Sed0008313 (gene) Chayote v1

Overview
NameSed0008313
Typegene
OrganismSechium edule (Chayote v1)
Descriptiontranscription factor bHLH146-like
LocationLG03: 45084378 .. 45086582 (+)
RNA-Seq ExpressionSed0008313
SyntenySed0008313
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAATGGCTCAATCAGCAACTGAATTTGCATGGGGAAATGCTCTAAAAAAGAAGCTTCTAATTCAAAGACAAGAAGTTTGCAATAATGGTTTTGATTTTCCTCTCCAAACAGTGAAATTTTTTCAAGATAAAAATTTAGAAAGAGAAGAAGAAGGAGAAGAGAAAAAGATGGAAAATGGATTGATGAAGCTGAGAAAAATCTTACCAGGTGGTAATGGTGATTCTAATGGATATTTGGAAGAAGATGATTTGTTGAAACAAACTGAAAGCTATATAAAATGTCTTGAATTGCAGGTCAATGTTCTTAGATGTTTGGTTGATACAAACTCAATTTGATTTTCTTCTTTTTTTTTCTTTCAATTTTTCTTGTTAATTTCTATGTGGAAAAAGAAAAAGAAGTAATGTATGAATTTGAAGGTATCAAAACATTTGTGTGTGATACATTTGTTTTCTCTTTTCTTACCTTTATTTATTTATCTTTATCCTTTTCAATCTAATTAACTCAAAAGGAGTTGAGTTCGAGTGACATCATATATAACTCGGTATCCAAGAAGTTACAGATTCGAATCTCCATCTCGAATGGTAGTTGAATTCAAACAAACTTAAAATTTGTTTATAAAATGTTCTAAGAGTTATGCTTATACTTATTAACCATTTTTTAGAACAAGGTTATTATTTGGGATAGGTGGATCATTGAATTTTTATTGTACTATGTAGGTTCCACAATTGTTCTCAAGTATGTGAAAACAATTTATTTTACTAATACTATATAAATTTTAGTTTTTATTTCTTACACAAACACACTCAAGTCAAAGAGTGATAGAGACTTGGTCAACACTTTTGATGAAGAATTTTTTTTTCAGCCATTGGTATTTTGCTAAGAAATTTAAGTCTAGCTAGCTAAGATGTAAAGTACTAACTGTCAAGATTTTAATCTAACTCAAAACATATACAATTAAGTCTATTTCAAACCCAATTTCTATTTCAAACCCAATTTTTTTTTATATAATCTCCAACTTAAGAAGAAGTAATTCTAAAAATATGTTTGATTTTTTATTCTTTATTTTTTATTTTTTTATAATTTGACTGAGAGTTTTTATTATAAAAAATTTCATAAAGCAATGAGACTTTAATTAAATGTTTTAGAATTATTTTGTAATTATAAAAATTATATTGTTGGATTCCATTGACTTCTCAACCAAAATCTTGGAAGTTTGAGATGTACAGATATGAAAGAGGTCAACCCCTTTGACAAGTCTATAAAGAATCTATGTAGTTGAATTCTCTTGTCCATGTCACAGAGACTGACCAATCACAGAAAAGTCAAAATAAACAAATAAAATTCAAAAGATTATAGAATTTTCTTTTAATAAGTGAACTGTTTTTTTAAAAAGATAGATACAGTACAGTTTTTTTTAAAGAACATATGGTACACTTTTAAATATATCATCTATTGTGTATAGAACTCATTAAAACTCAATTAGATATTCAATCTTAGAAAATCATATCCCAAAAAGTCATCATGCCGAAGATCTTATGTTGAAAAAGTGAACAAACACCATAACTTTTGTAAGATATATAAGTTACTCTATTCATTATCAATTACATATGTTATCTGTGAACAAATTGAACACAATTTTTTCCTAATTTTAATCTAAATTTGTTCTACTAGTTCAAAGGGTAATGTTTGAATTTTTAAGAAGAATCAGATATGAATTCAATAGTGGAAATCTGTTAATTGTAAGATTATTGTATGCAAATTGTTATTTAAGGTTAATTCTTGTATTATGGGTCATGACTATCTTTGTATTGATGCAAGATTCGTTACTAGATGTTACATACAAGAAGGGATAGATTACATTGAATTTTTGTTTTCTCGAGCTACGTTCTTGCATGGAACCTTATATGAGACATTTATGGCTTTAAGCAGCCATTTAGTTAGTACAATAAATTTTTACTCTTTCTTTATCTGAAAATAGTTTTCCAAGAAATTTCTTTTGGCCCATATGTTTACATAAAATTATCAACGCGAGTTGCTTCATCTTACTTCTGTTGTATCGAGATGATATTATATTCCCATTGGGTAGTGTTGTTGATATTTAAAAAACCAAACGTGTCTTAAATAGTAAATTTGAGATGAAAGATAGATCCTGGTACTACAAAAACAATTCTAGATATGGTGTTGATCAGAGACCATGGTAA

mRNA sequence

ATGGCAATGGCTCAATCAGCAACTGAATTTGCATGGGGAAATGCTCTAAAAAAGAAGCTTCTAATTCAAAGACAAGAAGTTTGCAATAATGGTTTTGATTTTCCTCTCCAAACAGTGAAATTTTTTCAAGATAAAAATTTAGAAAGAGAAGAAGAAGGAGAAGAGAAAAAGATGGAAAATGGATTGATGAAGCTGAGAAAAATCTTACCAGGTGGTAATGGTGATTCTAATGGATATTTGGAAGAAGATGATTTGTTGAAACAAACTGAAAGCTATATAAAATGTCTTGAATTGCAGATGAAAGATAGATCCTGGTACTACAAAAACAATTCTAGATATGGTGTTGATCAGAGACCATGGTAA

Coding sequence (CDS)

ATGGCAATGGCTCAATCAGCAACTGAATTTGCATGGGGAAATGCTCTAAAAAAGAAGCTTCTAATTCAAAGACAAGAAGTTTGCAATAATGGTTTTGATTTTCCTCTCCAAACAGTGAAATTTTTTCAAGATAAAAATTTAGAAAGAGAAGAAGAAGGAGAAGAGAAAAAGATGGAAAATGGATTGATGAAGCTGAGAAAAATCTTACCAGGTGGTAATGGTGATTCTAATGGATATTTGGAAGAAGATGATTTGTTGAAACAAACTGAAAGCTATATAAAATGTCTTGAATTGCAGATGAAAGATAGATCCTGGTACTACAAAAACAATTCTAGATATGGTGTTGATCAGAGACCATGGTAA

Protein sequence

MAMAQSATEFAWGNALKKKLLIQRQEVCNNGFDFPLQTVKFFQDKNLEREEEGEEKKMENGLMKLRKILPGGNGDSNGYLEEDDLLKQTESYIKCLELQMKDRSWYYKNNSRYGVDQRPW
Homology
BLAST of Sed0008313 vs. NCBI nr
Match: XP_023538247.1 (transcription factor bHLH146-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 131.3 bits (329), Expect = 5.4e-27
Identity = 75/104 (72.12%), Postives = 83/104 (79.81%), Query Frame = 0

Query: 1   MAMAQSATEFAWGNALKKKLLIQRQEVCNNGFDFPLQTVKFFQDKNLERE----EEGEEK 60
           MAMAQSATEF+WGNALKKKLL +     +NGF F LQTVKF Q+ NLERE    EE +E 
Sbjct: 62  MAMAQSATEFSWGNALKKKLLQREDGNGSNGFGFSLQTVKFSQE-NLEREHEEHEERKEP 121

Query: 61  KMENGLMKLRKILPGGNGDSNGYLEEDDLLKQTESYIKCLELQM 101
           K+ENGLMKLRKI+PGG   SNG LEEDDL KQTESYIKCLELQ+
Sbjct: 122 KIENGLMKLRKIVPGG---SNGELEEDDLFKQTESYIKCLELQV 161

BLAST of Sed0008313 vs. NCBI nr
Match: XP_022996791.1 (transcription factor bHLH146-like [Cucurbita maxima])

HSP 1 Score: 129.4 bits (324), Expect = 2.1e-26
Identity = 74/104 (71.15%), Postives = 84/104 (80.77%), Query Frame = 0

Query: 1   MAMAQSATEFAWGNALKKKLLIQRQEVCNNGFDFPLQTVKFFQDKNLEREEEGEEKKMEN 60
           MAMA+SAT+FAWGNALKKKLL   Q    NGFDF LQT+KF ++ NLEREEE EE+KMEN
Sbjct: 54  MAMAESATQFAWGNALKKKLL---QTKVANGFDFSLQTLKFSRE-NLEREEE-EEEKMEN 113

Query: 61  GLMKLRKILPGGNGDS----NGYLEEDDLLKQTESYIKCLELQM 101
           GL KLRKI+PGG G      NG LEE++LLKQTESYIKCLELQ+
Sbjct: 114 GLKKLRKIIPGGGGGGGGGFNGGLEEEELLKQTESYIKCLELQV 152

BLAST of Sed0008313 vs. NCBI nr
Match: XP_022929709.1 (transcription factor bHLH146-like [Cucurbita moschata])

HSP 1 Score: 129.0 bits (323), Expect = 2.7e-26
Identity = 73/102 (71.57%), Postives = 84/102 (82.35%), Query Frame = 0

Query: 1   MAMAQSATEFAWGNALKKKLLIQRQEVCNNGFDFPLQTVKFFQDKNLEREEEGEEKKMEN 60
           MAMA+SAT+FAWGNALKKKLL   Q    NGFDF LQT+KF ++ NLEREEE EE+KMEN
Sbjct: 54  MAMAESATQFAWGNALKKKLL---QTKVANGFDFSLQTLKFSRE-NLEREEE-EEEKMEN 113

Query: 61  GLMKLRKILPGGNGDS--NGYLEEDDLLKQTESYIKCLELQM 101
           GL +LRKI+PGG G    NG LEE++LLKQTESYIKCLELQ+
Sbjct: 114 GLKRLRKIIPGGGGGGGFNGGLEEEELLKQTESYIKCLELQV 150

BLAST of Sed0008313 vs. NCBI nr
Match: KAG6598877.1 (Transcription factor IBH1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 129.0 bits (323), Expect = 2.7e-26
Identity = 73/102 (71.57%), Postives = 84/102 (82.35%), Query Frame = 0

Query: 1   MAMAQSATEFAWGNALKKKLLIQRQEVCNNGFDFPLQTVKFFQDKNLEREEEGEEKKMEN 60
           MAMA+SAT+FAWGNALKKKLL   Q    NGFDF LQT+KF ++ NLEREEE EE+KMEN
Sbjct: 1   MAMAESATQFAWGNALKKKLL---QTKVANGFDFSLQTLKFSRE-NLEREEE-EEEKMEN 60

Query: 61  GLMKLRKILPGGNGDS--NGYLEEDDLLKQTESYIKCLELQM 101
           GL +LRKI+PGG G    NG LEE++LLKQTESYIKCLELQ+
Sbjct: 61  GLKRLRKIIPGGGGGGGLNGGLEEEELLKQTESYIKCLELQV 97

BLAST of Sed0008313 vs. NCBI nr
Match: XP_022153354.1 (transcription factor bHLH146 [Momordica charantia])

HSP 1 Score: 127.9 bits (320), Expect = 6.0e-26
Identity = 76/107 (71.03%), Postives = 87/107 (81.31%), Query Frame = 0

Query: 1   MAMAQSATEFAWGNALKKKLLIQRQEVCN----NGFDFPLQTVKFFQDKNLEREEEGEEK 60
           MAMA SA+EFAWG+ALK+KLL Q+QE  N    N FDF LQT+KF ++K  E EEE ++K
Sbjct: 57  MAMALSASEFAWGHALKQKLL-QKQENGNGNGRNSFDFSLQTLKFSREK--EEEEEEDKK 116

Query: 61  KMENGLMKLRKILPGG--NGDSNG-YLEEDDLLKQTESYIKCLELQM 101
           KMENGL+KLRKILPGG  N DSNG  LEEDDLLKQTESYIKCLELQ+
Sbjct: 117 KMENGLVKLRKILPGGENNNDSNGDLLEEDDLLKQTESYIKCLELQV 160

BLAST of Sed0008313 vs. ExPASy Swiss-Prot
Match: Q9SUM5 (Transcription factor bHLH146 OS=Arabidopsis thaliana OX=3702 GN=BHLH146 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 7.9e-05
Identity = 39/104 (37.50%), Postives = 55/104 (52.88%), Query Frame = 0

Query: 1   MAMAQSATEFAWGNALKKKLLIQRQEVCNNGFDFPLQTVKFFQD--KNLER-EEEG--EE 60
           MA+A SA EFAW   L +KL         +    P  T     D  + LER ++EG  EE
Sbjct: 57  MALALSAQEFAWSRFLLQKL---------SSSSNPTTTTSSSSDGIRILERPDKEGGNEE 116

Query: 61  KKMENGLMKLRKILPGGNGDSNGYLEEDDLLKQTESYIKCLELQ 100
             +E  L +L+K+LPGG       +  +++L +  +YIKCLELQ
Sbjct: 117 GGIEERLRELKKLLPGGE-----EMNVEEMLSEIGNYIKCLELQ 146

BLAST of Sed0008313 vs. ExPASy TrEMBL
Match: A0A6J1K5R7 (transcription factor bHLH146-like OS=Cucurbita maxima OX=3661 GN=LOC111491923 PE=4 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.0e-26
Identity = 74/104 (71.15%), Postives = 84/104 (80.77%), Query Frame = 0

Query: 1   MAMAQSATEFAWGNALKKKLLIQRQEVCNNGFDFPLQTVKFFQDKNLEREEEGEEKKMEN 60
           MAMA+SAT+FAWGNALKKKLL   Q    NGFDF LQT+KF ++ NLEREEE EE+KMEN
Sbjct: 54  MAMAESATQFAWGNALKKKLL---QTKVANGFDFSLQTLKFSRE-NLEREEE-EEEKMEN 113

Query: 61  GLMKLRKILPGGNGDS----NGYLEEDDLLKQTESYIKCLELQM 101
           GL KLRKI+PGG G      NG LEE++LLKQTESYIKCLELQ+
Sbjct: 114 GLKKLRKIIPGGGGGGGGGFNGGLEEEELLKQTESYIKCLELQV 152

BLAST of Sed0008313 vs. ExPASy TrEMBL
Match: A0A6J1EPJ9 (transcription factor bHLH146-like OS=Cucurbita moschata OX=3662 GN=LOC111436209 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 1.3e-26
Identity = 73/102 (71.57%), Postives = 84/102 (82.35%), Query Frame = 0

Query: 1   MAMAQSATEFAWGNALKKKLLIQRQEVCNNGFDFPLQTVKFFQDKNLEREEEGEEKKMEN 60
           MAMA+SAT+FAWGNALKKKLL   Q    NGFDF LQT+KF ++ NLEREEE EE+KMEN
Sbjct: 54  MAMAESATQFAWGNALKKKLL---QTKVANGFDFSLQTLKFSRE-NLEREEE-EEEKMEN 113

Query: 61  GLMKLRKILPGGNGDS--NGYLEEDDLLKQTESYIKCLELQM 101
           GL +LRKI+PGG G    NG LEE++LLKQTESYIKCLELQ+
Sbjct: 114 GLKRLRKIIPGGGGGGGFNGGLEEEELLKQTESYIKCLELQV 150

BLAST of Sed0008313 vs. ExPASy TrEMBL
Match: A0A6J1DIU0 (transcription factor bHLH146 OS=Momordica charantia OX=3673 GN=LOC111020869 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.9e-26
Identity = 76/107 (71.03%), Postives = 87/107 (81.31%), Query Frame = 0

Query: 1   MAMAQSATEFAWGNALKKKLLIQRQEVCN----NGFDFPLQTVKFFQDKNLEREEEGEEK 60
           MAMA SA+EFAWG+ALK+KLL Q+QE  N    N FDF LQT+KF ++K  E EEE ++K
Sbjct: 57  MAMALSASEFAWGHALKQKLL-QKQENGNGNGRNSFDFSLQTLKFSREK--EEEEEEDKK 116

Query: 61  KMENGLMKLRKILPGG--NGDSNG-YLEEDDLLKQTESYIKCLELQM 101
           KMENGL+KLRKILPGG  N DSNG  LEEDDLLKQTESYIKCLELQ+
Sbjct: 117 KMENGLVKLRKILPGGENNNDSNGDLLEEDDLLKQTESYIKCLELQV 160

BLAST of Sed0008313 vs. ExPASy TrEMBL
Match: A0A6J1FJ33 (transcription factor bHLH146-like OS=Cucurbita moschata OX=3662 GN=LOC111444354 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 6.5e-26
Identity = 73/107 (68.22%), Postives = 82/107 (76.64%), Query Frame = 0

Query: 1   MAMAQSATEFAWGNALKKKLLIQRQEVCNNGFDFPLQTVKFFQDKNLERE-------EEG 60
           MAMAQSATEF+WGNALKKKLL +     +NGF   LQ VKF ++ NLERE       EE 
Sbjct: 62  MAMAQSATEFSWGNALKKKLLQREDGNGSNGFGLSLQRVKFSRE-NLEREDEEHEEREEH 121

Query: 61  EEKKMENGLMKLRKILPGGNGDSNGYLEEDDLLKQTESYIKCLELQM 101
           EE+K+ENGLMKLRKI+PGG   SNG LEEDDL KQTESYIKCLELQ+
Sbjct: 122 EERKIENGLMKLRKIVPGG---SNGELEEDDLFKQTESYIKCLELQV 164

BLAST of Sed0008313 vs. ExPASy TrEMBL
Match: A0A6J1I0E6 (transcription factor bHLH146-like OS=Cucurbita maxima OX=3661 GN=LOC111468586 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 7.1e-25
Identity = 73/110 (66.36%), Postives = 83/110 (75.45%), Query Frame = 0

Query: 1   MAMAQSATEFAWGNALKKKLLIQRQEVCNNGFDFPLQTVKFFQDKNLERE---------- 60
           MAMAQSATEF+WGNALKKKLL +  +  +NGF F L  VKF ++ NLERE          
Sbjct: 62  MAMAQSATEFSWGNALKKKLLQREDDNGSNGFGFSL-PVKFSRE-NLEREDEEHEEHEEH 121

Query: 61  EEGEEKKMENGLMKLRKILPGGNGDSNGYLEEDDLLKQTESYIKCLELQM 101
           EE EE+K+ENGLMKLRKI+PGG   SNG LEEDDL KQTESYIKCLELQ+
Sbjct: 122 EEREERKIENGLMKLRKIVPGG---SNGELEEDDLFKQTESYIKCLELQV 166

BLAST of Sed0008313 vs. TAIR 10
Match: AT2G18969.1 (BEST Arabidopsis thaliana protein match is: sequence-specific DNA binding transcription factors;transcription regulators (TAIR:AT4G30180.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 51.2 bits (121), Expect = 6.6e-07
Identity = 37/112 (33.04%), Postives = 59/112 (52.68%), Query Frame = 0

Query: 1   MAMAQSATEFAWGNALKKKLLIQRQEVCNNGFDFPLQT------------VKFFQDKNLE 60
           MA A SA EFAW   L++KLL       ++ +D P+ T             +   +K+ +
Sbjct: 64  MAFALSAQEFAWSRFLQQKLL-------SSPYDDPISTSSSPSEILERSSKRQGGEKHQD 123

Query: 61  REEEGEEKKMENGLMKLRKILPGGNGDSNGYLEEDDLLKQTESYIKCLELQM 101
            +EE E  +++  L +L+K+LPGG       +  +++L +  SYI CLELQM
Sbjct: 124 SDEEEEGGEIKKRLKELQKLLPGGE-----EMNMEEILSEIGSYIVCLELQM 163

BLAST of Sed0008313 vs. TAIR 10
Match: AT4G30180.1 (sequence-specific DNA binding transcription factors;transcription regulators )

HSP 1 Score: 48.1 bits (113), Expect = 5.6e-06
Identity = 39/104 (37.50%), Postives = 55/104 (52.88%), Query Frame = 0

Query: 1   MAMAQSATEFAWGNALKKKLLIQRQEVCNNGFDFPLQTVKFFQD--KNLER-EEEG--EE 60
           MA+A SA EFAW   L +KL         +    P  T     D  + LER ++EG  EE
Sbjct: 57  MALALSAQEFAWSRFLLQKL---------SSSSNPTTTTSSSSDGIRILERPDKEGGNEE 116

Query: 61  KKMENGLMKLRKILPGGNGDSNGYLEEDDLLKQTESYIKCLELQ 100
             +E  L +L+K+LPGG       +  +++L +  +YIKCLELQ
Sbjct: 117 GGIEERLRELKKLLPGGE-----EMNVEEMLSEIGNYIKCLELQ 146

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023538247.15.4e-2772.12transcription factor bHLH146-like [Cucurbita pepo subsp. pepo][more]
XP_022996791.12.1e-2671.15transcription factor bHLH146-like [Cucurbita maxima][more]
XP_022929709.12.7e-2671.57transcription factor bHLH146-like [Cucurbita moschata][more]
KAG6598877.12.7e-2671.57Transcription factor IBH1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022153354.16.0e-2671.03transcription factor bHLH146 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9SUM57.9e-0537.50Transcription factor bHLH146 OS=Arabidopsis thaliana OX=3702 GN=BHLH146 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1K5R71.0e-2671.15transcription factor bHLH146-like OS=Cucurbita maxima OX=3661 GN=LOC111491923 PE... [more]
A0A6J1EPJ91.3e-2671.57transcription factor bHLH146-like OS=Cucurbita moschata OX=3662 GN=LOC111436209 ... [more]
A0A6J1DIU02.9e-2671.03transcription factor bHLH146 OS=Momordica charantia OX=3673 GN=LOC111020869 PE=4... [more]
A0A6J1FJ336.5e-2668.22transcription factor bHLH146-like OS=Cucurbita moschata OX=3662 GN=LOC111444354 ... [more]
A0A6J1I0E67.1e-2566.36transcription factor bHLH146-like OS=Cucurbita maxima OX=3661 GN=LOC111468586 PE... [more]
Match NameE-valueIdentityDescription
AT2G18969.16.6e-0733.04BEST Arabidopsis thaliana protein match is: sequence-specific DNA binding transc... [more]
AT4G30180.15.6e-0637.50sequence-specific DNA binding transcription factors;transcription regulators [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33124:SF42TRANSCRIPTION FACTOR BHLH146coord: 1..33
NoneNo IPR availablePANTHERPTHR33124:SF42TRANSCRIPTION FACTOR BHLH146coord: 44..101
IPR044660Transcription factor IBH1-likePANTHERPTHR33124TRANSCRIPTION FACTOR IBH1-LIKE 1coord: 44..101
IPR044660Transcription factor IBH1-likePANTHERPTHR33124TRANSCRIPTION FACTOR IBH1-LIKE 1coord: 1..33

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0008313.1Sed0008313.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0046983 protein dimerization activity