Sed0009648 (gene) Chayote v1

Overview


Name	Sed0009648
Type	gene
Organism	Sechium edule (Chayote v1)
Description	Calcium-binding EF-hand
Location	LG10: 27652820 .. 27653458 (+)
RNA-Seq Expression	Sed0009648
Synteny	Sed0009648

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAACACAAAGCAACTTGGGTGGGAATTTGTTCTTGTTTGTGTGCATGGGAAATCAGTACAGTTCGTTGAGTGAAGAAGAGATTGCCTTGGTTTTCAAGCAACACGATGGGGACAAAGACAGGAAGCTGAGCAAAGAAGAGTTGAAGAGGGCTTTCAAGTACTTGAATTCGAGGTGTAGCGCCTTCCGAGCCAACGCAGCACTTCGTGCGGTCGATACTGACAGAGATGGCCTCATCAGTTCCGACGAGATGCGTAATCTCATTTTGTATGTTAAAGGCCTTCGTTGATAAAACTGCACTCTTCTACATACACTTATAGTATGTATTTGAAGTGAAACAAAAACCTTTATTGTCCAAAAATAATACCTGGAATGTCGACCTTCTAAATTACGTCGTAGGTCTGAATATTTATCTTTAAGTTTTTTTTTTAATAAGAATGCTTATCTTTAAGTTGGGGCGTTATGTTTTAGTAAAACTAAAAGTATTGTTATGTATTTTCCTCTCTTGTGTGTCTATTAGAGTATGCTTGATAATAACTTTTCTGCTATATCCTATAATTTTTTTTTTTTTAATCAAGAAAAGTATATGTGAAATTCTAATTAGATTTCAAAACAACCACAAAGAGGTATCCATTTCCAC

mRNA sequence

AAAACACAAAGCAACTTGGGTGGGAATTTGTTCTTGTTTGTGTGCATGGGAAATCAGTACAGTTCGTTGAGTGAAGAAGAGATTGCCTTGGTTTTCAAGCAACACGATGGGGACAAAGACAGGAAGCTGAGCAAAGAAGAGTTGAAGAGGGCTTTCAAGTACTTGAATTCGAGGTGTAGCGCCTTCCGAGCCAACGCAGCACTTCGTGCGGTCGATACTGACAGAGATGGCCTCATCAGTTCCGACGAGATGCGTAATCTCATTTTGTATGTTAAAGGCCTTCGTTGATAAAACTGCACTCTTCTACATACACTTATAGTATGTATTTGAAGTGAAACAAAAACCTTTATTGTCCAAAAATAATACCTGGAATGTCGACCTTCTAAATTACGTCGTAGGTCTGAATATTTATCTTTAAGTTTTTTTTTTAATAAGAATGCTTATCTTTAAGTTGGGGCGTTATGTTTTAGTAAAACTAAAAGTATTGTTATGTATTTTCCTCTCTTGTGTGTCTATTAGAGTATGCTTGATAATAACTTTTCTGCTATATCCTATAATTTTTTTTTTTTTAATCAAGAAAAGTATATGTGAAATTCTAATTAGATTTCAAAACAACCACAAAGAGGTATCCATTTCCAC

Coding sequence (CDS)

ATGGGAAATCAGTACAGTTCGTTGAGTGAAGAAGAGATTGCCTTGGTTTTCAAGCAACACGATGGGGACAAAGACAGGAAGCTGAGCAAAGAAGAGTTGAAGAGGGCTTTCAAGTACTTGAATTCGAGGTGTAGCGCCTTCCGAGCCAACGCAGCACTTCGTGCGGTCGATACTGACAGAGATGGCCTCATCAGTTCCGACGAGATGCGTAATCTCATTTTGTATGTTAAAGGCCTTCGTTGA

Protein sequence

MGNQYSSLSEEEIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDRDGLISSDEMRNLILYVKGLR

Homology

[Close]

BLAST of Sed0009648 vs. NCBI nr
Match: KAA0060897.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 105.9 bits (263), Expect = 1.6e-19
Identity = 54/80 (67.50%), Postives = 65/80 (81.25%), Query Frame = 0


Query: 1   MGNQYSSLSEEEIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDR 60
           MGN+YSSLSEE++A +FK+HD D+DRKL+KEEL RAF YL SR +A R   ALRAVD + 
Sbjct: 35  MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELARAFDYLGSRWTARRVKGALRAVDANG 94

Query: 61  DGLISSDEMRNLILYVKGLR 81
           DG+IS DEM  LI+YVKGLR
Sbjct: 95  DGVISMDEMSKLIVYVKGLR 114

[Close]

BLAST of Sed0009648 vs. NCBI nr
Match: KAE8652092.1 (hypothetical protein Csa_021918 [Cucumis sativus])

HSP 1 Score: 104.8 bits (260), Expect = 3.6e-19
Identity = 52/80 (65.00%), Postives = 64/80 (80.00%), Query Frame = 0


Query: 1   MGNQYSSLSEEEIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDR 60
           MGN+YSSLSEE++A +FK+HD D+DRKL+KEEL RAF YL SR +A R   A+RAVD + 
Sbjct: 36  MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANH 95

Query: 61  DGLISSDEMRNLILYVKGLR 81
           DG+IS DEM  LI+YVKG R
Sbjct: 96  DGVISLDEMSKLIVYVKGCR 115

[Close]

BLAST of Sed0009648 vs. NCBI nr
Match: KAG7020566.1 (hypothetical protein SDJN02_17252, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 88.2 bits (217), Expect = 3.5e-14
Identity = 45/71 (63.38%), Postives = 52/71 (73.24%), Query Frame = 0


Query: 9   SEEEIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDRDGLISSDE 68
           +EE++A +FK HD D D KLSKEELK+AF YL SR S+FR   ALRA DT+ DG IS DE
Sbjct: 52  TEEQLAAIFKSHDRDGDGKLSKEELKQAFNYLGSRFSSFRVKEALRAADTNGDGFISMDE 111

Query: 69  MRNLILYVKGL 80
           M  LI Y KGL
Sbjct: 112 MNKLIKYTKGL 122

[Close]

BLAST of Sed0009648 vs. NCBI nr
Match: KAG7020569.1 (hypothetical protein SDJN02_17255, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 86.7 bits (213), Expect = 1.0e-13
Identity = 44/70 (62.86%), Postives = 51/70 (72.86%), Query Frame = 0


Query: 9  SEEEIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDRDGLISSDE 68
          +EE++A +FK HD D D KLSKEELK+AF YL SR S+FR   ALRA DT+ DG IS DE
Sbjct: 17 TEEQLAAIFKSHDRDGDGKLSKEELKQAFNYLGSRFSSFRVKEALRAADTNGDGFISMDE 76

Query: 69 MRNLILYVKG 79
          M  LI Y KG
Sbjct: 77 MNKLIKYTKG 86

[Close]

BLAST of Sed0009648 vs. NCBI nr
Match: KAG6585661.1 (putative calcium-binding protein CML15, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020568.1 putative calcium-binding protein CML15, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 86.3 bits (212), Expect = 1.3e-13
Identity = 44/71 (61.97%), Postives = 52/71 (73.24%), Query Frame = 0


Query: 8  LSEEEIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDRDGLISSD 67
          +SEE++  +FK HD D D KLSKEELK+AF YL SR SAFRA  ALR VD + DG++S D
Sbjct: 16 VSEEQLLDIFKSHDRDGDGKLSKEELKQAFSYLGSRFSAFRAEEALRTVDDNHDGVVSMD 75

Query: 68 EMRNLILYVKG 79
          EM  LI Y KG
Sbjct: 76 EMNKLIQYTKG 86

[Close]

BLAST of Sed0009648 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 4.5e-04
Identity = 24/62 (38.71%), Postives = 37/62 (59.68%), Query Frame = 0


Query: 12  EIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDRDGLISSDEMRN 71
           E+  VF   D ++D ++S EEL+++F  L  + S   A AA+R  DTD DG++  +E   
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 72  LI 74
           LI
Sbjct: 103 LI 104

[Close]

BLAST of Sed0009648 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 5.8e-04
Identity = 26/62 (41.94%), Postives = 33/62 (53.23%), Query Frame = 0


Query: 11 EEIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDRDGLISSDEMR 70
          EE+  VF Q D + D K+S  EL   FK + +  +    N  L  VDTDRDG I+ DE  
Sbjct: 22 EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 71 NL 73
           L
Sbjct: 82 TL 83

[Close]

BLAST of Sed0009648 vs. ExPASy TrEMBL
Match: A0A5A7V219 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00300 PE=4 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 7.9e-20
Identity = 54/80 (67.50%), Postives = 65/80 (81.25%), Query Frame = 0


Query: 1   MGNQYSSLSEEEIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDR 60
           MGN+YSSLSEE++A +FK+HD D+DRKL+KEEL RAF YL SR +A R   ALRAVD + 
Sbjct: 35  MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELARAFDYLGSRWTARRVKGALRAVDANG 94

Query: 61  DGLISSDEMRNLILYVKGLR 81
           DG+IS DEM  LI+YVKGLR
Sbjct: 95  DGVISMDEMSKLIVYVKGLR 114

[Close]

BLAST of Sed0009648 vs. ExPASy TrEMBL
Match: A0A0A0LL93 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357850 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.8e-19
Identity = 52/80 (65.00%), Postives = 64/80 (80.00%), Query Frame = 0


Query: 1  MGNQYSSLSEEEIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDR 60
          MGN+YSSLSEE++A +FK+HD D+DRKL+KEEL RAF YL SR +A R   A+RAVD + 
Sbjct: 1  MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANH 60

Query: 61 DGLISSDEMRNLILYVKGLR 81
          DG+IS DEM  LI+YVKG R
Sbjct: 61 DGVISLDEMSKLIVYVKGCR 80

[Close]

BLAST of Sed0009648 vs. ExPASy TrEMBL
Match: A0A5A7V5B0 (Putative Calcium-binding EF-hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00320 PE=4 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.7e-12
Identity = 43/69 (62.32%), Postives = 50/69 (72.46%), Query Frame = 0


Query: 9  SEEEIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDRDGLISSDE 68
          SEE++A VFK HD D D  L+KEELK+AF+YL SR S FR   ALRA DT+ DG+IS DE
Sbjct: 17 SEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFSNFRVEEALRAADTNGDGVISMDE 76

Query: 69 MRNLILYVK 78
          M  LI Y K
Sbjct: 77 MGKLIKYAK 85

[Close]

BLAST of Sed0009648 vs. ExPASy TrEMBL
Match: A0A0A0LRB6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357340 PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.3e-11
Identity = 42/69 (60.87%), Postives = 49/69 (71.01%), Query Frame = 0


Query: 9  SEEEIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDRDGLISSDE 68
          SEE++A VFK HD D D  L+KEELK+AF+YL SR   FR   ALRA DT+ DG+IS DE
Sbjct: 17 SEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVISMDE 76

Query: 69 MRNLILYVK 78
          M  LI Y K
Sbjct: 77 MGKLIKYAK 85

[Close]

BLAST of Sed0009648 vs. ExPASy TrEMBL
Match: A0A0A0LP43 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357350 PE=4 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 7.4e-10
Identity = 38/73 (52.05%), Postives = 50/73 (68.49%), Query Frame = 0


Query: 6  SSLSEEEIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDRDGLIS 65
          +SL+E E+A VFK HD D D +L+KEELK AFKYL  + + FR + AL A D + D +IS
Sbjct: 14 ASLTEFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVIS 73

Query: 66 SDEMRNLILYVKG 79
           +EM  LI+Y  G
Sbjct: 74 FEEMSKLIMYTAG 86

[Close]

BLAST of Sed0009648 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 45.1 bits (105), Expect = 3.2e-05
Identity = 24/62 (38.71%), Postives = 37/62 (59.68%), Query Frame = 0


Query: 12  EIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDRDGLISSDEMRN 71
           E+  VF   D ++D ++S EEL+++F  L  + S   A AA+R  DTD DG++  +E   
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 72  LI 74
           LI
Sbjct: 103 LI 104

[Close]

BLAST of Sed0009648 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 45.1 bits (105), Expect = 3.2e-05
Identity = 24/62 (38.71%), Postives = 37/62 (59.68%), Query Frame = 0


Query: 12  EIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDRDGLISSDEMRN 71
           E+  VF   D ++D ++S EEL+++F  L  + S   A AA+R  DTD DG++  +E   
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 72  LI 74
           LI
Sbjct: 103 LI 104

[Close]

BLAST of Sed0009648 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 44.7 bits (104), Expect = 4.1e-05
Identity = 26/62 (41.94%), Postives = 33/62 (53.23%), Query Frame = 0


Query: 11 EEIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDRDGLISSDEMR 70
          EE+  VF Q D + D K+S  EL   FK + +  +    N  L  VDTDRDG I+ DE  
Sbjct: 22 EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 71 NL 73
           L
Sbjct: 82 TL 83

[Close]

BLAST of Sed0009648 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 44.7 bits (104), Expect = 4.1e-05
Identity = 26/62 (41.94%), Postives = 33/62 (53.23%), Query Frame = 0


Query: 11 EEIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDRDGLISSDEMR 70
          EE+  VF Q D + D K+S  EL   FK + +  +    N  L  VDTDRDG I+ DE  
Sbjct: 22 EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 71 NL 73
           L
Sbjct: 82 TL 83

[Close]

BLAST of Sed0009648 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 43.5 bits (101), Expect = 9.2e-05
Identity = 23/62 (37.10%), Postives = 37/62 (59.68%), Query Frame = 0


Query: 12 EIALVFKQHDGDKDRKLSKEELKRAFKYLNSRCSAFRANAALRAVDTDRDGLISSDEMRN 71
          ++  VF   D ++D ++S EELK++FK L  + S   A AA++  D D DG++  +E   
Sbjct: 22 DLEAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFAL 81

Query: 72 LI 74
          LI
Sbjct: 82 LI 83

The following BLAST results are available for this feature:

BLAST of Sed0009648 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0060897.1	1.6e-19	67.50	putative calcium-binding protein CML10 [Cucumis melo var. makuwa]	[more]
KAE8652092.1	3.6e-19	65.00	hypothetical protein Csa_021918 [Cucumis sativus]	[more]
KAG7020566.1	3.5e-14	63.38	hypothetical protein SDJN02_17252, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG7020569.1	1.0e-13	62.86	hypothetical protein SDJN02_17255, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6585661.1	1.3e-13	61.97	putative calcium-binding protein CML15, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of Sed0009648 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRE6	4.5e-04	38.71	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]
Q9LE22	5.8e-04	41.94	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]

BLAST of Sed0009648 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7V219	7.9e-20	67.50	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0LL93	1.8e-19	65.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357850 PE=4 SV=1	[more]
A0A5A7V5B0	2.7e-12	62.32	Putative Calcium-binding EF-hand family protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A0A0LRB6	1.3e-11	60.87	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357340 PE=4 SV=1	[more]
A0A0A0LP43	7.4e-10	52.05	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357350 PE=4 SV=1	[more]

BLAST of Sed0009648 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G76650.1	3.2e-05	38.71	calmodulin-like 38	[more]
AT1G76650.3	3.2e-05	38.71	calmodulin-like 38	[more]
AT1G18210.1	4.1e-05	41.94	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	4.1e-05	41.94	Calcium-binding EF-hand family protein	[more]
AT1G76640.1	9.2e-05	37.10	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 12..40 e-value: 1.5 score: 16.2 coord: 48..76 e-value: 3.4 score: 13.5
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 11..72 e-value: 2.5E-9 score: 37.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 44..79 score: 9.93856
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 8..43 score: 12.337564
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 12..73 e-value: 1.09656E-8 score: 44.8461
None	No IPR available	GENE3D	1.10.238.10		coord: 2..76 e-value: 1.8E-13 score: 52.8
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 57..69
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 21..33
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 7..74

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0009648.1	Sed0009648.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding