Sed0007460 (gene) Chayote v1

Overview
NameSed0007460
Typegene
OrganismSechium edule (Chayote v1)
DescriptionPyruvate kinase
LocationLG13: 2756284 .. 2761632 (+)
RNA-Seq ExpressionSed0007460
SyntenySed0007460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATTTCAAAGGAGAGAAAAGGGTTACGGCGTCAGACAACAAAGCAGCAGAAACCAGTGGCCGACACGTGACAGTTACAGGTTCAACCGAACCACGAGCCAGCGCCGGGAAAGTTAGTTGACCGGTAATTTCAAATTAAATATAAATTTAATTTCTTAATTCAAAACCTAACAGCGCAGAGCGACAAAAGCCTTTGCCATTCTCCACAGCTAGGGTTCTGCCCAATAATCTCCTCTTCGAATATTCTTCTTTTTCTTGTAAAATCCACCATTGTTCGTAGTTACTCTTTGTTCAAATCTCGGCCGACAACCCGATTCAAACCCGATCCAAGGCCGAGGTAGGCTGTTGTCGTTGTCGTTGTCGTTGCTGCTGTTGTTCGTCAATGGCGGAGGTGAATGCTATGATGATTCGTGCTCGTTGCAGTTTCGTTGCGAAAATCCCTGTACTAGATGTGAAGCGTTCGGGTTTCGGTAAGCCGGTTATTCCTCGAACGGTTCGGTGCAATCGGAGGACGAATGGGAGAGTTGGAGCTGGAGTGAAGGCAGCAATGCAGGTGGGTTTGGAGGATGAGGAGAGGTTGAAGAGATTGGAGGGTTTAGAGGCGACGTTAGGGCTCGATGTCGTGGTGGAGAGGGAGTTGAAGGAGAAAGGGTTTTTGGGGATGAGGAAGACGAAGCTTGTTTGTACGATCGGCCCTGCGTGTTGTGCGGTCGAGGATTTGGAGAAGTTGGCTTTGGAAGGGATGAACATTGCGAGGCTGAATATGTGTCATAATACGAAGGAGTGGCACTGTGATGTGATTAAGAAGATTAACAAGTTGAATGAGGAGAAAGGGTTTTGTCTTTCTGTGATGATTGACACTGAAGGAAGTCAGATTCATGTTGTTGATCATGGAGCTCCTTCCTCTGTCAAAGTAGAGGTAAAACTTTTATGTCTGTGTCTGTTCTTTTTTCTGTAATTGAATCCGTAATAATTAGTTGGTATAATTAAATTTAACCCAACCCATTAACTTAAGTTCTTGAGTTTGGATTGATCGTTGATTTAACATAATTTAATATGGTATCAGAATAGAACAGTTTGAACCCATGTGAAATGTTAACTCCCAATTCATACTAACTTCCACATGTTGGGTGTTTCTCAAAATTTCAAGTCCATAAGTGAAGGGGAGTGTTAATTGATATAATTAAATTCACCCAACCCAACCCAACCGGCCAGTTTAAGCTTAGTTTGCGACCTGTGGTTGGTGTGGGACAAACTGTTTTCCTCTGTAATTTAACATCATTGGAGTCTAAATTCTCTGGTCATAATTGTTCAGGATGGTTCAATCTGGTTGTTTACTGCTGAGAAATTTGAGGGCTCTCGTCCATTCACGGTTCAGGCGAGCTATGAAGGTTTCTCGGAAGGTGCATTTTGAATTCTTTTTTCTCCCTTTGTGGTCTGTACTATGATTAGAAGAAATCTTTGTATCCCACAAATTGCTTTTTATAAATTTTGACAGGCACACAGGTGGGCGATTCAATTGTAATTGATGGGGGAATGGCAACCTTTGAAGTTATTGAAAAGATTGGGAATGATTTAAGCTGCAAGTGCACAGACCCTGGTTTGTTTCTTCCTAGAGCAAAGTGCAGCTTTTGGCGAGATGGAAGGCTTGTTGTGAGAAATCACGAGTCTCCCACTCTGTCAAGTAAGGTATGGAATATCAATATTTGTTCTGAATAATTTATTTGCGATGAATATGTCTGGAAGTTCTCACTTGTTGTTCTAGATCTGAGTATAGTGTTGGAACTTTCAAATATGGTATATTCAGATCAATGAGCCTTAGATGTTGCATTGTTTCTCTTGTGCATTTGTTTGGGATGTGAGCATCTGGACCAACTTACGTGCACTCAACTATTTTCACGCGACATACTGCCTGAGACTAATCCTACAATATTTGGATCCGAAGGAAATCTATAGAAAATTAATTTCTAAGTGTCTATAACCCATCACTTTTTGGTTTTCAAAATCCATTTTGAATACTAGGCCACTCGATAATGGCTGGTTAGGGATGATGAGTTCTTAACCCTCCCCAATGTCTTGACTCTTGGACATAATTTAAGGTTCACTGTTTGGAATTTTTTCTTACTCTGGCTTGTCAGCATTATTTTGGATACAATATGGTGACAATAATGTAAGGCCCATATCTAAAAAGTTGTCTTCTTATCGTTGATTGTCAGCGTTACAAGTTCTTGTTCCCGAATGATATTGTGGACCAAATTTAAGGTTCTTTTTTTTTAATATTATATTTGTATCAGTTGTCAGCGTCACAAGTTTTTATCCCTGTACATATCTCTTTCCAGAAGTGAAGTTATCCAATTTTCTGCTACATAAATTTAATTTGATTCAAAATTGGTTTTCTCTCCACTGACAAGGTTGAATTTTTTCTAAATTATATTTCTCCTTAGATGTTGTTGTATTAGATGGTACATGCATCCATCTCAAAATTAATTGATAATGAGTGGAGGCTCATCTATCTTATAAATACATGAAAGTCTCCTCACCTTTTTAATGTGAGATCCTCACCATGCCGGTTCTCTGCTTTTAACCGTGTTTTTGGTTTACTGTTTTACAGGATTGGTCCGACATTGAATTTGGAATTTCGGAAGGCGTCGACTTCATTGCCCTTTCCTTTGTGAACAGTGCAGATCCTATCAAGCATCTAAAGAACTACCTATCAAAAAAATCAGCAAAGTAAGCATATTGCTCCAAGAAGTTTACTTTTTAGGATGAGTTGGTTCCCCACTAAGCATGATCGTAAATCCCCACATCCCCTTCAATTGGATTTTACTGGTTTTTTAGCTGCAATTCATGAAATTGGTGCTCATATGTTGACAATATAGAGGCATTCTTTTCCCCAAAACTTGTCTTGTAAATTTGTATCACTGAAACAGATATTGTATAATTTCAAAACTTAATTTAATAATGAAGGATAAAAAATCTTTTGATGCACAGATCTGTGAGAGTATTGGCTAAGATTGAAAGCTTGGAGTCCCTTCGAAATCTGGAAGAAATTATAGAGGCTTCTGATGGAATCATGGTGGCTCGAGGGGATCTTGGGGTTGAAATTCCACTCGAGCAGATACCAGCAGTCCAAGAGGAAATAACTAGCGTCTGTAGGGAGCTGAATAAACCAGTGATCATAGCTTCTCAACTTCTCGAGTCGATGGTCGAATATCCTACTCCGACACGTGCTGAGGTACGAGTTACTTGTACATAAACGTATTTTCGAGTTGAAAATTCTCATGCTAGAAAATTGTGTTGAAGGCGCTAATGTGAGCTATACTCTGTAGATAATGCATCTATTACCCTTCCAAAGGTCATTAGTGTTGAAATGACTTGAACTAAGTTGTGCTACGATTACTCAATCGGAATCTCGCTGCCTGCTCATGGATTTATACTTCCGTATTGAGTTTTATGTATTTTGGGTCATATGTTGTATTAGAGTTTTATAAAAGTGCACTTTATGAACTCTTTTAAAACTCTAGTTCTCTTAGCCAAATTTTTTATATTCTTATTTCTAAATAAAACGGTTTCTCCTCTCTATCTATGGACGTAGCTAACACAACGTTAGCAAACCATGTAAATTTGTGTGTTTTTTGTTTTCTCGCTCTTTGATTCTTGTTTGCAGATAGTTTAATCTCACACCTTGTAGTTGCCAAAGAAATTTGTGTTGAAGGCTATAATTATGATCTTCTTTGTGTTCAATAGGTTGCAGATGTTTCTGAGGCAGTCAGGCAATATGCTGATGCATTGATGCTTTGTGGGGAGTCAGCCATCGGATCATATGGACAGAAAGCGCTTTCGGTTCTGCAGATGGCGAGCAGCCGAATGGAGTTATGGAGCCGCGAAGAAAATAGGAAAAATTTTATCCCCCAGCATCAACTTGGAGTGTCATTGCACGATCGCATTGCCGAAGAAATTTGCAATTCTGCTGCTGAATTGGGTAACACTCAGTTTTCTCCATCCACTTTTTAACAATGGGGTCATGAAAATAATATTTTCCTTTTATATCTGTGAGTAACTTACCTGCACATCAACTATTTTCACCGGGCACTACCCTACAACATTCAAATGCGAAGAAAATCCATAGAAAATTAATTTCTAGGTGACCATCATGACTTGAATCCATCACCTCCTAGTTTCATAAGACTCTCCAATTCATGATGGTTCATGAAAATAATGACAGAATTCCCTTTTGGCTATTACACAATCATGCAGAAAGAATGGTAACAAGAAAAGGATTAAATTACCATTTTAGTTTCAATTTGAAAATCTTGTTAGCTTATCTGTGAACACAAGTTTGTTAGGAAATAACTTTTATTTAAAGGGAATATAACCATTCTAAGTTAGCCTCATAGCAACCACGTCATGTAAATAAATAATAAAGGACTTAGAGGGAATAGATTCCAAATACAACCATTTACCTAAGTATTGAGATTTAGTATTGTACAAATCATCTTGGGAGTTAAATGTAGTAGGACTAGGTTAGTGTTCTGCACAAATAGTCGTAAGTTAGCCTGTACACTAACGGATATAAAAAATCGAGAAGTTCATGAATGGGAGGAAAAAGTGTTGTAAACAAAAGTTATTGAGATTTGGTAAAAAAAGATGTTGTGGGATTGTCATGGTGGTATGGTAGAAGTATAACTGATTCTTGCATCTTTACCTTTGAAACAAAAACTTCTATTTGTTTGACTGCTGCTAATTGCATTTGCTCAAATTTCCACCAAAACAAACACCTCCATGTTCTTTATGGCAGCCAACAGACTATCAGTAGACGCCATATTCGTGGTCACAAAGCAAGGGCACATGGCGTCTCTTCTCTCCCGGAACCGCCCAAGCCCACCCATATTCGCATTAACCGATGACAACAGCACGAGAATGGCTTTGAATCTTCAATGGGGAGTGTTTCCTCTGCGCGTTGACCTCTCAGACGACATTGAAGCCAACATCTCGAGAGGAATCGAGCTCGTGAAGTTGAAAGGGTTGGTCAAACAGGGGGACTGCGTGTTGGTGGTCTCGGAGATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAGTCAATCCAGCTGAAAACCATTGTGTAGTAGGAGACAGAGATAACCAAGGCAGGTCAGGTTTTGTTATTGGAAAGAACTTTGGGATGCTAGTTTGAGTGGTTTGTAATTGCATGTACCATATGTGGTGGTTAGTTATTTATCTAATTTTGTTCCTTGTTGTTTGTGGCCATGTGTTTATAATGACTGTTGAAAGCTTGGATAGGCTGTTTGTTGTGGTACCTAACATTTGGTAATTTAGTAAGTCAGAC

mRNA sequence

AAAATTTCAAAGGAGAGAAAAGGGTTACGGCGTCAGACAACAAAGCAGCAGAAACCAGTGGCCGACACGTGACAGTTACAGGTTCAACCGAACCACGAGCCAGCGCCGGGAAAGTTAGTTGACCGGTAATTTCAAATTAAATATAAATTTAATTTCTTAATTCAAAACCTAACAGCGCAGAGCGACAAAAGCCTTTGCCATTCTCCACAGCTAGGGTTCTGCCCAATAATCTCCTCTTCGAATATTCTTCTTTTTCTTGTAAAATCCACCATTGTTCGTAGTTACTCTTTGTTCAAATCTCGGCCGACAACCCGATTCAAACCCGATCCAAGGCCGAGGTAGGCTGTTGTCGTTGTCGTTGTCGTTGCTGCTGTTGTTCGTCAATGGCGGAGGTGAATGCTATGATGATTCGTGCTCGTTGCAGTTTCGTTGCGAAAATCCCTGTACTAGATGTGAAGCGTTCGGGTTTCGGTAAGCCGGTTATTCCTCGAACGGTTCGGTGCAATCGGAGGACGAATGGGAGAGTTGGAGCTGGAGTGAAGGCAGCAATGCAGGTGGGTTTGGAGGATGAGGAGAGGTTGAAGAGATTGGAGGGTTTAGAGGCGACGTTAGGGCTCGATGTCGTGGTGGAGAGGGAGTTGAAGGAGAAAGGGTTTTTGGGGATGAGGAAGACGAAGCTTGTTTGTACGATCGGCCCTGCGTGTTGTGCGGTCGAGGATTTGGAGAAGTTGGCTTTGGAAGGGATGAACATTGCGAGGCTGAATATGTGTCATAATACGAAGGAGTGGCACTGTGATGTGATTAAGAAGATTAACAAGTTGAATGAGGAGAAAGGGTTTTGTCTTTCTGTGATGATTGACACTGAAGGAAGTCAGATTCATGTTGTTGATCATGGAGCTCCTTCCTCTGTCAAAGTAGAGGATGGTTCAATCTGGTTGTTTACTGCTGAGAAATTTGAGGGCTCTCGTCCATTCACGGTTCAGGCGAGCTATGAAGGTTTCTCGGAAGGCACACAGGTGGGCGATTCAATTGTAATTGATGGGGGAATGGCAACCTTTGAAGTTATTGAAAAGATTGGGAATGATTTAAGCTGCAAGTGCACAGACCCTGGTTTGTTTCTTCCTAGAGCAAAGTGCAGCTTTTGGCGAGATGGAAGGCTTGTTGTGAGAAATCACGAGTCTCCCACTCTGTCAAGTAAGGATTGGTCCGACATTGAATTTGGAATTTCGGAAGGCGTCGACTTCATTGCCCTTTCCTTTGTGAACAGTGCAGATCCTATCAAGCATCTAAAGAACTACCTATCAAAAAAATCAGCAAAATCTGTGAGAGTATTGGCTAAGATTGAAAGCTTGGAGTCCCTTCGAAATCTGGAAGAAATTATAGAGGCTTCTGATGGAATCATGGTGGCTCGAGGGGATCTTGGGGTTGAAATTCCACTCGAGCAGATACCAGCAGTCCAAGAGGAAATAACTAGCGTCTGTAGGGAGCTGAATAAACCAGTGATCATAGCTTCTCAACTTCTCGAGTCGATGGTCGAATATCCTACTCCGACACGTGCTGAGGTTGCAGATGTTTCTGAGGCAGTCAGGCAATATGCTGATGCATTGATGCTTTGTGGGGAGTCAGCCATCGGATCATATGGACAGAAAGCGCTTTCGGTTCTGCAGATGGCGAGCAGCCGAATGGAGTTATGGAGCCGCGAAGAAAATAGGAAAAATTTTATCCCCCAGCATCAACTTGGAGTGTCATTGCACGATCGCATTGCCGAAGAAATTTGCAATTCTGCTGCTGAATTGGCCAACAGACTATCAGTAGACGCCATATTCGTGGTCACAAAGCAAGGGCACATGGCGTCTCTTCTCTCCCGGAACCGCCCAAGCCCACCCATATTCGCATTAACCGATGACAACAGCACGAGAATGGCTTTGAATCTTCAATGGGGAGTGTTTCCTCTGCGCGTTGACCTCTCAGACGACATTGAAGCCAACATCTCGAGAGGAATCGAGCTCGTGAAGTTGAAAGGGTTGGTCAAACAGGGGGACTGCGTGTTGGTGGTCTCGGAGATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAGTCAATCCAGCTGAAAACCATTGTGTAGTAGGAGACAGAGATAACCAAGGCAGGTCAGGTTTTGTTATTGGAAAGAACTTTGGGATGCTAGTTTGAGTGGTTTGTAATTGCATGTACCATATGTGGTGGTTAGTTATTTATCTAATTTTGTTCCTTGTTGTTTGTGGCCATGTGTTTATAATGACTGTTGAAAGCTTGGATAGGCTGTTTGTTGTGGTACCTAACATTTGGTAATTTAGTAAGTCAGAC

Coding sequence (CDS)

ATGGCGGAGGTGAATGCTATGATGATTCGTGCTCGTTGCAGTTTCGTTGCGAAAATCCCTGTACTAGATGTGAAGCGTTCGGGTTTCGGTAAGCCGGTTATTCCTCGAACGGTTCGGTGCAATCGGAGGACGAATGGGAGAGTTGGAGCTGGAGTGAAGGCAGCAATGCAGGTGGGTTTGGAGGATGAGGAGAGGTTGAAGAGATTGGAGGGTTTAGAGGCGACGTTAGGGCTCGATGTCGTGGTGGAGAGGGAGTTGAAGGAGAAAGGGTTTTTGGGGATGAGGAAGACGAAGCTTGTTTGTACGATCGGCCCTGCGTGTTGTGCGGTCGAGGATTTGGAGAAGTTGGCTTTGGAAGGGATGAACATTGCGAGGCTGAATATGTGTCATAATACGAAGGAGTGGCACTGTGATGTGATTAAGAAGATTAACAAGTTGAATGAGGAGAAAGGGTTTTGTCTTTCTGTGATGATTGACACTGAAGGAAGTCAGATTCATGTTGTTGATCATGGAGCTCCTTCCTCTGTCAAAGTAGAGGATGGTTCAATCTGGTTGTTTACTGCTGAGAAATTTGAGGGCTCTCGTCCATTCACGGTTCAGGCGAGCTATGAAGGTTTCTCGGAAGGCACACAGGTGGGCGATTCAATTGTAATTGATGGGGGAATGGCAACCTTTGAAGTTATTGAAAAGATTGGGAATGATTTAAGCTGCAAGTGCACAGACCCTGGTTTGTTTCTTCCTAGAGCAAAGTGCAGCTTTTGGCGAGATGGAAGGCTTGTTGTGAGAAATCACGAGTCTCCCACTCTGTCAAGTAAGGATTGGTCCGACATTGAATTTGGAATTTCGGAAGGCGTCGACTTCATTGCCCTTTCCTTTGTGAACAGTGCAGATCCTATCAAGCATCTAAAGAACTACCTATCAAAAAAATCAGCAAAATCTGTGAGAGTATTGGCTAAGATTGAAAGCTTGGAGTCCCTTCGAAATCTGGAAGAAATTATAGAGGCTTCTGATGGAATCATGGTGGCTCGAGGGGATCTTGGGGTTGAAATTCCACTCGAGCAGATACCAGCAGTCCAAGAGGAAATAACTAGCGTCTGTAGGGAGCTGAATAAACCAGTGATCATAGCTTCTCAACTTCTCGAGTCGATGGTCGAATATCCTACTCCGACACGTGCTGAGGTTGCAGATGTTTCTGAGGCAGTCAGGCAATATGCTGATGCATTGATGCTTTGTGGGGAGTCAGCCATCGGATCATATGGACAGAAAGCGCTTTCGGTTCTGCAGATGGCGAGCAGCCGAATGGAGTTATGGAGCCGCGAAGAAAATAGGAAAAATTTTATCCCCCAGCATCAACTTGGAGTGTCATTGCACGATCGCATTGCCGAAGAAATTTGCAATTCTGCTGCTGAATTGGCCAACAGACTATCAGTAGACGCCATATTCGTGGTCACAAAGCAAGGGCACATGGCGTCTCTTCTCTCCCGGAACCGCCCAAGCCCACCCATATTCGCATTAACCGATGACAACAGCACGAGAATGGCTTTGAATCTTCAATGGGGAGTGTTTCCTCTGCGCGTTGACCTCTCAGACGACATTGAAGCCAACATCTCGAGAGGAATCGAGCTCGTGAAGTTGAAAGGGTTGGTCAAACAGGGGGACTGCGTGTTGGTGGTCTCGGAGATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAGTCAATCCAGCTGAAAACCATTGTGTAG

Protein sequence

MAEVNAMMIRARCSFVAKIPVLDVKRSGFGKPVIPRTVRCNRRTNGRVGAGVKAAMQVGLEDEERLKRLEGLEATLGLDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLALEGMNIARLNMCHNTKEWHCDVIKKINKLNEEKGFCLSVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPIKHLKNYLSKKSAKSVRVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYGQKALSVLQMASSRMELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVVTKQGHMASLLSRNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISRGIELVKLKGLVKQGDCVLVVSEISPARAASMASQSIQLKTIV
Homology
BLAST of Sed0007460 vs. NCBI nr
Match: KAG7014927.1 (Plastidial pyruvate kinase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1042.7 bits (2695), Expect = 1.2e-300
Identity = 532/580 (91.72%), Postives = 553/580 (95.34%), Query Frame = 0

Query: 1   MAEVNAMMIRARCSFVAKIPVLDVKRSGFGKPVIPRTVRCNRRTNGRVGAGVKAAMQVGL 60
           MAEVNAMMIRA C+FVAK PVLDVKRSGFG PV  RTV CNRRT GRVG GVKA MQV L
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRRTTGRVGNGVKAVMQVDL 60

Query: 61  EDEERLKRLEGLEATLGLDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLALEG 120
           ED ERL+RLEGLEATLGLDVV ERELKEKGFLGMRKTKLVCTIGPAC A+EDLEKLALEG
Sbjct: 61  EDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEG 120

Query: 121 MNIARLNMCHNTKEWHCDVIKKINKLNEEKGFCLSVMIDTEGSQIHVVDHGAPSSVKVED 180
           MNIARLNMCHN++EWH DVI+KI KLNE KGFC+SVMIDTEGSQ+HVVDHGAPSS+KVED
Sbjct: 121 MNIARLNMCHNSREWHRDVIRKIKKLNEGKGFCISVMIDTEGSQVHVVDHGAPSSIKVED 180

Query: 181 GSIWLFTAEKFEGSRPFTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCKCT 240
           GSIWLFTAEKFEGSRPFTVQASYEGFSEG QVGDSIVIDGGMATFEVIEKIGNDLSC+CT
Sbjct: 181 GSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCRCT 240

Query: 241 DPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPIK 300
           DPGLFLPRAKCSFWRDGR+VVRNHESPTLSSKDWSDIEFGI EGVDFIALSFVNSADP+K
Sbjct: 241 DPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIVEGVDFIALSFVNSADPVK 300

Query: 301 HLKNYLSKKSAKSVRVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQE 360
           HLKNYLS KSAKSVRVLAKIESLESL+NLEEIIEASDGIMVARGDLGVEIPLEQIPAVQE
Sbjct: 301 HLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQE 360

Query: 361 EITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYG 420
           EITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALML GESAIGSYG
Sbjct: 361 EITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYG 420

Query: 421 QKALSVLQMASSRMELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVDAI 480
           QKALSVLQMAS RMELWSREENR+NF+P +QLGVSLHDRIAEEICNSAAELANRLSVDAI
Sbjct: 421 QKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSAAELANRLSVDAI 480

Query: 481 FVVTKQGHMASLLSRNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISRGI 540
           FV+T QGHMASLLSRNRP PPIFALTDD+STRMALNLQWGVFPLR++LS+DIEANI RGI
Sbjct: 481 FVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGI 540

Query: 541 ELVKLKGLVKQGDCVLVVSEISPARAASMASQSIQLKTIV 581
           ELVK KGLVKQGD VLVVSEISPARAASMASQSIQLKTIV
Sbjct: 541 ELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 580

BLAST of Sed0007460 vs. NCBI nr
Match: XP_038905899.1 (plastidial pyruvate kinase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1042.0 bits (2693), Expect = 2.0e-300
Identity = 535/581 (92.08%), Postives = 555/581 (95.52%), Query Frame = 0

Query: 1   MAEVNAMMIRARCSFVAKIPVLDVKRSGFGKPVIPRTVRCN-RRTNGRVGAGVKAAMQVG 60
           MAEVNA+MIR+R  F+AK PVLDVKRSGFG PVI RTV CN RRT G VG GVKA MQV 
Sbjct: 1   MAEVNAIMIRSRFDFIAKSPVLDVKRSGFGMPVIGRTVWCNRRRTRGGVGNGVKATMQVD 60

Query: 61  LEDEERLKRLEGLEATLGLDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLALE 120
           LEDEERLKRLEGLEATLGLDVV ERELKEKGFLGMRKTKLVCTIGPAC A+EDLEKLALE
Sbjct: 61  LEDEERLKRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACNAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNTKEWHCDVIKKINKLNEEKGFCLSVMIDTEGSQIHVVDHGAPSSVKVE 180
           GMNIARLNMCHN++EWHCDVI+KI KLNE+KGFC+SVMIDTEGSQIHVVDHGAPSSVKVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEKKGFCISVMIDTEGSQIHVVDHGAPSSVKVE 180

Query: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCKC 240
           DGSIWLFTAEKFEGSRPFTVQASYEGFSEG QVGDSIVIDGGMATFEVIEKIGNDLSC+C
Sbjct: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCRC 240

Query: 241 TDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPI 300
           TDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDI+FGISEGVDFIALSFVNSADP+
Sbjct: 241 TDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIKFGISEGVDFIALSFVNSADPV 300

Query: 301 KHLKNYLSKKSAKSVRVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360
           +HLKNYL  KSAKSVRVLAKIESLESL+NLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ
Sbjct: 301 QHLKNYLLTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALML GESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASSRMELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVDA 480
           GQKALSVLQMASSRME WSREENRKNF+PQHQLGVSLHDRIAEEICNSAAELANRLSVDA
Sbjct: 421 GQKALSVLQMASSRMESWSREENRKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVDA 480

Query: 481 IFVVTKQGHMASLLSRNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISRG 540
           IFV+T QGHMASLLSRNRPSP IFALTDD+STRMALNLQWGV PLR+D S+DIEANISRG
Sbjct: 481 IFVLTNQGHMASLLSRNRPSPLIFALTDDDSTRMALNLQWGVVPLRIDFSEDIEANISRG 540

Query: 541 IELVKLKGLVKQGDCVLVVSEISPARAASMASQSIQLKTIV 581
           IELVK KGLVK+GD VLVVSEISPARAASMASQSIQLKTIV
Sbjct: 541 IELVKSKGLVKKGDSVLVVSEISPARAASMASQSIQLKTIV 581

BLAST of Sed0007460 vs. NCBI nr
Match: XP_022922736.1 (plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1040.4 bits (2689), Expect = 5.8e-300
Identity = 528/580 (91.03%), Postives = 553/580 (95.34%), Query Frame = 0

Query: 1   MAEVNAMMIRARCSFVAKIPVLDVKRSGFGKPVIPRTVRCNRRTNGRVGAGVKAAMQVGL 60
           MAEVNAMMIRA C+FVAK PVLD+KRSGFG PV  RTV CNRRT GRVG GVKA MQV L
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDLKRSGFGMPVFGRTVWCNRRTTGRVGNGVKAVMQVDL 60

Query: 61  EDEERLKRLEGLEATLGLDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLALEG 120
           ED ERL+RLEGLEATLGLD+V ERELKEKGFLGMRKTKLVCTIGPAC A+EDLEKLALEG
Sbjct: 61  EDAERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEG 120

Query: 121 MNIARLNMCHNTKEWHCDVIKKINKLNEEKGFCLSVMIDTEGSQIHVVDHGAPSSVKVED 180
           MNIARLNMCHN++EWHCDVI+KI KLNE KGFC+SVMIDTEGSQIHVVDHGAPSS+KVED
Sbjct: 121 MNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVED 180

Query: 181 GSIWLFTAEKFEGSRPFTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCKCT 240
           GSIWLFTAEKFEGSRPFTVQASYEGFSEG QVGDSIVIDGGMATFEVIEKIGNDLSC+CT
Sbjct: 181 GSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCRCT 240

Query: 241 DPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPIK 300
           DPGLFLPRAKCSFWRDGR+VVRNHESPTLS KDWSDIEFGI+EGVDFIALSFVNSADP+K
Sbjct: 241 DPGLFLPRAKCSFWRDGRVVVRNHESPTLSRKDWSDIEFGIAEGVDFIALSFVNSADPVK 300

Query: 301 HLKNYLSKKSAKSVRVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQE 360
           HLKN+LS K AKSVRVLAKIESLESL+NLEEIIEAS+GIMVARGDLGVEIPLEQIPAVQE
Sbjct: 301 HLKNFLSTKPAKSVRVLAKIESLESLQNLEEIIEASEGIMVARGDLGVEIPLEQIPAVQE 360

Query: 361 EITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYG 420
           EITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALML GESAIGSYG
Sbjct: 361 EITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYG 420

Query: 421 QKALSVLQMASSRMELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVDAI 480
           QKALSVLQMAS RMELWSREENR+NF+P +QLGVSLHDRIAEEICNSAAELANRLSVDAI
Sbjct: 421 QKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSAAELANRLSVDAI 480

Query: 481 FVVTKQGHMASLLSRNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISRGI 540
           FV+T QGHMASLLSRNRP PPIFALTDD+STRMALNLQWGVFPLR++LS+DIEANI RGI
Sbjct: 481 FVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGI 540

Query: 541 ELVKLKGLVKQGDCVLVVSEISPARAASMASQSIQLKTIV 581
           ELVK KGLVKQGD VLVVSEISPARAASMASQSIQLKTIV
Sbjct: 541 ELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 580

BLAST of Sed0007460 vs. NCBI nr
Match: XP_023553283.1 (plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1037.7 bits (2682), Expect = 3.7e-299
Identity = 530/580 (91.38%), Postives = 553/580 (95.34%), Query Frame = 0

Query: 1   MAEVNAMMIRARCSFVAKIPVLDVKRSGFGKPVIPRTVRCN-RRTNGRVGAGVKAAMQVG 60
           MAEVNAMMIRA C+FVAK PVLDVKRSGFG PV  RTV CN RRT GRVG GVKA MQV 
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRRRTTGRVGNGVKAVMQVD 60

Query: 61  LEDEERLKRLEGLEATLGLDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLALE 120
           LED ERL+RLEGLEATLGLDVV ERELKEKGFLGMRKTKLVCTIGPAC A+EDLEKLALE
Sbjct: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNTKEWHCDVIKKINKLNEEKGFCLSVMIDTEGSQIHVVDHGAPSSVKVE 180
           GMNIARLNMCHN++EWHCDVI+KI KLNE KGFC+SVMIDTEGSQIHVVDHGAPSS+KVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180

Query: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCKC 240
           DGSIWLFTAEKFEGSRPFTVQASYEGFSEG QVGDS+VIDGGMATFEVIEKIGNDLSC+C
Sbjct: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRC 240

Query: 241 TDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPI 300
           TDPGLFLPRAKCSFWRDGR+VVRNHESPTLSSKDWSDIEFGI+EGVDFIALSFVNSADP+
Sbjct: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPV 300

Query: 301 KHLKNYLSKKSAKSVRVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360
           KHLKNYLS KSA+SVRVLAKIESLESL+NLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ
Sbjct: 301 KHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALML GESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASSRMELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVDA 480
           GQKALSVLQMAS RMELWSREENR+NF+P +QLGVSL DRIAEEICNSAAELANRLSVDA
Sbjct: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDA 480

Query: 481 IFVVTKQGHMASLLSRNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISRG 540
           IFV+T QGHMASLLSRNRP PPIFALTDD+STRMALNLQWGVFPLR++LS+DIEANI RG
Sbjct: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540

Query: 541 IELVKLKGLVKQGDCVLVVSEISPARAASMASQSIQLKTI 580
           IELVK KGLVKQGD VLVVSEISPARAASMASQSIQLKTI
Sbjct: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 580

BLAST of Sed0007460 vs. NCBI nr
Match: KAA0044313.1 (plastidial pyruvate kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1031.2 bits (2665), Expect = 3.5e-297
Identity = 526/582 (90.38%), Postives = 552/582 (94.85%), Query Frame = 0

Query: 1   MAEVNAMMIRARCSFVAKIPVLDVKRSGFGKPVI-PRTVRCN-RRTNGRVGAGVKAAMQV 60
           MAEVNAMMIRARC+F+ + PVLDVKR GF  PV+  RTV CN RR  GRVG GVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  GLEDEERLKRLEGLEATLGLDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLAL 120
            L+DEERL+RLEGLEATLGLD+V ERELKEKGFLGMRKTKLVCTIGPAC  +EDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNTKEWHCDVIKKINKLNEEKGFCLSVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHN++EWHCD+IKKI KLNEEKGFC+SVMID EGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCK 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEG +VGDSIVIDGGMATFEVIEKIGNDLSC+
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 IKHLKNYLSKKSAKSVRVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           ++HLKNYL  KSAKS+RVLAKIESLE+L+NLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALML GESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN KNF+PQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVVTKQGHMASLLSRNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISR 540
           AIFV+T QGHMASLLSRNRP PPIFALTDD+STRMALNLQWGVFPLR+DLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKLKGLVKQGDCVLVVSEISPARAASMASQSIQLKTIV 581
           GIE+VK KGLVKQGD VLVVSEISPARA SMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Sed0007460 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 6.2e-172
Identity = 295/491 (60.08%), Postives = 377/491 (76.78%), Query Frame = 0

Query: 74  ATLGLDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLALEGMNIARLNMCHNTK 133
           +++ +D V E ELKE GF   R+TKLVCTIGPA C  E+LE LA+ GMN+AR+NMCH T+
Sbjct: 83  SSIEVDAVTETELKENGFRSTRRTKLVCTIGPATCGFEELEALAVGGMNVARINMCHGTR 142

Query: 134 EWHCDVIKKINKLNEEKGFCLSVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEG 193
           EWH  VI+++ +LNEEKGF +++M+DTEGS+IH+ D G  SS K EDG IW F+   ++ 
Sbjct: 143 EWHKSVIERVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDS 202

Query: 194 SRP-FTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKCS 253
            RP  T+  +Y+GF+E  +VGD +++DGGM  FEVIEKIG D+ C+CTDPGL LPRA  +
Sbjct: 203 PRPERTINVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLT 262

Query: 254 FWRDGRLV-VRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPIKHLKNYLSKKSA 313
           FWRDG LV  RN   PT+SSKDW DI+FGI+EGVDFIA+SFV SA+ I HLK+Y++ +S 
Sbjct: 263 FWRDGSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSR 322

Query: 314 KS-VRVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELN 373
            S + V+AKIES++SL+NLEEII ASDG MVARGDLG +IPLEQ+P+ Q+ I  VCR+LN
Sbjct: 323 DSDIAVIAKIESIDSLKNLEEIIRASDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLN 382

Query: 374 KPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYGQKALSVLQMA 433
           KPVI+ASQLLESM+EYPTPTRAEVADVSEAVRQ ADALML GESA+G Y +KAL+VL+  
Sbjct: 383 KPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRADALMLSGESAMGQYPEKALAVLRSV 442

Query: 434 SSRMELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVVTKQGHMA 493
           S R+E W REE     +    +G +  D I+EEICNSAA++AN L VDA+FV TK GHMA
Sbjct: 443 SVRIEKWWREEKHHEAMELPAIGSTYSDSISEEICNSAAKMANNLGVDALFVYTKDGHMA 502

Query: 494 SLLSRNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISRGIELVKLKGLVK 553
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  +DD+E+N+++   L+K +G++K
Sbjct: 503 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIK 562

Query: 554 QGDCVLVVSEI 562
            GD V+ VS++
Sbjct: 563 SGDLVIAVSDM 573

BLAST of Sed0007460 vs. ExPASy Swiss-Prot
Match: Q9LIK0 (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 1.1e-171
Identity = 297/487 (60.99%), Postives = 370/487 (75.98%), Query Frame = 0

Query: 78  LDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLALEGMNIARLNMCHNTKEWHC 137
           +D V E ELKE GF   R+TKL+CTIGPA C  E LE LA+ GMN+ARLNMCH T++WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 138 DVIKKINKLNEEKGFCLSVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRP- 197
            VI+ + +LNEEKGF +++M+DTEGS+IH+ D G  +S K EDG +W FT   F+ SRP 
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 198 FTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKCSFWRD 257
            T+  SY+GF+E  +VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  +FWRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 258 GRLV-VRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPIKHLKNYLSKKS-AKSV 317
           G LV  RN   PT+SSKDW DI+FGI+EGVDFIA+SFV SA+ I HLK+YL+ +S    +
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 318 RVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVI 377
            V+AKIES++SL NLEEII ASDG MVARGDLG +IPLEQ+PA Q+ I  VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 378 IASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYGQKALSVLQMASSRM 437
           +ASQLLESM+EYPTPTRAEVADVSEAVRQ +DALML GESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 438 ELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVVTKQGHMASLLS 497
           E W REE R   +P   +G S  D+I+EEICNSAA++AN L VDA+FV T  GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 498 RNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISRGIELVKLKGLVKQGDC 557
           R RP  PIFA T   S R  LNLQWG+ P R+  SDD+E+N+++   L+K +G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 558 VLVVSEI 562
           V+ VS++
Sbjct: 580 VIAVSDM 586

BLAST of Sed0007460 vs. ExPASy Swiss-Prot
Match: Q40545 (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 5.4e-168
Identity = 291/491 (59.27%), Postives = 374/491 (76.17%), Query Frame = 0

Query: 74  ATLGLDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLALEGMNIARLNMCHNTK 133
           +++ +D V E ELKE GF   R+TKL+CTIGPA C  E LE+LA  GMN+AR+NMCH T+
Sbjct: 93  SSIEVDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLERLAEGGMNVARINMCHGTR 152

Query: 134 EWHCDVIKKINKLNEEKGFCLSVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEG 193
           EWH  VI+++ +LNEEKGF +++M+DTEGS+IH+ D G  SS K EDG IW FT   F+ 
Sbjct: 153 EWHRMVIERLRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWNFTVRSFDP 212

Query: 194 SRP-FTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKCS 253
             P  TV  +Y+GF+E  +VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  +
Sbjct: 213 PLPERTVTVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLT 272

Query: 254 FWRDGRLV-VRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPIKHLKNYLSKKSA 313
           FWRDG+LV  RN   PT+SSKDW DI+FGI+EGVDFIA+SFV SA+ IKHLK+Y+  ++ 
Sbjct: 273 FWRDGKLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVIKHLKSYIQARAR 332

Query: 314 KS-VRVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELN 373
            S + V+AKIES++SL+NLEEII+ASDG MVARGDLG +IPLEQ+P+ Q++I  +CR+LN
Sbjct: 333 DSDISVIAKIESIDSLKNLEEIIQASDGAMVARGDLGAQIPLEQVPSEQQKIVQICRQLN 392

Query: 374 KPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYGQKALSVLQMA 433
           +PVI+ASQLLESM+EYP PTRAEVADVSEAVRQ  DALML GESA+G + +KAL+VL+  
Sbjct: 393 RPVIVASQLLESMIEYPIPTRAEVADVSEAVRQRGDALMLSGESAMGQFPEKALTVLRSV 452

Query: 434 SSRMELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVVTKQGHMA 493
           S R+E   RE+ R   I    +  S  D I+EEICNSAA++AN L VDA+FV TK GHMA
Sbjct: 453 SLRIERMWREQKRHEVIELPSIASSFSDSISEEICNSAAKMANNLEVDALFVYTKNGHMA 512

Query: 494 SLLSRNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISRGIELVKLKGLVK 553
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  SDD+E+N+++   L+K +G++K
Sbjct: 513 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLMPFRLSFSDDMESNLNKTFSLLKARGMIK 572

Query: 554 QGDCVLVVSEI 562
            GD ++ VS++
Sbjct: 573 SGDLIIAVSDM 583

BLAST of Sed0007460 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 2.2e-84
Identity = 185/469 (39.45%), Postives = 275/469 (58.64%), Query Frame = 0

Query: 95  RKTKLVCTIGPACCAVEDLEKLALEGMNIARLNMCHNTKEWH---CDVIKKINKLNEEKG 154
           RKTK+VCTIGP+  + E + KLA  GMN+ARLNM H     H    D++K+ N   E+K 
Sbjct: 91  RKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFEDK- 150

Query: 155 FCLSVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGTQ 214
             +++M+DT+G +  V+    P  + +++G  + F+ ++   +   TV  +Y+ F    +
Sbjct: 151 -VIAIMLDTKGPE--VISGDVPKPILLKEGQEFNFSIKRGVSTED-TVSVNYDDFINDVE 210

Query: 215 VGDSIVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVVRNHES--PTL 274
            GD +++DGGM +  V  K  + + C+  D G    R          L VR   +  P++
Sbjct: 211 AGDILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSRR--------HLNVRGKSATLPSI 270

Query: 275 SSKDWSDIEFGISEGVDFIALSFVNSADPIKHLKNYLSKKSAKSVRVLAKIESLESLRNL 334
           + KDW DI+FG++  VDF A+SFV  A  +  LK+YL   +A  + V+ KIES +S+ NL
Sbjct: 271 TEKDWDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYLKSCNA-DIHVIVKIESADSIPNL 330

Query: 335 EEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIIASQLLESMVEYPTP 394
             II ASDG MVARGDLG E+P+E++P +QE+I   C+ + KPVI+A+ +LESM+++PTP
Sbjct: 331 HSIISASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPTP 390

Query: 395 TRAEVADVSEAVRQYADALMLCGESAIGSYGQKALSVLQMASSRMELWSREENRKNFIPQ 454
           TRAEV+D+S AVR+ ADA+ML GE+A G Y  KA+ V+ + + R E       +    P 
Sbjct: 391 TRAEVSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALRTE---SSLQKSTSSPS 450

Query: 455 HQLGVSLHDRIAEEICNSAAELANRLSVDAIFVVTKQGHMASLLSRNRPSPPIFALTDDN 514
                  H  + E     ++ +AN LS   I V T+ G MA +LS NRPS  +FA T++ 
Sbjct: 451 QSAAYKSH--MGEMFAFHSSSMANTLST-PIIVFTRTGSMAIILSHNRPSSTVFAFTNNE 510

Query: 515 STRMALNLQWGVFPLRVDLSDDIEANISRGIELVKLKGLVKQGDCVLVV 559
             +  L L  GV P+ ++ S D E   SR I+L+  K LVK G  V +V
Sbjct: 511 RVKQRLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSLVKDGQYVTLV 539

BLAST of Sed0007460 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 4.1e-83
Identity = 208/577 (36.05%), Postives = 316/577 (54.77%), Query Frame = 0

Query: 14  SFVAKIPVLDVKRSG----FGKPVIPRTVRCNRRTNGRVGAGVKAAMQVGLED----EER 73
           SF  K+   + KRSG      + V+  TVR     + RV   V   + V  ED    EE+
Sbjct: 34  SFAVKVLGNEAKRSGRVSVRSRRVVDTTVR-----SARVETEV---IPVSPEDVPNREEQ 93

Query: 74  LKRLEGLEATLGLDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLALEGMNIAR 133
           L+RL  LE     D  V    K       RKTK+VCT+GP+    E + KLA  GMN+AR
Sbjct: 94  LERL--LEMQQFGDTSVGMWSKP---TVRRKTKIVCTVGPSTNTREMIWKLAEAGMNVAR 153

Query: 134 LNMCHNTKEWHCDVIKKINKLN-EEKGFCLSVMIDTEGSQIHVVDHGAPSSVKVEDGSIW 193
           +NM H     H  VI  + + N + K   +++M+DT+G ++   D   P  + ++ G  +
Sbjct: 154 MNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPEVRSGD--LPQPIMLDPGQEF 213

Query: 194 LFTAEKFEGSRPFTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCKCTDPGL 253
            FT E+   S P  V  +Y+ F    + GD +++DGGM +F V  K  + + C+  D G 
Sbjct: 214 TFTIER-GVSTPSCVSVNYDDFVNDVEAGDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGE 273

Query: 254 FLPRAKCSFWRDGRLVVRNHES--PTLSSKDWSDIEFGISEGVDFIALSFVNSADPIKHL 313
              R          L VR   +  P+++ KDW DI+FG+   VDF A+SFV  A  +  L
Sbjct: 274 LKSRR--------HLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAVSFVKDAQVVHEL 333

Query: 314 KNYLSKKSAKSVRVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEI 373
           K YL + S   + V+ KIES +S+ NL  II ASDG MVARGDLG E+P+E++P +QEEI
Sbjct: 334 KKYL-QNSGADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPILQEEI 393

Query: 374 TSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYGQK 433
            ++CR + K VI+A+ +LESM+ +PTPTRAEV+D++ AVR+ ADA+ML GE+A G +  K
Sbjct: 394 INLCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLK 453

Query: 434 ALSVLQMASSRMELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFV 493
           A  V+   + R E           +P + LG +  + ++E     A  ++N L    + V
Sbjct: 454 AAGVMHTVALRTEATITSGE----MPPN-LGQAFKNHMSEMFAYHATMMSNTLGTSTV-V 513

Query: 494 VTKQGHMASLLSRNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISRGIEL 553
            T+ G MA LLS  RPS  I+A T++   +  L L  GV P+ ++ +DD E   +  +  
Sbjct: 514 FTRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANALAT 573

Query: 554 VKLKGLVKQGDCVLVVSEISPARAASMASQSIQLKTI 580
           +  +G+VK+G+ + +V   +     S ++ +IQ++ +
Sbjct: 574 LLKQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

BLAST of Sed0007460 vs. ExPASy TrEMBL
Match: A0A6J1E4Y3 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430639 PE=3 SV=1)

HSP 1 Score: 1040.4 bits (2689), Expect = 2.8e-300
Identity = 528/580 (91.03%), Postives = 553/580 (95.34%), Query Frame = 0

Query: 1   MAEVNAMMIRARCSFVAKIPVLDVKRSGFGKPVIPRTVRCNRRTNGRVGAGVKAAMQVGL 60
           MAEVNAMMIRA C+FVAK PVLD+KRSGFG PV  RTV CNRRT GRVG GVKA MQV L
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDLKRSGFGMPVFGRTVWCNRRTTGRVGNGVKAVMQVDL 60

Query: 61  EDEERLKRLEGLEATLGLDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLALEG 120
           ED ERL+RLEGLEATLGLD+V ERELKEKGFLGMRKTKLVCTIGPAC A+EDLEKLALEG
Sbjct: 61  EDAERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEG 120

Query: 121 MNIARLNMCHNTKEWHCDVIKKINKLNEEKGFCLSVMIDTEGSQIHVVDHGAPSSVKVED 180
           MNIARLNMCHN++EWHCDVI+KI KLNE KGFC+SVMIDTEGSQIHVVDHGAPSS+KVED
Sbjct: 121 MNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVED 180

Query: 181 GSIWLFTAEKFEGSRPFTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCKCT 240
           GSIWLFTAEKFEGSRPFTVQASYEGFSEG QVGDSIVIDGGMATFEVIEKIGNDLSC+CT
Sbjct: 181 GSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCRCT 240

Query: 241 DPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPIK 300
           DPGLFLPRAKCSFWRDGR+VVRNHESPTLS KDWSDIEFGI+EGVDFIALSFVNSADP+K
Sbjct: 241 DPGLFLPRAKCSFWRDGRVVVRNHESPTLSRKDWSDIEFGIAEGVDFIALSFVNSADPVK 300

Query: 301 HLKNYLSKKSAKSVRVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQE 360
           HLKN+LS K AKSVRVLAKIESLESL+NLEEIIEAS+GIMVARGDLGVEIPLEQIPAVQE
Sbjct: 301 HLKNFLSTKPAKSVRVLAKIESLESLQNLEEIIEASEGIMVARGDLGVEIPLEQIPAVQE 360

Query: 361 EITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYG 420
           EITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALML GESAIGSYG
Sbjct: 361 EITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYG 420

Query: 421 QKALSVLQMASSRMELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVDAI 480
           QKALSVLQMAS RMELWSREENR+NF+P +QLGVSLHDRIAEEICNSAAELANRLSVDAI
Sbjct: 421 QKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSAAELANRLSVDAI 480

Query: 481 FVVTKQGHMASLLSRNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISRGI 540
           FV+T QGHMASLLSRNRP PPIFALTDD+STRMALNLQWGVFPLR++LS+DIEANI RGI
Sbjct: 481 FVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGI 540

Query: 541 ELVKLKGLVKQGDCVLVVSEISPARAASMASQSIQLKTIV 581
           ELVK KGLVKQGD VLVVSEISPARAASMASQSIQLKTIV
Sbjct: 541 ELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 580

BLAST of Sed0007460 vs. ExPASy TrEMBL
Match: A0A5A7TLC2 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00350 PE=3 SV=1)

HSP 1 Score: 1031.2 bits (2665), Expect = 1.7e-297
Identity = 526/582 (90.38%), Postives = 552/582 (94.85%), Query Frame = 0

Query: 1   MAEVNAMMIRARCSFVAKIPVLDVKRSGFGKPVI-PRTVRCN-RRTNGRVGAGVKAAMQV 60
           MAEVNAMMIRARC+F+ + PVLDVKR GF  PV+  RTV CN RR  GRVG GVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  GLEDEERLKRLEGLEATLGLDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLAL 120
            L+DEERL+RLEGLEATLGLD+V ERELKEKGFLGMRKTKLVCTIGPAC  +EDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNTKEWHCDVIKKINKLNEEKGFCLSVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHN++EWHCD+IKKI KLNEEKGFC+SVMID EGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCK 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEG +VGDSIVIDGGMATFEVIEKIGNDLSC+
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 IKHLKNYLSKKSAKSVRVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           ++HLKNYL  KSAKS+RVLAKIESLE+L+NLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALML GESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN KNF+PQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVVTKQGHMASLLSRNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISR 540
           AIFV+T QGHMASLLSRNRP PPIFALTDD+STRMALNLQWGVFPLR+DLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKLKGLVKQGDCVLVVSEISPARAASMASQSIQLKTIV 581
           GIE+VK KGLVKQGD VLVVSEISPARA SMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Sed0007460 vs. ExPASy TrEMBL
Match: A0A5D3E0J9 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00350 PE=3 SV=1)

HSP 1 Score: 1029.2 bits (2660), Expect = 6.4e-297
Identity = 525/582 (90.21%), Postives = 551/582 (94.67%), Query Frame = 0

Query: 1   MAEVNAMMIRARCSFVAKIPVLDVKRSGFGKPVI-PRTVRCN-RRTNGRVGAGVKAAMQV 60
           MAEVNAMMIRARC+F+ + PVLDVKR GF  PV+  RTV CN RR  GRVG GVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  GLEDEERLKRLEGLEATLGLDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLAL 120
            L+DEERL+RLEGLEATLGLD+V ERELKEKGFLGMRKTKLVCTIGPAC  +EDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNTKEWHCDVIKKINKLNEEKGFCLSVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHN++EWHCD+IKKI KLNEEKGFC+SVMID EGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCK 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEG +VGDSIVIDGGMATFEV EKIGNDLSC+
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 IKHLKNYLSKKSAKSVRVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           ++HLKNYL  KSAKS+RVLAKIESLE+L+NLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALML GESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN KNF+PQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVVTKQGHMASLLSRNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISR 540
           AIFV+T QGHMASLLSRNRP PPIFALTDD+STRMALNLQWGVFPLR+DLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKLKGLVKQGDCVLVVSEISPARAASMASQSIQLKTIV 581
           GIE+VK KGLVKQGD VLVVSEISPARA SMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Sed0007460 vs. ExPASy TrEMBL
Match: E5GBH7 (Pyruvate kinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1029.2 bits (2660), Expect = 6.4e-297
Identity = 525/582 (90.21%), Postives = 551/582 (94.67%), Query Frame = 0

Query: 1   MAEVNAMMIRARCSFVAKIPVLDVKRSGFGKPVI-PRTVRCN-RRTNGRVGAGVKAAMQV 60
           MAEVNAMMIRARC+F+ + PVLDVKR GF  PV+  RTV CN RR  GRVG GVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  GLEDEERLKRLEGLEATLGLDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLAL 120
            L+DEERL+RLEGLEATLGLD+V ERELKEKGFLGMRKTKLVCTIGPAC  +EDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNTKEWHCDVIKKINKLNEEKGFCLSVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHN++EWHCD+IKKI KLNEEKGFC+SVMID EGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCK 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEG +VGDSIVIDGGMATFEV EKIGNDLSC+
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 IKHLKNYLSKKSAKSVRVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           ++HLKNYL  KSAKS+RVLAKIESLE+L+NLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALML GESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN KNF+PQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVVTKQGHMASLLSRNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISR 540
           AIFV+T QGHMASLLSRNRP PPIFALTDD+STRMALNLQWGVFPLR+DLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKLKGLVKQGDCVLVVSEISPARAASMASQSIQLKTIV 581
           GIE+VK KGLVKQGD VLVVSEISPARA SMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Sed0007460 vs. ExPASy TrEMBL
Match: A0A1S3BYK3 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103494833 PE=3 SV=1)

HSP 1 Score: 1029.2 bits (2660), Expect = 6.4e-297
Identity = 525/582 (90.21%), Postives = 551/582 (94.67%), Query Frame = 0

Query: 1   MAEVNAMMIRARCSFVAKIPVLDVKRSGFGKPVI-PRTVRCN-RRTNGRVGAGVKAAMQV 60
           MAEVNAMMIRARC+F+ + PVLDVKR GF  PV+  RTV CN RR  GRVG GVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  GLEDEERLKRLEGLEATLGLDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLAL 120
            L+DEERL+RLEGLEATLGLD+V ERELKEKGFLGMRKTKLVCTIGPAC  +EDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNTKEWHCDVIKKINKLNEEKGFCLSVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHN++EWHCD+IKKI KLNEEKGFC+SVMID EGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCK 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEG +VGDSIVIDGGMATFEV EKIGNDLSC+
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 IKHLKNYLSKKSAKSVRVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           ++HLKNYL  KSAKS+RVLAKIESLE+L+NLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALML GESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN KNF+PQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVVTKQGHMASLLSRNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISR 540
           AIFV+T QGHMASLLSRNRP PPIFALTDD+STRMALNLQWGVFPLR+DLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKLKGLVKQGDCVLVVSEISPARAASMASQSIQLKTIV 581
           GIE+VK KGLVKQGD VLVVSEISPARA SMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Sed0007460 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 604.7 bits (1558), Expect = 7.5e-173
Identity = 297/487 (60.99%), Postives = 370/487 (75.98%), Query Frame = 0

Query: 78  LDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLALEGMNIARLNMCHNTKEWHC 137
           +D V E ELKE GF   R+TKL+CTIGPA C  E LE LA+ GMN+ARLNMCH T++WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 138 DVIKKINKLNEEKGFCLSVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRP- 197
            VI+ + +LNEEKGF +++M+DTEGS+IH+ D G  +S K EDG +W FT   F+ SRP 
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 198 FTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKCSFWRD 257
            T+  SY+GF+E  +VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  +FWRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 258 GRLV-VRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPIKHLKNYLSKKS-AKSV 317
           G LV  RN   PT+SSKDW DI+FGI+EGVDFIA+SFV SA+ I HLK+YL+ +S    +
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 318 RVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVI 377
            V+AKIES++SL NLEEII ASDG MVARGDLG +IPLEQ+PA Q+ I  VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 378 IASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYGQKALSVLQMASSRM 437
           +ASQLLESM+EYPTPTRAEVADVSEAVRQ +DALML GESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 438 ELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVVTKQGHMASLLS 497
           E W REE R   +P   +G S  D+I+EEICNSAA++AN L VDA+FV T  GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 498 RNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISRGIELVKLKGLVKQGDC 557
           R RP  PIFA T   S R  LNLQWG+ P R+  SDD+E+N+++   L+K +G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 558 VLVVSEI 562
           V+ VS++
Sbjct: 580 VIAVSDM 586

BLAST of Sed0007460 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 310.5 bits (794), Expect = 2.9e-84
Identity = 208/577 (36.05%), Postives = 316/577 (54.77%), Query Frame = 0

Query: 14  SFVAKIPVLDVKRSG----FGKPVIPRTVRCNRRTNGRVGAGVKAAMQVGLED----EER 73
           SF  K+   + KRSG      + V+  TVR     + RV   V   + V  ED    EE+
Sbjct: 34  SFAVKVLGNEAKRSGRVSVRSRRVVDTTVR-----SARVETEV---IPVSPEDVPNREEQ 93

Query: 74  LKRLEGLEATLGLDVVVERELKEKGFLGMRKTKLVCTIGPACCAVEDLEKLALEGMNIAR 133
           L+RL  LE     D  V    K       RKTK+VCT+GP+    E + KLA  GMN+AR
Sbjct: 94  LERL--LEMQQFGDTSVGMWSKP---TVRRKTKIVCTVGPSTNTREMIWKLAEAGMNVAR 153

Query: 134 LNMCHNTKEWHCDVIKKINKLN-EEKGFCLSVMIDTEGSQIHVVDHGAPSSVKVEDGSIW 193
           +NM H     H  VI  + + N + K   +++M+DT+G ++   D   P  + ++ G  +
Sbjct: 154 MNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPEVRSGD--LPQPIMLDPGQEF 213

Query: 194 LFTAEKFEGSRPFTVQASYEGFSEGTQVGDSIVIDGGMATFEVIEKIGNDLSCKCTDPGL 253
            FT E+   S P  V  +Y+ F    + GD +++DGGM +F V  K  + + C+  D G 
Sbjct: 214 TFTIER-GVSTPSCVSVNYDDFVNDVEAGDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGE 273

Query: 254 FLPRAKCSFWRDGRLVVRNHES--PTLSSKDWSDIEFGISEGVDFIALSFVNSADPIKHL 313
              R          L VR   +  P+++ KDW DI+FG+   VDF A+SFV  A  +  L
Sbjct: 274 LKSRR--------HLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAVSFVKDAQVVHEL 333

Query: 314 KNYLSKKSAKSVRVLAKIESLESLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEI 373
           K YL + S   + V+ KIES +S+ NL  II ASDG MVARGDLG E+P+E++P +QEEI
Sbjct: 334 KKYL-QNSGADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPILQEEI 393

Query: 374 TSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYGQK 433
            ++CR + K VI+A+ +LESM+ +PTPTRAEV+D++ AVR+ ADA+ML GE+A G +  K
Sbjct: 394 INLCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLK 453

Query: 434 ALSVLQMASSRMELWSREENRKNFIPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFV 493
           A  V+   + R E           +P + LG +  + ++E     A  ++N L    + V
Sbjct: 454 AAGVMHTVALRTEATITSGE----MPPN-LGQAFKNHMSEMFAYHATMMSNTLGTSTV-V 513

Query: 494 VTKQGHMASLLSRNRPSPPIFALTDDNSTRMALNLQWGVFPLRVDLSDDIEANISRGIEL 553
            T+ G MA LLS  RPS  I+A T++   +  L L  GV P+ ++ +DD E   +  +  
Sbjct: 514 FTRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANALAT 573

Query: 554 VKLKGLVKQGDCVLVVSEISPARAASMASQSIQLKTI 580
           +  +G+VK+G+ + +V   +     S ++ +IQ++ +
Sbjct: 574 LLKQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

BLAST of Sed0007460 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 306.6 bits (784), Expect = 4.2e-83
Identity = 190/492 (38.62%), Postives = 284/492 (57.72%), Query Frame = 0

Query: 95  RKTKLVCTIGPACCAVEDLEKLALEGMNIARLNMCHNTKEWH---CDVIKKINKLNEEKG 154
           RKTK+VCTIGP+  + E + KLA  GMN+ARLNM H     H    D++K+ N L  +K 
Sbjct: 99  RKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDK- 158

Query: 155 FCLSVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGTQ 214
             +++M+DT+G ++   D   P  + +E+G  + FT ++    +  TV  +Y+ F    +
Sbjct: 159 -AIAIMLDTKGPEVRSGD--VPQPIFLEEGQEFNFTIKRGVSLKD-TVSVNYDDFVNDVE 218

Query: 215 VGDSIVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVVRNHES--PTL 274
           VGD +++DGGM +  V  K  + + C   D G    R          L VR   +  P++
Sbjct: 219 VGDILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRR--------HLNVRGKSATLPSI 278

Query: 275 SSKDWSDIEFGISEGVDFIALSFVNSADPIKHLKNYLSKKSAKSVRVLAKIESLESLRNL 334
           + KDW DI+FG+   VDF A+SFV  A  +  LKNYL   SA  + V+ KIES +S++NL
Sbjct: 279 TDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYLKTCSA-DISVIVKIESADSIKNL 338

Query: 335 EEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIIASQLLESMVEYPTP 394
             II A DG MVARGDLG E+P+E++P +QEEI   CR ++KPVI+A+ +LESM+ +PTP
Sbjct: 339 PSIISACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPTP 398

Query: 395 TRAEVADVSEAVRQYADALMLCGESAIGSYGQKALSVLQMASSRME--LWSREENRKNFI 454
           TRAEV+D++ AVR+ ADA+ML GE+A G +  KA++V+   + R E  L  R    +   
Sbjct: 399 TRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLPVRTSASRTTA 458

Query: 455 PQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVVTKQGHMASLLSRNRPSPPIFALTD 514
            +  +G        +     A+ +AN LS   + V T+ G MA LLS  RPS  IFA T+
Sbjct: 459 YKGHMG--------QMFAFHASIMANTLS-SPLIVFTRTGSMAVLLSHYRPSATIFAFTN 518

Query: 515 DNSTRMALNLQWGVFPLRVDLSDDIEANISRGIELVKLKGLVKQGDCVLVVSEISPARAA 574
                  L L  GV P+ ++ SDD E   +R ++L++ + ++K+G  V +V   S     
Sbjct: 519 QRRIMQRLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWR 567

Query: 575 SMASQSIQLKTI 580
             ++  IQ++ I
Sbjct: 579 EESTHLIQVRKI 567

BLAST of Sed0007460 vs. TAIR 10
Match: AT3G04050.1 (Pyruvate kinase family protein )

HSP 1 Score: 225.7 bits (574), Expect = 9.5e-59
Identity = 161/507 (31.76%), Postives = 250/507 (49.31%), Query Frame = 0

Query: 96  KTKLVCTIGPACCAVEDLEKLALEGMNIARLNMCHNTKEWHCDVIKKINKLNEEKGFCLS 155
           KTK+VCT+GPA  +VE +EKL   GMN+AR N  H +  +H + +  +    E      +
Sbjct: 17  KTKIVCTLGPASRSVEMVEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAMENTCIPCA 76

Query: 156 VMIDTEGSQIHV--VDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGTQVG 215
           VM+DT+G +I    +  G P  + ++   I + T    EG    T+  SY+  +E  + G
Sbjct: 77  VMLDTKGPEIRTGFLKEGKPVEL-IQGQEITISTDYTMEGDSN-TISMSYKKLAEDLKSG 136

Query: 216 DSIVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVVRNH--------E 275
           D I+   G  +  V       LSC   D  L L RA+C       L  R +        +
Sbjct: 137 DVILCSDGTISLTV-------LSC---DKNLGLVRARCE--NSAVLGERKNVNLPGIVVD 196

Query: 276 SPTLSSKDWSDI-EFGISEGVDFIALSFVNSADPIKHLKNYLSKKSAKSVRVLAKIESLE 335
            PTL+ KD  DI ++G+   +D IALSFV     +  ++  L  ++AKS+ +++K+E+ E
Sbjct: 197 LPTLTEKDQEDILQWGVPNKIDIIALSFVRKGSDLVEVRKLLG-ENAKSIMLMSKVENQE 256

Query: 336 SLRNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIIASQLLESMV 395
            + N ++I+E SD  MVARGDLG+EIP+E++   Q+ +      L KP++ A+Q+LESM 
Sbjct: 257 GVMNFDKILEYSDAFMVARGDLGMEIPIEKMFLAQKMMIQKANALGKPIVTATQMLESMT 316

Query: 396 EYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYGQKALSVLQMASSRMELWSREENRK 455
           + P PTRAE  DV+ AV    D +ML GE+A G++ + A+  +       E         
Sbjct: 317 KSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAHPETAVLTMSRICKEAE--------- 376

Query: 456 NFIPQHQLGVSLHDRIA------EEICNSAAELANRLSVDAIFVVTKQGHMASLLSRNRP 515
           +FI    +   + D ++      E +  SA   A  L   AI V+TK G+   L+++ RP
Sbjct: 377 DFIDYDTMHKKIQDIVSLPLSPIESLAASAVSTARSLCAAAIVVLTKGGYTVELVAKYRP 436

Query: 516 SPPIFALTDDNSTRMALNLQW-----------------GVFPL-------RVDLSDDIEA 562
           S PI ++     TR   + +W                 G+ P+       R    D  E 
Sbjct: 437 SVPILSVIVPEITRTD-DFEWSCSESAAHVARRGLIYRGIVPVMATGASARSSNKDSTEE 496

BLAST of Sed0007460 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 220.7 bits (561), Expect = 3.1e-57
Identity = 155/494 (31.38%), Postives = 252/494 (51.01%), Query Frame = 0

Query: 96  KTKLVCTIGPACCAVEDLEKLALEGMNIARLNMCHNTKEWHCDVIKKINKLNEEKGFCLS 155
           KTK+VCT+GPA  +V  +EKL   GMN+AR N  H + E+H + +  +    +  G   +
Sbjct: 21  KTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLRTAMQNTGILAA 80

Query: 156 VMIDTEGSQIHV--VDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGTQVG 215
           VM+DT+G +I    +  G P  +K E   I + T    +G    T+  SY+      + G
Sbjct: 81  VMLDTKGPEIRTGFLKDGNPIQLK-EGQEITITTDYDIKGDEK-TISMSYKKLPVDVKPG 140

Query: 216 DSIVIDGGMATFEVI--EKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSS 275
           ++I+   G  +  V+  +     + C+C +  +   R   +    G +V    + PTL+ 
Sbjct: 141 NTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKNVNL--PGVVV----DLPTLTD 200

Query: 276 KDWSDI-EFGISEGVDFIALSFVNSADPIKHLKNYLSKKSAKSVRVLAKIESLESLRNLE 335
           KD  DI ++G+   +D IALSFV     + +++  L   S KS+ +++K+E+ E + N +
Sbjct: 201 KDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGSHS-KSIMLMSKVENQEGVLNFD 260

Query: 336 EIIEASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIIASQLLESMVEYPTPT 395
           EI+  +D  MVARGDLG+EIP+E+I   Q+ +   C    KPV+ A+Q+LESM++ P PT
Sbjct: 261 EILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPT 320

Query: 396 RAEVADVSEAVRQYADALMLCGESAIGSYGQKALSVLQMASSRMELWSREENRKNFIPQH 455
           RAE  DV+ AV    D +ML GESA G+Y +  ++V  MA   +E  S  +    F  + 
Sbjct: 321 RAEATDVANAVLDGTDCVMLSGESAAGAYPE--IAVKTMAKICIEAESSLDYNTIF-KEM 380

Query: 456 QLGVSLHDRIAEEICNSAAELANRLSVDAIFVVTKQGHMASLLSRNRPSPPIFAL----- 515
                L     E + +SA   AN+     I V+T+ G  A L+++ RP+ PI ++     
Sbjct: 381 IRATPLPMSTLESLASSAVRTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPILSVVVPVF 440

Query: 516 ---------TDDNSTRMALNLQWGVFPL------RVDLSDDIEANISRGIELVKLKGLVK 565
                    +D++  R +L +  G+ P+      +   S+  E  I   ++    KGL  
Sbjct: 441 TSDTFNWSCSDESPARHSL-IYRGLIPVLGEGSAKATDSESTEEIIESALKSATEKGLCN 500

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7014927.11.2e-30091.72Plastidial pyruvate kinase 1, chloroplastic [Cucurbita argyrosperma subsp. argyr... [more]
XP_038905899.12.0e-30092.08plastidial pyruvate kinase 1, chloroplastic-like [Benincasa hispida][more]
XP_022922736.15.8e-30091.03plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita moschata][more]
XP_023553283.13.7e-29991.38plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAA0044313.13.5e-29790.38plastidial pyruvate kinase 1 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q431176.2e-17260.08Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1[more]
Q9LIK01.1e-17160.99Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q405455.4e-16859.27Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q405462.2e-8439.45Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9FLW94.1e-8336.05Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E4Y32.8e-30091.03Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430639 PE=3 SV=1[more]
A0A5A7TLC21.7e-29790.38Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00350... [more]
A0A5D3E0J96.4e-29790.21Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G0035... [more]
E5GBH76.4e-29790.21Pyruvate kinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S3BYK36.4e-29790.21Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103494833 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G22960.17.5e-17360.99Pyruvate kinase family protein [more]
AT5G52920.12.9e-8436.05plastidic pyruvate kinase beta subunit 1 [more]
AT1G32440.14.2e-8338.62plastidial pyruvate kinase 3 [more]
AT3G04050.19.5e-5931.76Pyruvate kinase family protein [more]
AT5G63680.13.1e-5731.38Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 343..367
score: 48.92
coord: 412..428
score: 35.11
coord: 152..168
score: 26.97
coord: 368..392
score: 57.66
coord: 285..299
score: 44.51
coord: 393..411
score: 48.99
coord: 316..342
score: 52.82
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 95..566
e-value: 2.9E-121
score: 403.2
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 38..579
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 435..561
e-value: 5.6E-139
score: 465.6
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 458..561
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 163..267
e-value: 5.6E-139
score: 465.6
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 95..434
e-value: 5.6E-139
score: 465.6
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 94..432
e-value: 3.4E-85
score: 286.0
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 462..562
e-value: 6.2E-20
score: 71.6
NoneNo IPR availablePANTHERPTHR11817:SF21PYRUVATE KINASEcoord: 38..579
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 314..326
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 165..252
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 89..435

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0007460.1Sed0007460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity