Sed0010583 (gene) Chayote v1

Overview
NameSed0010583
Typegene
OrganismSechium edule (Chayote v1)
Descriptioncytochrome P450 94B3
LocationLG08: 169980 .. 172206 (+)
RNA-Seq ExpressionSed0010583
SyntenySed0010583
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGGAGAAGAGAAAAATAAATTAGCCACGTTTATCAAAAACGGCGTCTTTGTCCTATTAACTAAAAAAATCCTTTGAAAAATGGCTACTTTTGCATACACGTCAGTGGCTCTCCACGGTTGTCAATTTCCCACGTGGTCTTTGTTTCACAGCTCACTTGATTTCCCCACCCTATAAATAGTAAAATAAAGCTCTCTCAAAAATATTCAACAACCATGCAGCTCAACTTCGGCATCTCCCTCTTCTTCCTTCTCTGTCTTTCTTTATGTGCTTTCTTTTTTCTTTACCCTTTCATCAGCTGCTTGGTTTCTTTCCACAAAAACCGGCGCCGGCTTCTTAGCTGGTACACCGACCTGCTTTCCGACTCTTCGACCCGGACCATTGTGGTGCACCGGCTCGGTTCGAGGCGGACCATTGTCACGGCCAACCCAGCCAACGTTGAATACATGCTGAAGACTAATTTTGTTAATTACCCTAAAGGGAAGCCGTTCACTGATATACTCGGCGATCTCCTCGGGTGTGGCATTTTCAACGTCGACGGGGACTTGTGGTCAACGCAGCGCAAGCTCGCCAGTCACGAATTTTCTGCTAAGTCTTTGAGGGAATTTGTCGTTAAGATTCTCGAAGACGAAGTTCATCTCCGTTTGATTCCGTTGCTCCAGAATGCTGCCCGGACTAACGGCGTCTTGGATTTGCAAGATGTTCTGGGAAGGCTTGCTTTTGACACCATTTGTAAGGTTTGTCTTATAATAACCTTCTTCAAAAGTATAGATCAGGTTAAATCCTTAATTACCCACTTAAATATATATATATATATATATATATATATTTTTTGTATTTTTTTAGTTTAGCAGTAGAATAATTCAAATCGTATAATGTTTCTATCAAATATAGAAGTTCATTATTGTGTTTGAACTATATATAAATATATATTCTGGTTTGACAGACAATAATATTTTGATACTATGACAGGTTACACTAATATTTATTTGATGTTTGAATTTGCATCCGACCAGTTGGGATATAAGTTGGTTAATGTTGGTGTGGTTGTGCGTTGTAAACAGGTGAGTCTAGGAAGTGACGAGCAATGTCTGGACATGTCGCGCCCAGTTCCAGCAATACTAAATGCATTCGACACCGCCACGGGGATCAGCGCCAAGCGTGCAATAGCGCCAATCTTTCTGGCGTGGAAAGCCAAGCGCCTCTTCAACATCGGCTCCGAAAAGCAGCTCAAAGAATCGGTCAGAAGGGTCCACTCATGGGTCTCCGACATCATTCGCAACAAAAACCTCGACACCAACAAACCCGCGGGAGACTTGTTGTCTCGGCTGTTATGCGCGGGCAACGACCAAGAAGTTGTCCGAGACATGGTAGTCAGCTTCATAATGGCCGGCCGCGACACCACCTCCGCTGCCATGACGTGGCTCTTCTGGTTGCTTACCAAACACCCCGAAGTCCAAGCCACCCTAATGAACGAAGTGGTTTCCTTGTCTAACAAAACTGCGTCGTTTAATTATGGATATGAAGAATTGAAGGACATGAAGTATTTGAAGGCGTGTTTGTGTGAGTCCATGAGGCTTTACCCGCCCGTGGCTTGGGACTCCAAGCACGCGGCGGCACCCGACGTTCTTCCAGATGGAACTCGGGTGAGGAAAGGGGACAGAGTCACGTATTTTCCCTACGGTATGGGACGGATGGAGGACCTGTGGGGGAAGGACCGGCTCGAATTCAAACCGGACCGGTGGTTCCAAAACGGGGAGTTGAAACCGGTCAGTCCTTTCAAGTTTCCGGTTTTTCAAGCCGGTCCGAGGATGTGCTTGGGAAAGGAAATGGCGTTCATCCAGATGAAATACGTGGTGGCCACGGTTTTGAAGCGGTTTGAATTTAGACCGGTCAGTGAAAGTCCACCAGTATTTGTCCCTTTATTGACAGCTCATATGGCTGGTGGATTACAGGTTTATGTTAGAGAAAGGACAGGACAAAAGTCAACCGTCTGGTGATGATCAAATCGTGTGTGTTTTCTTTTTCTTTTTTTTCCCCCCCTTTTTTTAAGTACAAAATCGTGTGTGTTTTCAGTTGGCAGTAATCGACGTATCGCGCCGCAAACTCAACATTTAATTAGTATATTAAACATATAAAATGAATGGTTAAGTATTCCAAATTCCAATCCAAGTCGGTTCTATGTTTTTTTTCGCCAAAACTAATTTCTTGTATGAATATTTGACAGC

mRNA sequence

GTGGAGAAGAGAAAAATAAATTAGCCACGTTTATCAAAAACGGCGTCTTTGTCCTATTAACTAAAAAAATCCTTTGAAAAATGGCTACTTTTGCATACACGTCAGTGGCTCTCCACGGTTGTCAATTTCCCACGTGGTCTTTGTTTCACAGCTCACTTGATTTCCCCACCCTATAAATAGTAAAATAAAGCTCTCTCAAAAATATTCAACAACCATGCAGCTCAACTTCGGCATCTCCCTCTTCTTCCTTCTCTGTCTTTCTTTATGTGCTTTCTTTTTTCTTTACCCTTTCATCAGCTGCTTGGTTTCTTTCCACAAAAACCGGCGCCGGCTTCTTAGCTGGTACACCGACCTGCTTTCCGACTCTTCGACCCGGACCATTGTGGTGCACCGGCTCGGTTCGAGGCGGACCATTGTCACGGCCAACCCAGCCAACGTTGAATACATGCTGAAGACTAATTTTGTTAATTACCCTAAAGGGAAGCCGTTCACTGATATACTCGGCGATCTCCTCGGGTGTGGCATTTTCAACGTCGACGGGGACTTGTGGTCAACGCAGCGCAAGCTCGCCAGTCACGAATTTTCTGCTAAGTCTTTGAGGGAATTTGTCGTTAAGATTCTCGAAGACGAAGTTCATCTCCGTTTGATTCCGTTGCTCCAGAATGCTGCCCGGACTAACGGCGTCTTGGATTTGCAAGATGTTCTGGGAAGGCTTGCTTTTGACACCATTTGTAAGGTGAGTCTAGGAAGTGACGAGCAATGTCTGGACATGTCGCGCCCAGTTCCAGCAATACTAAATGCATTCGACACCGCCACGGGGATCAGCGCCAAGCGTGCAATAGCGCCAATCTTTCTGGCGTGGAAAGCCAAGCGCCTCTTCAACATCGGCTCCGAAAAGCAGCTCAAAGAATCGGTCAGAAGGGTCCACTCATGGGTCTCCGACATCATTCGCAACAAAAACCTCGACACCAACAAACCCGCGGGAGACTTGTTGTCTCGGCTGTTATGCGCGGGCAACGACCAAGAAGTTGTCCGAGACATGGTAGTCAGCTTCATAATGGCCGGCCGCGACACCACCTCCGCTGCCATGACGTGGCTCTTCTGGTTGCTTACCAAACACCCCGAAGTCCAAGCCACCCTAATGAACGAAGTGGTTTCCTTGTCTAACAAAACTGCGTCGTTTAATTATGGATATGAAGAATTGAAGGACATGAAGTATTTGAAGGCGTGTTTGTGTGAGTCCATGAGGCTTTACCCGCCCGTGGCTTGGGACTCCAAGCACGCGGCGGCACCCGACGTTCTTCCAGATGGAACTCGGGTGAGGAAAGGGGACAGAGTCACGTATTTTCCCTACGGTATGGGACGGATGGAGGACCTGTGGGGGAAGGACCGGCTCGAATTCAAACCGGACCGGTGGTTCCAAAACGGGGAGTTGAAACCGGTCAGTCCTTTCAAGTTTCCGGTTTTTCAAGCCGGTCCGAGGATGTGCTTGGGAAAGGAAATGGCGTTCATCCAGATGAAATACGTGGTGGCCACGGTTTTGAAGCGGTTTGAATTTAGACCGGTCAGTGAAAGTCCACCAGTATTTGTCCCTTTATTGACAGCTCATATGGCTGGTGGATTACAGGTTTATGTTAGAGAAAGGACAGGACAAAAGTCAACCGTCTGGTGATGATCAAATCGTGTGTGTTTTCTTTTTCTTTTTTTTCCCCCCCTTTTTTTAAGTACAAAATCGTGTGTGTTTTCAGTTGGCAGTAATCGACGTATCGCGCCGCAAACTCAACATTTAATTAGTATATTAAACATATAAAATGAATGGTTAAGTATTCCAAATTCCAATCCAAGTCGGTTCTATGTTTTTTTTCGCCAAAACTAATTTCTTGTATGAATATTTGACAGC

Coding sequence (CDS)

ATGCAGCTCAACTTCGGCATCTCCCTCTTCTTCCTTCTCTGTCTTTCTTTATGTGCTTTCTTTTTTCTTTACCCTTTCATCAGCTGCTTGGTTTCTTTCCACAAAAACCGGCGCCGGCTTCTTAGCTGGTACACCGACCTGCTTTCCGACTCTTCGACCCGGACCATTGTGGTGCACCGGCTCGGTTCGAGGCGGACCATTGTCACGGCCAACCCAGCCAACGTTGAATACATGCTGAAGACTAATTTTGTTAATTACCCTAAAGGGAAGCCGTTCACTGATATACTCGGCGATCTCCTCGGGTGTGGCATTTTCAACGTCGACGGGGACTTGTGGTCAACGCAGCGCAAGCTCGCCAGTCACGAATTTTCTGCTAAGTCTTTGAGGGAATTTGTCGTTAAGATTCTCGAAGACGAAGTTCATCTCCGTTTGATTCCGTTGCTCCAGAATGCTGCCCGGACTAACGGCGTCTTGGATTTGCAAGATGTTCTGGGAAGGCTTGCTTTTGACACCATTTGTAAGGTGAGTCTAGGAAGTGACGAGCAATGTCTGGACATGTCGCGCCCAGTTCCAGCAATACTAAATGCATTCGACACCGCCACGGGGATCAGCGCCAAGCGTGCAATAGCGCCAATCTTTCTGGCGTGGAAAGCCAAGCGCCTCTTCAACATCGGCTCCGAAAAGCAGCTCAAAGAATCGGTCAGAAGGGTCCACTCATGGGTCTCCGACATCATTCGCAACAAAAACCTCGACACCAACAAACCCGCGGGAGACTTGTTGTCTCGGCTGTTATGCGCGGGCAACGACCAAGAAGTTGTCCGAGACATGGTAGTCAGCTTCATAATGGCCGGCCGCGACACCACCTCCGCTGCCATGACGTGGCTCTTCTGGTTGCTTACCAAACACCCCGAAGTCCAAGCCACCCTAATGAACGAAGTGGTTTCCTTGTCTAACAAAACTGCGTCGTTTAATTATGGATATGAAGAATTGAAGGACATGAAGTATTTGAAGGCGTGTTTGTGTGAGTCCATGAGGCTTTACCCGCCCGTGGCTTGGGACTCCAAGCACGCGGCGGCACCCGACGTTCTTCCAGATGGAACTCGGGTGAGGAAAGGGGACAGAGTCACGTATTTTCCCTACGGTATGGGACGGATGGAGGACCTGTGGGGGAAGGACCGGCTCGAATTCAAACCGGACCGGTGGTTCCAAAACGGGGAGTTGAAACCGGTCAGTCCTTTCAAGTTTCCGGTTTTTCAAGCCGGTCCGAGGATGTGCTTGGGAAAGGAAATGGCGTTCATCCAGATGAAATACGTGGTGGCCACGGTTTTGAAGCGGTTTGAATTTAGACCGGTCAGTGAAAGTCCACCAGTATTTGTCCCTTTATTGACAGCTCATATGGCTGGTGGATTACAGGTTTATGTTAGAGAAAGGACAGGACAAAAGTCAACCGTCTGGTGA

Protein sequence

MQLNFGISLFFLLCLSLCAFFFLYPFISCLVSFHKNRRRLLSWYTDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNFVNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLRLIPLLQNAARTNGVLDLQDVLGRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGISAKRAIAPIFLAWKAKRLFNIGSEKQLKESVRRVHSWVSDIIRNKNLDTNKPAGDLLSRLLCAGNDQEVVRDMVVSFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLSNKTASFNYGYEELKDMKYLKACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYFPYGMGRMEDLWGKDRLEFKPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFEFRPVSESPPVFVPLLTAHMAGGLQVYVRERTGQKSTVW
Homology
BLAST of Sed0010583 vs. NCBI nr
Match: XP_038879988.1 (cytochrome P450 94B3-like [Benincasa hispida])

HSP 1 Score: 820.5 bits (2118), Expect = 7.8e-234
Identity = 407/513 (79.34%), Postives = 445/513 (86.74%), Query Frame = 0

Query: 5   FGISLFFLLCLSLCAFFFL---------------------YPFISCLVSFHKNRRRLLSW 64
           F +SLF LL  SL AFFFL                     YPFI CL+SF+KNRRRLL+W
Sbjct: 4   FTMSLFLLLSTSLWAFFFLYLCIFKLSCFSRKRFYYSPPSYPFIGCLISFYKNRRRLLAW 63

Query: 65  YTDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNFVNYPKGKPFTDILGDLLGCG 124
           YTDLLSDS T+TIV+ RLGSRRTIVTANPANVE+MLKTNF+NYPKGKPFTDILGDLLGCG
Sbjct: 64  YTDLLSDSPTQTIVMQRLGSRRTIVTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCG 123

Query: 125 IFNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLRLIPLLQNAARTNGVLDLQDV 184
           IFNVDGDLWS+QRKLASH FSAKSLREFVVK LEDEVHLRLIPLLQNAA+TN VLDLQDV
Sbjct: 124 IFNVDGDLWSSQRKLASHAFSAKSLREFVVKTLEDEVHLRLIPLLQNAAQTNAVLDLQDV 183

Query: 185 LGRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGISAKRAIAPIFLAWKAKRLFN 244
           LGRLAFDT+CKV+LG+DEQCLDMSRP+P I+NAFDTATG+SAKRAIAP+FL WKAKR+ N
Sbjct: 184 LGRLAFDTVCKVTLGTDEQCLDMSRPIPEIVNAFDTATGVSAKRAIAPLFLTWKAKRMLN 243

Query: 245 IGSEKQLKESVRRVHSWVSDIIRNKNLDTNKPA---------GDLLSRLLCAGNDQEVVR 304
           +GSEK+LKE+V+ VH WVS+IIRNKNL+ N             DLLSRL  AG+D+EVVR
Sbjct: 244 LGSEKKLKEAVQMVHKWVSNIIRNKNLNNNNNTLPNHNQTHNNDLLSRLQSAGHDEEVVR 303

Query: 305 DMVVSFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLSN--KTASFNYGYEELKD 364
           DM++SFIMAGRDTTSAAMTWLFWLLTKHPEV+  ++NE  SLS+  K+ S  YGYEELKD
Sbjct: 304 DMIISFIMAGRDTTSAAMTWLFWLLTKHPEVEEAIINEASSLSDDLKSTSLGYGYEELKD 363

Query: 365 MKYLKACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYFPYGMGRMEDLWGKD 424
           MKYLKACLCESMRLYPPVAWDSKHAAAPD+LPDGT VRKGDRVTYFPYGMGRME+LWGKD
Sbjct: 364 MKYLKACLCESMRLYPPVAWDSKHAAAPDILPDGTPVRKGDRVTYFPYGMGRMEELWGKD 423

Query: 425 RLEFKPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFEFRPVS 484
           RLEFKPDRW+QNGELK VSPFKFPVFQAGPRMCLG EMAFIQMKYVVATVLKRFEFRP S
Sbjct: 424 RLEFKPDRWYQNGELKTVSPFKFPVFQAGPRMCLGMEMAFIQMKYVVATVLKRFEFRPAS 483

Query: 485 ESPPVFVPLLTAHMAGGLQVYVRERTGQKSTVW 486
           E+ PVFVPLLTAHMAGGLQVYVRERT QKST+W
Sbjct: 484 ENVPVFVPLLTAHMAGGLQVYVRERTEQKSTIW 516

BLAST of Sed0010583 vs. NCBI nr
Match: XP_022966357.1 (cytochrome P450 94B1-like [Cucurbita maxima])

HSP 1 Score: 816.6 bits (2108), Expect = 1.1e-232
Identity = 409/499 (81.96%), Postives = 441/499 (88.38%), Query Frame = 0

Query: 4   NFGISLFFLLCLSLCAFFFL-------------------YPFISCLVSFHKNRRRLLSWY 63
           +  +SLF LL +SL AFF +                   YPFI C +SF KNRRRLLSWY
Sbjct: 3   DLAVSLFLLLSISLWAFFLIYVFIYKFHRFSTNRSSPPSYPFIGCFISFFKNRRRLLSWY 62

Query: 64  TDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNFVNYPKGKPFTDILGDLLGCGI 123
           T LLS+S + TIVVHRLGSRRT+VTANPANVEYMLKTNF+NYPKGKPFTDILGDLLGCGI
Sbjct: 63  THLLSNSPSLTIVVHRLGSRRTVVTANPANVEYMLKTNFLNYPKGKPFTDILGDLLGCGI 122

Query: 124 FNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLRLIPLLQNAARTNGVLDLQDVL 183
           FNVDG+LWSTQRKLASHEFSAKSLREFVVK LEDE+HLRLIPLLQNAARTNGVLDLQDVL
Sbjct: 123 FNVDGELWSTQRKLASHEFSAKSLREFVVKTLEDEIHLRLIPLLQNAARTNGVLDLQDVL 182

Query: 184 GRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGISAKRAIAPIFLAWKAKRLFNI 243
           GRLAFDT+CK++LG+DEQCLDMSRPVPAI+NAFD ATGISAKRAIAP+ LAWKAKRLFNI
Sbjct: 183 GRLAFDTVCKMTLGTDEQCLDMSRPVPAIVNAFDMATGISAKRAIAPVSLAWKAKRLFNI 242

Query: 244 GSEKQLKESVRRVHSWVSDIIRNKNLDTNKPAGDLLSRLLCAGNDQEVVRDMVVSFIMAG 303
           GSEK+LKE+V+ VHSWVS IIRNKNLD N+ + DLLSRLL AG+ +EVVRDMVVSFIMAG
Sbjct: 243 GSEKRLKEAVQMVHSWVSHIIRNKNLDNNEDS-DLLSRLLSAGHGEEVVRDMVVSFIMAG 302

Query: 304 RDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLSN--KTASFNYGYEELKDMKYLKACLCE 363
           RDTTSAAMTWLFWLLTKHP+V+ TLM+EV SLSN  +TAS  YGYEELKDMKYLKACLCE
Sbjct: 303 RDTTSAAMTWLFWLLTKHPKVEQTLMDEVASLSNNYQTASLGYGYEELKDMKYLKACLCE 362

Query: 364 SMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYFPYGMGRMEDLWGKDRLEFKPDRWF 423
           SMRLYPPVAWDSKHAAA D+LPDGT VRKGDRVTYFPYGMGRME+LWG DR EFKP+RWF
Sbjct: 363 SMRLYPPVAWDSKHAAAADILPDGTPVRKGDRVTYFPYGMGRMEELWGPDREEFKPERWF 422

Query: 424 QNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFEFRPVSESPPVFVPLL 482
           + GE K VSPFKFPVFQ GPRMCLGKEMAFIQMKYVVATVLKRFEFR VSES PVFVPLL
Sbjct: 423 EKGEAKAVSPFKFPVFQGGPRMCLGKEMAFIQMKYVVATVLKRFEFRAVSESEPVFVPLL 482

BLAST of Sed0010583 vs. NCBI nr
Match: XP_022921449.1 (cytochrome P450 94B3 [Cucurbita moschata])

HSP 1 Score: 813.1 bits (2099), Expect = 1.3e-231
Identity = 402/508 (79.13%), Postives = 444/508 (87.40%), Query Frame = 0

Query: 5   FGISLFFLLCLSLCAFFFL--------------------YPFISCLVSFHKNRRRLLSWY 64
           F +SLFFLL LS  AFF L                    YPFI CL+SF+KNRRRLLSWY
Sbjct: 6   FALSLFFLLSLSSTAFFCLYLSFFRFYCFSKNRFYSPPSYPFIGCLISFYKNRRRLLSWY 65

Query: 65  TDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNFVNYPKGKPFTDILGDLLGCGI 124
           T LLS S T+TIVV+RLGSRRT+VTANPANVE+MLKTNFVNYPKGKPFTDILGDLLGCGI
Sbjct: 66  THLLSHSPTQTIVVNRLGSRRTVVTANPANVEHMLKTNFVNYPKGKPFTDILGDLLGCGI 125

Query: 125 FNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLRLIPLLQNAARTNGVLDLQDVL 184
           FNVDGDLWSTQRKLASHEFSAKS+REFVVK LEDEVHLRLIPLL NAARTN VLDLQDVL
Sbjct: 126 FNVDGDLWSTQRKLASHEFSAKSVREFVVKTLEDEVHLRLIPLLHNAARTNAVLDLQDVL 185

Query: 185 GRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGISAKRAIAPIFLAWKAKRLFNI 244
           GRLAFDT+CKV++G+DEQCLDMSRP+PAI+N+ DTATGISA+RA+AP+F+ WKAKR+ N+
Sbjct: 186 GRLAFDTVCKVTVGTDEQCLDMSRPIPAIVNSLDTATGISARRAVAPLFVTWKAKRMLNV 245

Query: 245 GSEKQLKESVRRVHSWVSDIIRNKN------LDTNKPAGDLLSRLLCAGNDQEVVRDMVV 304
           GSEK+LKE+V  VH WVS+IIRN+N      LD N   GDLLSRLL AG+ QEV+RDMVV
Sbjct: 246 GSEKKLKEAVEMVHEWVSNIIRNRNLNNSNTLDHNHAHGDLLSRLLSAGHSQEVIRDMVV 305

Query: 305 SFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLS--NKTASFNYGYEELKDMKYL 364
           SFIMAGRDTTSAAMTWLFWLLTKHP+V+  +++E + LS  ++TAS  YGYEELKDMKYL
Sbjct: 306 SFIMAGRDTTSAAMTWLFWLLTKHPKVEQAIVDEAIKLSGDSETASLGYGYEELKDMKYL 365

Query: 365 KACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYFPYGMGRMEDLWGKDRLEF 424
           KACLCESMRLYPPVAWDSKHAAAPD+LPDGT VRKGDRVTYFPYGMGRME+LWGKDRLEF
Sbjct: 366 KACLCESMRLYPPVAWDSKHAAAPDILPDGTPVRKGDRVTYFPYGMGRMEELWGKDRLEF 425

Query: 425 KPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFEFRPVSESPP 484
           KPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYV+ATVLKRFEFRP +E+ P
Sbjct: 426 KPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVMATVLKRFEFRPANENAP 485

BLAST of Sed0010583 vs. NCBI nr
Match: XP_022987517.1 (cytochrome P450 94B3-like [Cucurbita maxima])

HSP 1 Score: 812.0 bits (2096), Expect = 2.8e-231
Identity = 404/508 (79.53%), Postives = 443/508 (87.20%), Query Frame = 0

Query: 5   FGISLFFLLCLSLCAFFFL--------------------YPFISCLVSFHKNRRRLLSWY 64
           F + LFFLL LS  AFF L                    YPFI CL+SF+KNRRRLLSWY
Sbjct: 6   FALFLFFLLSLSSTAFFCLYLSFFKFYCFSKNLFYSPPSYPFIGCLISFYKNRRRLLSWY 65

Query: 65  TDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNFVNYPKGKPFTDILGDLLGCGI 124
           T LLS S T+TIVV+RLGSRRT+VTANPANVE+MLKTNFVNYPKGKPFTDILGDLLGCGI
Sbjct: 66  THLLSHSPTQTIVVNRLGSRRTVVTANPANVEHMLKTNFVNYPKGKPFTDILGDLLGCGI 125

Query: 125 FNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLRLIPLLQNAARTNGVLDLQDVL 184
           FNVDGDLWSTQRKLASHEFSAKS+REFVVK LEDEVHLRLIPLLQNAARTN VLDLQDVL
Sbjct: 126 FNVDGDLWSTQRKLASHEFSAKSVREFVVKTLEDEVHLRLIPLLQNAARTNAVLDLQDVL 185

Query: 185 GRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGISAKRAIAPIFLAWKAKRLFNI 244
           GRLAFDT+CKV++G+DEQCLDMSRP+PAI+N+ DTATGISA RA+AP+FL WKAKR+ N+
Sbjct: 186 GRLAFDTVCKVTVGTDEQCLDMSRPLPAIVNSLDTATGISAGRAVAPLFLTWKAKRMLNV 245

Query: 245 GSEKQLKESVRRVHSWVSDIIRNKN------LDTNKPAGDLLSRLLCAGNDQEVVRDMVV 304
           GSEK+LKE+V  VH WVS+IIRN+N      LD N   GDLLSRLL AG+ QEVVRDMVV
Sbjct: 246 GSEKKLKEAVEMVHEWVSNIIRNRNLNNSNTLDHNHTHGDLLSRLLSAGHSQEVVRDMVV 305

Query: 305 SFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLS--NKTASFNYGYEELKDMKYL 364
           SFIMAGRDTTSAAMTWLFWLLTKHP+V+  +++E + LS  ++TAS  YGYEELKDMKYL
Sbjct: 306 SFIMAGRDTTSAAMTWLFWLLTKHPKVEQVIVDEAIKLSGDSETASLGYGYEELKDMKYL 365

Query: 365 KACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYFPYGMGRMEDLWGKDRLEF 424
           KACLCESMRLYPPVAWDSKHAAAPD+LPDGT VRKGDRVTYFPYGMGRME+LWGKDRLEF
Sbjct: 366 KACLCESMRLYPPVAWDSKHAAAPDILPDGTPVRKGDRVTYFPYGMGRMEELWGKDRLEF 425

Query: 425 KPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFEFRPVSESPP 484
           KPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYV+ATVLKRFEFRP +E+ P
Sbjct: 426 KPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVMATVLKRFEFRPANENAP 485

BLAST of Sed0010583 vs. NCBI nr
Match: XP_023516482.1 (cytochrome P450 94B3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 809.7 bits (2090), Expect = 1.4e-230
Identity = 402/508 (79.13%), Postives = 442/508 (87.01%), Query Frame = 0

Query: 5   FGISLFFLLCLSLCAFFFL--------------------YPFISCLVSFHKNRRRLLSWY 64
           F +SLFFLL LS  AFF L                    YP I CL+SF+KNRRRLLSWY
Sbjct: 6   FALSLFFLLSLSSTAFFCLYLSFFKFYCFSKNRFYSPPSYPLIGCLISFYKNRRRLLSWY 65

Query: 65  TDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNFVNYPKGKPFTDILGDLLGCGI 124
           T LLS S T+TIVV+RLGSRRT+VTANPANVE+MLKTNFVNYPKGKPFTDILGDLLGCGI
Sbjct: 66  THLLSHSPTQTIVVNRLGSRRTVVTANPANVEHMLKTNFVNYPKGKPFTDILGDLLGCGI 125

Query: 125 FNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLRLIPLLQNAARTNGVLDLQDVL 184
           FNVDGDLWSTQRKLASHEFSAKS+REFVVK LEDEVHLRLIPLL NAARTN VLDLQDVL
Sbjct: 126 FNVDGDLWSTQRKLASHEFSAKSVREFVVKTLEDEVHLRLIPLLHNAARTNAVLDLQDVL 185

Query: 185 GRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGISAKRAIAPIFLAWKAKRLFNI 244
           GRLAFDT+CKV++G+DEQCLDMSRPVPAI+N+ DTATGISA+RA+AP+F+ WKAKR+ N+
Sbjct: 186 GRLAFDTVCKVTVGTDEQCLDMSRPVPAIVNSLDTATGISARRAVAPLFVTWKAKRMLNV 245

Query: 245 GSEKQLKESVRRVHSWVSDIIRNKN------LDTNKPAGDLLSRLLCAGNDQEVVRDMVV 304
           GSEK+LKE+V  VH WVS+II N+N      LD N   GDLLSRLL AG+ QEVVRDMVV
Sbjct: 246 GSEKKLKEAVEMVHEWVSNIIHNRNLNNSNTLDHNHTHGDLLSRLLSAGHSQEVVRDMVV 305

Query: 305 SFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLS--NKTASFNYGYEELKDMKYL 364
           SFIMAGRDTTSAAMTWLFWLLTKHP+V+  +++E + LS  ++TAS  YGYEELKDMKYL
Sbjct: 306 SFIMAGRDTTSAAMTWLFWLLTKHPKVEQAIVDEAIKLSGDSETASLGYGYEELKDMKYL 365

Query: 365 KACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYFPYGMGRMEDLWGKDRLEF 424
           KACLCESMRLYPPVAWDSKHAAAPD+LPDGT VRKGDRVTYFPYGMGRME+LWGKDRLEF
Sbjct: 366 KACLCESMRLYPPVAWDSKHAAAPDILPDGTPVRKGDRVTYFPYGMGRMEELWGKDRLEF 425

Query: 425 KPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFEFRPVSESPP 484
           KPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYV+ATVLKRFEFRP +E+ P
Sbjct: 426 KPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVMATVLKRFEFRPANENAP 485

BLAST of Sed0010583 vs. ExPASy Swiss-Prot
Match: Q9SMP5 (Cytochrome P450 94B3 OS=Arabidopsis thaliana OX=3702 GN=CYP94B3 PE=1 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 1.7e-170
Identity = 292/466 (62.66%), Postives = 359/466 (77.04%), Query Frame = 0

Query: 24  YPFISCLVSFHKNRRRLLSWYTDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNF 83
           YP I  ++SF+KNR RLL WYT+LL  S ++TI+V  LG+RRTI+T NP NVEY+LKTNF
Sbjct: 39  YPLIGSILSFNKNRHRLLQWYTELLRLSPSQTILVPLLGNRRTIITTNPLNVEYILKTNF 98

Query: 84  VNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLR 143
            N+PKGKPFTD+LGDLLG GIFNVDG  WS+QRKLASHEFS +SLR F  ++L+DEV  R
Sbjct: 99  FNFPKGKPFTDLLGDLLGGGIFNVDGHSWSSQRKLASHEFSTRSLRSFAFEVLKDEVENR 158

Query: 144 LIPLLQNAARTNGVLDLQDVLGRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGI 203
           L+P+L  AA     +DLQDVL R AFD +CKVSLG D  CLD++RPV  ++ AFDTA  I
Sbjct: 159 LVPVLSTAADVGTTVDLQDVLKRFAFDVVCKVSLGWDPDCLDLTRPVNPLVEAFDTAAEI 218

Query: 204 SAKRAIAPIFLAWKAKRLFNIGSEKQLKESVRRVHSWVSDIIRNKNLDTNKPAG-----D 263
           SA+RA  PI+  WK KR+ N+GSE++L+E++R VH  VS+I+R K        G     D
Sbjct: 219 SARRATEPIYAVWKTKRVLNVGSERKLREAIRTVHVLVSEIVRAKKKSLEIGTGAEAKQD 278

Query: 264 LLSRLLCAGNDQEVVRDMVVSFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLSN 323
           LLSR L AG++ E VRDMV+SFIMAGRDTTSAAMTWLFWLLT++ +V+  ++ EV  L  
Sbjct: 279 LLSRFLAAGHNGEAVRDMVISFIMAGRDTTSAAMTWLFWLLTENDDVERKILEEVDPL-- 338

Query: 324 KTASFNYGYEELKDMKYLKACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYF 383
              S   G+E+LK+M Y KACLCE+MRLYPPV+WDSKHAA  DVLPDGTRV++GD+VTYF
Sbjct: 339 --VSLGLGFEDLKEMAYTKACLCEAMRLYPPVSWDSKHAANDDVLPDGTRVKRGDKVTYF 398

Query: 384 PYGMGRMEDLWGKDRLEFKPDRWFQNGE------LKPVSPFKFPVFQAGPRMCLGKEMAF 443
           PYGMGRME LWG D  EF P+RWF +        LKP+SP+KFPVFQAGPR+C+GKEMAF
Sbjct: 399 PYGMGRMETLWGTDSEEFNPNRWFDSEPGSTRPVLKPISPYKFPVFQAGPRVCVGKEMAF 458

Query: 444 IQMKYVVATVLKRFEFRPVSESPPVFVPLLTAHMAGGLQVYVRERT 479
           +QMKYVV +VL RFE  PV++  PVFVPLLTAHMAGGL+V ++ R+
Sbjct: 459 MQMKYVVGSVLSRFEIVPVNKDRPVFVPLLTAHMAGGLKVKIKRRS 500

BLAST of Sed0010583 vs. ExPASy Swiss-Prot
Match: Q9FMV7 (Cytochrome P450 94B1 OS=Arabidopsis thaliana OX=3702 GN=CYP94B1 PE=1 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 2.3e-164
Identity = 287/466 (61.59%), Postives = 354/466 (75.97%), Query Frame = 0

Query: 24  YPFISCLVSFHKNRRRLLSWYTDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNF 83
           Y  I  ++SF+KNR RLL WYTDLL  S ++TI V  L  RRTI+TANP NVE++LKTNF
Sbjct: 41  YQLIGSILSFNKNRHRLLQWYTDLLRLSPSQTITVDLLFGRRTIITANPENVEHILKTNF 100

Query: 84  VNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLR 143
            N+PKGKPFTD+LGDLLG GIFN DG+LWS+QRKLASHEF+ +SLREF  +IL +EV  R
Sbjct: 101 YNFPKGKPFTDLLGDLLGGGIFNSDGELWSSQRKLASHEFTMRSLREFTFEILREEVQNR 160

Query: 144 LIPLLQNAARTNGVLDLQDVLGRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGI 203
           LIP+L +A      +D Q+VL R AFD +CKVSLG D  CLD++RPVP ++ AFD A  I
Sbjct: 161 LIPVLSSAVDCGETVDFQEVLKRFAFDVVCKVSLGWDPDCLDLTRPVPELVKAFDVAAEI 220

Query: 204 SAKRAIAPIFLAWKAKRLFNIGSEKQLKESVRRVHSWVSDIIR--NKNLDTNKPAG---D 263
           SA+RA  P++  WK KR  N+GSEK+L+E+++ VH  VS+IIR   K+LD         D
Sbjct: 221 SARRATEPVYAVWKVKRFLNVGSEKRLREAIKTVHLSVSEIIRAKKKSLDIGGDVSDKQD 280

Query: 264 LLSRLLCAGNDQEVVRDMVVSFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLSN 323
           LLSR L AG+ +E VRD V+SFIMAGRDTTSAAMTWLFWLL+++ +V+  +++E   L N
Sbjct: 281 LLSRFLAAGHGEEAVRDSVISFIMAGRDTTSAAMTWLFWLLSQNDDVETKILDE---LRN 340

Query: 324 KTASFNYGYEELKDMKYLKACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYF 383
           K  S   G+E+L++M Y KACLCE+MRLYPPVAWDSKHAA  D+LPDGT ++KGD+VTYF
Sbjct: 341 K-GSLGLGFEDLREMSYTKACLCEAMRLYPPVAWDSKHAANDDILPDGTPLKKGDKVTYF 400

Query: 384 PYGMGRMEDLWGKDRLEFKPDRWFQNGE-------LKPVSPFKFPVFQAGPRMCLGKEMA 443
           PYGMGRME +WGKD  EFKP+RWF+          LK VS FKFPVFQAGPR+C+GKEMA
Sbjct: 401 PYGMGRMEKVWGKDWDEFKPNRWFEEEPSYGTKPVLKSVSSFKFPVFQAGPRVCIGKEMA 460

Query: 444 FIQMKYVVATVLKRFEFRPVSESPPVFVPLLTAHMAGGLQVYVRER 478
           F QMKYVV +VL RF+  PV  + PVFVPLLTAHMAGGL+V ++ R
Sbjct: 461 FTQMKYVVGSVLSRFKIIPVCNNRPVFVPLLTAHMAGGLKVKIKRR 502

BLAST of Sed0010583 vs. ExPASy Swiss-Prot
Match: Q9ZUX1 (Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 4.0e-116
Identity = 234/489 (47.85%), Postives = 310/489 (63.39%), Query Frame = 0

Query: 7   ISLFFLLCLSLCAFFFLYPFISC---------LVSFHKNRRRLLSWYTDLLSDSSTRTIV 66
           +S FF++  SL    FL     C           S+ K+   L  WYT LL  S T TI 
Sbjct: 10  VSFFFIIIFSLFHLLFLQKLRYCNCEICHAYLTSSWKKDFINLSDWYTHLLRRSPTSTIK 69

Query: 67  VHRLGSRRTIVTANPANVEYMLKTNFVNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 126
           VH L S   ++TANP+NVE++LKTNF NYPKGK F+ ILGDLLG GIFN DGD W  QRK
Sbjct: 70  VHVLNS---VITANPSNVEHILKTNFHNYPKGKQFSVILGDLLGRGIFNSDGDTWRFQRK 129

Query: 127 LASHEFSAKSLREFVVKILEDEVHLRLIPLLQNAARTNG-VLDLQDVLGRLAFDTICKVS 186
           LAS E  + S+R F  +I++ E+  RL+P+L + +   G VLDLQDV  R +FDTI K+S
Sbjct: 130 LASLELGSVSVRVFAHEIVKTEIETRLLPILTSFSDNPGSVLDLQDVFRRFSFDTISKLS 189

Query: 187 LGSDEQCLDMSRPVPAILNAFDTATGISAKRAIAPIFLAWKAKRLFNIGSEKQLKESVRR 246
            G D  CL +  P+     AFDTA+ +SAKRA+AP  L WK KRL  IGSEK+L+ES+  
Sbjct: 190 FGFDPDCLRLPFPISEFAVAFDTASLLSAKRALAPFPLLWKTKRLLRIGSEKKLQESINV 249

Query: 247 VHSWVSDIIRNKNLDTNKPAGDLLSRLLC--AGNDQEVVRDMVVSFIMAGRDTTSAAMTW 306
           ++    D+I+ + L       DL+SR +   A +D E +RD+VVSF++AGRDT +A +T 
Sbjct: 250 INRLAGDLIKQRRLTGLMGKNDLISRFMAVVAEDDDEYLRDIVVSFLLAGRDTVAAGLTG 309

Query: 307 LFWLLTKHPEVQATLMNEVVS-LSNKTASFNYGYEELKDMKYLKACLCESMRLYPPVAWD 366
            FWLLT+HPEV+  +  E+   +     S     +E+++M YL A L ESMRL+PPV +D
Sbjct: 310 FFWLLTRHPEVENRIREELDRVMGTGFDSVTARCDEMREMDYLHASLYESMRLFPPVQFD 369

Query: 367 SKHAAAPDVLPDGTRVRKGDRVTYFPYGMGRMEDLWGKDRLEFKPDRWFQN-GELKPVSP 426
           SK A   DVL DGT V  G RVTY  Y MGRM+ +WG D  EFKP+RW  N G+ +P +P
Sbjct: 370 SKFALNDDVLSDGTFVNSGTRVTYHAYAMGRMDRIWGPDYEEFKPERWLDNEGKFRPENP 429

Query: 427 FKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFEFR---PVSESPPVFVPLLTAHMAGG 479
            K+PVFQAG R+C+GKEMA ++MK +   +++RFE R   P +     F P LTA + GG
Sbjct: 430 VKYPVFQAGARVCIGKEMAIMEMKSIAVAIIRRFETRVASPETTETLRFAPGLTATVNGG 489

BLAST of Sed0010583 vs. ExPASy Swiss-Prot
Match: O81117 (Cytochrome P450 94A1 OS=Vicia sativa OX=3908 GN=CYP94A1 PE=2 SV=2)

HSP 1 Score: 408.3 bits (1048), Expect = 1.2e-112
Identity = 209/467 (44.75%), Postives = 294/467 (62.96%), Query Frame = 0

Query: 24  YPFISCLVSFHKNRRRLLSWYTDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNF 83
           YP I   +SF KN  R + W +D++  S + T  +     +R I+T NP+ V+++LK  F
Sbjct: 51  YPLIGSYLSFRKNLHRRIQWLSDIVQISPSATFQLDGTLGKRQIITGNPSTVQHILKNQF 110

Query: 84  VNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLR 143
            NY KG  FT+ L D LG GIFN +G  W  QR++ASHEF+ KS+R FV  I++ E+  R
Sbjct: 111 SNYQKGTTFTNTLSDFLGTGIFNTNGPNWKFQRQVASHEFNTKSIRNFVEHIVDTELTNR 170

Query: 144 LIPLLQNAARTNGVLDLQDVLGRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGI 203
           LIP+L ++ +TN +LD QD+L R  FD IC ++ G D + L  S        A++ AT I
Sbjct: 171 LIPILTSSTQTNNILDFQDILQRFTFDNICNIAFGYDPEYLTPSTNRSKFAEAYEDATEI 230

Query: 204 SAKRAIAPIFLAWKAKRLFNIGSEKQLKESVRRVHSWVSDIIRNKNLDTNKPAG----DL 263
           S+KR   P+ + WK K+ FNIGSEK+LKE+V  V S+   ++R K  +  + +     D+
Sbjct: 231 SSKRFRLPLPIIWKIKKYFNIGSEKRLKEAVTEVRSFAKKLVREKKRELEEKSSLETEDM 290

Query: 264 LSRLLCAG-NDQEVVRDMVVSFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLSN 323
           LSR L +G +D++ V D+V+SFI+AG+DTTSAA+TW FWLL K+P V+  ++NE+   S 
Sbjct: 291 LSRFLSSGHSDEDFVADIVISFILAGKDTTSAALTWFFWLLWKNPRVEEEIVNELSKKSE 350

Query: 324 KTASFNYGYEELKDMKYLKACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYF 383
                   Y+E+K+M Y  A L ESMRLYPPV  DSK A   DVLPDG  V+KG  VTY 
Sbjct: 351 LMV-----YDEVKEMVYTHAALSESMRLYPPVPMDSKEAVNDDVLPDGWVVKKGTIVTYH 410

Query: 384 PYGMGRMEDLWGKDRLEFKPDRWFQNGELKPV------SPFKFPVFQAGPRMCLGKEMAF 443
            Y MGRM+ LWG D  EF+P+RW +  E+           + +PVFQAGPR+CLGKEMAF
Sbjct: 411 VYAMGRMKSLWGDDWAEFRPERWLEKDEVNGKWVFVGRDSYSYPVFQAGPRVCLGKEMAF 470

Query: 444 IQMKYVVATVLKRFEFRPVSE--SPPVFVPLLTAHMAGGLQVYVRER 478
           +QMK +VA ++ +F+  P +     P F+  L++ M GG  V +++R
Sbjct: 471 MQMKRIVAGIVGKFKVVPEAHLAQEPGFISFLSSQMEGGFPVTIQKR 512

BLAST of Sed0010583 vs. ExPASy Swiss-Prot
Match: P98188 (Cytochrome P450 94A2 OS=Vicia sativa OX=3908 GN=CYP94A2 PE=2 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 6.3e-109
Identity = 210/470 (44.68%), Postives = 284/470 (60.43%), Query Frame = 0

Query: 24  YPFISCLVSFHKNRRRLLSWYTDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNF 83
           YP      S   N  R + W +D+L    + T V+HR    R + TA PA V+++L+TNF
Sbjct: 46  YPIFGSAFSLLANFHRRIQWTSDILQTIPSSTFVLHRPFGARQVFTAQPAVVQHILRTNF 105

Query: 84  VNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLR 143
             Y KG  F   + D LG GIFN DG+ W  QR+++SHEF+ +SLR+FV  +++ E+  R
Sbjct: 106 TCYGKGLTFYQSINDFLGDGIFNADGESWKFQRQISSHEFNTRSLRKFVETVVDVELSDR 165

Query: 144 LIPLLQNAARTNGVLDLQDVLGRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGI 203
           L+P+L  A+ +   LD QD+L RL FD IC ++ G D + L  S P      AFD ++ +
Sbjct: 166 LVPVLSQASNSQTTLDFQDILQRLTFDNICMIAFGYDPEYLLPSLPEIPFAKAFDESSQL 225

Query: 204 SAKRAIAPIFLAWKAKRLFNIGSEKQLKESVRRVHSWVSDIIRNKN-------LDTNKPA 263
           S +R  A I L WK KR  NIG E+QLKE+V  V    + I++NK        L +   +
Sbjct: 226 SIERLNALIPLLWKVKRFLNIGVERQLKEAVAEVRGLATKIVKNKKKELKEKALQSESES 285

Query: 264 GDLLSRLLCAG-NDQEVVRDMVVSFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVS 323
            DLLSR L +G +D+  V DMV+S I+AGRDTTSAA+TW FWLL+KH  V+  ++ E+  
Sbjct: 286 VDLLSRFLSSGHSDESFVTDMVISIILAGRDTTSAALTWFFWLLSKHSHVENEILKEITG 345

Query: 324 LSNKTASFNYGYEELKDMKYLKACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRV 383
            S        GY+E+KDM Y  A LCESMRLYPP+  D+K A   DVLPDGT V+KG RV
Sbjct: 346 KSE-----TVGYDEVKDMVYTHAALCESMRLYPPLPVDTKVAVHDDVLPDGTLVKKGWRV 405

Query: 384 TYFPYGMGRMEDLWGKDRLEFKPDRWFQNGELKP-----VSPFKFPVFQAGPRMCLGKEM 443
           TY  Y MGR E +WG D  EF+P+RW    E+       +  + +PVFQAGPR+C+GKEM
Sbjct: 406 TYHIYAMGRSEKIWGPDWAEFRPERWLSRDEVGKWSFVGIDYYSYPVFQAGPRVCIGKEM 465

Query: 444 AFIQMKYVVATVLKRFEFRP--VSESPPVFVPLLTAHMAGGLQVYVRERT 479
           AF+QMK VVA ++ RF   P  V    P +    T+ M GG  V + +R+
Sbjct: 466 AFLQMKRVVAGIMGRFRVVPAMVEGIEPEYTAHFTSVMKGGFPVKIEKRS 510

BLAST of Sed0010583 vs. ExPASy TrEMBL
Match: A0A6J1HMR4 (cytochrome P450 94B1-like OS=Cucurbita maxima OX=3661 GN=LOC111466028 PE=4 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 5.5e-233
Identity = 409/499 (81.96%), Postives = 441/499 (88.38%), Query Frame = 0

Query: 4   NFGISLFFLLCLSLCAFFFL-------------------YPFISCLVSFHKNRRRLLSWY 63
           +  +SLF LL +SL AFF +                   YPFI C +SF KNRRRLLSWY
Sbjct: 3   DLAVSLFLLLSISLWAFFLIYVFIYKFHRFSTNRSSPPSYPFIGCFISFFKNRRRLLSWY 62

Query: 64  TDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNFVNYPKGKPFTDILGDLLGCGI 123
           T LLS+S + TIVVHRLGSRRT+VTANPANVEYMLKTNF+NYPKGKPFTDILGDLLGCGI
Sbjct: 63  THLLSNSPSLTIVVHRLGSRRTVVTANPANVEYMLKTNFLNYPKGKPFTDILGDLLGCGI 122

Query: 124 FNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLRLIPLLQNAARTNGVLDLQDVL 183
           FNVDG+LWSTQRKLASHEFSAKSLREFVVK LEDE+HLRLIPLLQNAARTNGVLDLQDVL
Sbjct: 123 FNVDGELWSTQRKLASHEFSAKSLREFVVKTLEDEIHLRLIPLLQNAARTNGVLDLQDVL 182

Query: 184 GRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGISAKRAIAPIFLAWKAKRLFNI 243
           GRLAFDT+CK++LG+DEQCLDMSRPVPAI+NAFD ATGISAKRAIAP+ LAWKAKRLFNI
Sbjct: 183 GRLAFDTVCKMTLGTDEQCLDMSRPVPAIVNAFDMATGISAKRAIAPVSLAWKAKRLFNI 242

Query: 244 GSEKQLKESVRRVHSWVSDIIRNKNLDTNKPAGDLLSRLLCAGNDQEVVRDMVVSFIMAG 303
           GSEK+LKE+V+ VHSWVS IIRNKNLD N+ + DLLSRLL AG+ +EVVRDMVVSFIMAG
Sbjct: 243 GSEKRLKEAVQMVHSWVSHIIRNKNLDNNEDS-DLLSRLLSAGHGEEVVRDMVVSFIMAG 302

Query: 304 RDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLSN--KTASFNYGYEELKDMKYLKACLCE 363
           RDTTSAAMTWLFWLLTKHP+V+ TLM+EV SLSN  +TAS  YGYEELKDMKYLKACLCE
Sbjct: 303 RDTTSAAMTWLFWLLTKHPKVEQTLMDEVASLSNNYQTASLGYGYEELKDMKYLKACLCE 362

Query: 364 SMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYFPYGMGRMEDLWGKDRLEFKPDRWF 423
           SMRLYPPVAWDSKHAAA D+LPDGT VRKGDRVTYFPYGMGRME+LWG DR EFKP+RWF
Sbjct: 363 SMRLYPPVAWDSKHAAAADILPDGTPVRKGDRVTYFPYGMGRMEELWGPDREEFKPERWF 422

Query: 424 QNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFEFRPVSESPPVFVPLL 482
           + GE K VSPFKFPVFQ GPRMCLGKEMAFIQMKYVVATVLKRFEFR VSES PVFVPLL
Sbjct: 423 EKGEAKAVSPFKFPVFQGGPRMCLGKEMAFIQMKYVVATVLKRFEFRAVSESEPVFVPLL 482

BLAST of Sed0010583 vs. ExPASy TrEMBL
Match: A0A6J1E0I0 (cytochrome P450 94B3 OS=Cucurbita moschata OX=3662 GN=LOC111429724 PE=3 SV=1)

HSP 1 Score: 813.1 bits (2099), Expect = 6.1e-232
Identity = 402/508 (79.13%), Postives = 444/508 (87.40%), Query Frame = 0

Query: 5   FGISLFFLLCLSLCAFFFL--------------------YPFISCLVSFHKNRRRLLSWY 64
           F +SLFFLL LS  AFF L                    YPFI CL+SF+KNRRRLLSWY
Sbjct: 6   FALSLFFLLSLSSTAFFCLYLSFFRFYCFSKNRFYSPPSYPFIGCLISFYKNRRRLLSWY 65

Query: 65  TDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNFVNYPKGKPFTDILGDLLGCGI 124
           T LLS S T+TIVV+RLGSRRT+VTANPANVE+MLKTNFVNYPKGKPFTDILGDLLGCGI
Sbjct: 66  THLLSHSPTQTIVVNRLGSRRTVVTANPANVEHMLKTNFVNYPKGKPFTDILGDLLGCGI 125

Query: 125 FNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLRLIPLLQNAARTNGVLDLQDVL 184
           FNVDGDLWSTQRKLASHEFSAKS+REFVVK LEDEVHLRLIPLL NAARTN VLDLQDVL
Sbjct: 126 FNVDGDLWSTQRKLASHEFSAKSVREFVVKTLEDEVHLRLIPLLHNAARTNAVLDLQDVL 185

Query: 185 GRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGISAKRAIAPIFLAWKAKRLFNI 244
           GRLAFDT+CKV++G+DEQCLDMSRP+PAI+N+ DTATGISA+RA+AP+F+ WKAKR+ N+
Sbjct: 186 GRLAFDTVCKVTVGTDEQCLDMSRPIPAIVNSLDTATGISARRAVAPLFVTWKAKRMLNV 245

Query: 245 GSEKQLKESVRRVHSWVSDIIRNKN------LDTNKPAGDLLSRLLCAGNDQEVVRDMVV 304
           GSEK+LKE+V  VH WVS+IIRN+N      LD N   GDLLSRLL AG+ QEV+RDMVV
Sbjct: 246 GSEKKLKEAVEMVHEWVSNIIRNRNLNNSNTLDHNHAHGDLLSRLLSAGHSQEVIRDMVV 305

Query: 305 SFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLS--NKTASFNYGYEELKDMKYL 364
           SFIMAGRDTTSAAMTWLFWLLTKHP+V+  +++E + LS  ++TAS  YGYEELKDMKYL
Sbjct: 306 SFIMAGRDTTSAAMTWLFWLLTKHPKVEQAIVDEAIKLSGDSETASLGYGYEELKDMKYL 365

Query: 365 KACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYFPYGMGRMEDLWGKDRLEF 424
           KACLCESMRLYPPVAWDSKHAAAPD+LPDGT VRKGDRVTYFPYGMGRME+LWGKDRLEF
Sbjct: 366 KACLCESMRLYPPVAWDSKHAAAPDILPDGTPVRKGDRVTYFPYGMGRMEELWGKDRLEF 425

Query: 425 KPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFEFRPVSESPP 484
           KPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYV+ATVLKRFEFRP +E+ P
Sbjct: 426 KPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVMATVLKRFEFRPANENAP 485

BLAST of Sed0010583 vs. ExPASy TrEMBL
Match: A0A6J1JAK9 (cytochrome P450 94B3-like OS=Cucurbita maxima OX=3661 GN=LOC111485059 PE=3 SV=1)

HSP 1 Score: 812.0 bits (2096), Expect = 1.3e-231
Identity = 404/508 (79.53%), Postives = 443/508 (87.20%), Query Frame = 0

Query: 5   FGISLFFLLCLSLCAFFFL--------------------YPFISCLVSFHKNRRRLLSWY 64
           F + LFFLL LS  AFF L                    YPFI CL+SF+KNRRRLLSWY
Sbjct: 6   FALFLFFLLSLSSTAFFCLYLSFFKFYCFSKNLFYSPPSYPFIGCLISFYKNRRRLLSWY 65

Query: 65  TDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNFVNYPKGKPFTDILGDLLGCGI 124
           T LLS S T+TIVV+RLGSRRT+VTANPANVE+MLKTNFVNYPKGKPFTDILGDLLGCGI
Sbjct: 66  THLLSHSPTQTIVVNRLGSRRTVVTANPANVEHMLKTNFVNYPKGKPFTDILGDLLGCGI 125

Query: 125 FNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLRLIPLLQNAARTNGVLDLQDVL 184
           FNVDGDLWSTQRKLASHEFSAKS+REFVVK LEDEVHLRLIPLLQNAARTN VLDLQDVL
Sbjct: 126 FNVDGDLWSTQRKLASHEFSAKSVREFVVKTLEDEVHLRLIPLLQNAARTNAVLDLQDVL 185

Query: 185 GRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGISAKRAIAPIFLAWKAKRLFNI 244
           GRLAFDT+CKV++G+DEQCLDMSRP+PAI+N+ DTATGISA RA+AP+FL WKAKR+ N+
Sbjct: 186 GRLAFDTVCKVTVGTDEQCLDMSRPLPAIVNSLDTATGISAGRAVAPLFLTWKAKRMLNV 245

Query: 245 GSEKQLKESVRRVHSWVSDIIRNKN------LDTNKPAGDLLSRLLCAGNDQEVVRDMVV 304
           GSEK+LKE+V  VH WVS+IIRN+N      LD N   GDLLSRLL AG+ QEVVRDMVV
Sbjct: 246 GSEKKLKEAVEMVHEWVSNIIRNRNLNNSNTLDHNHTHGDLLSRLLSAGHSQEVVRDMVV 305

Query: 305 SFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLS--NKTASFNYGYEELKDMKYL 364
           SFIMAGRDTTSAAMTWLFWLLTKHP+V+  +++E + LS  ++TAS  YGYEELKDMKYL
Sbjct: 306 SFIMAGRDTTSAAMTWLFWLLTKHPKVEQVIVDEAIKLSGDSETASLGYGYEELKDMKYL 365

Query: 365 KACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYFPYGMGRMEDLWGKDRLEF 424
           KACLCESMRLYPPVAWDSKHAAAPD+LPDGT VRKGDRVTYFPYGMGRME+LWGKDRLEF
Sbjct: 366 KACLCESMRLYPPVAWDSKHAAAPDILPDGTPVRKGDRVTYFPYGMGRMEELWGKDRLEF 425

Query: 425 KPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFEFRPVSESPP 484
           KPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYV+ATVLKRFEFRP +E+ P
Sbjct: 426 KPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVMATVLKRFEFRPANENAP 485

BLAST of Sed0010583 vs. ExPASy TrEMBL
Match: A0A6J1F533 (cytochrome P450 94B3-like OS=Cucurbita moschata OX=3662 GN=LOC111440917 PE=4 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.8e-229
Identity = 407/509 (79.96%), Postives = 437/509 (85.85%), Query Frame = 0

Query: 4   NFGISLFFLLCLSLCAFFFL-----------------------------YPFISCLVSFH 63
           +  +SLF LL LSL AF  +                             YPFI C +SF 
Sbjct: 3   DLAVSLFLLLSLSLWAFCLIYVFIYIFHRSSTDRSSTDRSSTDRSSPPSYPFIGCFISFF 62

Query: 64  KNRRRLLSWYTDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNFVNYPKGKPFTD 123
           KNRRRLLSWYT LLS S + TIVVHRLGSRRT+VTANPANVEYMLKTNF+NYPKGKPFTD
Sbjct: 63  KNRRRLLSWYTHLLSHSPSLTIVVHRLGSRRTVVTANPANVEYMLKTNFLNYPKGKPFTD 122

Query: 124 ILGDLLGCGIFNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLRLIPLLQNAART 183
           ILGDLLGCGIFNVDG+LWSTQRKLASHEFSAKSLREFVVK LEDEVHLRLIPLLQ AART
Sbjct: 123 ILGDLLGCGIFNVDGELWSTQRKLASHEFSAKSLREFVVKTLEDEVHLRLIPLLQKAART 182

Query: 184 NGVLDLQDVLGRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGISAKRAIAPIFL 243
           N  LDLQDVLGRLAFDTICK++LG+DEQCLD+SRPVPAI+NAFD ATGISAKRAIAP+ L
Sbjct: 183 NAALDLQDVLGRLAFDTICKMTLGTDEQCLDLSRPVPAIVNAFDMATGISAKRAIAPVSL 242

Query: 244 AWKAKRLFNIGSEKQLKESVRRVHSWVSDIIRNKNLDTNKPAGDLLSRLLCAGNDQEVVR 303
           AWKAKRLFNIGSEK+LKE+V+ VHSWVS IIRNKNLD NK + DLLSRLL AG+ +EVVR
Sbjct: 243 AWKAKRLFNIGSEKRLKEAVQMVHSWVSHIIRNKNLDNNKDS-DLLSRLLSAGHGEEVVR 302

Query: 304 DMVVSFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLSN--KTASFNYGYEELKD 363
           DMVVSFIMAGRDTTSAAMTWLFWLLTKHP+V+ TLM+EV SLSN  +TAS  YGYEELKD
Sbjct: 303 DMVVSFIMAGRDTTSAAMTWLFWLLTKHPKVEQTLMDEVASLSNNYRTASLGYGYEELKD 362

Query: 364 MKYLKACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYFPYGMGRMEDLWGKD 423
           MKYLKACLCESMRLYPPVAWDSKHAAA D+LPDGT VRKGDRVTYFPYGMGRME+LWG +
Sbjct: 363 MKYLKACLCESMRLYPPVAWDSKHAAAADILPDGTPVRKGDRVTYFPYGMGRMEELWGPE 422

Query: 424 RLEFKPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFEFRPVS 482
           R EFKP+RWF+ GE+K VSPFKFPVFQ GPRMCLGKEMAFIQMKYVVATVLKRFEFR VS
Sbjct: 423 REEFKPERWFEKGEVKAVSPFKFPVFQGGPRMCLGKEMAFIQMKYVVATVLKRFEFRAVS 482

BLAST of Sed0010583 vs. ExPASy TrEMBL
Match: A0A6J1C252 (cytochrome P450 94B3-like OS=Momordica charantia OX=3673 GN=LOC111007253 PE=4 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 8.5e-226
Identity = 398/511 (77.89%), Postives = 434/511 (84.93%), Query Frame = 0

Query: 3   LNFGISLFFLLCLSLCAFFFL-------------------YPFISCLVSFHKNRRRLLSW 62
           L F +SLFFLL LSL  FFFL                   YPFI CL+SF KNRRRLL+W
Sbjct: 5   LGFAMSLFFLLSLSLWTFFFLCICIYKSYCSSVNRFSPPSYPFIGCLISFFKNRRRLLAW 64

Query: 63  YTDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNFVNYPKGKPFTDILGDLLGCG 122
           YTDLLS S TRTIVVHRLGSRRT+VTANPANVEYMLKTNF+N+PKG+PFTDILGDLLGCG
Sbjct: 65  YTDLLSSSPTRTIVVHRLGSRRTVVTANPANVEYMLKTNFLNFPKGQPFTDILGDLLGCG 124

Query: 123 IFNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLRLIPLLQNAARTNGVLDLQDV 182
           IFNVDG+LWSTQRKLASHEFSAKSLREFVVK LE+EVH RLIPLL+NAARTN VLDLQDV
Sbjct: 125 IFNVDGELWSTQRKLASHEFSAKSLREFVVKALEEEVHFRLIPLLENAARTNAVLDLQDV 184

Query: 183 LGRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGISAKRAIAPIFLAWKAKRLFN 242
           LGRLAFDT+CKV+LGSDEQCLDMSR VP  + AFDTAT +SAKRA+APIFLAWKAKR+ N
Sbjct: 185 LGRLAFDTVCKVTLGSDEQCLDMSRAVPGHVKAFDTATEVSAKRAVAPIFLAWKAKRILN 244

Query: 243 IGSEKQLKESVRRVHSWVSDIIRNKNLDTNK---------PAGDLLSRLLCAGNDQEVVR 302
           +GSEK+LKE+V  VH+WVSDIIRNK L +N           +GDLLSRLL AG   E VR
Sbjct: 245 VGSEKKLKEAVHTVHTWVSDIIRNKKLTSNNNNNNTLENDKSGDLLSRLLSAGLGDEEVR 304

Query: 303 DMVVSFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLSNKTASFNYGYEELKDMK 362
           DMVVSFIMAGRDTTSAAMTWLFWLLTKH  V+ T++ EV SLS KT S  Y YE+LK+MK
Sbjct: 305 DMVVSFIMAGRDTTSAAMTWLFWLLTKHRHVEQTIVKEVSSLS-KTTSLGYDYEDLKEMK 364

Query: 363 YLKACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYFPYGMGRMEDLWGKDRL 422
           +LKACLCESMRLYPPVAWDSKHAAA D+LPDGT VRKGDRVTYFPYGMGRME+LWGKDRL
Sbjct: 365 FLKACLCESMRLYPPVAWDSKHAAAQDILPDGTPVRKGDRVTYFPYGMGRMEELWGKDRL 424

Query: 423 EFKPDRWFQNGELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFEFRPVSES 482
           EFKP+RWF+ GELK V+PFKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFE  PVS +
Sbjct: 425 EFKPERWFEKGELKAVNPFKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFELVPVSGN 484

Query: 483 PPVFVPLLTAHMAGGLQVYVRERTGQKSTVW 486
            PVFVPLLTAHMAGGLQVYV ERT QKS++W
Sbjct: 485 VPVFVPLLTAHMAGGLQVYVTERTAQKSSIW 514

BLAST of Sed0010583 vs. TAIR 10
Match: AT3G48520.1 (cytochrome P450, family 94, subfamily B, polypeptide 3 )

HSP 1 Score: 600.5 bits (1547), Expect = 1.2e-171
Identity = 292/466 (62.66%), Postives = 359/466 (77.04%), Query Frame = 0

Query: 24  YPFISCLVSFHKNRRRLLSWYTDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNF 83
           YP I  ++SF+KNR RLL WYT+LL  S ++TI+V  LG+RRTI+T NP NVEY+LKTNF
Sbjct: 39  YPLIGSILSFNKNRHRLLQWYTELLRLSPSQTILVPLLGNRRTIITTNPLNVEYILKTNF 98

Query: 84  VNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLR 143
            N+PKGKPFTD+LGDLLG GIFNVDG  WS+QRKLASHEFS +SLR F  ++L+DEV  R
Sbjct: 99  FNFPKGKPFTDLLGDLLGGGIFNVDGHSWSSQRKLASHEFSTRSLRSFAFEVLKDEVENR 158

Query: 144 LIPLLQNAARTNGVLDLQDVLGRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGI 203
           L+P+L  AA     +DLQDVL R AFD +CKVSLG D  CLD++RPV  ++ AFDTA  I
Sbjct: 159 LVPVLSTAADVGTTVDLQDVLKRFAFDVVCKVSLGWDPDCLDLTRPVNPLVEAFDTAAEI 218

Query: 204 SAKRAIAPIFLAWKAKRLFNIGSEKQLKESVRRVHSWVSDIIRNKNLDTNKPAG-----D 263
           SA+RA  PI+  WK KR+ N+GSE++L+E++R VH  VS+I+R K        G     D
Sbjct: 219 SARRATEPIYAVWKTKRVLNVGSERKLREAIRTVHVLVSEIVRAKKKSLEIGTGAEAKQD 278

Query: 264 LLSRLLCAGNDQEVVRDMVVSFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLSN 323
           LLSR L AG++ E VRDMV+SFIMAGRDTTSAAMTWLFWLLT++ +V+  ++ EV  L  
Sbjct: 279 LLSRFLAAGHNGEAVRDMVISFIMAGRDTTSAAMTWLFWLLTENDDVERKILEEVDPL-- 338

Query: 324 KTASFNYGYEELKDMKYLKACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYF 383
              S   G+E+LK+M Y KACLCE+MRLYPPV+WDSKHAA  DVLPDGTRV++GD+VTYF
Sbjct: 339 --VSLGLGFEDLKEMAYTKACLCEAMRLYPPVSWDSKHAANDDVLPDGTRVKRGDKVTYF 398

Query: 384 PYGMGRMEDLWGKDRLEFKPDRWFQNGE------LKPVSPFKFPVFQAGPRMCLGKEMAF 443
           PYGMGRME LWG D  EF P+RWF +        LKP+SP+KFPVFQAGPR+C+GKEMAF
Sbjct: 399 PYGMGRMETLWGTDSEEFNPNRWFDSEPGSTRPVLKPISPYKFPVFQAGPRVCVGKEMAF 458

Query: 444 IQMKYVVATVLKRFEFRPVSESPPVFVPLLTAHMAGGLQVYVRERT 479
           +QMKYVV +VL RFE  PV++  PVFVPLLTAHMAGGL+V ++ R+
Sbjct: 459 MQMKYVVGSVLSRFEIVPVNKDRPVFVPLLTAHMAGGLKVKIKRRS 500

BLAST of Sed0010583 vs. TAIR 10
Match: AT5G63450.1 (cytochrome P450, family 94, subfamily B, polypeptide 1 )

HSP 1 Score: 580.1 bits (1494), Expect = 1.7e-165
Identity = 287/466 (61.59%), Postives = 354/466 (75.97%), Query Frame = 0

Query: 24  YPFISCLVSFHKNRRRLLSWYTDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNF 83
           Y  I  ++SF+KNR RLL WYTDLL  S ++TI V  L  RRTI+TANP NVE++LKTNF
Sbjct: 41  YQLIGSILSFNKNRHRLLQWYTDLLRLSPSQTITVDLLFGRRTIITANPENVEHILKTNF 100

Query: 84  VNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLR 143
            N+PKGKPFTD+LGDLLG GIFN DG+LWS+QRKLASHEF+ +SLREF  +IL +EV  R
Sbjct: 101 YNFPKGKPFTDLLGDLLGGGIFNSDGELWSSQRKLASHEFTMRSLREFTFEILREEVQNR 160

Query: 144 LIPLLQNAARTNGVLDLQDVLGRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGI 203
           LIP+L +A      +D Q+VL R AFD +CKVSLG D  CLD++RPVP ++ AFD A  I
Sbjct: 161 LIPVLSSAVDCGETVDFQEVLKRFAFDVVCKVSLGWDPDCLDLTRPVPELVKAFDVAAEI 220

Query: 204 SAKRAIAPIFLAWKAKRLFNIGSEKQLKESVRRVHSWVSDIIR--NKNLDTNKPAG---D 263
           SA+RA  P++  WK KR  N+GSEK+L+E+++ VH  VS+IIR   K+LD         D
Sbjct: 221 SARRATEPVYAVWKVKRFLNVGSEKRLREAIKTVHLSVSEIIRAKKKSLDIGGDVSDKQD 280

Query: 264 LLSRLLCAGNDQEVVRDMVVSFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLSN 323
           LLSR L AG+ +E VRD V+SFIMAGRDTTSAAMTWLFWLL+++ +V+  +++E   L N
Sbjct: 281 LLSRFLAAGHGEEAVRDSVISFIMAGRDTTSAAMTWLFWLLSQNDDVETKILDE---LRN 340

Query: 324 KTASFNYGYEELKDMKYLKACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYF 383
           K  S   G+E+L++M Y KACLCE+MRLYPPVAWDSKHAA  D+LPDGT ++KGD+VTYF
Sbjct: 341 K-GSLGLGFEDLREMSYTKACLCEAMRLYPPVAWDSKHAANDDILPDGTPLKKGDKVTYF 400

Query: 384 PYGMGRMEDLWGKDRLEFKPDRWFQNGE-------LKPVSPFKFPVFQAGPRMCLGKEMA 443
           PYGMGRME +WGKD  EFKP+RWF+          LK VS FKFPVFQAGPR+C+GKEMA
Sbjct: 401 PYGMGRMEKVWGKDWDEFKPNRWFEEEPSYGTKPVLKSVSSFKFPVFQAGPRVCIGKEMA 460

Query: 444 FIQMKYVVATVLKRFEFRPVSESPPVFVPLLTAHMAGGLQVYVRER 478
           F QMKYVV +VL RF+  PV  + PVFVPLLTAHMAGGL+V ++ R
Sbjct: 461 FTQMKYVVGSVLSRFKIIPVCNNRPVFVPLLTAHMAGGLKVKIKRR 502

BLAST of Sed0010583 vs. TAIR 10
Match: AT3G01900.1 (cytochrome P450, family 94, subfamily B, polypeptide 2 )

HSP 1 Score: 537.3 bits (1383), Expect = 1.2e-152
Identity = 268/461 (58.13%), Postives = 334/461 (72.45%), Query Frame = 0

Query: 24  YPFISCLVSFHKNRRRLLSWYTDLLSDSSTRTIVVHRLGSRRTIVTANPANVEYMLKTNF 83
           YP I CL+SF+ NR RLL WYT+LL++S +RT+V+ RL +RRT+VTANP+NVEY+LKTNF
Sbjct: 35  YPVIGCLISFYTNRNRLLDWYTELLTESPSRTVVIRRLAARRTVVTANPSNVEYILKTNF 94

Query: 84  VNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLASHEFSAKSLREFVVKILEDEVHLR 143
            NYPKGKPFT+ILGD LG GIFNVDG+LW  QR+LA+H+F+ KSLRE+V  +L +EV   
Sbjct: 95  DNYPKGKPFTEILGDFLGNGIFNVDGNLWLKQRRLATHDFTPKSLREYVT-VLRNEVEKE 154

Query: 144 LIPLLQNAARTNGVLDLQDVLGRLAFDTICKVSLGSDEQCLDMSRPVPAILNAFDTATGI 203
           L+  L  AA  +   DLQ++L R  F+ +C V LG D   L+ S PV     AF TA+ +
Sbjct: 155 LLAFLNAAAEDSQPFDLQELLRRFTFNIVCIVFLGIDRCTLNPSSPVSEFDRAFQTASAV 214

Query: 204 SAKRAIAPIFLAWKAKRLFNIGSEKQLKESVRRVHSWVSDIIRNKNLDTNKPAG-DLLSR 263
           SA R  AP+   WK KRL   GSEK+L+++V  VH+ V +IIR+K     KPA  D LSR
Sbjct: 215 SAGRGSAPLSFVWKFKRLVGFGSEKELRKAVGEVHNCVDEIIRDKK---RKPANQDFLSR 274

Query: 264 LLCAGNDQEVVRDMVVSFIMAGRDTTSAAMTWLFWLLTKHPEVQATLMNEVVSLSNKTAS 323
           L+ AG   E VRDMV+S IMAGRDTTSA  T LFWL+T H E +  L++E+ S+  +   
Sbjct: 275 LIVAGESDETVRDMVISIIMAGRDTTSAVATRLFWLITGHEETEHDLVSEIRSVKEEITG 334

Query: 324 FNYGYEELKDMKYLKACLCESMRLYPPVAWDSKHAAAPDVLPDGTRVRKGDRVTYFPYGM 383
             + YE LK +  LKACLCE MRLYPPV WDSKHA   D LPDGT VR GDRVTYFPYGM
Sbjct: 335 -GFDYESLKKLSLLKACLCEVMRLYPPVPWDSKHALTDDRLPDGTLVRAGDRVTYFPYGM 394

Query: 384 GRMEDLWGKDRLEFKPDRWFQNGE------LKPVSPFKFPVFQAGPRMCLGKEMAFIQMK 443
           GRME+LWG+D  EFKP+RW ++ +      LK V+PFKFPVFQAGPR+CLG+EMA++QMK
Sbjct: 395 GRMEELWGEDWDEFKPNRWAESYDKTCCRVLKKVNPFKFPVFQAGPRVCLGEEMAYVQMK 454

Query: 444 YVVATVLKRFEFRPVSESPPVFVPLLTAHMAGGLQVYVRER 478
           Y+VA++L RFE  P+    P FVP+LTAHMAGG+QV V  R
Sbjct: 455 YIVASILDRFEIEPIPTDKPDFVPMLTAHMAGGMQVRVHRR 490

BLAST of Sed0010583 vs. TAIR 10
Match: AT2G27690.1 (cytochrome P450, family 94, subfamily C, polypeptide 1 )

HSP 1 Score: 419.9 bits (1078), Expect = 2.9e-117
Identity = 234/489 (47.85%), Postives = 310/489 (63.39%), Query Frame = 0

Query: 7   ISLFFLLCLSLCAFFFLYPFISC---------LVSFHKNRRRLLSWYTDLLSDSSTRTIV 66
           +S FF++  SL    FL     C           S+ K+   L  WYT LL  S T TI 
Sbjct: 10  VSFFFIIIFSLFHLLFLQKLRYCNCEICHAYLTSSWKKDFINLSDWYTHLLRRSPTSTIK 69

Query: 67  VHRLGSRRTIVTANPANVEYMLKTNFVNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 126
           VH L S   ++TANP+NVE++LKTNF NYPKGK F+ ILGDLLG GIFN DGD W  QRK
Sbjct: 70  VHVLNS---VITANPSNVEHILKTNFHNYPKGKQFSVILGDLLGRGIFNSDGDTWRFQRK 129

Query: 127 LASHEFSAKSLREFVVKILEDEVHLRLIPLLQNAARTNG-VLDLQDVLGRLAFDTICKVS 186
           LAS E  + S+R F  +I++ E+  RL+P+L + +   G VLDLQDV  R +FDTI K+S
Sbjct: 130 LASLELGSVSVRVFAHEIVKTEIETRLLPILTSFSDNPGSVLDLQDVFRRFSFDTISKLS 189

Query: 187 LGSDEQCLDMSRPVPAILNAFDTATGISAKRAIAPIFLAWKAKRLFNIGSEKQLKESVRR 246
            G D  CL +  P+     AFDTA+ +SAKRA+AP  L WK KRL  IGSEK+L+ES+  
Sbjct: 190 FGFDPDCLRLPFPISEFAVAFDTASLLSAKRALAPFPLLWKTKRLLRIGSEKKLQESINV 249

Query: 247 VHSWVSDIIRNKNLDTNKPAGDLLSRLLC--AGNDQEVVRDMVVSFIMAGRDTTSAAMTW 306
           ++    D+I+ + L       DL+SR +   A +D E +RD+VVSF++AGRDT +A +T 
Sbjct: 250 INRLAGDLIKQRRLTGLMGKNDLISRFMAVVAEDDDEYLRDIVVSFLLAGRDTVAAGLTG 309

Query: 307 LFWLLTKHPEVQATLMNEVVS-LSNKTASFNYGYEELKDMKYLKACLCESMRLYPPVAWD 366
            FWLLT+HPEV+  +  E+   +     S     +E+++M YL A L ESMRL+PPV +D
Sbjct: 310 FFWLLTRHPEVENRIREELDRVMGTGFDSVTARCDEMREMDYLHASLYESMRLFPPVQFD 369

Query: 367 SKHAAAPDVLPDGTRVRKGDRVTYFPYGMGRMEDLWGKDRLEFKPDRWFQN-GELKPVSP 426
           SK A   DVL DGT V  G RVTY  Y MGRM+ +WG D  EFKP+RW  N G+ +P +P
Sbjct: 370 SKFALNDDVLSDGTFVNSGTRVTYHAYAMGRMDRIWGPDYEEFKPERWLDNEGKFRPENP 429

Query: 427 FKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFEFR---PVSESPPVFVPLLTAHMAGG 479
            K+PVFQAG R+C+GKEMA ++MK +   +++RFE R   P +     F P LTA + GG
Sbjct: 430 VKYPVFQAGARVCIGKEMAIMEMKSIAVAIIRRFETRVASPETTETLRFAPGLTATVNGG 489

BLAST of Sed0010583 vs. TAIR 10
Match: AT1G34540.1 (cytochrome P450, family 94, subfamily D, polypeptide 1 )

HSP 1 Score: 411.8 bits (1057), Expect = 7.8e-115
Identity = 223/492 (45.33%), Postives = 303/492 (61.59%), Query Frame = 0

Query: 11  FLLCLSLCAFFFL-------------YPFISCLVSFHKNRRRLLSWYTDLLSDSSTRTIV 70
           FLLC  +  FF               YP +        NR R L W  + LS   T+T +
Sbjct: 8   FLLCFPISIFFIFFTKKSSSEFGFKSYPIVGSFPGLVNNRHRFLDWTVETLSRCPTQTAI 67

Query: 71  VHRLGSRRTIVTANPANVEYMLKTNFVNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 130
             R G ++ I+TANP+NVEYMLKT F ++PKG+ FT +L D LG GIFN DGD+W  QRK
Sbjct: 68  FRRPGKQQLIMTANPSNVEYMLKTKFESFPKGQQFTSVLEDFLGHGIFNSDGDMWWKQRK 127

Query: 131 LASHEFSAKSLREFVVKILEDEVHLRLIPLLQNAARTNGVLDLQDVLGRLAFDTICKVSL 190
            AS+EFS KSLR+FV+  +  E++ RL+P+L  AA T  ++DLQD+L R AFD ICK++ 
Sbjct: 128 TASYEFSTKSLRDFVMSNVTVEINTRLVPVLVEAATTGKLIDLQDILERFAFDNICKLAF 187

Query: 191 GSDEQCLDMSRPVPA-ILNAFDTATGISAKRAIAPIFLAWKAKRLFNIGSEKQLKESVRR 250
             D  CL     V    + AF+TA  I ++R  +    AW+ K+  NIGSE+ L+ES+  
Sbjct: 188 NVDCACLGHDGAVGVNFMRAFETAATIISQRFRSVASCAWRIKKKLNIGSERVLRESIAT 247

Query: 251 VHSWVSDIIRNKNLDTNKPAG---DLLSRLLCAG--NDQEVVRDMVVSFIMAGRDTTSAA 310
           VH +  +I+RN+ +D  + +    DLLSR +     N  E++RD+V+SFI+AGRDTTS+A
Sbjct: 248 VHKFADEIVRNR-IDQGRSSDHKEDLLSRFISKEEMNSPEILRDIVISFILAGRDTTSSA 307

Query: 311 MTWLFWLLTKHPEVQATLMNEVVSL---SNKTASFNYGYEELKDMKYLKACLCESMRLYP 370
           ++W FWLL+ HPEV+  ++ E+ S+   + K     YG+E LK M YL A + ES+RLYP
Sbjct: 308 LSWFFWLLSMHPEVEDKILQELNSIRARTGKRIGEVYGFEHLKMMNYLHAAITESLRLYP 367

Query: 371 PVAWDSKHAAAPDVLPDGTRVRKGDRVTYFPYGMGRMEDLWGKDRLEFKPDRWFQ--NGE 430
           PV  D K  A  +VLPDGT V KG  +TY  + MGRME +WGKD   F P+RW    NG 
Sbjct: 368 PVPVDIKSCAEDNVLPDGTFVGKGWAITYNIFAMGRMESIWGKDCDRFDPERWIDETNGC 427

Query: 431 LKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVVATVLKRFEFRPVSESPPVFVPLLTAHM 479
            +   P KFP F AGPRMC+GK+MA+IQMK +VA VL+RF      +  P  +  +T  +
Sbjct: 428 FRGEDPSKFPAFHAGPRMCVGKDMAYIQMKSIVAAVLERFVVEVPGKERPEILLSMTLRI 487

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879988.17.8e-23479.34cytochrome P450 94B3-like [Benincasa hispida][more]
XP_022966357.11.1e-23281.96cytochrome P450 94B1-like [Cucurbita maxima][more]
XP_022921449.11.3e-23179.13cytochrome P450 94B3 [Cucurbita moschata][more]
XP_022987517.12.8e-23179.53cytochrome P450 94B3-like [Cucurbita maxima][more]
XP_023516482.11.4e-23079.13cytochrome P450 94B3-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SMP51.7e-17062.66Cytochrome P450 94B3 OS=Arabidopsis thaliana OX=3702 GN=CYP94B3 PE=1 SV=1[more]
Q9FMV72.3e-16461.59Cytochrome P450 94B1 OS=Arabidopsis thaliana OX=3702 GN=CYP94B1 PE=1 SV=1[more]
Q9ZUX14.0e-11647.85Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1[more]
O811171.2e-11244.75Cytochrome P450 94A1 OS=Vicia sativa OX=3908 GN=CYP94A1 PE=2 SV=2[more]
P981886.3e-10944.68Cytochrome P450 94A2 OS=Vicia sativa OX=3908 GN=CYP94A2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HMR45.5e-23381.96cytochrome P450 94B1-like OS=Cucurbita maxima OX=3661 GN=LOC111466028 PE=4 SV=1[more]
A0A6J1E0I06.1e-23279.13cytochrome P450 94B3 OS=Cucurbita moschata OX=3662 GN=LOC111429724 PE=3 SV=1[more]
A0A6J1JAK91.3e-23179.53cytochrome P450 94B3-like OS=Cucurbita maxima OX=3661 GN=LOC111485059 PE=3 SV=1[more]
A0A6J1F5332.8e-22979.96cytochrome P450 94B3-like OS=Cucurbita moschata OX=3662 GN=LOC111440917 PE=4 SV=... [more]
A0A6J1C2528.5e-22677.89cytochrome P450 94B3-like OS=Momordica charantia OX=3673 GN=LOC111007253 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT3G48520.11.2e-17162.66cytochrome P450, family 94, subfamily B, polypeptide 3 [more]
AT5G63450.11.7e-16561.59cytochrome P450, family 94, subfamily B, polypeptide 1 [more]
AT3G01900.11.2e-15258.13cytochrome P450, family 94, subfamily B, polypeptide 2 [more]
AT2G27690.12.9e-11747.85cytochrome P450, family 94, subfamily C, polypeptide 1 [more]
AT1G34540.17.8e-11545.33cytochrome P450, family 94, subfamily D, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 425..448
score: 25.59
coord: 272..289
score: 29.45
coord: 292..318
score: 25.9
coord: 415..425
score: 44.93
IPR001128Cytochrome P450PRINTSPR00385P450coord: 283..300
score: 36.98
coord: 416..425
score: 46.46
coord: 338..349
score: 38.04
coord: 425..436
score: 32.94
IPR001128Cytochrome P450PFAMPF00067p450coord: 54..456
e-value: 2.5E-62
score: 211.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 20..484
e-value: 1.4E-94
score: 319.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 17..477
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 19..475
NoneNo IPR availablePANTHERPTHR24296:SF5CYTOCHROME P450 94B3coord: 19..475

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0010583.1Sed0010583.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen