Sed0009225 (gene) Chayote v1

Overview
NameSed0009225
Typegene
OrganismSechium edule (Chayote v1)
DescriptionCytochrome p450
LocationLG09: 40232290 .. 40236337 (+)
RNA-Seq ExpressionSed0009225
SyntenySed0009225
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAGTTTATGGGATGTTGTGGTAGTGACAACCATCTTTTTGACCACCATTATTCTCTATAGGAATTGCTTTAGACTTTTCAGATCAAAATTCTGCAACCAACTTCCTTTAGGCTCTCTTGGCTGGCCTTTCATTGGTGAGACTATTGAGTTCATTGCTTGTGCTTATTCCGACCGCCCTGAAACCTTCATGGACAAGCGTCGCCGACTGTAAGTAAATCCACATAAACCAACTTCATAACATCTGAATTCCTTCTAATGCTGATCTGACTTGTGATTGTTTGTTAATTGGAGTTGCTTGGTTTTCTTATGAACCAATGTTTTATTTTGGTTTGAAGGTATGGGAAGGTGTTCAAGTCACATATTTTTGGAAGCCCTACCATTGTTTCTACTGATGCAGAAGTCAGTAAGTTCATTCTGCAGTGTGATGCCAAGGCTTTCATACCATCTTACCCGAAATCCTTGACGAAGTTGATGGGGAGATCTTCTATTTTGCTGATAAATGGGAGCTTGCAGAAGAGAATTCATGGTCTTGTTGGAGCTTTCTTCAAGTCTTCACTGCTTAAAGCTCAAATAACCAAGGATATGGAGTGTTATGTCAAACTGTCAATGGAAAATTGGACTGAAACCAATCCTATTTACCTTCAAGATGAAACCAAAAATGTCAGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATATATATATATATATATATATCTCCCCTTTTTGAAGCTTGAAACAAAGGTCGTGTTTGGGGCACTAGCTGGGCTATCATAAACGGTAGATTATTATAAACGATGAGTTATTATAAATTGTGTTTAAGGTGCAGGTTATTATAACATGAGTTATGATAAAGTATAAATGGTGTTCGGGGTAAGGTAATTACAACAAGAGTTATAATAAGTAGTGTTTGGAGTGCACGTAACTGTAACAGGGAAGAAAAAATGGTTAATCTCAAATGAGAGCATGAATTATGTGAAACGACAGCTTGAGTTTGGGCCAACTCATTGACTATTATAACGGGTGAGCCAAACGTGGGTTGAGTTATAATACCCCAACTGTCCGCTTGCATAAACAGCTCCCAAACACGACCTAACCTGTGAACTCTCCTTTTCTTCTTCCCTTGCATGAAGTTAACCAAAAGACAATAGATTAAAATTTCTACCATTACTCTGTTGATACTAGACAAAAACAGTGACTTATCTTAACTGATTTGAGTGAATCATTGATGCCCAAATAATCCATTTCTCTAGCTCCTACTTGGGTTTAAAGATGATTTTTTTTAATCAAAAGTAACAGAACCCCACAGTTCTATCATCATTCTGTTAAAATCATAGTGTCCAAGAATTTACCTTTGTAATCATTTCAGCTATATATTTTCCTGTTCAAAAAGTTACTAAGTGAGCCCATTTTGTCTCCAGATTGCCTTTCAAGTATTAGTAAAGATATTGATTAGTTTAAATCCTGGTCAAAGGATGGAGTTTCTCAAGCAACAGTTTCAAGAATTCATCGCGGGGCTCATGGCTTTACCGGTGATCATACCCGGAAGCACGCTTTACCGATCGTTGCAGGCAAGTTGGTCGTAAAAGGTTTAATATCCTCCTTACTAGTACTTCTTTACTGCATTCTAAAAAATGATGGAGCTTCATTTTGTTATTGAATTTCAGGCCAAAAGGATTATGGTTGAACTTGTAAAAAAGATAATACAAGAAAAAAAAGGCAGTGGAATTTCCCCTGTCCCAAAAGATGTGATGGAAGTTTTGCTCAAAGATGAAAGTGGACAACTAACAGATGATCTAATAGCAGGGAACATGATTGACATGATGATTCCTGGGGAAGATTCAGTGCCAATCTTAATGACTCTTGCAGTAAAATACCTCTCCGATTGCCCTGCCGCACTTCGACAATTAACGGTATGCTATTATTCGTGCTAACTCTCAACGTTCTTAGTTCTTTGACAGTATTTTGACGATCTCCCTCACTTGTGTCCTTGAAAATTTTGTAGAAGTCTCAACAAGTGGTTTTTAATGTTAATGGAGGAAATAATATTAAGGAGTTTTGAATACAAGTTCTATATATCGAATCATCAACCAAACCAAAAGCCTAAGCTGATAAGTTTATTTATTTTTATCAATACTTTAATAATATCATCGCGGAAAACTTTGCTTCTATGAGATACAAATATGGTCATGGACTTTATTTAGTCATCCTTTCTATCACTTGGGAAAGCAAATATGCTTATTTTCATGTTCACTTTAACAAATGTACCTATCAACTATGTTCCTTTTATACCAGTCACATGGTCCTCAGGTGTGCTAGCTTGAGCGACTCCAGACCGACTTCCTAAAGTACTAATGGGGTATTTGGGGGAAGGTGGATAAAAATAATTATGAGGAGAATGATTCTCTTACCCACGTTTGGGCCACCGAGAATCATTCTCCTCATAATCTCATTTCTCAATAACTACTTTCTATTCTACTCATAACTACTTCATATTTTCTCCTCATAACCACTTTTCTTTCTTCATATAATCAGTTTTTATTCTCTTCATACTCGCTATTTTATTCTCCTTAAAATTATTCTTTTCACCCCAAACACAAGTAATGACTCTCATAATCATTATATTCCCCCTAAACATAAATAATTATAATCATTCTCCCTCGTATTCTTCTCCTAATTTTCATCCTCTCCTTTATCATTCTCATTCTCCTTGTTTCGAACACTTCTAGTTAGGAAAGCTCATATCTGAATCAATTTTAGGGAAACCATAGCTTGGATATAAGTTCAATGTACACATCTTGTCTATTTAGTAACACTAAGTAAAACAGTTAAATCTATGCTCTTCCTTGCCTTCTTTGAATATTTCCTAAACTTTATGCCTGTTTGGACCATATCAGGAGGAAAACTTGAAGATGAAAAAAGTCAAAGAGCAGCTCGGAGAGCCGTTGCATTGGAGCGATTACTTATCGTCGCTCCCATTTACGCAAAGTGTAATACATTAACCTTTTTGAAAAAAGTCAAAAGCTAGAGTGCTTGGAAGTTATATTGAATGTGCTTTTCATTTGAATAGGTGATCACGGAGACGCTACGGATGGGAAACATCATAATTGGGGTGATGAGGAAGGCCATGAAAGACATGAAGATAAAAGGATACAGAATACCGAAAGGTTGGTCGGTACTTACGTACTTTAGATCTGTTCATCTCGACGACAGTCTCTATGAATGGCCTTACAAATTCAATCCATGGAGGTGGCAAGTAAGAAAAGAACATATTTCTCACTTTTTTTTTTTAATACTAGTTTGTCTGGATTAGCTTTTGCACGCCTCGACTATTATCACGGGACATACAGTCTGATCGTACAATATTTTTGCTTTTGATGTTTGTCTCTCCCTAATGGATTTCTGGTCCAACAGGAGAGAGACTTGAGTAGTTGTAACTTCACTCCTTTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTGGCTAGGCTTGAAGCTTCCATTTTCCTTCATCACTTTGTCACTCAGTTCAGGTAACTACCAAATTCACTTTTACCTCTAAAACATTCTTTTTTATTGTTTTTCTTGTTTCAATTGAAATGAATATAAAGCCACTCAAAAAAAATTGTGCCATATTGATCCCTTAGGCATCTGATTTGGTATCTTTATGTTTTCTTCCCAACTAGAATGTCGGGAACTCCCCTCGGTCTCGATCGTTACATTATGCTCCTGGCCCGCCCTACTATTCTGCAAAAAGATAGACTAAGCCTTAAGATTTGAACTCCTGACTTCTTGGTCATACACTTATATTGTTGAACTATTACTTTTGTTGACACATTCTCTCTTGTTTCTATGCACTTTCTTCGAGTGCGAACATGAAGATTGTTTGGAATACTTTTCTAAAAAAACCAAATGTGGTATATTACAGATGGATTGCTGAGGAAGATAGAATCATAAACTTCCCCACAGTGAGGATGAAGAAGAGAATGCCAGTTTGGGTGAAGAGAATAAGGGACCCATGATTGTGG

mRNA sequence

ATGGACAGTTTATGGGATGTTGTGGTAGTGACAACCATCTTTTTGACCACCATTATTCTCTATAGGAATTGCTTTAGACTTTTCAGATCAAAATTCTGCAACCAACTTCCTTTAGGCTCTCTTGGCTGGCCTTTCATTGGTGAGACTATTGAGTTCATTGCTTGTGCTTATTCCGACCGCCCTGAAACCTTCATGGACAAGCGTCGCCGACTGTATGGGAAGGTGTTCAAGTCACATATTTTTGGAAGCCCTACCATTGTTTCTACTGATGCAGAAGTCAGTAAGTTCATTCTGCAGTGTGATGCCAAGGCTTTCATACCATCTTACCCGAAATCCTTGACGAAGTTGATGGGGAGATCTTCTATTTTGCTGATAAATGGGAGCTTGCAGAAGAGAATTCATGGTCTTGTTGGAGCTTTCTTCAAGTCTTCACTGCTTAAAGCTCAAATAACCAAGGATATGGAGTGTTATGTCAAACTGTCAATGGAAAATTGGACTGAAACCAATCCTATTTACCTTCAAGATGAAACCAAAAATATTGCCTTTCAAGTATTAGTAAAGATATTGATTAGTTTAAATCCTGGTCAAAGGATGGAGTTTCTCAAGCAACAGTTTCAAGAATTCATCGCGGGGCTCATGGCTTTACCGGTGATCATACCCGGAAGCACGCTTTACCGATCGTTGCAGGCCAAAAGGATTATGGTTGAACTTGTAAAAAAGATAATACAAGAAAAAAAAGGCAGTGGAATTTCCCCTGTCCCAAAAGATGTGATGGAAGTTTTGCTCAAAGATGAAAGTGGACAACTAACAGATGATCTAATAGCAGGGAACATGATTGACATGATGATTCCTGGGGAAGATTCAGTGCCAATCTTAATGACTCTTGCAGTAAAATACCTCTCCGATTGCCCTGCCGCACTTCGACAATTAACGGAGGAAAACTTGAAGATGAAAAAAGTCAAAGAGCAGCTCGGAGAGCCGTTGCATTGGAGCGATTACTTATCGTCGCTCCCATTTACGCAAAGTGTGATCACGGAGACGCTACGGATGGGAAACATCATAATTGGGGTGATGAGGAAGGCCATGAAAGACATGAAGATAAAAGGATACAGAATACCGAAAGGTTGGTCGGTACTTACGTACTTTAGATCTGTTCATCTCGACGACAGTCTCTATGAATGGCCTTACAAATTCAATCCATGGAGGTGGCAAGAGAGAGACTTGAGTAGTTGTAACTTCACTCCTTTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTGGCTAGGCTTGAAGCTTCCATTTTCCTTCATCACTTTGTCACTCAGTTCAGATGGATTGCTGAGGAAGATAGAATCATAAACTTCCCCACAGTGAGGATGAAGAAGAGAATGCCAGTTTGGGTGAAGAGAATAAGGGACCCATGATTGTGG

Coding sequence (CDS)

ATGGACAGTTTATGGGATGTTGTGGTAGTGACAACCATCTTTTTGACCACCATTATTCTCTATAGGAATTGCTTTAGACTTTTCAGATCAAAATTCTGCAACCAACTTCCTTTAGGCTCTCTTGGCTGGCCTTTCATTGGTGAGACTATTGAGTTCATTGCTTGTGCTTATTCCGACCGCCCTGAAACCTTCATGGACAAGCGTCGCCGACTGTATGGGAAGGTGTTCAAGTCACATATTTTTGGAAGCCCTACCATTGTTTCTACTGATGCAGAAGTCAGTAAGTTCATTCTGCAGTGTGATGCCAAGGCTTTCATACCATCTTACCCGAAATCCTTGACGAAGTTGATGGGGAGATCTTCTATTTTGCTGATAAATGGGAGCTTGCAGAAGAGAATTCATGGTCTTGTTGGAGCTTTCTTCAAGTCTTCACTGCTTAAAGCTCAAATAACCAAGGATATGGAGTGTTATGTCAAACTGTCAATGGAAAATTGGACTGAAACCAATCCTATTTACCTTCAAGATGAAACCAAAAATATTGCCTTTCAAGTATTAGTAAAGATATTGATTAGTTTAAATCCTGGTCAAAGGATGGAGTTTCTCAAGCAACAGTTTCAAGAATTCATCGCGGGGCTCATGGCTTTACCGGTGATCATACCCGGAAGCACGCTTTACCGATCGTTGCAGGCCAAAAGGATTATGGTTGAACTTGTAAAAAAGATAATACAAGAAAAAAAAGGCAGTGGAATTTCCCCTGTCCCAAAAGATGTGATGGAAGTTTTGCTCAAAGATGAAAGTGGACAACTAACAGATGATCTAATAGCAGGGAACATGATTGACATGATGATTCCTGGGGAAGATTCAGTGCCAATCTTAATGACTCTTGCAGTAAAATACCTCTCCGATTGCCCTGCCGCACTTCGACAATTAACGGAGGAAAACTTGAAGATGAAAAAAGTCAAAGAGCAGCTCGGAGAGCCGTTGCATTGGAGCGATTACTTATCGTCGCTCCCATTTACGCAAAGTGTGATCACGGAGACGCTACGGATGGGAAACATCATAATTGGGGTGATGAGGAAGGCCATGAAAGACATGAAGATAAAAGGATACAGAATACCGAAAGGTTGGTCGGTACTTACGTACTTTAGATCTGTTCATCTCGACGACAGTCTCTATGAATGGCCTTACAAATTCAATCCATGGAGGTGGCAAGAGAGAGACTTGAGTAGTTGTAACTTCACTCCTTTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTGGCTAGGCTTGAAGCTTCCATTTTCCTTCATCACTTTGTCACTCAGTTCAGATGGATTGCTGAGGAAGATAGAATCATAAACTTCCCCACAGTGAGGATGAAGAAGAGAATGCCAGTTTGGGTGAAGAGAATAAGGGACCCATGA

Protein sequence

MDSLWDVVVVTTIFLTTIILYRNCFRLFRSKFCNQLPLGSLGWPFIGETIEFIACAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQCDAKAFIPSYPKSLTKLMGRSSILLINGSLQKRIHGLVGAFFKSSLLKAQITKDMECYVKLSMENWTETNPIYLQDETKNIAFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVIIPGSTLYRSLQAKRIMVELVKKIIQEKKGSGISPVPKDVMEVLLKDESGQLTDDLIAGNMIDMMIPGEDSVPILMTLAVKYLSDCPAALRQLTEENLKMKKVKEQLGEPLHWSDYLSSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYRIPKGWSVLTYFRSVHLDDSLYEWPYKFNPWRWQERDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPVWVKRIRDP
Homology
BLAST of Sed0009225 vs. NCBI nr
Match: XP_008462361.1 (PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo])

HSP 1 Score: 852.0 bits (2200), Expect = 2.4e-243
Identity = 428/475 (90.11%), Postives = 447/475 (94.11%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFLTTIILYRNCFRLFRSKFCNQLPLGSLGWPFIGETIEFIACAYSDR 60
           MDSLWDV+VVT I   TIILYRNCFRL RSKFCNQLPLGSLGWPFIGETIEFI+CAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQCDAKAFIPSYPKSLTKLMGRS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQ DAKAF+PSYPKSLT+LMG+S
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSLLKAQITKDMECYVKLSMENWTETNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKAQITKDMECYVKLSM +WTE NPIYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 AFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVIIPGSTLYRSLQAKRIMVELVKK 240
           +FQVLVKILISLNPGQ MEFLK+QFQEFIAGLMALPV IPGS LY+SLQAKR MV+LVKK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQEKKGSGISPVPKDVMEVLLKDESGQLTDDLIAGNMIDMMIPGEDSVPILMTLAVKYL 300
           IIQE+K  GIS VPKDVMEVLLKDE+ +LTDDLIA NMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALRQLTEENLKMKKVKEQLGEPLHWSDYLSSLPFTQSVITETLRMGNIIIGVMRK 360
           SDCPAAL+QLTEENLKMK  KEQLGE LHWSDYL S+PFTQSVITETLRMGNIIIGVMRK
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYL-SMPFTQSVITETLRMGNIIIGVMRK 360

Query: 361 AMKDMKIKGYRIPKGWSVLTYFRSVHLDDSLYEWPYKFNPWRWQERDLSSCNFTPFGGGQ 420
           AMKDMKIKGY I KGWSVLTYFRSVHLDD+ Y+ PYKFNPWRWQER+LSSCNFTPFGGGQ
Sbjct: 361 AMKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQ 420

Query: 421 RLCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPVWVKRIRDP 476
           RLCPGLDLARLEASIFLHHFVT FRWIAEED+IINFPTVRMKKRMPVWVKR RDP
Sbjct: 421 RLCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP 471

BLAST of Sed0009225 vs. NCBI nr
Match: KAG6575481.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 820.8 bits (2119), Expect = 5.9e-234
Identity = 412/505 (81.58%), Postives = 442/505 (87.52%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFLTTIILYRNCFRLFRSKFCNQLPLGSLGWPFIGETIEFIACAYSDR 60
           MDS WDV+V+T IFLT I+LYRNCF L RSK C+ LPLGSLGWPFIGET+EFIACAYSDR
Sbjct: 1   MDSFWDVLVLTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQCDAKAFIPSYPKSLTKLMGRS 120
           PE FMDKRRRLYGKVFKSH+FGSPTIVSTDAEVSKFILQ DAKAF+P YPKSLT+LMG+S
Sbjct: 61  PEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSLLKAQITKDMECYVKLSMENWTETNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKAQITKDMECYVKL+M NW+E +P+YLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNI 180

Query: 181 AFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVIIPGSTLYRS------------- 240
           +FQVLVKILISLNPGQRMEF+KQQFQEFIAGLMALPV IPGS LY+S             
Sbjct: 181 SFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALPSS 240

Query: 241 ---------------LQAKRIMVELVKKIIQEKKGSGISP--VPKDVMEVLLKDESGQLT 300
                          LQAKR MV+LVKKIIQE++  G SP  VP+DVMEVLLKDES  LT
Sbjct: 241 FTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDESELLT 300

Query: 301 DDLIAGNMIDMMIPGEDSVPILMTLAVKYLSDCPAALRQLTEENLKMKKVKEQLGEPLHW 360
           DDLIA NMIDMMIPGEDSVPILMTLAVKYLSDCP AL+QLTEENLKMK++KEQ GE LHW
Sbjct: 301 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEALHW 360

Query: 361 SDYLSSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYRIPKGWSVLTYFRSVHLDDS 420
           SDYL S+PFTQ+VITETLRMGNIIIGVMRKAMKDM IKGY IPKGWSVLTYFRSVHLDDS
Sbjct: 361 SDYL-SMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDS 420

Query: 421 LYEWPYKFNPWRWQERDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIAEE 476
            Y  PYKFNPWRWQ+RDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVT+FRW+AEE
Sbjct: 421 HYHCPYKFNPWRWQDRDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTRFRWVAEE 480

BLAST of Sed0009225 vs. NCBI nr
Match: XP_038898401.1 (LOW QUALITY PROTEIN: cytochrome P450 90D2-like [Benincasa hispida])

HSP 1 Score: 807.4 bits (2084), Expect = 6.7e-230
Identity = 404/461 (87.64%), Postives = 431/461 (93.49%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFLTTIILYRNCFRLFRSKFCNQLPLGSLGWPFIGETIEFIACAYSDR 60
           MDSLWDV+VVT IF TTIILYRNCFRL RSKFCNQLPLGSLGWPFIGETIEFI+CAYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQCDAKAFIPSYPKSLTKLMGRS 120
           PETFM+KRRRLYGKVFKSHIFGSPTIVSTD+EVSKFILQ DAKAF+PSYPKSLT+LMG+S
Sbjct: 61  PETFMNKRRRLYGKVFKSHIFGSPTIVSTDSEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSLLKAQITKDMECYVKLSMENWTETNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKAQITKDMECYV+LSM NWTE N IYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVQLSMANWTENNLIYLQDETKNI 180

Query: 181 AFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVIIPGSTLYRSLQAKRIMVELVKK 240
           +FQ+LVKILISLNPGQRMEFLK+QFQ+FIAGLMALPV IPGS LY+SLQAKR MV+LVKK
Sbjct: 181 SFQILVKILISLNPGQRMEFLKKQFQDFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQEKKGSGISPVPKDVMEVLLKDESGQLTDDLIAGNMIDMMIPGEDSVPILMTLAVKYL 300
           IIQE+K  GISPVPKDVMEVLLKDE+ +LTDDLIA NMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISPVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALRQLTEENLKMKKVKEQLGEPLHWSDYLSSLPFTQSVITETLRMGNIIIGVMRK 360
           SDCPAAL+QLTEENLKMK +KEQLGE LHWSDYL S+PFTQSVITETLRMGNIIIGVMRK
Sbjct: 301 SDCPAALQQLTEENLKMKGLKEQLGETLHWSDYL-SMPFTQSVITETLRMGNIIIGVMRK 360

Query: 361 AMKDMKIKGYRIPKGWSVLTYFRSVHLDDSLYEWPYKFNPWRWQERDLSSCNFTPFGGGQ 420
           AMKDMKIKGY IPKGWSVLTYFRSVHLDD+ Y+ PYKFNPWRWQ+RDLSSCNFTPFGGGQ
Sbjct: 361 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDRDLSSCNFTPFGGGQ 420

Query: 421 RLCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRM 462
           RLCPGLDLARLEASIFLHHFVT FR+    D   + P+VR+
Sbjct: 421 RLCPGLDLARLEASIFLHHFVTHFRFQLLTDEAFH-PSVRL 459

BLAST of Sed0009225 vs. NCBI nr
Match: XP_011659645.1 (LOW QUALITY PROTEIN: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis sativus])

HSP 1 Score: 803.9 bits (2075), Expect = 7.4e-229
Identity = 405/467 (86.72%), Postives = 429/467 (91.86%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFLTTIILYRNCFRLFRSKFCNQLPLGSLGWPFIGETIEFIACAYSDR 60
           MDSLWDV+VVT IF TTIILYRNCFRL RSKFCNQLPLGSLGWPFIGETIEFI+ AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQCDAKAFIPSYPKSLTKLMGRS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQ DAKAF+PSYPKSLT+LMG+S
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSLLKAQITKDMECYVKLSMENWTETNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKAQITKDMECYVKLSM NWTE NPIYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 181 AFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVIIPGSTLYRSLQAKRIMVELVKK 240
           +FQVLVKILISLNPGQ MEFLK+QFQEFIAGLMALPV IPGS LY+SLQAKR MV+LVKK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQEKKGSGISPVPKDVMEVLLKDESGQLTDDLIAGNMIDMMIPGEDSVPILMTLAVKYL 300
           IIQE+K  GIS VPKD+MEVLLKDE+ +LTDDLIA NMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALRQLTEENLKMKKVKEQLGEPLHWSDYLSSLPFTQSVITETLRMGNIIIGVMRK 360
           SDCPAAL+QLTEENLKMK  KEQLGE LHWSDYL S+PFTQSVITETLRMGNIIIGVMRK
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYL-SMPFTQSVITETLRMGNIIIGVMRK 360

Query: 361 AMKDMKIKGYRIPKGWSVLTYFRSVHLDDSLYEWPYKFNPWRWQERDLSSCNFTPFGGGQ 420
           AMKDMKIKGY IPKGWSVLTYFRSVHLDD+ Y+ PYKFNPWRWQ+R++SSCNFTPFGGGQ
Sbjct: 361 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQ 420

Query: 421 RLCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPV 468
           RLCPGLDLARLEASIFLHHFVT FR+    D   + P+VR     P+
Sbjct: 421 RLCPGLDLARLEASIFLHHFVTHFRFQLLTDEAFH-PSVRFTXLPPL 465

BLAST of Sed0009225 vs. NCBI nr
Match: KAE8646515.1 (hypothetical protein Csa_016429 [Cucumis sativus])

HSP 1 Score: 801.6 bits (2069), Expect = 3.7e-228
Identity = 400/445 (89.89%), Postives = 420/445 (94.38%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFLTTIILYRNCFRLFRSKFCNQLPLGSLGWPFIGETIEFIACAYSDR 60
           MDSLWDV+VVT IF TTIILYRNCFRL RSKFCNQLPLGSLGWPFIGETIEFI+ AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQCDAKAFIPSYPKSLTKLMGRS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQ DAKAF+PSYPKSLT+LMG+S
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSLLKAQITKDMECYVKLSMENWTETNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKAQITKDMECYVKLSM NWTE NPIYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 181 AFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVIIPGSTLYRSLQAKRIMVELVKK 240
           +FQVLVKILISLNPGQ MEFLK+QFQEFIAGLMALPV IPGS LY+SLQAKR MV+LVKK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQEKKGSGISPVPKDVMEVLLKDESGQLTDDLIAGNMIDMMIPGEDSVPILMTLAVKYL 300
           IIQE+K  GIS VPKD+MEVLLKDE+ +LTDDLIA NMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALRQLTEENLKMKKVKEQLGEPLHWSDYLSSLPFTQSVITETLRMGNIIIGVMRK 360
           SDCPAAL+QLTEENLKMK  KEQLGE LHWSDYL S+PFTQSVITETLRMGNIIIGVMRK
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYL-SMPFTQSVITETLRMGNIIIGVMRK 360

Query: 361 AMKDMKIKGYRIPKGWSVLTYFRSVHLDDSLYEWPYKFNPWRWQERDLSSCNFTPFGGGQ 420
           AMKDMKIKGY IPKGWSVLTYFRSVHLDD+ Y+ PYKFNPWRWQ+R++SSCNFTPFGGGQ
Sbjct: 361 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQ 420

Query: 421 RLCPGLDLARLEASIFLHHFVTQFR 446
           RLCPGLDLARLEASIFLHHFVT FR
Sbjct: 421 RLCPGLDLARLEASIFLHHFVTHFR 444

BLAST of Sed0009225 vs. ExPASy Swiss-Prot
Match: Q94IA6 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=3702 GN=CYP90D1 PE=1 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 3.9e-172
Identity = 293/443 (66.14%), Postives = 362/443 (81.72%), Query Frame = 0

Query: 35  QLPLGSLGWPFIGETIEFIACAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVS 94
           + P GSLGWP IGETIEF++ AYSDRPE+FMDKRR +YG+VFKSHIFG+ TIVSTDAEV+
Sbjct: 50  KFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVN 109

Query: 95  KFILQCDAKAFIPSYPKSLTKLMGRSSILLINGSLQKRIHGLVGAFFKSSLLKAQITKDM 154
           + +LQ D+ AF+P YPK++ +LMG+SSILLINGSL +R HGLVG+F KS LLKAQI +DM
Sbjct: 110 RAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDM 169

Query: 155 ECYVKLSMENWTETNPIYLQDETKNIAFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMA 214
             ++  SM+ W+E  P+ LQD +K +AF+VL K LIS+  G+ +E LK++F+ FI+GLM+
Sbjct: 170 HKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMS 229

Query: 215 LPVIIPGSTLYRSLQAKRIMVELVKKIIQ-----EKKGSGISPVPKDVMEVLLKDESGQL 274
           LP+  PG+ L+RSLQAK+ MV+ V++II+      K       + KDV++VLLKD S  L
Sbjct: 230 LPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEHL 289

Query: 275 TDDLIAGNMIDMMIPGEDSVPILMTLAVKYLSDCPAALRQLTEENLKMKKVKEQLGEPLH 334
           T +LIA NMIDMMIPG DSVP+L+TLAVK+LSD PAAL  LTEEN+K+K +KE  GEPL+
Sbjct: 290 THNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLY 349

Query: 335 WSDYLSSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYRIPKGWSVLTYFRSVHLDD 394
           W+DYL SLPFTQ VITETLRMGN+IIGVMRKAMKD++IKGY IPKGW  L Y RSVHLD 
Sbjct: 350 WNDYL-SLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDK 409

Query: 395 SLYEWPYKFNPWRWQERDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIAE 454
             YE PYKFNPWRWQERD+++ +F+PFGGGQRLCPGLDLARLE S+FLHH VT+FRWIAE
Sbjct: 410 LYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLVTRFRWIAE 469

Query: 455 EDRIINFPTVRMKKRMPVWVKRI 473
           ED IINFPTV MK ++P+W+KRI
Sbjct: 470 EDTIINFPTVHMKNKLPIWIKRI 491

BLAST of Sed0009225 vs. ExPASy Swiss-Prot
Match: Q94IW5 (Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 6.0e-165
Identity = 275/443 (62.08%), Postives = 367/443 (82.84%), Query Frame = 0

Query: 35  QLPLGSLGWPFIGETIEFIACAYSDRPETFMDKRRRLYGK-VFKSHIFGSPTIVSTDAEV 94
           +LP GS GWP +GET+EF++CAYS RPE F+DKRR+L+G  VF+SH+FGS T+V+ DAEV
Sbjct: 48  RLPPGSFGWPVVGETLEFVSCAYSPRPEAFVDKRRKLHGSAVFRSHLFGSATVVTADAEV 107

Query: 95  SKFILQCDAKAFIPSYPKSLTKLMGRSSILLINGSLQKRIHGLVGAFFKSSLLKAQITKD 154
           S+F+LQ DA+AF+P YP+SLT+LMG+SSILLING+LQ+R+HGLVGAFFKSS LK+Q+T D
Sbjct: 108 SRFVLQSDARAFVPWYPRSLTELMGKSSILLINGALQRRVHGLVGAFFKSSHLKSQLTAD 167

Query: 155 MECYVKLSMENWTETNPIYLQDETKNIAFQVLVKILISLNPGQRMEFLKQQFQEFIAGLM 214
           M   +  ++ ++ +++ +++Q   K++ F++LV+ LI L  G+ M+ LKQQFQEFI GLM
Sbjct: 168 MRRRLSPALSSFPDSSLLHVQHLAKSVVFEILVRGLIGLEAGEEMQQLKQQFQEFIVGLM 227

Query: 215 ALPVIIPGSTLYRSLQAKRIMVELVKKIIQEKKG--SGISPVPKDVMEVLLKDESGQLTD 274
           +LP+ +PG+ LYRSLQAK+ M  L+++II+EK+   +  SP P+D ++VL+ D S +LTD
Sbjct: 228 SLPIKLPGTRLYRSLQAKKKMARLIQRIIREKRARRAAASP-PRDAIDVLIGDGSDELTD 287

Query: 275 DLIAGNMIDMMIPGEDSVPILMTLAVKYLSDCPAALRQLTEENLKMKKVKEQLGEPLHWS 334
           +LI+ NMID+MIP EDSVP+L+TLAVK+LS+CP AL QL EEN+++K+ K  +GE L W+
Sbjct: 288 ELISDNMIDLMIPAEDSVPVLITLAVKFLSECPLALHQLEEENIQLKRRKTDMGETLQWT 347

Query: 335 DYLSSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYRIPKGWSVLTYFRSVHLDDSL 394
           DY+ SL FTQ VITETLR+GNII G+MRKA++D+++KG+ IPKGW V  YFRSVHLDD+L
Sbjct: 348 DYM-SLSFTQHVITETLRLGNIIGGIMRKAVRDVEVKGHLIPKGWCVFVYFRSVHLDDTL 407

Query: 395 YEWPYKFNPWRWQERDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIAEED 454
           Y+ PYKFNPWRW+E+D+S+ +FTPFGGGQRLCPGLDLARLEASIFLHH VT FRW+AEED
Sbjct: 408 YDEPYKFNPWRWKEKDMSNGSFTPFGGGQRLCPGLDLARLEASIFLHHLVTSFRWVAEED 467

Query: 455 RIINFPTVRMKKRMPVWVKRIRD 475
            I+NFPTVR+K+ MP+ V    D
Sbjct: 468 HIVNFPTVRLKRGMPIRVTAKED 488

BLAST of Sed0009225 vs. ExPASy Swiss-Prot
Match: Q9M066 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=3702 GN=CYP90C1 PE=1 SV=3)

HSP 1 Score: 508.4 bits (1308), Expect = 8.5e-143
Identity = 243/447 (54.36%), Postives = 339/447 (75.84%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFIACAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           +P GSLGWP IGET+ FIAC YS RP TFMDKR+ LYGKVFK++I G+P I+STDAEV+K
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 96  FILQCDAKAFIPSYPKSLTKLMGRSSILLINGSLQKRIHGLVGAFFKSSLLKAQITKDME 155
            +LQ     F+P+YPKS+T+L+G +SIL ING  QKR+H L+GAF +S  LK +IT+D+E
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 156 CYVKLSMENWTETNPIYLQDETKNIAFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMAL 215
             V L++ +W +   +++QDE K + F++LVK+L+S +PG+ M  LK +F+EFI GL+ +
Sbjct: 189 ASVVLTLASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLICI 248

Query: 216 PVIIPGSTLYRSLQAKRIMVELVKKIIQEKKGSGISPVP-KDVMEVLLKD----ESGQLT 275
           P+  PG+ LY+SL+AK  ++++VKK+++E++ +  +  P  DV++VLL+D    E     
Sbjct: 249 PIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDSEKQSQP 308

Query: 276 DDLIAGNMIDMMIPGEDSVPILMTLAVKYLSDCPAALRQLTEENLKMKKVKEQLGEPLHW 335
            D ++G +++MMIPGE+++P  MTLAVK+LSD P AL +L EEN++MK+ K +LGE   W
Sbjct: 309 SDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYKW 368

Query: 336 SDYLSSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYRIPKGWSVLTYFRSVHLDDS 395
           +DY+ SL FTQ+VI ETLRM NII GV RKA+KD++IKGY IPKGW VL  F SVH+D+ 
Sbjct: 369 TDYM-SLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDED 428

Query: 396 LYEWPYKFNPWRWQERD---LSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWI 455
           +Y+ PY+F+PWRW   +    SS  FTPFGGGQRLCPGL+L++LE SIFLHH VT++ W 
Sbjct: 429 IYDNPYQFDPWRWDRINGSANSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRYSWT 488

Query: 456 AEEDRIINFPTVRMKKRMPVWVKRIRD 475
           AEED I++FPTV+MK+R+P+ V  + D
Sbjct: 489 AEEDEIVSFPTVKMKRRLPIRVATVDD 514

BLAST of Sed0009225 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.8e-100
Identity = 190/443 (42.89%), Postives = 292/443 (65.91%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFIACAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           LP GSLG P IGET + I    ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 96  FILQCDAKAFIPSYPKSLTKLMGRSSILLINGSLQKRIHGLVGAFFKSSLLKAQITKDME 155
           F+LQ + K F  SYP S+  L+G+ S+LL+ GSL KR+H L  +F  SS++K  +  D++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 156 CYVKLSMENWTETNPIYLQDETKNIAFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMAL 215
             V+ ++++W  ++ + L +E K I F++ VK L+S +PG+  E L++++   I G  +L
Sbjct: 151 RLVRFNLDSW--SSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSL 210

Query: 216 PVIIPGSTLYRSLQAKRIMVELVKKII---QEKKGSGISPVPKDVMEVLLKDESGQLTDD 275
           P+ +  +T  +++QA+R + E +  ++   +E++  G +   KD++  LL  + G  +D+
Sbjct: 211 PLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEG-AERKKDMLAALLAADDG-FSDE 270

Query: 276 LIAGNMIDMMIPGEDSVPILMTLAVKYLSDCPAALRQLTEENLKMKKVKEQLGEPLHWSD 335
            I   ++ +++ G ++   +MTLAVK+L++ P AL QL EE+ K++ +K      L WSD
Sbjct: 271 EIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSD-SYSLEWSD 330

Query: 336 YLSSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYRIPKGWSVLTYFRSVHLDDSLY 395
           Y  S+PFTQ V+ ETLR+ NII GV R+AM D++IKGY+IPKGW V + FR+VHLD + +
Sbjct: 331 Y-KSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHF 390

Query: 396 EWPYKFNPWRWQERDLS---SCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWI-A 455
           +    FNPWRWQ   ++   S  FTPFGGG RLCPG +LAR+  S+FLH  VT F W+ A
Sbjct: 391 KDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHRLVTGFSWVPA 450

Query: 456 EEDRIINFPTVRMKKRMPVWVKR 472
           E+D+++ FPT R +KR P++VKR
Sbjct: 451 EQDKLVFFPTTRTQKRYPIFVKR 467

BLAST of Sed0009225 vs. ExPASy Swiss-Prot
Match: Q5CCK1 (Cytochrome P450 90A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90A4 PE=2 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 7.3e-86
Identity = 180/457 (39.39%), Postives = 268/457 (58.64%), Query Frame = 0

Query: 35  QLPLGSLGWPFIGETIEFIACAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVS 94
           ++P GS G P IGET+  I+   +  PE F+D+R   +G VF +H+FG  T+ S D   +
Sbjct: 41  RMPPGSTGLPLIGETLRLISAYKTPNPEPFIDERVARHGGVFTTHVFGERTVFSADPAFN 100

Query: 95  KFILQCDAKAFIPSYPKSLTKLMGRSSILLINGSLQKRIHGLVGAFF---KSSLLKAQIT 154
           + +L  + +A   SYP S+  L+G  S+LL  G+  KR+H L         S  L A I 
Sbjct: 101 RLLLAAEGRAVHSSYPSSIATLLGARSLLLTRGAAHKRLHSLTLTRLGRPASPPLLAHID 160

Query: 155 KDMECYVKLSMENWTETNPIYLQDETKNIAFQVLVKILISLNPGQRMEFLKQQFQEFIAG 214
           +     V  +M  W     + L DE K I F + VK L+S+ PG   E L++++ + I G
Sbjct: 161 R----LVLATMRQWEPAATVRLMDEAKKITFNLTVKQLVSIEPGPWTESLRREYVKLIDG 220

Query: 215 LMALP----VIIPGSTLYRSLQAKR----IMVELVKKIIQEKKGSGIS-------PVPKD 274
             ++P      +P +T  ++L+A++     + E++KK ++EK  +G S          KD
Sbjct: 221 FFSIPFPLAYFLPFTTYGQALKARKKVAGALREVIKKRMEEKAENGGSIGDDEGKKEKKD 280

Query: 275 VMEVLLKDESGQLTDDLIAGNMIDMMIPGEDSVPILMTLAVKYLSDCPAALRQLTEENLK 334
           ++E LL+ E G  +++ +    + +++ G ++  +LMTLAVK+L++ PAAL +L EE+  
Sbjct: 281 MVEELLQAEGGSFSEEEMVDFCLSLLVAGYETTSVLMTLAVKFLTETPAALAELKEEHAN 340

Query: 335 MKKVKEQLGEPLHWSDYLSSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYRIPKGW 394
           ++ +K +  +PL WSDY  S+PFTQ VI ETLR+GNII GV R+A  D+  K Y IPKG 
Sbjct: 341 IRDMKGK-NQPLEWSDY-KSMPFTQCVINETLRVGNIISGVFRRANTDIHYKDYTIPKGC 400

Query: 395 SVLTYFRSVHLDDSLYEWPYKFNPWRWQ-----ERDLSSCNFTPFGGGQRLCPGLDLARL 454
            +   FR+VHL++  YE    FNPWRWQ     +  + +  FTPFGGG RLCPG +LAR+
Sbjct: 401 KIFASFRAVHLNNEHYENARTFNPWRWQINNKLQNAVGANIFTPFGGGPRLCPGYELARV 460

Query: 455 EASIFLHHFVTQFRW-IAEEDRIINFPTVRMKKRMPV 468
             SIFLHH VT+F W   EEDR++ FPT R  K  P+
Sbjct: 461 VVSIFLHHLVTRFSWEETEEDRLVFFPTTRTLKGYPI 491

BLAST of Sed0009225 vs. ExPASy TrEMBL
Match: A0A0A0K7W8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G451390 PE=3 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.5e-246
Identity = 428/475 (90.11%), Postives = 449/475 (94.53%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFLTTIILYRNCFRLFRSKFCNQLPLGSLGWPFIGETIEFIACAYSDR 60
           MDSLWDV+VVT IF TTIILYRNCFRL RSKFCNQLPLGSLGWPFIGETIEFI+ AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQCDAKAFIPSYPKSLTKLMGRS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQ DAKAF+PSYPKSLT+LMG+S
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSLLKAQITKDMECYVKLSMENWTETNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKAQITKDMECYVKLSM NWTE NPIYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 181 AFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVIIPGSTLYRSLQAKRIMVELVKK 240
           +FQVLVKILISLNPGQ MEFLK+QFQEFIAGLMALPV IPGS LY+SLQAKR MV+LVKK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQEKKGSGISPVPKDVMEVLLKDESGQLTDDLIAGNMIDMMIPGEDSVPILMTLAVKYL 300
           IIQE+K  GIS VPKD+MEVLLKDE+ +LTDDLIA NMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALRQLTEENLKMKKVKEQLGEPLHWSDYLSSLPFTQSVITETLRMGNIIIGVMRK 360
           SDCPAAL+QLTEENLKMK  KEQLGE LHWSDYL S+PFTQSVITETLRMGNIIIGVMRK
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYL-SMPFTQSVITETLRMGNIIIGVMRK 360

Query: 361 AMKDMKIKGYRIPKGWSVLTYFRSVHLDDSLYEWPYKFNPWRWQERDLSSCNFTPFGGGQ 420
           AMKDMKIKGY IPKGWSVLTYFRSVHLDD+ Y+ PYKFNPWRWQ+R++SSCNFTPFGGGQ
Sbjct: 361 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQ 420

Query: 421 RLCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPVWVKRIRDP 476
           RLCPGLDLARLEASIFLHHFVT FRWIAEED+IINFPTVRMKKRMPVWVKR RDP
Sbjct: 421 RLCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP 474

BLAST of Sed0009225 vs. ExPASy TrEMBL
Match: A0A1S3CGU6 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103500734 PE=3 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 1.2e-243
Identity = 428/475 (90.11%), Postives = 447/475 (94.11%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFLTTIILYRNCFRLFRSKFCNQLPLGSLGWPFIGETIEFIACAYSDR 60
           MDSLWDV+VVT I   TIILYRNCFRL RSKFCNQLPLGSLGWPFIGETIEFI+CAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQCDAKAFIPSYPKSLTKLMGRS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQ DAKAF+PSYPKSLT+LMG+S
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSLLKAQITKDMECYVKLSMENWTETNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKAQITKDMECYVKLSM +WTE NPIYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 AFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVIIPGSTLYRSLQAKRIMVELVKK 240
           +FQVLVKILISLNPGQ MEFLK+QFQEFIAGLMALPV IPGS LY+SLQAKR MV+LVKK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQEKKGSGISPVPKDVMEVLLKDESGQLTDDLIAGNMIDMMIPGEDSVPILMTLAVKYL 300
           IIQE+K  GIS VPKDVMEVLLKDE+ +LTDDLIA NMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALRQLTEENLKMKKVKEQLGEPLHWSDYLSSLPFTQSVITETLRMGNIIIGVMRK 360
           SDCPAAL+QLTEENLKMK  KEQLGE LHWSDYL S+PFTQSVITETLRMGNIIIGVMRK
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYL-SMPFTQSVITETLRMGNIIIGVMRK 360

Query: 361 AMKDMKIKGYRIPKGWSVLTYFRSVHLDDSLYEWPYKFNPWRWQERDLSSCNFTPFGGGQ 420
           AMKDMKIKGY I KGWSVLTYFRSVHLDD+ Y+ PYKFNPWRWQER+LSSCNFTPFGGGQ
Sbjct: 361 AMKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQ 420

Query: 421 RLCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPVWVKRIRDP 476
           RLCPGLDLARLEASIFLHHFVT FRWIAEED+IINFPTVRMKKRMPVWVKR RDP
Sbjct: 421 RLCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP 471

BLAST of Sed0009225 vs. ExPASy TrEMBL
Match: E5GCA5 (Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 1.7e-226
Identity = 405/467 (86.72%), Postives = 427/467 (91.43%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFLTTIILYRNCFRLFRSKFCNQLPLGSLGWPFIGETIEFIACAYSDR 60
           MDSLWDV+VVT I   TIILYRNCFRL RSKFCNQLPLGSLGWPFIGETIEFI+CAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQCDAKAFIPSYPKSLTKLMGRS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQ DAKAF+PSYPKSLT+LMG+S
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSLLKAQITKDMECYVKLSMENWTETNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKAQITKDMECYVKLSM +WTE NPIYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 AFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVIIPGSTLYRSLQAKRIMVELVKK 240
           +FQVLVKILISLNPGQ MEFLK+QFQEFIAGLMALPV IPGS LY+SLQAKR MV+LVKK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQEKKGSGISPVPKDVMEVLLKDESGQLTDDLIAGNMIDMMIPGEDSVPILMTLAVKYL 300
           IIQE+K  GIS VPKDVMEVLLKDE+ +LTDDLIA NMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALRQLTEENLKMKKVKEQLGEPLHWSDYLSSLPFTQSVITETLRMGNIIIGVMRK 360
           SDCPAAL+QLTEENLKMK  KEQLGE LHWSDYL S+PFTQSVITETLRMGNIIIGVMRK
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYL-SMPFTQSVITETLRMGNIIIGVMRK 360

Query: 361 AMKDMKIKGYRIPKGWSVLTYFRSVHLDDSLYEWPYKFNPWRWQERDLSSCNFTPFGGGQ 420
           AMKDMKIKGY I KGWSVLTYFRSVHLDD+ Y+ PYKFNPWRWQER+LSSCNFTPFGGGQ
Sbjct: 361 AMKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQ 420

Query: 421 RLCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPV 468
           RLCPGLDLARLEASIFLHHFVT FR+    D   + P+VR     P+
Sbjct: 421 RLCPGLDLARLEASIFLHHFVTHFRFQLLTDEAFH-PSVRFTNFPPL 462

BLAST of Sed0009225 vs. ExPASy TrEMBL
Match: A0A5A7UW93 (Cytochrome p450 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G001660 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 6.4e-226
Identity = 406/471 (86.20%), Postives = 428/471 (90.87%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFLTTIILYRNCFRLFRSKFCNQLPLGSLGWPFIGETIEFIACAYSDR 60
           MDSLWDV+VVT I   TIILYRNCFRL RSKFCNQLPLGSLGWPFIGETIEFI+CAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQCDAKAFIPSYPKSLTKLMGRS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQ DAKAF+PSYPKSLT+LMG+S
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSLLKAQITKDMECYVKLSMENWTETNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKAQITKDMECYVKLSM +WTE NPIYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 AFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVIIPGSTLYRSLQ----AKRIMVE 240
           +FQVLVKILISLNPGQ MEFLK+QFQEFIAGLMALPV IPGS LY+SLQ    AKR MV+
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQASWTAKRNMVK 240

Query: 241 LVKKIIQEKKGSGISPVPKDVMEVLLKDESGQLTDDLIAGNMIDMMIPGEDSVPILMTLA 300
           LVKKIIQE+K  GIS VPKDVMEVLLKDE+ +LTDDLIA NMIDMMIPGEDSVPILMTLA
Sbjct: 241 LVKKIIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLA 300

Query: 301 VKYLSDCPAALRQLTEENLKMKKVKEQLGEPLHWSDYLSSLPFTQSVITETLRMGNIIIG 360
           VKYLSDCPAAL+QLTEENLKMK  KEQLGE LHWSDYL S+PFTQSVITETLRMGNIIIG
Sbjct: 301 VKYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYL-SMPFTQSVITETLRMGNIIIG 360

Query: 361 VMRKAMKDMKIKGYRIPKGWSVLTYFRSVHLDDSLYEWPYKFNPWRWQERDLSSCNFTPF 420
           VMRKAMKDMKIKGY IPKGWSVLTYFRSVHLDD+ Y+ PYKFNPWRWQER+LSSCNFTPF
Sbjct: 361 VMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPF 420

Query: 421 GGGQRLCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPV 468
           GGGQRLCPGLDLARLEASIFLHHFVT FR+    D   + P+VR     P+
Sbjct: 421 GGGQRLCPGLDLARLEASIFLHHFVTHFRFQLLTDEAFH-PSVRFTNFPPL 466

BLAST of Sed0009225 vs. ExPASy TrEMBL
Match: A0A6P3YVG2 (3-epi-6-deoxocathasterone 23-monooxygenase isoform X1 OS=Ziziphus jujuba OX=326968 GN=LOC107403831 PE=3 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 4.3e-214
Identity = 373/479 (77.87%), Postives = 428/479 (89.35%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFLTTII--LYRNCFRLFRSKFCNQ-LPLGSLGWPFIGETIEFIACAY 60
           MD LW ++ VT +FL+ II  LY+N +R FRSK   + LPLG+LGWPF+GETIEF++CAY
Sbjct: 1   MDKLW-ILQVTAVFLSAIILLLYKNSYR-FRSKHRRKPLPLGTLGWPFLGETIEFVSCAY 60

Query: 61  SDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQCDAKAFIPSYPKSLTKLM 120
           SDRPE+FMDKRR +YGKVFKSHIFGSPTIVSTDAEVSKF+LQ DAKAF+PSYPKSLT+LM
Sbjct: 61  SDRPESFMDKRRSMYGKVFKSHIFGSPTIVSTDAEVSKFVLQSDAKAFVPSYPKSLTELM 120

Query: 121 GRSSILLINGSLQKRIHGLVGAFFKSSLLKAQITKDMECYVKLSMENWTETNPIYLQDET 180
           G+SSILLINGSLQ+RIHGLVGAFFKS  LKAQIT+DM+ YV+ SM NW+E +P Y+QD T
Sbjct: 121 GKSSILLINGSLQRRIHGLVGAFFKSPHLKAQITRDMQKYVQESMANWSEDHPTYIQDVT 180

Query: 181 KNIAFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVIIPGSTLYRSLQAKRIMVEL 240
           K IAFQVLVK LISLNPG  MEFLK+QFQEFIAGLM+LP+ IPGSTLYRSLQAK+ MV+L
Sbjct: 181 KTIAFQVLVKALISLNPGDEMEFLKKQFQEFIAGLMSLPINIPGSTLYRSLQAKKKMVKL 240

Query: 241 VKKIIQEKKG-SGISPVPKDVMEVLLKDESGQLTDDLIAGNMIDMMIPGEDSVPILMTLA 300
           VKK IQ +K  SGIS  PKDV++VLL D S QLTDDLIA NMIDMMIPGEDSVP+LMTLA
Sbjct: 241 VKKTIQARKNTSGISNFPKDVVDVLLSDTSEQLTDDLIADNMIDMMIPGEDSVPVLMTLA 300

Query: 301 VKYLSDCPAALRQLTEENLKMKKVKEQLGEPLHWSDYLSSLPFTQSVITETLRMGNIIIG 360
           +KYLSDCPAAL+QL EEN+K+K+ K+QLGEPL WSDYL SLPFTQ+VITETLRMGNIIIG
Sbjct: 301 IKYLSDCPAALQQLMEENMKLKRHKDQLGEPLCWSDYL-SLPFTQNVITETLRMGNIIIG 360

Query: 361 VMRKAMKDMKIKGYRIPKGWSVLTYFRSVHLDDSLYEWPYKFNPWRWQERDLSSCNFTPF 420
           VMRKAMKD++IKGY IPKGW V  YFRSVHLD++ Y+WPY+FNPWRWQ++D+S+CNFTPF
Sbjct: 361 VMRKAMKDVEIKGYLIPKGWCVFAYFRSVHLDENHYDWPYEFNPWRWQDKDMSTCNFTPF 420

Query: 421 GGGQRLCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPVWVKRIRDP 476
           GGGQRLCPGLDLARLEASIFLHHFVTQFRW+AE+D I+NFPTVRMK+RMP+WVKR  DP
Sbjct: 421 GGGQRLCPGLDLARLEASIFLHHFVTQFRWVAEDDTIVNFPTVRMKRRMPIWVKRRGDP 476

BLAST of Sed0009225 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 605.9 bits (1561), Expect = 2.8e-173
Identity = 293/443 (66.14%), Postives = 362/443 (81.72%), Query Frame = 0

Query: 35  QLPLGSLGWPFIGETIEFIACAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVS 94
           + P GSLGWP IGETIEF++ AYSDRPE+FMDKRR +YG+VFKSHIFG+ TIVSTDAEV+
Sbjct: 50  KFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVN 109

Query: 95  KFILQCDAKAFIPSYPKSLTKLMGRSSILLINGSLQKRIHGLVGAFFKSSLLKAQITKDM 154
           + +LQ D+ AF+P YPK++ +LMG+SSILLINGSL +R HGLVG+F KS LLKAQI +DM
Sbjct: 110 RAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDM 169

Query: 155 ECYVKLSMENWTETNPIYLQDETKNIAFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMA 214
             ++  SM+ W+E  P+ LQD +K +AF+VL K LIS+  G+ +E LK++F+ FI+GLM+
Sbjct: 170 HKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMS 229

Query: 215 LPVIIPGSTLYRSLQAKRIMVELVKKIIQ-----EKKGSGISPVPKDVMEVLLKDESGQL 274
           LP+  PG+ L+RSLQAK+ MV+ V++II+      K       + KDV++VLLKD S  L
Sbjct: 230 LPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEHL 289

Query: 275 TDDLIAGNMIDMMIPGEDSVPILMTLAVKYLSDCPAALRQLTEENLKMKKVKEQLGEPLH 334
           T +LIA NMIDMMIPG DSVP+L+TLAVK+LSD PAAL  LTEEN+K+K +KE  GEPL+
Sbjct: 290 THNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLY 349

Query: 335 WSDYLSSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYRIPKGWSVLTYFRSVHLDD 394
           W+DYL SLPFTQ VITETLRMGN+IIGVMRKAMKD++IKGY IPKGW  L Y RSVHLD 
Sbjct: 350 WNDYL-SLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDK 409

Query: 395 SLYEWPYKFNPWRWQERDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIAE 454
             YE PYKFNPWRWQERD+++ +F+PFGGGQRLCPGLDLARLE S+FLHH VT+FRWIAE
Sbjct: 410 LYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLVTRFRWIAE 469

Query: 455 EDRIINFPTVRMKKRMPVWVKRI 473
           ED IINFPTV MK ++P+W+KRI
Sbjct: 470 EDTIINFPTVHMKNKLPIWIKRI 491

BLAST of Sed0009225 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 508.4 bits (1308), Expect = 6.0e-144
Identity = 243/447 (54.36%), Postives = 339/447 (75.84%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFIACAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           +P GSLGWP IGET+ FIAC YS RP TFMDKR+ LYGKVFK++I G+P I+STDAEV+K
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 96  FILQCDAKAFIPSYPKSLTKLMGRSSILLINGSLQKRIHGLVGAFFKSSLLKAQITKDME 155
            +LQ     F+P+YPKS+T+L+G +SIL ING  QKR+H L+GAF +S  LK +IT+D+E
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 156 CYVKLSMENWTETNPIYLQDETKNIAFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMAL 215
             V L++ +W +   +++QDE K + F++LVK+L+S +PG+ M  LK +F+EFI GL+ +
Sbjct: 189 ASVVLTLASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLICI 248

Query: 216 PVIIPGSTLYRSLQAKRIMVELVKKIIQEKKGSGISPVP-KDVMEVLLKD----ESGQLT 275
           P+  PG+ LY+SL+AK  ++++VKK+++E++ +  +  P  DV++VLL+D    E     
Sbjct: 249 PIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDSEKQSQP 308

Query: 276 DDLIAGNMIDMMIPGEDSVPILMTLAVKYLSDCPAALRQLTEENLKMKKVKEQLGEPLHW 335
            D ++G +++MMIPGE+++P  MTLAVK+LSD P AL +L EEN++MK+ K +LGE   W
Sbjct: 309 SDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYKW 368

Query: 336 SDYLSSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYRIPKGWSVLTYFRSVHLDDS 395
           +DY+ SL FTQ+VI ETLRM NII GV RKA+KD++IKGY IPKGW VL  F SVH+D+ 
Sbjct: 369 TDYM-SLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDED 428

Query: 396 LYEWPYKFNPWRWQERD---LSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWI 455
           +Y+ PY+F+PWRW   +    SS  FTPFGGGQRLCPGL+L++LE SIFLHH VT++ W 
Sbjct: 429 IYDNPYQFDPWRWDRINGSANSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRYSWT 488

Query: 456 AEEDRIINFPTVRMKKRMPVWVKRIRD 475
           AEED I++FPTV+MK+R+P+ V  + D
Sbjct: 489 AEEDEIVSFPTVKMKRRLPIRVATVDD 514

BLAST of Sed0009225 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 367.9 bits (943), Expect = 1.3e-101
Identity = 190/443 (42.89%), Postives = 292/443 (65.91%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFIACAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           LP GSLG P IGET + I    ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 96  FILQCDAKAFIPSYPKSLTKLMGRSSILLINGSLQKRIHGLVGAFFKSSLLKAQITKDME 155
           F+LQ + K F  SYP S+  L+G+ S+LL+ GSL KR+H L  +F  SS++K  +  D++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 156 CYVKLSMENWTETNPIYLQDETKNIAFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMAL 215
             V+ ++++W  ++ + L +E K I F++ VK L+S +PG+  E L++++   I G  +L
Sbjct: 151 RLVRFNLDSW--SSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSL 210

Query: 216 PVIIPGSTLYRSLQAKRIMVELVKKII---QEKKGSGISPVPKDVMEVLLKDESGQLTDD 275
           P+ +  +T  +++QA+R + E +  ++   +E++  G +   KD++  LL  + G  +D+
Sbjct: 211 PLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEG-AERKKDMLAALLAADDG-FSDE 270

Query: 276 LIAGNMIDMMIPGEDSVPILMTLAVKYLSDCPAALRQLTEENLKMKKVKEQLGEPLHWSD 335
            I   ++ +++ G ++   +MTLAVK+L++ P AL QL EE+ K++ +K      L WSD
Sbjct: 271 EIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSD-SYSLEWSD 330

Query: 336 YLSSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYRIPKGWSVLTYFRSVHLDDSLY 395
           Y  S+PFTQ V+ ETLR+ NII GV R+AM D++IKGY+IPKGW V + FR+VHLD + +
Sbjct: 331 Y-KSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHF 390

Query: 396 EWPYKFNPWRWQERDLS---SCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWI-A 455
           +    FNPWRWQ   ++   S  FTPFGGG RLCPG +LAR+  S+FLH  VT F W+ A
Sbjct: 391 KDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHRLVTGFSWVPA 450

Query: 456 EEDRIINFPTVRMKKRMPVWVKR 472
           E+D+++ FPT R +KR P++VKR
Sbjct: 451 EQDKLVFFPTTRTQKRYPIFVKR 467

BLAST of Sed0009225 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 313.9 bits (803), Expect = 2.2e-85
Identity = 167/497 (33.60%), Postives = 291/497 (58.55%), Query Frame = 0

Query: 15  LTTIILYRNCFRLFRSKFCNQLPLGSLGWPFIGETIEFIACAYSDRPETFMDKRRRLYGK 74
           L +++L+    +    K    LP G  GWPF+GETI ++    +     FM +    YGK
Sbjct: 18  LLSLLLFLILLKRRNRKTRFNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGK 77

Query: 75  VFKSHIFGSPTIVSTDAEVSKFILQCDAKAFIPSYPKSLTKLMGRSSILLINGSLQKRIH 134
           +++S++FG PTIVS DA +++FILQ + + F  SYP+S+  ++G+ S+L++ G + + + 
Sbjct: 78  IYRSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMR 137

Query: 135 GLVGAFFKSSLLKAQITKDMECYVKLSMENWTETNPIYLQDETKNIAFQVLVKILISLNP 194
            +   F   + L+  + KD+E +    +++W + +    QDE K   F ++ K ++S++P
Sbjct: 138 SISLNFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDP 197

Query: 195 G-QRMEFLKQQFQEFIAGLMALPVIIPGSTLYRSLQAKRIMVELVKKIIQEKKGSGISPV 254
           G +  E LK+++  F+ G+++ P+ +PG+  +++LQ++  +++ +++ ++E+K   I   
Sbjct: 198 GEEETEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERK-LDIKEE 257

Query: 255 PKDVMEVLLKDES---------GQLTDDLIAG---------------NMIDMMIPGEDSV 314
            ++  EV  +DE+          Q TDD + G                ++ ++  G ++ 
Sbjct: 258 DQEEEEVKTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETS 317

Query: 315 PILMTLAVKYLSDCPAALRQLTEENLKMKKVKEQLGE-PLHWSDYLSSLPFTQSVITETL 374
            + + LA+ +L  CP A+ +L EE+L++ + K++LGE  L+W DY   + FTQ VI ETL
Sbjct: 318 SVAIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDY-KKMDFTQCVINETL 377

Query: 375 RMGNIIIGVMRKAMKDMKIKGYRIPKGWSVLTYFRSVHLDDSLYEWPYKFNPWRWQERDL 434
           R+GN++  + RKA+KD++ KGY IP GW VL    +VHLD+S Y+ P  FNPWRWQ+++ 
Sbjct: 378 RLGNVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNN 437

Query: 435 SSC------------NFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRW-IAEEDRIIN 473
            +             N+ PFGGG RLC G +LA+LE ++F+HH V +F W +AE+D+   
Sbjct: 438 GASSSGSGSFSTWGNNYMPFGGGPRLCAGSELAKLEMAVFIHHLVLKFNWELAEDDKPFA 497

BLAST of Sed0009225 vs. TAIR 10
Match: AT5G05690.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 293.5 bits (750), Expect = 3.0e-79
Identity = 153/373 (41.02%), Postives = 243/373 (65.15%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFIACAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           LP GSLG P IGET + I    ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 96  FILQCDAKAFIPSYPKSLTKLMGRSSILLINGSLQKRIHGLVGAFFKSSLLKAQITKDME 155
           F+LQ + K F  SYP S+  L+G+ S+LL+ GSL KR+H L  +F  SS++K  +  D++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 156 CYVKLSMENWTETNPIYLQDETKNIAFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMAL 215
             V+ ++++W  ++ + L +E K I F++ VK L+S +PG+  E L++++   I G  +L
Sbjct: 151 RLVRFNLDSW--SSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSL 210

Query: 216 PVIIPGSTLYRSLQAKRIMVELVKKII---QEKKGSGISPVPKDVMEVLLKDESGQLTDD 275
           P+ +  +T  +++QA+R + E +  ++   +E++  G +   KD++  LL  + G  +D+
Sbjct: 211 PLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEG-AERKKDMLAALLAADDG-FSDE 270

Query: 276 LIAGNMIDMMIPGEDSVPILMTLAVKYLSDCPAALRQLTEENLKMKKVKEQLGEPLHWSD 335
            I   ++ +++ G ++   +MTLAVK+L++ P AL QL EE+ K++ +K      L WSD
Sbjct: 271 EIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSD-SYSLEWSD 330

Query: 336 YLSSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYRIPKGWSVLTYFRSVHLDDSLY 395
           Y  S+PFTQ V+ ETLR+ NII GV R+AM D++IKGY+IPKGW V + FR+VHLD + +
Sbjct: 331 Y-KSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHF 390

Query: 396 EWPYKFNPWRWQE 406
           +    FNPWRWQ+
Sbjct: 391 KDARTFNPWRWQQ 397

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008462361.12.4e-24390.11PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo][more]
KAG6575481.15.9e-23481.583-epi-6-deoxocathasterone 23-monooxygenase CYP90D1, partial [Cucurbita argyrospe... [more]
XP_038898401.16.7e-23087.64LOW QUALITY PROTEIN: cytochrome P450 90D2-like [Benincasa hispida][more]
XP_011659645.17.4e-22986.72LOW QUALITY PROTEIN: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis... [more]
KAE8646515.13.7e-22889.89hypothetical protein Csa_016429 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q94IA63.9e-17266.143-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=37... [more]
Q94IW56.0e-16562.08Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV... [more]
Q9M0668.5e-14354.363-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=37... [more]
Q425691.8e-10042.89Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1[more]
Q5CCK17.3e-8639.39Cytochrome P450 90A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90A4 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A0A0K7W82.5e-24690.11Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G451390 PE=3 SV=1[more]
A0A1S3CGU61.2e-24390.113-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC1035007... [more]
E5GCA51.7e-22686.72Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A5A7UW936.4e-22686.20Cytochrome p450 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G0016... [more]
A0A6P3YVG24.3e-21477.873-epi-6-deoxocathasterone 23-monooxygenase isoform X1 OS=Ziziphus jujuba OX=3269... [more]
Match NameE-valueIdentityDescription
AT3G13730.12.8e-17366.14cytochrome P450, family 90, subfamily D, polypeptide 1 [more]
AT4G36380.16.0e-14454.36Cytochrome P450 superfamily protein [more]
AT5G05690.11.3e-10142.89Cytochrome P450 superfamily protein [more]
AT3G50660.12.2e-8533.60Cytochrome P450 superfamily protein [more]
AT5G05690.23.0e-7941.02Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 423..446
score: 32.35
coord: 341..359
score: 24.05
coord: 413..423
score: 51.88
coord: 69..88
score: 25.43
coord: 381..405
score: 26.91
IPR001128Cytochrome P450PFAMPF00067p450coord: 37..451
e-value: 4.2E-49
score: 167.5
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 30..472
e-value: 2.4E-95
score: 321.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 35..471
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 9..444
e-value: 6.1E-10
score: 36.4
NoneNo IPR availablePANTHERPTHR24286:SF303-EPI-6-DEOXOCATHASTERONE 23-MONOOXYGENASE CYP90D1coord: 14..467
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 14..467
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 416..425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0009225.1Sed0009225.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen