Sed0010852 (gene) Chayote v1

Overview
NameSed0010852
Typegene
OrganismSechium edule (Chayote v1)
Descriptionclathrin coat assembly protein AP180
LocationLG05: 32726575 .. 32728531 (+)
RNA-Seq ExpressionSed0010852
SyntenySed0010852
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTGTCTCTTAATAACTGGCAGAAATCACATTTTCACCATTTATATTTTGATTAAGCAGTTTGTGAGGATCTGATTGAATAATTTAATTTCCGATTTGAAAATCTCCAGCCCTTCAGCATGCCAAGCAAGTTTCGAAAGGCTTTGTATGCCGTGAAAGACCAGACGAGCATAAGCCTTGCGAAGGTCTCGTCGTCTGGCGGAGGCACGAACAGAAACTCCATAGAAGTAGCCATCTTAAAAGCTACCACGCACGATAACAGTCCAATGGATCACCGGCACGTGTCGGAAATCCTCCTCTTAATCTCCGCCAGCAAATCGAATGCCGCCGCATGCGCACACTACATAGCCAAACGCATCTCCCGAACCCGCAATTGGATCGTGGCCCTAAAATCCCTAAACATAGTCCTCCGAATCTTCCAAGACGGCGACCTGCATTTCCCTCGCGAAGTCCTCCACGTGATGAAACGCGGCGCCAAGATCCTAAACCTTTCAAACTTTCGAGATGATTCCAATTCAAGCCCATGGGATTACACCGCATTCGTCCGAACCTTCGCCCTCTATCTCGACGAACGCCTCGATTGTTTCTTAACCGGAAACCTCCAAAAACGGTTCACTCAACCCAAAATCCAGAGTTACCATTTAACAACCCCGCGAATCAACCAGCCCATTCCAGACATGAAACCCGCAACCTTAATCGACCGAATTACGTACTGGCAGAAACTTTTAGACCGGGCAATCGCCACCACCCCGACCGGTCCGGCCAAGGGGAACCGGTTGGTTCTACATTCGGTTCACGCCGTGGTTCAAGAGAGCTTCGATTTGTACAAAGATATCTCCGATGGGCTTGCTTTACTTCTAGATAGCTTCTTCCATCTGCAATATCAATCCTGTGTAAATGCATTTCAAGCTTGTGTTAAAGCCGCTAAGCAATTTGAAGAATTGGGTTCGTTTTATGATTTGTGTAAAAGTATTGGGGTTGGAAGAACATCGGAATATCCAAGCGTTCAACAACCTTCCGATGAGTTAATCGAAACGTTGCAAGAATTTTTGAAAGATCAAGCTTCTTTCCCCTGCCATGGAAACCGATCCTTGCCGCTACCGCCGCCGTCCACGGCGACGAAATACAGCGACGAGCAGTCGGAGTCGTCGGAGATTAACTCGTTGGGCGATATTATGAGCGGTACCGGAAGTTGGAACTCCATCGATCACGACGGCGAAAGCGTCTCCGATTATCAACAATCCCAAATGGGCGATTTCGCAAACGAATCGAATTTTGAGTTTTTCGATGAAATCGGAAAACCCGAAGAAACTCAGTTTAGATCTGGCGATTGGGAACTCATTCTGGCGGAATCCGCGACGGAGCCGCCGCCAGAATGGCCGGATTTCTTCGGATCCATCGCCGGCGGGGATCTGTTTTCGAAACCCTTTTCTCCTCAGAATCATCACAGCAACCCGTTTCTTCAAGATTCGTTCACGATTCAGCCAGCAGAAATCACTCCGACATTTCGAGCGGAATCGATCGCCATAGCTCCGACGTTCAAAGCGGTGAATCAAAACGGGTCGGTGTTCGGAGCCTGGGATCCGAACGAATCGGGTTCGAAACAATCGGTTTCGGGTGATCCGTTTGGATTTGAAATATGTACCGAAAACGACCCGTTTGGAGACCCGTGGAGATCTGATCTGAATGGGAGTGATCGGAGTTTTGAAGTGGTGAATCAAGAGAGCTTGTTGGAGCAGCAAAAATTGTGGAAGGAACAGCAAAACAAGATTATAGCGAAGCATTTAGTGTGAGAAATAAAATAAATATTGGGATGTAAATTTGACTGTAAATATCTACGTGGAATTGATTTAAATCATAAAGTTCATTGGATAAATGAAAATTAACATTTTGGACAATTACAATTTGTACACACTTTTTTTTAGTTCTTGGGGGTCAAGAGTAGAGGTGTCCATGGC

mRNA sequence

GTTGTCTCTTAATAACTGGCAGAAATCACATTTTCACCATTTATATTTTGATTAAGCAGTTTGTGAGGATCTGATTGAATAATTTAATTTCCGATTTGAAAATCTCCAGCCCTTCAGCATGCCAAGCAAGTTTCGAAAGGCTTTGTATGCCGTGAAAGACCAGACGAGCATAAGCCTTGCGAAGGTCTCGTCGTCTGGCGGAGGCACGAACAGAAACTCCATAGAAGTAGCCATCTTAAAAGCTACCACGCACGATAACAGTCCAATGGATCACCGGCACGTGTCGGAAATCCTCCTCTTAATCTCCGCCAGCAAATCGAATGCCGCCGCATGCGCACACTACATAGCCAAACGCATCTCCCGAACCCGCAATTGGATCGTGGCCCTAAAATCCCTAAACATAGTCCTCCGAATCTTCCAAGACGGCGACCTGCATTTCCCTCGCGAAGTCCTCCACGTGATGAAACGCGGCGCCAAGATCCTAAACCTTTCAAACTTTCGAGATGATTCCAATTCAAGCCCATGGGATTACACCGCATTCGTCCGAACCTTCGCCCTCTATCTCGACGAACGCCTCGATTGTTTCTTAACCGGAAACCTCCAAAAACGGTTCACTCAACCCAAAATCCAGAGTTACCATTTAACAACCCCGCGAATCAACCAGCCCATTCCAGACATGAAACCCGCAACCTTAATCGACCGAATTACGTACTGGCAGAAACTTTTAGACCGGGCAATCGCCACCACCCCGACCGGTCCGGCCAAGGGGAACCGGTTGGTTCTACATTCGGTTCACGCCGTGGTTCAAGAGAGCTTCGATTTGTACAAAGATATCTCCGATGGGCTTGCTTTACTTCTAGATAGCTTCTTCCATCTGCAATATCAATCCTGTGTAAATGCATTTCAAGCTTGTGTTAAAGCCGCTAAGCAATTTGAAGAATTGGGTTCGTTTTATGATTTGTGTAAAAGTATTGGGGTTGGAAGAACATCGGAATATCCAAGCGTTCAACAACCTTCCGATGAGTTAATCGAAACGTTGCAAGAATTTTTGAAAGATCAAGCTTCTTTCCCCTGCCATGGAAACCGATCCTTGCCGCTACCGCCGCCGTCCACGGCGACGAAATACAGCGACGAGCAGTCGGAGTCGTCGGAGATTAACTCGTTGGGCGATATTATGAGCGGTACCGGAAGTTGGAACTCCATCGATCACGACGGCGAAAGCGTCTCCGATTATCAACAATCCCAAATGGGCGATTTCGCAAACGAATCGAATTTTGAGTTTTTCGATGAAATCGGAAAACCCGAAGAAACTCAGTTTAGATCTGGCGATTGGGAACTCATTCTGGCGGAATCCGCGACGGAGCCGCCGCCAGAATGGCCGGATTTCTTCGGATCCATCGCCGGCGGGGATCTGTTTTCGAAACCCTTTTCTCCTCAGAATCATCACAGCAACCCGTTTCTTCAAGATTCGTTCACGATTCAGCCAGCAGAAATCACTCCGACATTTCGAGCGGAATCGATCGCCATAGCTCCGACGTTCAAAGCGGTGAATCAAAACGGGTCGGTGTTCGGAGCCTGGGATCCGAACGAATCGGGTTCGAAACAATCGGTTTCGGGTGATCCGTTTGGATTTGAAATATGTACCGAAAACGACCCGTTTGGAGACCCGTGGAGATCTGATCTGAATGGGAGTGATCGGAGTTTTGAAGTGGTGAATCAAGAGAGCTTGTTGGAGCAGCAAAAATTGTGGAAGGAACAGCAAAACAAGATTATAGCGAAGCATTTAGTGTGAGAAATAAAATAAATATTGGGATGTAAATTTGACTGTAAATATCTACGTGGAATTGATTTAAATCATAAAGTTCATTGGATAAATGAAAATTAACATTTTGGACAATTACAATTTGTACACACTTTTTTTTAGTTCTTGGGGGTCAAGAGTAGAGGTGTCCATGGC

Coding sequence (CDS)

ATGCCAAGCAAGTTTCGAAAGGCTTTGTATGCCGTGAAAGACCAGACGAGCATAAGCCTTGCGAAGGTCTCGTCGTCTGGCGGAGGCACGAACAGAAACTCCATAGAAGTAGCCATCTTAAAAGCTACCACGCACGATAACAGTCCAATGGATCACCGGCACGTGTCGGAAATCCTCCTCTTAATCTCCGCCAGCAAATCGAATGCCGCCGCATGCGCACACTACATAGCCAAACGCATCTCCCGAACCCGCAATTGGATCGTGGCCCTAAAATCCCTAAACATAGTCCTCCGAATCTTCCAAGACGGCGACCTGCATTTCCCTCGCGAAGTCCTCCACGTGATGAAACGCGGCGCCAAGATCCTAAACCTTTCAAACTTTCGAGATGATTCCAATTCAAGCCCATGGGATTACACCGCATTCGTCCGAACCTTCGCCCTCTATCTCGACGAACGCCTCGATTGTTTCTTAACCGGAAACCTCCAAAAACGGTTCACTCAACCCAAAATCCAGAGTTACCATTTAACAACCCCGCGAATCAACCAGCCCATTCCAGACATGAAACCCGCAACCTTAATCGACCGAATTACGTACTGGCAGAAACTTTTAGACCGGGCAATCGCCACCACCCCGACCGGTCCGGCCAAGGGGAACCGGTTGGTTCTACATTCGGTTCACGCCGTGGTTCAAGAGAGCTTCGATTTGTACAAAGATATCTCCGATGGGCTTGCTTTACTTCTAGATAGCTTCTTCCATCTGCAATATCAATCCTGTGTAAATGCATTTCAAGCTTGTGTTAAAGCCGCTAAGCAATTTGAAGAATTGGGTTCGTTTTATGATTTGTGTAAAAGTATTGGGGTTGGAAGAACATCGGAATATCCAAGCGTTCAACAACCTTCCGATGAGTTAATCGAAACGTTGCAAGAATTTTTGAAAGATCAAGCTTCTTTCCCCTGCCATGGAAACCGATCCTTGCCGCTACCGCCGCCGTCCACGGCGACGAAATACAGCGACGAGCAGTCGGAGTCGTCGGAGATTAACTCGTTGGGCGATATTATGAGCGGTACCGGAAGTTGGAACTCCATCGATCACGACGGCGAAAGCGTCTCCGATTATCAACAATCCCAAATGGGCGATTTCGCAAACGAATCGAATTTTGAGTTTTTCGATGAAATCGGAAAACCCGAAGAAACTCAGTTTAGATCTGGCGATTGGGAACTCATTCTGGCGGAATCCGCGACGGAGCCGCCGCCAGAATGGCCGGATTTCTTCGGATCCATCGCCGGCGGGGATCTGTTTTCGAAACCCTTTTCTCCTCAGAATCATCACAGCAACCCGTTTCTTCAAGATTCGTTCACGATTCAGCCAGCAGAAATCACTCCGACATTTCGAGCGGAATCGATCGCCATAGCTCCGACGTTCAAAGCGGTGAATCAAAACGGGTCGGTGTTCGGAGCCTGGGATCCGAACGAATCGGGTTCGAAACAATCGGTTTCGGGTGATCCGTTTGGATTTGAAATATGTACCGAAAACGACCCGTTTGGAGACCCGTGGAGATCTGATCTGAATGGGAGTGATCGGAGTTTTGAAGTGGTGAATCAAGAGAGCTTGTTGGAGCAGCAAAAATTGTGGAAGGAACAGCAAAACAAGATTATAGCGAAGCATTTAGTGTGA

Protein sequence

MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILLLISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAKILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQKRFTQPKIQSYHLTTPRINQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFDLYKDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPSDELIETLQEFLKDQASFPCHGNRSLPLPPPSTATKYSDEQSESSEINSLGDIMSGTGSWNSIDHDGESVSDYQQSQMGDFANESNFEFFDEIGKPEETQFRSGDWELILAESATEPPPEWPDFFGSIAGGDLFSKPFSPQNHHSNPFLQDSFTIQPAEITPTFRAESIAIAPTFKAVNQNGSVFGAWDPNESGSKQSVSGDPFGFEICTENDPFGDPWRSDLNGSDRSFEVVNQESLLEQQKLWKEQQNKIIAKHLV
Homology
BLAST of Sed0010852 vs. NCBI nr
Match: XP_038887470.1 (clathrin coat assembly protein AP180 [Benincasa hispida])

HSP 1 Score: 801.6 bits (2069), Expect = 4.3e-228
Identity = 435/607 (71.66%), Postives = 484/607 (79.74%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILL 60
           MPSK RKALYAVKDQTSI LAKVSSS GG N  S+EVA+LKATTHD+ P+DHR+VSEIL 
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSAGGPNATSLEVAVLKATTHDDGPLDHRYVSEILQ 60

Query: 61  LISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAK 120
           LISA+KSNAAACAH IAKRI++TRNWIVALKSLN+VL+IFQDGD +FPREVLH MKRGAK
Sbjct: 61  LISANKSNAAACAHSIAKRITKTRNWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQKRFTQPKIQSYHLTTPRI 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTG LQKRFTQ KI+SYH TT RI
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKIESYHGTTRRI 180

Query: 181 NQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFDLYKDIS 240
           N+PI DMKPA LIDRITYWQKLLDRAIAT PTGPAKGNRLV HS+HAVVQESFDLY+DI+
Sbjct: 181 NEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDIT 240

Query: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300
           DGLALLLDSFFHLQYQSCVNAFQACVKA KQFEELGSFYDLCKSIGVGRTSEYPSVQQPS
Sbjct: 241 DGLALLLDSFFHLQYQSCVNAFQACVKATKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300

Query: 301 DELIETLQEFLKDQASFPCHGNRSLP----LPPPSTATKYSDEQSES--------SEINS 360
           DELIETLQEFLKDQASFPCHG+RS P    LP P+T      EQSES        S++NS
Sbjct: 301 DELIETLQEFLKDQASFPCHGSRSPPPQAILPGPTTKLTDDLEQSESSDKNSERGSDLNS 360

Query: 361 LGDIMSGTGSW----NSIDHDGESVSDYQ---QSQMGDFANES----NFEFFDEIGKP-E 420
           +GDIMS TGSW    NS++ DGE  SDYQ   QS++GDFANES    NF FF+E G P +
Sbjct: 361 VGDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANESNLSPNFAFFEENGTPFQ 420

Query: 421 ETQF------RSGDWELILAESATEPPPEWPDFFGSIAGGDLFSKPFSPQNHHSNPFLQD 480
           E QF      R GDWEL+LAESAT  P EWPDFF    G DLF+KPFSPQ+H++NPFLQD
Sbjct: 421 EPQFGATPSVRFGDWELVLAESATGSPQEWPDFFSPSIGDDLFAKPFSPQHHYNNPFLQD 480

Query: 481 S---FTIQPA-----EITPTFRA----ESIAIAPTFKA-------VNQNGSVFGAWDPNE 540
           S     + P      EI PTFRA    E++++APTF+A        NQN S+F AW    
Sbjct: 481 SDDLLAVPPTFKAEREIAPTFRAQNSKETVSVAPTFRAETATLSSKNQNSSMFEAW---- 540

Query: 541 SGSKQSVSGDPFGF-EICTENDPFGDPWRSDLNGSDRSFEVVNQESLLEQQKLWKEQQNK 558
            G+ Q  +G  FGF ++  ENDPF  PW  +LNG+D +F+   QESL++QQKLWKEQQNK
Sbjct: 541 -GTVQ--NGRGFGFDDLGVENDPFAGPWSRNLNGNDPNFD---QESLMQQQKLWKEQQNK 597

BLAST of Sed0010852 vs. NCBI nr
Match: XP_008449479.1 (PREDICTED: clathrin coat assembly protein AP180 [Cucumis melo] >KAA0061650.1 clathrin coat assembly protein AP180 [Cucumis melo var. makuwa] >TYK05856.1 clathrin coat assembly protein AP180 [Cucumis melo var. makuwa])

HSP 1 Score: 773.9 bits (1997), Expect = 9.7e-220
Identity = 421/585 (71.97%), Postives = 461/585 (78.80%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILL 60
           MPSK RKALYAVKDQTSI LAKVSSS GG N  S+EVA+LKATTHD+ P+DHR+V+EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSAGGPNATSLEVAVLKATTHDDVPLDHRYVNEILT 60

Query: 61  LISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAK 120
           LISA+KS+AAACAH IAKRI++TRNW VALKSLN+VL+IFQDGD +FPREVLH MKRGAK
Sbjct: 61  LISANKSHAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQKRFTQPKIQSYHLTTPRI 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTG LQKRFTQ K +SYH TT RI
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTESYHATTRRI 180

Query: 181 NQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFDLYKDIS 240
           N+PI DMKPA LIDRITYWQKLLDRAIAT PTGPAKGNRLV HS+HAVVQESFDLY+DIS
Sbjct: 181 NEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDIS 240

Query: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300
           DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS
Sbjct: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300

Query: 301 DELIETLQEFLKDQASFPCHGNRSLP---LPPPSTATKYSDEQSES--------SEINSL 360
           DELIETLQEFLKDQASFPCHGNRS P   L  P T      EQSES        S+ NS+
Sbjct: 301 DELIETLQEFLKDQASFPCHGNRSPPQAILSVPMTKLTDDLEQSESSDKNSERGSDFNSV 360

Query: 361 GDIMSGTGSW----NSIDHDGESVSDYQ---QSQMGDFANES----NFEFFDEIGKP--E 420
           GDIMS TGSW    NS++ DGE  SDYQ   QS++GDFANES    NF FF+E G P  E
Sbjct: 361 GDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANESNLSPNFAFFEENGMPFEE 420

Query: 421 ETQF------RSGDWELILAESATEPPPEWPDFFGSIAGGDLFSKPFSPQNHHSNPFLQD 480
           E+QF      R GDWEL+LAESAT  P EWPDFF    G DLF+KPFSPQ+H+ NPFLQD
Sbjct: 421 ESQFGVAPSLRFGDWELVLAESATASPKEWPDFFSPSIGDDLFAKPFSPQHHYKNPFLQD 480

Query: 481 SFTIQPAEITPTFRAESIAIAPTFKAVNQNGSVFGAWDPNESGSKQSVSGDPFGFEICTE 540
           S  +  A   PTF+AE   + PTF+A N     F A     S   ++   D  GF     
Sbjct: 481 SDDLFTA--PPTFKAEK-EMGPTFRAENSVAPTFRAETATLSSQNENRGFDELGF----G 540

Query: 541 NDPFGDPWRSDLNGSDRSFEVVNQESLLEQQKLWKEQQNKIIAKH 556
           NDPF  P    LNG+D+SF+   +ESLL+QQKLWKEQQNKIIAKH
Sbjct: 541 NDPFAGP----LNGNDQSFD---EESLLQQQKLWKEQQNKIIAKH 571

BLAST of Sed0010852 vs. NCBI nr
Match: XP_004140244.1 (clathrin coat assembly protein AP180 [Cucumis sativus] >KGN47964.1 hypothetical protein Csa_003702 [Cucumis sativus])

HSP 1 Score: 772.7 bits (1994), Expect = 2.2e-219
Identity = 418/585 (71.45%), Postives = 463/585 (79.15%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILL 60
           MPSK RKALYAVKDQTSI LAKVSSS GG N  S+EV +LKATTHD+SP+DHR+V+EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSAGGPNATSLEVVVLKATTHDDSPLDHRYVTEILT 60

Query: 61  LISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAK 120
           LISA+KSNAAACAH IAKRI++TRNW VALKSLN+VL+IFQDGD +FPREVLH MKRGAK
Sbjct: 61  LISANKSNAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQKRFTQPKIQSYHLTTPRI 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTG LQKRFTQ K +SYH TT RI
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKAESYHATTRRI 180

Query: 181 NQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFDLYKDIS 240
           N+PI DMKPA LIDRITYWQKLLDRAIAT PTGPAKGNRLV HS+HAVVQESFDLY+DIS
Sbjct: 181 NEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDIS 240

Query: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300
           DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS
Sbjct: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300

Query: 301 DELIETLQEFLKDQASFPCHGNRSLPLP-PPSTATKYSD--EQSES--------SEINSL 360
           DELIETLQEFLKDQASFPCHGNRS P P  P + TK++D  +QSES        S+ NS+
Sbjct: 301 DELIETLQEFLKDQASFPCHGNRSPPQPILPGSITKFTDDLDQSESSDKNSERGSDFNSV 360

Query: 361 GDIMSGTGSW----NSIDHDGESVSDYQ---QSQMGDFANE----SNFEFFDEIGK--PE 420
           GDIMS TGSW    NS++ DGE  SDYQ   QS++GDFANE     NF FF+E G    E
Sbjct: 361 GDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANELNLSPNFAFFEESGMAFEE 420

Query: 421 ETQF------RSGDWELILAESATEPPPEWPDFFGSIAGGDLFSKPFSPQNHHSNPFLQD 480
           E+QF      R GDWEL+LAESAT+ P EWPDFF    G +L++KPFSPQ+H+ NPFLQD
Sbjct: 421 ESQFGVAPSMRFGDWELVLAESATDSPKEWPDFFSPSIGDELYAKPFSPQHHYKNPFLQD 480

Query: 481 SFTIQPAEITPTFRAESIAIAPTFKAVNQNGSVFGAWDPNESGSKQSVSGDPFGFEICTE 540
           S  +  A   PTF+AE   + PTF+A N     F A     S   ++   D  GF     
Sbjct: 481 SDDLFTA--PPTFKAEK-EMGPTFRAENSVAPTFRAETATLSSQNENCGFDELGF----G 540

Query: 541 NDPFGDPWRSDLNGSDRSFEVVNQESLLEQQKLWKEQQNKIIAKH 556
           NDPF  P    LNG+D  F+   +ESLL+QQKLWKEQQNKIIAKH
Sbjct: 541 NDPFAGP----LNGNDPIFD---EESLLQQQKLWKEQQNKIIAKH 571

BLAST of Sed0010852 vs. NCBI nr
Match: XP_023553928.1 (clathrin coat assembly protein AP180-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 767.7 bits (1981), Expect = 6.9e-218
Identity = 419/608 (68.91%), Postives = 475/608 (78.12%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILL 60
           MPSK RKALYAVKDQTSISLAKVSSS GG N  S+EVAILKATTHD+ P++ R+V+EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSISLAKVSSSTGGPNAISLEVAILKATTHDDVPLEQRYVAEILQ 60

Query: 61  LISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAK 120
           LISA+KS+AA CAH IAKRI++TR+WIVALKSLN+VL+IFQDGD +FPREVLH MKRGAK
Sbjct: 61  LISANKSHAAVCAHSIAKRINKTRHWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQKRFTQPKIQSYHLTTPRI 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTG LQKRFT+ K +S+H  T RI
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTERKTESFHGNTRRI 180

Query: 181 NQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFDLYKDIS 240
           N+PI DMKPA LIDRI YWQKLLDRAIAT PTGPAKGNRLVL+S+HAVVQESFDLY+DIS
Sbjct: 181 NEPIRDMKPAMLIDRIYYWQKLLDRAIATRPTGPAKGNRLVLNSLHAVVQESFDLYRDIS 240

Query: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300
           DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS
Sbjct: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300

Query: 301 DELIETLQEFLKDQASFPCHGNRSLPLPPPSTAT----KYSD--EQSESS--------EI 360
           DELIETLQEFLKDQASFPCH +RS P P   + T    K +D  EQSESS        + 
Sbjct: 301 DELIETLQEFLKDQASFPCHDSRSPPAPVILSGTISTMKLTDDLEQSESSDKNSERELDF 360

Query: 361 NSLGDIMSGTGSW----NSIDHDGESVSDYQ---QSQMGDFANES----NFEFFDEIGKP 420
           NS G++MS TGSW    NS++ DGE  SDYQ   QS++GDFANES    NF FFD+   P
Sbjct: 361 NSFGEMMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANESNLSPNFAFFDDDETP 420

Query: 421 --EETQF------RSGDWELILAESATEPPPEWPDFFGSIAGGDLFSKPF-SPQNHHSNP 480
             EE++F      R GDWEL+LAESAT+   EWPDFF    G D F KPF SPQ+ ++NP
Sbjct: 421 FQEESEFGATPGLRCGDWELVLAESATDTAEEWPDFFAPSIGDDPFEKPFTSPQHQYNNP 480

Query: 481 FLQD---SFTIQP-----AEITPTFRA----ESIAIAPTFK-------AVNQNGSVFGAW 540
           FLQD   SF + P      EI PTFRA    E++ +APTF+       + NQNGS+F  W
Sbjct: 481 FLQDSNESFAVPPTFKAETEIAPTFRAENVKEAVVVAPTFRVETATFSSQNQNGSMFDDW 540

Query: 541 DPNESGSKQSVSGDPFGFEICTENDPFGDPWRSDLNGSDRSFEVVNQESLLEQQKLWKEQ 556
            P  +G     SG  F F++  ENDPF + W S+LNG++ S  V++QESLL+QQKLWKE 
Sbjct: 541 GPIPNG-----SGSGFEFDLGAENDPFAEAWDSNLNGNEPSLNVMDQESLLQQQKLWKEH 600

BLAST of Sed0010852 vs. NCBI nr
Match: KAG6572416.1 (Clathrin coat assembly protein 180, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012015.1 Clathrin coat assembly protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 767.3 bits (1980), Expect = 9.0e-218
Identity = 419/608 (68.91%), Postives = 475/608 (78.12%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILL 60
           MPSK RKALYAVKDQTSISLAKVSSS GG N  S+EVAILKATTHD+ P++ R+V+EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSISLAKVSSSTGGPNAISLEVAILKATTHDDVPLEQRYVAEILQ 60

Query: 61  LISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAK 120
           LISA+KS+AA CAH IAKRI++TR+WIVALKSLN+VL+IFQDGD +FPREVLH MKRGAK
Sbjct: 61  LISANKSHAAVCAHSIAKRINKTRHWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQKRFTQPKIQSYHLTTPRI 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTG LQKRFT+ K +S+H  T RI
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTERKTESFHGNTRRI 180

Query: 181 NQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFDLYKDIS 240
           N+PI DMKPA LIDRI YWQKLLDRAIAT PTGPAKGNRLVL+S+HAVVQESFDLY+DIS
Sbjct: 181 NEPIRDMKPAMLIDRIYYWQKLLDRAIATRPTGPAKGNRLVLNSLHAVVQESFDLYRDIS 240

Query: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300
           DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS
Sbjct: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300

Query: 301 DELIETLQEFLKDQASFPCHGNRSLPLPPPSTAT----KYSD--EQSESS--------EI 360
           DELIETLQEFLKDQASFPCH +RS P P   + T    K +D  EQSESS        + 
Sbjct: 301 DELIETLQEFLKDQASFPCHDSRSPPAPVILSGTISTMKLTDDLEQSESSDKNSERELDF 360

Query: 361 NSLGDIMSGTGSW----NSIDHDGESVSDYQ---QSQMGDFANES----NFEFFDEIGKP 420
           NS G++MS TGSW    NS++ DGE  SDYQ   QS++GDFANES    NF FF++   P
Sbjct: 361 NSFGEMMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANESNLSPNFAFFEDDETP 420

Query: 421 --EETQ------FRSGDWELILAESATEPPPEWPDFFGSIAGGDLFSKPF-SPQNHHSNP 480
             EET+       R GDWEL+LAESAT+   EWPDFF    G D F KPF SPQ+ ++NP
Sbjct: 421 FQEETELGAARGLRCGDWELVLAESATDTAEEWPDFFAPSIGDDPFEKPFTSPQHQYNNP 480

Query: 481 FLQD---SFTIQP-----AEITPTFRA----ESIAIAPTFK-------AVNQNGSVFGAW 540
           FLQD   SF + P      EI PTFRA    E++ +APTF+       + NQNGS+F  W
Sbjct: 481 FLQDSNESFAVPPTFKAETEIAPTFRAENVKEAVVVAPTFRVETPAFSSQNQNGSMFDDW 540

Query: 541 DPNESGSKQSVSGDPFGFEICTENDPFGDPWRSDLNGSDRSFEVVNQESLLEQQKLWKEQ 556
            P  +G     SG  F F++  ENDPF + W S+LNG++ S  V++QESLL+QQKLWKEQ
Sbjct: 541 GPIPNG-----SGSGFEFDLGAENDPFAEAWDSNLNGNEPSLNVMDQESLLQQQKLWKEQ 600

BLAST of Sed0010852 vs. ExPASy Swiss-Prot
Match: Q9ZVN6 (Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE=1 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.1e-128
Identity = 309/670 (46.12%), Postives = 386/670 (57.61%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSG-GGTNRNSIEVAILKATTHDNS-PMDHRHVSEI 60
           MPSK +KA+ AVKDQTSISLAKV++   GG +  ++EVAILKAT+HD   P+D R V+EI
Sbjct: 1   MPSKLKKAIGAVKDQTSISLAKVANGATGGGDLTTLEVAILKATSHDEEVPIDDRLVTEI 60

Query: 61  LLLISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRG 120
           L +IS+ KS+AA+CA  I +RI RTRNWIVALKSL +VLRIFQDGD +FPREVLH MKRG
Sbjct: 61  LGIISSKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKRG 120

Query: 121 AKILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQKRFTQ----PKIQSYH 180
           AKILNLS+FRDDSNS PWD+TAFVRTFALYLDERLDCFLTG LQ+R+T      +I +  
Sbjct: 121 AKILNLSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKLQRRYTNREQTGRISTNS 180

Query: 181 LTTPRIN---------QPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSV 240
            T  R N           + DMKP  L+D+ITYWQKLLDRAIAT PTG AK NRLV  S+
Sbjct: 181 TTRSRFNPKAGIKSHEPAVRDMKPVMLLDKITYWQKLLDRAIATRPTGDAKANRLVKMSL 240

Query: 241 HAVVQESFDLYKDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSI 300
           +AV+QESFDLY+DISDGLALLLDSFFHLQYQSC+NAFQACV+A+KQFEEL +FYDL KSI
Sbjct: 241 YAVMQESFDLYRDISDGLALLLDSFFHLQYQSCINAFQACVRASKQFEELNAFYDLSKSI 300

Query: 301 GVGRTSEYPSVQQPSDELIETLQEFLKDQASFPCHG------NRSLPLPPPS-------- 360
           G+GRTSEYPS+Q+ S EL+ETLQEFLKDQ+SFP         N  LP PP S        
Sbjct: 301 GIGRTSEYPSIQKISLELLETLQEFLKDQSSFPASSGLYPSPNSFLPPPPSSKDSAVSSS 360

Query: 361 ---------TATKYSDEQSESSEINSLGDIMS----GTGS------------------WN 420
                    T+ +YSD  S  S   SL D+MS    GT S                   N
Sbjct: 361 LDFGDSTIDTSERYSDYGSFRS--TSLEDLMSRTEAGTSSPPMSCHSEPYGGGRDDPNGN 420

Query: 421 SID-------------------------HDGESVSDYQQSQMGDFANESNFEFFD----- 480
           + D                          D  + ++ +  +     ++S  E FD     
Sbjct: 421 NFDTVSTKSLPNNPSVSASNLILDLLSLDDVSNTAEAEDVEDKKKQDDSKAETFDPWEAL 480

Query: 481 ---------------EIGKPEETQFRSGDWELILAESATEPPPEWPDFFGSIA----GGD 540
                          E    E+ Q  SG+W L L E+AT+          S+A    G D
Sbjct: 481 MLRDDPKKKIETIEEEPSTAEDHQRDSGNWLLALEETATQVQGN-----NSMAIVPFGLD 540

Query: 541 LFSKPFSPQNHHSNPFLQDSFTIQPAEITPTFRAESIAIA-----PTFKAVNQNGSVFGA 557
                F       NPFL++          P      +A+      PTF+      +V   
Sbjct: 541 DPMPAFQAATDQYNPFLEEPVAQLATAGEPMITFGGLALTGFQPEPTFQV-----NVPDD 600

BLAST of Sed0010852 vs. ExPASy Swiss-Prot
Match: Q8S9J8 (Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=At4g32285 PE=1 SV=2)

HSP 1 Score: 258.8 bits (660), Expect = 1.4e-67
Identity = 154/395 (38.99%), Postives = 232/395 (58.73%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILL 60
           M    RKA+  VKDQTSI +AKV+S+        +EVAI+KAT+HD+     +++ EIL 
Sbjct: 1   MALSMRKAIGVVKDQTSIGIAKVASN----MAPDLEVAIVKATSHDDDQSSDKYIREILS 60

Query: 61  LISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAK 120
           L S S+    AC   +++R+ +TR+WIVALK+L +V R+  +GD  F  E+L+  +RG +
Sbjct: 61  LTSLSRGYVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTR 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFL--------------------TGN 180
           ILN+S+FRD+++SS WD++AFVRT+A YLD+RL+  L                     G 
Sbjct: 121 ILNMSDFRDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGY 180

Query: 181 LQKR--FTQPKIQSYHLTT------PR-------INQ-----------PIPDMKPATLID 240
            + R  F  P  ++Y   T      P+       +N+           P+ +M P  +  
Sbjct: 181 NRSRDDFRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFG 240

Query: 241 RITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFDLYKDISDGLALLLDSFFHLQ 300
           ++ + Q+LLDR ++  PTG AK +R++L +++ VV+ESF LY DI + LA+LLD FF ++
Sbjct: 241 KMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDME 300

Query: 301 YQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPSDELIETLQEFLKDQ 350
           Y  CV AF A   AAKQ +EL +FY  CK  GV R+SEYP VQ+ + +L+ETL+EF++D+
Sbjct: 301 YTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDR 360

BLAST of Sed0010852 vs. ExPASy Swiss-Prot
Match: Q8LF20 (Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=At2g25430 PE=1 SV=2)

HSP 1 Score: 256.9 bits (655), Expect = 5.2e-67
Identity = 151/416 (36.30%), Postives = 232/416 (55.77%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILL 60
           M    RKA+ AVKDQTSI +AKV+S+        +EVAI+KAT+HD+ P   +++ EIL 
Sbjct: 1   MAPSIRKAIGAVKDQTSIGIAKVASN----MAPDLEVAIVKATSHDDDPASEKYIREILN 60

Query: 61  LISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAK 120
           L S S+    AC   +++R+S+TR+W+VALK+L +V R+  +GD  F  E+L+  +RG +
Sbjct: 61  LTSLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTR 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFL----------------------- 180
           +LN+S+FRD+++SS WD++AFVRT+A YLD+RL+  L                       
Sbjct: 121 MLNMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDD 180

Query: 181 -TGNLQKRFTQPKIQSYHLTT--------------------------------------- 240
             G  +  F  P  +SY                                           
Sbjct: 181 RYGRGRDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGGG 240

Query: 241 ---PRINQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFD 300
               ++  P+ +M P  +  ++ + Q+LLDR ++  PTG AK +R++L +++ VV+ESF 
Sbjct: 241 RDEKKVVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFK 300

Query: 301 LYKDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYP 350
           LY DI + LA+LLD FF ++Y  CV AF A   AAKQ +EL +FY+ CK  GV R+SEYP
Sbjct: 301 LYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYP 360

BLAST of Sed0010852 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 252.3 bits (643), Expect = 1.3e-65
Identity = 132/371 (35.58%), Postives = 225/371 (60.65%), Query Frame = 0

Query: 3   SKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILLLI 62
           SK ++A+ AVKDQTS+ LAKV   G  ++   +E+A++KAT HD+ P + +++ EIL L 
Sbjct: 4   SKLKRAIGAVKDQTSVGLAKV--GGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCLT 63

Query: 63  SASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAKIL 122
           S S++  +AC   +++R+++T+NW VALK+L ++ R+  DGD  + +E+    +RG ++L
Sbjct: 64  SYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRLL 123

Query: 123 NLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQK------------------- 182
           N+S+FRD S S  WDY+AFVRT+ALYLDERLD  + G   K                   
Sbjct: 124 NMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEEDDH 183

Query: 183 RFTQPKIQSYHLTTPRINQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLH 242
           R T   I+S  +     ++P+ +MK   + +R+ + Q+LLDR +A  PTG AK NR+V+ 
Sbjct: 184 RGTSNDIRSKAIVVK--SKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIV 243

Query: 243 SVHAVVQESFDLYKDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCK 302
           +++ +V+ESF LY +I++ + +L++ F  L     +  ++   + +KQF+EL  FY  CK
Sbjct: 244 AMYPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCK 303

Query: 303 SIGVGRTSEYPSVQQPSDELIETLQEFLKDQASFPCH-----GNRSLPLPPPSTATKYSD 350
           ++ V R+SEYP +++ + + ++ + EF++D+++           RS       + T+Y  
Sbjct: 304 NMAVARSSEYPELEKITQKKLDLMDEFIRDKSALAAQTTKSSSKRSNKSEEEESKTEYIQ 363

BLAST of Sed0010852 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 6.4e-65
Identity = 129/357 (36.13%), Postives = 224/357 (62.75%), Query Frame = 0

Query: 3   SKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILLLI 62
           SKF++A+ AVKDQTS+ LAKV  +G   + + ++VAI+KAT H+  P + +++ EIL L 
Sbjct: 4   SKFKRAIGAVKDQTSVGLAKV--NGRSASLSELDVAIVKATRHEEFPAEEKYIREILSLT 63

Query: 63  SASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAKIL 122
           S S+S   AC   +++R+++T+ W VALK+L ++ R+  +GD  + +E+    +RG ++L
Sbjct: 64  SYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRLL 123

Query: 123 NLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQKRFT----------QPKIQS 182
           N+S+FRD S S+ WDY+AFVRT+ALYLDERLD  +     KR            +    +
Sbjct: 124 NMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGEADEEEQDQAA 183

Query: 183 YHLTTPRI--NQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQ 242
             L+T  +  +QPI +MK   +  RI + Q+LLDR +A  PTG A+ NR+V+ +++ +V+
Sbjct: 184 ADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPIVK 243

Query: 243 ESFDLYKDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRT 302
           ESF +Y D+++ + +L++ F  L     +  +    + +KQFEEL  FY  CK++G+ R+
Sbjct: 244 ESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIARS 303

Query: 303 SEYPSVQQPSDELIETLQEFLKDQASFPCHGNRSLPLPPPSTATKYSDEQSESSEIN 348
           SEYP +++ + + ++ + EF++D+++   H  +S  +    +     D+++ + E+N
Sbjct: 304 SEYPEIEKITQKKLDLMDEFIRDKSALE-HTKQSKSV---KSEADEDDDEARTEEVN 354

BLAST of Sed0010852 vs. ExPASy TrEMBL
Match: A0A5D3C685 (Clathrin coat assembly protein AP180 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1465G00110 PE=4 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 4.7e-220
Identity = 421/585 (71.97%), Postives = 461/585 (78.80%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILL 60
           MPSK RKALYAVKDQTSI LAKVSSS GG N  S+EVA+LKATTHD+ P+DHR+V+EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSAGGPNATSLEVAVLKATTHDDVPLDHRYVNEILT 60

Query: 61  LISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAK 120
           LISA+KS+AAACAH IAKRI++TRNW VALKSLN+VL+IFQDGD +FPREVLH MKRGAK
Sbjct: 61  LISANKSHAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQKRFTQPKIQSYHLTTPRI 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTG LQKRFTQ K +SYH TT RI
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTESYHATTRRI 180

Query: 181 NQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFDLYKDIS 240
           N+PI DMKPA LIDRITYWQKLLDRAIAT PTGPAKGNRLV HS+HAVVQESFDLY+DIS
Sbjct: 181 NEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDIS 240

Query: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300
           DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS
Sbjct: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300

Query: 301 DELIETLQEFLKDQASFPCHGNRSLP---LPPPSTATKYSDEQSES--------SEINSL 360
           DELIETLQEFLKDQASFPCHGNRS P   L  P T      EQSES        S+ NS+
Sbjct: 301 DELIETLQEFLKDQASFPCHGNRSPPQAILSVPMTKLTDDLEQSESSDKNSERGSDFNSV 360

Query: 361 GDIMSGTGSW----NSIDHDGESVSDYQ---QSQMGDFANES----NFEFFDEIGKP--E 420
           GDIMS TGSW    NS++ DGE  SDYQ   QS++GDFANES    NF FF+E G P  E
Sbjct: 361 GDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANESNLSPNFAFFEENGMPFEE 420

Query: 421 ETQF------RSGDWELILAESATEPPPEWPDFFGSIAGGDLFSKPFSPQNHHSNPFLQD 480
           E+QF      R GDWEL+LAESAT  P EWPDFF    G DLF+KPFSPQ+H+ NPFLQD
Sbjct: 421 ESQFGVAPSLRFGDWELVLAESATASPKEWPDFFSPSIGDDLFAKPFSPQHHYKNPFLQD 480

Query: 481 SFTIQPAEITPTFRAESIAIAPTFKAVNQNGSVFGAWDPNESGSKQSVSGDPFGFEICTE 540
           S  +  A   PTF+AE   + PTF+A N     F A     S   ++   D  GF     
Sbjct: 481 SDDLFTA--PPTFKAEK-EMGPTFRAENSVAPTFRAETATLSSQNENRGFDELGF----G 540

Query: 541 NDPFGDPWRSDLNGSDRSFEVVNQESLLEQQKLWKEQQNKIIAKH 556
           NDPF  P    LNG+D+SF+   +ESLL+QQKLWKEQQNKIIAKH
Sbjct: 541 NDPFAGP----LNGNDQSFD---EESLLQQQKLWKEQQNKIIAKH 571

BLAST of Sed0010852 vs. ExPASy TrEMBL
Match: A0A1S3BN17 (clathrin coat assembly protein AP180 OS=Cucumis melo OX=3656 GN=LOC103491352 PE=4 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 4.7e-220
Identity = 421/585 (71.97%), Postives = 461/585 (78.80%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILL 60
           MPSK RKALYAVKDQTSI LAKVSSS GG N  S+EVA+LKATTHD+ P+DHR+V+EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSAGGPNATSLEVAVLKATTHDDVPLDHRYVNEILT 60

Query: 61  LISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAK 120
           LISA+KS+AAACAH IAKRI++TRNW VALKSLN+VL+IFQDGD +FPREVLH MKRGAK
Sbjct: 61  LISANKSHAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQKRFTQPKIQSYHLTTPRI 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTG LQKRFTQ K +SYH TT RI
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTESYHATTRRI 180

Query: 181 NQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFDLYKDIS 240
           N+PI DMKPA LIDRITYWQKLLDRAIAT PTGPAKGNRLV HS+HAVVQESFDLY+DIS
Sbjct: 181 NEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDIS 240

Query: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300
           DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS
Sbjct: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300

Query: 301 DELIETLQEFLKDQASFPCHGNRSLP---LPPPSTATKYSDEQSES--------SEINSL 360
           DELIETLQEFLKDQASFPCHGNRS P   L  P T      EQSES        S+ NS+
Sbjct: 301 DELIETLQEFLKDQASFPCHGNRSPPQAILSVPMTKLTDDLEQSESSDKNSERGSDFNSV 360

Query: 361 GDIMSGTGSW----NSIDHDGESVSDYQ---QSQMGDFANES----NFEFFDEIGKP--E 420
           GDIMS TGSW    NS++ DGE  SDYQ   QS++GDFANES    NF FF+E G P  E
Sbjct: 361 GDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANESNLSPNFAFFEENGMPFEE 420

Query: 421 ETQF------RSGDWELILAESATEPPPEWPDFFGSIAGGDLFSKPFSPQNHHSNPFLQD 480
           E+QF      R GDWEL+LAESAT  P EWPDFF    G DLF+KPFSPQ+H+ NPFLQD
Sbjct: 421 ESQFGVAPSLRFGDWELVLAESATASPKEWPDFFSPSIGDDLFAKPFSPQHHYKNPFLQD 480

Query: 481 SFTIQPAEITPTFRAESIAIAPTFKAVNQNGSVFGAWDPNESGSKQSVSGDPFGFEICTE 540
           S  +  A   PTF+AE   + PTF+A N     F A     S   ++   D  GF     
Sbjct: 481 SDDLFTA--PPTFKAEK-EMGPTFRAENSVAPTFRAETATLSSQNENRGFDELGF----G 540

Query: 541 NDPFGDPWRSDLNGSDRSFEVVNQESLLEQQKLWKEQQNKIIAKH 556
           NDPF  P    LNG+D+SF+   +ESLL+QQKLWKEQQNKIIAKH
Sbjct: 541 NDPFAGP----LNGNDQSFD---EESLLQQQKLWKEQQNKIIAKH 571

BLAST of Sed0010852 vs. ExPASy TrEMBL
Match: A0A0A0KGJ5 (ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G421580 PE=4 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 1.0e-219
Identity = 418/585 (71.45%), Postives = 463/585 (79.15%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILL 60
           MPSK RKALYAVKDQTSI LAKVSSS GG N  S+EV +LKATTHD+SP+DHR+V+EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSAGGPNATSLEVVVLKATTHDDSPLDHRYVTEILT 60

Query: 61  LISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAK 120
           LISA+KSNAAACAH IAKRI++TRNW VALKSLN+VL+IFQDGD +FPREVLH MKRGAK
Sbjct: 61  LISANKSNAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQKRFTQPKIQSYHLTTPRI 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTG LQKRFTQ K +SYH TT RI
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKAESYHATTRRI 180

Query: 181 NQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFDLYKDIS 240
           N+PI DMKPA LIDRITYWQKLLDRAIAT PTGPAKGNRLV HS+HAVVQESFDLY+DIS
Sbjct: 181 NEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDIS 240

Query: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300
           DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS
Sbjct: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300

Query: 301 DELIETLQEFLKDQASFPCHGNRSLPLP-PPSTATKYSD--EQSES--------SEINSL 360
           DELIETLQEFLKDQASFPCHGNRS P P  P + TK++D  +QSES        S+ NS+
Sbjct: 301 DELIETLQEFLKDQASFPCHGNRSPPQPILPGSITKFTDDLDQSESSDKNSERGSDFNSV 360

Query: 361 GDIMSGTGSW----NSIDHDGESVSDYQ---QSQMGDFANE----SNFEFFDEIGK--PE 420
           GDIMS TGSW    NS++ DGE  SDYQ   QS++GDFANE     NF FF+E G    E
Sbjct: 361 GDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANELNLSPNFAFFEESGMAFEE 420

Query: 421 ETQF------RSGDWELILAESATEPPPEWPDFFGSIAGGDLFSKPFSPQNHHSNPFLQD 480
           E+QF      R GDWEL+LAESAT+ P EWPDFF    G +L++KPFSPQ+H+ NPFLQD
Sbjct: 421 ESQFGVAPSMRFGDWELVLAESATDSPKEWPDFFSPSIGDELYAKPFSPQHHYKNPFLQD 480

Query: 481 SFTIQPAEITPTFRAESIAIAPTFKAVNQNGSVFGAWDPNESGSKQSVSGDPFGFEICTE 540
           S  +  A   PTF+AE   + PTF+A N     F A     S   ++   D  GF     
Sbjct: 481 SDDLFTA--PPTFKAEK-EMGPTFRAENSVAPTFRAETATLSSQNENCGFDELGF----G 540

Query: 541 NDPFGDPWRSDLNGSDRSFEVVNQESLLEQQKLWKEQQNKIIAKH 556
           NDPF  P    LNG+D  F+   +ESLL+QQKLWKEQQNKIIAKH
Sbjct: 541 NDPFAGP----LNGNDPIFD---EESLLQQQKLWKEQQNKIIAKH 571

BLAST of Sed0010852 vs. ExPASy TrEMBL
Match: A0A6J1I2N8 (clathrin coat assembly protein AP180-like OS=Cucurbita maxima OX=3661 GN=LOC111468453 PE=4 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 4.4e-218
Identity = 419/608 (68.91%), Postives = 475/608 (78.12%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILL 60
           MPSK RKALYAVKDQTSISLAKVSSS GG N  S+EVAILKATTHD+ P++ R+V EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSISLAKVSSSTGGPNAISLEVAILKATTHDDVPLEQRYVVEILH 60

Query: 61  LISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAK 120
           LISA+KS+AA CAH IAKRI++TR+WIVALKSLN+VL+IFQDGD +FPREVLH MKRGAK
Sbjct: 61  LISANKSHAAVCAHSIAKRINKTRHWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQKRFTQPKIQSYHLTTPRI 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTG LQKRFT+ K +S++  T RI
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTERKTESFNGNTRRI 180

Query: 181 NQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFDLYKDIS 240
           N+PI DMKPA LIDRI YWQKLLDRAIAT PTGPAKGNRLVL+S+HAVVQESFDLY+DIS
Sbjct: 181 NEPIRDMKPAMLIDRIYYWQKLLDRAIATRPTGPAKGNRLVLNSLHAVVQESFDLYRDIS 240

Query: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300
           DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS
Sbjct: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300

Query: 301 DELIETLQEFLKDQASFPCHGNRSLPLPPPSTAT----KYSD--EQSESS--------EI 360
           DELIETLQEFLKDQASFPCH +RS P P   + T    K +D  EQSESS        + 
Sbjct: 301 DELIETLQEFLKDQASFPCHDSRSPPAPVILSGTISTMKLTDDLEQSESSDKNSERELDF 360

Query: 361 NSLGDIMSGTGSW----NSIDHDGESVSDYQ---QSQMGDFANES----NFEFFDEIGKP 420
           NS G++MS TGSW    NS++ DGE  SDYQ   QS++GDFANES    NF FF++  KP
Sbjct: 361 NSFGEMMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANESNLSPNFAFFEDDEKP 420

Query: 421 --EETQF------RSGDWELILAESATEPPPEWPDFFGSIAGGDLFSKPF-SPQNHHSNP 480
             EE++F      R GDWEL+LAESAT+   EWPDFF    G D F KPF SPQ+ ++NP
Sbjct: 421 FQEESEFGAAPGLRCGDWELVLAESATDTAEEWPDFFAPSIGDDPFEKPFSSPQHQYNNP 480

Query: 481 FLQD---SFTIQP-----AEITPTFRAESIAIA-----------PTFKAVNQNGSVFGAW 540
           FLQD   SF + P      EI PTFRAE++  A           PTF + +QNGS+F  W
Sbjct: 481 FLQDSNESFAVPPTFKAETEIAPTFRAENVKEAVVVAPTFRVETPTFSSQHQNGSMFDDW 540

Query: 541 DPNESGSKQSVSGDPFGFEICTENDPFGDPWRSDLNGSDRSFEVVNQESLLEQQKLWKEQ 556
            P  +GS    SG  F F++  ENDPF + W S+LNG++ S  V++QESLL+QQKLWKEQ
Sbjct: 541 GPIPNGSG---SGSGFEFDLGAENDPFAEAWNSNLNGNEPSLNVMDQESLLQQQKLWKEQ 600

BLAST of Sed0010852 vs. ExPASy TrEMBL
Match: A0A6J1GKV6 (clathrin coat assembly protein AP180 OS=Cucurbita moschata OX=3662 GN=LOC111455292 PE=4 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 5.7e-218
Identity = 419/608 (68.91%), Postives = 475/608 (78.12%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILL 60
           MPSK RKALYAVKDQTSISLAKVSSS GG N  S+EVAILKATTHD+ P++ R+V+EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSISLAKVSSSTGGPNAISLEVAILKATTHDDVPLEQRYVAEILQ 60

Query: 61  LISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAK 120
           LISA+KS+AA CAH IAKRI++TR+WIVALKSLN+VL+IFQDGD +FPREVLH MKRGAK
Sbjct: 61  LISANKSHAAVCAHSIAKRINKTRHWIVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQKRFTQPKIQSYHLTTPRI 180
           ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTG LQKRFT+ K +S+H  T RI
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTERKTESFHGNTRRI 180

Query: 181 NQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFDLYKDIS 240
           N+PI DMKPA LIDRI YWQKLLDRAIAT PTGPAKGNRLVL+S+HAVVQESFDLY+DIS
Sbjct: 181 NEPIRDMKPAMLIDRIYYWQKLLDRAIATRPTGPAKGNRLVLNSLHAVVQESFDLYRDIS 240

Query: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300
           DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS
Sbjct: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300

Query: 301 DELIETLQEFLKDQASFPCHGNRSLPLPPPSTAT----KYSD--EQSESS--------EI 360
           DELIETLQEFLKDQASFPCH +RS P P   + T    K +D  EQSESS        + 
Sbjct: 301 DELIETLQEFLKDQASFPCHDSRSPPAPVILSGTISTMKLTDDLEQSESSDKNSERELDF 360

Query: 361 NSLGDIMSGTGSW----NSIDHDGESVSDYQ---QSQMGDFANES----NFEFFDEIGKP 420
           NS G++MS TGSW    NS++ +GE  SDYQ   QS++GDFANES    NF FF++   P
Sbjct: 361 NSFGEMMSATGSWTSPANSVEQEGEGYSDYQSEKQSRLGDFANESNLSPNFAFFEDDETP 420

Query: 421 --EETQ------FRSGDWELILAESATEPPPEWPDFFGSIAGGDLFSKPF-SPQNHHSNP 480
             EET+       R GDWEL+LAESAT+   EWPDFF    G D F KPF SPQ+ ++NP
Sbjct: 421 FQEETELGAARGLRCGDWELVLAESATDTAEEWPDFFAPSIGDDPFEKPFTSPQHQYNNP 480

Query: 481 FLQD---SFTIQP-----AEITPTFRA----ESIAIAPTFK-------AVNQNGSVFGAW 540
           FLQD   SF + P      EI PTFRA    E++A+APTF+       + NQNGS+F  W
Sbjct: 481 FLQDSNESFAVPPTFKAETEIAPTFRAENVKEAVAVAPTFRVETPTFSSQNQNGSMFDDW 540

Query: 541 DPNESGSKQSVSGDPFGFEICTENDPFGDPWRSDLNGSDRSFEVVNQESLLEQQKLWKEQ 556
            P  +G     SG  F F++  ENDPF + W S LNG++ S  V++QESLL+QQKLWKEQ
Sbjct: 541 GPIPNG-----SGSGFEFDLGAENDPFAEAWDSSLNGNEPSLNVMDQESLLQQQKLWKEQ 600

BLAST of Sed0010852 vs. TAIR 10
Match: AT1G05020.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 461.8 bits (1187), Expect = 7.6e-130
Identity = 309/670 (46.12%), Postives = 386/670 (57.61%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSG-GGTNRNSIEVAILKATTHDNS-PMDHRHVSEI 60
           MPSK +KA+ AVKDQTSISLAKV++   GG +  ++EVAILKAT+HD   P+D R V+EI
Sbjct: 1   MPSKLKKAIGAVKDQTSISLAKVANGATGGGDLTTLEVAILKATSHDEEVPIDDRLVTEI 60

Query: 61  LLLISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRG 120
           L +IS+ KS+AA+CA  I +RI RTRNWIVALKSL +VLRIFQDGD +FPREVLH MKRG
Sbjct: 61  LGIISSKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKRG 120

Query: 121 AKILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQKRFTQ----PKIQSYH 180
           AKILNLS+FRDDSNS PWD+TAFVRTFALYLDERLDCFLTG LQ+R+T      +I +  
Sbjct: 121 AKILNLSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKLQRRYTNREQTGRISTNS 180

Query: 181 LTTPRIN---------QPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSV 240
            T  R N           + DMKP  L+D+ITYWQKLLDRAIAT PTG AK NRLV  S+
Sbjct: 181 TTRSRFNPKAGIKSHEPAVRDMKPVMLLDKITYWQKLLDRAIATRPTGDAKANRLVKMSL 240

Query: 241 HAVVQESFDLYKDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSI 300
           +AV+QESFDLY+DISDGLALLLDSFFHLQYQSC+NAFQACV+A+KQFEEL +FYDL KSI
Sbjct: 241 YAVMQESFDLYRDISDGLALLLDSFFHLQYQSCINAFQACVRASKQFEELNAFYDLSKSI 300

Query: 301 GVGRTSEYPSVQQPSDELIETLQEFLKDQASFPCHG------NRSLPLPPPS-------- 360
           G+GRTSEYPS+Q+ S EL+ETLQEFLKDQ+SFP         N  LP PP S        
Sbjct: 301 GIGRTSEYPSIQKISLELLETLQEFLKDQSSFPASSGLYPSPNSFLPPPPSSKDSAVSSS 360

Query: 361 ---------TATKYSDEQSESSEINSLGDIMS----GTGS------------------WN 420
                    T+ +YSD  S  S   SL D+MS    GT S                   N
Sbjct: 361 LDFGDSTIDTSERYSDYGSFRS--TSLEDLMSRTEAGTSSPPMSCHSEPYGGGRDDPNGN 420

Query: 421 SID-------------------------HDGESVSDYQQSQMGDFANESNFEFFD----- 480
           + D                          D  + ++ +  +     ++S  E FD     
Sbjct: 421 NFDTVSTKSLPNNPSVSASNLILDLLSLDDVSNTAEAEDVEDKKKQDDSKAETFDPWEAL 480

Query: 481 ---------------EIGKPEETQFRSGDWELILAESATEPPPEWPDFFGSIA----GGD 540
                          E    E+ Q  SG+W L L E+AT+          S+A    G D
Sbjct: 481 MLRDDPKKKIETIEEEPSTAEDHQRDSGNWLLALEETATQVQGN-----NSMAIVPFGLD 540

Query: 541 LFSKPFSPQNHHSNPFLQDSFTIQPAEITPTFRAESIAIA-----PTFKAVNQNGSVFGA 557
                F       NPFL++          P      +A+      PTF+      +V   
Sbjct: 541 DPMPAFQAATDQYNPFLEEPVAQLATAGEPMITFGGLALTGFQPEPTFQV-----NVPDD 600

BLAST of Sed0010852 vs. TAIR 10
Match: AT4G32285.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 258.8 bits (660), Expect = 9.7e-69
Identity = 154/395 (38.99%), Postives = 232/395 (58.73%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILL 60
           M    RKA+  VKDQTSI +AKV+S+        +EVAI+KAT+HD+     +++ EIL 
Sbjct: 1   MALSMRKAIGVVKDQTSIGIAKVASN----MAPDLEVAIVKATSHDDDQSSDKYIREILS 60

Query: 61  LISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAK 120
           L S S+    AC   +++R+ +TR+WIVALK+L +V R+  +GD  F  E+L+  +RG +
Sbjct: 61  LTSLSRGYVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTR 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFL--------------------TGN 180
           ILN+S+FRD+++SS WD++AFVRT+A YLD+RL+  L                     G 
Sbjct: 121 ILNMSDFRDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGY 180

Query: 181 LQKR--FTQPKIQSYHLTT------PR-------INQ-----------PIPDMKPATLID 240
            + R  F  P  ++Y   T      P+       +N+           P+ +M P  +  
Sbjct: 181 NRSRDDFRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFG 240

Query: 241 RITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFDLYKDISDGLALLLDSFFHLQ 300
           ++ + Q+LLDR ++  PTG AK +R++L +++ VV+ESF LY DI + LA+LLD FF ++
Sbjct: 241 KMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDME 300

Query: 301 YQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPSDELIETLQEFLKDQ 350
           Y  CV AF A   AAKQ +EL +FY  CK  GV R+SEYP VQ+ + +L+ETL+EF++D+
Sbjct: 301 YTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDR 360

BLAST of Sed0010852 vs. TAIR 10
Match: AT4G32285.2 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 258.8 bits (660), Expect = 9.7e-69
Identity = 154/395 (38.99%), Postives = 232/395 (58.73%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILL 60
           M    RKA+  VKDQTSI +AKV+S+        +EVAI+KAT+HD+     +++ EIL 
Sbjct: 1   MALSMRKAIGVVKDQTSIGIAKVASN----MAPDLEVAIVKATSHDDDQSSDKYIREILS 60

Query: 61  LISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAK 120
           L S S+    AC   +++R+ +TR+WIVALK+L +V R+  +GD  F  E+L+  +RG +
Sbjct: 61  LTSLSRGYVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTR 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFL--------------------TGN 180
           ILN+S+FRD+++SS WD++AFVRT+A YLD+RL+  L                     G 
Sbjct: 121 ILNMSDFRDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGY 180

Query: 181 LQKR--FTQPKIQSYHLTT------PR-------INQ-----------PIPDMKPATLID 240
            + R  F  P  ++Y   T      P+       +N+           P+ +M P  +  
Sbjct: 181 NRSRDDFRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFG 240

Query: 241 RITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFDLYKDISDGLALLLDSFFHLQ 300
           ++ + Q+LLDR ++  PTG AK +R++L +++ VV+ESF LY DI + LA+LLD FF ++
Sbjct: 241 KMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDME 300

Query: 301 YQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPSDELIETLQEFLKDQ 350
           Y  CV AF A   AAKQ +EL +FY  CK  GV R+SEYP VQ+ + +L+ETL+EF++D+
Sbjct: 301 YTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDR 360

BLAST of Sed0010852 vs. TAIR 10
Match: AT2G25430.1 (epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related )

HSP 1 Score: 256.9 bits (655), Expect = 3.7e-68
Identity = 151/416 (36.30%), Postives = 232/416 (55.77%), Query Frame = 0

Query: 1   MPSKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILL 60
           M    RKA+ AVKDQTSI +AKV+S+        +EVAI+KAT+HD+ P   +++ EIL 
Sbjct: 1   MAPSIRKAIGAVKDQTSIGIAKVASN----MAPDLEVAIVKATSHDDDPASEKYIREILN 60

Query: 61  LISASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAK 120
           L S S+    AC   +++R+S+TR+W+VALK+L +V R+  +GD  F  E+L+  +RG +
Sbjct: 61  LTSLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTR 120

Query: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFL----------------------- 180
           +LN+S+FRD+++SS WD++AFVRT+A YLD+RL+  L                       
Sbjct: 121 MLNMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDD 180

Query: 181 -TGNLQKRFTQPKIQSYHLTT--------------------------------------- 240
             G  +  F  P  +SY                                           
Sbjct: 181 RYGRGRDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGGG 240

Query: 241 ---PRINQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLHSVHAVVQESFD 300
               ++  P+ +M P  +  ++ + Q+LLDR ++  PTG AK +R++L +++ VV+ESF 
Sbjct: 241 RDEKKVVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFK 300

Query: 301 LYKDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYP 350
           LY DI + LA+LLD FF ++Y  CV AF A   AAKQ +EL +FY+ CK  GV R+SEYP
Sbjct: 301 LYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYP 360

BLAST of Sed0010852 vs. TAIR 10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 9.1e-67
Identity = 132/371 (35.58%), Postives = 225/371 (60.65%), Query Frame = 0

Query: 3   SKFRKALYAVKDQTSISLAKVSSSGGGTNRNSIEVAILKATTHDNSPMDHRHVSEILLLI 62
           SK ++A+ AVKDQTS+ LAKV   G  ++   +E+A++KAT HD+ P + +++ EIL L 
Sbjct: 4   SKLKRAIGAVKDQTSVGLAKV--GGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCLT 63

Query: 63  SASKSNAAACAHYIAKRISRTRNWIVALKSLNIVLRIFQDGDLHFPREVLHVMKRGAKIL 122
           S S++  +AC   +++R+++T+NW VALK+L ++ R+  DGD  + +E+    +RG ++L
Sbjct: 64  SYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRLL 123

Query: 123 NLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGNLQK------------------- 182
           N+S+FRD S S  WDY+AFVRT+ALYLDERLD  + G   K                   
Sbjct: 124 NMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEEDDH 183

Query: 183 RFTQPKIQSYHLTTPRINQPIPDMKPATLIDRITYWQKLLDRAIATTPTGPAKGNRLVLH 242
           R T   I+S  +     ++P+ +MK   + +R+ + Q+LLDR +A  PTG AK NR+V+ 
Sbjct: 184 RGTSNDIRSKAIVVK--SKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIV 243

Query: 243 SVHAVVQESFDLYKDISDGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCK 302
           +++ +V+ESF LY +I++ + +L++ F  L     +  ++   + +KQF+EL  FY  CK
Sbjct: 244 AMYPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCK 303

Query: 303 SIGVGRTSEYPSVQQPSDELIETLQEFLKDQASFPCH-----GNRSLPLPPPSTATKYSD 350
           ++ V R+SEYP +++ + + ++ + EF++D+++           RS       + T+Y  
Sbjct: 304 NMAVARSSEYPELEKITQKKLDLMDEFIRDKSALAAQTTKSSSKRSNKSEEEESKTEYIQ 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038887470.14.3e-22871.66clathrin coat assembly protein AP180 [Benincasa hispida][more]
XP_008449479.19.7e-22071.97PREDICTED: clathrin coat assembly protein AP180 [Cucumis melo] >KAA0061650.1 cla... [more]
XP_004140244.12.2e-21971.45clathrin coat assembly protein AP180 [Cucumis sativus] >KGN47964.1 hypothetical ... [more]
XP_023553928.16.9e-21868.91clathrin coat assembly protein AP180-like [Cucurbita pepo subsp. pepo][more]
KAG6572416.19.0e-21868.91Clathrin coat assembly protein 180, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
Q9ZVN61.1e-12846.12Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE... [more]
Q8S9J81.4e-6738.99Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8LF205.2e-6736.30Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8GX471.3e-6535.58Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9SA656.4e-6536.13Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A5D3C6854.7e-22071.97Clathrin coat assembly protein AP180 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BN174.7e-22071.97clathrin coat assembly protein AP180 OS=Cucumis melo OX=3656 GN=LOC103491352 PE=... [more]
A0A0A0KGJ51.0e-21971.45ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G421580 PE=4 S... [more]
A0A6J1I2N84.4e-21868.91clathrin coat assembly protein AP180-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1GKV65.7e-21868.91clathrin coat assembly protein AP180 OS=Cucurbita moschata OX=3662 GN=LOC1114552... [more]
Match NameE-valueIdentityDescription
AT1G05020.17.6e-13046.12ENTH/ANTH/VHS superfamily protein [more]
AT4G32285.19.7e-6938.99ENTH/ANTH/VHS superfamily protein [more]
AT4G32285.29.7e-6938.99ENTH/ANTH/VHS superfamily protein [more]
AT2G25430.13.7e-6836.30epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly p... [more]
AT4G02650.19.1e-6735.58ENTH/ANTH/VHS superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013809ENTH domainSMARTSM00273enth_2coord: 33..163
e-value: 1.8E-37
score: 140.5
IPR013809ENTH domainPROSITEPS50942ENTHcoord: 27..163
score: 26.892519
IPR008942ENTH/VHSGENE3D1.25.40.90coord: 2..160
e-value: 7.2E-37
score: 128.3
IPR008942ENTH/VHSSUPERFAMILY48464ENTH/VHS domaincoord: 33..155
IPR014712ANTH domain superfamilyGENE3D1.20.58.150ANTH domaincoord: 178..319
e-value: 8.9E-32
score: 111.7
IPR011417AP180 N-terminal homology (ANTH) domainPFAMPF07651ANTHcoord: 35..313
e-value: 6.3E-78
score: 261.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 321..343
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 327..343
NoneNo IPR availablePANTHERPTHR22951:SF75CLATHRIN COAT ASSEMBLY PROTEIN AP180coord: 1..555
NoneNo IPR availablePANTHERPTHR22951CLATHRIN ASSEMBLY PROTEINcoord: 1..555
NoneNo IPR availableCDDcd16987ANTH_N_AP180_plantcoord: 35..156
e-value: 3.97832E-57
score: 185.135
NoneNo IPR availableSUPERFAMILY89009GAT-like domaincoord: 184..310

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0010852.1Sed0010852.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048268 clathrin coat assembly
biological_process GO:0072583 clathrin-dependent endocytosis
biological_process GO:0006900 vesicle budding from membrane
cellular_component GO:0005905 clathrin-coated pit
cellular_component GO:0030136 clathrin-coated vesicle
cellular_component GO:0005794 Golgi apparatus
molecular_function GO:0005545 1-phosphatidylinositol binding
molecular_function GO:0032050 clathrin heavy chain binding
molecular_function GO:0005546 phosphatidylinositol-4,5-bisphosphate binding
molecular_function GO:0000149 SNARE binding
molecular_function GO:0030276 clathrin binding
molecular_function GO:0005543 phospholipid binding