Sed0012789 (gene) Chayote v1

Overview
NameSed0012789
Typegene
OrganismSechium edule (Chayote v1)
DescriptiontolB protein-related
LocationLG07: 4684416 .. 4686719 (-)
RNA-Seq ExpressionSed0012789
SyntenySed0012789
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACGAACAAACCTCATCTCTGACGTAACACTCCACTTCCATTCCCTATAAATATGCCCTTCCCAAAAGCTTCCTCAATCACTCTTCACTTTTGCACAATGGACAAGAACACCCCCACCGGCGCCGTCGTCTTCGCCACCGTCGGCCGCCCCCAGTACGGCTTCGACACCTTCTCCGTTTCCCTCCAATCCCCCACCGCCGAGCAACGCCTCACCGACGGAATCTCCGTCAATTTCAACGCCCAGTTCATCGACAACCAAAACTCCATCGTCTTCATCTCCGAACGATCCGGTTCCCCTAAAATCTACCGCTCCGATTCCCCCAATTCCGCCCCCGAATTGCTCCCCTCCGCCACCGGCAGCTTCTTCCACGACCGACCTATCATCAGAAATGGCCGCCTCTTCTTCACCTCCGCTCACGAAAACCCCCACAAGTCATTTGCGAGCTGGTCTGCTCTGTATTCCTCTGTTTTGGACGGATCCGACCCGGTAACCCGCCTGACTCCTCCCGGGTTTGTGGACTTCAGTCCGGCGGTTTCTGAGTCTGGGAAATTCGTGGCGGTTGCTTCTTATGGCTCTCGTCCTTGGGCCGGAGATTTTAACGAGCTCGCAACGGAAATTGTTGTGTTCGAAGCGGACAACCCGACCCGTCGAGTCGTCGTTTCGGGTCACGGCGGGTGGCCGTCGTGGTGCGGTGACTCTGCCGTGTATTTTCACCGGACTGCCGACGATGGGTGGTGGAGCATTTTCAGAGTGGAGATTCCTGAGAATCTCGAGTCCTCGGTTTCTTCTGTTCCGGTTCGGGTCACTCCGGCGGGTCTCCATTGCTTCACTCCGGCTGCCATGAACGACGGCAAACGAGTCGTCGTCGCCACTCGACGACCCGACGATAAATTCCGACACATCGAGATTTTCGATTCTGGGTCGGATGAATTCGACCCAATTACCAAGAAATTGAATCCGGGTTTCCACCATTACAACCCGTTTGTATCGCCCGATTCAAAATCCGTTGGGTACCACCGATTCCGAGGCGAGTCAACTCAAGGCGAGTTAACAGTTCCACATCTCGACTCGGTAATTTCCCCGATTAATAATCTTCGTATGATCCGAATAAACGGTTTTTTTCCAACGCCGTCACCTGACAACGATTTCATCGCATATAATCCCGATTTTGTGGGGTTAAAAATCGTCAAATCAGACAGCTCGAAGTCTTGGACCGTACTGAAAGATCGGACCGCTTTTTACAACTCGTGGAGCCCAACCGAAAAAAACGTGATTTTCACTTCTCTGGGACCCATTTTTGGACCGGCGAGAGCGACGGTTCAGATCGCTCGGATCACAATCAACTACGAAGATCTCAACGGTGGCGATGAAGATGAAATTGACCATGAAGTGAAGATTCTCACAAAAGAGGACACCGGGAATAACGCCTTTCCGGCGTGTTCGCCGGACGGGAGGTTTCTGGTTTTCCGATCCGGCCGGTCCGGGCATAAGAATCTTTACATCGTCGACGCCGTGAACGGAGAGCACGACGGCGACGTACGGCGGTTGACCGACGGTCCGTGGATCGACACAATGCCGAGTTGGTCGCCGACCGGAGAATTCATAGCGTTTTCTTCAAACATGCATAACCCGAAAAATACCGAAGCATTTAGTATCTACGTCATTAAACCGGACGGTTCGGGTTTAAGGAGGGTGCACGTGGCTGGACCGGAAGGGTCTGATGAAGTGGACCGTGAGAGGATTAACCACGTGTGCTTCAGTAAAGATGGGGAGTGGCTTTTATTCACGTCAAATTTGGCTGGGGTGACGTCAGAGCCAGTTTCTTTGCCCAATCAGTTTCAGCCATATGGCGATTTGTATGTCGTGAGGTTGGATGGGACTGGGCTGAGGAGGCTGACGTGGAATGGATATGAGAACGGAACTCCCACGTGGCACTACGGGAGTGAGCTGGCGCTTTCTGGGCTGAGTTTGAAAGATGACGTGGTTGGAGAGAAGTTGGAGGGGAAGTTTGAAGAACCCCTTTGGATAACGTTCAAATGATGACGTGTTATATGCCCACGTGGAGGTTTTCATTTTCAGTTGTTGGTTGAATAATAGAATAAAATAAATCATATCTTATCATGGAATTTGATCCTGTTACCTAAAAAGTTTGCAATTTATAAATCTTTATTCGAAAGAGATTGTAATCGTAATGATTTAGTTGGAATTATCCAACCTACTGTGCTTTTATATGTATCAAAATAAAATTAATTAGAAGAGTGGTTTGAATTGGAATTATTTATAATTTCATTTTAATGGGTGC

mRNA sequence

CAACGAACAAACCTCATCTCTGACGTAACACTCCACTTCCATTCCCTATAAATATGCCCTTCCCAAAAGCTTCCTCAATCACTCTTCACTTTTGCACAATGGACAAGAACACCCCCACCGGCGCCGTCGTCTTCGCCACCGTCGGCCGCCCCCAGTACGGCTTCGACACCTTCTCCGTTTCCCTCCAATCCCCCACCGCCGAGCAACGCCTCACCGACGGAATCTCCGTCAATTTCAACGCCCAGTTCATCGACAACCAAAACTCCATCGTCTTCATCTCCGAACGATCCGGTTCCCCTAAAATCTACCGCTCCGATTCCCCCAATTCCGCCCCCGAATTGCTCCCCTCCGCCACCGGCAGCTTCTTCCACGACCGACCTATCATCAGAAATGGCCGCCTCTTCTTCACCTCCGCTCACGAAAACCCCCACAAGTCATTTGCGAGCTGGTCTGCTCTGTATTCCTCTGTTTTGGACGGATCCGACCCGGTAACCCGCCTGACTCCTCCCGGGTTTGTGGACTTCAGTCCGGCGGTTTCTGAGTCTGGGAAATTCGTGGCGGTTGCTTCTTATGGCTCTCGTCCTTGGGCCGGAGATTTTAACGAGCTCGCAACGGAAATTGTTGTGTTCGAAGCGGACAACCCGACCCGTCGAGTCGTCGTTTCGGGTCACGGCGGGTGGCCGTCGTGGTGCGGTGACTCTGCCGTGTATTTTCACCGGACTGCCGACGATGGGTGGTGGAGCATTTTCAGAGTGGAGATTCCTGAGAATCTCGAGTCCTCGGTTTCTTCTGTTCCGGTTCGGGTCACTCCGGCGGGTCTCCATTGCTTCACTCCGGCTGCCATGAACGACGGCAAACGAGTCGTCGTCGCCACTCGACGACCCGACGATAAATTCCGACACATCGAGATTTTCGATTCTGGGTCGGATGAATTCGACCCAATTACCAAGAAATTGAATCCGGGTTTCCACCATTACAACCCGTTTGTATCGCCCGATTCAAAATCCGTTGGGTACCACCGATTCCGAGGCGAGTCAACTCAAGGCGAGTTAACAGTTCCACATCTCGACTCGGTAATTTCCCCGATTAATAATCTTCGTATGATCCGAATAAACGGTTTTTTTCCAACGCCGTCACCTGACAACGATTTCATCGCATATAATCCCGATTTTGTGGGGTTAAAAATCGTCAAATCAGACAGCTCGAAGTCTTGGACCGTACTGAAAGATCGGACCGCTTTTTACAACTCGTGGAGCCCAACCGAAAAAAACGTGATTTTCACTTCTCTGGGACCCATTTTTGGACCGGCGAGAGCGACGGTTCAGATCGCTCGGATCACAATCAACTACGAAGATCTCAACGGTGGCGATGAAGATGAAATTGACCATGAAGTGAAGATTCTCACAAAAGAGGACACCGGGAATAACGCCTTTCCGGCGTGTTCGCCGGACGGGAGGTTTCTGGTTTTCCGATCCGGCCGGTCCGGGCATAAGAATCTTTACATCGTCGACGCCGTGAACGGAGAGCACGACGGCGACGTACGGCGGTTGACCGACGGTCCGTGGATCGACACAATGCCGAGTTGGTCGCCGACCGGAGAATTCATAGCGTTTTCTTCAAACATGCATAACCCGAAAAATACCGAAGCATTTAGTATCTACGTCATTAAACCGGACGGTTCGGGTTTAAGGAGGGTGCACGTGGCTGGACCGGAAGGGTCTGATGAAGTGGACCGTGAGAGGATTAACCACGTGTGCTTCAGTAAAGATGGGGAGTGGCTTTTATTCACGTCAAATTTGGCTGGGGTGACGTCAGAGCCAGTTTCTTTGCCCAATCAGTTTCAGCCATATGGCGATTTGTATGTCGTGAGGTTGGATGGGACTGGGCTGAGGAGGCTGACGTGGAATGGATATGAGAACGGAACTCCCACGTGGCACTACGGGAGTGAGCTGGCGCTTTCTGGGCTGAGTTTGAAAGATGACGTGGTTGGAGAGAAGTTGGAGGGGAAGTTTGAAGAACCCCTTTGGATAACGTTCAAATGATGACGTGTTATATGCCCACGTGGAGGTTTTCATTTTCAGTTGTTGGTTGAATAATAGAATAAAATAAATCATATCTTATCATGGAATTTGATCCTGTTACCTAAAAAGTTTGCAATTTATAAATCTTTATTCGAAAGAGATTGTAATCGTAATGATTTAGTTGGAATTATCCAACCTACTGTGCTTTTATATGTATCAAAATAAAATTAATTAGAAGAGTGGTTTGAATTGGAATTATTTATAATTTCATTTTAATGGGTGC

Coding sequence (CDS)

ATGCCCTTCCCAAAAGCTTCCTCAATCACTCTTCACTTTTGCACAATGGACAAGAACACCCCCACCGGCGCCGTCGTCTTCGCCACCGTCGGCCGCCCCCAGTACGGCTTCGACACCTTCTCCGTTTCCCTCCAATCCCCCACCGCCGAGCAACGCCTCACCGACGGAATCTCCGTCAATTTCAACGCCCAGTTCATCGACAACCAAAACTCCATCGTCTTCATCTCCGAACGATCCGGTTCCCCTAAAATCTACCGCTCCGATTCCCCCAATTCCGCCCCCGAATTGCTCCCCTCCGCCACCGGCAGCTTCTTCCACGACCGACCTATCATCAGAAATGGCCGCCTCTTCTTCACCTCCGCTCACGAAAACCCCCACAAGTCATTTGCGAGCTGGTCTGCTCTGTATTCCTCTGTTTTGGACGGATCCGACCCGGTAACCCGCCTGACTCCTCCCGGGTTTGTGGACTTCAGTCCGGCGGTTTCTGAGTCTGGGAAATTCGTGGCGGTTGCTTCTTATGGCTCTCGTCCTTGGGCCGGAGATTTTAACGAGCTCGCAACGGAAATTGTTGTGTTCGAAGCGGACAACCCGACCCGTCGAGTCGTCGTTTCGGGTCACGGCGGGTGGCCGTCGTGGTGCGGTGACTCTGCCGTGTATTTTCACCGGACTGCCGACGATGGGTGGTGGAGCATTTTCAGAGTGGAGATTCCTGAGAATCTCGAGTCCTCGGTTTCTTCTGTTCCGGTTCGGGTCACTCCGGCGGGTCTCCATTGCTTCACTCCGGCTGCCATGAACGACGGCAAACGAGTCGTCGTCGCCACTCGACGACCCGACGATAAATTCCGACACATCGAGATTTTCGATTCTGGGTCGGATGAATTCGACCCAATTACCAAGAAATTGAATCCGGGTTTCCACCATTACAACCCGTTTGTATCGCCCGATTCAAAATCCGTTGGGTACCACCGATTCCGAGGCGAGTCAACTCAAGGCGAGTTAACAGTTCCACATCTCGACTCGGTAATTTCCCCGATTAATAATCTTCGTATGATCCGAATAAACGGTTTTTTTCCAACGCCGTCACCTGACAACGATTTCATCGCATATAATCCCGATTTTGTGGGGTTAAAAATCGTCAAATCAGACAGCTCGAAGTCTTGGACCGTACTGAAAGATCGGACCGCTTTTTACAACTCGTGGAGCCCAACCGAAAAAAACGTGATTTTCACTTCTCTGGGACCCATTTTTGGACCGGCGAGAGCGACGGTTCAGATCGCTCGGATCACAATCAACTACGAAGATCTCAACGGTGGCGATGAAGATGAAATTGACCATGAAGTGAAGATTCTCACAAAAGAGGACACCGGGAATAACGCCTTTCCGGCGTGTTCGCCGGACGGGAGGTTTCTGGTTTTCCGATCCGGCCGGTCCGGGCATAAGAATCTTTACATCGTCGACGCCGTGAACGGAGAGCACGACGGCGACGTACGGCGGTTGACCGACGGTCCGTGGATCGACACAATGCCGAGTTGGTCGCCGACCGGAGAATTCATAGCGTTTTCTTCAAACATGCATAACCCGAAAAATACCGAAGCATTTAGTATCTACGTCATTAAACCGGACGGTTCGGGTTTAAGGAGGGTGCACGTGGCTGGACCGGAAGGGTCTGATGAAGTGGACCGTGAGAGGATTAACCACGTGTGCTTCAGTAAAGATGGGGAGTGGCTTTTATTCACGTCAAATTTGGCTGGGGTGACGTCAGAGCCAGTTTCTTTGCCCAATCAGTTTCAGCCATATGGCGATTTGTATGTCGTGAGGTTGGATGGGACTGGGCTGAGGAGGCTGACGTGGAATGGATATGAGAACGGAACTCCCACGTGGCACTACGGGAGTGAGCTGGCGCTTTCTGGGCTGAGTTTGAAAGATGACGTGGTTGGAGAGAAGTTGGAGGGGAAGTTTGAAGAACCCCTTTGGATAACGTTCAAATGA

Protein sequence

MPFPKASSITLHFCTMDKNTPTGAVVFATVGRPQYGFDTFSVSLQSPTAEQRLTDGISVNFNAQFIDNQNSIVFISERSGSPKIYRSDSPNSAPELLPSATGSFFHDRPIIRNGRLFFTSAHENPHKSFASWSALYSSVLDGSDPVTRLTPPGFVDFSPAVSESGKFVAVASYGSRPWAGDFNELATEIVVFEADNPTRRVVVSGHGGWPSWCGDSAVYFHRTADDGWWSIFRVEIPENLESSVSSVPVRVTPAGLHCFTPAAMNDGKRVVVATRRPDDKFRHIEIFDSGSDEFDPITKKLNPGFHHYNPFVSPDSKSVGYHRFRGESTQGELTVPHLDSVISPINNLRMIRINGFFPTPSPDNDFIAYNPDFVGLKIVKSDSSKSWTVLKDRTAFYNSWSPTEKNVIFTSLGPIFGPARATVQIARITINYEDLNGGDEDEIDHEVKILTKEDTGNNAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGDVRRLTDGPWIDTMPSWSPTGEFIAFSSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPEGSDEVDRERINHVCFSKDGEWLLFTSNLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRRLTWNGYENGTPTWHYGSELALSGLSLKDDVVGEKLEGKFEEPLWITFK
Homology
BLAST of Sed0012789 vs. NCBI nr
Match: XP_022954120.1 (uncharacterized protein LOC111456480 [Cucurbita moschata])

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 527/641 (82.22%), Postives = 580/641 (90.48%), Query Frame = 0

Query: 21  PTGAVVFATVGRPQYGFDTFSVSLQSPTAEQRLTDGISVNFNAQFIDNQNSIVFISERSG 80
           PTG V+F TVGR QYGFDTFSV L SPT E  LTDGISVNFNAQF+DNQ SIVFISERSG
Sbjct: 4   PTGTVIFTTVGRTQYGFDTFSVPLSSPTTEHCLTDGISVNFNAQFVDNQQSIVFISERSG 63

Query: 81  SPKIYRSDSPNSAPELLPSATGSFFHDRPIIRNGRLFFTSAHENPHKSFASWSALYSSVL 140
           SP++Y S+SPNSAP+LLPSA GS FHDRPIIRN RL+F SAHENPHK F SWSALY + L
Sbjct: 64  SPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYFTGL 123

Query: 141 DGSDPVTRLTPPGFVDFSPAVSESGKFVAVASYGSRPWAGDFNELATEIVVFEADNPTRR 200
           DGSD VTRLTP G VDFSPAVSESGKFVAVASYGSR W G+F EL TEIVVF + +P RR
Sbjct: 124 DGSDSVTRLTPCGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDPDRR 183

Query: 201 VVVSGHGGWPSWCGDSAVYFHRTADDGWWSIFRVEIPENLESSVSSVPVRVTPAGLHCFT 260
           VVVSG GGWPSW GDS VYFHR A+DGWWSIFRVEIPENL+SSV  VP+RVTPAGLHCFT
Sbjct: 184 VVVSGRGGWPSWSGDSTVYFHRQAEDGWWSIFRVEIPENLDSSVPPVPIRVTPAGLHCFT 243

Query: 261 PAAMNDGKRVVVATRRPDDKFRHIEIFDSGSDEFDPITKKLNPGFHHYNPFVSPDSKSVG 320
           PAAMND KRVVVATRRPD+KFRHIEI++SG+DEFDPIT+KLNP FHHYNPFVSPDSK +G
Sbjct: 244 PAAMNDRKRVVVATRRPDNKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFVSPDSKYIG 303

Query: 321 YHRFRGESTQGELTVPHLDSVISPINNLRMIRINGFFPTPSPDNDFIAYNPDFVGLKIVK 380
           YHRFRGES+ GELT+PH + +ISPIN LR+IRING FPT SPD +FIA+NPDFVGLKIVK
Sbjct: 304 YHRFRGESSHGELTIPHFERIISPINELRLIRINGSFPTQSPDGNFIAFNPDFVGLKIVK 363

Query: 381 SDSSKSWTVLKDRTAFYNSWSPTEKNVIFTSLGPIFGPARATVQIARITINYEDLNGGDE 440
           +D SK WTVLKDRTAFYNSWSPTEKNVI++SLGPIFGPARATVQIAR TIN +DLN GD 
Sbjct: 364 ADGSKCWTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINSDDLNNGDS 423

Query: 441 DEIDHEVKILTKEDTGNNAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGDVRRLT 500
           DE+  EVKILTKEDTGNNAFPACSPDG+FLVFRSGRSGHKNLYI+DAVNG+ +G+ RRLT
Sbjct: 424 DEVAGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDFNGEARRLT 483

Query: 501 DGPWIDTMPSWSPTGEFIAFSSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPEGSDEVD 560
           DGPWIDTMPSWSP G+ IAFSSNMHNP+NTE FSIYVI+PDGS LRRVHVAGPEGS +VD
Sbjct: 484 DGPWIDTMPSWSPAGDLIAFSSNMHNPENTETFSIYVIRPDGSNLRRVHVAGPEGSSDVD 543

Query: 561 RERINHVCFSKDGEWLLFTSNLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRRLTWNGY 620
           +ERINHVCFS+DGEWLLFTSNL GV++EPVS+PNQFQPYGDL+VVRLDGTGLRRLTW+ Y
Sbjct: 544 KERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVRLDGTGLRRLTWSAY 603

Query: 621 ENGTPTWHYGSELALSGLSLKDDVVGEKLEGKFEEPLWITF 662
           ENGTPTW+YGSELALSGLSLKD+VVGEKL+G+F+EPLWITF
Sbjct: 604 ENGTPTWYYGSELALSGLSLKDEVVGEKLKGEFDEPLWITF 644

BLAST of Sed0012789 vs. NCBI nr
Match: KAG6575727.1 (hypothetical protein SDJN03_26366, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 526/641 (82.06%), Postives = 578/641 (90.17%), Query Frame = 0

Query: 21  PTGAVVFATVGRPQYGFDTFSVSLQSPTAEQRLTDGISVNFNAQFIDNQNSIVFISERSG 80
           PTG V+F TVGR QYGFDTFSV L SPT E  LTDGISVNFNAQF+DNQ SIVFISERSG
Sbjct: 4   PTGTVIFTTVGRTQYGFDTFSVPLSSPTTEHCLTDGISVNFNAQFVDNQQSIVFISERSG 63

Query: 81  SPKIYRSDSPNSAPELLPSATGSFFHDRPIIRNGRLFFTSAHENPHKSFASWSALYSSVL 140
           SP++Y S+SPNSAP+LLPSA GS FHDRPIIRN RL+F SAHENPHK F SWSALY + L
Sbjct: 64  SPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYFTGL 123

Query: 141 DGSDPVTRLTPPGFVDFSPAVSESGKFVAVASYGSRPWAGDFNELATEIVVFEADNPTRR 200
           DGSD VTRLTP G VDFSPAVSESGKFVAVASYGSR W G+F EL TEIVVF + +P RR
Sbjct: 124 DGSDSVTRLTPCGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDPDRR 183

Query: 201 VVVSGHGGWPSWCGDSAVYFHRTADDGWWSIFRVEIPENLESSVSSVPVRVTPAGLHCFT 260
           VVVSG GGWPSW GDS VYFHR A+DGWWSIFRVEIPENL+SSV  V VRVTPAGLHCFT
Sbjct: 184 VVVSGRGGWPSWSGDSTVYFHRQAEDGWWSIFRVEIPENLDSSVPPVAVRVTPAGLHCFT 243

Query: 261 PAAMNDGKRVVVATRRPDDKFRHIEIFDSGSDEFDPITKKLNPGFHHYNPFVSPDSKSVG 320
           PAAMND KRVVVATRRPD+KFRHIEI++SG+DEFDPIT+KLNP FHHYNPFVSPDS  +G
Sbjct: 244 PAAMNDRKRVVVATRRPDNKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFVSPDSNYIG 303

Query: 321 YHRFRGESTQGELTVPHLDSVISPINNLRMIRINGFFPTPSPDNDFIAYNPDFVGLKIVK 380
           YHRFRGES+ GELT+PH + +ISPIN LR+IRING FPT SPD +FIA+NPDFVGLKIVK
Sbjct: 304 YHRFRGESSHGELTIPHFERIISPINELRLIRINGSFPTQSPDGNFIAFNPDFVGLKIVK 363

Query: 381 SDSSKSWTVLKDRTAFYNSWSPTEKNVIFTSLGPIFGPARATVQIARITINYEDLNGGDE 440
           +D SK WTVLKDRTAFYNSWSPTEKNVI++SLGPIFGPARATVQIAR TIN +DLN GD 
Sbjct: 364 ADGSKCWTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINSDDLNNGDS 423

Query: 441 DEIDHEVKILTKEDTGNNAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGDVRRLT 500
           DE+  EVKILTKEDTGNNAFPACSPDG+FLVFRSGRSGHKNLYI+DAVNG+ +G+ RRLT
Sbjct: 424 DEVAGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDFNGEARRLT 483

Query: 501 DGPWIDTMPSWSPTGEFIAFSSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPEGSDEVD 560
           DGPWIDTMPSWSP G+ IAFSSNMHNP+NTE FSIYVI+PDGS LRRVHVAGPEGS +VD
Sbjct: 484 DGPWIDTMPSWSPAGDLIAFSSNMHNPENTETFSIYVIRPDGSNLRRVHVAGPEGSSDVD 543

Query: 561 RERINHVCFSKDGEWLLFTSNLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRRLTWNGY 620
           +ERINHVCFS+DGEWLLFTSNL GV++EPVS+PNQFQPYGDL+VVRLDGTGLRRLTW+ Y
Sbjct: 544 KERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVRLDGTGLRRLTWSAY 603

Query: 621 ENGTPTWHYGSELALSGLSLKDDVVGEKLEGKFEEPLWITF 662
           ENGTPTW+YGSELALSGLSLKD+VVGEKL+G+F+EPLWITF
Sbjct: 604 ENGTPTWYYGSELALSGLSLKDEVVGEKLKGEFDEPLWITF 644

BLAST of Sed0012789 vs. NCBI nr
Match: XP_023547812.1 (uncharacterized protein LOC111806666 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1127.1 bits (2914), Expect = 0.0e+00
Identity = 527/641 (82.22%), Postives = 578/641 (90.17%), Query Frame = 0

Query: 21  PTGAVVFATVGRPQYGFDTFSVSLQSPTAEQRLTDGISVNFNAQFIDNQNSIVFISERSG 80
           PTG V+F TVGR QYGFDTFSV L SPT E  LTDGISVNFNAQF+DNQ SIVFISERSG
Sbjct: 4   PTGTVIFTTVGRTQYGFDTFSVPLNSPTTEHCLTDGISVNFNAQFVDNQLSIVFISERSG 63

Query: 81  SPKIYRSDSPNSAPELLPSATGSFFHDRPIIRNGRLFFTSAHENPHKSFASWSALYSSVL 140
           SP++Y S+SPNSAP+LLPSA GS FHDRPIIRN RL+F SAHENPHK F SWSALY + L
Sbjct: 64  SPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYFTGL 123

Query: 141 DGSDPVTRLTPPGFVDFSPAVSESGKFVAVASYGSRPWAGDFNELATEIVVFEADNPTRR 200
           DGSD VTRLTP G VDFSPAVSESGKFVAVASYGSR W G+F EL TEIVVF + +P RR
Sbjct: 124 DGSDSVTRLTPRGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDPDRR 183

Query: 201 VVVSGHGGWPSWCGDSAVYFHRTADDGWWSIFRVEIPENLESSVSSVPVRVTPAGLHCFT 260
           VVVSG GGWPSW GDS VYFHR A+DGWWSIFRVEIPENL+SSV  VP+RVTPAGLHCFT
Sbjct: 184 VVVSGRGGWPSWSGDSTVYFHRQAEDGWWSIFRVEIPENLDSSVPPVPIRVTPAGLHCFT 243

Query: 261 PAAMNDGKRVVVATRRPDDKFRHIEIFDSGSDEFDPITKKLNPGFHHYNPFVSPDSKSVG 320
           PAAMND KRVVVATRRPD+KFRHIEI++SG+DEFDPIT+KLNP FHHYNPFVSPDS  +G
Sbjct: 244 PAAMNDRKRVVVATRRPDNKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFVSPDSNYIG 303

Query: 321 YHRFRGESTQGELTVPHLDSVISPINNLRMIRINGFFPTPSPDNDFIAYNPDFVGLKIVK 380
           YHRFRGES+ GELT+PH + +ISPIN LRMIRING FPT SPD +FIA+NPDFVGL+IVK
Sbjct: 304 YHRFRGESSHGELTIPHFERIISPINELRMIRINGSFPTQSPDGNFIAFNPDFVGLRIVK 363

Query: 381 SDSSKSWTVLKDRTAFYNSWSPTEKNVIFTSLGPIFGPARATVQIARITINYEDLNGGDE 440
           +D SK  TVLKDRTAFYNSWSPTEKNVI++SLGPIFGPARATVQIAR TIN +DLN GD 
Sbjct: 364 ADGSKCVTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINSDDLNNGDS 423

Query: 441 DEIDHEVKILTKEDTGNNAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGDVRRLT 500
           DE+  EVKILTKEDTGNNAFPACSPDG+FLVFRSGRSGHKNLYI+DAVNG+  G+ RRLT
Sbjct: 424 DEVAGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDFGGEARRLT 483

Query: 501 DGPWIDTMPSWSPTGEFIAFSSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPEGSDEVD 560
           DGPWIDTMPSWSP G+ IAFSSNMHNPKNTEAFSIYVI+PDGS LRRVHVAGPEGS +VD
Sbjct: 484 DGPWIDTMPSWSPAGDLIAFSSNMHNPKNTEAFSIYVIRPDGSNLRRVHVAGPEGSSDVD 543

Query: 561 RERINHVCFSKDGEWLLFTSNLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRRLTWNGY 620
           +ERINHVCFS+DGEWLLFTSNL GV++EPVS+PNQFQPYGDL+VVRLDGTGLRRLTW+ Y
Sbjct: 544 KERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVRLDGTGLRRLTWSAY 603

Query: 621 ENGTPTWHYGSELALSGLSLKDDVVGEKLEGKFEEPLWITF 662
           ENGTPTW+YGSELALSGLSLKD+VVGEKL+G+F+EPLWITF
Sbjct: 604 ENGTPTWYYGSELALSGLSLKDEVVGEKLKGEFDEPLWITF 644

BLAST of Sed0012789 vs. NCBI nr
Match: KAG7014283.1 (tolB, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1123.2 bits (2904), Expect = 0.0e+00
Identity = 524/641 (81.75%), Postives = 576/641 (89.86%), Query Frame = 0

Query: 21  PTGAVVFATVGRPQYGFDTFSVSLQSPTAEQRLTDGISVNFNAQFIDNQNSIVFISERSG 80
           PTG V+F TVGR QYGFDTFSV L SPT E  LTDGISVNFNAQF+DNQ SIVFISERSG
Sbjct: 4   PTGTVIFTTVGRTQYGFDTFSVPLSSPTTEHCLTDGISVNFNAQFVDNQQSIVFISERSG 63

Query: 81  SPKIYRSDSPNSAPELLPSATGSFFHDRPIIRNGRLFFTSAHENPHKSFASWSALYSSVL 140
           SP++Y S+SPNSAP+LLPSA GS FHDRPIIRN RL+F SAHENPHK F SWSALY + L
Sbjct: 64  SPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYFTGL 123

Query: 141 DGSDPVTRLTPPGFVDFSPAVSESGKFVAVASYGSRPWAGDFNELATEIVVFEADNPTRR 200
           DGSD VTRLTP G VDFSPAVSESGKFVAVASYGSR W G+F EL TEIVVF + +P RR
Sbjct: 124 DGSDSVTRLTPCGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDPDRR 183

Query: 201 VVVSGHGGWPSWCGDSAVYFHRTADDGWWSIFRVEIPENLESSVSSVPVRVTPAGLHCFT 260
           VVVSG GGWPSW GDS VYFHR A+DGWWSIF VEIPENL+SSV  V VRVTPAGLHCFT
Sbjct: 184 VVVSGRGGWPSWSGDSTVYFHRQAEDGWWSIFSVEIPENLDSSVPPVAVRVTPAGLHCFT 243

Query: 261 PAAMNDGKRVVVATRRPDDKFRHIEIFDSGSDEFDPITKKLNPGFHHYNPFVSPDSKSVG 320
           PAAMND KRVVVATRRPD+KFRHIEI++SG+DEFDPIT+KLNP FHHYNPFVSPDS  +G
Sbjct: 244 PAAMNDRKRVVVATRRPDNKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFVSPDSNYIG 303

Query: 321 YHRFRGESTQGELTVPHLDSVISPINNLRMIRINGFFPTPSPDNDFIAYNPDFVGLKIVK 380
           YHRFRGES+ GELT+PH + +ISPIN LR+IRING FPT SPD +FIA+NPDFVGLKIVK
Sbjct: 304 YHRFRGESSHGELTIPHFERIISPINELRLIRINGSFPTQSPDGNFIAFNPDFVGLKIVK 363

Query: 381 SDSSKSWTVLKDRTAFYNSWSPTEKNVIFTSLGPIFGPARATVQIARITINYEDLNGGDE 440
           +D SK WTVLKDRTAFYNSWSPTEKNVI++SLGPIFGPARATVQIAR TIN +DLN GD 
Sbjct: 364 ADGSKCWTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINSDDLNNGDS 423

Query: 441 DEIDHEVKILTKEDTGNNAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGDVRRLT 500
           DE+  EVKILTKEDTGNNAFPACSPDG+FLVFRSGRSGHKNLYI+DAVNG+ +G+ RRLT
Sbjct: 424 DEVAGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDFNGEARRLT 483

Query: 501 DGPWIDTMPSWSPTGEFIAFSSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPEGSDEVD 560
           DGPWIDTMPSWSP G+ IAFSSNMHNP+NTE FSIYVI+PDGS LRRVHVAGPEGS +VD
Sbjct: 484 DGPWIDTMPSWSPAGDLIAFSSNMHNPENTETFSIYVIRPDGSNLRRVHVAGPEGSSDVD 543

Query: 561 RERINHVCFSKDGEWLLFTSNLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRRLTWNGY 620
           +ERINHVCFS+DGEWLLFTSNL GV++EPVS+PNQFQPYGDL+VV+LDGTGLRRLTW+ Y
Sbjct: 544 KERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVKLDGTGLRRLTWSAY 603

Query: 621 ENGTPTWHYGSELALSGLSLKDDVVGEKLEGKFEEPLWITF 662
           ENGTPTW+YGSELALSGLSLKD VVGEKL+G+F+EPLWITF
Sbjct: 604 ENGTPTWYYGSELALSGLSLKDAVVGEKLKGEFDEPLWITF 644

BLAST of Sed0012789 vs. NCBI nr
Match: XP_022991715.1 (uncharacterized protein LOC111488250 [Cucurbita maxima])

HSP 1 Score: 1119.8 bits (2895), Expect = 0.0e+00
Identity = 519/641 (80.97%), Postives = 577/641 (90.02%), Query Frame = 0

Query: 21  PTGAVVFATVGRPQYGFDTFSVSLQSPTAEQRLTDGISVNFNAQFIDNQNSIVFISERSG 80
           PTG V+F TVGR QYGFDTFSV L SPT E  LTDGISVNFNAQF+DNQ SIVFISERSG
Sbjct: 4   PTGTVIFTTVGRTQYGFDTFSVPLSSPTTEHCLTDGISVNFNAQFVDNQQSIVFISERSG 63

Query: 81  SPKIYRSDSPNSAPELLPSATGSFFHDRPIIRNGRLFFTSAHENPHKSFASWSALYSSVL 140
           SP++Y S+SPNSAP+LLPSA GS FHDRPIIRN RL+F SAHENPHK F SWSALY + L
Sbjct: 64  SPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYVTGL 123

Query: 141 DGSDPVTRLTPPGFVDFSPAVSESGKFVAVASYGSRPWAGDFNELATEIVVFEADNPTRR 200
           DGS  VTRLTP G VDFSPAVSESGKFVAVASYGSR W G+F EL TEIVVF + +P RR
Sbjct: 124 DGSVSVTRLTPRGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDPDRR 183

Query: 201 VVVSGHGGWPSWCGDSAVYFHRTADDGWWSIFRVEIPENLESSVSSVPVRVTPAGLHCFT 260
           V+VSG GGWPSW GDS V+FHR A+DGWWSIFR EIPENL+SSV  VP+RVTPAGLHCFT
Sbjct: 184 VIVSGRGGWPSWSGDSTVFFHRQAEDGWWSIFRGEIPENLDSSVPPVPIRVTPAGLHCFT 243

Query: 261 PAAMNDGKRVVVATRRPDDKFRHIEIFDSGSDEFDPITKKLNPGFHHYNPFVSPDSKSVG 320
           PAAMND KRVVVATRRPD+KFRHIEI++SG+DEFDPIT+KLNP FHHYNPFVSPDS  +G
Sbjct: 244 PAAMNDRKRVVVATRRPDNKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFVSPDSNYIG 303

Query: 321 YHRFRGESTQGELTVPHLDSVISPINNLRMIRINGFFPTPSPDNDFIAYNPDFVGLKIVK 380
           YHRFRGES+ GE+T+PH + +ISPIN LR+IRING FPT SPD +FIA+NPDFVGLKIVK
Sbjct: 304 YHRFRGESSHGEITIPHFERIISPINELRLIRINGSFPTQSPDGNFIAFNPDFVGLKIVK 363

Query: 381 SDSSKSWTVLKDRTAFYNSWSPTEKNVIFTSLGPIFGPARATVQIARITINYEDLNGGDE 440
           +D SK WTVLKDRTAFYNSWSPTEKNVI++SLGPIFGPARATVQIAR TIN +DLN GD 
Sbjct: 364 ADGSKCWTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINSDDLNNGDN 423

Query: 441 DEIDHEVKILTKEDTGNNAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGDVRRLT 500
           DE+  EVKILTKEDTGNNAFPACSPDG+FLVFRSGRSGHKNLYI+DAVNG+ +G+ R+LT
Sbjct: 424 DEVSGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDFNGEARQLT 483

Query: 501 DGPWIDTMPSWSPTGEFIAFSSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPEGSDEVD 560
           +GPWIDTMPSWSP G+ IAFSSNMHNP+NTE FSIYVI+PDGS LRRVHVAGPEGS +VD
Sbjct: 484 NGPWIDTMPSWSPAGDLIAFSSNMHNPENTETFSIYVIRPDGSNLRRVHVAGPEGSSDVD 543

Query: 561 RERINHVCFSKDGEWLLFTSNLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRRLTWNGY 620
           +ERINHVCFS+DGEWLLFTSNL GV++EPVS+PNQFQPYGDL+VVRLDGTGLRRLTW+ Y
Sbjct: 544 KERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVRLDGTGLRRLTWSAY 603

Query: 621 ENGTPTWHYGSELALSGLSLKDDVVGEKLEGKFEEPLWITF 662
           ENGTPTW+YGSELALSGLSLKD+VVGEKL+G+F+EPLWITF
Sbjct: 604 ENGTPTWYYGSELALSGLSLKDEVVGEKLKGEFDEPLWITF 644

BLAST of Sed0012789 vs. ExPASy Swiss-Prot
Match: Q3APB5 (Protein TolB homolog OS=Chlorobium chlorochromatii (strain CaD3) OX=340177 GN=tolB PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 5.5e-15
Identity = 78/290 (26.90%), Postives = 118/290 (40.69%), Query Frame = 0

Query: 342 ISPINNLRMIRINGFFPTPSPDNDFIAYNPDFVGLKIVKSDSSKSWTVLKDRTAFYNSWS 401
           +  + N R I +    P  SPD  ++A+  DF G         K    +++         
Sbjct: 201 VHQLTNFRSIALT---PALSPDGAYLAFT-DFTG--------GKPALAIRELA------- 260

Query: 402 PTEKNVIFTSLGPIFGPARATVQIARITINYEDLNGGDED----EIDHEVKILTKEDTGN 461
            T K       G    PA    +    T ++E    GD++    +    VK      +G 
Sbjct: 261 -TGKTTRVAKKGNSIDPAWRNSRELATTFSFE----GDQELYLLDSAGAVKQRLTSSSGI 320

Query: 462 NAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGDVRRLTDGPWIDTMPSWSPTGEF 521
           +  P  SPDGR + F S RSG+  +++ D  +G+     +RLT     +T P+WSP G+ 
Sbjct: 321 DLSPTFSPDGRKMAFVSARSGNPQIFVYDFSSGKS----QRLTFSGRYNTQPAWSPIGDK 380

Query: 522 IAFSSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPEGSDEVDRERINHVCFSKDGEWLL 581
           IAFS+     ++    +I+VI  DGSGL ++     E              +S DG  ++
Sbjct: 381 IAFST----WESGGEINIFVINTDGSGLTQLTTQSGENESP---------SWSPDGRMIV 435

Query: 582 FTSNLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRRLTWNGYENGTPTW 628
           F SN  GV                LYV+  DG   R L   G E   P+W
Sbjct: 441 FASNRQGVKK--------------LYVMMADGKNQRPLLAIGGEQTQPSW 435

BLAST of Sed0012789 vs. ExPASy Swiss-Prot
Match: Q3A097 (Tol-Pal system protein TolB OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1) OX=338963 GN=tolB PE=3 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 1.6e-14
Identity = 78/296 (26.35%), Postives = 128/296 (43.24%), Query Frame = 0

Query: 362 PDNDFIAYNPDFVG---LKIVKSDSSKSWTVLKDRTAFYN-SWSPTEKNVIFTSL----G 421
           P +  IAY  +  G   L ++ +D + +  +   R+   N  +SP  + VI+TS      
Sbjct: 164 PFSSRIAYIDNRTGHKELYLMDTDGANALRLTDHRSIVLNPDFSPNGREVIYTSYRRGNP 223

Query: 422 PIFGPARATVQIARITINYEDLNGGDEDEIDHEVKILTKEDTGN---------------- 481
            ++    +T Q AR+    + LN       D     LT+  TGN                
Sbjct: 224 DLYRKQLSTGQEARLAFK-KGLNIAARFRPDGREIALTQSATGNPELMLLGTDGSRRRRL 283

Query: 482 ------NAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGDVRRLTDGPWIDTMPSW 541
                 +  P+ SP G  L F S R G+ +++++D + G+      RLT     +  P+W
Sbjct: 284 TSHWGIDVDPSWSPAGDRLAFVSDRQGNPHIFVMDLLGGQ----TARLTANGKYNATPAW 343

Query: 542 SPTGEFIAFSSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPEGSDEVDRERINHVCFSK 601
           SP G+ IAF+           F IY ++PDG+  RR+   GP G+ E       H  +S 
Sbjct: 344 SPDGKRIAFTR-----LEKGVFDIYTVRPDGTDERRL-TFGP-GNKE-------HPRWSP 403

Query: 602 DGEWLLFTSNLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRRLTWNGYENGTPTW 628
           D  +L+++S+  G                 +Y++R DGTG+RR++  G +   P W
Sbjct: 404 DSRFLVYSSDQDGRKG--------------IYIMRADGTGIRRISAGGGQCQHPAW 426

BLAST of Sed0012789 vs. ExPASy Swiss-Prot
Match: Q0AC40 (Tol-Pal system protein TolB OS=Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1) OX=187272 GN=tolB PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 6.7e-13
Identity = 82/311 (26.37%), Postives = 123/311 (39.55%), Query Frame = 0

Query: 324 FRGESTQGELTVPHLDSVISPINNLRMIRINGFFPTPSPDNDFIAY-------NPDFVGL 383
           FRG   +G     H D++ S  + +  I        P      IAY       +     L
Sbjct: 124 FRGNRIEGRRYRLHPDNLRSLAHAISDIVFEEVVGLPGVFGTQIAYVQVEERNDQRIHRL 183

Query: 384 KIVKSDSSKSWTVLKDRTAFYN-SWSPTEKNVIFTSLGPIFGPARATVQIARITINYEDL 443
            +  +D  +   +L+ R    + +WSP    + + S            +  R  I  ++L
Sbjct: 184 MVADADGQRPREILESRQPIMSPAWSPERDRLAYVSF-----------EGGRSEIYVQNL 243

Query: 444 NGGDEDEIDHEVKILTKEDTGNNAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGD 503
             G  D I            G N+ PA SPDGR L     R G  N+Y++       DG 
Sbjct: 244 RSGQRDRI--------ASFRGINSAPAWSPDGRHLAVTLSRDGRANIYLLRL----DDGH 303

Query: 504 VRRLTDGPWIDTMPSWSPTGEFIAFSSNMHNPKNTEAFSIYVIKPDG-SGLRRV-----H 563
           VRRLTD   IDT P++SP GE IAF+S+           +Y +  +G  G+ RV     +
Sbjct: 304 VRRLTDHWAIDTEPTFSPDGERIAFTSDRGGRP-----QVYTLAVNGPGGVERVTFDGDY 363

Query: 564 VAGPEGSDEVDRERINHVCFSKDGEWLLFTSNLAG-----VTSEPVSLPNQFQPYGDLYV 616
            A P  S +  R  + H     +G + +   +L       +T  P      F P GD+ +
Sbjct: 364 NARPNWSPDGRRIAMVH---RHNGSFRIAVHDLESDRTRVLTDGPWDESPVFAPNGDMIM 403

BLAST of Sed0012789 vs. ExPASy Swiss-Prot
Match: A8LHQ6 (Tol-Pal system protein TolB OS=Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12) OX=398580 GN=tolB PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.1e-12
Identity = 54/167 (32.34%), Postives = 77/167 (46.11%), Query Frame = 0

Query: 461 PACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGDVRRLTDGPWIDTMPSWSPTGEFIAF 520
           P+ SPDGR +VF S RSG + LY++ A  GE     RR++ GP     P WSP G+ IAF
Sbjct: 306 PSFSPDGRQIVFESDRSGSQQLYVMSATGGE----ARRISFGPGRYGTPVWSPRGDLIAF 365

Query: 521 SSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPEGSDEVDRERINHVCFSKDGEWLLFTS 580
           +      +N   F I V++ DGS  R +  +  + S            +S +G  ++FT 
Sbjct: 366 TK-----QNQGRFHIGVMRTDGSEERLLTSSFLDESP----------TWSPNGRVIMFTR 425

Query: 581 NLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRRLTWNGYENGTPTW 628
             +G    P            LY V + G  LRR+   G  +  P W
Sbjct: 426 ETSGAGGAP-----------SLYSVDISGRNLRRVPTPGAAS-DPAW 441

BLAST of Sed0012789 vs. ExPASy Swiss-Prot
Match: Q9A3H4 (Tol-Pal system protein TolB OS=Caulobacter vibrioides (strain ATCC 19089 / CB15) OX=190650 GN=tolB PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 6.3e-11
Identity = 53/167 (31.74%), Postives = 78/167 (46.71%), Query Frame = 0

Query: 461 PACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGDVRRLTDGPWIDTMPSWSPTGEFIAF 520
           P  SPDG  + F   R+G+ ++Y++D           R+T  P IDT PS+SP G  I F
Sbjct: 250 PRFSPDGSKVAFSVERNGNSDIYVMDL----RSRATTRITTDPAIDTSPSFSPDGTKIVF 309

Query: 521 SSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPEGSDEVDRERINHVCFSKDGEWLLFTS 580
           +S+    +  +A  IYV+  DGSG+RR+   G   +  V         +S  G+++ FT 
Sbjct: 310 NSD----RGGQA-QIYVMNTDGSGVRRISYGGGRYTTPV---------WSPRGDFIAFTK 369

Query: 581 NLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRRLTWNGYENGTPTW 628
              G          +F     + V+R DG G  RL    Y +  PTW
Sbjct: 370 QTGG----------EFH----IGVMRADG-GDERLLTTSYLDEGPTW 383

BLAST of Sed0012789 vs. ExPASy TrEMBL
Match: A0A6J1GRK1 (uncharacterized protein LOC111456480 OS=Cucurbita moschata OX=3662 GN=LOC111456480 PE=4 SV=1)

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 527/641 (82.22%), Postives = 580/641 (90.48%), Query Frame = 0

Query: 21  PTGAVVFATVGRPQYGFDTFSVSLQSPTAEQRLTDGISVNFNAQFIDNQNSIVFISERSG 80
           PTG V+F TVGR QYGFDTFSV L SPT E  LTDGISVNFNAQF+DNQ SIVFISERSG
Sbjct: 4   PTGTVIFTTVGRTQYGFDTFSVPLSSPTTEHCLTDGISVNFNAQFVDNQQSIVFISERSG 63

Query: 81  SPKIYRSDSPNSAPELLPSATGSFFHDRPIIRNGRLFFTSAHENPHKSFASWSALYSSVL 140
           SP++Y S+SPNSAP+LLPSA GS FHDRPIIRN RL+F SAHENPHK F SWSALY + L
Sbjct: 64  SPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYFTGL 123

Query: 141 DGSDPVTRLTPPGFVDFSPAVSESGKFVAVASYGSRPWAGDFNELATEIVVFEADNPTRR 200
           DGSD VTRLTP G VDFSPAVSESGKFVAVASYGSR W G+F EL TEIVVF + +P RR
Sbjct: 124 DGSDSVTRLTPCGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDPDRR 183

Query: 201 VVVSGHGGWPSWCGDSAVYFHRTADDGWWSIFRVEIPENLESSVSSVPVRVTPAGLHCFT 260
           VVVSG GGWPSW GDS VYFHR A+DGWWSIFRVEIPENL+SSV  VP+RVTPAGLHCFT
Sbjct: 184 VVVSGRGGWPSWSGDSTVYFHRQAEDGWWSIFRVEIPENLDSSVPPVPIRVTPAGLHCFT 243

Query: 261 PAAMNDGKRVVVATRRPDDKFRHIEIFDSGSDEFDPITKKLNPGFHHYNPFVSPDSKSVG 320
           PAAMND KRVVVATRRPD+KFRHIEI++SG+DEFDPIT+KLNP FHHYNPFVSPDSK +G
Sbjct: 244 PAAMNDRKRVVVATRRPDNKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFVSPDSKYIG 303

Query: 321 YHRFRGESTQGELTVPHLDSVISPINNLRMIRINGFFPTPSPDNDFIAYNPDFVGLKIVK 380
           YHRFRGES+ GELT+PH + +ISPIN LR+IRING FPT SPD +FIA+NPDFVGLKIVK
Sbjct: 304 YHRFRGESSHGELTIPHFERIISPINELRLIRINGSFPTQSPDGNFIAFNPDFVGLKIVK 363

Query: 381 SDSSKSWTVLKDRTAFYNSWSPTEKNVIFTSLGPIFGPARATVQIARITINYEDLNGGDE 440
           +D SK WTVLKDRTAFYNSWSPTEKNVI++SLGPIFGPARATVQIAR TIN +DLN GD 
Sbjct: 364 ADGSKCWTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINSDDLNNGDS 423

Query: 441 DEIDHEVKILTKEDTGNNAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGDVRRLT 500
           DE+  EVKILTKEDTGNNAFPACSPDG+FLVFRSGRSGHKNLYI+DAVNG+ +G+ RRLT
Sbjct: 424 DEVAGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDFNGEARRLT 483

Query: 501 DGPWIDTMPSWSPTGEFIAFSSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPEGSDEVD 560
           DGPWIDTMPSWSP G+ IAFSSNMHNP+NTE FSIYVI+PDGS LRRVHVAGPEGS +VD
Sbjct: 484 DGPWIDTMPSWSPAGDLIAFSSNMHNPENTETFSIYVIRPDGSNLRRVHVAGPEGSSDVD 543

Query: 561 RERINHVCFSKDGEWLLFTSNLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRRLTWNGY 620
           +ERINHVCFS+DGEWLLFTSNL GV++EPVS+PNQFQPYGDL+VVRLDGTGLRRLTW+ Y
Sbjct: 544 KERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVRLDGTGLRRLTWSAY 603

Query: 621 ENGTPTWHYGSELALSGLSLKDDVVGEKLEGKFEEPLWITF 662
           ENGTPTW+YGSELALSGLSLKD+VVGEKL+G+F+EPLWITF
Sbjct: 604 ENGTPTWYYGSELALSGLSLKDEVVGEKLKGEFDEPLWITF 644

BLAST of Sed0012789 vs. ExPASy TrEMBL
Match: A0A6J1JTR3 (uncharacterized protein LOC111488250 OS=Cucurbita maxima OX=3661 GN=LOC111488250 PE=4 SV=1)

HSP 1 Score: 1119.8 bits (2895), Expect = 0.0e+00
Identity = 519/641 (80.97%), Postives = 577/641 (90.02%), Query Frame = 0

Query: 21  PTGAVVFATVGRPQYGFDTFSVSLQSPTAEQRLTDGISVNFNAQFIDNQNSIVFISERSG 80
           PTG V+F TVGR QYGFDTFSV L SPT E  LTDGISVNFNAQF+DNQ SIVFISERSG
Sbjct: 4   PTGTVIFTTVGRTQYGFDTFSVPLSSPTTEHCLTDGISVNFNAQFVDNQQSIVFISERSG 63

Query: 81  SPKIYRSDSPNSAPELLPSATGSFFHDRPIIRNGRLFFTSAHENPHKSFASWSALYSSVL 140
           SP++Y S+SPNSAP+LLPSA GS FHDRPIIRN RL+F SAHENPHK F SWSALY + L
Sbjct: 64  SPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYVTGL 123

Query: 141 DGSDPVTRLTPPGFVDFSPAVSESGKFVAVASYGSRPWAGDFNELATEIVVFEADNPTRR 200
           DGS  VTRLTP G VDFSPAVSESGKFVAVASYGSR W G+F EL TEIVVF + +P RR
Sbjct: 124 DGSVSVTRLTPRGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDPDRR 183

Query: 201 VVVSGHGGWPSWCGDSAVYFHRTADDGWWSIFRVEIPENLESSVSSVPVRVTPAGLHCFT 260
           V+VSG GGWPSW GDS V+FHR A+DGWWSIFR EIPENL+SSV  VP+RVTPAGLHCFT
Sbjct: 184 VIVSGRGGWPSWSGDSTVFFHRQAEDGWWSIFRGEIPENLDSSVPPVPIRVTPAGLHCFT 243

Query: 261 PAAMNDGKRVVVATRRPDDKFRHIEIFDSGSDEFDPITKKLNPGFHHYNPFVSPDSKSVG 320
           PAAMND KRVVVATRRPD+KFRHIEI++SG+DEFDPIT+KLNP FHHYNPFVSPDS  +G
Sbjct: 244 PAAMNDRKRVVVATRRPDNKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFVSPDSNYIG 303

Query: 321 YHRFRGESTQGELTVPHLDSVISPINNLRMIRINGFFPTPSPDNDFIAYNPDFVGLKIVK 380
           YHRFRGES+ GE+T+PH + +ISPIN LR+IRING FPT SPD +FIA+NPDFVGLKIVK
Sbjct: 304 YHRFRGESSHGEITIPHFERIISPINELRLIRINGSFPTQSPDGNFIAFNPDFVGLKIVK 363

Query: 381 SDSSKSWTVLKDRTAFYNSWSPTEKNVIFTSLGPIFGPARATVQIARITINYEDLNGGDE 440
           +D SK WTVLKDRTAFYNSWSPTEKNVI++SLGPIFGPARATVQIAR TIN +DLN GD 
Sbjct: 364 ADGSKCWTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINSDDLNNGDN 423

Query: 441 DEIDHEVKILTKEDTGNNAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGDVRRLT 500
           DE+  EVKILTKEDTGNNAFPACSPDG+FLVFRSGRSGHKNLYI+DAVNG+ +G+ R+LT
Sbjct: 424 DEVSGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDFNGEARQLT 483

Query: 501 DGPWIDTMPSWSPTGEFIAFSSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPEGSDEVD 560
           +GPWIDTMPSWSP G+ IAFSSNMHNP+NTE FSIYVI+PDGS LRRVHVAGPEGS +VD
Sbjct: 484 NGPWIDTMPSWSPAGDLIAFSSNMHNPENTETFSIYVIRPDGSNLRRVHVAGPEGSSDVD 543

Query: 561 RERINHVCFSKDGEWLLFTSNLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRRLTWNGY 620
           +ERINHVCFS+DGEWLLFTSNL GV++EPVS+PNQFQPYGDL+VVRLDGTGLRRLTW+ Y
Sbjct: 544 KERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVRLDGTGLRRLTWSAY 603

Query: 621 ENGTPTWHYGSELALSGLSLKDDVVGEKLEGKFEEPLWITF 662
           ENGTPTW+YGSELALSGLSLKD+VVGEKL+G+F+EPLWITF
Sbjct: 604 ENGTPTWYYGSELALSGLSLKDEVVGEKLKGEFDEPLWITF 644

BLAST of Sed0012789 vs. ExPASy TrEMBL
Match: A0A5A7URS2 (TolB protein-related isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00020 PE=4 SV=1)

HSP 1 Score: 1072.8 bits (2773), Expect = 4.9e-310
Identity = 508/646 (78.64%), Postives = 568/646 (87.93%), Query Frame = 0

Query: 19  NTPTGAVVFATVGRPQYGFDTFSVSLQSPTAEQRLTDGISVNFNAQFIDNQNSIVFISER 78
           + PTGAV+F T+G  QYGFD FSV+L SPT E+RLTDGISVNFNAQF++NQ S+VFISER
Sbjct: 2   DNPTGAVLFTTIGLNQYGFDIFSVALNSPTVERRLTDGISVNFNAQFLNNQLSVVFISER 61

Query: 79  SGSPKIYRSDSPNSAPELLPSATGSFFHDRPIIRNGRLFFTSAHENPHKSFASWSALYSS 138
           SGS +IY SDSPNS+P+LL SA GS FHDRPI+RNGRL F SAHENPHK F SW+ALYS+
Sbjct: 62  SGSSRIYLSDSPNSSPKLLASAPGSCFHDRPIVRNGRLLFISAHENPHKPFTSWAALYST 121

Query: 139 VLDGSDPVTRLTPPGFVDFSPAVSESGKFVAVASYGSRPWAGDFNELATEIVVFEADNPT 198
            LDG D +TRLTP G VDFSPAVSESGKFVAVASYGSR W G+F+EL  EIVVF++ +P 
Sbjct: 122 PLDGDDSITRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNLEIVVFKSSDPD 181

Query: 199 RRVVVSGHGGWPSWCGDSAVYFHRTADDGWWSIFRVEIPENLESSVSS---VPVRVTPAG 258
           RRVVV+  GGWPSW GDS V+FHR A+DGWWSIF+VEIPENL+SS+SS   VP+RVTPAG
Sbjct: 182 RRVVVASRGGWPSWSGDSTVFFHRKAEDGWWSIFKVEIPENLDSSMSSDSPVPIRVTPAG 241

Query: 259 LHCFTPAAMNDGKRVVVATRRPDDKFRHIEIFDSGSDEFDPITKKLNPGFHHYNPFVSPD 318
           LHCFTPAAMND + VVVATRR D KFRHIEIFDS  +EF PIT+KLNP FHHYNPFVSPD
Sbjct: 242 LHCFTPAAMNDSRLVVVATRRADSKFRHIEIFDSELEEFIPITQKLNPDFHHYNPFVSPD 301

Query: 319 SKSVGYHRFRGESTQGELTVPHLDSVISPINNLRMIRINGFFPTPSPDNDFIAYNPDFVG 378
           S  +GYHRFRGESTQ EL +P+L  VISPI  L+MIRING FPTPSPD D IA+NP+F G
Sbjct: 302 SNFIGYHRFRGESTQSEL-IPYLSPVISPIKELQMIRINGSFPTPSPDGDLIAFNPNFNG 361

Query: 379 LKIVKSDSSKSWTVLKDRTAFYNSWSPTEKNVIFTSLGPIFGPARATVQIARITINYEDL 438
           L+IVK D SK  TVLKDRTAFYNSWSPTEKNVI+TSLGPIFG   ATVQIARITIN +DL
Sbjct: 362 LQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVTATVQIARITINSDDL 421

Query: 439 NGGDEDEIDHEVKILTKEDTGNNAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGD 498
             GD DE+  EVKILTK+DTGNNAFPACSPDG+FLVFRSGRSGHKNLYIVDAV GE +G+
Sbjct: 422 KDGDSDEVSSEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVKGEFEGE 481

Query: 499 VRRLTDGPWIDTMPSWSPTGEFIAFSSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPEG 558
           +RRLTDG WIDTMP+WSP G+ I FSSNMHNPKNTEAFSIYVI+PDGSGLRRV+VAGPEG
Sbjct: 482 LRRLTDGAWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYVAGPEG 541

Query: 559 SDEVDRERINHVCFSKDGEWLLFTSNLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRRL 618
           S EVDRERINHVCFS+DG+WLLFT+NL+GVT+EPVS PNQFQPYGDL+VVRLDGTGLRRL
Sbjct: 542 SSEVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSWPNQFQPYGDLFVVRLDGTGLRRL 601

Query: 619 TWNGYENGTPTWHYGSELALSGLSLKDDVVGEKLEGKFEEPLWITF 662
           TWNGYENGTPTW+YGSE+ALSGLSLKD+VVGEKL+G F+EPLWITF
Sbjct: 602 TWNGYENGTPTWYYGSEVALSGLSLKDEVVGEKLKGNFDEPLWITF 646

BLAST of Sed0012789 vs. ExPASy TrEMBL
Match: A0A1S3CEJ8 (uncharacterized protein LOC103499982 OS=Cucumis melo OX=3656 GN=LOC103499982 PE=4 SV=1)

HSP 1 Score: 1065.8 bits (2755), Expect = 7.1e-308
Identity = 505/646 (78.17%), Postives = 565/646 (87.46%), Query Frame = 0

Query: 19  NTPTGAVVFATVGRPQYGFDTFSVSLQSPTAEQRLTDGISVNFNAQFIDNQNSIVFISER 78
           + PTGAV+F T+G  QYGFD FSV+L SPT E+RLTDGISVNFNAQF++NQ S+VFISER
Sbjct: 2   DNPTGAVLFTTIGLNQYGFDIFSVALNSPTVERRLTDGISVNFNAQFLNNQLSVVFISER 61

Query: 79  SGSPKIYRSDSPNSAPELLPSATGSFFHDRPIIRNGRLFFTSAHENPHKSFASWSALYSS 138
           SGS +IY SDSPNS+P+LL SA GS FHDRPI+RNGRL F SAHENPHK F SW+ALYS+
Sbjct: 62  SGSSRIYLSDSPNSSPKLLASAPGSCFHDRPIVRNGRLLFISAHENPHKPFTSWAALYST 121

Query: 139 VLDGSDPVTRLTPPGFVDFSPAVSESGKFVAVASYGSRPWAGDFNELATEIVVFEADNPT 198
            LDG D +TRLTP G VDFSPAVS SGKFVAVASYGSR W G+F+EL  EIVVF++ +P 
Sbjct: 122 PLDGDDSITRLTPLGSVDFSPAVSGSGKFVAVASYGSRSWGGEFHELNLEIVVFKSSDPD 181

Query: 199 RRVVVSGHGGWPSWCGDSAVYFHRTADDGWWSIFRVEIPENLESSVSS---VPVRVTPAG 258
           RRVVV+  GGWPSW GDS V+FHR A+DGWWSIF+VEIPENL+SS+SS   VP+RVTPAG
Sbjct: 182 RRVVVASRGGWPSWSGDSTVFFHRKAEDGWWSIFKVEIPENLDSSMSSDSPVPIRVTPAG 241

Query: 259 LHCFTPAAMNDGKRVVVATRRPDDKFRHIEIFDSGSDEFDPITKKLNPGFHHYNPFVSPD 318
           LHCFTPAAMND + VVVATRR D KFRHIEIFDS  +EF PIT+KLNP FHHYNPFVSPD
Sbjct: 242 LHCFTPAAMNDSRLVVVATRRADSKFRHIEIFDSELEEFIPITQKLNPDFHHYNPFVSPD 301

Query: 319 SKSVGYHRFRGESTQGELTVPHLDSVISPINNLRMIRINGFFPTPSPDNDFIAYNPDFVG 378
           S  +GYHRFRGESTQ EL +P+L  VISPI  L+MIRING FP PSPD D IA+NP+F G
Sbjct: 302 SNFIGYHRFRGESTQSEL-IPYLSPVISPIKELQMIRINGSFPAPSPDGDLIAFNPNFNG 361

Query: 379 LKIVKSDSSKSWTVLKDRTAFYNSWSPTEKNVIFTSLGPIFGPARATVQIARITINYEDL 438
           L+IVK D SK  TVLKDRTAFYNSWSPTEKNVI+TSLGPIFG   ATVQIARITIN +DL
Sbjct: 362 LQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVTATVQIARITINSDDL 421

Query: 439 NGGDEDEIDHEVKILTKEDTGNNAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGD 498
             GD DE+  EVKILTK+DTGNNAFPACSPDG+FLVFRSGRSGHKNLYIVDAV GE +G+
Sbjct: 422 KDGDSDEVSSEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVKGEFEGE 481

Query: 499 VRRLTDGPWIDTMPSWSPTGEFIAFSSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPEG 558
           +RRLTDG WIDTMP+WSP G+ I FSSNMHNPKNTEAFSIYVI+PDGSGLRRV+VAGPEG
Sbjct: 482 LRRLTDGAWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYVAGPEG 541

Query: 559 SDEVDRERINHVCFSKDGEWLLFTSNLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRRL 618
           S EVDRERINHVCFS+DG+WLLFT+NL+GVT+EPVS PNQFQPYGDL+VVRLDGTGL RL
Sbjct: 542 SSEVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSWPNQFQPYGDLFVVRLDGTGLSRL 601

Query: 619 TWNGYENGTPTWHYGSELALSGLSLKDDVVGEKLEGKFEEPLWITF 662
           TWNGYENGTPTW+YGSE+ALSGLSLKD+VVGEKL+G F+EPLWITF
Sbjct: 602 TWNGYENGTPTWYYGSEVALSGLSLKDEVVGEKLKGNFDEPLWITF 646

BLAST of Sed0012789 vs. ExPASy TrEMBL
Match: A0A0A0K6G2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G419590 PE=4 SV=1)

HSP 1 Score: 1042.7 bits (2695), Expect = 6.4e-301
Identity = 499/646 (77.24%), Postives = 561/646 (86.84%), Query Frame = 0

Query: 19  NTPTGAVVFATVGRPQYGFDTFSVSLQSPTAEQRLTDGISVNFNAQFIDNQNSIVFISER 78
           + PTGAV+F TVG  QYGFD FSV L S T E++LTDGISVNFNAQF++NQ S+VFISER
Sbjct: 2   DNPTGAVLFTTVGLNQYGFDIFSVPLNSLTVERQLTDGISVNFNAQFLNNQLSVVFISER 61

Query: 79  SGSPKIYRSDSPNSAPELLPSATGSFFHDRPIIRNGRLFFTSAHENPHKSFASWSALYSS 138
           SGS +IY SDSPNS+P+LL SA GS FHDRPI+ NGRL F SAHENPHK F SW+ALYS+
Sbjct: 62  SGSSRIYLSDSPNSSPKLLASAPGSCFHDRPIVTNGRLLFISAHENPHKPFTSWAALYST 121

Query: 139 VLDGSDPVTRLTPPGFVDFSPAVSESGKFVAVASYGSRPWAGDFNELATEIVVFEADNPT 198
            LDG D VTRLTP G VDFSPAVSESGKFVAVASYGSR W G+F+EL  EIVVF++ +P 
Sbjct: 122 PLDGHDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNLEIVVFKSSDPG 181

Query: 199 RRVVVSGHGGWPSWCGDSAVYFHRTADDGWWSIFRVEIPENLE---SSVSSVPVRVTPAG 258
           +RVVV+G GGWPSW GDS V+FHR ADDGWWSIF+VEIPENL+   SSVS V +RVTPAG
Sbjct: 182 QRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLDSSRSSVSPVAIRVTPAG 241

Query: 259 LHCFTPAAMNDGKRVVVATRRPDDKFRHIEIFDSGSDEFDPITKKLNPGFHHYNPFVSPD 318
           LHCFTPAAMNDG+RVVVATRR D K+RHIEIFDS  +EF PIT+KLNP FHHYNPFVSPD
Sbjct: 242 LHCFTPAAMNDGRRVVVATRRADSKYRHIEIFDSELEEFIPITQKLNPEFHHYNPFVSPD 301

Query: 319 SKSVGYHRFRGESTQGELTVPHLDSVISPINNLRMIRINGFFPTPSPDNDFIAYNPDFVG 378
           S  +GYHRFRGESTQ EL +P+L  VISPI  L++IR+NG FPTPSPD D IA+NP F+G
Sbjct: 302 SNFIGYHRFRGESTQSEL-IPYLYPVISPIKELQIIRVNGSFPTPSPDGDLIAFNPGFIG 361

Query: 379 LKIVKSDSSKSWTVLKDRTAFYNSWSPTEKNVIFTSLGPIFGPARATVQIARITINYEDL 438
           L+IVK D SK  TVLKDRTAF NSWSPTEKNVI+TSLGPIFG   ATVQIARITI     
Sbjct: 362 LQIVKFDGSKCRTVLKDRTAFCNSWSPTEKNVIYTSLGPIFGAVTATVQIARITI----- 421

Query: 439 NGGDEDEIDHEVKILTKEDTGNNAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDGD 498
           N GD DE+ +EVKILTK++TGNNAFPACSPDG+FLVFRSGR+GHKNLYIVDA+ GE +G+
Sbjct: 422 NSGDSDEVSNEVKILTKDNTGNNAFPACSPDGKFLVFRSGRTGHKNLYIVDAMKGEFEGE 481

Query: 499 VRRLTDGPWIDTMPSWSPTGEFIAFSSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPEG 558
           +R+LTDGPWIDTMP+WSP G+ I FSSNMHNPKNTEAFSIYVI+PDGSGLRRV+VAGPEG
Sbjct: 482 LRQLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYVAGPEG 541

Query: 559 SDEVDRERINHVCFSKDGEWLLFTSNLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRRL 618
           S EVDRERINHVCFS+DG WLLFT+NL+GVT+EPVSLPNQFQPYGDL+VVRLDGTGLRRL
Sbjct: 542 SSEVDRERINHVCFSRDGNWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGTGLRRL 601

Query: 619 TWNGYENGTPTWHYGSELALSGLSLKDDVVGEKLEGKFEEPLWITF 662
           T N YENGTPTW+YGSELALSGLSLKD+VVGEKL+G F+EPLWI F
Sbjct: 602 TCNAYENGTPTWYYGSELALSGLSLKDEVVGEKLKGDFDEPLWIKF 641

BLAST of Sed0012789 vs. TAIR 10
Match: AT4G01870.1 (tolB protein-related )

HSP 1 Score: 879.8 bits (2272), Expect = 1.4e-255
Identity = 411/650 (63.23%), Postives = 513/650 (78.92%), Query Frame = 0

Query: 20  TPTGAVVFATVGRPQYGFDTFSVSLQSPTAEQRLTDGISVNFNAQFI-DNQNSIVFISER 79
           TP G ++F TVGR  YGFD FS+++ + + E+RLTDG+SVNFNAQF+ D  + +VF+SER
Sbjct: 3   TPKGTIIFTTVGRTHYGFDVFSLNI-ATSVERRLTDGVSVNFNAQFVNDKSDDVVFVSER 62

Query: 80  SGSPKIYRSDSPNSAPELLPSATGSFFHDRPII-RNGRLFFTSAHENPHKSFASWSALYS 139
           +GS +IY++ S  S PE +P A  S+FHDRPII +N RL+F SAHE P + F +WSALY+
Sbjct: 63  NGSARIYKTRSGISKPEQIPGAPESYFHDRPIITQNNRLYFISAHEQPDRYFKNWSALYT 122

Query: 140 SVLDGSD-PVTRLTPPGFVDFSPAVSESGKFVAVASYGSRPWAGDFNELATEIVVFEADN 199
             L+ +   VTR+TPP   DFSPAVS+SG F+AVASYG+R W G+F+E+ T+I VF+A  
Sbjct: 123 VELNSAKREVTRVTPPDTADFSPAVSQSGDFLAVASYGTRSWGGEFHEINTDITVFKASK 182

Query: 200 PTRRVVVSGHGGWPSWCGDSAVYFHRTADDGWWSIFRVEIPENLE--SSVSSVPVRVTPA 259
           P  RVV+   GGWP+W GDS V+FH  ADDGWWSIFRV+IPEN    +     P+RVTP+
Sbjct: 183 PETRVVICERGGWPTWSGDSTVFFHHQADDGWWSIFRVDIPENFTEYTDFPITPIRVTPS 242

Query: 260 GLHCFTPAAMNDGKRVVVATRRPDDKFRHIEIFDSGSDEFDPITKKLNPGFHHYNPFVSP 319
           GLHCFTPAA  DGKR+ +ATRR     RHIEI+D  +  F P+T+ LNP FHHYNPFVSP
Sbjct: 243 GLHCFTPAAFRDGKRIALATRRRGVNHRHIEIYDLENTTFQPVTESLNPSFHHYNPFVSP 302

Query: 320 DSKSVGYHRFRGESTQGELTVPHLDSVISPINNLRMIRINGFFPTPSPDNDFIAYNPDF- 379
           DS+ +GYHRFRGESTQGE  VP+++S++SPI  LR++RING FP+ SP+ D IA N DF 
Sbjct: 303 DSEFLGYHRFRGESTQGESIVPNIESIVSPIKTLRLLRINGSFPSSSPNGDLIALNSDFD 362

Query: 380 --VGLKIVKSDSSKSWTVLKDRTAFYNSWSPTEKNVIFTSLGPIFGPARATVQIARITIN 439
              G+K+ KSD SK WT++KDRTAFYNSWSPTE++VI+TSLGPIF PAR  VQIARI  +
Sbjct: 363 INGGIKVSKSDGSKRWTLIKDRTAFYNSWSPTERHVIYTSLGPIFSPARIAVQIARIKFD 422

Query: 440 YEDLNGGDEDEIDHEVKILTKEDTGNNAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGE 499
             DL   D++++  +VKILT E+TGNNAFP+CSPDG+ +VFRSGRSGHKNLYIVDAVNGE
Sbjct: 423 PSDLT-ADKEDLPCDVKILTLENTGNNAFPSCSPDGKSIVFRSGRSGHKNLYIVDAVNGE 482

Query: 500 -HDGDVRRLTDGPWIDTMPSWSPTGEFIAFSSNMHNPKNTEAFSIYVIKPDGSGLRRVHV 559
            + G +RRLTDGPWIDTMP WSP G+ I FSSN HNP+NT  F  YV++PDG+GLRR+ +
Sbjct: 483 SNGGGIRRLTDGPWIDTMPCWSPKGDLIGFSSNRHNPENTAVFGAYVVRPDGTGLRRIQI 542

Query: 560 AGPEGSDEVDRERINHVCFSKDGEWLLFTSNLAGVTSEPVSLPNQFQPYGDLYVVRLDGT 619
           +GPEGS+E  RER+NHV F+KDG+WL+F +NL+GVT+EPV++PNQFQPYGDLYVV+LDGT
Sbjct: 543 SGPEGSEEAARERVNHVSFNKDGDWLVFAANLSGVTAEPVTMPNQFQPYGDLYVVKLDGT 602

Query: 620 GLRRLTWNGYENGTPTWHYGSELALSGLSLKDDVVGEKLEGKFEEPLWIT 661
           GLRRLTWNGYE+GTPTWH   EL LS L+L     G+KLEG+FEEPLWI+
Sbjct: 603 GLRRLTWNGYEDGTPTWHTADELDLSQLNLNGQ-DGDKLEGQFEEPLWIS 649

BLAST of Sed0012789 vs. TAIR 10
Match: AT1G21680.1 (DPP6 N-terminal domain-like protein )

HSP 1 Score: 473.4 bits (1217), Expect = 3.0e-133
Identity = 270/673 (40.12%), Postives = 388/673 (57.65%), Query Frame = 0

Query: 9   ITLHFCTMD-KNTPTG---AVVFATVGRPQYGFDTFSVSLQSP---TAEQRLTDGISVNF 68
           + LH  T + +N+  G    ++F T+GR  Y FD F++S   P   + E R+TDG SVNF
Sbjct: 14  LRLHLSTAEHQNSNAGDGDTIIFTTLGRSHYEFDIFALSTTQPPSVSGELRITDGESVNF 73

Query: 69  NAQF----------------IDNQNS----IVFISERSGSPKIY-------------RSD 128
           N  F                I  ++S    +++++ER+G+  +Y             +  
Sbjct: 74  NGYFPSPSPALLSLLPDETLIQMEDSSPLHLIYVTERNGTSSLYYDLVYGGNSDFKTKRR 133

Query: 129 SPNSAP-----------ELLPSATGSFFHDRPIIRNGRLFFTSAHENPHKSFASWSALYS 188
           S   AP           + L   T + F D+P +    + + S HE+  +  ASW+A+YS
Sbjct: 134 SVLEAPSRVQVPLLSRFDHLSGMTVNSFKDKPSLSGEFIVYVSTHESSGEPRASWTAVYS 193

Query: 189 SVLDGSDPVTRLTPPGFVDFSPAVSESGKFVAVASYGSRPWAGDFNELATEIVVFEADNP 248
           + L  +    RLTP G  DFSPAVS SG   AVASYG R W G+  EL T+I VF   + 
Sbjct: 194 TELK-TGLTRRLTPSGVADFSPAVSPSGNLTAVASYGERGWTGEVEELRTDIYVFLTRDG 253

Query: 249 TRRVVVSGHGGWPSWCGDSAVYFH-RTADDGWWSIFRVEIPENLESSVSSVPV-RVTPAG 308
           + RV V  HGGWP W  +S +YFH R+ +DGW S++R  +PEN   +  SV + RVTP G
Sbjct: 254 SHRVKVVEHGGWPCWVDESTLYFHRRSEEDGWISVYRAILPENGPLTTESVTIQRVTPPG 313

Query: 309 LHCFTPA-AMNDGKRVVVATRRPDDKFRHIEIFDSGSDEFDPITKKLNPGFHHYNPFVSP 368
           +H FTPA + N+ + V VATRRP   +RH+E+FD   +EF  +T+ + P  HH NPF+SP
Sbjct: 314 VHAFTPATSPNNHEFVAVATRRPGSDYRHVELFDLKRNEFIELTRLVAPKSHHLNPFLSP 373

Query: 369 DSKSVGYHRFRGESTQGELTVPHLDSVISPINNLRMIRINGFFPTPSPDNDFIAYNPDFV 428
           DS  VGYH  RG++      +  L+++ +   +L + RI+G FP+ SP  D IAY     
Sbjct: 374 DSSRVGYHSCRGDANGRRSPLLFLENIQTTTRDLSLFRIDGSFPSFSPGGDRIAY-VKMP 433

Query: 429 GLKIVKSDSSKSWTVLKDRTAFYNSWSPTEKNVIFTSLGPIFGPARATVQIARITINYED 488
           G+ +VK D S    V K   AF  +W P    ++++S GP F   R  V +  I ++  D
Sbjct: 434 GVFVVKPDGSGQREVYKG-MAFSTAWDPVRPGIVYSSSGPTFATERTEVDVISIDVDAAD 493

Query: 489 LNGGDEDEIDHEVKILTKEDTGNNAFPACSPDGRFLVFRSGRSGHKNLYIVDAVNGEHDG 548
            +          V+ LT     NNAFP  SPDG+ +VFRSGR+GHKNLYI+DA  GE  G
Sbjct: 494 KSS--------SVRRLTTNGK-NNAFPWPSPDGKRIVFRSGRTGHKNLYIMDAEKGE-SG 553

Query: 549 DVRRLTDGPWIDTMPSWSPTGEFIAFSSNMHNPKNTEAFSIYVIKPDGSGLRRVHVAGPE 608
            + RLT+G W DTM +WSP GE+IAF+S+  +P  + +F +++I P+G+GLR++  +G  
Sbjct: 554 GLWRLTEGAWTDTMCNWSPDGEWIAFASDRESP-GSGSFELFLIHPNGTGLRKLIQSGTG 613

Query: 609 GSDEVDRERINHVCFSKDGEWLLFTSNLAGVTSEPVSLPNQFQPYGDLYVVRLDGTGLRR 628
           G       R NH  FS D + L+FTS+ AG+++EP+S P+ +QPYGD++ V+LDG+ +RR
Sbjct: 614 G-------RTNHPIFSPDSKSLVFTSDYAGISAEPISNPHHYQPYGDIFTVKLDGSNVRR 665

BLAST of Sed0012789 vs. TAIR 10
Match: AT1G21670.1 (LOCATED IN: cell wall, plant-type cell wall; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: WD40-like Beta Propeller (InterPro:IPR011659), Six-bladed beta-propeller, TolB-like (InterPro:IPR011042); BEST Arabidopsis thaliana protein match is: DPP6 N-terminal domain-like protein (TAIR:AT1G21680.1); Has 8461 Blast hits to 5060 proteins in 1257 species: Archae - 79; Bacteria - 5567; Metazoa - 37; Fungi - 70; Plants - 117; Viruses - 0; Other Eukaryotes - 2591 (source: NCBI BLink). )

HSP 1 Score: 436.8 bits (1122), Expect = 3.1e-122
Identity = 251/642 (39.10%), Postives = 360/642 (56.07%), Query Frame = 0

Query: 25  VVFATVGRPQYGFDTFSVSLQ----SPTAEQRLTDGISVNFNAQFIDNQNS--------- 84
           ++F T+GRP + FD F++       SP  E RLTDG S+NFN  F     +         
Sbjct: 34  ILFTTIGRPTFEFDIFTLPTSHRPPSPADEHRLTDGKSINFNGYFASPSTALISLLPKRT 93

Query: 85  --------IVFISERSGSPK----IYRSDSPNSAPELLPSATG---------SFFHDRPI 144
                   +++++ER+G+P     +  SD+  S  + +P  +G         +   D P+
Sbjct: 94  QIQPQDVHLIYVTERAGTPSLNYDVVHSDNVGSRIQ-VPLFSGEEQQSGMNVNSMKDTPV 153

Query: 145 IRNGRLFFTSAHENPHKSFASWSALYSSVLDGSDPVTRLTPPGFVDFSPAVSESGKFVAV 204
           + NG L   S HENP K  ASW+A+YS+ L  +    RLTP G  DFSPAVS SGK+ AV
Sbjct: 154 LTNGYLVHVSTHENPGKPMASWAAVYSTEL-RTKSTRRLTPLGIADFSPAVSPSGKWTAV 213

Query: 205 ASYGSRPWAGDF--NELATEIVVFEADNPTRRVVVSGHGGWPSWCGDSAVYFHRTADDGW 264
           AS+G + W       E+++++ VF   + T+RV V   GGWP W  DS +YFHR +DDGW
Sbjct: 214 ASFGEKGWTWSMVEKEISSDVYVFLTQDGTQRVKVVEQGGWPRWVDDSTLYFHRKSDDGW 273

Query: 265 WSIFRVEIPENLESSVSSVPV-RVTPAGLHCFTPA-AMNDGKRVVVATRRPDDKFRHIEI 324
            S++R  +P+    +  SV + RVTP GLH FTPA + N+   + VATRRP  + RH+E+
Sbjct: 274 ISVYRAILPKTGPVTTKSVTIQRVTPPGLHAFTPATSPNNNNFIAVATRRPGSEIRHVEL 333

Query: 325 FDSGSDEFDPITKKLNPGFHHYNPFVSPDSKSVGYHRFRGESTQGELTVPHLDSVISPIN 384
           FD   +EF  +T+ ++P  HH+NPF+SPDS  VGYH  RG++T  +     L S+ +  N
Sbjct: 334 FDLKKNEFVELTRLVSPKSHHFNPFLSPDSSRVGYHSCRGDATGRKTPRNLLQSLKTTSN 393

Query: 385 NLRMIRINGFFPTPSPDNDFIAYNPDFVGLKIVKSDSSKSWTVLKDRTAFYNSWSPTEKN 444
           +L + R +G FP+ SP+ D  A+   F G+ +V  D S     L  +  F   W P    
Sbjct: 394 DLSLFRFDGAFPSISPEGDRFAF-VSFTGVFVVNPDGS-GLRQLLPQMGFGTVWDPIRHG 453

Query: 445 VIFTSLGPIFGPARATVQIARITINYEDLNGGDEDEIDHEVKILTKEDTG-NNAFPACSP 504
           +++TS GP   P ++ + I  I +        D       VK LT   TG NNAFP  SP
Sbjct: 454 IVYTSSGPALAPGKSQIDILAINV--------DAPSPATAVKKLT--TTGENNAFPWPSP 513

Query: 505 DGRFLVFRSGRSGHKNLYIVDAVNGEHDGDVRRLTDGPWIDTMPSWSPTGEFIAFSSNMH 564
           DG+ +VFRS RSG KNLYI+DA  GE  G + RLT+G W DT+ +WSP G +I F+SN  
Sbjct: 514 DGKRIVFRSARSGTKNLYIMDAEKGE-SGGLFRLTNGNWNDTIATWSPDGNWIVFASNRE 573

Query: 565 NPKNTEAFSIYVIKPDGSGLRRVHVAGPEGSDEVDRERINHVCFSKDGEWLLFTSNLAGV 624
            P  T   +IYV+ PDG+GLR++       +  +      H  FS D + ++FT+  AG+
Sbjct: 574 FP-GTLLMNIYVVHPDGTGLRKL-------AQNLTGLVSMHPMFSPDSKRIVFTTIYAGI 633

Query: 625 TSEPVSLPNQFQPYGDLYVVRLDGTGLRRLTWNGYENGTPTW 628
           ++E +  P+   P  +++ V LDG+GL RLT N  E+G P W
Sbjct: 634 SAEQIGNPHFNVPSSEIFTVNLDGSGLTRLTHNSVEDGPPMW 652

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022954120.10.0e+0082.22uncharacterized protein LOC111456480 [Cucurbita moschata][more]
KAG6575727.10.0e+0082.06hypothetical protein SDJN03_26366, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023547812.10.0e+0082.22uncharacterized protein LOC111806666 [Cucurbita pepo subsp. pepo][more]
KAG7014283.10.0e+0081.75tolB, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022991715.10.0e+0080.97uncharacterized protein LOC111488250 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q3APB55.5e-1526.90Protein TolB homolog OS=Chlorobium chlorochromatii (strain CaD3) OX=340177 GN=to... [more]
Q3A0971.6e-1426.35Tol-Pal system protein TolB OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 1... [more]
Q0AC406.7e-1326.37Tol-Pal system protein TolB OS=Alkalilimnicola ehrlichii (strain ATCC BAA-1101 /... [more]
A8LHQ61.1e-1232.34Tol-Pal system protein TolB OS=Dinoroseobacter shibae (strain DSM 16493 / NCIMB ... [more]
Q9A3H46.3e-1131.74Tol-Pal system protein TolB OS=Caulobacter vibrioides (strain ATCC 19089 / CB15)... [more]
Match NameE-valueIdentityDescription
A0A6J1GRK10.0e+0082.22uncharacterized protein LOC111456480 OS=Cucurbita moschata OX=3662 GN=LOC1114564... [more]
A0A6J1JTR30.0e+0080.97uncharacterized protein LOC111488250 OS=Cucurbita maxima OX=3661 GN=LOC111488250... [more]
A0A5A7URS24.9e-31078.64TolB protein-related isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3CEJ87.1e-30878.17uncharacterized protein LOC103499982 OS=Cucumis melo OX=3656 GN=LOC103499982 PE=... [more]
A0A0A0K6G26.4e-30177.24Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G419590 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G01870.11.4e-25563.23tolB protein-related [more]
AT1G21680.13.0e-13340.12DPP6 N-terminal domain-like protein [more]
AT1G21670.13.1e-12239.10LOCATED IN: cell wall, plant-type cell wall; EXPRESSED IN: 23 plant structures; ... [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011042Six-bladed beta-propeller, TolB-likeGENE3D2.120.10.30coord: 560..636
e-value: 5.8E-7
score: 31.1
coord: 112..344
e-value: 3.4E-14
score: 54.8
IPR011042Six-bladed beta-propeller, TolB-likeGENE3D2.120.10.30coord: 349..559
e-value: 5.3E-29
score: 103.3
IPR011659WD40-like Beta PropellerPFAMPF07676PD40coord: 497..523
e-value: 7.3E-5
score: 22.6
coord: 455..484
e-value: 6.1E-4
score: 19.7
NoneNo IPR availablePANTHERPTHR32161DPP6 N-TERMINAL DOMAIN-LIKE PROTEINcoord: 20..661
NoneNo IPR availablePANTHERPTHR32161:SF9TOLB PROTEIN-LIKE PROTEINcoord: 20..661
NoneNo IPR availableSUPERFAMILY82171DPP6 N-terminal domain-likecoord: 358..633
NoneNo IPR availableSUPERFAMILY69304Tricorn protease N-terminal domaincoord: 41..290

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0012789.1Sed0012789.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis