KPLFPECIDWVLDNQHSDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGHNHITKALD 143
+P FP+C+ W+L NQ DGSW H H LL K L STLACVL LK+W +G I + +
Sbjct: 84 EPCFPDCLSWLLQNQCCDGSWACPHHHPLLKKDVLCSTLACVLALKKWGVGEEKINRGVH 143
Query: 144 FIKSNIASATDDKQQSPVGFNIIFPGMIEYAKDLNLNLPLAPMNLDALVQKKQLELRSCR 203
FI+ N ASA + Q SP+GF+IIFP M++YA+DL LNL L P L+ L+ K+ LEL+ +
Sbjct: 144 FIEHNFASAMEKCQISPMGFDIIFPAMLDYARDLLLNLRLEPTMLNDLIYKRGLELKRNQ 203
Query: 204 SNFEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVTFMHGNDDACFNYLS 263
++ +AYLAYV+EG+GKLQD VM++QR+NGSLFNSPSTTA F+ + C YL
Sbjct: 204 NHSAEREAYLAYVAEGMGKLQDLGSVMKHQRRNGSLFNSPSTTAAAFIAFPNSRCLTYLR 263
Query: 264 SLLQKFDGSVPSIYPLDIYARLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWMQGEEDIF 323
S L+KF +VP++YPLDIY +L VDNL++LGI R+F++EI+ VLDETYRCW+QG E+IF
Sbjct: 264 SALKKFGSAVPAVYPLDIYLQLCTVDNLERLGISRYFQKEIQGVLDETYRCWLQGNEEIF 323
Query: 324 LDVSTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGYLKDFSASLELFKASQIILHP 383
+D TCA+AFR+LR GY+V+SD +T+ E+ F + G +KD + +L L++AS+ IL+P
Sbjct: 324 MDAPTCALAFRVLRKNGYNVTSDPITKLLEECFSSSFCGNIKDINTTLGLYRASEFILYP 383
Query: 384 DETVLENINSWTSRFLNHGLSSDSVCSDRTDRLVKQEAVNAFEFTYSASLERLLNKKAIE 443
DE LE N L LSSD + S + R + E +A E+ + A L+R++N++ IE
Sbjct: 384 DERDLEKQNLMLKNLLEQELSSDFIHSSQLGRNIDAEVKHALEYPFYADLDRIVNRRNIE 443
Query: 444 NYSGDTVRISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIENRLDKLKF 503
+Y+ D RI K++Y NFG++DF+ L+V+D+N Q IHR+EL+ELERWVIENRLD+L+F
Sbjct: 444 HYNFDNTRILKTSYCSPNFGNKDFLFLSVKDYNECQAIHREELRELERWVIENRLDELRF 503
Query: 504 ARQKLAYCYFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQLVQK 563
ARQK AYCYFSAAATL +PE +AR+SWAKNGVLTTVVDDFFD+GGS EEL NLIQLV+
Sbjct: 504 ARQKCAYCYFSAAATLFAPELSNARMSWAKNGVLTTVVDDFFDLGGSVEELKNLIQLVEL 563
Query: 564 WDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLESMRKEAE 623
WD + + S+ V+IIF AL TIC+IG++ Q RS+ ++IDIWL LL SM KE E
Sbjct: 564 WDVDVSTECSSQNVQIIFSALKCTICDIGDKGSKLQERSITNHIIDIWLDLLYSMMKETE 623
Query: 624 WLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQKLFKLMSTCGR 683
W ++K P++ EY+ N Y SFALGPIVLP LY VGPKLS E++ + EY L+KLMSTCGR
Sbjct: 624 WARDKYIPTMDEYISNAYVSFALGPIVLPALYLVGPKLSEEMVHHSEYHNLYKLMSTCGR 683
Query: 684 LLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELLRLVLQEK 743
LLND R +RE EGKLNA+ L +IN GG ++KE A MK +E RRELLRLVL+ K
Sbjct: 684 LLNDIRGCERELKEGKLNAIPLYIIN-NGGEITKEAAASEMKSLIETHRRELLRLVLEGK 743
Query: 744 NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQELMNSAKALFEHPMVL 792
NS +P++CK+LFW MS V+HLFY KDDGFTSQ+L+ KA+ P+VL
Sbjct: 744 NSVLPKSCKELFWHMSKVLHLFYSKDDGFTSQDLIKVVKAVIYEPIVL 788
BLAST of Sed0013143 vs. ExPASy Swiss-Prot
Match:
A0A1Z3GC64 (Ent-kaurene synthase 5, chloroplastic OS=Isodon rubescens OX=587669 GN=KSL5 PE=1 SV=1)
HSP 1 Score: 847.0 bits (2187), Expect = 1.7e-244
Identity = 422/771 (54.73%), Postives = 551/771 (71.47%), Query Frame = 0
Query: 25 SSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAWVAMVPSPNSLNK 84
S+SL GL+ +N ++ FE+TK RI KLF+K ELSVS YDTAWVAMVPSP S +
Sbjct: 24 SASLDIGLQRATSPQNASVATCFEETKGRIAKLFHKNELSVSTYDTAWVAMVPSPTSSEE 83
Query: 85 PLFPECIDWVLDNQHSDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGHNHITKALDF 144
P FP C++W+L+NQ DGSW H H +L K L STLAC+L LK+W +G I + L F
Sbjct: 84 PCFPACLNWLLENQCHDGSWARPHHHHMLKKDVLSSTLACILALKKWGVGEEQINRGLHF 143
Query: 145 IKSNIASATDDKQQSPVGFNIIFPGMIEYAKDLNLNLPLAPMNLDALVQKKQLELRSC-R 204
++ N ASAT+ Q +P+GF+IIFP M++ A+ L+LNL L P L+ L+ K+ LEL+ C +
Sbjct: 144 VELNFASATEKGQITPMGFDIIFPAMLDNARGLSLNLQLEPTTLNDLIYKRDLELKRCNQ 203
Query: 205 SNFEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVTFMHGNDDACFNYLS 264
SN + Y A+++EG+GKLQDWE VM+YQRKNGSLFN PSTTA F + C NYL
Sbjct: 204 SNSAEKEVYWAHIAEGMGKLQDWESVMKYQRKNGSLFNCPSTTAAAFTALRNSDCLNYLR 263
Query: 265 SLLQKFDGSVPSIYPLDIYARLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWMQGEEDIF 324
L+KF +VP++YPLDIY++L VDNL++LGI R+F EI+SVLDETYR W+QG+E+IF
Sbjct: 264 LALEKFGSAVPAVYPLDIYSQLCTVDNLERLGISRYFLTEIQSVLDETYRSWLQGDEEIF 323
Query: 325 LDVSTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGYLKDFSASLELFKASQIILHP 384
+D STCA+AFR LR+ GY+V+SD +T+ ++ F + G + D + +LE+++AS++IL+P
Sbjct: 324 MDASTCALAFRTLRMNGYNVTSDPITKILQECFSSSFRGNMTDINTTLEIYRASELILYP 383
Query: 385 DETVLENINSWTSRFLNHGLSSDS-VCSDRTDRLVKQEAVNAFEFTYSASLERLLNKKAI 444
+E L+ N FL LSS+ + S + R + E A E+ + A ++R+ +K I
Sbjct: 384 EERDLDQHNLRLKTFLEQELSSNGFIQSCQLGRNINAEVNQAIEYPFYAIMDRMAKRKNI 443
Query: 445 ENYSGDTVRISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIENRLDKLK 504
ENY+ D RI K++Y NFG++DF+ L+VEDFN Q IHR+EL+ELERWVIENRLD+LK
Sbjct: 444 ENYNIDNTRILKTSYRSPNFGNKDFLSLSVEDFNRCQVIHREELRELERWVIENRLDELK 503
Query: 505 FARQKLAYCYFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQLVQ 564
FAR K AYCYFSAAAT+ SPE DAR+SWAKNGVLTTVVDDFFD+GGS EEL NLIQLV+
Sbjct: 504 FARSKAAYCYFSAAATIFSPELSDARMSWAKNGVLTTVVDDFFDVGGSVEELKNLIQLVE 563
Query: 565 KWDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLESMRKEA 624
WD + CS V+IIF AL TICEI ++ QGRS+ ++I IWL LL SM KE
Sbjct: 564 LWDVDVSTQCCSPNVQIIFSALKHTICEIADKGFKLQGRSITDHIISIWLDLLYSMMKET 623
Query: 625 EWLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQKLFKLMSTCG 684
E +K P++ EYM N Y SFALGPIVLP LY VGPKLS E++ + EY LFKLMSTCG
Sbjct: 624 ELGIDKSFPTMDEYMSNAYVSFALGPIVLPALYLVGPKLSEEMVNHSEYHTLFKLMSTCG 683
Query: 685 RLLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELLRLVLQE 744
RLLND R ++RE +GK++AVSL ++N GG ++ E A M+ +ER RRELLRLVL+E
Sbjct: 684 RLLNDIRGYERELKDGKISAVSLYIMN-NGGEITTEAAISEMRSWIERDRRELLRLVLEE 743
Query: 745 KNSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQELMNSAKALFEHPMVLDE 794
S +P+ACK LFW M VVHLFYRKDDGFTS +L A+ P+VL++
Sbjct: 744 NKSVLPKACKKLFWHMCTVVHLFYRKDDGFTSLDLHGVVNAIINEPIVLNQ 793
BLAST of Sed0013143 vs. ExPASy Swiss-Prot
Match:
A0A0U4CDK4 (Ent-kaur-16-ene synthase, chloroplastic OS=Salvia miltiorrhiza OX=226208 GN=KS PE=1 SV=1)
HSP 1 Score: 837.4 bits (2162), Expect = 1.3e-241
Identity = 411/770 (53.38%), Postives = 542/770 (70.39%), Query Frame = 0
Query: 25 SSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAWVAMVPSPNSLNK 84
S+SL GL K ++ FE T+ RI KLF+K ELSVS YDTAWVAMVPSP SL +
Sbjct: 27 SASLKSGLHSATSAKIASMPTCFEQTRGRIAKLFHKDELSVSTYDTAWVAMVPSPTSLEE 86
Query: 85 PLFPECIDWVLDNQHSDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGHNHITKALDF 144
P FP+C++W+L+NQ DGSW H H LL K L STLAC+L LK+W +G I + L F
Sbjct: 87 PCFPDCLNWLLENQCHDGSWARPHHHPLLKKDVLSSTLACILALKKWGVGEEQIKRGLHF 146
Query: 145 IKSNIASATDDKQQSPVGFNIIFPGMIEYAKDLNLNLPLAPMNLDALVQKKQLELRSCRS 204
++ N ASATD Q +P+GF+IIFP M++YA+ +LNL L P + L+ K+ +EL+
Sbjct: 147 LELNFASATDKCQITPMGFDIIFPAMLDYARGFSLNLRLDPTTFNDLMHKRDVELKRSNR 206
Query: 205 NFEG-GKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVTFMHGNDDACFNYLS 264
N+ + Y AY++EG+G+LQ+WE VM+YQR+NGSLFN PSTTA F+ + C NYL
Sbjct: 207 NYSSETETYWAYIAEGMGELQNWESVMKYQRRNGSLFNCPSTTAAAFIALRNSDCLNYLH 266
Query: 265 SLLQKFDGSVPSIYPLDIYARLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWMQGEEDIF 324
L+KF +V ++YPLDIY++L VDNL++LGI ++F EI++VLDETYRCWMQG E+IF
Sbjct: 267 LALKKFGNAVSAVYPLDIYSQLCTVDNLERLGISQYFSTEIQNVLDETYRCWMQGNEEIF 326
Query: 325 LDVSTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGYLKDFSASLELFKASQIILHP 384
+D STCA+AFR LR+ GYDV+SD +TQ ++ F + G + D + +LEL++AS+++L+P
Sbjct: 327 MDASTCALAFRTLRLNGYDVTSDPVTQILQECFSSSFRGNMTDINTTLELYRASELVLYP 386
Query: 385 DETVLENINSWTSRFLNHGLSSDSVCSDRTDRLVKQEAVNAFEFTYSASLERLLNKKAIE 444
DE LE N L LSS + S + R + E A E+ + A ++R+ +K+IE
Sbjct: 387 DERDLEKQNLRLKLLLEQELSSGLIQSCQLGRSINVEVNQAIEYPFYAIMDRVAKRKSIE 446
Query: 445 NYSGDTVRISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIENRLDKLKF 504
+Y+ D RI K++Y NF ++DF+ L++EDFN Q HR+EL ELERWV+ENRLD+LKF
Sbjct: 447 HYNFDNTRILKTSYCSPNFSNEDFLFLSIEDFNRCQAAHREELGELERWVVENRLDELKF 506
Query: 505 ARQKLAYCYFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQLVQK 564
AR K AYCYFSAAAT +PE DARLSWAKNGVLTTV+DDFFD+GGS EEL NLIQLV+
Sbjct: 507 ARSKSAYCYFSAAATFFAPELLDARLSWAKNGVLTTVIDDFFDVGGSVEELKNLIQLVEL 566
Query: 565 WDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLESMRKEAE 624
WD + + S V+IIF AL TICEIG++A QGR + ++I IWL LL SM +E E
Sbjct: 567 WDVDISTECYSHNVQIIFSALRRTICEIGDKAFKLQGRCITNHIIAIWLDLLNSMMRETE 626
Query: 625 WLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQKLFKLMSTCGR 684
W ++ P+I EYM N + SFALGPIVLP LY VGPKLS E++ + EY LFKLMSTCGR
Sbjct: 627 WARDNFVPTIDEYMSNAHVSFALGPIVLPALYLVGPKLSEEMVNHSEYHNLFKLMSTCGR 686
Query: 685 LLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELLRLVLQEK 744
LLND ++RE +GKLNA+SL +IN GG +SKE A MK +E RRELLRLVL+ K
Sbjct: 687 LLNDIHGYERELKDGKLNALSLYIIN-HGGEVSKEAAIWEMKSWIETQRRELLRLVLEGK 746
Query: 745 NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQELMNSAKALFEHPMVLDE 794
S +P+ C++LFW M VVHLFY K DGFTSQ+L+ + P++L++
Sbjct: 747 KSVLPKPCRELFWHMCSVVHLFYSKGDGFTSQDLIQLVNTIIHQPILLND 795
BLAST of Sed0013143 vs. ExPASy Swiss-Prot
Match:
A0A3G1QTS7 (Ent-kaurene synthase 1, chloroplastic OS=Isodon eriocalyx OX=662907 GN=KS1 PE=1 SV=1)
HSP 1 Score: 834.3 bits (2154), Expect = 1.1e-240
Identity = 418/771 (54.22%), Postives = 546/771 (70.82%), Query Frame = 0
Query: 25 SSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAWVAMVPSPNSLNK 84
S+SL GL+ +N ++ FE+TK RI KLF+K ELSVS YDTAWVAMVPSP S +
Sbjct: 24 SASLDIGLQRATSPQNASVPTCFEETKGRIAKLFHKNELSVSTYDTAWVAMVPSPTSSEE 83
Query: 85 PLFPECIDWVLDNQHSDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGHNHITKALDF 144
P FP C++W+L+NQ DGSW H H +L K L STLAC+L LK+W +G I + L F
Sbjct: 84 PCFPACLNWLLENQCHDGSWARPHHHHMLKKDVLSSTLACILALKKWGVGEEQINRGLHF 143
Query: 145 IKSNIASATDDKQQSPVGFNIIFPGMIEYAKDLNLNLPLAPMNLDALVQKKQLELRSC-R 204
++ N ASAT+ Q +P+GF+IIFP M++ A+ L+LNL L P L+ L+ K+ LEL+ C +
Sbjct: 144 VELNFASATEKGQITPMGFDIIFPAMLDNARGLSLNLQLEPTMLNDLIYKRDLELKRCNQ 203
Query: 205 SNFEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVTFMHGNDDACFNYLS 264
SN + Y A+++EG+GKL DWE VM+YQRKNGSLFN PSTTA F + C NYL
Sbjct: 204 SNSAEKEVYWAHIAEGMGKLHDWESVMKYQRKNGSLFNCPSTTAAAFTALRNSDCLNYLC 263
Query: 265 SLLQKFDGSVPSIYPLDIYARLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWMQGEEDIF 324
L+KF +VP++YPLDIY++L V NL++LGI R+F EI+SVLDETYR W+QG+E+IF
Sbjct: 264 LALEKFGSAVPAVYPLDIYSQLCTVGNLERLGISRYFLTEIQSVLDETYRSWLQGDEEIF 323
Query: 325 LDVSTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGYLKDFSASLELFKASQIILHP 384
+D STCA+AFR LR+ GY+V+SD +T+ ++ F + G + D + +LE+++AS++IL+P
Sbjct: 324 MDASTCALAFRTLRMNGYNVTSDPITKILQECFSSSFRGNMTDNNTTLEIYRASELILYP 383
Query: 385 DETVLENINSWTSRFLNHGLSSDS-VCSDRTDRLVKQEAVNAFEFTYSASLERLLNKKAI 444
+E L N FL LSS+ + S + R + E A E+ + A ++R+ +K I
Sbjct: 384 EERDLVQHNLRLKTFLEQELSSNGFIQSCQLGRNINAEVNQAIEYPFYAIMDRMAKRKNI 443
Query: 445 ENYSGDTVRISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIENRLDKLK 504
ENY+ D RI K++Y NFG++DF+ L+VEDFN Q IHR+EL+ELERWVIENRLD+LK
Sbjct: 444 ENYNIDNTRILKTSYRSPNFGNKDFLSLSVEDFNRCQVIHREELRELERWVIENRLDELK 503
Query: 505 FARQKLAYCYFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQLVQ 564
FAR K AYCYFSAAAT+ SPE DAR+SWAKNGVLTTVVDDFFD+GGS EEL NLIQLV+
Sbjct: 504 FARSKAAYCYFSAAATIFSPELSDARMSWAKNGVLTTVVDDFFDVGGSVEELKNLIQLVE 563
Query: 565 KWDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLESMRKEA 624
WD + CS V+IIF AL TICEI ++ QGRS+ ++I IWL LL SM KE
Sbjct: 564 LWDVDVSTQCCSPNVQIIFSALKHTICEIADKGFKLQGRSITDHIISIWLDLLYSMMKET 623
Query: 625 EWLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQKLFKLMSTCG 684
E +K P++ EYM N Y SFALGPIVLP LY VGPKLS E++ + EY LFKLMSTCG
Sbjct: 624 ELGIDKSFPTMDEYMSNAYVSFALGPIVLPALYLVGPKLSEEMVNHSEYHTLFKLMSTCG 683
Query: 685 RLLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELLRLVLQE 744
RLLND R ++RE +GK++AVSL ++N GG ++ E A M+ +ER RRELLRLVL+E
Sbjct: 684 RLLNDIRGYERELKDGKISAVSLYIMN-NGGEITTEAAISEMRSWIERDRRELLRLVLEE 743
Query: 745 KNSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQELMNSAKALFEHPMVLDE 794
S +P+ACK LFW M VVH+FY KDDGFTS L A+ P+VL++
Sbjct: 744 NKSVLPKACKKLFWHMCTVVHVFYSKDDGFTSLNLHGVVNAIINEPIVLNQ 793
BLAST of Sed0013143 vs. ExPASy TrEMBL
Match:
A0A1S3BG06 (ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489622 PE=4 SV=1)
HSP 1 Score: 1363.2 bits (3527), Expect = 0.0e+00
Identity = 674/777 (86.74%), Postives = 724/777 (93.18%), Query Frame = 0
Query: 21 TLSISSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAWVAMVPSPN 80
T S S+SLFPGL+V+ KTK GALR FEDTKERI+KLF VELSVSAYDTAWVAMVPSPN
Sbjct: 13 TPSSSASLFPGLDVDTKTKTGALR--FEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPN 72
Query: 81 SLNKPLFPECIDWVLDNQHSDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGHNHITK 140
SLNKPLFPECI+WVLD+Q+ DGSWGILHDHQL+MKATLLSTLACVLTLKRWNIGH+H++K
Sbjct: 73 SLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSK 132
Query: 141 ALDFIKSNIASATDDKQQSPVGFNIIFPGMIEYAKDLNLNLPLAPMNLDALVQKKQLELR 200
AL FIKSNIASATD+ Q+SPVGF+IIFPGMIEYAKDLNLNLPLA MN+DALVQKK+LELR
Sbjct: 133 ALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALVQKKELELR 192
Query: 201 SCRSNFEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVTFMHGNDDACFN 260
SC SN EGGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SPSTTAV FMH NDD CFN
Sbjct: 193 SCCSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFN 252
Query: 261 YLSSLLQKFDGSVPSIYPLDIYARLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWMQGEE 320
YL S+LQKF SVP+IYPLDIYARLHMVD+LQKLGIDRHFK+EIRSVLDETYRCWMQGEE
Sbjct: 253 YLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEE 312
Query: 321 DIFLDVSTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGYLKDFSASLELFKASQII 380
+IFLD STCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGG+LKDF ASLELFKASQII
Sbjct: 313 NIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLELFKASQII 372
Query: 381 LHPDETVLENINSWTSRFLNHGLSSDSVCSDRTDRLVKQEAVNAFEFTYSASLERLLNKK 440
++PDE +LENINSWTSRFLNHGLS SV SDRT+RLVKQEAVNAFEF Y+++LERL NK+
Sbjct: 373 IYPDEFILENINSWTSRFLNHGLSGGSVHSDRTERLVKQEAVNAFEFPYNSTLERLSNKR 432
Query: 441 AIENYSGDTVRISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIENRLDK 500
A+E+YSGD VRISK+TYAC+NFGHQDF+ELAVEDFN LQCIHRKELKELE+WVIEN+LDK
Sbjct: 433 ALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVIENKLDK 492
Query: 501 LKFARQKLAYCYFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQL 560
LKFARQKLAYCYFSAAATLTSPE DARLSWAKNGVLTTVVDDFFD+GGSEEEL NLIQL
Sbjct: 493 LKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQL 552
Query: 561 VQKWDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLESMRK 620
V+KWDA+G A YCSKEVEIIFLALHSTICEIGE+AL WQGRSV RNVIDIWLALLESMRK
Sbjct: 553 VEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWLALLESMRK 612
Query: 621 EAEWLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQKLFKLMST 680
EAEWLKNKV PS+ EYMENGY SFALGPIVLPTLYFVGPKL E+IGNCEYQKLFKLMST
Sbjct: 613 EAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMST 672
Query: 681 CGRLLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELLRLVL 740
GRLLNDTRTFDRES+EGKLNA+SL +I+ GG LSKEEATEAMK DV+R RRELLRLVL
Sbjct: 673 SGRLLNDTRTFDRESSEGKLNALSLYLIS-AGGKLSKEEATEAMKGDVDRTRRELLRLVL 732
Query: 741 QEKNSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQELMNSAKALFEHPMVLDELLRK 798
QE NSTIPRACKDLFWKMSCVVHLFYRKDDGFTS ELMNSAKALFE PMVLDELL K
Sbjct: 733 QE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALFELPMVLDELLNK 785
BLAST of Sed0013143 vs. ExPASy TrEMBL
Match:
A0A5A7V5B3 (Ent-kaur-16-ene synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold357G00260 PE=4 SV=1)
HSP 1 Score: 1358.2 bits (3514), Expect = 0.0e+00
Identity = 672/785 (85.61%), Postives = 725/785 (92.36%), Query Frame = 0
Query: 13 FFTISVSSTLSISSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAW 72
F + + +S SSLFPGL+V+ KTK GALR FEDTKERI+KLF VELSVSAYDTAW
Sbjct: 79 FSLLPICFFVSTESSLFPGLDVDTKTKTGALR--FEDTKERIQKLFKNVELSVSAYDTAW 138
Query: 73 VAMVPSPNSLNKPLFPECIDWVLDNQHSDGSWGILHDHQLLMKATLLSTLACVLTLKRWN 132
VAMVPSPNSLN+PLFPECI+WVLD+Q+ DGSWGILHDHQL+MKATLLSTLACVLTLKRWN
Sbjct: 139 VAMVPSPNSLNRPLFPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWN 198
Query: 133 IGHNHITKALDFIKSNIASATDDKQQSPVGFNIIFPGMIEYAKDLNLNLPLAPMNLDALV 192
IGH+H++KAL FIKSNIASATD+ Q+SPVGF+IIFPGMIEYAKDLNLNLPLA MN+DALV
Sbjct: 199 IGHDHMSKALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALV 258
Query: 193 QKKQLELRSCRSNFEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVTFMH 252
QKK+LELRSC SN EGGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SPSTTAV FMH
Sbjct: 259 QKKELELRSCCSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMH 318
Query: 253 GNDDACFNYLSSLLQKFDGSVPSIYPLDIYARLHMVDNLQKLGIDRHFKEEIRSVLDETY 312
NDD CFNYL S+LQKF SVP+IYPLDIYARLHMVD+LQKLGIDRHFK+EIRSVLDETY
Sbjct: 319 RNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETY 378
Query: 313 RCWMQGEEDIFLDVSTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGYLKDFSASLE 372
RCWMQGEE+IFLD STCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGG+LKDF ASLE
Sbjct: 379 RCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLE 438
Query: 373 LFKASQIILHPDETVLENINSWTSRFLNHGLSSDSVCSDRTDRLVKQEAVNAFEFTYSAS 432
LFKASQII++PDE +LENINSWTSRFLNHGLS SV DRT+RLVKQEAVNAFEF Y+++
Sbjct: 439 LFKASQIIIYPDEFILENINSWTSRFLNHGLSGGSVHFDRTERLVKQEAVNAFEFPYNST 498
Query: 433 LERLLNKKAIENYSGDTVRISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERW 492
LERL NK+A+E+YSGD VRISK+TYAC+NFGHQDF+ELAVEDFN LQCIHRKELKELE+W
Sbjct: 499 LERLSNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKW 558
Query: 493 VIENRLDKLKFARQKLAYCYFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEE 552
VIEN+LDKLKFARQKLAYCYFSAAATLTSPE DARLSWAKNGVLTTVVDDFFD+GGSEE
Sbjct: 559 VIENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEE 618
Query: 553 ELTNLIQLVQKWDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWL 612
EL NLIQLV+KWDA+G A YCSKEVEIIFLALHSTICEIGE+AL WQGRSV RNVIDIWL
Sbjct: 619 ELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWL 678
Query: 613 ALLESMRKEAEWLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQ 672
ALLESMRKEAEWLKNKV PS+ EYMENGY SFALGPIVLPTLYFVGPKL E+IGNCEYQ
Sbjct: 679 ALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQ 738
Query: 673 KLFKLMSTCGRLLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMR 732
KLFKLMST GRLLNDTRTFDRES+EGKLNA+SL +I+ GG LSKEEATEAMK DV+R R
Sbjct: 739 KLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYLIS-AGGKLSKEEATEAMKGDVDRTR 798
Query: 733 RELLRLVLQEKNSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQELMNSAKALFEHPMVLD 792
RELLRLVLQE NSTIPRACKDLFWKMSCVVHLFYRKDDGFTS ELMNSAKALFE PMVLD
Sbjct: 799 RELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALFELPMVLD 858
Query: 793 ELLRK 798
ELL K
Sbjct: 859 ELLNK 859
BLAST of Sed0013143 vs. ExPASy TrEMBL
Match:
A0A1S4DWJ8 (ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489622 PE=4 SV=1)
HSP 1 Score: 1354.0 bits (3503), Expect = 0.0e+00
Identity = 672/777 (86.49%), Postives = 722/777 (92.92%), Query Frame = 0
Query: 21 TLSISSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAWVAMVPSPN 80
T S S+SLFPGL+V+ KTK GALR FEDTKERI+KLF VELSVSAYDTAWVAMVPSPN
Sbjct: 13 TPSSSASLFPGLDVDTKTKTGALR--FEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPN 72
Query: 81 SLNKPLFPECIDWVLDNQHSDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGHNHITK 140
SLNKPLFPECI+WVLD+Q+ DGSWGILHDHQL+MKATLLSTLACVLTLKRWNIGH+H++K
Sbjct: 73 SLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSK 132
Query: 141 ALDFIKSNIASATDDKQQSPVGFNIIFPGMIEYAKDLNLNLPLAPMNLDALVQKKQLELR 200
AL FIKSNIASATD+ Q+SPVGF+IIFPGMIEYAKDLNLNLPLA MN+DALVQKK+LEL
Sbjct: 133 ALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALVQKKELEL- 192
Query: 201 SCRSNFEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVTFMHGNDDACFN 260
RSN EGGKAYLAYVSEGIGKLQDW+MVMQYQRKNGSLF+SPSTTAV FMH NDD CFN
Sbjct: 193 --RSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFN 252
Query: 261 YLSSLLQKFDGSVPSIYPLDIYARLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWMQGEE 320
YL S+LQKF SVP+IYPLDIYARLHMVD+LQKLGIDRHFK+EIRSVLDETYRCWMQGEE
Sbjct: 253 YLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEE 312
Query: 321 DIFLDVSTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGYLKDFSASLELFKASQII 380
+IFLD STCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGG+LKDF ASLELFKASQII
Sbjct: 313 NIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGHLKDFGASLELFKASQII 372
Query: 381 LHPDETVLENINSWTSRFLNHGLSSDSVCSDRTDRLVKQEAVNAFEFTYSASLERLLNKK 440
++PDE +LENINSWTSRFLNHGLS SV SDRT+RLVKQEAVNAFEF Y+++LERL NK+
Sbjct: 373 IYPDEFILENINSWTSRFLNHGLSGGSVHSDRTERLVKQEAVNAFEFPYNSTLERLSNKR 432
Query: 441 AIENYSGDTVRISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIENRLDK 500
A+E+YSGD VRISK+TYAC+NFGHQDF+ELAVEDFN LQCIHRKELKELE+WVIEN+LDK
Sbjct: 433 ALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTLQCIHRKELKELEKWVIENKLDK 492
Query: 501 LKFARQKLAYCYFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQL 560
LKFARQKLAYCYFSAAATLTSPE DARLSWAKNGVLTTVVDDFFD+GGSEEEL NLIQL
Sbjct: 493 LKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQL 552
Query: 561 VQKWDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLESMRK 620
V+KWDA+G A YCSKEVEIIFLALHSTICEIGE+AL WQGRSV RNVIDIWLALLESMRK
Sbjct: 553 VEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKALPWQGRSVTRNVIDIWLALLESMRK 612
Query: 621 EAEWLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQKLFKLMST 680
EAEWLKNKV PS+ EYMENGY SFALGPIVLPTLYFVGPKL E+IGNCEYQKLFKLMST
Sbjct: 613 EAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMST 672
Query: 681 CGRLLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELLRLVL 740
GRLLNDTRTFDRES+EGKLNA+SL +I+ GG LSKEEATEAMK DV+R RRELLRLVL
Sbjct: 673 SGRLLNDTRTFDRESSEGKLNALSLYLIS-AGGKLSKEEATEAMKGDVDRTRRELLRLVL 732
Query: 741 QEKNSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQELMNSAKALFEHPMVLDELLRK 798
QE NSTIPRACKDLFWKMSCVVHLFYRKDDGFTS ELMNSAKALFE PMVLDELL K
Sbjct: 733 QE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALFELPMVLDELLNK 782
BLAST of Sed0013143 vs. ExPASy TrEMBL
Match:
A0A5D3BE57 (Ent-kaur-16-ene synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold986G00380 PE=4 SV=1)
HSP 1 Score: 1344.3 bits (3478), Expect = 0.0e+00
Identity = 672/810 (82.96%), Postives = 725/810 (89.51%), Query Frame = 0
Query: 13 FFTISVSSTLSISSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAW 72
F + + +S SSLFPGL+V+ KTK GALR FEDTKERI+KLF VELSVSAYDTAW
Sbjct: 79 FSLLPICFFVSTESSLFPGLDVDTKTKTGALR--FEDTKERIQKLFKNVELSVSAYDTAW 138
Query: 73 VAMVPSPNSLNKPLFPECIDWVLDNQHSDGSWGILHDHQLLMKATLLSTLACVLTLKRWN 132
VAMVPSPNSLN+PLFPECI+WVLD+Q+ DGSWGILHDHQL+MKATLLSTLACVLTLKRWN
Sbjct: 139 VAMVPSPNSLNRPLFPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWN 198
Query: 133 IGHNHITK-------------------------ALDFIKSNIASATDDKQQSPVGFNIIF 192
IGH+H++K AL FIKSNIASATD+ Q+SPVGF+IIF
Sbjct: 199 IGHDHMSKGMLTFLEFFFMKYSVMNSRHFLCFAALSFIKSNIASATDENQRSPVGFDIIF 258
Query: 193 PGMIEYAKDLNLNLPLAPMNLDALVQKKQLELRSCRSNFEGGKAYLAYVSEGIGKLQDWE 252
PGMIEYAKDLNLNLPLA MN+DALVQKK+LELRSC SN EGGKAYLAYVSEGIGKLQDW+
Sbjct: 259 PGMIEYAKDLNLNLPLASMNVDALVQKKELELRSCCSNSEGGKAYLAYVSEGIGKLQDWD 318
Query: 253 MVMQYQRKNGSLFNSPSTTAVTFMHGNDDACFNYLSSLLQKFDGSVPSIYPLDIYARLHM 312
MVMQYQRKNGSLF+SPSTTAV FMH NDD CFNYL S+LQKF SVP+IYPLDIYARLHM
Sbjct: 319 MVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLHM 378
Query: 313 VDNLQKLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDVSTCAMAFRMLRVEGYDVSSDQ 372
VD+LQKLGIDRHFK+EIRSVLDETYRCWMQGEE+IFLD STCAMAFRMLRVEGYDVSSDQ
Sbjct: 379 VDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQ 438
Query: 373 LTQFSEDLFPNCLGGYLKDFSASLELFKASQIILHPDETVLENINSWTSRFLNHGLSSDS 432
LTQFSEDLFPNCLGG+LKDF ASLELFKASQII++PDE +LENINSWTSRFLNHGLS S
Sbjct: 439 LTQFSEDLFPNCLGGHLKDFGASLELFKASQIIIYPDEFILENINSWTSRFLNHGLSGGS 498
Query: 433 VCSDRTDRLVKQEAVNAFEFTYSASLERLLNKKAIENYSGDTVRISKSTYACINFGHQDF 492
V DRT+RLVKQEAVNAFEF Y+++LERL NK+A+E+YSGD VRISK+TYAC+NFGHQDF
Sbjct: 499 VHFDRTERLVKQEAVNAFEFPYNSTLERLSNKRALESYSGDIVRISKTTYACLNFGHQDF 558
Query: 493 VELAVEDFNALQCIHRKELKELERWVIENRLDKLKFARQKLAYCYFSAAATLTSPEHRDA 552
+ELAVEDFN LQCIHRKELKELE+WVIEN+LDKLKFARQKLAYCYFSAAATLTSPE DA
Sbjct: 559 LELAVEDFNTLQCIHRKELKELEKWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCDA 618
Query: 553 RLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQLVQKWDANGGADYCSKEVEIIFLALHST 612
RLSWAKNGVLTTVVDDFFD+GGSEEEL NLIQLV+KWDA+G A YCSKEVEIIFLALHST
Sbjct: 619 RLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHST 678
Query: 613 ICEIGERALAWQGRSVMRNVIDIWLALLESMRKEAEWLKNKVAPSIKEYMENGYTSFALG 672
ICEIGE+AL WQGRSV RNVIDIWLALLESMRKEAEWLKNKV PS+ EYMENGY SFALG
Sbjct: 679 ICEIGEKALPWQGRSVTRNVIDIWLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALG 738
Query: 673 PIVLPTLYFVGPKLSPEVIGNCEYQKLFKLMSTCGRLLNDTRTFDRESNEGKLNAVSLRM 732
PIVLPTLYFVGPKL E+IGNCEYQKLFKLMST GRLLNDTRTFDRES+EGKLNA+SL +
Sbjct: 739 PIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYL 798
Query: 733 INGGGGNLSKEEATEAMKEDVERMRRELLRLVLQEKNSTIPRACKDLFWKMSCVVHLFYR 792
I+ GG LSKEEATEAMK DV+R RRELLRLVLQE NSTIPRACKDLFWKMSCVVHLFYR
Sbjct: 799 IS-AGGKLSKEEATEAMKGDVDRTRRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYR 858
Query: 793 KDDGFTSQELMNSAKALFEHPMVLDELLRK 798
KDDGFTS ELMNSAKALFE PMVLDELL K
Sbjct: 859 KDDGFTSHELMNSAKALFELPMVLDELLNK 884
BLAST of Sed0013143 vs. ExPASy TrEMBL
Match:
Q9FRX5 (Ent-kaurene synthase OS=Cucumis sativus OX=3659 GN=CsKS1 PE=2 SV=1)
HSP 1 Score: 1340.1 bits (3467), Expect = 0.0e+00
Identity = 664/777 (85.46%), Postives = 718/777 (92.41%), Query Frame = 0
Query: 21 TLSISSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAWVAMVPSPN 80
T S S+SLFPGL+V KTK GALR FE+TKERIKKLF VELS+SAYDTAWVAMVPSPN
Sbjct: 13 TASSSASLFPGLDVGTKTKTGALR--FEETKERIKKLFKNVELSISAYDTAWVAMVPSPN 72
Query: 81 SLNKPLFPECIDWVLDNQHSDGSWGILHDHQLLMKATLLSTLACVLTLKRWNIGHNHITK 140
SLNKPLFPECI+WVLD+Q+ DGSWGILHDHQL+MKATLLSTLACVLTLKRW+IG +H++K
Sbjct: 73 SLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKATLLSTLACVLTLKRWDIGDDHMSK 132
Query: 141 ALDFIKSNIASATDDKQQSPVGFNIIFPGMIEYAKDLNLNLPLAPMNLDALVQKKQLELR 200
AL FIKSNIASATD+ Q+SPVGF+IIFPGMIEYAKDLNLNLPLA MN+DALVQKK+LELR
Sbjct: 133 ALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLASMNVDALVQKKELELR 192
Query: 201 SCRSNFEGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVTFMHGNDDACFN 260
SC SN EGGKAYLAYVSEGIGKLQDWEMVM+YQRKNGSLF+SPSTTAV FMH NDD CFN
Sbjct: 193 SCCSNSEGGKAYLAYVSEGIGKLQDWEMVMRYQRKNGSLFSSPSTTAVAFMHRNDDGCFN 252
Query: 261 YLSSLLQKFDGSVPSIYPLDIYARLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWMQGEE 320
YL S+LQKF SVP+IYPLDIYARLHMVD+LQKLGID HFK+EIRSVLDETY CWMQGEE
Sbjct: 253 YLRSVLQKFHSSVPAIYPLDIYARLHMVDSLQKLGIDGHFKDEIRSVLDETYSCWMQGEE 312
Query: 321 DIFLDVSTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPNCLGGYLKDFSASLELFKASQII 380
+IFLD STCAMAFRMLRVEGYDVSSDQLTQFSE LF NCLGG+LKDFSASLELFKASQII
Sbjct: 313 NIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEGLFSNCLGGHLKDFSASLELFKASQII 372
Query: 381 LHPDETVLENINSWTSRFLNHGLSSDSVCSDRTDRLVKQEAVNAFEFTYSASLERLLNKK 440
++PDE +LENINSWTSRFLNHGLSS SV SDRT+RLVKQEAVNAFEF Y+++LERL NK+
Sbjct: 373 IYPDEFILENINSWTSRFLNHGLSSGSVHSDRTERLVKQEAVNAFEFPYNSTLERLSNKR 432
Query: 441 AIENYSGDTVRISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIENRLDK 500
A+E+YSGD VRISK+ YAC+NFGHQDF+ELAVEDFN LQ IHRKELKELE+WVIEN+LDK
Sbjct: 433 ALESYSGDIVRISKTAYACLNFGHQDFLELAVEDFNTLQGIHRKELKELEKWVIENKLDK 492
Query: 501 LKFARQKLAYCYFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQL 560
LKFARQKLAYCYFSAAATLTSPE DARLSWAKNGVLTTVVDDFFD+GGSEEEL NLIQL
Sbjct: 493 LKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQL 552
Query: 561 VQKWDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLESMRK 620
V+KWDA+G YCSKEVEIIFLALHSTICEIG++AL WQGRSVMRNVIDIWLALLESMRK
Sbjct: 553 VEKWDASGETGYCSKEVEIIFLALHSTICEIGKKALPWQGRSVMRNVIDIWLALLESMRK 612
Query: 621 EAEWLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQKLFKLMST 680
EAEWLKNKV PS+ EYMENGY SFALGPIVLPTLYFVGPKL E++GNCEY+KLFKLMST
Sbjct: 613 EAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYFVGPKLPEEIVGNCEYKKLFKLMST 672
Query: 681 CGRLLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELLRLVL 740
GRLLNDTRTFDRES+EGKLNA+SL MI+ GG L+KEEATEAMK DV+R RRELLRLVL
Sbjct: 673 SGRLLNDTRTFDRESSEGKLNALSLYMIS-AGGKLTKEEATEAMKGDVDRTRRELLRLVL 732
Query: 741 QEKNSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQELMNSAKALFEHPMVLDELLRK 798
QE NSTIPRACKDLFWKMSCVVHLFYRKDDGFTS ELMNSAKALFE PMVLDELL K
Sbjct: 733 QE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMNSAKALFEQPMVLDELLNK 785
BLAST of Sed0013143 vs. TAIR 10
Match:
AT1G79460.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )
HSP 1 Score: 820.1 bits (2117), Expect = 1.6e-237
Identity = 422/774 (54.52%), Postives = 552/774 (71.32%), Query Frame = 0
Query: 24 ISSSLFPGLEVEKKTKNGALRFEFEDTKERIKKLFNKVELSVSAYDTAWVAMVPSPNSLN 83
IS++L L+ E +T+ A FE TKE+I+K+ KVELSVSAYDT+WVAMVPSP+S N
Sbjct: 14 ISATLERRLDSEVQTR--ANNVSFEQTKEKIRKMLEKVELSVSAYDTSWVAMVPSPSSQN 73
Query: 84 KPLFPECIDWVLDNQHSDGSWGI-LHDHQLLMKATLLSTLACVLTLKRWNIGHNHITKAL 143
PLFP+C+ W+LDNQH DGSWG+ HDHQ L K L STLA +L LK+W IG I K L
Sbjct: 74 APLFPQCVKWLLDNQHEDGSWGLDNHDHQSLKKDVLSSTLASILALKKWGIGERQINKGL 133
Query: 144 DFIKSNIASATDDKQQSPVGFNIIFPGMIEYAKDLNLNLPLAPMNLDALVQKKQLELRSC 203
FI+ N A TD+ Q P GF+IIFPGMI+YA+DLNL +PL +D +++K+ L+L+
Sbjct: 134 QFIELNSALVTDETIQKPTGFDIIFPGMIKYARDLNLTIPLGSEVVDDMIRKRDLDLKCD 193
Query: 204 RSNF-EGGKAYLAYVSEGIGKLQDWEMVMQYQRKNGSLFNSPSTTAVTFMHGNDDACFNY 263
F +G +AYLAYV EG L+DW+++++YQRKNGSLF+SP+TTA F +D C Y
Sbjct: 194 SEKFSKGREAYLAYVLEGTRNLKDWDLIVKYQRKNGSLFDSPATTAAAFTQFGNDGCLRY 253
Query: 264 LSSLLQKFDGSVPSIYPLDIYARLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWMQGEED 323
L SLLQKF+ +VPS+YP D YARL ++ L+ LGIDR FK EI+S+LDETYR W++G+E+
Sbjct: 254 LCSLLQKFEAAVPSVYPFDQYARLSIIVTLESLGIDRDFKTEIKSILDETYRYWLRGDEE 313
Query: 324 IFLDVSTCAMAFRMLRVEGYDVSSDQLTQFSEDL-FPNCLGGYLKDFSASLELFKASQII 383
I LD++TCA+AFR+L GYDVS D L F+E+ F + L GY+K+ + LELFKA+Q
Sbjct: 314 ICLDLATCALAFRLLLAHGYDVSYDPLKPFAEESGFSDTLEGYVKNTFSVLELFKAAQ-- 373
Query: 384 LHPDETVLENINSWTSRFLNHGLSSDSVCSDRTDRLVKQEAVNAFEFTYSASLERLLNKK 443
+P E+ L+ WT ++L LSS S R D+ +K+E +A F ASLER +++
Sbjct: 374 SYPHESALKKQCCWTKQYLEMELSSWVKTSVR-DKYLKKEVEDALAFPSYASLERSDHRR 433
Query: 444 AIENYSG-DTVRISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIENRLD 503
I N S + R++K++Y N D ++LAV+DFN Q IHR+E++ L+RW++ENRL
Sbjct: 434 KILNGSAVENTRVTKTSYRLHNICTSDILKLAVDDFNFCQSIHREEMERLDRWIVENRLQ 493
Query: 504 KLKFARQKLAYCYFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQ 563
+LKFARQKLAYCYFS AATL SPE DAR+SWAK GVLTTVVDDFFD+GGS+EEL NLI
Sbjct: 494 ELKFARQKLAYCYFSGAATLFSPELSDARISWAKGGVLTTVVDDFFDVGGSKEELENLIH 553
Query: 564 LVQKWDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLESMR 623
LV+KWD NG +Y S+ VEIIF L TI E G++A +QGR+V +++ IWL LL+SM
Sbjct: 554 LVEKWDLNGVPEYSSEHVEIIFSVLRDTILETGDKAFTYQGRNVTHHIVKIWLDLLKSML 613
Query: 624 KEAEWLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQKLFKLMS 683
+EAEW +K PS+++YMEN Y SFALGPIVLP Y +GP L + + + +Y +L+KL+S
Sbjct: 614 REAEWSSDKSTPSLEDYMENAYISFALGPIVLPATYLIGPPLPEKTVDSHQYNQLYKLVS 673
Query: 684 TCGRLLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELLRLV 743
T GRLLND + F RES EGKLNAVSL M N SKE E+MK ER R EL +LV
Sbjct: 674 TMGRLLNDIQGFKRESAEGKLNAVSLHM-KHERDNRSKEVIIESMKGLAERKREELHKLV 733
Query: 744 LQEKNSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQELMNSAKALFEHPMVLDE 794
L+EK S +PR CK+ F KMS V++LFYRKDDGFTS +LM+ K++ P+ L E
Sbjct: 734 LEEKGSVVPRECKEAFLKMSKVLNLFYRKDDGFTSNDLMSLVKSVIYEPVSLQE 781
BLAST of Sed0013143 vs. TAIR 10
Match:
AT4G02780.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )
HSP 1 Score: 280.4 bits (716), Expect = 4.5e-75
Identity = 165/514 (32.10%), Postives = 271/514 (52.72%), Query Frame = 0
Query: 47 FEDTKERIKKLFNKV---ELSVSAYDTAWVAMVPSPNSLNKPLFPECIDWVLDNQHSDGS 106
F++ + +K + + E+++SAYDTAWVA++ + + P FP + W+ +NQ SDGS
Sbjct: 94 FKEAVKSVKTILRNLTDGEITISAYDTAWVALIDAGD--KTPAFPSAVKWIAENQLSDGS 153
Query: 107 WGILHDHQLLMKATLLSTLACVLTLKRWNIGHNHITKALDFIKSNIASATDDK-QQSPVG 166
WG + L++TLACV+ L+ WN+ + K + F + NI D+ + P+G
Sbjct: 154 WG--DAYLFSYHDRLINTLACVVALRSWNLFPHQCNKGITFFRENIGKLEDENDEHMPIG 213
Query: 167 FNIIFPGMIEYAKDLNLNLPLAPMNLDALVQKKQLEL-RSCRSNFEGGKAYLAYVSEGIG 226
F + FP ++E A+ +N+++P L + KK+L+L R + L + EG+
Sbjct: 214 FEVAFPSLLEIARGINIDVPYDSPVLKDIYAKKELKLTRIPKEIMHKIPTTLLHSLEGMR 273
Query: 227 KLQDWEMVMQYQRKNGSLFNSPSTTAVTFMHGNDDACFNYLSSLLQKFDGSVPSIYPLDI 286
L DWE +++ Q ++GS SPS+TA FM D C YL + +++F+G VP+++P+D+
Sbjct: 274 DL-DWEKLLKLQSQDGSFLFSPSSTAFAFMQTRDSNCLEYLRNAVKRFNGGVPNVFPVDL 333
Query: 287 YARLHMVDNLQKLGIDRHFKEEIRSVLDETYR-------CWMQGEEDIFLDVSTCAMAFR 346
+ + +VD LQ+LGI R+F+EEI+ LD +R CW + D+ AMAFR
Sbjct: 334 FEHIWIVDRLQRLGISRYFEEEIKECLDYVHRYWTDNGICWARCSH--VQDIDDTAMAFR 393
Query: 347 MLRVEGYDVSSDQLTQFSEDLFPNCLGGYLKD-FSASLELFKASQIILHPDETVLENINS 406
+LR GY VS+D F ++ C G + L++ASQ+ P E +L+N
Sbjct: 394 LLRQHGYQVSADVFKNFEKEGEFFCFVGQSNQAVTGMFNLYRASQLAF-PREEILKNAKE 453
Query: 407 WTSRFLNHGLSSDSVCSD-RTDRLVKQEAVNAFEFTYSASLERLLNKKAIENYSGDT-VR 466
++ +L + + + + E A E + ASL R+ + I+ Y G+ V
Sbjct: 454 FSYNYLLEKREREELIDKWIIMKDLPGEIGFALEIPWYASLPRVETRFYIDQYGGENDVW 513
Query: 467 ISKSTYACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIENRLDKLKFARQKLAYC 526
I K+ Y + ++ELA +D+N Q H+ E ++W ENRL + R +L C
Sbjct: 514 IGKTLYRMPYVNNNGYLELAKQDYNNCQAQHQLEWDIFQKWYEENRLSEWGVRRSELLEC 573
Query: 527 YFSAAATLTSPEHRDARLSWAKNGVLTTVVDDFF 546
Y+ AAAT+ E R+ WAK+ VL + F
Sbjct: 574 YYLAAATIFESERSHERMVWAKSSVLVKAISSSF 599
BLAST of Sed0013143 vs. TAIR 10
Match:
AT1G61120.1 (terpene synthase 04 )
HSP 1 Score: 280.0 bits (715), Expect = 5.8e-75
Identity = 220/775 (28.39%), Postives = 372/775 (48.00%), Query Frame = 0
Query: 63 LSVSAYDTAWVAMVPSPNSLN----KPLFPECIDWVLDNQHS-DGSW-----------GI 122
+S SAYDTAW++M+ +++ KP+F C+DW++ NQ++ +G W G
Sbjct: 36 VSPSAYDTAWLSMIEEDINVDDNELKPMFQGCLDWIMCNQNAREGFWMNSTSYTTVADGR 95
Query: 123 LHDHQLLMKATLLSTLACVLTLKRWNIGHNHITKALDFIKSN----IASATDDKQQSPVG 182
D + M L STLACV+ L++WNIG H+ K +I+ N I +++ P
Sbjct: 96 DEDGEKDM-CILTSTLACVVALQKWNIGCFHLHKGTRYIERNTEMIIGKYINEEGSYPRW 155
Query: 183 FNIIFPGMIEYAKDLNLNLPLAPMNLDAL----VQKKQLELRSCRSNFEGGKAYLAYVSE 242
F I F G++E A+ L L+ + ++ + Q++++ R + K LAY+
Sbjct: 156 FAIKFTGILELAQKLGLHFVFSSRCIEMIKGMFYQRQEIIQREKLVHDCNYKPLLAYLEV 215
Query: 243 GIGKL----QDWEMVMQYQRKNGSLFNSPSTTAVTFMHGNDDACFNYLSSLLQKFDGSVP 302
KL Q+ +V +GSLF SPS TA FM + C YL +L+QK VP
Sbjct: 216 LPSKLYVTNQEDIIVKSLDSMDGSLFQSPSATASAFMLTRNTKCLAYLQNLVQKCPNGVP 275
Query: 303 SIYPL-DIYARLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWMQGEED------IFLDVS 362
YPL + +L MV+ ++ G+ F EI VL++ Y + + + + + +
Sbjct: 276 QKYPLNEDLIKLSMVNLIESTGLGEFFGIEIEHVLEQVYSRYEEKDFERMPMSYLADQLH 335
Query: 363 TCAMAFRMLRVEGYDVSSDQLTQFSEDL-FPNCLGGYLKDF-SASLELFKASQIILHPDE 422
++AFRMLR+ G DVS F D N L + F L +++A+ ++ P E
Sbjct: 336 KDSLAFRMLRMHGRDVSPRSFCWFLNDQETRNHLERNIDSFLLVILSVYRATD-LMFPGE 395
Query: 423 TVLENINSWTSRFLNHGLSSDSVCSDRTDRLVKQEAVNAFEFTYSASLERLLNKKAIENY 482
L+ +T L ++ + ++ ++ + A L+ L ++ IE+
Sbjct: 396 HDLQEAREYTRNLL-----------EKRRSIKEKMIMHELSTPWIARLKHLDHRMWIEDK 455
Query: 483 SGDTVRISKSTYACINFGHQD-FVELAVEDFNALQCIHRKELKELERWVIENRLDKLKFA 542
+ + + + K+++ ++ + D LA +F Q + +EL+EL WV + L + F
Sbjct: 456 NSNVLSMEKASFLRLHSSYSDKLTHLAARNFEFQQAKYCRELEELTMWVKKWGLSDIGFG 515
Query: 543 RQKLAYCYFSAAATLTSPEHRDA----RLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQL 602
R+K YCYF AT+TS + A +L+ AK +L T+ DDFFD GS +L L +
Sbjct: 516 REKTTYCYF---ATVTSLPYEYAIKFGKLA-AKTAILITIADDFFDEKGSFNDLEGLTKA 575
Query: 603 VQKWDANGGADYCSKEVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLESMRK 662
V +W+ Y + IIF AL + E ++ ++ +IW ES +
Sbjct: 576 VLRWEGEELKSYGN----IIFRALDDIVRETANTCRTHHKTDIIVHLRNIWGETFESWLR 635
Query: 663 EAEWLKNKVAPSIKEYMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQKLFKLMST 722
EAEW K S+ EY+ NG S A I L + P + Y + L+
Sbjct: 636 EAEWSKKGHTSSMDEYIRNGMISIAAHTIALSISCLMEPCFPHNKLKPGNYDSITTLLMI 695
Query: 723 CGRLLNDTRTFDRESNEGKLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELLRLVL 782
RLLND +++ +E +GK+N+V L M N G L E++ +++ ++ R+E L VL
Sbjct: 696 IPRLLNDLQSYQKEQEQGKMNSVLLHMKNHPG--LEIEDSIAHIEKIIDSKRKEFLEHVL 755
Query: 783 QEKNSTIPRACKDLFWKMSCVVHLFYRKDDGFTSQ-ELMNSAKALFEHPMVLDEL 795
+ S +P+ CK++ V +F+ K + + S E+++ K P+ + EL
Sbjct: 756 VDGLSDLPKPCKEIHMSCCKVFEMFFNKKNRYDSNTEMLHDIKKALYDPINVYEL 787
BLAST of Sed0013143 vs. TAIR 10
Match:
AT4G16740.1 (terpene synthase 03 )
HSP 1 Score: 156.4 bits (394), Expect = 9.7e-38
Identity = 134/521 (25.72%), Postives = 244/521 (46.83%), Query Frame = 0
Query: 284 RLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWMQG-----EEDIFLDVSTCAMAFRMLRV 343
+L ++D LQ+LG+ HF++EI+ L + ++ + + + D+ A+ FR+LR
Sbjct: 77 QLELIDTLQRLGVSYHFEQEIKKTLTNVHVKNVRAHKNRIDRNRWGDLYATALEFRLLRQ 136
Query: 344 EGYDVSSDQLTQFSEDLFPNCLGGYL--KDFSASLELFKASQIILHPDETVLENINSWTS 403
G+ + ++D+F +G L KD L L++AS + D + E+I T
Sbjct: 137 HGFSI--------AQDVFDGNIGVDLDDKDIKGILSLYEASYLSTRIDTKLKESIYYTTK 196
Query: 404 RFLNHGLSSDSVCSDRTDRLVKQEAVNAFEFTYSASLERLLNKKAIENYSGDTVRISKST 463
R + + T +++ ++A E Y + RL + IE Y G+ ++
Sbjct: 197 RLRKFVEVNKNETKSYT---LRRMVIHALEMPYHRRVGRLEARWYIEVY-GERHDMNPI- 256
Query: 464 YACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIENRLDK-LKFARQKLAYCYFSA 523
+ELA DFN +Q IH+ ELK L W + L K L F R ++ YFS+
Sbjct: 257 ----------LLELAKLDFNFVQAIHQDELKSLSSWWSKTGLTKHLDFVRDRITEGYFSS 316
Query: 524 AATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQLVQKWDANGGADYCSK 583
+ PE R K +L T +DD +DI G+ EEL +V+KWD N +
Sbjct: 317 VGVMYEPEFAYHRQMLTKVFMLITTIDDIYDIYGTLEELQLFTTIVEKWDVN-RLEELPN 376
Query: 584 EVEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLESMRKEAEWLKNKVAPSIKE 643
+++ FL L + I +IG L +G +V+ + + W + + KEA+W K+ P+ +E
Sbjct: 377 YMKLCFLCLVNEINQIGYFVLRDKGFNVIPYLKESWADMCTTFLKEAKWYKSGYKPNFEE 436
Query: 644 YMENGYTSFALGPIVLPTLYFVGPKLSPEVIGNCEYQKLFKLMSTCGRLLNDTRTFDRES 703
YM+NG+ S ++ P +L L+ + + +++G+ + + + +T RL ND T E
Sbjct: 437 YMQNGWISSSV-PTILLHLFCLLSDQTLDILGSYNH-SVVRSSATILRLANDLATSSEEL 496
Query: 704 NEG-KLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELLRLVLQEKNSTIPRACKDL 763
G + +V M G + E + A + + + + L +++K+ + + +
Sbjct: 497 ARGDTMKSVQCHMHETG----ASEAESRAYIQGIIGVAWDDLN--MEKKSCRLHQGFLEA 556
Query: 764 FWKMSCVVHLFYRKDDGF---TSQELMNSAKALFEHPMVLD 793
+ V Y+ DG + +N ++L HP+ L+
Sbjct: 557 AANLGRVAQCVYQYGDGHGCPDKAKTVNHVRSLLVHPLPLN 565
BLAST of Sed0013143 vs. TAIR 10
Match:
AT4G16730.1 (terpene synthase 02 )
HSP 1 Score: 152.9 bits (385), Expect = 1.1e-36
Identity = 138/502 (27.49%), Postives = 221/502 (44.02%), Query Frame = 0
Query: 284 RLHMVDNLQKLGIDRHFKEEIRSVLDETYRCWMQGE-EDIFLDVSTCAMAFRMLRVEGYD 343
+L M+D+LQ+LGI H+K EI +L + + GE E D+ ++ F +LR G+D
Sbjct: 44 QLEMIDSLQRLGISYHYKHEIHDILRKIHD--QHGEIERETQDLHATSLEFILLRQHGFD 103
Query: 344 VSSDQLTQFSEDLFPNCLGGYLK----DFSASLELFKASQIILHPDETVLENINSWTSRF 403
VS D D+F + G + K D L L++AS + E L+ + ++
Sbjct: 104 VSQDAF-----DVFISETGEFRKTLHSDIKGLLSLYEASYFSM-DSEFKLKETRIYANKR 163
Query: 404 LNHGLSSDSVCSDRTDRLVKQEAV-NAFEFTYSASLERLLNKKAIENYSGDTVRISKSTY 463
L+ ++ S R D E V A E Y S+ RL R + Y
Sbjct: 164 LSEFVAESSKTICREDETYILEMVKRALETPYHWSIRRL------------EARWYINVY 223
Query: 464 ACINFGHQDFVELAVEDFNALQCIHRKELKELERWVIENRLDK-LKFARQKLAYCYFSAA 523
+ + +E A DFN LQ H++ELK + W L K L F R ++ YF
Sbjct: 224 EKKHEMNPLLLEFAAIDFNMLQANHQEELKLISSWWNSTGLMKQLDFVRDRITESYFWTI 283
Query: 524 ATLTSPEHRDARLSWAKNGVLTTVVDDFFDIGGSEEELTNLIQLVQKWDANGGADYCSKE 583
PE + R K +L ++DD +DI G+ EEL +V+KWD N +
Sbjct: 284 GIFYEPEFKYCRKILTKIFMLIVIMDDIYDIYGTLEELELFTNVVEKWDVN-HVERLPNY 343
Query: 584 VEIIFLALHSTICEIGERALAWQGRSVMRNVIDIWLALLESMRKEAEWLKNKVAPSIKEY 643
+ + FL L++ I +IG L +G +V+ + +W L ++ E++W K PS +EY
Sbjct: 344 MRMCFLFLYNEINQIGYDVLRDKGLNVIPYLKQVWTDLFKTFLTESKWYKTGHKPSFEEY 403
Query: 644 MENGYTSFALGPIVLPTLYFVGPKLSPEVI-GNCEYQKLFKLMSTCGRLLNDTRTFDRES 703
M+NG S ++ I+L + +S + + + + + + +T RL ND T E
Sbjct: 404 MQNGVISSSVPTILLHLFSVLSDHISDQTLTDDSKNHSVVRSCATILRLANDLATSTEEM 463
Query: 704 NEG-KLNAVSLRMINGGGGNLSKEEATEAMKEDVERMRRELLRLVLQEKNSTIPRACKDL 763
G +V M S+EEA M+ + + + S++PR
Sbjct: 464 ARGDSPKSVQCYMYE---TRASEEEARRHMQSMISDSWDIINSDLKTAHTSSLPRGFLAA 521
Query: 764 FWKMSCVVHLFYRKDDGFTSQE 777
++ VV YR DG S E
Sbjct: 524 AANLNRVVQCIYRHGDGHGSPE 521
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_008447073.1 | 0.0e+00 | 86.74 | PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X1 [Cucumis melo] | [more] |
KAA0062808.1 | 0.0e+00 | 85.61 | ent-kaur-16-ene synthase [Cucumis melo var. makuwa] | [more] |
XP_016900364.1 | 0.0e+00 | 86.49 | PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X2 [Cucumis melo] | [more] |
XP_038888074.1 | 0.0e+00 | 85.97 | ent-kaur-16-ene synthase, chloroplastic isoform X3 [Benincasa hispida] | [more] |
XP_038888071.1 | 0.0e+00 | 85.97 | ent-kaur-16-ene synthase, chloroplastic isoform X1 [Benincasa hispida] >XP_03888... | [more] |
Match Name | E-value | Identity | Description | |
Q39548 | 0.0e+00 | 78.87 | Ent-kaur-16-ene synthase, chloroplastic OS=Cucurbita maxima OX=3661 PE=1 SV=1 | [more] |
A0A2K9RFY0 | 3.6e-247 | 55.47 | Ent-kaurene synthase TSP4, chloroplastic OS=Vitex agnus-castus OX=54477 GN=TPS4 ... | [more] |
A0A1Z3GC64 | 1.7e-244 | 54.73 | Ent-kaurene synthase 5, chloroplastic OS=Isodon rubescens OX=587669 GN=KSL5 PE=1... | [more] |
A0A0U4CDK4 | 1.3e-241 | 53.38 | Ent-kaur-16-ene synthase, chloroplastic OS=Salvia miltiorrhiza OX=226208 GN=KS P... | [more] |
A0A3G1QTS7 | 1.1e-240 | 54.22 | Ent-kaurene synthase 1, chloroplastic OS=Isodon eriocalyx OX=662907 GN=KS1 PE=1 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3BG06 | 0.0e+00 | 86.74 | ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LO... | [more] |
A0A5A7V5B3 | 0.0e+00 | 85.61 | Ent-kaur-16-ene synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... | [more] |
A0A1S4DWJ8 | 0.0e+00 | 86.49 | ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LO... | [more] |
A0A5D3BE57 | 0.0e+00 | 82.96 | Ent-kaur-16-ene synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... | [more] |
Q9FRX5 | 0.0e+00 | 85.46 | Ent-kaurene synthase OS=Cucumis sativus OX=3659 GN=CsKS1 PE=2 SV=1 | [more] |