Sed0008308 (gene) Chayote v1

Overview
NameSed0008308
Typegene
OrganismSechium edule (Chayote v1)
DescriptionRING-type E3 ubiquitin transferase
LocationLG03: 5410219 .. 5413010 (-)
RNA-Seq ExpressionSed0008308
SyntenySed0008308
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTTCTTAAGCGAAAAAAAGGTTGTAAACAGTTTTATCTCTGAATAATCGGATATTCTTAAATAATAATTAAAAAAATCTCGAAATAATCGGGTGGCGTGCAATATTGGAGAGTAATGGTTAGAGCATACGTGGCGATCTTGCGGATAACAACGGTCTTCTTCCTCGCGAGGAGAAAGGAGAATCGATAATGGCGAAGTTTTCGGTTGACGGAGAAGAGGACGGCGAGGACGGCGGAGCCGGCACCAGCAACCCTAGGGCAAAGAGGAAGAGGACAACCACCGTTCCGATCAACCCCGGTTTTCCCCCACCCCGTGAACCGGACCGACAAGAAACCGCCAACGATCATCAACACTACCAGACCAACCGCCGTCGACATGACGAACCGCTTCCAGAGGAAGATTCTGAAACACCGATTTGGTCGCCCAAGTCCCGTGCTAAAAATGTCAAGGCGAATAGCTCCGTTTTGATGTTTTTGATTGACCCAGAAGTTTTTGATTGCGCCATTTGCTGCGAACCCTTGACCCTCCCCATATTCCAGGTCTGTTTCCATACTTGCTCTGTTCTTGCTTAATTGTAATTTTATTAGTTGACTGTGTGTTTGATCCTTAATTGGCTTTTAGTTTGTTCGTCCTGTATAAAGGGGTTTTCCCCCAAAATCATTTAGATCCCTAAATTTGATCGATCAAGCTAAAGGGTATTGGTTAGGTTGATTTGGGTTTACTTTAGTTTTATTGACCCTTTCATCCGATAAGATGAACCAAATATACCACCACTGTAGTGTTATGGTTGAGAAACGTTTGTTGTTTCTCAGACCATTAGAAGATAGAGTCAAATACCTTATCAAGTTTGTCCACTACCTTCTGATCATAGGACTGATCATTGAATTGAGGACACTAATAATGAACAACATTCTTAACATCTAGTTGGGGTACCAACTAATTAGAATAGGTAGCAAGAGTAAGAGTGACAGTTGTAGTTTGTTCTTAATCATTGGGTTCATCAAAGCCATAGCCTATTGGAAAGATTGCCAGTGAGAAGGAACGATTGGATGAATGGTATGTACCATTGTTGTTAGGAGACTTTGTTTTATTGATTATTCGGTCATCATCTTTGAGGCACTTAGAGAGATGATCCAGGAAGATTTTTGCTCAATCCTTTTAATGCAAAGGAAGCATTGTCTAGAGGGGTTAACAAACATGCTTGCAGTAGATCCTTGTAAGTTTGTTTAGTATATTTGAGAGCATGGAGGTTATAACACATATGGGGGTTAGATTGTGGTAAGTGATCTTTATTATCATTGTGGGAAAAGGAGATATTTTGAAAAAATGGGAAAAATTTGTGGGGGGTTTTAATCAATGTGGTGCAAAAACTATTAACACACCTGATTACTTAGAATAGTGCTTAGAGATAAGAGGTAATTTTTGTTGGTTTATCCAGCCAAAATTGAAATTGAACAACCAAGGGAATAGAGATTTTTGGAGCAAGCTGTGCTTGGCCATTCTTCAAGTCTCTTGGCTAGCGGGGCTGCTTAGGATTTCAGCCATAGCAACTACGTAGTATTCCTTCATGTATAATTGGTGTTTAATGTAGATTTGCGCTTTGGAAGTAAAAGGAGAACGAAAGTTTTCTTATAAGAAAATTTTGATTGATCAGCTAACATCTGCTAATTTCCAGTAAATTATTCAGTTTTGGTGTGATCTGGTAGCTCCCTCTTTAACTTCCGCCAATTATTTCAGTTATATTGATCACAGTTAGTGCGAACTGGACATGATTGTTTAATGATTGTCTGTTTTATTTTGTCATGTCATCACATATATTAACAGTAACGAGGCTTAGCTAGACTTTTTATCATGAATTCAGTGTGAGAATGGGCATATTGCCTGCTCTTCCTGCTGCATCAAAATTAAGAATAAATGTGCAACCTGCTCTTGGCCTATTGGCTATAATCGTTGTCGGGCGGTTGAGAAGGTTCTTGAATCCATTAAACTACCCTGCAAACAATCGAAGTATGGATGCAAGGAGATAGTTGGTTATGGTAACGAAAGTGACCACGAAGAGACATGCCTTTACACTCCTTGTAAATGCCCCCTTTCGGATTGCAACTTTATGGCCTCATCCAAACAGCTATCATTACACTTCAGCAGTGAACATACAGACTCTGCTACCAACTTCCAGTTCAGTTCTTGCTTCAACATAATTTGCTTAATAAATGACGATAGTTACGACGTTCTTCAAGAACAAGATGATGGCATTCTTTTTATTCTCAGTCATAGTTATGAACTTCAGGGAAATGCTGTCAAAATTTGTTGCCTAAGACCTCCTACTTCGAAAGAAGCATTTTCTTATTCTATACGTGTAGAAACTCAGGATCTCTCTCTTGTTCTACAATCTTCTATGAAGAACATTCGGTCCTCCTCGGATTTCTGTCGCTCGACAGGGTTCCTTTTGATTCCACGTGATGTATTTGGTACTTCTGGCAAGGTCAAGTTGAATGTCTGCATACAGAAAAACGGTTCGTCTCCAGTGTCCGATAGATAAAAGGCGTCACGGACATGTCAACAAGTATATATGGTGCTGTTTGTTTCAATTTGTATATCAGTGTGCCTAGTTCATATATGATATAAAGTAAGGTGATCTTAGAAGCTCAGTTATGGTGCATAATTCTGTATATAGTAATTTCTCATTTTGCTTAGTTTATTATGATTTGTACCGTTGGTGGTAAACACTCTGCATTGTGTTATGTTGGAAAACTCTTCTGTGGCTGAATAATGAGGAGCTATCTACACACA

mRNA sequence

TTCTTCTTAAGCGAAAAAAAGGTTGTAAACAGTTTTATCTCTGAATAATCGGATATTCTTAAATAATAATTAAAAAAATCTCGAAATAATCGGGTGGCGTGCAATATTGGAGAGTAATGGTTAGAGCATACGTGGCGATCTTGCGGATAACAACGGTCTTCTTCCTCGCGAGGAGAAAGGAGAATCGATAATGGCGAAGTTTTCGGTTGACGGAGAAGAGGACGGCGAGGACGGCGGAGCCGGCACCAGCAACCCTAGGGCAAAGAGGAAGAGGACAACCACCGTTCCGATCAACCCCGGTTTTCCCCCACCCCGTGAACCGGACCGACAAGAAACCGCCAACGATCATCAACACTACCAGACCAACCGCCGTCGACATGACGAACCGCTTCCAGAGGAAGATTCTGAAACACCGATTTGGTCGCCCAAGTCCCGTGCTAAAAATGTCAAGGCGAATAGCTCCGTTTTGATGTTTTTGATTGACCCAGAAGTTTTTGATTGCGCCATTTGCTGCGAACCCTTGACCCTCCCCATATTCCAGTGTGAGAATGGGCATATTGCCTGCTCTTCCTGCTGCATCAAAATTAAGAATAAATGTGCAACCTGCTCTTGGCCTATTGGCTATAATCGTTGTCGGGCGGTTGAGAAGGTTCTTGAATCCATTAAACTACCCTGCAAACAATCGAAGTATGGATGCAAGGAGATAGTTGGTTATGGTAACGAAAGTGACCACGAAGAGACATGCCTTTACACTCCTTGTAAATGCCCCCTTTCGGATTGCAACTTTATGGCCTCATCCAAACAGCTATCATTACACTTCAGCAGTGAACATACAGACTCTGCTACCAACTTCCAGTTCAGTTCTTGCTTCAACATAATTTGCTTAATAAATGACGATAGTTACGACGTTCTTCAAGAACAAGATGATGGCATTCTTTTTATTCTCAGTCATAGTTATGAACTTCAGGGAAATGCTGTCAAAATTTGTTGCCTAAGACCTCCTACTTCGAAAGAAGCATTTTCTTATTCTATACGTGTAGAAACTCAGGATCTCTCTCTTGTTCTACAATCTTCTATGAAGAACATTCGGTCCTCCTCGGATTTCTGTCGCTCGACAGGGTTCCTTTTGATTCCACGTGATGTATTTGGTACTTCTGGCAAGGTCAAGTTGAATGTCTGCATACAGAAAAACGGTTCGTCTCCAGTGTCCGATAGATAAAAGGCGTCACGGACATGTCAACAAGTATATATGGTGCTGTTTGTTTCAATTTGTATATCAGTGTGCCTAGTTCATATATGATATAAAGTAAGGTGATCTTAGAAGCTCAGTTATGGTGCATAATTCTGTATATAGTAATTTCTCATTTTGCTTAGTTTATTATGATTTGTACCGTTGGTGGTAAACACTCTGCATTGTGTTATGTTGGAAAACTCTTCTGTGGCTGAATAATGAGGAGCTATCTACACACA

Coding sequence (CDS)

ATGGCGAAGTTTTCGGTTGACGGAGAAGAGGACGGCGAGGACGGCGGAGCCGGCACCAGCAACCCTAGGGCAAAGAGGAAGAGGACAACCACCGTTCCGATCAACCCCGGTTTTCCCCCACCCCGTGAACCGGACCGACAAGAAACCGCCAACGATCATCAACACTACCAGACCAACCGCCGTCGACATGACGAACCGCTTCCAGAGGAAGATTCTGAAACACCGATTTGGTCGCCCAAGTCCCGTGCTAAAAATGTCAAGGCGAATAGCTCCGTTTTGATGTTTTTGATTGACCCAGAAGTTTTTGATTGCGCCATTTGCTGCGAACCCTTGACCCTCCCCATATTCCAGTGTGAGAATGGGCATATTGCCTGCTCTTCCTGCTGCATCAAAATTAAGAATAAATGTGCAACCTGCTCTTGGCCTATTGGCTATAATCGTTGTCGGGCGGTTGAGAAGGTTCTTGAATCCATTAAACTACCCTGCAAACAATCGAAGTATGGATGCAAGGAGATAGTTGGTTATGGTAACGAAAGTGACCACGAAGAGACATGCCTTTACACTCCTTGTAAATGCCCCCTTTCGGATTGCAACTTTATGGCCTCATCCAAACAGCTATCATTACACTTCAGCAGTGAACATACAGACTCTGCTACCAACTTCCAGTTCAGTTCTTGCTTCAACATAATTTGCTTAATAAATGACGATAGTTACGACGTTCTTCAAGAACAAGATGATGGCATTCTTTTTATTCTCAGTCATAGTTATGAACTTCAGGGAAATGCTGTCAAAATTTGTTGCCTAAGACCTCCTACTTCGAAAGAAGCATTTTCTTATTCTATACGTGTAGAAACTCAGGATCTCTCTCTTGTTCTACAATCTTCTATGAAGAACATTCGGTCCTCCTCGGATTTCTGTCGCTCGACAGGGTTCCTTTTGATTCCACGTGATGTATTTGGTACTTCTGGCAAGGTCAAGTTGAATGTCTGCATACAGAAAAACGGTTCGTCTCCAGTGTCCGATAGATAA

Protein sequence

MAKFSVDGEEDGEDGGAGTSNPRAKRKRTTTVPINPGFPPPREPDRQETANDHQHYQTNRRRHDEPLPEEDSETPIWSPKSRAKNVKANSSVLMFLIDPEVFDCAICCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHTDSATNFQFSSCFNIICLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPTSKEAFSYSIRVETQDLSLVLQSSMKNIRSSSDFCRSTGFLLIPRDVFGTSGKVKLNVCIQKNGSSPVSDR
Homology
BLAST of Sed0008308 vs. NCBI nr
Match: XP_023534163.1 (E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 465.7 bits (1197), Expect = 3.5e-127
Identity = 243/356 (68.26%), Postives = 283/356 (79.49%), Query Frame = 0

Query: 1   MAKFSVDGEEDGEDGGAGTSNPRAKRKRTTTVPINPGFPPPREPDR-------------Q 60
           MAKFSV GEEDGED G+ TS+PRAKRKR T+  IN     PR   R             +
Sbjct: 30  MAKFSVGGEEDGED-GSSTSSPRAKRKR-TSFRINAVVNFPRHNQRCEEGEEQGFDYCKE 89

Query: 61  ETANDHQHYQTNRRRH-----DEPLPEEDSETPIWSPKSRAKNVKANSSVLMFLIDPEVF 120
           ETA+D +     R+RH     +E + E+ +E  + S KS +KNVK N S+ MFLIDPEV 
Sbjct: 90  ETASDEE----QRKRHRIDGIEEQVGEDGTEKALCSVKSCSKNVKENGSISMFLIDPEVL 149

Query: 121 DCAICCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVLESIKLPC 180
           DC+ICCEPL++PIFQCENGHIACSSCC K+KN+CATCSWPIGYNRCRAVEKVLESIKLPC
Sbjct: 150 DCSICCEPLSVPIFQCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLESIKLPC 209

Query: 181 KQSKYGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHTDSATNFQ 240
           KQSKYGCKEIV YG+ESDHE+ CL+ PCKCPLSDCNF+ASSKQLSLHFSS+HTDSA +FQ
Sbjct: 210 KQSKYGCKEIVSYGSESDHEKKCLHAPCKCPLSDCNFVASSKQLSLHFSSKHTDSAISFQ 269

Query: 241 FSSCFNIICLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPTSKEAFSYSIR 300
           FSS F  ICL  +D+Y VLQEQ DGILFILS+S++  GNAVK+CCLRPP+ KEAFSY +R
Sbjct: 270 FSSSFT-ICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPSLKEAFSYDLR 329

Query: 301 VETQDLSLVLQSSMKNIRSSSDFCRSTGFLLIPRDVFGTSGKVKLNVCIQKNGSSP 339
           VETQDLSLVLQSSMKNI+SSSD C ST FLLIPRD+F +SGK+KLN+CI +N  +P
Sbjct: 330 VETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSGKIKLNICIWQNTGAP 378

BLAST of Sed0008308 vs. NCBI nr
Match: XP_022956630.1 (E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita moschata] >KAG6602037.1 E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 459.9 bits (1182), Expect = 1.9e-125
Identity = 239/356 (67.13%), Postives = 281/356 (78.93%), Query Frame = 0

Query: 1   MAKFSVDGEEDGEDGGAGTSNPRAKRKRTTTVPINPGFPPPREPDR-------------Q 60
           MAKFSV GEEDGED G+ TS+PRAKRKR T+  IN     PR   R             +
Sbjct: 1   MAKFSVGGEEDGED-GSSTSSPRAKRKR-TSFRINAVVNFPRHNQRCEEGEEQGFDYCKE 60

Query: 61  ETANDHQHYQTNRRRH-----DEPLPEEDSETPIWSPKSRAKNVKANSSVLMFLIDPEVF 120
           ETA+D +     R+RH     +E + E+ +E  + S KS ++NVK N S+ MFLIDPEV 
Sbjct: 61  ETASDEE----QRKRHRIDGIEEQVEEDGTENALCSVKSCSRNVKENGSISMFLIDPEVL 120

Query: 121 DCAICCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVLESIKLPC 180
           DC+ICCEPL++PIFQCENGHIACSSCC K+KN+CATCSWPIGYNRCRAVEKVLESIKLPC
Sbjct: 121 DCSICCEPLSVPIFQCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLESIKLPC 180

Query: 181 KQSKYGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHTDSATNFQ 240
           KQSKYGCKE+V YG+ESDHE+ CL+ PCKCPL DCNF+ASSKQLSLHFSS+HTDSA +FQ
Sbjct: 181 KQSKYGCKEMVSYGSESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFSSKHTDSAISFQ 240

Query: 241 FSSCFNIICLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPTSKEAFSYSIR 300
           FSS F  ICL  +D+Y VLQEQ DGILFILS+S++  GNAVK+CCLRPP+ KEAFSY +R
Sbjct: 241 FSSSFT-ICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPSLKEAFSYDLR 300

Query: 301 VETQDLSLVLQSSMKNIRSSSDFCRSTGFLLIPRDVFGTSGKVKLNVCIQKNGSSP 339
           VETQDLSLVLQSSMKNI+SSSD C ST FLLIPRD+F +SG +KLN+CI +N  +P
Sbjct: 301 VETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICIWQNTGAP 349

BLAST of Sed0008308 vs. NCBI nr
Match: XP_038874338.1 (putative E3 ubiquitin-protein ligase SINA-like 6 [Benincasa hispida])

HSP 1 Score: 458.4 bits (1178), Expect = 5.5e-125
Identity = 237/356 (66.57%), Postives = 276/356 (77.53%), Query Frame = 0

Query: 1   MAKFSVDGEEDGEDGGAGTSNPRAKRKRTT-TVPINPGFPPPRE-----------PDRQE 60
           MAKFSVDGE   EDGG G S+PR KRKR++  +     FP   E            D++E
Sbjct: 1   MAKFSVDGE---EDGGDGPSSPRGKRKRSSFRINATVDFPRHNEQREQGEQQGFNSDKEE 60

Query: 61  TANDHQHYQTNRRRHDEPLPEED--SETPIWSPKSRAKNVKANSSVLMFLIDPEVFDCAI 120
           + ND +  + +R    E    +D  S+  + S KS  KNVK N SV MFLIDPEV DC+I
Sbjct: 61  SENDEEETKRHRIHEIEEHARQDDGSKRSLCSVKSGTKNVKPNGSVSMFLIDPEVLDCSI 120

Query: 121 CCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVLESIKLPCKQSK 180
           CCEPL++PIFQCENGHIACSSCC K+KNKCATCSWPIGYNRCRAVEKVLESIK PCKQSK
Sbjct: 121 CCEPLSIPIFQCENGHIACSSCCNKLKNKCATCSWPIGYNRCRAVEKVLESIKFPCKQSK 180

Query: 181 YGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHTDSATNFQFSSC 240
           +GCKEIVGYGNESDHE+TCLY PC CP SDCNFMASSKQLSLHFS++HT+SATNFQF+S 
Sbjct: 181 FGCKEIVGYGNESDHEKTCLYAPCLCPHSDCNFMASSKQLSLHFSNKHTNSATNFQFNSS 240

Query: 241 FNIICLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPTSKEAFSYSIRVETQ 300
           F  ICL  +++Y VLQEQDDGILFILS+S+E  G+ VKICCLRP + KEAFSY +R ETQ
Sbjct: 241 FT-ICLKTNNTYRVLQEQDDGILFILSNSFENLGSVVKICCLRPSSLKEAFSYDLRAETQ 300

Query: 301 DLSLVLQSSMKNIRSSSDFCRSTGFLLIPRDVFGTSGKVKLNVCIQKNGSSPVSDR 343
           DLSLVLQSSMKNI+SSSD C ST FL+IPRD+F +SG ++LN+CI +NGS PV  R
Sbjct: 301 DLSLVLQSSMKNIQSSSDCCHSTRFLMIPRDLFCSSGMIRLNICIWQNGSPPVRHR 352

BLAST of Sed0008308 vs. NCBI nr
Match: XP_008460511.1 (PREDICTED: E3 ubiquitin-protein ligase SINA-like 7 [Cucumis melo])

HSP 1 Score: 455.7 bits (1171), Expect = 3.6e-124
Identity = 236/357 (66.11%), Postives = 275/357 (77.03%), Query Frame = 0

Query: 1   MAKFSVDGEEDGEDGGAGTSNPRAKRKRTT-TVPINPGFPPPRE-----------PDRQE 60
           MAKFSVDGE   ED G G S+PR KRKR++  +     FP   E            D++E
Sbjct: 1   MAKFSVDGE---EDAGDGPSSPRGKRKRSSFRINATVDFPRRSEQREQGEEQGFNSDKEE 60

Query: 61  TANDHQHYQTNRRRHD---EPLPEEDSETPIWSPKSRAKNVKANSSVLMFLIDPEVFDCA 120
           + ND    Q+ R R+D   E L E+  +  +WS KS  KN K N SV MFLIDPEV DC+
Sbjct: 61  SENDED--QSKRHRNDEIEEHLREDGGKRALWSGKSCTKNAKPNGSVSMFLIDPEVLDCS 120

Query: 121 ICCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVLESIKLPCKQS 180
           ICCEPL++PIFQCENGHIACSSCC K+KNKCATCSWPIGYNRCRAVEKVLESIKLPCKQS
Sbjct: 121 ICCEPLSIPIFQCENGHIACSSCCNKLKNKCATCSWPIGYNRCRAVEKVLESIKLPCKQS 180

Query: 181 KYGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHTDSATNFQFSS 240
           K+GCKEI+GYGNESDHE+TCLY PC CP SDC F+AS+KQLSLHFS++HT+SATNF FSS
Sbjct: 181 KFGCKEIIGYGNESDHEKTCLYAPCLCPYSDCKFIASAKQLSLHFSNKHTNSATNFHFSS 240

Query: 241 CFNIICLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPTSKEAFSYSIRVET 300
            F  ICL  +D+Y VLQEQ DG+LFILS+S E  G+ VKICCL+PP+ KE FSY +R ET
Sbjct: 241 SFT-ICLKTNDTYHVLQEQ-DGVLFILSNSSENLGSVVKICCLQPPSLKETFSYDLRAET 300

Query: 301 QDLSLVLQSSMKNIRSSSDFCRSTGFLLIPRDVFGTSGKVKLNVCIQKNGSSPVSDR 343
           QDLSLVLQSSMKNI+SSSD C ST FL+IPRD+F +SG +KLN+CI +NGSSPV  R
Sbjct: 301 QDLSLVLQSSMKNIQSSSDCCHSTRFLMIPRDLFSSSGMIKLNICIWQNGSSPVHHR 350

BLAST of Sed0008308 vs. NCBI nr
Match: XP_022990964.1 (E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima])

HSP 1 Score: 454.1 bits (1167), Expect = 1.0e-123
Identity = 238/354 (67.23%), Postives = 280/354 (79.10%), Query Frame = 0

Query: 1   MAKFSVDGEEDGEDGGAGTSNPRAKRKRTTTVPINPGFPPPREPDRQETANDHQHY---- 60
           MAKFSV GEEDGED G+ TS+PRAKRKR T+  IN     PR  +RQ    + Q +    
Sbjct: 1   MAKFSVGGEEDGED-GSSTSSPRAKRKR-TSFRINAVVNFPRH-NRQREEGEEQGFDYCK 60

Query: 61  ------QTNRRRH-----DEPLPEEDSETPIWSPKSRAKNVKANSSVLMFLIDPEVFDCA 120
                 +  R+RH     +E + E+ +E  + S KS +KNVK N S+ MFLIDPEV DC+
Sbjct: 61  EETASDEEQRKRHRIDEIEEQVGEDGTEKALCSVKSCSKNVKENGSISMFLIDPEVLDCS 120

Query: 121 ICCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVLESIKLPCKQS 180
           ICCEPL++PIFQCENGHIACSSCC K+KN+CATCSWPIGYNRCRAVEKVLESI+LPCKQS
Sbjct: 121 ICCEPLSVPIFQCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLESIRLPCKQS 180

Query: 181 KYGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHTDSATNFQFSS 240
           KYGCKEIV YG+ESDH++ CL+ PCKCPLSDCNF+ASSKQLSLHFSS+HTDSA +FQFSS
Sbjct: 181 KYGCKEIVSYGSESDHDKKCLHAPCKCPLSDCNFVASSKQLSLHFSSKHTDSAISFQFSS 240

Query: 241 CFNIICLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPTSKEAFSYSIRVET 300
            F  ICL  +D+Y VLQEQ DGILFILS+S++  GNAVK+CC+RPP+ KEAFSY +RVET
Sbjct: 241 SFT-ICLNANDNYCVLQEQGDGILFILSNSFDHLGNAVKVCCIRPPSLKEAFSYDLRVET 300

Query: 301 QDLSLVLQSSMKNIRSSSDFCRSTGFLLIPRDVFGTSGKVKLNVCI-QKNGSSP 339
           QDLSLVLQSSMKNIRSSSD   ST FLLIPRD+F +S K+KLN+CI Q  G +P
Sbjct: 301 QDLSLVLQSSMKNIRSSSDCYPSTRFLLIPRDLFSSSSKIKLNICIWQNTGGAP 350

BLAST of Sed0008308 vs. ExPASy Swiss-Prot
Match: Q84K34 (E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g37930 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.9e-51
Identity = 119/344 (34.59%), Postives = 186/344 (54.07%), Query Frame = 0

Query: 1   MAKFSVDGEEDGEDGGAGTSNPRAKRKRTTTVPIN------PGFPPPREPDRQETANDHQ 60
           MA+FSV G +DGE G +  ++   KR+R  ++  N             E D  ET N   
Sbjct: 1   MARFSVCGGDDGE-GPSNNNHQSRKRQRLPSIDENEEDAETSDAGSSGEEDEDETQNQGM 60

Query: 61  HYQTNRRRHDEPLPEEDSETPIWSPK------SRAKNVKANSSVLMFLIDPEVFDCAICC 120
             ++  R       + D E  I   +      S++ +   +S + + L+DP+V DC ICC
Sbjct: 61  RPESEDR--GSTSDDSDREVVIEERRFGKFVNSQSSSSSKDSPLSVTLLDPDVLDCPICC 120

Query: 121 EPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYG 180
           EPL +PIFQC+NGH+AC+ CC K++N+C +C+ PIGY RCRA+EKV+E+ ++ C  +KYG
Sbjct: 121 EPLKIPIFQCDNGHLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNAKYG 180

Query: 181 CKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHTDSATNFQFSSCFN 240
           CKE   YGN   HE+ C++TPC CP+ DC++    K L+ H  +EH D   +F +++   
Sbjct: 181 CKESTSYGNRFSHEQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNTRLT 240

Query: 241 IICLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPT-SKEAFSYSIRVETQD 300
           I   +N  +  +LQE++DG + ++     L    V + C+ P T      S  +   T D
Sbjct: 241 ISLDLNKKT-TILQEENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGRLSCRLAKITVD 300

Query: 301 LSLVLQSSMKNIRSSSDFCRSTGFLLIPRDVFGTSGKVKLNVCI 332
             L     +KNI+  ++     GF+LIP  +F  +  + L + I
Sbjct: 301 SLLKQGFMVKNIQKVTNEHPEDGFMLIPSYLFSGNDNLNLQIWI 340

BLAST of Sed0008308 vs. ExPASy Swiss-Prot
Match: Q9FKD7 (E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g37890 PE=2 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 3.3e-40
Identity = 89/244 (36.48%), Postives = 134/244 (54.92%), Query Frame = 0

Query: 78  SPKSRAKNVKANSSVLMFLIDPEVFDCAICCEPLTLPIFQCENGHIACSSCCIKIKNKCA 137
           S  +  ++ K  S++LM   D E+ DC IC E  T+PIFQC+NGH+ACSSCC K+ NKC 
Sbjct: 28  SSDAAKRDAKKRSTMLM---DLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNNKCP 87

Query: 138 TCSWPIGYNRCRAVEKVLESIKLPCKQSKYGCKEIVGYGNESDHEETCLYTPCKCPLSDC 197
            C+ P+G+NRCRA+E VLESI +PC  +K GCK+ V YG E  HE+ C+++ C CP  DC
Sbjct: 88  ACTSPVGHNRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPALDC 147

Query: 198 NFMASSKQLSLHFSSEHTDSATNFQFSSCFNIICLINDDSYDVLQ-EQDDGILFILSHSY 257
           N+ +S K L  H+   H +      F     +   +N     +++ E     LF +    
Sbjct: 148 NYTSSYKDLYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQCFR 207

Query: 258 ELQGNAVKICCLRPPTSK-EAFSYSIRVETQDLSLVLQS-SMKNIRSSSDFCRSTGFLLI 317
           E  G  V + C+ P + +   +SY++       +++ QS  +K +   S       F+LI
Sbjct: 208 EPYGVYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSFQTPQENFMLI 267

Query: 318 PRDV 319
           P  +
Sbjct: 268 PNSL 268

BLAST of Sed0008308 vs. ExPASy Swiss-Prot
Match: Q9FKD9 (Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702 GN=At5g37870 PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 9.7e-40
Identity = 81/227 (35.68%), Postives = 130/227 (57.27%), Query Frame = 0

Query: 96  LIDPEVFDCAICCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVL 155
           L D ++ DC IC + L +P+FQC NGH+ACSSCC K++NKC  C+ P+G+ RCRA+E+VL
Sbjct: 37  LTDLDILDCPICYQALKIPVFQCGNGHLACSSCCPKLRNKCPACALPVGHIRCRAMERVL 96

Query: 156 ESIKLPCKQSKYGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHT 215
           ES+ +PC+ +  GC + + YG ES HE+ C ++PC CP+  CN+  S K L  H+   H+
Sbjct: 97  ESVLVPCRYADLGCTKTIYYGRESTHEKICNFSPCSCPVQGCNYTGSYKDLYEHYDLTHS 156

Query: 216 DSATNFQFSSCFNIICLI-NDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPTSK 275
             +T + F+    I  ++   D   + +  +  +LF++    E  G  V + C+ P   +
Sbjct: 157 TGSTAYSFNGVSYIAAMMFISDKILIERVYEKKLLFVVQCFEEPCGVYVSVSCIAPSAPE 216

Query: 276 EA-FSYSIRVET-QDLSLVLQS-SMKNIRSSSDFCRSTGFLLIPRDV 319
              FSY +   T + +++  QS  +K +   S       F+LIP  +
Sbjct: 217 VGEFSYGLLYTTWEGVTMTYQSPKVKKVLKVSSQRPKDSFMLIPHSL 263

BLAST of Sed0008308 vs. ExPASy Swiss-Prot
Match: Q9FKD5 (Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702 GN=At5g37910 PE=3 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.5e-36
Identity = 80/228 (35.09%), Postives = 122/228 (53.51%), Query Frame = 0

Query: 96  LIDPEVFDCAICCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVL 155
           L+D ++ DC ICCE LT PIFQC+NGH+AC SCC K+ NKC  C+ P+G++R RA+E VL
Sbjct: 30  LLDLDILDCPICCEALTSPIFQCDNGHLACGSCCPKLSNKCPACTLPVGHSRSRAMESVL 89

Query: 156 ESIKLPCKQSKYGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEH- 215
           ESI +PC   ++GC +   YG ES HE+ C+++ C CP S C++  S K L  H+   H 
Sbjct: 90  ESILIPCPNVRFGCTKSFFYGKESAHEKECIFSQCSCPSSVCDYTGSYKDLYAHYKLTHS 149

Query: 216 TDSATNFQFSSCFNII--CLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPT 275
           T+   N +   C N     ++  D   + +  +  +L  +    E  G  V +  + P  
Sbjct: 150 TNIFWNIKRFRCANFFTTSMLISDKILIKRVHEKKLLLAVQCFREPCGVYVTVSFIAPSA 209

Query: 276 SKEA-FSYSIRVETQDLSLVLQS-SMKNIRSSSDFCRSTGFLLIPRDV 319
            +   FSY +       ++  +S  +K +   S       F+LIP  +
Sbjct: 210 PEVGEFSYQLSYNVDGHTVTYESPEVKRVCKVSIETPQENFMLIPHSL 257

BLAST of Sed0008308 vs. ExPASy Swiss-Prot
Match: Q9C6H3 (E3 ubiquitin-protein ligase SINA-like 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66620 PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.2e-35
Identity = 83/244 (34.02%), Postives = 120/244 (49.18%), Query Frame = 0

Query: 96  LIDPEVFDCAICCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVL 155
           L + ++ DC ICC  LT PIFQC+NGHIACSSCC K++NKC +C+ PIG  R R +E+V+
Sbjct: 36  LFELDLLDCPICCHALTSPIFQCDNGHIACSSCCTKLRNKCPSCALPIGNFRSRIMERVV 95

Query: 156 ESIKLPCKQSKYGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHT 215
           E++ + C   K+GC E   YG E  HE+ C +  C CP  +CN+    K L  HF   H 
Sbjct: 96  EAVMVTCPNVKHGCTEKFSYGKELIHEKDCRFALCYCPAPNCNYSGVYKDLYSHFYVNHY 155

Query: 216 DSATNFQFSSCFNII-CLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRP--PT 275
           D+        C N     +      ++ +   G L  +    E QG  V + C+ P  P 
Sbjct: 156 DTWNQI---GCGNFAGAWLRISEKILVLQYGQGPLIAVQCFKETQGMYVTVNCIAPCAPG 215

Query: 276 SKE---AFSYSIRVETQDLSLVLQSSMKNIRSSSDFCRSTGFLLIPRDVFGTSGKVKLNV 334
             E     SY + +      +     M  I+  S       F+L+P    G    +K+ +
Sbjct: 216 VGELSFELSYKMPMGGNSTMMFKSEEMNRIQKVSFQTPEKDFMLVPYYFLGDFSTLKMEI 275

BLAST of Sed0008308 vs. ExPASy TrEMBL
Match: A0A6J1GYB0 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111458302 PE=3 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 9.2e-126
Identity = 239/356 (67.13%), Postives = 281/356 (78.93%), Query Frame = 0

Query: 1   MAKFSVDGEEDGEDGGAGTSNPRAKRKRTTTVPINPGFPPPREPDR-------------Q 60
           MAKFSV GEEDGED G+ TS+PRAKRKR T+  IN     PR   R             +
Sbjct: 1   MAKFSVGGEEDGED-GSSTSSPRAKRKR-TSFRINAVVNFPRHNQRCEEGEEQGFDYCKE 60

Query: 61  ETANDHQHYQTNRRRH-----DEPLPEEDSETPIWSPKSRAKNVKANSSVLMFLIDPEVF 120
           ETA+D +     R+RH     +E + E+ +E  + S KS ++NVK N S+ MFLIDPEV 
Sbjct: 61  ETASDEE----QRKRHRIDGIEEQVEEDGTENALCSVKSCSRNVKENGSISMFLIDPEVL 120

Query: 121 DCAICCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVLESIKLPC 180
           DC+ICCEPL++PIFQCENGHIACSSCC K+KN+CATCSWPIGYNRCRAVEKVLESIKLPC
Sbjct: 121 DCSICCEPLSVPIFQCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLESIKLPC 180

Query: 181 KQSKYGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHTDSATNFQ 240
           KQSKYGCKE+V YG+ESDHE+ CL+ PCKCPL DCNF+ASSKQLSLHFSS+HTDSA +FQ
Sbjct: 181 KQSKYGCKEMVSYGSESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFSSKHTDSAISFQ 240

Query: 241 FSSCFNIICLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPTSKEAFSYSIR 300
           FSS F  ICL  +D+Y VLQEQ DGILFILS+S++  GNAVK+CCLRPP+ KEAFSY +R
Sbjct: 241 FSSSFT-ICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPSLKEAFSYDLR 300

Query: 301 VETQDLSLVLQSSMKNIRSSSDFCRSTGFLLIPRDVFGTSGKVKLNVCIQKNGSSP 339
           VETQDLSLVLQSSMKNI+SSSD C ST FLLIPRD+F +SG +KLN+CI +N  +P
Sbjct: 301 VETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICIWQNTGAP 349

BLAST of Sed0008308 vs. ExPASy TrEMBL
Match: A0A1S3CD49 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499310 PE=3 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.7e-124
Identity = 236/357 (66.11%), Postives = 275/357 (77.03%), Query Frame = 0

Query: 1   MAKFSVDGEEDGEDGGAGTSNPRAKRKRTT-TVPINPGFPPPRE-----------PDRQE 60
           MAKFSVDGE   ED G G S+PR KRKR++  +     FP   E            D++E
Sbjct: 1   MAKFSVDGE---EDAGDGPSSPRGKRKRSSFRINATVDFPRRSEQREQGEEQGFNSDKEE 60

Query: 61  TANDHQHYQTNRRRHD---EPLPEEDSETPIWSPKSRAKNVKANSSVLMFLIDPEVFDCA 120
           + ND    Q+ R R+D   E L E+  +  +WS KS  KN K N SV MFLIDPEV DC+
Sbjct: 61  SENDED--QSKRHRNDEIEEHLREDGGKRALWSGKSCTKNAKPNGSVSMFLIDPEVLDCS 120

Query: 121 ICCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVLESIKLPCKQS 180
           ICCEPL++PIFQCENGHIACSSCC K+KNKCATCSWPIGYNRCRAVEKVLESIKLPCKQS
Sbjct: 121 ICCEPLSIPIFQCENGHIACSSCCNKLKNKCATCSWPIGYNRCRAVEKVLESIKLPCKQS 180

Query: 181 KYGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHTDSATNFQFSS 240
           K+GCKEI+GYGNESDHE+TCLY PC CP SDC F+AS+KQLSLHFS++HT+SATNF FSS
Sbjct: 181 KFGCKEIIGYGNESDHEKTCLYAPCLCPYSDCKFIASAKQLSLHFSNKHTNSATNFHFSS 240

Query: 241 CFNIICLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPTSKEAFSYSIRVET 300
            F  ICL  +D+Y VLQEQ DG+LFILS+S E  G+ VKICCL+PP+ KE FSY +R ET
Sbjct: 241 SFT-ICLKTNDTYHVLQEQ-DGVLFILSNSSENLGSVVKICCLQPPSLKETFSYDLRAET 300

Query: 301 QDLSLVLQSSMKNIRSSSDFCRSTGFLLIPRDVFGTSGKVKLNVCIQKNGSSPVSDR 343
           QDLSLVLQSSMKNI+SSSD C ST FL+IPRD+F +SG +KLN+CI +NGSSPV  R
Sbjct: 301 QDLSLVLQSSMKNIQSSSDCCHSTRFLMIPRDLFSSSGMIKLNICIWQNGSSPVHHR 350

BLAST of Sed0008308 vs. ExPASy TrEMBL
Match: A0A6J1JTG5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111487698 PE=3 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 5.0e-124
Identity = 238/354 (67.23%), Postives = 280/354 (79.10%), Query Frame = 0

Query: 1   MAKFSVDGEEDGEDGGAGTSNPRAKRKRTTTVPINPGFPPPREPDRQETANDHQHY---- 60
           MAKFSV GEEDGED G+ TS+PRAKRKR T+  IN     PR  +RQ    + Q +    
Sbjct: 1   MAKFSVGGEEDGED-GSSTSSPRAKRKR-TSFRINAVVNFPRH-NRQREEGEEQGFDYCK 60

Query: 61  ------QTNRRRH-----DEPLPEEDSETPIWSPKSRAKNVKANSSVLMFLIDPEVFDCA 120
                 +  R+RH     +E + E+ +E  + S KS +KNVK N S+ MFLIDPEV DC+
Sbjct: 61  EETASDEEQRKRHRIDEIEEQVGEDGTEKALCSVKSCSKNVKENGSISMFLIDPEVLDCS 120

Query: 121 ICCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVLESIKLPCKQS 180
           ICCEPL++PIFQCENGHIACSSCC K+KN+CATCSWPIGYNRCRAVEKVLESI+LPCKQS
Sbjct: 121 ICCEPLSVPIFQCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLESIRLPCKQS 180

Query: 181 KYGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHTDSATNFQFSS 240
           KYGCKEIV YG+ESDH++ CL+ PCKCPLSDCNF+ASSKQLSLHFSS+HTDSA +FQFSS
Sbjct: 181 KYGCKEIVSYGSESDHDKKCLHAPCKCPLSDCNFVASSKQLSLHFSSKHTDSAISFQFSS 240

Query: 241 CFNIICLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPTSKEAFSYSIRVET 300
            F  ICL  +D+Y VLQEQ DGILFILS+S++  GNAVK+CC+RPP+ KEAFSY +RVET
Sbjct: 241 SFT-ICLNANDNYCVLQEQGDGILFILSNSFDHLGNAVKVCCIRPPSLKEAFSYDLRVET 300

Query: 301 QDLSLVLQSSMKNIRSSSDFCRSTGFLLIPRDVFGTSGKVKLNVCI-QKNGSSP 339
           QDLSLVLQSSMKNIRSSSD   ST FLLIPRD+F +S K+KLN+CI Q  G +P
Sbjct: 301 QDLSLVLQSSMKNIRSSSDCYPSTRFLLIPRDLFSSSSKIKLNICIWQNTGGAP 350

BLAST of Sed0008308 vs. ExPASy TrEMBL
Match: A0A6J1CL96 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111011997 PE=3 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.8e-121
Identity = 226/350 (64.57%), Postives = 273/350 (78.00%), Query Frame = 0

Query: 1   MAKFSVDGEEDGEDGGAGTSNPRAKRKR-TTTVPINPGFPPPREP------DRQETANDH 60
           MAKFSV GE+DGEDG  G S+P  KR+R ++ +     +P   EP      D+++T  + 
Sbjct: 29  MAKFSVGGEDDGEDG--GPSSPTGKRRRPSSRINATVDYPHQNEPREAFDSDKEQTETEE 88

Query: 61  QHYQTNRRRHD--EPLPEEDSETPIWSPKSRAKNVKAN--SSVLMFLIDPEVFDCAICCE 120
              +   R H+  + +PE  ++  +WS  S +K +K N   SV MFLIDPEV DC+ICCE
Sbjct: 89  LDQRKRHRIHEIHQQVPEGATKKALWSVTSCSKTLKTNGGGSVPMFLIDPEVLDCSICCE 148

Query: 121 PLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGC 180
           PLT+PIFQCENGHIACSSCC K+KNKCA+CSWPIGYNRCRAVEKVLESIKLPCKQS+YGC
Sbjct: 149 PLTVPIFQCENGHIACSSCCNKLKNKCASCSWPIGYNRCRAVEKVLESIKLPCKQSQYGC 208

Query: 181 KEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHTDSATNFQFSSCFNI 240
           KE+V YGNES+HE+TCLY PC CPLSDCNF ASSKQLSLHFSS+H +SATNFQFSS F  
Sbjct: 209 KEMVSYGNESEHEKTCLYAPCLCPLSDCNFTASSKQLSLHFSSKHKNSATNFQFSSSFT- 268

Query: 241 ICLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPTSKEAFSYSIRVETQDLS 300
           ICL  DDSY +LQEQDDGILFILS+S+E  G+AVK+CCLRP   KEAF+Y ++ ETQDLS
Sbjct: 269 ICLKTDDSYHILQEQDDGILFILSNSFEHLGSAVKVCCLRPNFLKEAFTYDLKAETQDLS 328

Query: 301 LVLQSSMKNIRSSSDFCRSTGFLLIPRDVFGTSGKVKLNVCIQKNGSSPV 340
           L L SSMKNI+SSSD C ST FLLIP D+F + G++KL++CI ++GSSPV
Sbjct: 329 LFLHSSMKNIKSSSDCCHSTRFLLIPSDLFDSRGQIKLDICIWRDGSSPV 375

BLAST of Sed0008308 vs. ExPASy TrEMBL
Match: A0A2R6RKR1 (RING-type E3 ubiquitin transferase OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc05900 PE=3 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 1.4e-73
Identity = 151/348 (43.39%), Postives = 209/348 (60.06%), Query Frame = 0

Query: 1   MAKFSVDGEEDGEDGGAGTSNPRAKRKRTTTVPINPGFPPPREPDRQETANDHQHYQTNR 60
           MAKFSV  E+D E    G S+PR K++RTTT          +  + +E   + +H     
Sbjct: 1   MAKFSVGREDDSE----GPSSPRPKKQRTTTNQQQQQQQQQQVVEDEEEVVESEHGAEEE 60

Query: 61  RRHDEPLPEEDSET-----------------PIWSPKSRAKNVKANSSVLMFLIDPEVFD 120
            R  E   EE+SE                  P WS   R      + S+ + L DP+V D
Sbjct: 61  ERESEYDEEEESEEEEDDEEDDQNETETIAGPSWSKDDR------DGSISVTLTDPDVLD 120

Query: 121 CAICCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVLESIKLPCK 180
           C IC EPL+ P+FQCENGH+ACSSCCIK++NKC +CSWPIGYNRCRA+EKV+ES+K+ C+
Sbjct: 121 CPICLEPLSSPVFQCENGHVACSSCCIKLRNKCPSCSWPIGYNRCRAIEKVIESVKISCE 180

Query: 181 QSKYGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHTDSATNFQF 240
             KYGCKE V Y  +  HE+ C + PC CPLS C F+ SS QLSLHFSS H +S  +F++
Sbjct: 181 NLKYGCKETVIYSKKHGHEKACTFAPCSCPLSACTFLGSSNQLSLHFSSTHPNSGKHFRY 240

Query: 241 SSCFNIICLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPTSKEAFSYSIRV 300
           +  F I   +      +L+EQ +G +F+L++++E+ GNAV I C+ P +SK +FSY +  
Sbjct: 241 NCLFPINLEMRQKCL-ILKEQSEGKIFVLNNAFEIMGNAVNISCIGPSSSKSSFSYDLIA 300

Query: 301 ETQDLSLVLQSSMKNIRSSSDFCRSTGFLLIPRDVFGTSGKVKLNVCI 332
                SL LQS  KN+    +   +  FLL+P + FG+ G++KL +CI
Sbjct: 301 RVGGSSLKLQSFTKNVPRWVEHTPAERFLLVPNNFFGSCGQLKLELCI 337

BLAST of Sed0008308 vs. TAIR 10
Match: AT5G37930.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 204.5 bits (519), Expect = 1.3e-52
Identity = 119/344 (34.59%), Postives = 186/344 (54.07%), Query Frame = 0

Query: 1   MAKFSVDGEEDGEDGGAGTSNPRAKRKRTTTVPIN------PGFPPPREPDRQETANDHQ 60
           MA+FSV G +DGE G +  ++   KR+R  ++  N             E D  ET N   
Sbjct: 1   MARFSVCGGDDGE-GPSNNNHQSRKRQRLPSIDENEEDAETSDAGSSGEEDEDETQNQGM 60

Query: 61  HYQTNRRRHDEPLPEEDSETPIWSPK------SRAKNVKANSSVLMFLIDPEVFDCAICC 120
             ++  R       + D E  I   +      S++ +   +S + + L+DP+V DC ICC
Sbjct: 61  RPESEDR--GSTSDDSDREVVIEERRFGKFVNSQSSSSSKDSPLSVTLLDPDVLDCPICC 120

Query: 121 EPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYG 180
           EPL +PIFQC+NGH+AC+ CC K++N+C +C+ PIGY RCRA+EKV+E+ ++ C  +KYG
Sbjct: 121 EPLKIPIFQCDNGHLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNAKYG 180

Query: 181 CKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHTDSATNFQFSSCFN 240
           CKE   YGN   HE+ C++TPC CP+ DC++    K L+ H  +EH D   +F +++   
Sbjct: 181 CKESTSYGNRFSHEQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNTRLT 240

Query: 241 IICLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPT-SKEAFSYSIRVETQD 300
           I   +N  +  +LQE++DG + ++     L    V + C+ P T      S  +   T D
Sbjct: 241 ISLDLNKKT-TILQEENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGRLSCRLAKITVD 300

Query: 301 LSLVLQSSMKNIRSSSDFCRSTGFLLIPRDVFGTSGKVKLNVCI 332
             L     +KNI+  ++     GF+LIP  +F  +  + L + I
Sbjct: 301 SLLKQGFMVKNIQKVTNEHPEDGFMLIPSYLFSGNDNLNLQIWI 340

BLAST of Sed0008308 vs. TAIR 10
Match: AT5G37890.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 167.2 bits (422), Expect = 2.4e-41
Identity = 89/244 (36.48%), Postives = 134/244 (54.92%), Query Frame = 0

Query: 78  SPKSRAKNVKANSSVLMFLIDPEVFDCAICCEPLTLPIFQCENGHIACSSCCIKIKNKCA 137
           S  +  ++ K  S++LM   D E+ DC IC E  T+PIFQC+NGH+ACSSCC K+ NKC 
Sbjct: 28  SSDAAKRDAKKRSTMLM---DLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNNKCP 87

Query: 138 TCSWPIGYNRCRAVEKVLESIKLPCKQSKYGCKEIVGYGNESDHEETCLYTPCKCPLSDC 197
            C+ P+G+NRCRA+E VLESI +PC  +K GCK+ V YG E  HE+ C+++ C CP  DC
Sbjct: 88  ACTSPVGHNRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPALDC 147

Query: 198 NFMASSKQLSLHFSSEHTDSATNFQFSSCFNIICLINDDSYDVLQ-EQDDGILFILSHSY 257
           N+ +S K L  H+   H +      F     +   +N     +++ E     LF +    
Sbjct: 148 NYTSSYKDLYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQCFR 207

Query: 258 ELQGNAVKICCLRPPTSK-EAFSYSIRVETQDLSLVLQS-SMKNIRSSSDFCRSTGFLLI 317
           E  G  V + C+ P + +   +SY++       +++ QS  +K +   S       F+LI
Sbjct: 208 EPYGVYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSFQTPQENFMLI 267

Query: 318 PRDV 319
           P  +
Sbjct: 268 PNSL 268

BLAST of Sed0008308 vs. TAIR 10
Match: AT5G37870.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 165.6 bits (418), Expect = 6.9e-41
Identity = 81/227 (35.68%), Postives = 130/227 (57.27%), Query Frame = 0

Query: 96  LIDPEVFDCAICCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVL 155
           L D ++ DC IC + L +P+FQC NGH+ACSSCC K++NKC  C+ P+G+ RCRA+E+VL
Sbjct: 37  LTDLDILDCPICYQALKIPVFQCGNGHLACSSCCPKLRNKCPACALPVGHIRCRAMERVL 96

Query: 156 ESIKLPCKQSKYGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHT 215
           ES+ +PC+ +  GC + + YG ES HE+ C ++PC CP+  CN+  S K L  H+   H+
Sbjct: 97  ESVLVPCRYADLGCTKTIYYGRESTHEKICNFSPCSCPVQGCNYTGSYKDLYEHYDLTHS 156

Query: 216 DSATNFQFSSCFNIICLI-NDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPTSK 275
             +T + F+    I  ++   D   + +  +  +LF++    E  G  V + C+ P   +
Sbjct: 157 TGSTAYSFNGVSYIAAMMFISDKILIERVYEKKLLFVVQCFEEPCGVYVSVSCIAPSAPE 216

Query: 276 EA-FSYSIRVET-QDLSLVLQS-SMKNIRSSSDFCRSTGFLLIPRDV 319
              FSY +   T + +++  QS  +K +   S       F+LIP  +
Sbjct: 217 VGEFSYGLLYTTWEGVTMTYQSPKVKKVLKVSSQRPKDSFMLIPHSL 263

BLAST of Sed0008308 vs. TAIR 10
Match: AT5G37910.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 152.9 bits (385), Expect = 4.6e-37
Identity = 80/228 (35.09%), Postives = 122/228 (53.51%), Query Frame = 0

Query: 96  LIDPEVFDCAICCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVL 155
           L+D ++ DC ICCE LT PIFQC+NGH+AC SCC K+ NKC  C+ P+G++R RA+E VL
Sbjct: 30  LLDLDILDCPICCEALTSPIFQCDNGHLACGSCCPKLSNKCPACTLPVGHSRSRAMESVL 89

Query: 156 ESIKLPCKQSKYGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEH- 215
           ESI +PC   ++GC +   YG ES HE+ C+++ C CP S C++  S K L  H+   H 
Sbjct: 90  ESILIPCPNVRFGCTKSFFYGKESAHEKECIFSQCSCPSSVCDYTGSYKDLYAHYKLTHS 149

Query: 216 TDSATNFQFSSCFNII--CLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRPPT 275
           T+   N +   C N     ++  D   + +  +  +L  +    E  G  V +  + P  
Sbjct: 150 TNIFWNIKRFRCANFFTTSMLISDKILIKRVHEKKLLLAVQCFREPCGVYVTVSFIAPSA 209

Query: 276 SKEA-FSYSIRVETQDLSLVLQS-SMKNIRSSSDFCRSTGFLLIPRDV 319
            +   FSY +       ++  +S  +K +   S       F+LIP  +
Sbjct: 210 PEVGEFSYQLSYNVDGHTVTYESPEVKRVCKVSIETPQENFMLIPHSL 257

BLAST of Sed0008308 vs. TAIR 10
Match: AT1G66620.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 150.6 bits (379), Expect = 2.3e-36
Identity = 83/244 (34.02%), Postives = 120/244 (49.18%), Query Frame = 0

Query: 96  LIDPEVFDCAICCEPLTLPIFQCENGHIACSSCCIKIKNKCATCSWPIGYNRCRAVEKVL 155
           L + ++ DC ICC  LT PIFQC+NGHIACSSCC K++NKC +C+ PIG  R R +E+V+
Sbjct: 36  LFELDLLDCPICCHALTSPIFQCDNGHIACSSCCTKLRNKCPSCALPIGNFRSRIMERVV 95

Query: 156 ESIKLPCKQSKYGCKEIVGYGNESDHEETCLYTPCKCPLSDCNFMASSKQLSLHFSSEHT 215
           E++ + C   K+GC E   YG E  HE+ C +  C CP  +CN+    K L  HF   H 
Sbjct: 96  EAVMVTCPNVKHGCTEKFSYGKELIHEKDCRFALCYCPAPNCNYSGVYKDLYSHFYVNHY 155

Query: 216 DSATNFQFSSCFNII-CLINDDSYDVLQEQDDGILFILSHSYELQGNAVKICCLRP--PT 275
           D+        C N     +      ++ +   G L  +    E QG  V + C+ P  P 
Sbjct: 156 DTWNQI---GCGNFAGAWLRISEKILVLQYGQGPLIAVQCFKETQGMYVTVNCIAPCAPG 215

Query: 276 SKE---AFSYSIRVETQDLSLVLQSSMKNIRSSSDFCRSTGFLLIPRDVFGTSGKVKLNV 334
             E     SY + +      +     M  I+  S       F+L+P    G    +K+ +
Sbjct: 216 VGELSFELSYKMPMGGNSTMMFKSEEMNRIQKVSFQTPEKDFMLVPYYFLGDFSTLKMEI 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023534163.13.5e-12768.26E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita pepo subsp. pepo][more]
XP_022956630.11.9e-12567.13E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita moschata] >KAG6602037.1 E3 u... [more]
XP_038874338.15.5e-12566.57putative E3 ubiquitin-protein ligase SINA-like 6 [Benincasa hispida][more]
XP_008460511.13.6e-12466.11PREDICTED: E3 ubiquitin-protein ligase SINA-like 7 [Cucumis melo][more]
XP_022990964.11.0e-12367.23E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q84K341.9e-5134.59E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g... [more]
Q9FKD73.3e-4036.48E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g3... [more]
Q9FKD99.7e-4035.68Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702... [more]
Q9FKD56.5e-3635.09Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702... [more]
Q9C6H33.2e-3534.02E3 ubiquitin-protein ligase SINA-like 2 OS=Arabidopsis thaliana OX=3702 GN=At1g6... [more]
Match NameE-valueIdentityDescription
A0A6J1GYB09.2e-12667.13RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111458302... [more]
A0A1S3CD491.7e-12466.11RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499310 PE=3 ... [more]
A0A6J1JTG55.0e-12467.23RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111487698 P... [more]
A0A6J1CL961.8e-12164.57RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101199... [more]
A0A2R6RKR11.4e-7343.39RING-type E3 ubiquitin transferase OS=Actinidia chinensis var. chinensis OX=1590... [more]
Match NameE-valueIdentityDescription
AT5G37930.11.3e-5234.59Protein with RING/U-box and TRAF-like domains [more]
AT5G37890.12.4e-4136.48Protein with RING/U-box and TRAF-like domains [more]
AT5G37870.16.9e-4135.68Protein with RING/U-box and TRAF-like domains [more]
AT5G37910.14.6e-3735.09Protein with RING/U-box and TRAF-like domains [more]
AT1G66620.12.3e-3634.02Protein with RING/U-box and TRAF-like domains [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 147..316
e-value: 2.0E-20
score: 73.6
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 152..210
e-value: 2.8E-15
score: 58.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..70
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..78
NoneNo IPR availablePANTHERPTHR46632:SF16E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4coord: 46..335
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 157..332
IPR044286E3 ubiquitin-protein ligase SINA-like, plantPANTHERPTHR46632E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4coord: 46..335
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 157..215
score: 14.168446

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0008308.1Sed0008308.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016740 transferase activity
molecular_function GO:0008270 zinc ion binding