Sed0012324 (gene) Chayote v1

BLAST of Sed0012324 vs. NCBI nr
Match: XP_038877318.1 (protein transport Sec1a-like [Benincasa hispida])

HSP 1 Score: 1174.1 bits (3036), Expect = 0.0e+00
Identity = 586/673 (87.07%), Postives = 629/673 (93.46%), Query Frame = 0

Query: 1   MSFSDSDV--GGNEYKNFRQTTRDRLLFEMLGAANTENSKPWKVLIMDRVTVKVMSHSCK 60
           MSFSDSD   G NEYKNFRQ +RDRLLFEMLGAANTENSKPWKVLIMD+VTVKVMSHSCK
Sbjct: 1   MSFSDSDTSSGPNEYKNFRQASRDRLLFEMLGAANTENSKPWKVLIMDKVTVKVMSHSCK 60

Query: 61  MADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVFFS 120
           MADITDQG+SLVEDLFRRRQPLPSMDAIYF+QPSKENVVMFLSDMSGREPLYKKAFVFFS
Sbjct: 61  MADITDQGVSLVEDLFRRRQPLPSMDAIYFIQPSKENVVMFLSDMSGREPLYKKAFVFFS 120

Query: 121 SPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFIGTENSRKF 180
           SPVPKEF+NHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQE AL DLF   ENSRKF
Sbjct: 121 SPVPKEFVNHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQERALEDLFGDIENSRKF 180

Query: 181 DNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNCVS 240
           DNCLNTMATRIATVFASLKEFPFVRY+ SKAL   +D T AS REL+P+KLAAAIWNC+S
Sbjct: 181 DNCLNTMATRIATVFASLKEFPFVRYRTSKAL---EDPTAASLRELVPTKLAAAIWNCIS 240

Query: 241 KYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSKTG 300
           KYK+TIPNYPQ+ETCELLILDRSIDQIAP+IHEWTYDAMCRDLLEM+GNKY+YEVSSKTG
Sbjct: 241 KYKTTIPNYPQSETCELLILDRSIDQIAPVIHEWTYDAMCRDLLEMDGNKYIYEVSSKTG 300

Query: 301 GEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEIST 360
           G PD+REA+LEDTDPVWLELRH+HIADASERLHEKMT F +KNKAAQIQQ+ARDGGEIST
Sbjct: 301 GAPDRREALLEDTDPVWLELRHSHIADASERLHEKMTNFVSKNKAAQIQQSARDGGEIST 360

Query: 361 RDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKEVI 420
           RDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLR+LGQLEQDLVFGDAGAK+VI
Sbjct: 361 RDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRDLGQLEQDLVFGDAGAKDVI 420

Query: 421 NFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLGGS 480
           +FLRT Q+ S ENKLRLLMIYASVYPEKFE+DKA+KIMQLAKLSTEDMK+VKNM+LL GS
Sbjct: 421 SFLRTNQNASPENKLRLLMIYASVYPEKFEDDKALKIMQLAKLSTEDMKVVKNMRLLAGS 480

Query: 481 DSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELPKSE 540
           DSKKA+S+HSFSLKF+ QKTKQA RKDRTGEEETWQLFRFYPM+EEL+E +CKGEL KSE
Sbjct: 481 DSKKASSAHSFSLKFNAQKTKQATRKDRTGEEETWQLFRFYPMVEELIENMCKGELSKSE 540

Query: 541 YSCMNEPPPSTEKPATKGTRITASQTSQSTGGPKSMRSRRTANWARTSVSDDGYGSDSVL 600
           YSCMNEPPP TEK A KGT+   SQT QSTGGPKSMRSRRTANWAR+S+SDDGYGSDS+L
Sbjct: 541 YSCMNEPPPVTEKAAPKGTQSATSQTGQSTGGPKSMRSRRTANWARSSISDDGYGSDSIL 600

Query: 601 RHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKLKQ 660
           R    D K+MG+RVFVF+IGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKLK 
Sbjct: 601 RAATLDFKKMGQRVFVFIIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKLKL 660

Query: 661 LSEKDLQTEPPRF 672
           LSE+D+  E PRF
Sbjct: 661 LSERDISVEAPRF 670

BLAST of Sed0012324 vs. NCBI nr
Match: XP_008460124.1 (PREDICTED: protein transport Sec1a-like isoform X1 [Cucumis melo])

HSP 1 Score: 1171.8 bits (3030), Expect = 0.0e+00
Identity = 585/675 (86.67%), Postives = 630/675 (93.33%), Query Frame = 0

Query: 1   MSFSDSD---VGG-NEYKNFRQTTRDRLLFEMLGAANTENSKPWKVLIMDRVTVKVMSHS 60
           MSFSDSD   +GG NEYKNFRQT+RDRLL+EMLGAANTENSKPWKVLIMD+VTVKVMSHS
Sbjct: 1   MSFSDSDSCSIGGHNEYKNFRQTSRDRLLYEMLGAANTENSKPWKVLIMDKVTVKVMSHS 60

Query: 61  CKMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVF 120
           CKMADITDQG+SLVEDLFRRRQPLPSMDAIYF+QPSKENVVMFLSDMSGREPLYKKAFVF
Sbjct: 61  CKMADITDQGVSLVEDLFRRRQPLPSMDAIYFIQPSKENVVMFLSDMSGREPLYKKAFVF 120

Query: 121 FSSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFIGTENSR 180
           FSSPVPKEF+NHIKCDTSVLPRIGALREMNLEYFPIDSQAF TDQE AL DLF   ENSR
Sbjct: 121 FSSPVPKEFVNHIKCDTSVLPRIGALREMNLEYFPIDSQAFFTDQERALEDLFGDIENSR 180

Query: 181 KFDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNC 240
           KFDNCLNTMATRIATVFASLKEFPFVRY+ SKAL   DD TEAS REL+P+KLAAAIWNC
Sbjct: 181 KFDNCLNTMATRIATVFASLKEFPFVRYRASKAL---DDPTEASLRELVPTKLAAAIWNC 240

Query: 241 VSKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSK 300
           +SKYK+TIPNYPQ+ETCELLILDRSIDQIAP+IHEWTYDAMCRDLLEM+GNKY YEVSSK
Sbjct: 241 ISKYKTTIPNYPQSETCELLILDRSIDQIAPVIHEWTYDAMCRDLLEMDGNKYTYEVSSK 300

Query: 301 TGGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEI 360
           TGG PDKREA+LEDTDPVWLELRH+HIADASERLHEKMT F +KNKAAQIQQ+ARDGGEI
Sbjct: 301 TGGAPDKREALLEDTDPVWLELRHSHIADASERLHEKMTNFVSKNKAAQIQQSARDGGEI 360

Query: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKE 420
           STRDLQKMVQALPQYTEQVEKITLHVEIAGK+NKLIRE+GLR+LGQLEQDLVFGDAGAK+
Sbjct: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKVNKLIRELGLRDLGQLEQDLVFGDAGAKD 420

Query: 421 VINFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLG 480
           VIN+LRT Q+ S ENKLRLLMIYASVYPEKFE+DKA+KIMQLAKLSTEDMK+VKNM+LL 
Sbjct: 421 VINYLRTNQNASPENKLRLLMIYASVYPEKFEDDKALKIMQLAKLSTEDMKVVKNMRLLA 480

Query: 481 GSDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELPK 540
           GSDSKK +S+HSFSLKF+ QKTKQA RKDRTGEEETWQLFRFYPMIEEL+E LCKG+L K
Sbjct: 481 GSDSKKTSSAHSFSLKFNAQKTKQATRKDRTGEEETWQLFRFYPMIEELIENLCKGDLSK 540

Query: 541 SEYSCMNEPPPSTEKPATKGTRITASQTSQSTGGPKSMRSRRTANWARTSVSDDGYGSDS 600
           SEYSCMNEPPP TEKPA KG++   SQT QSTGGPKSMRSRRTANWAR+S+SDDGYGSDS
Sbjct: 541 SEYSCMNEPPPVTEKPAPKGSQSATSQTGQSTGGPKSMRSRRTANWARSSISDDGYGSDS 600

Query: 601 VLRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKL 660
           +LR    D K+MG+RVFVF+IGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKL
Sbjct: 601 ILRAATLDFKKMGQRVFVFIIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKL 660

Query: 661 KQLSEKDLQTEPPRF 672
           K L+E+ +  E PRF
Sbjct: 661 KLLTERGISVEAPRF 672

BLAST of Sed0012324 vs. NCBI nr
Match: XP_004153750.1 (protein transport Sec1a isoform X1 [Cucumis sativus] >KGN46241.1 hypothetical protein Csa_004776 [Cucumis sativus])

HSP 1 Score: 1167.9 bits (3020), Expect = 0.0e+00
Identity = 582/675 (86.22%), Postives = 629/675 (93.19%), Query Frame = 0

Query: 1   MSFSDSD---VGG-NEYKNFRQTTRDRLLFEMLGAANTENSKPWKVLIMDRVTVKVMSHS 60
           MSFSDSD   +GG NEYKNFRQT+RDRLL+EMLGAANTENSKPWKVLIMD+VTVKVMSHS
Sbjct: 1   MSFSDSDSSSIGGHNEYKNFRQTSRDRLLYEMLGAANTENSKPWKVLIMDKVTVKVMSHS 60

Query: 61  CKMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVF 120
           CKMADITDQG+SLVEDLFRRRQPLPSMDAIYF+QPSKENVVMFLSDMSGREPLYKKAFVF
Sbjct: 61  CKMADITDQGVSLVEDLFRRRQPLPSMDAIYFIQPSKENVVMFLSDMSGREPLYKKAFVF 120

Query: 121 FSSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFIGTENSR 180
           FSSPVPKEF+NHIKCDTSVLPRIGALREMNLEYFPIDSQAF TDQE AL DLF   ENSR
Sbjct: 121 FSSPVPKEFVNHIKCDTSVLPRIGALREMNLEYFPIDSQAFFTDQERALEDLFGDIENSR 180

Query: 181 KFDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNC 240
           KFDNCLNTMATRIATVFASLKEFPFV+Y+ SKAL   DD TEAS REL+P+KLAAAIWNC
Sbjct: 181 KFDNCLNTMATRIATVFASLKEFPFVKYRASKAL---DDPTEASLRELVPTKLAAAIWNC 240

Query: 241 VSKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSK 300
           +SKYK+TIPNYPQ+ETCELLILDRSIDQIAP+IHEWTYDAMCRDLLEM+GNKY YEVSSK
Sbjct: 241 ISKYKTTIPNYPQSETCELLILDRSIDQIAPVIHEWTYDAMCRDLLEMDGNKYTYEVSSK 300

Query: 301 TGGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEI 360
           TGG PD+REA+LEDTDPVWLELRH+HIADASERLHEKMT F +KNKAAQIQQNARDGGE+
Sbjct: 301 TGGAPDRREALLEDTDPVWLELRHSHIADASERLHEKMTNFVSKNKAAQIQQNARDGGEV 360

Query: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKE 420
           STRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIRE+GLR+LGQLEQDLVFGDAGAK+
Sbjct: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIRELGLRDLGQLEQDLVFGDAGAKD 420

Query: 421 VINFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLG 480
           VIN+LRT Q+ S ENKLRLLMIYASVYPEKFE+DKA+KIMQLAKLSTEDMK+VKNM+LLG
Sbjct: 421 VINYLRTNQNASPENKLRLLMIYASVYPEKFEDDKALKIMQLAKLSTEDMKVVKNMRLLG 480

Query: 481 GSDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELPK 540
           GSDSKKA+S HSFSLKF+ QKTKQA RKDRTGEEETWQLFRFYPMIEEL+E LCKG+L K
Sbjct: 481 GSDSKKASSGHSFSLKFNAQKTKQATRKDRTGEEETWQLFRFYPMIEELIENLCKGDLSK 540

Query: 541 SEYSCMNEPPPSTEKPATKGTRITASQTSQSTGGPKSMRSRRTANWARTSVSDDGYGSDS 600
           SEYSC+NEPPP TEK   KG++   SQT QSTGGPKSMRSRRTANWAR+S+SDDGYGSDS
Sbjct: 541 SEYSCINEPPPVTEKAPPKGSQSATSQTGQSTGGPKSMRSRRTANWARSSISDDGYGSDS 600

Query: 601 VLRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKL 660
           +L+    D K+MG+RVFVF++GGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKL
Sbjct: 601 ILKAATLDFKKMGQRVFVFIVGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKL 660

Query: 661 KQLSEKDLQTEPPRF 672
           K L+EK +  E PRF
Sbjct: 661 KLLTEKGISVEAPRF 672

BLAST of Sed0012324 vs. NCBI nr
Match: XP_023514794.1 (protein transport Sec1a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1154.0 bits (2984), Expect = 0.0e+00
Identity = 580/678 (85.55%), Postives = 625/678 (92.18%), Query Frame = 0

Query: 1   MSFSDSD---VGG-NEYKNFRQTTRDRLLFEMLGAANTENSKPWKVLIMDRVTVKVMSHS 60
           MSFSDSD   VGG NEYKNFRQT+RDRLLFEMLGAANTENSKPW+VLIMD+VTVKVMSHS
Sbjct: 1   MSFSDSDSSSVGGTNEYKNFRQTSRDRLLFEMLGAANTENSKPWRVLIMDKVTVKVMSHS 60

Query: 61  CKMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVF 120
           CKMADITDQG+SLVEDLFRRRQPLPS+DAIYF+QPSKENVVMFLSDMSGREPLYKKAFVF
Sbjct: 61  CKMADITDQGVSLVEDLFRRRQPLPSLDAIYFIQPSKENVVMFLSDMSGREPLYKKAFVF 120

Query: 121 FSSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFIGTENSR 180
           FSSPVP+EF+NHIKCDTSVLPRIGALREMNLEYFPIDSQAFITD + AL DLF+ +ENSR
Sbjct: 121 FSSPVPREFVNHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDHDRALDDLFVESENSR 180

Query: 181 KFDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNC 240
           +FDNCLNTMATRIATVFASLKEFPFVRY+ SKAL   +D T AS REL+P+KLAAA+WNC
Sbjct: 181 RFDNCLNTMATRIATVFASLKEFPFVRYRASKAL---EDPTAASLRELVPTKLAAAVWNC 240

Query: 241 VSKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSK 300
           +SKYKSTIPNYPQ+ETCELLILDRSIDQIAP+IHEWTYDAMCRDLLEMEGNKY+YEVSSK
Sbjct: 241 ISKYKSTIPNYPQSETCELLILDRSIDQIAPVIHEWTYDAMCRDLLEMEGNKYIYEVSSK 300

Query: 301 TGGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEI 360
           TGG+PDKREA+LEDTDPVWLELRH HIADASERLHEKMTTF +KNKAAQI Q ARDGGEI
Sbjct: 301 TGGDPDKREALLEDTDPVWLELRHAHIADASERLHEKMTTFASKNKAAQIHQTARDGGEI 360

Query: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKE 420
           STRDLQKMVQALPQYTEQVEKITLHVEIAGKIN LIREMGLRELGQLEQDLVFGDAGAK+
Sbjct: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINTLIREMGLRELGQLEQDLVFGDAGAKD 420

Query: 421 VINFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLG 480
           VINFLRT Q+ S ENKLRLLMIYASVYPEKFE+DKA KIMQLAKL+ EDM +VKNM+LL 
Sbjct: 421 VINFLRTNQNASPENKLRLLMIYASVYPEKFEDDKAAKIMQLAKLTAEDMTVVKNMRLLA 480

Query: 481 GSDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELPK 540
           GSDSKKATS+HSFSLKF+ QKTKQA RKDRTGEEETWQLFRFYPMIEELVE LCKGEL K
Sbjct: 481 GSDSKKATSAHSFSLKFNAQKTKQATRKDRTGEEETWQLFRFYPMIEELVENLCKGELSK 540

Query: 541 SEYSCMNEPPPSTEKPATK--GTRITASQTSQSTGGPKSMRSRRTANWARTSVSDDGYGS 600
           +EYSCMNEP PST KPA     T+   SQTSQS GGPKSMRSRRTANWAR+S+SDDGYGS
Sbjct: 541 NEYSCMNEPSPSTAKPAVPRGSTQSATSQTSQSAGGPKSMRSRRTANWARSSISDDGYGS 600

Query: 601 DSVLRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYIT 660
           DS+L+    D K+MG+RVFVF+IGGATRSELRVCHKLTAKLRREVVLGCS+LDDPPQYI 
Sbjct: 601 DSILKAATLDFKKMGQRVFVFIIGGATRSELRVCHKLTAKLRREVVLGCSTLDDPPQYIM 660

Query: 661 KLKQLSEKDLQTEPPRFF 673
           KLK+LSE +   E P+FF
Sbjct: 661 KLKRLSEPEPPVEAPKFF 675

BLAST of Sed0012324 vs. NCBI nr
Match: XP_023000174.1 (protein transport Sec1a-like [Cucurbita maxima])

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 578/678 (85.25%), Postives = 625/678 (92.18%), Query Frame = 0

Query: 1   MSFSDSD---VGG-NEYKNFRQTTRDRLLFEMLGAANTENSKPWKVLIMDRVTVKVMSHS 60
           MSFSDSD   VGG NEYKNFRQT+RDRLLFEMLGAANTENSKPW+VLIMD+VTVKVMSHS
Sbjct: 1   MSFSDSDSSSVGGTNEYKNFRQTSRDRLLFEMLGAANTENSKPWRVLIMDKVTVKVMSHS 60

Query: 61  CKMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVF 120
           CKMADITDQG+SLVEDLFRRRQPLPS+DAIYF+QPSKENVVMFLSDMSGREPLYKKAFVF
Sbjct: 61  CKMADITDQGVSLVEDLFRRRQPLPSLDAIYFIQPSKENVVMFLSDMSGREPLYKKAFVF 120

Query: 121 FSSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFIGTENSR 180
           FSSPVP+EF+NHIKCDTSVLPRIGALREMNLEYFPIDSQAFITD + AL DLF+ +ENSR
Sbjct: 121 FSSPVPREFVNHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDHDRALDDLFVESENSR 180

Query: 181 KFDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNC 240
           +FDNCLNTMATRIATVFASLKEFPFVRY+ SKAL   +D T AS REL+P+KLAAA+WNC
Sbjct: 181 RFDNCLNTMATRIATVFASLKEFPFVRYRASKAL---EDPTAASLRELVPTKLAAAVWNC 240

Query: 241 VSKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSK 300
           +SKYKSTIPNYPQ+ETCELLILDRSIDQIAP+IHEWTYDAMCRDLLEMEGNKY+YEVSSK
Sbjct: 241 ISKYKSTIPNYPQSETCELLILDRSIDQIAPVIHEWTYDAMCRDLLEMEGNKYIYEVSSK 300

Query: 301 TGGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEI 360
           TGG+PDKREA+LEDTDPVWLELRH HIADASERLHEKMTTF +KNKAAQI Q ARDGGEI
Sbjct: 301 TGGDPDKREALLEDTDPVWLELRHAHIADASERLHEKMTTFASKNKAAQIHQTARDGGEI 360

Query: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKE 420
           STRDLQKMVQALPQYTEQVEKITLHVEIAGKIN LIREMGLRELGQLEQDLVFGDAGAK+
Sbjct: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINTLIREMGLRELGQLEQDLVFGDAGAKD 420

Query: 421 VINFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLG 480
           VINFLRT Q+ S ENKLRLLMIYASVYPEKFE+DKA KIMQLAKL+ EDM +VKNM+LL 
Sbjct: 421 VINFLRTNQNASPENKLRLLMIYASVYPEKFEDDKAAKIMQLAKLTAEDMTVVKNMRLLA 480

Query: 481 GSDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELPK 540
           GSD+KKATS+HSFSLKF+ QKTKQA RKDRTGEEETWQLFRFYPMIEEL+E LCKGEL K
Sbjct: 481 GSDAKKATSAHSFSLKFNAQKTKQATRKDRTGEEETWQLFRFYPMIEELIENLCKGELSK 540

Query: 541 SEYSCMNEPPPSTEKPATK--GTRITASQTSQSTGGPKSMRSRRTANWARTSVSDDGYGS 600
           +EYSCMNEP PST KPA     T+   SQTSQS GGPKSMRSRRTANWAR+S+SDDGYGS
Sbjct: 541 NEYSCMNEPSPSTTKPAVPRGSTQSATSQTSQSAGGPKSMRSRRTANWARSSISDDGYGS 600

Query: 601 DSVLRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYIT 660
           DS+L+    D K+MG+RVFVF+IGGATRSELRVCHKLTAKLRREVVLGCS+LDDPPQYI 
Sbjct: 601 DSILKAATLDFKKMGQRVFVFIIGGATRSELRVCHKLTAKLRREVVLGCSTLDDPPQYIM 660

Query: 661 KLKQLSEKDLQTEPPRFF 673
           KLK+LSE +   E P+FF
Sbjct: 661 KLKRLSEPEPPVEAPKFF 675

BLAST of Sed0012324 vs. ExPASy Swiss-Prot
Match: Q9C5P7 (Protein transport Sec1a OS=Arabidopsis thaliana OX=3702 GN=SEC1A PE=2 SV=3)

HSP 1 Score: 931.8 bits (2407), Expect = 4.4e-270
Identity = 478/681 (70.19%), Postives = 561/681 (82.38%), Query Frame = 0

Query: 1   MSFSDSDV-----GGNEYKNFRQTTRDRLLFEMLGAANTENSKPWKVLIMDRVTVKVMSH 60
           MSFSDS+      GG +YK FRQ +RDRLL EMLG+  T +SK WK+LIMDRVTVKVMS 
Sbjct: 1   MSFSDSESSSHGGGGGDYKFFRQISRDRLLHEMLGSTKTGDSKAWKILIMDRVTVKVMSQ 60

Query: 61  SCKMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFV 120
           SCKMADITDQGISLVE+LF+RR+P+P MDAIYF+QPSKEN+VMFLSDMSGREPLY+KAF+
Sbjct: 61  SCKMADITDQGISLVEELFKRREPMPGMDAIYFIQPSKENIVMFLSDMSGREPLYRKAFI 120

Query: 121 FFSSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFI-GTEN 180
           FFSS +PKE +NHIK D+SVLPRIGALREMN+EYFPID+Q F+TD E AL  L+    EN
Sbjct: 121 FFSSTIPKELVNHIKSDSSVLPRIGALREMNMEYFPIDNQGFLTDHEQALETLYAEDAEN 180

Query: 181 SRKFDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIW 240
           SR F  CLN MATRIATVFASLKE PFVRY+ +K+          + R+L+PSKLAAAIW
Sbjct: 181 SRHFHICLNIMATRIATVFASLKELPFVRYRAAKS---------TASRDLVPSKLAAAIW 240

Query: 241 NCVSKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVS 300
           +C+SKYK+ IPN+PQ ETCELLI+DRS+DQIAPIIHEWTYDAMC DLL+MEGNK+V EV 
Sbjct: 241 DCISKYKA-IPNFPQTETCELLIVDRSVDQIAPIIHEWTYDAMCHDLLDMEGNKHVIEVP 300

Query: 301 SKTGGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGG 360
           SKTGG P+K+E +LED DPVWLELRHTHIADASERLHEKMT F +KNKAAQ++  +RDG 
Sbjct: 301 SKTGGPPEKKEIVLEDHDPVWLELRHTHIADASERLHEKMTNFASKNKAAQMR--SRDGS 360

Query: 361 EISTRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGA 420
           E+STRDLQK+VQALPQY EQV+K++ HVE+AGKIN++IR+ GLR+LGQLEQDLVFGDAGA
Sbjct: 361 ELSTRDLQKIVQALPQYGEQVDKLSTHVELAGKINRIIRDTGLRDLGQLEQDLVFGDAGA 420

Query: 421 KEVINFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQL 480
           K+VINFLRT Q  + ENKLRLLMIYA+VYPEKFE DK VK+MQLA+LS  DMK++ NMQL
Sbjct: 421 KDVINFLRTNQDTNPENKLRLLMIYATVYPEKFEGDKGVKLMQLARLSPVDMKVISNMQL 480

Query: 481 LGGSDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGEL 540
           + GS   KA S  SFSLKF   KTKQA RKDR+GEEETWQLFRFYPMIEEL+EKL KG+L
Sbjct: 481 IAGSPENKAKSG-SFSLKFDAGKTKQANRKDRSGEEETWQLFRFYPMIEELLEKLVKGDL 540

Query: 541 PKSEYSCMNEPPPSTEKPATKGT-RITASQTS--QSTGGPKSMRSRRTANWARTSVSDDG 600
            KS+Y CMN+     E  A  G+ R +++ T+  +    P SMRSRRTA WAR   SDDG
Sbjct: 541 SKSDYLCMNQSSHKEESEARTGSVRKSSAPTAVPERKATPHSMRSRRTATWARPHSSDDG 600

Query: 601 YGSDSVLRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQ 660
           Y SDSVL+  + + K++G+R+FVF+IGGATRSELRVCHKLT+ LRREVVLG +S DDPPQ
Sbjct: 601 YSSDSVLKSASTEFKKLGQRIFVFIIGGATRSELRVCHKLTSSLRREVVLGSTSFDDPPQ 660

Query: 661 YITKLKQLSEKDLQTEPPRFF 673
           YITKLK LSEKD+Q  P + F
Sbjct: 661 YITKLKLLSEKDIQGAPAQPF 668

BLAST of Sed0012324 vs. ExPASy Swiss-Prot
Match: Q9C5X3 (SNARE-interacting protein KEULE OS=Arabidopsis thaliana OX=3702 GN=KEU PE=1 SV=2)

HSP 1 Score: 880.9 bits (2275), Expect = 8.9e-255
Identity = 443/666 (66.52%), Postives = 537/666 (80.63%), Query Frame = 0

Query: 1   MSFSDSDVG--GNEYKNFRQTTRDRLLFEMLGAANTENSK-PWKVLIMDRVTVKVMSHSC 60
           MS+SDSD    G EYKNFRQ TR+RLL+EML +A T +SK  WKVLIMD++TVK+MS++C
Sbjct: 1   MSYSDSDSSSHGGEYKNFRQITRERLLYEMLRSAKTGSSKSTWKVLIMDKLTVKIMSYAC 60

Query: 61  KMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVFF 120
           KMADIT +G+SLVED+FRRRQPLPSMDAIYF+QP+KENV+MFLSDMSG+ PLYKKAFVFF
Sbjct: 61  KMADITQEGVSLVEDIFRRRQPLPSMDAIYFIQPTKENVIMFLSDMSGKSPLYKKAFVFF 120

Query: 121 SSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFIGTENSRK 180
           SSPV KE + HIK D+SVLPRIGALREMNLE+F IDSQ FITD E AL DLF   E SRK
Sbjct: 121 SSPVSKELVGHIKKDSSVLPRIGALREMNLEFFAIDSQGFITDHERALEDLFGDEETSRK 180

Query: 181 FDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNCV 240
            D CLN MA+RIATVFASL+EFP VRY+ +K+LD     T  + R+LIP+KLAA IWNC+
Sbjct: 181 GDACLNVMASRIATVFASLREFPAVRYRAAKSLDAS---TMTTLRDLIPTKLAAGIWNCL 240

Query: 241 SKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSKT 300
           +K+K +I N+PQ ETCELLILDRSIDQIAP+IHEWTYDAMC DLL MEGNKYV+ + SK+
Sbjct: 241 AKHKQSIENFPQTETCELLILDRSIDQIAPVIHEWTYDAMCHDLLNMEGNKYVHVIPSKS 300

Query: 301 GGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEIS 360
           GG+P+K++ +LE+ DP+WLELRH HIADASERLH+KMT F +KNKAAQ+ Q  RDG E+S
Sbjct: 301 GGQPEKKDVLLEEHDPIWLELRHAHIADASERLHDKMTNFLSKNKAAQL-QGKRDGAELS 360

Query: 361 TRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKEV 420
           TRDLQKMVQALPQY+EQ++K++LHVEIA K+N LIRE GLRELGQLEQDLVFGDAG K+V
Sbjct: 361 TRDLQKMVQALPQYSEQIDKLSLHVEIARKLNDLIREQGLRELGQLEQDLVFGDAGMKDV 420

Query: 421 INFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLGG 480
           I +L T +  S E KLRLLMI A++YPEKFE +K   +M+LAKLS++DM  V NM LLG 
Sbjct: 421 IKYLSTQEEASREGKLRLLMILATIYPEKFEGEKGQNLMKLAKLSSDDMTAVNNMSLLGS 480

Query: 481 SDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELPKS 540
           +   K  +   F+LKF   K K+A RK+R  EE  WQL RFYPMIEEL+EKL KGELPK 
Sbjct: 481 AVDAKKNTPGGFTLKFDLHKKKRAVRKERQ-EEAAWQLSRFYPMIEELIEKLSKGELPKE 540

Query: 541 EYSCMNEPPPSTEKPATKGTRITASQTSQSTGGPKSMRSRRTANWARTSVSDDGYGSDSV 600
           ++ CMN+P PS        T ++++ +S      +SMRSRRT  WA+   SDDGY SDSV
Sbjct: 541 DFPCMNDPSPSFH----GSTSLSSAASSSQGQAAQSMRSRRTPTWAKPRGSDDGYSSDSV 600

Query: 601 LRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKLK 660
           LRH + D ++MG+R+FVF++GGATRSEL+VCHKL+ KL+REV+LG +SLDDPPQ+ITKLK
Sbjct: 601 LRHASSDFRKMGQRIFVFIVGGATRSELKVCHKLSTKLKREVILGSTSLDDPPQFITKLK 657

Query: 661 QLSEKD 664
            L+  D
Sbjct: 661 LLTAND 657

BLAST of Sed0012324 vs. ExPASy Swiss-Prot
Match: Q7XWP3 (Probable protein transport Sec1a OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0252400 PE=3 SV=2)

HSP 1 Score: 804.7 bits (2077), Expect = 8.1e-232
Identity = 405/661 (61.27%), Postives = 516/661 (78.06%), Query Frame = 0

Query: 6   SDVGGNEYKNFRQTTRDRLLFEMLGAANTENSKPWKVLIMDRVTVKVMSHSCKMADITDQ 65
           S   G +Y++FRQ TRDRLLFEML +    +   WKVLIMD++TVK+MS SCKMAD+ ++
Sbjct: 8   SSRSGADYRSFRQITRDRLLFEMLRSTKKSSKSAWKVLIMDKLTVKIMSFSCKMADVMEE 67

Query: 66  GISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVFFSSPVPKEF 125
           G+SLVEDL+ RRQPLP MDAIYF+QP+KEN+ +F+SDMSG+ PLYKKA+VFFSSPV +E 
Sbjct: 68  GVSLVEDLYMRRQPLPLMDAIYFIQPTKENIRIFMSDMSGKIPLYKKAYVFFSSPVQREL 127

Query: 126 INHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFI-GTENSRKFDNCLNT 185
           +  IK D++V  RIGAL EMNLEYF IDSQ F TD + AL +LF    E S K+++CLN 
Sbjct: 128 VAQIKKDSNVRARIGALSEMNLEYFAIDSQGFTTDHDKALEELFSENAEGSLKYNSCLNM 187

Query: 186 MATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNCVSKYKSTI 245
           MATRIATVFAS++EFP V Y+V++ +D     T  + R+L P+KLAA +WNC++++K+ I
Sbjct: 188 MATRIATVFASMREFPRVHYRVARTIDAS---TLTTLRDLAPTKLAAGVWNCLARFKAMI 247

Query: 246 PNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSKTGGEPDKR 305
           P +PQ ETCELLI+DRSIDQIAPIIHEWTYDAMC DLL M+GNKYV +V SK+G   + +
Sbjct: 248 PEFPQTETCELLIVDRSIDQIAPIIHEWTYDAMCHDLLCMDGNKYVQQVPSKSGSGTENK 307

Query: 306 EAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEISTRDLQKM 365
           E +LED DP+WLELRH HIA+ASERLHEKMT F +KNKAAQ+ Q AR+GG++ST++LQKM
Sbjct: 308 EVLLEDHDPIWLELRHVHIANASERLHEKMTNFVSKNKAAQLHQ-ARNGGDLSTKELQKM 367

Query: 366 VQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKEVINFLRTY 425
           VQALPQY++Q++K+ LHVEIAGK+N  I+E  L+++GQLEQDLVFGDAG KE+INF RT+
Sbjct: 368 VQALPQYSDQIDKLALHVEIAGKLNSTIKEQQLKDVGQLEQDLVFGDAGTKELINFFRTH 427

Query: 426 QSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLGGSDSKKAT 485
              S ENKLRLLM+YA++ P+K  +DK  K+MQLA LS +DM  V NM+ L G DSKK +
Sbjct: 428 LDISRENKLRLLMVYAAINPDKTRSDKGAKLMQLAGLSADDMIAVSNMRCLCGHDSKK-S 487

Query: 486 SSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELPKSEYSCMNE 545
           S+  F+LKF  +K +   RK+R GEE  W L RFYP++EEL+EKL KGELPK EY  +N+
Sbjct: 488 SAGGFTLKFDLRKKRHGIRKERIGEESKWMLSRFYPILEELIEKLSKGELPKDEYHYLND 547

Query: 546 PPPSTEKPATKGTRITASQTSQSTGGPKSMRSRRT-ANWARTSVSDDGYGSDSVLRHINF 605
           P PS      +G    ++QTS +    +SMRSRRT   WAR   SDDGY SDSVL+H + 
Sbjct: 548 PSPS-----FRGIPSASTQTSPAHQPAQSMRSRRTGGTWARPRDSDDGYSSDSVLKHTSS 607

Query: 606 DSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKLKQLSEKD 665
           +S+++G+R+FVFVIGGATRSEL   HKL++KL+RE++LG SSLDDPPQ+ITKLK LS  D
Sbjct: 608 NSRKLGQRLFVFVIGGATRSELCAAHKLSSKLKREIILGSSSLDDPPQFITKLKMLSTDD 658

BLAST of Sed0012324 vs. ExPASy Swiss-Prot
Match: Q5VNU3 (Probable protein transport Sec1b OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0135900 PE=2 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 8.1e-232
Identity = 416/664 (62.65%), Postives = 520/664 (78.31%), Query Frame = 0

Query: 1   MSFSDSDVGG--NEYKNFRQTTRDRLLFEMLGA-ANTENSKPWKVLIMDRVTVKVMSHSC 60
           MS S SD G   ++ K FR   RDR+L ++L    + E    WKVLIMD+ TV++M+++C
Sbjct: 1   MSMSGSDFGAPCDDPKIFRNICRDRILKDLLQPDKDKETKSSWKVLIMDKFTVRIMAYAC 60

Query: 61  KMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVFF 120
           KMA+ITD GISLVEDLF+RR+P+PSMDAIYF+QP KENV+M LSDMSGR PLY+KA++FF
Sbjct: 61  KMAEITDAGISLVEDLFKRREPMPSMDAIYFLQPLKENVIMLLSDMSGRCPLYRKAYIFF 120

Query: 121 SSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFIGTENSRK 180
           SSP+PKE +++IK D+SV+PRIGALREMNLE+F ID Q F TD +MA  DL+    NS+K
Sbjct: 121 SSPIPKELVSYIKNDSSVIPRIGALREMNLEFFAIDMQGFTTDHDMAFTDLYSAQHNSKK 180

Query: 181 FDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNCV 240
           F++ ++TMATRIAT FASLKEFP VRY+  K     D +T   F +++P  LA A+W+ V
Sbjct: 181 FNDTISTMATRIATTFASLKEFPCVRYRAPK---GTDPMTTPKF-DMVPKWLATAVWDIV 240

Query: 241 SKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSKT 300
           SKYKSTIP +PQ ETCELLI+DR IDQIAP+IHEWTYDAMC DLLEM+G KY+YEV SK 
Sbjct: 241 SKYKSTIPEFPQKETCELLIVDRPIDQIAPVIHEWTYDAMCHDLLEMDGQKYIYEV-SKA 300

Query: 301 GGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEIS 360
           G EP+++EA+LED DP+W+ELRH HIADASERL++KM  F +KNKAAQ+  ++RDGGEIS
Sbjct: 301 GSEPERKEALLEDHDPLWVELRHIHIADASERLYDKMNNFVSKNKAAQL--HSRDGGEIS 360

Query: 361 TRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKEV 420
           T+DLQK+VQALPQY EQVEK+TLH+EIAGKINK IRE GLR++GQ+EQDLVFGDA AKEV
Sbjct: 361 TKDLQKIVQALPQYGEQVEKLTLHIEIAGKINKFIREYGLRDIGQVEQDLVFGDAAAKEV 420

Query: 421 INFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLGG 480
           I+ LR+ Q  S ENKLRLL+IYA VYPEKFE DK  K+MQLAKL  ++M  + +++ L G
Sbjct: 421 ISILRSKQDMSPENKLRLLIIYAIVYPEKFEGDKGEKLMQLAKLPHDEMDAINSLRYLVG 480

Query: 481 SDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELPKS 540
           SD+KKA+    FSLKF  QK K AAR +R   +ETW L RF+P+IEEL+EKL KG LP +
Sbjct: 481 SDTKKASRPGGFSLKFDAQKKKNAARTERQDGDETWALSRFFPLIEELIEKLSKGALPLN 540

Query: 541 EYSCMNEPPPSTEKPATKGTRITASQTSQSTGGPKSMRSRRTANWARTSVSDDGYGSD-S 600
           EY  M+E P STE+ +T+    +A+ T  +   P S RSRRT  WA++  SDD   SD S
Sbjct: 541 EYPSMSE-PSSTEQGSTQ----SAAATKPAQAQPMSRRSRRTPTWAKSRNSDDSQSSDSS 600

Query: 601 VLRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKL 660
           VLRH + D K++G R+FVF+IGGATRSELR  HKLT KL+RE+VLG SS+DDPPQ+I+KL
Sbjct: 601 VLRHGSNDFKRLGNRIFVFMIGGATRSELRTVHKLTMKLKREIVLGSSSIDDPPQFISKL 652

BLAST of Sed0012324 vs. ExPASy Swiss-Prot
Match: Q9SZ77 (Protein transport Sec1b OS=Arabidopsis thaliana OX=3702 GN=SEC1B PE=2 SV=3)

HSP 1 Score: 781.2 bits (2016), Expect = 9.6e-225
Identity = 399/668 (59.73%), Postives = 508/668 (76.05%), Query Frame = 0

Query: 1   MSFSDSDVG--GNEYKNFRQTTRDRLLFEMLGAANTENSK-PWKVLIMDRVTVKVMSHSC 60
           MSFSDS     G EYKNFRQ TR+RLL EML      +SK  WKVL+MD+ TVK+MS +C
Sbjct: 1   MSFSDSGSSSYGGEYKNFRQITRERLLCEMLRPERNGSSKLTWKVLVMDKFTVKIMSSAC 60

Query: 61  KMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVFF 120
           KM++IT +GISLVE + + RQP+ +M+ IYF+QP++ENV  FLSDM+G+ PLYKKAFVFF
Sbjct: 61  KMSEITQEGISLVEVITKHRQPMTAMEVIYFIQPTEENVTAFLSDMTGKSPLYKKAFVFF 120

Query: 121 SSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFIGTENSRK 180
           SSPV +  +N IK D   + RIG L+EMNLEY  +D Q F+T+ E AL +LF   EN ++
Sbjct: 121 SSPVSRSLVNLIKKDMRAMKRIGGLKEMNLEYISMDIQGFVTNNENALEELFCDDENHQR 180

Query: 181 FDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNCV 240
            D CLN +A RIATV ASLKE+PFVRY+ +KAL   D  T  ++RELIP+KLAA++WNC+
Sbjct: 181 ADACLNVVAKRIATVLASLKEYPFVRYRGAKAL---DATTMTTYRELIPTKLAASVWNCL 240

Query: 241 SKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSKT 300
           ++YK TI ++PQ ETCELLILDRSIDQIAP+IHEWTYDAMC DLL MEGNKY +EV SKT
Sbjct: 241 ARYKQTIEDFPQTETCELLILDRSIDQIAPLIHEWTYDAMCHDLLNMEGNKYTHEVPSKT 300

Query: 301 GGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEIS 360
           G +P+K+E +L++ D +W+ELR  HIADASERLHEKMT F +KNKAAQ++ +++D G++S
Sbjct: 301 GDKPEKKEVLLDEEDSIWVELRDAHIADASERLHEKMTNFVSKNKAAQLKHSSKDFGDLS 360

Query: 361 TRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKEV 420
           ++DLQKMV ALPQY+EQ++K++LHVEIA  IN+ I E GLR+LGQLEQDLVFGDAG K+V
Sbjct: 361 SKDLQKMVHALPQYSEQIDKLSLHVEIARTINRTIMEQGLRDLGQLEQDLVFGDAGRKDV 420

Query: 421 INFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLG- 480
           I FL T    S E+KLRL+MI A++YP+KFE +K  K+M+LAKLS +D+  V NM+LLG 
Sbjct: 421 IKFLSTNHIISHESKLRLIMIVAAIYPKKFEGEKGRKMMELAKLSGDDVVAVNNMRLLGP 480

Query: 481 -GSDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELP 540
             ++ KK+T+  SF LKF   KTK+AAR+DR GE +TWQL RFYP++EELVEKL KG LP
Sbjct: 481 VHTECKKSTTG-SFPLKFDVLKTKRAARRDRVGETQTWQLSRFYPIVEELVEKLSKGHLP 540

Query: 541 KSEYSCMNEPPPSTEKPATKGTRITASQTSQSTGGPKSMRSRRTANWARTSVSDDGYGSD 600
           K +Y CMNEP P+             S +   +  P    SRRT  WAR  +SDDGY SD
Sbjct: 541 KQDYPCMNEPKPT-----------FYSGSLSPSASPVLPHSRRTPTWARRHLSDDGYFSD 600

Query: 601 SVLRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITK 660
           SVL   +   K+ G+R+FVF++GGATRSELRVCHKLT KL REV+LG SS  DP  ++TK
Sbjct: 601 SVLGRASSGFKRKGQRIFVFIVGGATRSELRVCHKLTEKLDREVILGSSSFLDPLTFLTK 653

Query: 661 LKQLSEKD 664
           +KQL+E++
Sbjct: 661 MKQLNEEE 653

BLAST of Sed0012324 vs. ExPASy TrEMBL
Match: A0A1S3CD33 (protein transport Sec1a-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499028 PE=3 SV=1)

HSP 1 Score: 1171.8 bits (3030), Expect = 0.0e+00
Identity = 585/675 (86.67%), Postives = 630/675 (93.33%), Query Frame = 0

Query: 1   MSFSDSD---VGG-NEYKNFRQTTRDRLLFEMLGAANTENSKPWKVLIMDRVTVKVMSHS 60
           MSFSDSD   +GG NEYKNFRQT+RDRLL+EMLGAANTENSKPWKVLIMD+VTVKVMSHS
Sbjct: 1   MSFSDSDSCSIGGHNEYKNFRQTSRDRLLYEMLGAANTENSKPWKVLIMDKVTVKVMSHS 60

Query: 61  CKMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVF 120
           CKMADITDQG+SLVEDLFRRRQPLPSMDAIYF+QPSKENVVMFLSDMSGREPLYKKAFVF
Sbjct: 61  CKMADITDQGVSLVEDLFRRRQPLPSMDAIYFIQPSKENVVMFLSDMSGREPLYKKAFVF 120

Query: 121 FSSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFIGTENSR 180
           FSSPVPKEF+NHIKCDTSVLPRIGALREMNLEYFPIDSQAF TDQE AL DLF   ENSR
Sbjct: 121 FSSPVPKEFVNHIKCDTSVLPRIGALREMNLEYFPIDSQAFFTDQERALEDLFGDIENSR 180

Query: 181 KFDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNC 240
           KFDNCLNTMATRIATVFASLKEFPFVRY+ SKAL   DD TEAS REL+P+KLAAAIWNC
Sbjct: 181 KFDNCLNTMATRIATVFASLKEFPFVRYRASKAL---DDPTEASLRELVPTKLAAAIWNC 240

Query: 241 VSKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSK 300
           +SKYK+TIPNYPQ+ETCELLILDRSIDQIAP+IHEWTYDAMCRDLLEM+GNKY YEVSSK
Sbjct: 241 ISKYKTTIPNYPQSETCELLILDRSIDQIAPVIHEWTYDAMCRDLLEMDGNKYTYEVSSK 300

Query: 301 TGGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEI 360
           TGG PDKREA+LEDTDPVWLELRH+HIADASERLHEKMT F +KNKAAQIQQ+ARDGGEI
Sbjct: 301 TGGAPDKREALLEDTDPVWLELRHSHIADASERLHEKMTNFVSKNKAAQIQQSARDGGEI 360

Query: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKE 420
           STRDLQKMVQALPQYTEQVEKITLHVEIAGK+NKLIRE+GLR+LGQLEQDLVFGDAGAK+
Sbjct: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKVNKLIRELGLRDLGQLEQDLVFGDAGAKD 420

Query: 421 VINFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLG 480
           VIN+LRT Q+ S ENKLRLLMIYASVYPEKFE+DKA+KIMQLAKLSTEDMK+VKNM+LL 
Sbjct: 421 VINYLRTNQNASPENKLRLLMIYASVYPEKFEDDKALKIMQLAKLSTEDMKVVKNMRLLA 480

Query: 481 GSDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELPK 540
           GSDSKK +S+HSFSLKF+ QKTKQA RKDRTGEEETWQLFRFYPMIEEL+E LCKG+L K
Sbjct: 481 GSDSKKTSSAHSFSLKFNAQKTKQATRKDRTGEEETWQLFRFYPMIEELIENLCKGDLSK 540

Query: 541 SEYSCMNEPPPSTEKPATKGTRITASQTSQSTGGPKSMRSRRTANWARTSVSDDGYGSDS 600
           SEYSCMNEPPP TEKPA KG++   SQT QSTGGPKSMRSRRTANWAR+S+SDDGYGSDS
Sbjct: 541 SEYSCMNEPPPVTEKPAPKGSQSATSQTGQSTGGPKSMRSRRTANWARSSISDDGYGSDS 600

Query: 601 VLRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKL 660
           +LR    D K+MG+RVFVF+IGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKL
Sbjct: 601 ILRAATLDFKKMGQRVFVFIIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKL 660

Query: 661 KQLSEKDLQTEPPRF 672
           K L+E+ +  E PRF
Sbjct: 661 KLLTERGISVEAPRF 672

BLAST of Sed0012324 vs. ExPASy TrEMBL
Match: A0A0A0KEN9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G077380 PE=3 SV=1)

HSP 1 Score: 1167.9 bits (3020), Expect = 0.0e+00
Identity = 582/675 (86.22%), Postives = 629/675 (93.19%), Query Frame = 0

Query: 1   MSFSDSD---VGG-NEYKNFRQTTRDRLLFEMLGAANTENSKPWKVLIMDRVTVKVMSHS 60
           MSFSDSD   +GG NEYKNFRQT+RDRLL+EMLGAANTENSKPWKVLIMD+VTVKVMSHS
Sbjct: 1   MSFSDSDSSSIGGHNEYKNFRQTSRDRLLYEMLGAANTENSKPWKVLIMDKVTVKVMSHS 60

Query: 61  CKMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVF 120
           CKMADITDQG+SLVEDLFRRRQPLPSMDAIYF+QPSKENVVMFLSDMSGREPLYKKAFVF
Sbjct: 61  CKMADITDQGVSLVEDLFRRRQPLPSMDAIYFIQPSKENVVMFLSDMSGREPLYKKAFVF 120

Query: 121 FSSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFIGTENSR 180
           FSSPVPKEF+NHIKCDTSVLPRIGALREMNLEYFPIDSQAF TDQE AL DLF   ENSR
Sbjct: 121 FSSPVPKEFVNHIKCDTSVLPRIGALREMNLEYFPIDSQAFFTDQERALEDLFGDIENSR 180

Query: 181 KFDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNC 240
           KFDNCLNTMATRIATVFASLKEFPFV+Y+ SKAL   DD TEAS REL+P+KLAAAIWNC
Sbjct: 181 KFDNCLNTMATRIATVFASLKEFPFVKYRASKAL---DDPTEASLRELVPTKLAAAIWNC 240

Query: 241 VSKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSK 300
           +SKYK+TIPNYPQ+ETCELLILDRSIDQIAP+IHEWTYDAMCRDLLEM+GNKY YEVSSK
Sbjct: 241 ISKYKTTIPNYPQSETCELLILDRSIDQIAPVIHEWTYDAMCRDLLEMDGNKYTYEVSSK 300

Query: 301 TGGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEI 360
           TGG PD+REA+LEDTDPVWLELRH+HIADASERLHEKMT F +KNKAAQIQQNARDGGE+
Sbjct: 301 TGGAPDRREALLEDTDPVWLELRHSHIADASERLHEKMTNFVSKNKAAQIQQNARDGGEV 360

Query: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKE 420
           STRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIRE+GLR+LGQLEQDLVFGDAGAK+
Sbjct: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIRELGLRDLGQLEQDLVFGDAGAKD 420

Query: 421 VINFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLG 480
           VIN+LRT Q+ S ENKLRLLMIYASVYPEKFE+DKA+KIMQLAKLSTEDMK+VKNM+LLG
Sbjct: 421 VINYLRTNQNASPENKLRLLMIYASVYPEKFEDDKALKIMQLAKLSTEDMKVVKNMRLLG 480

Query: 481 GSDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELPK 540
           GSDSKKA+S HSFSLKF+ QKTKQA RKDRTGEEETWQLFRFYPMIEEL+E LCKG+L K
Sbjct: 481 GSDSKKASSGHSFSLKFNAQKTKQATRKDRTGEEETWQLFRFYPMIEELIENLCKGDLSK 540

Query: 541 SEYSCMNEPPPSTEKPATKGTRITASQTSQSTGGPKSMRSRRTANWARTSVSDDGYGSDS 600
           SEYSC+NEPPP TEK   KG++   SQT QSTGGPKSMRSRRTANWAR+S+SDDGYGSDS
Sbjct: 541 SEYSCINEPPPVTEKAPPKGSQSATSQTGQSTGGPKSMRSRRTANWARSSISDDGYGSDS 600

Query: 601 VLRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKL 660
           +L+    D K+MG+RVFVF++GGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKL
Sbjct: 601 ILKAATLDFKKMGQRVFVFIVGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKL 660

Query: 661 KQLSEKDLQTEPPRF 672
           K L+EK +  E PRF
Sbjct: 661 KLLTEKGISVEAPRF 672

BLAST of Sed0012324 vs. ExPASy TrEMBL
Match: A0A6J1KJ54 (protein transport Sec1a-like OS=Cucurbita maxima OX=3661 GN=LOC111494459 PE=3 SV=1)

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 578/678 (85.25%), Postives = 625/678 (92.18%), Query Frame = 0

Query: 1   MSFSDSD---VGG-NEYKNFRQTTRDRLLFEMLGAANTENSKPWKVLIMDRVTVKVMSHS 60
           MSFSDSD   VGG NEYKNFRQT+RDRLLFEMLGAANTENSKPW+VLIMD+VTVKVMSHS
Sbjct: 1   MSFSDSDSSSVGGTNEYKNFRQTSRDRLLFEMLGAANTENSKPWRVLIMDKVTVKVMSHS 60

Query: 61  CKMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVF 120
           CKMADITDQG+SLVEDLFRRRQPLPS+DAIYF+QPSKENVVMFLSDMSGREPLYKKAFVF
Sbjct: 61  CKMADITDQGVSLVEDLFRRRQPLPSLDAIYFIQPSKENVVMFLSDMSGREPLYKKAFVF 120

Query: 121 FSSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFIGTENSR 180
           FSSPVP+EF+NHIKCDTSVLPRIGALREMNLEYFPIDSQAFITD + AL DLF+ +ENSR
Sbjct: 121 FSSPVPREFVNHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDHDRALDDLFVESENSR 180

Query: 181 KFDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNC 240
           +FDNCLNTMATRIATVFASLKEFPFVRY+ SKAL   +D T AS REL+P+KLAAA+WNC
Sbjct: 181 RFDNCLNTMATRIATVFASLKEFPFVRYRASKAL---EDPTAASLRELVPTKLAAAVWNC 240

Query: 241 VSKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSK 300
           +SKYKSTIPNYPQ+ETCELLILDRSIDQIAP+IHEWTYDAMCRDLLEMEGNKY+YEVSSK
Sbjct: 241 ISKYKSTIPNYPQSETCELLILDRSIDQIAPVIHEWTYDAMCRDLLEMEGNKYIYEVSSK 300

Query: 301 TGGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEI 360
           TGG+PDKREA+LEDTDPVWLELRH HIADASERLHEKMTTF +KNKAAQI Q ARDGGEI
Sbjct: 301 TGGDPDKREALLEDTDPVWLELRHAHIADASERLHEKMTTFASKNKAAQIHQTARDGGEI 360

Query: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKE 420
           STRDLQKMVQALPQYTEQVEKITLHVEIAGKIN LIREMGLRELGQLEQDLVFGDAGAK+
Sbjct: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINTLIREMGLRELGQLEQDLVFGDAGAKD 420

Query: 421 VINFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLG 480
           VINFLRT Q+ S ENKLRLLMIYASVYPEKFE+DKA KIMQLAKL+ EDM +VKNM+LL 
Sbjct: 421 VINFLRTNQNASPENKLRLLMIYASVYPEKFEDDKAAKIMQLAKLTAEDMTVVKNMRLLA 480

Query: 481 GSDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELPK 540
           GSD+KKATS+HSFSLKF+ QKTKQA RKDRTGEEETWQLFRFYPMIEEL+E LCKGEL K
Sbjct: 481 GSDAKKATSAHSFSLKFNAQKTKQATRKDRTGEEETWQLFRFYPMIEELIENLCKGELSK 540

Query: 541 SEYSCMNEPPPSTEKPATK--GTRITASQTSQSTGGPKSMRSRRTANWARTSVSDDGYGS 600
           +EYSCMNEP PST KPA     T+   SQTSQS GGPKSMRSRRTANWAR+S+SDDGYGS
Sbjct: 541 NEYSCMNEPSPSTTKPAVPRGSTQSATSQTSQSAGGPKSMRSRRTANWARSSISDDGYGS 600

Query: 601 DSVLRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYIT 660
           DS+L+    D K+MG+RVFVF+IGGATRSELRVCHKLTAKLRREVVLGCS+LDDPPQYI 
Sbjct: 601 DSILKAATLDFKKMGQRVFVFIIGGATRSELRVCHKLTAKLRREVVLGCSTLDDPPQYIM 660

Query: 661 KLKQLSEKDLQTEPPRFF 673
           KLK+LSE +   E P+FF
Sbjct: 661 KLKRLSEPEPPVEAPKFF 675

BLAST of Sed0012324 vs. ExPASy TrEMBL
Match: A0A6J1HM40 (protein transport Sec1a-like OS=Cucurbita moschata OX=3662 GN=LOC111464227 PE=3 SV=1)

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 578/678 (85.25%), Postives = 625/678 (92.18%), Query Frame = 0

Query: 1   MSFSDSD---VGG-NEYKNFRQTTRDRLLFEMLGAANTENSKPWKVLIMDRVTVKVMSHS 60
           MSFSDSD   VGG NEYKNFRQT+RDRLLFEMLGAANTENSKPW+VLIMD+VTVKVMSHS
Sbjct: 1   MSFSDSDSSSVGGTNEYKNFRQTSRDRLLFEMLGAANTENSKPWRVLIMDKVTVKVMSHS 60

Query: 61  CKMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVF 120
           CKMADITDQG+SLVEDLFRRRQPLPS+DAIYF+QPSKENVVMFLSDMSGREPLYKKAFVF
Sbjct: 61  CKMADITDQGVSLVEDLFRRRQPLPSLDAIYFIQPSKENVVMFLSDMSGREPLYKKAFVF 120

Query: 121 FSSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFIGTENSR 180
           FSSPVP+EF+NHIKCDTSVLPRIGALREMNLEYFPIDSQAFITD + AL DLF+ +ENSR
Sbjct: 121 FSSPVPREFVNHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDHDRALDDLFVESENSR 180

Query: 181 KFDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNC 240
           +FDNCLNTMATRIATVFASLKEFPFVRY+ SKAL   +D T AS REL+P+KLAAA+WNC
Sbjct: 181 RFDNCLNTMATRIATVFASLKEFPFVRYRASKAL---EDPTAASLRELVPTKLAAAVWNC 240

Query: 241 VSKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSK 300
           +SKYKSTIPNYPQ+ETCELLILDRSIDQIAP+IHEWTYDAMCRDLLEMEGNKY+YEVSSK
Sbjct: 241 ISKYKSTIPNYPQSETCELLILDRSIDQIAPVIHEWTYDAMCRDLLEMEGNKYIYEVSSK 300

Query: 301 TGGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEI 360
           TGG+PDKREA+LEDTDPVWLELRH HIADASERLHEKMTTF +KN+AAQI Q ARDGGEI
Sbjct: 301 TGGDPDKREALLEDTDPVWLELRHAHIADASERLHEKMTTFASKNRAAQIHQTARDGGEI 360

Query: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKE 420
           STRDLQKMVQALPQYTEQVEKITLHVEIAGKIN LIREMGLRELGQLEQDLVFGDAGAK+
Sbjct: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINTLIREMGLRELGQLEQDLVFGDAGAKD 420

Query: 421 VINFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLG 480
           VINFLRT Q+ S ENKLRLLMIYASVYPEKFE+DKA KIMQLAKL+ EDM +VKNM+LL 
Sbjct: 421 VINFLRTNQNASPENKLRLLMIYASVYPEKFEDDKAAKIMQLAKLTAEDMTVVKNMRLLA 480

Query: 481 GSDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELPK 540
           GSDSKKATS+HSFSLKF+ QKTKQA RKDRTGEEETWQLFRFYPMIEEL+E LCKGEL K
Sbjct: 481 GSDSKKATSAHSFSLKFNAQKTKQATRKDRTGEEETWQLFRFYPMIEELIENLCKGELSK 540

Query: 541 SEYSCMNEPPPSTEKPATK--GTRITASQTSQSTGGPKSMRSRRTANWARTSVSDDGYGS 600
           +EYSCMNEP PST KPA     T+   SQTSQS GGPKSMRSRRTANWAR+S+SDDGYGS
Sbjct: 541 NEYSCMNEPSPSTTKPAVPRGSTQSATSQTSQSAGGPKSMRSRRTANWARSSISDDGYGS 600

Query: 601 DSVLRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYIT 660
           DS+L+    D K+MG+RVFVF+IGGATRSELRVCHKLTAKLRREVVLGCS+LDDPPQYI 
Sbjct: 601 DSILKAATLDFKKMGQRVFVFIIGGATRSELRVCHKLTAKLRREVVLGCSTLDDPPQYIM 660

Query: 661 KLKQLSEKDLQTEPPRFF 673
           KLK+LSE +   E P+FF
Sbjct: 661 KLKRLSEPEPPVEAPKFF 675

BLAST of Sed0012324 vs. ExPASy TrEMBL
Match: A0A6J1CVL6 (protein transport Sec1a-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111014784 PE=3 SV=1)

HSP 1 Score: 1125.5 bits (2910), Expect = 0.0e+00
Identity = 567/673 (84.25%), Postives = 622/673 (92.42%), Query Frame = 0

Query: 1   MSFSDSD---VGG-NEYKNFRQTTRDRLLFEMLGAANTENSKPWKVLIMDRVTVKVMSHS 60
           MSFSDSD   VGG NEYKNFRQ TRDRLLFEMLGAAN+ +SKPWKVLIMD+VTVKVMSHS
Sbjct: 1   MSFSDSDSSSVGGANEYKNFRQATRDRLLFEMLGAANSGDSKPWKVLIMDKVTVKVMSHS 60

Query: 61  CKMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVF 120
           CKMADITDQG+SLVEDLFRRRQPLPSMDAIYFVQPSKEN+VMFLSDMSGREPLYKKAFVF
Sbjct: 61  CKMADITDQGVSLVEDLFRRRQPLPSMDAIYFVQPSKENIVMFLSDMSGREPLYKKAFVF 120

Query: 121 FSSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFIGTENSR 180
           FSSPVPKEFINHIK DTSVLPRIGALREMNLEYFPIDSQAFITDQE AL D+F   EN+R
Sbjct: 121 FSSPVPKEFINHIKGDTSVLPRIGALREMNLEYFPIDSQAFITDQERALEDIFGDVENTR 180

Query: 181 KFDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNC 240
           +FD+CLNTMATRIATVFASLKEFPFVRY+ SKAL   DD T AS REL+P+KLAAAIWNC
Sbjct: 181 RFDSCLNTMATRIATVFASLKEFPFVRYRASKAL---DDPTAASNRELVPTKLAAAIWNC 240

Query: 241 VSKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSK 300
           +SKYK+TIPNYPQ+ETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEM+GNKY YEVSSK
Sbjct: 241 ISKYKTTIPNYPQSETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMDGNKYTYEVSSK 300

Query: 301 TGGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEI 360
           TGGEP+KREA+LED DPVWLELRH HIADASERLHEKMT F +KNKAAQ+Q++ARD GEI
Sbjct: 301 TGGEPEKREALLEDNDPVWLELRHAHIADASERLHEKMTNFVSKNKAAQLQKSARD-GEI 360

Query: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKE 420
           STRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAK+
Sbjct: 361 STRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKD 420

Query: 421 VINFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLG 480
           VINFLRT Q+ S ENKLRLLM+YASVYPEKFE+D+A+KIMQLAKLS +DMK++KNM+LLG
Sbjct: 421 VINFLRTNQNSSPENKLRLLMVYASVYPEKFEDDRAIKIMQLAKLSADDMKVIKNMRLLG 480

Query: 481 GSDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELPK 540
           GS+SKKA+S+  FSLKF+ QKTKQAARKDRTGEEETWQLFRFYPM+EEL+EKL KGE+ K
Sbjct: 481 GSESKKASSTDGFSLKFNAQKTKQAARKDRTGEEETWQLFRFYPMLEELIEKLSKGEISK 540

Query: 541 SEYSCMNEPPPSTEKPATKGTRITASQTSQSTGGPKSMRSRRTANWARTSVSDDGYGSDS 600
           S YSCMNEPPPSTEK + KGT    SQT+ STGGPKSMRSRRTANWAR+S+SDDGYGSDS
Sbjct: 541 SGYSCMNEPPPSTEKASQKGTHSATSQTT-STGGPKSMRSRRTANWARSSISDDGYGSDS 600

Query: 601 VLRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKL 660
           VL+    D K+MG+RVF+F++GGATRSELRVCHKLTAK+RRE+VLGCSSLDDPPQYITK+
Sbjct: 601 VLKAATIDFKKMGQRVFIFIVGGATRSELRVCHKLTAKIRREIVLGCSSLDDPPQYITKM 660

Query: 661 KQLSEKDLQTEPP 670
           K+L+E+D   E P
Sbjct: 661 KRLTERDETVEVP 668

BLAST of Sed0012324 vs. TAIR 10
Match: AT1G02010.1 (secretory 1A )

HSP 1 Score: 931.8 bits (2407), Expect = 3.1e-271
Identity = 478/681 (70.19%), Postives = 561/681 (82.38%), Query Frame = 0

Query: 1   MSFSDSDV-----GGNEYKNFRQTTRDRLLFEMLGAANTENSKPWKVLIMDRVTVKVMSH 60
           MSFSDS+      GG +YK FRQ +RDRLL EMLG+  T +SK WK+LIMDRVTVKVMS 
Sbjct: 1   MSFSDSESSSHGGGGGDYKFFRQISRDRLLHEMLGSTKTGDSKAWKILIMDRVTVKVMSQ 60

Query: 61  SCKMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFV 120
           SCKMADITDQGISLVE+LF+RR+P+P MDAIYF+QPSKEN+VMFLSDMSGREPLY+KAF+
Sbjct: 61  SCKMADITDQGISLVEELFKRREPMPGMDAIYFIQPSKENIVMFLSDMSGREPLYRKAFI 120

Query: 121 FFSSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFI-GTEN 180
           FFSS +PKE +NHIK D+SVLPRIGALREMN+EYFPID+Q F+TD E AL  L+    EN
Sbjct: 121 FFSSTIPKELVNHIKSDSSVLPRIGALREMNMEYFPIDNQGFLTDHEQALETLYAEDAEN 180

Query: 181 SRKFDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIW 240
           SR F  CLN MATRIATVFASLKE PFVRY+ +K+          + R+L+PSKLAAAIW
Sbjct: 181 SRHFHICLNIMATRIATVFASLKELPFVRYRAAKS---------TASRDLVPSKLAAAIW 240

Query: 241 NCVSKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVS 300
           +C+SKYK+ IPN+PQ ETCELLI+DRS+DQIAPIIHEWTYDAMC DLL+MEGNK+V EV 
Sbjct: 241 DCISKYKA-IPNFPQTETCELLIVDRSVDQIAPIIHEWTYDAMCHDLLDMEGNKHVIEVP 300

Query: 301 SKTGGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGG 360
           SKTGG P+K+E +LED DPVWLELRHTHIADASERLHEKMT F +KNKAAQ++  +RDG 
Sbjct: 301 SKTGGPPEKKEIVLEDHDPVWLELRHTHIADASERLHEKMTNFASKNKAAQMR--SRDGS 360

Query: 361 EISTRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGA 420
           E+STRDLQK+VQALPQY EQV+K++ HVE+AGKIN++IR+ GLR+LGQLEQDLVFGDAGA
Sbjct: 361 ELSTRDLQKIVQALPQYGEQVDKLSTHVELAGKINRIIRDTGLRDLGQLEQDLVFGDAGA 420

Query: 421 KEVINFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQL 480
           K+VINFLRT Q  + ENKLRLLMIYA+VYPEKFE DK VK+MQLA+LS  DMK++ NMQL
Sbjct: 421 KDVINFLRTNQDTNPENKLRLLMIYATVYPEKFEGDKGVKLMQLARLSPVDMKVISNMQL 480

Query: 481 LGGSDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGEL 540
           + GS   KA S  SFSLKF   KTKQA RKDR+GEEETWQLFRFYPMIEEL+EKL KG+L
Sbjct: 481 IAGSPENKAKSG-SFSLKFDAGKTKQANRKDRSGEEETWQLFRFYPMIEELLEKLVKGDL 540

Query: 541 PKSEYSCMNEPPPSTEKPATKGT-RITASQTS--QSTGGPKSMRSRRTANWARTSVSDDG 600
            KS+Y CMN+     E  A  G+ R +++ T+  +    P SMRSRRTA WAR   SDDG
Sbjct: 541 SKSDYLCMNQSSHKEESEARTGSVRKSSAPTAVPERKATPHSMRSRRTATWARPHSSDDG 600

Query: 601 YGSDSVLRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQ 660
           Y SDSVL+  + + K++G+R+FVF+IGGATRSELRVCHKLT+ LRREVVLG +S DDPPQ
Sbjct: 601 YSSDSVLKSASTEFKKLGQRIFVFIIGGATRSELRVCHKLTSSLRREVVLGSTSFDDPPQ 660

Query: 661 YITKLKQLSEKDLQTEPPRFF 673
           YITKLK LSEKD+Q  P + F
Sbjct: 661 YITKLKLLSEKDIQGAPAQPF 668

BLAST of Sed0012324 vs. TAIR 10
Match: AT1G02010.2 (secretory 1A )

HSP 1 Score: 904.8 bits (2337), Expect = 4.1e-263
Identity = 468/681 (68.72%), Postives = 552/681 (81.06%), Query Frame = 0

Query: 1   MSFSDSDV-----GGNEYKNFRQTTRDRLLFEMLGAANTENSKPWKVLIMDRVTVKVMSH 60
           MSFSDS+      GG +YK FRQ +RDRLL EMLG+  T +SK WK+LIMDRVTVKVMS 
Sbjct: 1   MSFSDSESSSHGGGGGDYKFFRQISRDRLLHEMLGSTKTGDSKAWKILIMDRVTVKVMSQ 60

Query: 61  SCKMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFV 120
           SCKMADITDQGISLVE+LF+RR+P+P MDAIYF+QPSKEN+VMFLSDMSGREPLY+KAF+
Sbjct: 61  SCKMADITDQGISLVEELFKRREPMPGMDAIYFIQPSKENIVMFLSDMSGREPLYRKAFI 120

Query: 121 FFSSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFI-GTEN 180
           FFSS +PKE +NHIK D+SVLPRIGALREMN+EYFPID+Q F+TD E AL  L+    EN
Sbjct: 121 FFSSTIPKELVNHIKSDSSVLPRIGALREMNMEYFPIDNQGFLTDHEQALETLYAEDAEN 180

Query: 181 SRKFDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIW 240
           SR F  CLN MATRIATVFASLKE PFVRY+ +K+          + R+L+PSKLAAAIW
Sbjct: 181 SRHFHICLNIMATRIATVFASLKELPFVRYRAAKS---------TASRDLVPSKLAAAIW 240

Query: 241 NCVSKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVS 300
           +C+SKYK+ IPN+PQ ETCELLI+DRS+DQIAPIIHEWTYDAMC DLL+MEGNK+V EV 
Sbjct: 241 DCISKYKA-IPNFPQTETCELLIVDRSVDQIAPIIHEWTYDAMCHDLLDMEGNKHVIEVP 300

Query: 301 SKTGGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGG 360
           SKTGG P+K+E +LED DPVWLELRHTHIADASERLHEKMT F +KNKAAQ++  +RDG 
Sbjct: 301 SKTGGPPEKKEIVLEDHDPVWLELRHTHIADASERLHEKMTNFASKNKAAQMR--SRDGS 360

Query: 361 EISTRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGA 420
           E+STRDLQK+VQALPQY EQV+K++ HVE+AGKIN++IR+ GLR+LGQLEQDLVFGDAGA
Sbjct: 361 ELSTRDLQKIVQALPQYGEQVDKLSTHVELAGKINRIIRDTGLRDLGQLEQDLVFGDAGA 420

Query: 421 KEVINFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQL 480
           K+VINFLRT Q  + ENKLRLLMIYA+VYPEKFE DK VK+MQLA+LS  DMK++ NMQL
Sbjct: 421 KDVINFLRTNQDTNPENKLRLLMIYATVYPEKFEGDKGVKLMQLARLSPVDMKVISNMQL 480

Query: 481 LGGSDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGEL 540
           + GS   KA S  SFSLKF   KTKQA RKDR+GEEETWQLFRFYPMIEEL+EKL KG+L
Sbjct: 481 IAGSPENKAKSG-SFSLKFDAGKTKQANRKDRSGEEETWQLFRFYPMIEELLEKLVKGDL 540

Query: 541 PKSEYSCMNEPPPSTEKPATKGT-RITASQTS--QSTGGPKSMRSRRTANWARTSVSDDG 600
            KS+Y CMN+     E  A  G+ R +++ T+  +    P SMRSRRTA WA        
Sbjct: 541 SKSDYLCMNQSSHKEESEARTGSVRKSSAPTAVPERKATPHSMRSRRTATWA-------- 600

Query: 601 YGSDSVLRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQ 660
                +L+  + + K++G+R+FVF+IGGATRSELRVCHKLT+ LRREVVLG +S DDPPQ
Sbjct: 601 -----LLKSASTEFKKLGQRIFVFIIGGATRSELRVCHKLTSSLRREVVLGSTSFDDPPQ 655

Query: 661 YITKLKQLSEKDLQTEPPRFF 673
           YITKLK LSEKD+Q  P + F
Sbjct: 661 YITKLKLLSEKDIQGAPAQPF 655

BLAST of Sed0012324 vs. TAIR 10
Match: AT1G12360.1 (Sec1/munc18-like (SM) proteins superfamily )

HSP 1 Score: 880.9 bits (2275), Expect = 6.3e-256
Identity = 443/666 (66.52%), Postives = 537/666 (80.63%), Query Frame = 0

Query: 1   MSFSDSDVG--GNEYKNFRQTTRDRLLFEMLGAANTENSK-PWKVLIMDRVTVKVMSHSC 60
           MS+SDSD    G EYKNFRQ TR+RLL+EML +A T +SK  WKVLIMD++TVK+MS++C
Sbjct: 1   MSYSDSDSSSHGGEYKNFRQITRERLLYEMLRSAKTGSSKSTWKVLIMDKLTVKIMSYAC 60

Query: 61  KMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVFF 120
           KMADIT +G+SLVED+FRRRQPLPSMDAIYF+QP+KENV+MFLSDMSG+ PLYKKAFVFF
Sbjct: 61  KMADITQEGVSLVEDIFRRRQPLPSMDAIYFIQPTKENVIMFLSDMSGKSPLYKKAFVFF 120

Query: 121 SSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFIGTENSRK 180
           SSPV KE + HIK D+SVLPRIGALREMNLE+F IDSQ FITD E AL DLF   E SRK
Sbjct: 121 SSPVSKELVGHIKKDSSVLPRIGALREMNLEFFAIDSQGFITDHERALEDLFGDEETSRK 180

Query: 181 FDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNCV 240
            D CLN MA+RIATVFASL+EFP VRY+ +K+LD     T  + R+LIP+KLAA IWNC+
Sbjct: 181 GDACLNVMASRIATVFASLREFPAVRYRAAKSLDAS---TMTTLRDLIPTKLAAGIWNCL 240

Query: 241 SKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSKT 300
           +K+K +I N+PQ ETCELLILDRSIDQIAP+IHEWTYDAMC DLL MEGNKYV+ + SK+
Sbjct: 241 AKHKQSIENFPQTETCELLILDRSIDQIAPVIHEWTYDAMCHDLLNMEGNKYVHVIPSKS 300

Query: 301 GGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEIS 360
           GG+P+K++ +LE+ DP+WLELRH HIADASERLH+KMT F +KNKAAQ+ Q  RDG E+S
Sbjct: 301 GGQPEKKDVLLEEHDPIWLELRHAHIADASERLHDKMTNFLSKNKAAQL-QGKRDGAELS 360

Query: 361 TRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKEV 420
           TRDLQKMVQALPQY+EQ++K++LHVEIA K+N LIRE GLRELGQLEQDLVFGDAG K+V
Sbjct: 361 TRDLQKMVQALPQYSEQIDKLSLHVEIARKLNDLIREQGLRELGQLEQDLVFGDAGMKDV 420

Query: 421 INFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLGG 480
           I +L T +  S E KLRLLMI A++YPEKFE +K   +M+LAKLS++DM  V NM LLG 
Sbjct: 421 IKYLSTQEEASREGKLRLLMILATIYPEKFEGEKGQNLMKLAKLSSDDMTAVNNMSLLGS 480

Query: 481 SDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELPKS 540
           +   K  +   F+LKF   K K+A RK+R  EE  WQL RFYPMIEEL+EKL KGELPK 
Sbjct: 481 AVDAKKNTPGGFTLKFDLHKKKRAVRKERQ-EEAAWQLSRFYPMIEELIEKLSKGELPKE 540

Query: 541 EYSCMNEPPPSTEKPATKGTRITASQTSQSTGGPKSMRSRRTANWARTSVSDDGYGSDSV 600
           ++ CMN+P PS        T ++++ +S      +SMRSRRT  WA+   SDDGY SDSV
Sbjct: 541 DFPCMNDPSPSFH----GSTSLSSAASSSQGQAAQSMRSRRTPTWAKPRGSDDGYSSDSV 600

Query: 601 LRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITKLK 660
           LRH + D ++MG+R+FVF++GGATRSEL+VCHKL+ KL+REV+LG +SLDDPPQ+ITKLK
Sbjct: 601 LRHASSDFRKMGQRIFVFIVGGATRSELKVCHKLSTKLKREVILGSTSLDDPPQFITKLK 657

Query: 661 QLSEKD 664
            L+  D
Sbjct: 661 LLTAND 657

BLAST of Sed0012324 vs. TAIR 10
Match: AT4G12120.1 (Sec1/munc18-like (SM) proteins superfamily )

HSP 1 Score: 781.2 bits (2016), Expect = 6.8e-226
Identity = 399/668 (59.73%), Postives = 508/668 (76.05%), Query Frame = 0

Query: 1   MSFSDSDVG--GNEYKNFRQTTRDRLLFEMLGAANTENSK-PWKVLIMDRVTVKVMSHSC 60
           MSFSDS     G EYKNFRQ TR+RLL EML      +SK  WKVL+MD+ TVK+MS +C
Sbjct: 1   MSFSDSGSSSYGGEYKNFRQITRERLLCEMLRPERNGSSKLTWKVLVMDKFTVKIMSSAC 60

Query: 61  KMADITDQGISLVEDLFRRRQPLPSMDAIYFVQPSKENVVMFLSDMSGREPLYKKAFVFF 120
           KM++IT +GISLVE + + RQP+ +M+ IYF+QP++ENV  FLSDM+G+ PLYKKAFVFF
Sbjct: 61  KMSEITQEGISLVEVITKHRQPMTAMEVIYFIQPTEENVTAFLSDMTGKSPLYKKAFVFF 120

Query: 121 SSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAFITDQEMALHDLFIGTENSRK 180
           SSPV +  +N IK D   + RIG L+EMNLEY  +D Q F+T+ E AL +LF   EN ++
Sbjct: 121 SSPVSRSLVNLIKKDMRAMKRIGGLKEMNLEYISMDIQGFVTNNENALEELFCDDENHQR 180

Query: 181 FDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDDLTEASFRELIPSKLAAAIWNCV 240
            D CLN +A RIATV ASLKE+PFVRY+ +KAL   D  T  ++RELIP+KLAA++WNC+
Sbjct: 181 ADACLNVVAKRIATVLASLKEYPFVRYRGAKAL---DATTMTTYRELIPTKLAASVWNCL 240

Query: 241 SKYKSTIPNYPQAETCELLILDRSIDQIAPIIHEWTYDAMCRDLLEMEGNKYVYEVSSKT 300
           ++YK TI ++PQ ETCELLILDRSIDQIAP+IHEWTYDAMC DLL MEGNKY +EV SKT
Sbjct: 241 ARYKQTIEDFPQTETCELLILDRSIDQIAPLIHEWTYDAMCHDLLNMEGNKYTHEVPSKT 300

Query: 301 GGEPDKREAILEDTDPVWLELRHTHIADASERLHEKMTTFTAKNKAAQIQQNARDGGEIS 360
           G +P+K+E +L++ D +W+ELR  HIADASERLHEKMT F +KNKAAQ++ +++D G++S
Sbjct: 301 GDKPEKKEVLLDEEDSIWVELRDAHIADASERLHEKMTNFVSKNKAAQLKHSSKDFGDLS 360

Query: 361 TRDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIREMGLRELGQLEQDLVFGDAGAKEV 420
           ++DLQKMV ALPQY+EQ++K++LHVEIA  IN+ I E GLR+LGQLEQDLVFGDAG K+V
Sbjct: 361 SKDLQKMVHALPQYSEQIDKLSLHVEIARTINRTIMEQGLRDLGQLEQDLVFGDAGRKDV 420

Query: 421 INFLRTYQSGSSENKLRLLMIYASVYPEKFENDKAVKIMQLAKLSTEDMKIVKNMQLLG- 480
           I FL T    S E+KLRL+MI A++YP+KFE +K  K+M+LAKLS +D+  V NM+LLG 
Sbjct: 421 IKFLSTNHIISHESKLRLIMIVAAIYPKKFEGEKGRKMMELAKLSGDDVVAVNNMRLLGP 480

Query: 481 -GSDSKKATSSHSFSLKFSNQKTKQAARKDRTGEEETWQLFRFYPMIEELVEKLCKGELP 540
             ++ KK+T+  SF LKF   KTK+AAR+DR GE +TWQL RFYP++EELVEKL KG LP
Sbjct: 481 VHTECKKSTTG-SFPLKFDVLKTKRAARRDRVGETQTWQLSRFYPIVEELVEKLSKGHLP 540

Query: 541 KSEYSCMNEPPPSTEKPATKGTRITASQTSQSTGGPKSMRSRRTANWARTSVSDDGYGSD 600
           K +Y CMNEP P+             S +   +  P    SRRT  WAR  +SDDGY SD
Sbjct: 541 KQDYPCMNEPKPT-----------FYSGSLSPSASPVLPHSRRTPTWARRHLSDDGYFSD 600

Query: 601 SVLRHINFDSKQMGRRVFVFVIGGATRSELRVCHKLTAKLRREVVLGCSSLDDPPQYITK 660
           SVL   +   K+ G+R+FVF++GGATRSELRVCHKLT KL REV+LG SS  DP  ++TK
Sbjct: 601 SVLGRASSGFKRKGQRIFVFIVGGATRSELRVCHKLTEKLDREVILGSSSFLDPLTFLTK 653

Query: 661 LKQLSEKD 664
           +KQL+E++
Sbjct: 661 MKQLNEEE 653

BLAST of Sed0012324 vs. TAIR 10
Match: AT1G77140.1 (vacuolar protein sorting 45 )

HSP 1 Score: 110.9 bits (276), Expect = 4.0e-24
Identity = 112/512 (21.88%), Postives = 227/512 (44.34%), Query Frame = 0

Query: 41  KVLIMDRVTVKVMSHSCKMADITDQGISLVE---DLFRRRQPLPSMDAIYFVQPSKENVV 100
           KVLI+D  TV  +S     +++  + + LVE    +   ++ +  + A+YF++P+ +N+ 
Sbjct: 22  KVLILDSETVSNVSIVYSQSELLQKEVFLVEMIDSISVSKESMSHLKAVYFIRPTSDNIQ 81

Query: 101 MFLSDMSGREPLYKKAFVFFSSPVPKEFINHIKCDTSVLPRIGALREMNLEYFPIDSQAF 160
                ++   P + +  +FFS+ + K+   HI  D+     +  ++E   ++   D   F
Sbjct: 82  KLRYQLA--NPRFGEYHLFFSN-LLKDTQIHILADSDEQEVVQQVQEYYADFVSGDPYHF 141

Query: 161 ITDQEMALHDLFI--GTENSRKFDNCLNTMATRIATVFASLKEFPFVRYKVSKALDNEDD 220
             +  MA + L++     +        + +   IA VF +LK  P +RY+ +        
Sbjct: 142 TLN--MASNHLYMIPAVVDPSGLQRFSDRVVDGIAAVFLALKRRPVIRYQRTSD------ 201

Query: 221 LTEASFRELIPSKLAAAIWNCVSKYKSTIPNYPQAETCE-LLILDRSIDQIAPIIHEWTY 280
                       ++A      + +++S + ++ + E+   LL++DR  D + P++++WTY
Sbjct: 202 ---------TAKRIAHETAKLMYQHESALFDFRRTESSPLLLVIDRRDDPVTPLLNQWTY 261

Query: 281 DAMCRDLLEMEGNKYVYEVSSKTGGEPDKREAILEDTDPVWLELRHTHIADASERLHEKM 340
            AM  +L+ ++ NK   +       +      +  + D  +    + +  D    +   +
Sbjct: 262 QAMVHELIGLQDNKVDLKSIGSLPKDQQVEVVLSSEQDAFFKSNMYENFGDIGMNIKRMV 321

Query: 341 TTFTAKNKAAQIQQNARDGGEIST-RDLQKMVQALPQYTEQVEKITLHVEIAGKINKLIR 400
             F         QQ A+    I T  D+ + V   P+Y +    ++ HV +  +++KL+ 
Sbjct: 322 DDF---------QQVAKSNQNIQTVEDMARFVDNYPEYKKMQGNVSKHVTLVTEMSKLVE 381

Query: 401 EMGLRELGQLEQDLVF--GDAGAKEVINFLRTYQSGSSENKLRLLMIYASVYPEKFENDK 460
              L  + Q EQDL    G   A E +  L   +S S  ++LRL+M+YA     ++E + 
Sbjct: 382 ARKLMTVSQTEQDLACNGGQGAAYEAVTDLLNNESVSDIDRLRLVMLYAL----RYEKEN 441

Query: 461 AVKIMQL-AKLSTEDMKIVKNMQ--LLGGSDSKKATSSHSFSLKFSNQKTKQAARKDRTG 520
            V++MQL  KL++   K    +   LL  +  +K T        F N+     AR    G
Sbjct: 442 PVQLMQLFNKLASRSPKYKPGLVQFLLKQAGVEKRTGD-----LFGNRDLLNIARNMARG 495

Query: 521 EEETWQLF-RFYPMIEELVEKLCKGELPKSEY 540
            +    ++ +  P++ + +E + +G L   +Y
Sbjct: 502 LKGVENVYTQHQPLLFQTMESITRGRLRDVDY 495