Sed0012565 (gene) Chayote v1

Overview
NameSed0012565
Typegene
OrganismSechium edule (Chayote v1)
Description4-coumarate--CoA ligase-like 1
LocationLG04: 2140265 .. 2142786 (+)
RNA-Seq ExpressionSed0012565
SyntenySed0012565
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACAACAACCAAGCAATGGCTACCTGCATTCGAGATTTGGTAGAAGACGAGGAACATATTTTCCGCAGCCAACTTCCTGAGGTCCAAGTGCCTGATGATATCACATTGCCCGATTTCGTACTCCAGAATGCTGAATCCTATGCTGGTAATGTGGCGTTCGTGGAAGCCGTGAGCGGTAAGGCGTATACTTACCGTGAAGTAGTGAGAGACACGAATAGATTCGCTAAGGCCTTGAACTCTCTGGGGTTGAGGAAGGGCCAGGTGGTTATTGTTGTTCTACCTAATGTTGCAGAATATGCCATTGTTGCTTTAGGTATTATGGCTACTGGAGGTGTGTTTTCTGGTGTGAATCCAGCTGCTCACATATCAGAAATCAAAAAGCAGGTGGAGGCAGCAGACGCCAAACTCGTTGTCACGAACAGCGCAAACTTCGAAAAGGTAAAATGTAAACTGTTGGTCGTTTTCAGCCGTGATTATTTAGACCCTATTTGATAACCATTTGAATTTTTGTTTTTAGTTATTGAAACTTAAGCCTACAGGCTACAACTACTATTCCAACTTGTGATTGTCAATATTTTGGTATCTGCATTTTACTTGTATTTTTAAAAATGAAAGTTAAAAAAAATAGCTTTCGAATACTTGTTTTATTTATGGATTTTCGTTACAATTTATAGACATATTTTTTTTAAGAGAAGATGAATAAAAAACTAAATGGTTATCAAATTGGGTCTTAGAATTTCGACTTTGCTCACTTACTATCAATGCGGATCACACAGGTGAAGGAACTAAACCTACCGGTGATCGTGTTGGACGAGGAACTGATTGAAGGTTCCATGAACTGGTACAAACTGCTCGAAGCTGCGGATCGAGCAGGCAACAACTTTGTCAAAGAAGATATCAAGCAGACTGATTTATGTGCCCTTCCTTTCTCATCAGGCACCACAGGAGTTTCCAAAGGTGTAATGCTAACACACCGAAATCTAGTGGCTAACTTGTGTTCTACACTCTCCGGTGTGCCACAAGAAATCGAGGGCAAGGTCACGACTTTAGGCCTCATTCCGTTCTTCCACATTTACGGGATTACAGGCATATGTTGTGCCACACTTCGTAACAAGGGAAAAGTTGTTGTGATGGGAAGATTTGATCTCAGGACCTTCATCAATGCCCTGATAACACAGGAGGTCACATTTGCTCCAATTGTTCCTCCTATCATCTTGGCTTTGGTTAAGAATCCTATTGTGGAGGAATTCGATCTTAGCGGTCTCAAACTTCAAGCTATCATGACTGCAGCCGCTCCACTCGCTCCGGAACTTCAAACTGCCTTCGAGAAAAAGTTTCCCGGCGTTGATGTCCAAGAGGTAGTTAAGAGATTGATTCATTCCTCCAAATTATTAAACTACAACATGTAGCCCAACTTCCTGTTTTCTGCATATCGAAAACGAACCGAGCTCCAACTTCACATTGTTGAGCTAATCCACAGGGTTCTAACTCACATCTTCTTGCATATAACACTAACTAAATGCCATTGATTGCCGACAGGCATATGGACTAACCGAGCACAGTTGCATCACTCTCAACTATGGAAGTTTAGGCAAAGAGAATCTTGCTGCAAAGAAAAGCACAGTTGGACGCATTCTTCCTAATCTAGAAGTCAAATTCATCAACCCCGACAGCGGTCGGTCTCTGCCAAAGAATACTCCGGGCGAAATCTGTGTAAGAAGCCAGTGTGTGATGCAAGGTATAGAACATAAACCTAATCTTTCCTTGAGATCTCTAATAGATGCTAGCTAAAAAATTTCCCTTTTGTTTCAGGCTACTACAAAAATGAAGAGGAGACTTCGAGGACAATTGACAACAAAGGCTGGATGCACACAGGAGACATCGGATACATAGATGATGATGGCGATGTGTTTATCGTGGATCGCATTAAAGAGTTGATTAAGTACAAAGGCTTTCAAGTAAATACATAACATAACTTAGTTCAACTTTATCCATTGATTGATAAAAGAAAGCTCAAGATGTGAAAAATGATAGTTTGCAAATAGAGATATTTTTGTTTTGTAAATGTGAACAATGTAATGAATCTCTTTGATTTCTCTGCAGGTTGCTCCAGCTGAGTTAGAAGCAATTCTCCTTACTCATTCCTCCGTTGAAGATGCAGCTGTCGTGCCGTAAGATTCGCCTCACATTCATTTTGTTTATGTACTCATTACAATTCAAAGAGAAATTTTGAACAAATCAAATCCGAAATGGTTGAAACAACATTATGCTGCATCGGTATTTCAGGCTGCCAGATGAAGAAGCCGGGGAGATCCCAGCAGCAAGCGTCGTAATGGCTCGAAATGCAAACGAAACTGAAGATGAAATAATCAAATACGTCGCGTCAAACGTTGCACATTACAAGAAAGTCAGGCTCGTTCATTTTGTGGACACTATCCCGAAATCACCTTCGGGGAAAGTTATGAGGAGGTTGATCAAAGACAAGATGATCGAAAAGATTCGAGCCGAATCGGCCTCTTAA

mRNA sequence

AAACAACAACCAAGCAATGGCTACCTGCATTCGAGATTTGGTAGAAGACGAGGAACATATTTTCCGCAGCCAACTTCCTGAGGTCCAAGTGCCTGATGATATCACATTGCCCGATTTCGTACTCCAGAATGCTGAATCCTATGCTGGTAATGTGGCGTTCGTGGAAGCCGTGAGCGGTAAGGCGTATACTTACCGTGAAGTAGTGAGAGACACGAATAGATTCGCTAAGGCCTTGAACTCTCTGGGGTTGAGGAAGGGCCAGGTGGTTATTGTTGTTCTACCTAATGTTGCAGAATATGCCATTGTTGCTTTAGGTATTATGGCTACTGGAGGTGTGTTTTCTGGTGTGAATCCAGCTGCTCACATATCAGAAATCAAAAAGCAGGTGGAGGCAGCAGACGCCAAACTCGTTGTCACGAACAGCGCAAACTTCGAAAAGGTGAAGGAACTAAACCTACCGGTGATCGTGTTGGACGAGGAACTGATTGAAGGTTCCATGAACTGGTACAAACTGCTCGAAGCTGCGGATCGAGCAGGCAACAACTTTGTCAAAGAAGATATCAAGCAGACTGATTTATGTGCCCTTCCTTTCTCATCAGGCACCACAGGAGTTTCCAAAGGTGTAATGCTAACACACCGAAATCTAGTGGCTAACTTGTGTTCTACACTCTCCGGTGTGCCACAAGAAATCGAGGGCAAGGTCACGACTTTAGGCCTCATTCCGTTCTTCCACATTTACGGGATTACAGGCATATGTTGTGCCACACTTCGTAACAAGGGAAAAGTTGTTGTGATGGGAAGATTTGATCTCAGGACCTTCATCAATGCCCTGATAACACAGGAGGTCACATTTGCTCCAATTGTTCCTCCTATCATCTTGGCTTTGGTTAAGAATCCTATTGTGGAGGAATTCGATCTTAGCGGTCTCAAACTTCAAGCTATCATGACTGCAGCCGCTCCACTCGCTCCGGAACTTCAAACTGCCTTCGAGAAAAAGTTTCCCGGCGTTGATGTCCAAGAGGCATATGGACTAACCGAGCACAGTTGCATCACTCTCAACTATGGAAGTTTAGGCAAAGAGAATCTTGCTGCAAAGAAAAGCACAGTTGGACGCATTCTTCCTAATCTAGAAGTCAAATTCATCAACCCCGACAGCGGTCGGTCTCTGCCAAAGAATACTCCGGGCGAAATCTGTGTAAGAAGCCAGTGTGTGATGCAAGGCTACTACAAAAATGAAGAGGAGACTTCGAGGACAATTGACAACAAAGGCTGGATGCACACAGGAGACATCGGATACATAGATGATGATGGCGATGTGTTTATCGTGGATCGCATTAAAGAGTTGATTAAGTACAAAGGCTTTCAAGTTGCTCCAGCTGAGTTAGAAGCAATTCTCCTTACTCATTCCTCCGTTGAAGATGCAGCTGTCGTGCCGCTGCCAGATGAAGAAGCCGGGGAGATCCCAGCAGCAAGCGTCGTAATGGCTCGAAATGCAAACGAAACTGAAGATGAAATAATCAAATACGTCGCGTCAAACGTTGCACATTACAAGAAAGTCAGGCTCGTTCATTTTGTGGACACTATCCCGAAATCACCTTCGGGGAAAGTTATGAGGAGGTTGATCAAAGACAAGATGATCGAAAAGATTCGAGCCGAATCGGCCTCTTAA

Coding sequence (CDS)

ATGGCTACCTGCATTCGAGATTTGGTAGAAGACGAGGAACATATTTTCCGCAGCCAACTTCCTGAGGTCCAAGTGCCTGATGATATCACATTGCCCGATTTCGTACTCCAGAATGCTGAATCCTATGCTGGTAATGTGGCGTTCGTGGAAGCCGTGAGCGGTAAGGCGTATACTTACCGTGAAGTAGTGAGAGACACGAATAGATTCGCTAAGGCCTTGAACTCTCTGGGGTTGAGGAAGGGCCAGGTGGTTATTGTTGTTCTACCTAATGTTGCAGAATATGCCATTGTTGCTTTAGGTATTATGGCTACTGGAGGTGTGTTTTCTGGTGTGAATCCAGCTGCTCACATATCAGAAATCAAAAAGCAGGTGGAGGCAGCAGACGCCAAACTCGTTGTCACGAACAGCGCAAACTTCGAAAAGGTGAAGGAACTAAACCTACCGGTGATCGTGTTGGACGAGGAACTGATTGAAGGTTCCATGAACTGGTACAAACTGCTCGAAGCTGCGGATCGAGCAGGCAACAACTTTGTCAAAGAAGATATCAAGCAGACTGATTTATGTGCCCTTCCTTTCTCATCAGGCACCACAGGAGTTTCCAAAGGTGTAATGCTAACACACCGAAATCTAGTGGCTAACTTGTGTTCTACACTCTCCGGTGTGCCACAAGAAATCGAGGGCAAGGTCACGACTTTAGGCCTCATTCCGTTCTTCCACATTTACGGGATTACAGGCATATGTTGTGCCACACTTCGTAACAAGGGAAAAGTTGTTGTGATGGGAAGATTTGATCTCAGGACCTTCATCAATGCCCTGATAACACAGGAGGTCACATTTGCTCCAATTGTTCCTCCTATCATCTTGGCTTTGGTTAAGAATCCTATTGTGGAGGAATTCGATCTTAGCGGTCTCAAACTTCAAGCTATCATGACTGCAGCCGCTCCACTCGCTCCGGAACTTCAAACTGCCTTCGAGAAAAAGTTTCCCGGCGTTGATGTCCAAGAGGCATATGGACTAACCGAGCACAGTTGCATCACTCTCAACTATGGAAGTTTAGGCAAAGAGAATCTTGCTGCAAAGAAAAGCACAGTTGGACGCATTCTTCCTAATCTAGAAGTCAAATTCATCAACCCCGACAGCGGTCGGTCTCTGCCAAAGAATACTCCGGGCGAAATCTGTGTAAGAAGCCAGTGTGTGATGCAAGGCTACTACAAAAATGAAGAGGAGACTTCGAGGACAATTGACAACAAAGGCTGGATGCACACAGGAGACATCGGATACATAGATGATGATGGCGATGTGTTTATCGTGGATCGCATTAAAGAGTTGATTAAGTACAAAGGCTTTCAAGTTGCTCCAGCTGAGTTAGAAGCAATTCTCCTTACTCATTCCTCCGTTGAAGATGCAGCTGTCGTGCCGCTGCCAGATGAAGAAGCCGGGGAGATCCCAGCAGCAAGCGTCGTAATGGCTCGAAATGCAAACGAAACTGAAGATGAAATAATCAAATACGTCGCGTCAAACGTTGCACATTACAAGAAAGTCAGGCTCGTTCATTTTGTGGACACTATCCCGAAATCACCTTCGGGGAAAGTTATGAGGAGGTTGATCAAAGACAAGATGATCGAAAAGATTCGAGCCGAATCGGCCTCTTAA

Protein sequence

MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYREVVRDTNRFAKALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEIKKQVEAADAKLVVTNSANFEKVKELNLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEIEGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAKKSTVGRILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAGEIPAASVVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKMIEKIRAESAS
Homology
BLAST of Sed0012565 vs. NCBI nr
Match: XP_022945246.1 (4-coumarate--CoA ligase-like 1 [Cucurbita moschata])

HSP 1 Score: 1019.2 bits (2634), Expect = 1.3e-293
Identity = 517/550 (94.00%), Postives = 539/550 (98.00%), Query Frame = 0

Query: 1   MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYR 60
           MATCIRDLVEDEEHIFRSQLPEVQVPDDITLP+FVLQNAESYA NVAFVEAVSGKAYTYR
Sbjct: 57  MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYR 116

Query: 61  EVVRDTNRFAKALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEI 120
           EVVRDT+RFAKAL+SL L+KGQVVIVVLPNVAEYAIVALGIMA GGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 KKQVEAADAKLVVTNSANFEKVKELNLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKE 180
           KKQVE ADAKLVVTNS++FEKVKEL LPVIV+ EELIEGSMNW+KLLEAADRAGNNFVKE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEIEGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE+ GKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV+EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAK 360
           LSGLKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYGS+GK+NLAAK
Sbjct: 357 LSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAK 416

Query: 361 KSTVGRILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K+TVGRILPNLEVKFI+PDSGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID+KGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWM 476

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAG 480
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTH S+EDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 540
           EIPAAS+VMA NA ETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIK+KM
Sbjct: 537 EIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IEKIRAESAS 551
           IEKIRAE+AS
Sbjct: 597 IEKIRAEAAS 606

BLAST of Sed0012565 vs. NCBI nr
Match: XP_023541569.1 (4-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1015.0 bits (2623), Expect = 2.5e-292
Identity = 515/550 (93.64%), Postives = 537/550 (97.64%), Query Frame = 0

Query: 1   MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYR 60
           MATCIRD VEDEEHIFRSQLPEVQVPDDITLP+FVLQNAESYA NVAFVEAVSG AYTYR
Sbjct: 57  MATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGNAYTYR 116

Query: 61  EVVRDTNRFAKALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEI 120
           EVVRDT+RFAKAL+SL L+KGQVVIVVLPNVAEYAIVALGIMA GGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 KKQVEAADAKLVVTNSANFEKVKELNLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKE 180
           KKQVE ADAKLVVTNS++FEKVKEL LPVIV+ EELIEGSMNW+KLLEAADRAGNNFVKE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEIEGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE+ GKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV+EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAK 360
           LSGLKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYGS+GK+NLAAK
Sbjct: 357 LSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAK 416

Query: 361 KSTVGRILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K+TVGRILPNLEVKFI+PDSGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID+KGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWM 476

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAG 480
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTH S+EDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 540
           EIPAAS+VMA NA ETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIK+KM
Sbjct: 537 EIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IEKIRAESAS 551
           IEKIRAE+AS
Sbjct: 597 IEKIRAETAS 606

BLAST of Sed0012565 vs. NCBI nr
Match: KAG7012722.1 (4-coumarate--CoA ligase-like 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1013.8 bits (2620), Expect = 5.5e-292
Identity = 514/550 (93.45%), Postives = 537/550 (97.64%), Query Frame = 0

Query: 1   MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYR 60
           MATCIRD VEDEEHIFRSQLPEVQVPDDITLP+FVLQNAESYA NVAFVEAVSGKAYTYR
Sbjct: 57  MATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYR 116

Query: 61  EVVRDTNRFAKALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEI 120
           EVVRDT+RFAKAL+SL L+KGQVVIVVLPNVAEYAIVALGIMA GGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 KKQVEAADAKLVVTNSANFEKVKELNLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKE 180
           KKQVE ADAKLVVTNS++FEKVKEL LPVIV+ EELIEGSMNW++LLEAADRAGNNFVKE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEIEGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE+ GKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV+EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAK 360
           LSGLKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYGS+GK+NLAAK
Sbjct: 357 LSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAK 416

Query: 361 KSTVGRILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K+TVGRILPNLEVKFI+PDSGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID+KGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWM 476

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAG 480
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTH S+EDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 540
           EIPAAS+VM  NA ETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIK+KM
Sbjct: 537 EIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IEKIRAESAS 551
           IEKIRAE+AS
Sbjct: 597 IEKIRAETAS 606

BLAST of Sed0012565 vs. NCBI nr
Match: XP_038893074.1 (4-coumarate--CoA ligase-like 1 [Benincasa hispida])

HSP 1 Score: 1013.4 bits (2619), Expect = 7.2e-292
Identity = 515/550 (93.64%), Postives = 536/550 (97.45%), Query Frame = 0

Query: 1   MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYR 60
           MATC RDLVEDEEHIFRSQLPEVQVPDDITLP+FVLQNAESYA NVAFVEAVSGKAYTYR
Sbjct: 1   MATCNRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60

Query: 61  EVVRDTNRFAKALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEI 120
           EVVRDTNRF+KAL SL L+KGQVVIVVLPNVAEYAIVALGIMA GGVFSGVNPAAHISEI
Sbjct: 61  EVVRDTNRFSKALWSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120

Query: 121 KKQVEAADAKLVVTNSANFEKVKELNLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKE 180
           KKQVE A+AKLV+TNSANFEKVKEL LPVI+L EELIEGSMNW+KLLEAADRAGNN VKE
Sbjct: 121 KKQVEVAEAKLVITNSANFEKVKELKLPVILLGEELIEGSMNWHKLLEAADRAGNNCVKE 180

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEIEGKVTTLGLIPFFHI 240
           DIKQ DLCALPFSSGTTGVSKGVMLTHRNL+ANLCSTLSGVPQE+EGKVTTLGLIPFFHI
Sbjct: 181 DIKQNDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPQEMEGKVTTLGLIPFFHI 240

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD
Sbjct: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAK 360
           LSGLKLQAIMTAAAPLAPELQ+AFE+KFPGVDVQEAYGLTEHSCITLNYGS+GKENLAAK
Sbjct: 301 LSGLKLQAIMTAAAPLAPELQSAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLAAK 360

Query: 361 KSTVGRILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K+TVGRILPNLEVKFI+PDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM
Sbjct: 361 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAG 480
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTH S+EDAAVVPLPDEEAG
Sbjct: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480

Query: 481 EIPAASVVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 540
           EIPAASVVMA N+ ETE+EIIK+VASNVA+YKKVRLVHFVD IPKSPSGKVMRRL+K+KM
Sbjct: 481 EIPAASVVMAPNSKETEEEIIKHVASNVANYKKVRLVHFVDAIPKSPSGKVMRRLVKEKM 540

Query: 541 IEKIRAESAS 551
           IEKIRAESAS
Sbjct: 541 IEKIRAESAS 550

BLAST of Sed0012565 vs. NCBI nr
Match: XP_022966799.1 (4-coumarate--CoA ligase-like 1 [Cucurbita maxima])

HSP 1 Score: 1010.7 bits (2612), Expect = 4.6e-291
Identity = 511/550 (92.91%), Postives = 535/550 (97.27%), Query Frame = 0

Query: 1   MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYR 60
           MATCIRD  EDEEHIFRSQLPEVQVPDDITLP+FVLQNAE YA NVAFVEAVSGKAYTYR
Sbjct: 57  MATCIRDSAEDEEHIFRSQLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSGKAYTYR 116

Query: 61  EVVRDTNRFAKALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEI 120
           EVVRDT+RFAKAL+SL L+KGQ+VIVVLPNVAEYAIVALGIMA GGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 KKQVEAADAKLVVTNSANFEKVKELNLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKE 180
           KKQVE ADAKLVVTNS++FEKVKEL LPVIV+ EELIEGSMNW+KLLEAADRAGNNFVKE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEIEGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSG+PQE+ GKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV+EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAK 360
           LS LKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYG++GK+NLAAK
Sbjct: 357 LSSLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGKDNLAAK 416

Query: 361 KSTVGRILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K+TVGRILPNLEVKFI+PDSGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTIDNKGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNKGWM 476

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAG 480
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTH S+EDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 540
           EIPAAS+VMA NA ETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIK+KM
Sbjct: 537 EIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IEKIRAESAS 551
           IEKIRAE+AS
Sbjct: 597 IEKIRAETAS 606

BLAST of Sed0012565 vs. ExPASy Swiss-Prot
Match: Q9LQ12 (4-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 3.2e-226
Identity = 392/532 (73.68%), Postives = 453/532 (85.15%), Query Frame = 0

Query: 10  EDEEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYREVVRDTNRF 69
           ED E+IFRS  P V +PD +TLP+FVLQ  E Y  NVAFVEAV+GKA TY +VVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 70  AKALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEIKKQVEAADA 129
           AKAL SLGLRKGQV++VVLPNVAEY I+ALGIM+ GGVFSG NP A +SEIKKQVEA+ A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 130 KLVVTNSANFEKVKELNLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKEDIKQTDLCA 189
           + ++T++ N+EKVK L LPVIVL EE IEG++NW  LLEA D+ G+    E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 190 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEIEGKVTTLGLIPFFHIYGITGICCA 249
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  E+ G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 250 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAI 309
           T++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 310 MTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAKKSTVGRILP 369
           MTAAAPLAPEL TAFE KFP V VQEAYGLTEHSCITL +G   K    AK+++VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 370 NLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYID 429
           NLEVKFI+PD+GRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 430 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAGEIPAASVVM 489
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTH SVED AVVPLPDEEAGEIPAA VV+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 490 ARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKMI 542
              A E E++I+ +VA+NVAHYKKVR VHFVD+IPKS SGK+MRRL++DK++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of Sed0012565 vs. ExPASy Swiss-Prot
Match: Q7XXL2 (4-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9 PE=2 SV=2)

HSP 1 Score: 708.8 bits (1828), Expect = 4.9e-203
Identity = 352/539 (65.31%), Postives = 436/539 (80.89%), Query Frame = 0

Query: 10  EDEEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVS-GKAYTYREVVRDTNR 69
           E++EH+FRS+ P V VPD +T+P+FVL  AE+YA  VA VEA + G++YTY EV RDT R
Sbjct: 14  EEQEHVFRSRFPPVAVPDGVTVPEFVLDGAEAYADRVALVEAAAGGRSYTYGEVARDTAR 73

Query: 70  FAKALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEIKKQVEAAD 129
           FA+AL S+G+RKG VV+V LPN+A Y +V+LGIM+ G VFSGVNP A  +EIKKQVE ++
Sbjct: 74  FARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSE 133

Query: 130 AKLVVTNSANFEKVKELNLPVI-VLDEELIEGSMNWYKLLEAADRAGNNFVKED-IKQTD 189
           AKLVV N   F+KVK+  +PVI V D E + G+++W  LL AADR G   V  D  +Q+D
Sbjct: 134 AKLVVANEVAFDKVKDAGVPVIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSD 193

Query: 190 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEIEGKVTTLGLIPFFHIYGITGI 249
           LCALP+SSGTTGVSKGVML+HRNLV+NLCS++  V  E  G+V TLGL+PFFHIYGITGI
Sbjct: 194 LCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAVAPETAGQVVTLGLMPFFHIYGITGI 253

Query: 250 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKL 309
           CCATLR+KG VVVM RFDLRTF+ AL+   V FAP+VPP++LA+VK+P+ +EFDLS L L
Sbjct: 254 CCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAPLVPPVMLAMVKSPVADEFDLSDLAL 313

Query: 310 QAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAKKSTVGR 369
           +++MTAAAPLAP+L  AF++KFPGV V+EAYGLTEHSCITL + +       AKKS+VG 
Sbjct: 314 KSVMTAAAPLAPDLLAAFQRKFPGVQVEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGF 373

Query: 370 ILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIG 429
           ILPNLEVKF++PD+GRSLP NTPGE+CVRSQ VMQGYYK +EET RT+D KGW+HTGD+G
Sbjct: 374 ILPNLEVKFVDPDTGRSLPANTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVG 433

Query: 430 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAGEIPAAS 489
           YID DGDVFIVDRIKELIKYKGFQVAPAELEA+LL+H SVEDAAV  +PDEEAGE+P A 
Sbjct: 434 YIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVAC 493

Query: 490 VVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKMIEKIR 546
           VV    A E E+EI+ YVA  VA YK+VR++H VD IPKS SGK++RR ++D+ I++++
Sbjct: 494 VVRRHGAEEGEEEIVAYVAERVASYKRVRVLHIVDAIPKSVSGKILRRQLRDEFIKRMK 552

BLAST of Sed0012565 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 1.3e-115
Identity = 212/535 (39.63%), Postives = 332/535 (62.06%), Query Frame = 0

Query: 12  EEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYREVVRDTNRFAK 71
           E+ IFRS+LP++ +P  + L  +  +N          +   +G+ +TY +V   + + A 
Sbjct: 9   EDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 68

Query: 72  ALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEIKKQVEAADAKL 131
            LN LG+++G  ++++LPN  EY    LG    G + +  NP    +E+ KQ++A+ AKL
Sbjct: 69  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKL 128

Query: 132 VVTNSANFEKVK----ELNLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKEDIKQTDL 191
           ++T +   +KVK    E N+ +I +D +  +  +++ KL+EA +      V   I   D+
Sbjct: 129 IITQACYVDKVKDYAAEKNIQIICID-DAPQDCLHFSKLMEADESEMPEVV---INSDDV 188

Query: 192 CALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEI--EGKVTTLGLIPFFHIYGITG 251
            ALP+SSGTTG+ KGVMLTH+ LV ++   + G    +    +   + ++P FHIY +  
Sbjct: 189 VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNA 248

Query: 252 ICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLK 311
           + C  LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V+++DLS ++
Sbjct: 249 VLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVR 308

Query: 312 LQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAKKSTVG 371
              +M+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE    K    G
Sbjct: 309 --TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACG 368

Query: 372 RILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDI 431
            ++ N E+K ++P++  SLP+N  GEIC+R   +M+GY  + E T  TID +GW+HTGDI
Sbjct: 369 TVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDI 428

Query: 432 GYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAGEIPAA 491
           G+IDDD ++FIVDR+KE+IKYKGFQVAPAELEA+LLTH ++ DAAVVP+ DE+AGE+P A
Sbjct: 429 GFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVA 488

Query: 492 SVVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 541
            VV       TE+EI ++V+  V  YK++  V FVD IPKSPSGK++R+ ++ ++
Sbjct: 489 FVVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARI 537

BLAST of Sed0012565 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 2.3e-115
Identity = 209/532 (39.29%), Postives = 332/532 (62.41%), Query Frame = 0

Query: 15  IFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYREVVRDTNRFAKALN 74
           IFRS+LP++ +P  + L  +  +N   ++     +   +   YTY +V   + + A  LN
Sbjct: 13  IFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVELTSRKVAAGLN 72

Query: 75  SLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEIKKQVEAADAKLVVT 134
            LG+++   ++++LPN  E+    +G    G + +  NP    +E+ KQ +A++AKL++T
Sbjct: 73  KLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASNAKLIIT 132

Query: 135 NSANFEKVKEL----NLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKEDIKQTDLCAL 194
            +    KVK+     NL VI +D    EG +++ +L +A +   ++     I+  D+ AL
Sbjct: 133 QACFVNKVKDYAFDNNLNVICID-SAPEGCIHFSELTQADE---HDIPDVKIQSDDVVAL 192

Query: 195 PFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEI--EGKVTTLGLIPFFHIYGITGICC 254
           P+SSGTTG+ KGVMLTH+ LV ++   + G    +    +   + ++P FHIY +  +  
Sbjct: 193 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNSVLL 252

Query: 255 ATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQA 314
             LR    +++M +FD+  F   +   +VT  P VPPI+LA+ K+P+V+ +DLS ++   
Sbjct: 253 CGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDLSSVR--T 312

Query: 315 IMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAKKSTVGRIL 374
           +M+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE    K    G ++
Sbjct: 313 VMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVV 372

Query: 375 PNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYI 434
            N E+K ++PD+G SLP+N PGEIC+R   +M+GY  +   T+RTID +GW+HTGDIGYI
Sbjct: 373 RNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHTGDIGYI 432

Query: 435 DDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAGEIPAASVV 494
           D+D ++FIVDR+KELIKYKGFQVAPAELEA+LL H ++ DAAVVP+ DE+AGE+P A VV
Sbjct: 433 DNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVV 492

Query: 495 MARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 541
            +  ++ TEDE+  +V+  V  YK+++ V FV+T+PKSPSGK++R+ ++ ++
Sbjct: 493 RSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARL 538

BLAST of Sed0012565 vs. ExPASy Swiss-Prot
Match: P14913 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 2.3e-115
Identity = 213/535 (39.81%), Postives = 332/535 (62.06%), Query Frame = 0

Query: 12  EEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYREVVRDTNRFAK 71
           E+ IFRS+LP++ +P  + L  +  +N          +   +G+ +TY +V   + + A 
Sbjct: 9   EDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 68

Query: 72  ALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEIKKQVEAADAKL 131
            LN LG+++G  ++++LPN  EY    LG    G + +  NP    +E+ KQ++A+ AKL
Sbjct: 69  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAKL 128

Query: 132 VVTNSANFEKVK----ELNLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKEDIKQTDL 191
           ++T +   +KVK    E N+ +I +D +  +  +++ KL+EA +      V   I   D+
Sbjct: 129 IITQACYVDKVKDYAAEKNIQIICID-DAPQDCLHFSKLMEADESEMPEVV---IDSDDV 188

Query: 192 CALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEI--EGKVTTLGLIPFFHIYGITG 251
            ALP+SSGTTG+ KGVMLTH+ LV ++   + G    +    +   + ++P FHIY +  
Sbjct: 189 VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNA 248

Query: 252 ICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLK 311
           + C  LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V+++DLS ++
Sbjct: 249 VLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVR 308

Query: 312 LQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAKKSTVG 371
              +M+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE    K    G
Sbjct: 309 --TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACG 368

Query: 372 RILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDI 431
            ++ N E+K ++P++  SLP+N  GEIC+R   +M+GY  + E T  TID +GW+HTGDI
Sbjct: 369 TVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDI 428

Query: 432 GYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAGEIPAA 491
           G+IDDD ++FIVDR+KE+IKYKGFQVAPAELEA+LLTH ++ DAAVVP+ DE+AGE+P A
Sbjct: 429 GFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVA 488

Query: 492 SVVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 541
            VV       TE+EI ++V+  V  YK++  V FVD IPKSPSGK++R+ ++ K+
Sbjct: 489 FVVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKI 537

BLAST of Sed0012565 vs. ExPASy TrEMBL
Match: A0A6J1G0B4 (4-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=4 SV=1)

HSP 1 Score: 1019.2 bits (2634), Expect = 6.3e-294
Identity = 517/550 (94.00%), Postives = 539/550 (98.00%), Query Frame = 0

Query: 1   MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYR 60
           MATCIRDLVEDEEHIFRSQLPEVQVPDDITLP+FVLQNAESYA NVAFVEAVSGKAYTYR
Sbjct: 57  MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYR 116

Query: 61  EVVRDTNRFAKALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEI 120
           EVVRDT+RFAKAL+SL L+KGQVVIVVLPNVAEYAIVALGIMA GGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 KKQVEAADAKLVVTNSANFEKVKELNLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKE 180
           KKQVE ADAKLVVTNS++FEKVKEL LPVIV+ EELIEGSMNW+KLLEAADRAGNNFVKE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEIEGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE+ GKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV+EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAK 360
           LSGLKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYGS+GK+NLAAK
Sbjct: 357 LSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAK 416

Query: 361 KSTVGRILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K+TVGRILPNLEVKFI+PDSGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID+KGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWM 476

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAG 480
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTH S+EDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 540
           EIPAAS+VMA NA ETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIK+KM
Sbjct: 537 EIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IEKIRAESAS 551
           IEKIRAE+AS
Sbjct: 597 IEKIRAEAAS 606

BLAST of Sed0012565 vs. ExPASy TrEMBL
Match: A0A6J1HUV5 (4-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 SV=1)

HSP 1 Score: 1010.7 bits (2612), Expect = 2.2e-291
Identity = 511/550 (92.91%), Postives = 535/550 (97.27%), Query Frame = 0

Query: 1   MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYR 60
           MATCIRD  EDEEHIFRSQLPEVQVPDDITLP+FVLQNAE YA NVAFVEAVSGKAYTYR
Sbjct: 57  MATCIRDSAEDEEHIFRSQLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSGKAYTYR 116

Query: 61  EVVRDTNRFAKALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEI 120
           EVVRDT+RFAKAL+SL L+KGQ+VIVVLPNVAEYAIVALGIMA GGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 KKQVEAADAKLVVTNSANFEKVKELNLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKE 180
           KKQVE ADAKLVVTNS++FEKVKEL LPVIV+ EELIEGSMNW+KLLEAADRAGNNFVKE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEIEGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSG+PQE+ GKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV+EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAK 360
           LS LKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYG++GK+NLAAK
Sbjct: 357 LSSLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGKDNLAAK 416

Query: 361 KSTVGRILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K+TVGRILPNLEVKFI+PDSGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTIDNKGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNKGWM 476

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAG 480
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTH S+EDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 540
           EIPAAS+VMA NA ETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIK+KM
Sbjct: 537 EIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IEKIRAESAS 551
           IEKIRAE+AS
Sbjct: 597 IEKIRAETAS 606

BLAST of Sed0012565 vs. ExPASy TrEMBL
Match: A0A6J1DG05 (4-coumarate--CoA ligase-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 5.2e-288
Identity = 508/550 (92.36%), Postives = 533/550 (96.91%), Query Frame = 0

Query: 1   MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYR 60
           M T IRDLVEDEEHIFRSQLPEVQVPDDITLP+FVLQNAESYA NVAFVEAVSGKAYTYR
Sbjct: 60  MGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 119

Query: 61  EVVRDTNRFAKALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEI 120
           EVVRDT RFAKAL+SL LRKGQVVIVVLPNVAEYAIVALGIMA GGVFSGVNPAAHISEI
Sbjct: 120 EVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 179

Query: 121 KKQVEAADAKLVVTNSANFEKVKELNLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKE 180
           KKQVEAADAKLVVTNS NFEKVKEL LPVIVL EELIEGSMNW+KLLEAADRAGNN VKE
Sbjct: 180 KKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNNHVKE 239

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEIEGKVTTLGLIPFFHI 240
           DIKQ+DLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVP+E EGKVTTLGLIPFFHI
Sbjct: 240 DIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTTLGLIPFFHI 299

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD
Sbjct: 300 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 359

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAK 360
           LS LKLQAIMTAAAPLAPE+QTAFEKKFPGVDVQEAYGLTEHSCITLNYGS+GKENL AK
Sbjct: 360 LSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 419

Query: 361 KSTVGRILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K+TVGRILPNLEVKFI+P+SGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTIDN+GW+
Sbjct: 420 KNTVGRILPNLEVKFIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNRGWL 479

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAG 480
           HTGDIGYIDD+GDVFIVDRIKELIKYKGFQVAPAELEAILLTH S+EDAAVVPLPDEEAG
Sbjct: 480 HTGDIGYIDDNGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 539

Query: 481 EIPAASVVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 540
           EIPAASVV+A N+ ETE+EII+YVASNVA+YKKVR+VHFVD+IPKSPSGKVMRRLIK+KM
Sbjct: 540 EIPAASVVIAPNSKETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKVMRRLIKEKM 599

Query: 541 IEKIRAESAS 551
           I+KIRA++AS
Sbjct: 600 IDKIRAKTAS 609

BLAST of Sed0012565 vs. ExPASy TrEMBL
Match: A0A6J1DEE1 (4-coumarate--CoA ligase-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 5.2e-288
Identity = 508/550 (92.36%), Postives = 533/550 (96.91%), Query Frame = 0

Query: 1   MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYR 60
           M T IRDLVEDEEHIFRSQLPEVQVPDDITLP+FVLQNAESYA NVAFVEAVSGKAYTYR
Sbjct: 9   MGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 68

Query: 61  EVVRDTNRFAKALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEI 120
           EVVRDT RFAKAL+SL LRKGQVVIVVLPNVAEYAIVALGIMA GGVFSGVNPAAHISEI
Sbjct: 69  EVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 128

Query: 121 KKQVEAADAKLVVTNSANFEKVKELNLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKE 180
           KKQVEAADAKLVVTNS NFEKVKEL LPVIVL EELIEGSMNW+KLLEAADRAGNN VKE
Sbjct: 129 KKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNNHVKE 188

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEIEGKVTTLGLIPFFHI 240
           DIKQ+DLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVP+E EGKVTTLGLIPFFHI
Sbjct: 189 DIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTTLGLIPFFHI 248

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD
Sbjct: 249 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 308

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAK 360
           LS LKLQAIMTAAAPLAPE+QTAFEKKFPGVDVQEAYGLTEHSCITLNYGS+GKENL AK
Sbjct: 309 LSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 368

Query: 361 KSTVGRILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K+TVGRILPNLEVKFI+P+SGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTIDN+GW+
Sbjct: 369 KNTVGRILPNLEVKFIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNRGWL 428

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAG 480
           HTGDIGYIDD+GDVFIVDRIKELIKYKGFQVAPAELEAILLTH S+EDAAVVPLPDEEAG
Sbjct: 429 HTGDIGYIDDNGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 488

Query: 481 EIPAASVVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 540
           EIPAASVV+A N+ ETE+EII+YVASNVA+YKKVR+VHFVD+IPKSPSGKVMRRLIK+KM
Sbjct: 489 EIPAASVVIAPNSKETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKVMRRLIKEKM 548

Query: 541 IEKIRAESAS 551
           I+KIRA++AS
Sbjct: 549 IDKIRAKTAS 558

BLAST of Sed0012565 vs. ExPASy TrEMBL
Match: A0A0A0KRZ5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589320 PE=4 SV=1)

HSP 1 Score: 985.3 bits (2546), Expect = 1.0e-283
Identity = 495/550 (90.00%), Postives = 531/550 (96.55%), Query Frame = 0

Query: 1   MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYR 60
           MATC +D V+DEEHIF SQLPEVQVP DITLP+FVLQNAESYA NVAFVEA+SGKAYTYR
Sbjct: 1   MATCNQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYR 60

Query: 61  EVVRDTNRFAKALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEI 120
           EV+RDTNRF+KAL SL L+KG VVIVVLPNVAEYAIVALGIMA GGVFSGVNPAAHISEI
Sbjct: 61  EVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120

Query: 121 KKQVEAADAKLVVTNSANFEKVKELNLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKE 180
           KKQVE A+AKLVVTN+ANFEKV+EL LPVI+L+EEL+EG+MNW+KLLEAADRAGNNFVKE
Sbjct: 121 KKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE 180

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEIEGKVTTLGLIPFFHI 240
           +IKQTDLCALPFSSGTTGVSKGVMLTHRNLVAN+CSTLSGVPQE+EGKVTTLGLIPFFHI
Sbjct: 181 EIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHI 240

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICC+TLRNKGKVVVMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIVEEFD
Sbjct: 241 YGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFD 300

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAK 360
           LS LKLQAIMTAAAPLAPELQTAFE+KFPGVDVQEAYGLTEH CITLNYGS+GKENL+AK
Sbjct: 301 LSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAK 360

Query: 361 KSTVGRILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K+TVGRILPNLEVKFI+PD+GRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTID KGWM
Sbjct: 361 KNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWM 420

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAG 480
           HTGDIGYIDDDG+VFIVDRIKELIKYKGFQVAPAELEAILLTH S+EDAAVVPLPDEEAG
Sbjct: 421 HTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480

Query: 481 EIPAASVVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 540
           EIPAASVVMA N+ ETE++IIK+VASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIK+KM
Sbjct: 481 EIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540

Query: 541 IEKIRAESAS 551
           IEKIRA+ ++
Sbjct: 541 IEKIRADESA 550

BLAST of Sed0012565 vs. TAIR 10
Match: AT1G62940.1 (acyl-CoA synthetase 5 )

HSP 1 Score: 785.8 bits (2028), Expect = 2.3e-227
Identity = 392/532 (73.68%), Postives = 453/532 (85.15%), Query Frame = 0

Query: 10  EDEEHIFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYREVVRDTNRF 69
           ED E+IFRS  P V +PD +TLP+FVLQ  E Y  NVAFVEAV+GKA TY +VVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 70  AKALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEIKKQVEAADA 129
           AKAL SLGLRKGQV++VVLPNVAEY I+ALGIM+ GGVFSG NP A +SEIKKQVEA+ A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 130 KLVVTNSANFEKVKELNLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKEDIKQTDLCA 189
           + ++T++ N+EKVK L LPVIVL EE IEG++NW  LLEA D+ G+    E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 190 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEIEGKVTTLGLIPFFHIYGITGICCA 249
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  E+ G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 250 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAI 309
           T++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 310 MTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAKKSTVGRILP 369
           MTAAAPLAPEL TAFE KFP V VQEAYGLTEHSCITL +G   K    AK+++VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 370 NLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYID 429
           NLEVKFI+PD+GRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 430 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAGEIPAASVVM 489
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTH SVED AVVPLPDEEAGEIPAA VV+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 490 ARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKMI 542
              A E E++I+ +VA+NVAHYKKVR VHFVD+IPKS SGK+MRRL++DK++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of Sed0012565 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 409.5 bits (1051), Expect = 4.4e-114
Identity = 204/531 (38.42%), Postives = 328/531 (61.77%), Query Frame = 0

Query: 15  IFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYREVVRDTNRFAKALN 74
           IFRS+LP++ +P+ + L D++ +N   +A     +   +G+ YTY +V   + + A  L+
Sbjct: 22  IFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAGLH 81

Query: 75  SLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEIKKQVEAADAKLVVT 134
           +LG+++  VV+++LPN  E  +  L     G + +  NP    +EI KQ +A+ AKL+VT
Sbjct: 82  NLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVT 141

Query: 135 NSANFEKVKEL---NLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKEDIKQTDLCALP 194
            S   +K+K L    + ++  D + I  +   +  L  ++    + + E I   D+ ALP
Sbjct: 142 QSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDVVALP 201

Query: 195 FSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEI--EGKVTTLGLIPFFHIYGITGICCA 254
           FSSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P FHIY +  I   
Sbjct: 202 FSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSIMLC 261

Query: 255 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAI 314
           +LR    +++M +F++   +  +   +VT A +VPPI+LA+ K+P  E++DLS +++  +
Sbjct: 262 SLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRM--V 321

Query: 315 MTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAKKSTVGRILP 374
            + AAPL  EL+ A   KFP   + + YG+TE   +        KE    K    G ++ 
Sbjct: 322 KSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVR 381

Query: 375 NLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYID 434
           N E+K ++PD+G SLP+N PGEIC+R   +M+GY  +   T+ TID  GW+HTGD+G+ID
Sbjct: 382 NAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFID 441

Query: 435 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAGEIPAASVVM 494
           DD ++FIVDR+KELIKYKGFQVAPAELE++L+ H  + D AVV + +E+AGE+P A VV 
Sbjct: 442 DDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVVR 501

Query: 495 ARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 541
           ++++N +EDEI ++V+  V  YK++  V F D+IPK+PSGK++R+ ++ ++
Sbjct: 502 SKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARL 550

BLAST of Sed0012565 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 396.4 bits (1017), Expect = 3.9e-110
Identity = 200/536 (37.31%), Postives = 323/536 (60.26%), Query Frame = 0

Query: 15  IFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYREVVRDTNRFAKALN 74
           IFRS+LP++ +P+ ++L D++ QN   +A     +   +G  YTY +V   + + A   +
Sbjct: 24  IFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFH 83

Query: 75  SLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEIKKQVEAADAKLVVT 134
            LG+ +  VV+++LPN  E+ +  L     G   +  NP    +EI KQ +A++ KL++T
Sbjct: 84  KLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIIT 143

Query: 135 NSANFEKVKEL----NLPVIVLDEE----LIEGSMNWYKLLEAADRAGNNFVKEDIKQTD 194
            +   +K+K L     + ++ +D+     + EG + + +L ++   A       +I   D
Sbjct: 144 EARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDD 203

Query: 195 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEI--EGKVTTLGLIPFFHIYGIT 254
           + ALP+SSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P FHIY + 
Sbjct: 204 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALN 263

Query: 255 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGL 314
            I    LR    +++M +F++   +  +   +VT AP+VPPI+LA+ K+   E++DLS +
Sbjct: 264 SIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSI 323

Query: 315 KLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAKKSTV 374
           ++  + + AAPL  EL+ A   KFP   + + YG+TE   +        KE    K    
Sbjct: 324 RV--VKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 383

Query: 375 GRILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGD 434
           G ++ N E+K ++PD+G SL +N PGEIC+R   +M+GY  N   T+ TID  GW+HTGD
Sbjct: 384 GTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGD 443

Query: 435 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAGEIPA 494
           IG IDDD ++FIVDR+KELIKYKGFQVAPAELEA+L+ H  + D AVV + +E AGE+P 
Sbjct: 444 IGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPV 503

Query: 495 ASVVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 541
           A VV ++++  +ED++ ++V+  V  YK++  V F ++IPK+PSGK++R+ ++ K+
Sbjct: 504 AFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

BLAST of Sed0012565 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 394.8 bits (1013), Expect = 1.1e-109
Identity = 208/531 (39.17%), Postives = 325/531 (61.21%), Query Frame = 0

Query: 15  IFRSQLPEVQVPDDITLPDFVLQNAESYAGNVAFVEAVSGKAYTYREVVRDTNRFAKALN 74
           IFRS+LP++ +P+ + L  +  +   S +     +   +GK+YTY E      R A  L 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 75  SLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISEIKKQVEAADAKLVVT 134
            LG+RKG V++++L N AE+    +G    G V +  NP     E+ KQ++++ AKL++T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 135 NSANFEKVKEL--NLPVIVLDEELIEGSMNWYKLLEAADRAGNNFVKE-DIKQTDLCALP 194
           +S   +K+K L  NL +I  DE   E  + +  L+   D   N F +  DI   D  ALP
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLI--TDDETNPFQETVDIGGDDAAALP 213

Query: 195 FSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--IEGKVTTLGLIPFFHIYGITGICCA 254
           FSSGTTG+ KGV+LTH++L+ ++   + G      ++     L ++P FHIY +  +   
Sbjct: 214 FSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLN 273

Query: 255 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAI 314
           +LR+   V++M +F++   ++ +    VT A +VPP+++AL KNP V  +DLS ++   +
Sbjct: 274 SLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRF--V 333

Query: 315 MTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSLGKENLAAKKSTVGRILP 374
           ++ AAPL  ELQ +  ++ P   + + YG+TE   +        KE +  K  + G ++ 
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 375 NLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYID 434
           N E+K ++ ++  SL  N PGEIC+R Q +M+ Y  + E TS TID +GW+HTGDIGY+D
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 435 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSVEDAAVVPLPDEEAGEIPAASVVM 494
           +D ++FIVDR+KE+IK+KGFQV PAELE++L+ H S+ DAAVVP  DE AGE+P A VV 
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 495 ARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKDKM 541
           +   + TE+++ +YVA  V  YK++  V FV +IPKSPSGK++R+ +K K+
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Sed0012565 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 384.8 bits (987), Expect = 1.2e-106
Identity = 200/554 (36.10%), Postives = 331/554 (59.75%), Query Frame = 0

Query: 10  EDEEH------IFRSQLPEVQVPDDITLPDFVLQ----NAESYAGNVAFVEAVSGKAYTY 69
           EDEE       IFRS+LP++ +P+ + L D+V Q    + +  +     ++  +G+  TY
Sbjct: 17  EDEEEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTY 76

Query: 70  REVVRDTNRFAKALNSLGLRKGQVVIVVLPNVAEYAIVALGIMATGGVFSGVNPAAHISE 129
            +V  +  R A  ++ LG+R G VV+++LPN  E+A+  L +   G V +  NP     E
Sbjct: 77  ADVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPE 136

Query: 130 IKKQVEAADAKLVVTNSANFEKVKEL---NLPVIVLDEE--------LIEGSMNWYKLLE 189
           I KQ +A+ AK+++T     +K+  L    + ++ LD++          +G +++ +L +
Sbjct: 137 IAKQAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQ 196

Query: 190 AADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEIE-- 249
           A +      +K  I   D  A+P+SSGTTG+ KGVM+TH+ LV ++   + G    +   
Sbjct: 197 ADE---TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFT 256

Query: 250 GKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPI 309
                L  +P FHIY +  +  + +R    ++++ RF+L   +  +   +VT  P+ PP+
Sbjct: 257 ANDVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPV 316

Query: 310 ILALVKNPIVEEFDLSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCIT 369
           +LA +K+P  E +DLS +++  +++ AA L  EL+ A   KFP     + YG+TE   + 
Sbjct: 317 VLAFIKSPETERYDLSSVRI--MLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTVA 376

Query: 370 LNYGSLGKENLAAKKSTVGRILPNLEVKFINPDSGRSLPKNTPGEICVRSQCVMQGYYKN 429
            +  +  K     K    G ++ N E+K ++ ++G SLP+N  GEICVR   +M+GY  +
Sbjct: 377 KSL-AFAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLND 436

Query: 430 EEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHSSV 489
            E T+RTID  GW+HTGDIG++DDD ++FIVDR+KELIK+KG+QVAPAELEA+L++H S+
Sbjct: 437 PEATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSI 496

Query: 490 EDAAVVPLPDEEAGEIPAASVVMARNANETEDEIIKYVASNVAHYKKVRLVHFVDTIPKS 541
           +DAAVV + DE A E+P A V  ++ +  TED++  YV   V HYK++++V F++ IPK+
Sbjct: 497 DDAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKA 556

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022945246.11.3e-29394.004-coumarate--CoA ligase-like 1 [Cucurbita moschata][more]
XP_023541569.12.5e-29293.644-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo][more]
KAG7012722.15.5e-29293.454-coumarate--CoA ligase-like 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_038893074.17.2e-29293.644-coumarate--CoA ligase-like 1 [Benincasa hispida][more]
XP_022966799.14.6e-29192.914-coumarate--CoA ligase-like 1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LQ123.2e-22673.684-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=... [more]
Q7XXL24.9e-20365.314-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9... [more]
P149121.3e-11539.634-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1[more]
I3PB372.3e-11539.294-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1[more]
P149132.3e-11539.814-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G0B46.3e-29494.004-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=... [more]
A0A6J1HUV52.2e-29192.914-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 ... [more]
A0A6J1DG055.2e-28892.364-coumarate--CoA ligase-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1DEE15.2e-28892.364-coumarate--CoA ligase-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A0A0KRZ51.0e-28390.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589320 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G62940.12.3e-22773.68acyl-CoA synthetase 5 [more]
AT3G21240.14.4e-11438.424-coumarate:CoA ligase 2 [more]
AT1G51680.13.9e-11037.314-coumarate:CoA ligase 1 [more]
AT1G65060.11.1e-10939.174-coumarate:CoA ligase 3 [more]
AT3G21230.11.2e-10636.104-coumarate:CoA ligase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 455..530
e-value: 9.2E-17
score: 61.8
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 13..441
e-value: 4.1E-131
score: 439.6
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 37..446
e-value: 1.6E-96
score: 323.5
NoneNo IPR availableGENE3D3.30.300.30coord: 442..544
e-value: 2.9E-30
score: 106.9
NoneNo IPR availablePANTHERPTHR24096:SF3894-COUMARATE--COA LIGASE-LIKE 1coord: 8..543
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 8..543
NoneNo IPR availableCDDcd059044CLcoord: 25..534
e-value: 0.0
score: 677.801
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 17..543
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 190..201

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0012565.1Sed0012565.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046949 fatty-acyl-CoA biosynthetic process
biological_process GO:0001676 long-chain fatty acid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004467 long-chain fatty acid-CoA ligase activity