Sed0008012 (gene) Chayote v1

Overview
NameSed0008012
Typegene
OrganismSechium edule (Chayote v1)
DescriptionZinc ion binding,DNA binding, putative isoform 3
LocationLG07: 22366139 .. 22370622 (+)
RNA-Seq ExpressionSed0008012
SyntenySed0008012
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAAAAGACTTGTACTTCAAAAGTCGTCAGAACAAGCACGGTTGATACATGAGCTTCCAGAAGTGTTACCAGATATTCCTGAACCTACGTACGATGATTTACTAAAGAATGCTGAAGAAGAAAATTACATGTTGGTTGATGGGAGAGACGACACAAAAGTTGCCACAGGTTAGTTATCTCTGTAGTAGATACGTTTTTTTTTTCTTGGTCAAGCTTTGCTCTTTTCTTACAACTTTTTGAATGAGGGATGTTTGGATGTTCTCCTGTACGTTTGAAAAGTGCTCAGTTTTGGACGGTTCTTCGATCATAGTTGCTATATTGAATTTACGAGCCATTATCTATTAGATTATGGTTAAGATATTGTAAATATCGTGATTTAGTTTTGTAAGTTGTTTAGATTTGCACTCTAAGTTTGTATATATATATATATATTCCCTTGTTCACTTGGGAAGATAACTAAGAAAATGAGATTTCTCTATTCTCTGATCTATGATTTAATATAGTGTCAGAGCTCATGATTGGAGCTATTTAGGGTTTGTCAAAATATCTTCATTCCATTTTCTGATTTCACTTTTTTGTTTTTTTTTTGAAAATTTCTGATTTCAGTTGCCTTTTTCAATACCCATTTATAATCTTGCCTTCCCCAATATTATTTCCAAATTCATTTATGCTTCCTTTCATCCAGATTCAACATTAATGGCTGCCAAATTCAATTGCCATATTTCTTTCTATTTGCAATTGCTTCTATAAAGAAAGTTTTCCAATTGAAATTTTTGGCTTAAAAAAAAATTCATGCAGTTGTTGCAGCATCACCATTCCCAAGTTATTTGGAATTCCATGAAGCTTTTTTAGTCTGCGTCAGAGGTTGGCCCTGTTCATCAAATTGTTGCAGGCCCCTGCCTTTTTTAGACGATTCCATCAATTAGTTTTCAAATCTCAGCCATAAGTACAGGCTGCTTTTTATTCTTCCGCATCCAGCTTCATCATTTTGTCCAAAATTATTATTATTATTATTTTGGTTGCTGTGTGGTTGTTTCTACAAACAACACCGTCAATCAAAGTTGTTTTATTCTGCTGCTATGATTCGTGTTGTTGAATCAGCAGAGTTTTGTTCTTCAGTTGCTATTGTGGATAATTTTCAGAACAAGTTTATGTTGCCTAATCATAGTGCAACACGTTCAGCACACTCCCTCTCTCACTCGATAGGTATGCTAAATCTTCAATGTCAATAGATTCTCGATTCAGGTGCTTCCACTCATATTTGTTATTTAAAGCACATTTTCTCTAGTATGAATCCTATTGTAAGTAGTGTGATTTTGTCAAATAAGAAGAGGATTAGTGTGCACCATGAGGGTGTGGGCGACGTGGGCTGGCACGTGAGCATGCGTGGTGCGCCCGCGGGGCTAGGCAGGGACGGACGTGGCTTGGGTCGCGGGTAGGCAGGGTCGAGGTGGGCTGGTCGGACCGAGCCGGTCTGGGCCAGTCGAGTTGAACTGGAGAGAGGAGTTATGTGGGAATGTGAACGGGGAAAACCGACTGGGACCAGAATTCGATCATCAGGGAAATTCTAGGTTTGACAGGAGGGGCGACGAGTTACTAGAAGAGATGAGGGTGCGCATCGGCTATAACAGGCCATTAGCGCATGACGGTTGGAATGATCGGGGCCGAAATTGTGTTGATGATTTTTCGGGACTGGAGTTCGATCAAAGGGGAGATCCGAGGATCGTGGGGAGAGAAAACGAGGTTCTGGAGGAGAGGAGGTTGCACATGGGCTATGATAGGCCGTTGGCGCATGACGGTTGGAGCGATCGTTGGTGGGGTGACAATGTCCGAAATAGGGGACGAGATTTCGGGTGGGGAGGTGAAGAAAGATTTGAACGGGGAGGTCAGAAAGTAGCAGGAAGAGAGGGACCGGGTTGTGAAAGGAGATTTAGAAAATTGGAGACGCCGGTTTTCAAGGGTCTTGCTGATGAGGACCCAGTAGGTTGGTTATGCCGGGTCGAGCGTTATTTTCTAATAAACAAGCTGACTGAATATGAAGGGGTTGAGGCACCGGGGTTACATTTGGGAGGGGAGGCATTAGAGTGGCTTCAATATGAGGAGGACCGTGCTCCTTTTCGTTCCTGGAACGAATTCAAAGACGGGTTGGTGGAGCGTTTCCAATCGACGGCTCAGGCAAACAAGTATACCAACTTCATGAGTTTGAAGCAAATAGGCACAGTGAAGGAATATTGCCGGAGCTTTGAGCGCTTTGCTAAAGGAATGCACGATATCAGTGCGTGTGCATTGGAGGGGAAGTGGAAAAGTGGACTGAAAGAGGAGATACAGAGCGCGATGCGGAAACTGCGACCAGTGGGCATCGATGAGAAAATGTTTATGGACCAAGTGATCGAGGATGATTTTGCTTTTCAGGCTCCTAGAAATGAGGGAAGTACTTCTACAACGGTCAAGGCTAAAGCAGGAACGATTGTGTCGGGAATTGGAAGCACGGAGACTTTTTCCCACAAACTACAACCGTTCAACCGACGTCCTATAAAAAACTCATTGAAGCCGAAATTCGAGCAAGGAAGGAAGGGTTATGCTTTCACTGTGATGAACGATTTGTACCGGGGCATCGTGTCAGAAAAAGGAGCTCCAGAACTTGGATGTGTGGGTCGTTCGGGATGCGCATGATTACCCAGATGCTGATGTGACAGACTTTTCGCCAGAAGACGGAGCTGCAGAAGATGGCGCGGAGATGGAACACCTTGAGGACAAGGTGGCTCTTGGGGGGAAAGTTTTTATATGATCCAACCCTTGGAGTTTTAGAATATTGAGAATATTAGTATTTAATATTAATTGTTATTTTAACTGTATTGGGCTTGTGCCTATTATTAGTCTTTATTATTAAATTAGGTCATTATTAGGTTTACTTGGGGAGTAAGTTAGAGGCTAGGGTTTAAGGTTATAAATAGGAGATGTTAGGTCATTGTTAGGCATCCAATTGAATTGTCGTCTTTGTACTATGGCTTTTTGAGAGATCCTCTTGAGAGAATTATCGTAGTTTAATAAAAAGTGTGGTAGATTCTATCATTTTCTTGTGTGAGCAGGCCAGAACAGAATTCAGTAGTGGAAAGAAAACACCAACACATATTGAATATGGCTCGAGCACTTTTTTTTTCAATCCAAAATCCCCCTCCTCTATTGGAATGAGTTTGTCTTGACAGCTGTGCACCTGATCAATCGAACACCTTTTCCAGTGTTGGGATTGAAAACCCCTTCAAACTTCTTAAAGGAACTCAAGTAGACCGCTTAATGCTCAAAACATTTGGGTGTCTCGCATATGCTTCTACACTCTTAGGCCATAGACATAAATTTTTAGCTCGCGCTATTCCTTCAATTCTTTTGGGATATCCTTCTAGTGTGAAGTGTTTTAAGTTGCTTAGTTTAGAGACCAATGAAGTTTTTATCTCTAGAGATGTCTCTAAAGATATTGTTTTTCATGAGAATATCTATCTTTTTTTTTGTCATGGTTGTGTAGATTTTGACTACGCATGCCAGACTAAGAACGCAAAAAAATATGACTCTCTCTCCTCTCTTCCCCTATTTTCCTTTCGACCATGAATCCGACCATCTTCTTTGATGGAAGAACGCACTCTTCTACTAGGATTGTTCCTCCTGCGTAAAGCAGACATTTAATGCAAGACCTACACCCTGGATATAGATGGTAGATACAGGGGCTCCAGAGTCAAGATTATCGAATCCATGAAAGACAGAGCATTCATAATTTCCATCCACTGGTCTTCGTGGATTACATCTACATTTTCGGCCTCATTCAATGTGTCCCTCAACCACAAATTTTTCAAGGTAGAAAGAACAGATGATTTCACACTTTGGGTGGAGAAAACCAGTAATAAGAATGAATACCTGGTTGAGCTCACAAAGCTTAAACAACAATGGCAAGATCACAAAGCTAGTCATCCCAATGTGTGAGAATAGATGGGGTTGAAGCCACTTTTATACTCTGCTGCAAGGATCGACTAAACCAAAACCTCTCTCTGCAATAAAAGATCCACCTACTGTAAACCCACCATTAATCCCCATTGAGCAAAAAGGAAAACAACCTCTTTTATTACTAAACCAGGGCGGGAACAAGCCTTTATTGCAGACCAATCCTCCACTTGTTATTAATGGACCCCCTAATACACAAGCGACCCCTTACAGGTCAGCCTTACTTAAGACAAAGCAACGGAACATTCCTCGCAACCAAGAGAGAAGAACAGAGGAGAATAGACACAAAAAAGCTGAGTGGGTGATTCGATCCACAACTGCTCCAGGCAATAAGAAAGAGATTCAACCCAAGGAGGATCTGTATACCAGAAACCCATTTCCTCTCGACAATAGATTATTGTTGACTGGAATGACCACGATATTCTTCTCCAAAAAACACCTATGTACTGGAAAAACAGTGATCCCTTCTACAATGCAAAGTTTGGCCTAA

mRNA sequence

ATGGAAAAAAGACTTGTACTTCAAAAGTCGTCAGAACAAGCACGGTTGATACATGAGCTTCCAGAAGTGTTACCAGATATTCCTGAACCTACGTACGATGATTTACTAAAGAATGCTGAAGAAGAAAATTACATGTTGGTTGATGGGAGAGACGACACAAAAGTTGCCACAGATGGTAGATACAGGGGCTCCAGAGTCAAGATTATCGAATCCATGAAAGACAGAGCATTCATAATTTCCATCCACTGGTCTTCGTGGATTACATCTACATTTTCGGCCTCATTCAATGTGTCCCTCAACCACAAATTTTTCAAGGTAGAAAGAACAGATGATTTCACACTTTGGGTGGAGAAAACCAGTAATAAGAATGAATACCTGGTTGAGCTCACAAAGCTTAAACAACAATGGCAAGATCACAAAGCTAGTCATCCCAATGGCGGGAACAAGCCTTTATTGCAGACCAATCCTCCACTTGTTATTAATGGACCCCCTAATACACAAGCGACCCCTTACAGGTCAGCCTTACTTAAGACAAAGCAACGGAACATTCCTCGCAACCAAGAGAGAAGAACAGAGGAGAATAGACACAAAAAAGCTGAGTGGGTGATTCGATCCACAACTGCTCCAGGCAATAAGAAAGAGATTCAACCCAAGGAGGATCTGTATACCAGAAACCCATTTCCTCTCGACAATAGATTATTGTTGACTGGAATGACCACGATATTCTTCTCCAAAAAACACCTATGTACTGGAAAAACAGTGATCCCTTCTACAATGCAAAGTTTGGCCTAA

Coding sequence (CDS)

ATGGAAAAAAGACTTGTACTTCAAAAGTCGTCAGAACAAGCACGGTTGATACATGAGCTTCCAGAAGTGTTACCAGATATTCCTGAACCTACGTACGATGATTTACTAAAGAATGCTGAAGAAGAAAATTACATGTTGGTTGATGGGAGAGACGACACAAAAGTTGCCACAGATGGTAGATACAGGGGCTCCAGAGTCAAGATTATCGAATCCATGAAAGACAGAGCATTCATAATTTCCATCCACTGGTCTTCGTGGATTACATCTACATTTTCGGCCTCATTCAATGTGTCCCTCAACCACAAATTTTTCAAGGTAGAAAGAACAGATGATTTCACACTTTGGGTGGAGAAAACCAGTAATAAGAATGAATACCTGGTTGAGCTCACAAAGCTTAAACAACAATGGCAAGATCACAAAGCTAGTCATCCCAATGGCGGGAACAAGCCTTTATTGCAGACCAATCCTCCACTTGTTATTAATGGACCCCCTAATACACAAGCGACCCCTTACAGGTCAGCCTTACTTAAGACAAAGCAACGGAACATTCCTCGCAACCAAGAGAGAAGAACAGAGGAGAATAGACACAAAAAAGCTGAGTGGGTGATTCGATCCACAACTGCTCCAGGCAATAAGAAAGAGATTCAACCCAAGGAGGATCTGTATACCAGAAACCCATTTCCTCTCGACAATAGATTATTGTTGACTGGAATGACCACGATATTCTTCTCCAAAAAACACCTATGTACTGGAAAAACAGTGATCCCTTCTACAATGCAAAGTTTGGCCTAA

Protein sequence

MEKRLVLQKSSEQARLIHELPEVLPDIPEPTYDDLLKNAEEENYMLVDGRDDTKVATDGRYRGSRVKIIESMKDRAFIISIHWSSWITSTFSASFNVSLNHKFFKVERTDDFTLWVEKTSNKNEYLVELTKLKQQWQDHKASHPNGGNKPLLQTNPPLVINGPPNTQATPYRSALLKTKQRNIPRNQERRTEENRHKKAEWVIRSTTAPGNKKEIQPKEDLYTRNPFPLDNRLLLTGMTTIFFSKKHLCTGKTVIPSTMQSLA
Homology
BLAST of Sed0008012 vs. NCBI nr
Match: XP_022984438.1 (uncharacterized protein At5g08430-like [Cucurbita maxima])

HSP 1 Score: 93.6 bits (231), Expect = 2.7e-15
Identity = 47/57 (82.46%), Postives = 52/57 (91.23%), Query Frame = 0

Query: 1   MEKRLVLQKSSEQARLIHELPEVLPDIPEPTYDDLLKNAEEENYMLVDGRDDTKVAT 58
           MEKRL+LQKSSEQARLIHELPEV+ DI EPT+DDLLK  E+EN+MLVDGRDD KVAT
Sbjct: 515 MEKRLLLQKSSEQARLIHELPEVIADILEPTFDDLLKQNEQENHMLVDGRDDRKVAT 571

BLAST of Sed0008012 vs. NCBI nr
Match: XP_022942556.1 (uncharacterized protein At5g08430-like [Cucurbita moschata])

HSP 1 Score: 93.6 bits (231), Expect = 2.7e-15
Identity = 47/57 (82.46%), Postives = 52/57 (91.23%), Query Frame = 0

Query: 1   MEKRLVLQKSSEQARLIHELPEVLPDIPEPTYDDLLKNAEEENYMLVDGRDDTKVAT 58
           MEKRL+LQKSSEQARLIHELPEV+ DI EPT+DDLLK  E+EN+MLVDGRDD KVAT
Sbjct: 515 MEKRLLLQKSSEQARLIHELPEVIADILEPTFDDLLKQNEQENHMLVDGRDDRKVAT 571

BLAST of Sed0008012 vs. NCBI nr
Match: KAG7031352.1 (hypothetical protein SDJN02_05392, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 91.3 bits (225), Expect = 1.4e-14
Identity = 46/57 (80.70%), Postives = 51/57 (89.47%), Query Frame = 0

Query: 1   MEKRLVLQKSSEQARLIHELPEVLPDIPEPTYDDLLKNAEEENYMLVDGRDDTKVAT 58
           MEKRL+LQKSSEQARLIHELPEV+ DI EPT+DDL K  E+EN+MLVDGRDD KVAT
Sbjct: 568 MEKRLLLQKSSEQARLIHELPEVIADILEPTFDDLQKQNEQENHMLVDGRDDRKVAT 624

BLAST of Sed0008012 vs. NCBI nr
Match: XP_023539109.1 (uncharacterized protein At5g08430-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-13
Identity = 45/57 (78.95%), Postives = 50/57 (87.72%), Query Frame = 0

Query: 1   MEKRLVLQKSSEQARLIHELPEVLPDIPEPTYDDLLKNAEEENYMLVDGRDDTKVAT 58
           MEKRL+LQKSSEQARLIHELPEV+ DI EPT+DDLLK  E+EN+MLVDG D  KVAT
Sbjct: 515 MEKRLLLQKSSEQARLIHELPEVIADILEPTFDDLLKQNEQENHMLVDGSDVRKVAT 571

BLAST of Sed0008012 vs. NCBI nr
Match: KAA0060565.1 (Zinc ion binding,DNA binding, putative isoform 3 [Cucumis melo var. makuwa] >TYK02304.1 Zinc ion binding,DNA binding, putative isoform 3 [Cucumis melo var. makuwa])

HSP 1 Score: 85.5 bits (210), Expect = 7.5e-13
Identity = 41/61 (67.21%), Postives = 51/61 (83.61%), Query Frame = 0

Query: 1  MEKRLVLQKSSEQARLIHELPEVLPDIPEPTYDDLLKNAEEENYMLVDGRDDTKVATDGR 60
          MEKR++LQK SEQARLIHELP+V+PDIPEPT++DLL+  EE N++LVD  D  KVATD  
Sbjct: 1  MEKRILLQKPSEQARLIHELPKVIPDIPEPTFEDLLEEDEEVNHVLVDRSDHRKVATDAH 60

Query: 61 Y 62
          +
Sbjct: 61 F 61

BLAST of Sed0008012 vs. ExPASy TrEMBL
Match: A0A6J1JAH4 (uncharacterized protein At5g08430-like OS=Cucurbita maxima OX=3661 GN=LOC111482736 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 1.3e-15
Identity = 47/57 (82.46%), Postives = 52/57 (91.23%), Query Frame = 0

Query: 1   MEKRLVLQKSSEQARLIHELPEVLPDIPEPTYDDLLKNAEEENYMLVDGRDDTKVAT 58
           MEKRL+LQKSSEQARLIHELPEV+ DI EPT+DDLLK  E+EN+MLVDGRDD KVAT
Sbjct: 515 MEKRLLLQKSSEQARLIHELPEVIADILEPTFDDLLKQNEQENHMLVDGRDDRKVAT 571

BLAST of Sed0008012 vs. ExPASy TrEMBL
Match: A0A6J1FP67 (uncharacterized protein At5g08430-like OS=Cucurbita moschata OX=3662 GN=LOC111447554 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 1.3e-15
Identity = 47/57 (82.46%), Postives = 52/57 (91.23%), Query Frame = 0

Query: 1   MEKRLVLQKSSEQARLIHELPEVLPDIPEPTYDDLLKNAEEENYMLVDGRDDTKVAT 58
           MEKRL+LQKSSEQARLIHELPEV+ DI EPT+DDLLK  E+EN+MLVDGRDD KVAT
Sbjct: 515 MEKRLLLQKSSEQARLIHELPEVIADILEPTFDDLLKQNEQENHMLVDGRDDRKVAT 571

BLAST of Sed0008012 vs. ExPASy TrEMBL
Match: A0A5A7V3X4 (Zinc ion binding,DNA binding, putative isoform 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold18G001190 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 3.6e-13
Identity = 41/61 (67.21%), Postives = 51/61 (83.61%), Query Frame = 0

Query: 1  MEKRLVLQKSSEQARLIHELPEVLPDIPEPTYDDLLKNAEEENYMLVDGRDDTKVATDGR 60
          MEKR++LQK SEQARLIHELP+V+PDIPEPT++DLL+  EE N++LVD  D  KVATD  
Sbjct: 1  MEKRILLQKPSEQARLIHELPKVIPDIPEPTFEDLLEEDEEVNHVLVDRSDHRKVATDAH 60

Query: 61 Y 62
          +
Sbjct: 61 F 61

BLAST of Sed0008012 vs. ExPASy TrEMBL
Match: A0A1S3BTE6 (uncharacterized protein At5g08430 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493309 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 4.0e-12
Identity = 40/57 (70.18%), Postives = 49/57 (85.96%), Query Frame = 0

Query: 1   MEKRLVLQKSSEQARLIHELPEVLPDIPEPTYDDLLKNAEEENYMLVDGRDDTKVAT 58
           MEKR++LQK SEQARLIHELP+V+PDIPEPT++DLL+  EE N++LVD  D  KVAT
Sbjct: 522 MEKRILLQKPSEQARLIHELPKVIPDIPEPTFEDLLEEDEEVNHVLVDRSDHRKVAT 578

BLAST of Sed0008012 vs. ExPASy TrEMBL
Match: A0A1S3BSR8 (uncharacterized protein At5g08430 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493309 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 4.0e-12
Identity = 40/57 (70.18%), Postives = 49/57 (85.96%), Query Frame = 0

Query: 1   MEKRLVLQKSSEQARLIHELPEVLPDIPEPTYDDLLKNAEEENYMLVDGRDDTKVAT 58
           MEKR++LQK SEQARLIHELP+V+PDIPEPT++DLL+  EE N++LVD  D  KVAT
Sbjct: 522 MEKRILLQKPSEQARLIHELPKVIPDIPEPTFEDLLEEDEEVNHVLVDRSDHRKVAT 578

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022984438.12.7e-1582.46uncharacterized protein At5g08430-like [Cucurbita maxima][more]
XP_022942556.12.7e-1582.46uncharacterized protein At5g08430-like [Cucurbita moschata][more]
KAG7031352.11.4e-1480.70hypothetical protein SDJN02_05392, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023539109.11.5e-1378.95uncharacterized protein At5g08430-like [Cucurbita pepo subsp. pepo][more]
KAA0060565.17.5e-1367.21Zinc ion binding,DNA binding, putative isoform 3 [Cucumis melo var. makuwa] >TYK... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1JAH41.3e-1582.46uncharacterized protein At5g08430-like OS=Cucurbita maxima OX=3661 GN=LOC1114827... [more]
A0A6J1FP671.3e-1582.46uncharacterized protein At5g08430-like OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A5A7V3X43.6e-1367.21Zinc ion binding,DNA binding, putative isoform 3 OS=Cucumis melo var. makuwa OX=... [more]
A0A1S3BTE64.0e-1270.18uncharacterized protein At5g08430 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A1S3BSR84.0e-1270.18uncharacterized protein At5g08430 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 139..181
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 182..202
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 138..214

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0008012.1Sed0008012.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003677 DNA binding
molecular_function GO:0046872 metal ion binding