Sed0006658 (gene) Chayote v1

Overview


Name	Sed0006658
Type	gene
Organism	Sechium edule (Chayote v1)
Description	calcium-binding allergen Bet v 3-like
Location	LG02: 30792924 .. 30793639 (+)
RNA-Seq Expression	Sed0006658
Synteny	Sed0006658

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAGGGTCCCCGAGCTCGGCTGATGAAGAAACCGTCGTCCTTCCGGCTGCGCAGCCAGAGTCTAAATACCCTTCGCCTTCGTCGTATTTTTGACATGTTCGATAAGAACGGCGACGGCATGATCACCACAGAGGAACTAGGTCAAGCTCTGGGCCTTCTTGGGCTGGAAGCGGAGCAATCCGACTTGGAATCCACGGTCAGCTCCTTTCTCCAAACTGGAAACTCCGGCCTTCGATTCGAAGACTTTGCGGCCTTACACCAGTCTTTGGACGACACGTATTTCGGAGAGGAGGCCGAGGATGAAGAGAAGAAGAGTGAAGAAGAAGGAGGGTTAACGCCCATGTCGCAGGAGGAGTCTGATCTGTCAGAGGCATTCAGGGTGTTCGATGAGGATGGCGATGGCTACATCTCAGCTCTGGAGCTCCAAGTGGTTCTCAGAAAGCTCCGACTGGACGAGGGGAATGAGATCGACCGGGTTCAGAAGATGATTCTCTCGGTCGACCGCAATCACGACGGCCGCGTTGATTTCTTCGAGTTCAAGGACATGATGCGCAGTGTTATTGTCAAGAGTTTTTGATTCATTCCAATCCCCCATGCGCCTCCCCATCCATCCATCCATCTAACTCCCTTTTCAATATTTCTATTTCCTTTTCTTTTCTTTTCTAATATGTCTATCTCATTTTTCTCATCGCCAATTTTCCCCTTTACCCA

mRNA sequence

ATGGAAGAGGGTCCCCGAGCTCGGCTGATGAAGAAACCGTCGTCCTTCCGGCTGCGCAGCCAGAGTCTAAATACCCTTCGCCTTCGTCGTATTTTTGACATGTTCGATAAGAACGGCGACGGCATGATCACCACAGAGGAACTAGGTCAAGCTCTGGGCCTTCTTGGGCTGGAAGCGGAGCAATCCGACTTGGAATCCACGGTCAGCTCCTTTCTCCAAACTGGAAACTCCGGCCTTCGATTCGAAGACTTTGCGGCCTTACACCAGTCTTTGGACGACACGTATTTCGGAGAGGAGGCCGAGGATGAAGAGAAGAAGAGTGAAGAAGAAGGAGGGTTAACGCCCATGTCGCAGGAGGAGTCTGATCTGTCAGAGGCATTCAGGGTGTTCGATGAGGATGGCGATGGCTACATCTCAGCTCTGGAGCTCCAAGTGGTTCTCAGAAAGCTCCGACTGGACGAGGGGAATGAGATCGACCGGGTTCAGAAGATGATTCTCTCGGTCGACCGCAATCACGACGGCCGCGTTGATTTCTTCGAGTTCAAGGACATGATGCGCAGTGTTATTGTCAAGAGTTTTTGATTCATTCCAATCCCCCATGCGCCTCCCCATCCATCCATCCATCTAACTCCCTTTTCAATATTTCTATTTCCTTTTCTTTTCTTTTCTAATATGTCTATCTCATTTTTCTCATCGCCAATTTTCCCCTTTACCCA

Coding sequence (CDS)

ATGGAAGAGGGTCCCCGAGCTCGGCTGATGAAGAAACCGTCGTCCTTCCGGCTGCGCAGCCAGAGTCTAAATACCCTTCGCCTTCGTCGTATTTTTGACATGTTCGATAAGAACGGCGACGGCATGATCACCACAGAGGAACTAGGTCAAGCTCTGGGCCTTCTTGGGCTGGAAGCGGAGCAATCCGACTTGGAATCCACGGTCAGCTCCTTTCTCCAAACTGGAAACTCCGGCCTTCGATTCGAAGACTTTGCGGCCTTACACCAGTCTTTGGACGACACGTATTTCGGAGAGGAGGCCGAGGATGAAGAGAAGAAGAGTGAAGAAGAAGGAGGGTTAACGCCCATGTCGCAGGAGGAGTCTGATCTGTCAGAGGCATTCAGGGTGTTCGATGAGGATGGCGATGGCTACATCTCAGCTCTGGAGCTCCAAGTGGTTCTCAGAAAGCTCCGACTGGACGAGGGGAATGAGATCGACCGGGTTCAGAAGATGATTCTCTCGGTCGACCGCAATCACGACGGCCGCGTTGATTTCTTCGAGTTCAAGGACATGATGCGCAGTGTTATTGTCAAGAGTTTTTGA

Protein sequence

MEEGPRARLMKKPSSFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAEQSDLESTVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEEEGGLTPMSQEESDLSEAFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFFEFKDMMRSVIVKSF

Homology

[Close]

BLAST of Sed0006658 vs. NCBI nr
Match: XP_023527056.1 (calcium-binding allergen Bet v 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 290.4 bits (742), Expect = 1.1e-74
Identity = 156/193 (80.83%), Postives = 171/193 (88.60%), Query Frame = 0


Query: 5   PRARLMKKPS----SFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAE 64
           PR RL KKPS    SFRLR  SLN+LRLRRIFDMFDKNGDGMITTEELGQALGLLGL+A+
Sbjct: 78  PRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDAD 137

Query: 65  QSDLESTVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEE-EGGLTPMSQE 124
           +SDL+STV+SF+  GN GLRFEDFAALHQSL+DTYFGE  EDEE+K E+ E  ++P+SQE
Sbjct: 138 ESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPVEDEEEKEEKRETNMSPVSQE 197

Query: 125 ESDLSEAFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFF 184
           E DLSEAFRVFDEDGDGYISA ELQVVLRKLRL EGNE+DRVQKMILSVD N DGRVDFF
Sbjct: 198 ELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFF 257

Query: 185 EFKDMMRSVIVKS 193
           EFKDMMRSVIV+S
Sbjct: 258 EFKDMMRSVIVRS 270

[Close]

BLAST of Sed0006658 vs. NCBI nr
Match: XP_022982604.1 (calcium-binding allergen Bet v 3-like [Cucurbita maxima])

HSP 1 Score: 290.0 bits (741), Expect = 1.5e-74
Identity = 156/193 (80.83%), Postives = 171/193 (88.60%), Query Frame = 0


Query: 5   PRARLMKKPS----SFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAE 64
           PR RL KKPS    SFRLR  SLN+LRLRRIFDMFDKNGDGMITTEELGQALGLLGL+A+
Sbjct: 9   PRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDAD 68

Query: 65  QSDLESTVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEE-EGGLTPMSQE 124
           +SDL+STV+SF+  GN GLRFEDFAALHQSL+DTYFGE  EDEE+K E+ E  ++P+SQE
Sbjct: 69  ESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRETNMSPVSQE 128

Query: 125 ESDLSEAFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFF 184
           E DLSEAFRVFDEDGDGYISA ELQVVLRKLRL EGNE+DRVQKMILSVD N DGRVDFF
Sbjct: 129 ELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFF 188

Query: 185 EFKDMMRSVIVKS 193
           EFKDMMRSVIV+S
Sbjct: 189 EFKDMMRSVIVRS 201

[Close]

BLAST of Sed0006658 vs. NCBI nr
Match: XP_004137668.1 (calcium-binding protein CML42 [Cucumis sativus] >KGN58678.1 hypothetical protein Csa_002212 [Cucumis sativus])

HSP 1 Score: 288.9 bits (738), Expect = 3.3e-74
Identity = 153/192 (79.69%), Postives = 170/192 (88.54%), Query Frame = 0


Query: 5   PRARLMKKP----SSFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAE 64
           PR RL KKP    SSFRLR  SLN+LRLRRIFDMFDKNGDGMITT+ELGQALGLLGL+A+
Sbjct: 9   PRPRLTKKPSSLSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTDELGQALGLLGLDAD 68

Query: 65  QSDLESTVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEEEGGLTPMSQEE 124
           +S+L+STV+SF++ GN GLRFEDFAALHQSL+DTYFG   EDE++  + E  ++ MSQEE
Sbjct: 69  ESELDSTVNSFIEPGNEGLRFEDFAALHQSLNDTYFGGMVEDEDEDEKRETSMSSMSQEE 128

Query: 125 SDLSEAFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFFE 184
           SDLSEAFRVFDEDGDGYISA ELQVVLRKLRL EGNEIDRVQKMILSVDRN DGRVDFFE
Sbjct: 129 SDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFE 188

Query: 185 FKDMMRSVIVKS 193
           FKDMMRSVIV+S
Sbjct: 189 FKDMMRSVIVRS 200

[Close]

BLAST of Sed0006658 vs. NCBI nr
Match: KAG6580420.1 (Calcium-binding allergen Bet v 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 287.3 bits (734), Expect = 9.5e-74
Identity = 155/193 (80.31%), Postives = 170/193 (88.08%), Query Frame = 0


Query: 5   PRARLMKKPS----SFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAE 64
           PR RL KKPS    SFRLR  SLN+LRLRRIFDMFDKNGDGMITTEELGQALGLLGL+A+
Sbjct: 9   PRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDAD 68

Query: 65  QSDLESTVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEE-EGGLTPMSQE 124
           +SDL+STV+SF+  GN GLRFEDFAALHQSL+DTYF E  EDEE+K E+ E  ++P+SQE
Sbjct: 69  ESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFREPVEDEEEKEEKRETNMSPVSQE 128

Query: 125 ESDLSEAFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFF 184
           E DLSEAFRVFDEDGDGYISA ELQVVLRKLRL EGNE+DRVQKMILSVD N DGRVDFF
Sbjct: 129 ELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFF 188

Query: 185 EFKDMMRSVIVKS 193
           EFKDMMRSVIV+S
Sbjct: 189 EFKDMMRSVIVRS 201

[Close]

BLAST of Sed0006658 vs. NCBI nr
Match: XP_022935065.1 (calcium-binding allergen Bet v 3-like [Cucurbita moschata])

HSP 1 Score: 285.0 bits (728), Expect = 4.7e-73
Identity = 154/193 (79.79%), Postives = 169/193 (87.56%), Query Frame = 0


Query: 5   PRARLMKKPS----SFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAE 64
           PR RL KKPS    SFRLR  SLN+LRLRRIFDMFDKNGDGMITTEELGQALGLLGL+A+
Sbjct: 9   PRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDAD 68

Query: 65  QSDLESTVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEE-EGGLTPMSQE 124
           +SDL+STV+SF+  GN GLRFEDFAALHQ L+DTYF E  EDEE+K E+ E  ++P+SQE
Sbjct: 69  ESDLDSTVNSFIAPGNVGLRFEDFAALHQFLNDTYFREPVEDEEEKEEKRETNMSPVSQE 128

Query: 125 ESDLSEAFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFF 184
           E DLSEAFRVFDEDGDGYISA ELQVVLRKLRL EGNE+DRVQKMILSVD N DGRVDFF
Sbjct: 129 ELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFF 188

Query: 185 EFKDMMRSVIVKS 193
           EFKDMMRSVIV+S
Sbjct: 189 EFKDMMRSVIVRS 201

[Close]

BLAST of Sed0006658 vs. ExPASy Swiss-Prot
Match: P43187 (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 4.0e-59
Identity = 125/188 (66.49%), Postives = 151/188 (80.32%), Query Frame = 0


Query: 5   PRARLMKKPSSFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAEQSDL 64
           PR R +   SSFRLRS+SLNTLRLRRIFD+FDKN DG+IT +EL +AL LLGLE + S+L
Sbjct: 19  PRKRSLSN-SSFRLRSESLNTLRLRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSEL 78

Query: 65  ESTVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEEEGGLTPMSQEESDLS 124
           ESTV SF + GN GL+FEDF +LHQSL+D+YF    EDE+  +EE+   + +SQEE+D  
Sbjct: 79  ESTVKSFTREGNIGLQFEDFISLHQSLNDSYFAYGGEDED-DNEEDMRKSILSQEEADSF 138

Query: 125 EAFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFFEFKDM 184
             F+VFDEDGDGYISA ELQ+VL KL   EG+EIDRV+KMI+SVD N DGRVDFFEFKDM
Sbjct: 139 GGFKVFDEDGDGYISARELQMVLGKLGFSEGSEIDRVEKMIVSVDSNRDGRVDFFEFKDM 198

Query: 185 MRSVIVKS 193
           MRSV+V+S
Sbjct: 199 MRSVLVRS 204

[Close]

BLAST of Sed0006658 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 2.7e-55
Identity = 112/187 (59.89%), Postives = 148/187 (79.14%), Query Frame = 0


Query: 6   RARLMKKPSSFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAEQSDLE 65
           + ++ ++ SSFRLRS SLN LRL+RIFD+FDKNGDG IT EEL QAL  LGL A+ SDL+
Sbjct: 8   KKKVARQSSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLK 67

Query: 66  STVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEEEGGLTPMSQEESDLSE 125
           STV S++Q GN+GL F+DF++LH++LDD++FG      E + +        ++ ESDL+E
Sbjct: 68  STVESYIQPGNTGLNFDDFSSLHKTLDDSFFGGACGGGENEDDPSSA----AENESDLAE 127

Query: 126 AFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFFEFKDMM 185
           AF+VFDE+GDG+ISA ELQ VL+KL L EG E++RV+KMI+SVDRN DGRVDFFEFK+MM
Sbjct: 128 AFKVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMM 187

Query: 186 RSVIVKS 193
           R+V++ S
Sbjct: 188 RTVVIPS 190

[Close]

BLAST of Sed0006658 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 6.7e-54
Identity = 112/187 (59.89%), Postives = 141/187 (75.40%), Query Frame = 0


Query: 6   RARLMKKPSSFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAEQSDLE 65
           + +L ++ SSFRLRS SLN LRL R+FD+FDKN DG IT EEL QAL  LGL+A+ SDL+
Sbjct: 7   KKKLSRQSSSFRLRSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLK 66

Query: 66  STVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEEEGGLTPMSQEESDLSE 125
           STV SF++   +GLRF+DFAALH++LD+++FG E            G       ESDL E
Sbjct: 67  STVDSFIKPDKTGLRFDDFAALHKTLDESFFGGE------------GSCCDGSPESDLEE 126

Query: 126 AFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFFEFKDMM 185
           AF VFDEDGDG+ISA+ELQ VL+KL L E  EI++V+KMI+SVD NHDGRVDFFEFK+MM
Sbjct: 127 AFNVFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 181

Query: 186 RSVIVKS 193
           ++V+V S
Sbjct: 187 QTVVVPS 181

[Close]

BLAST of Sed0006658 vs. ExPASy Swiss-Prot
Match: Q09011 (Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 3.3e-53
Identity = 114/180 (63.33%), Postives = 142/180 (78.89%), Query Frame = 0


Query: 14  SSFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAEQSDLESTVSSFLQ 73
           SSFRLRS SLN++RLRRIFD+FD+N D +I+ EEL QAL LLGL+A+ S++ES V   ++
Sbjct: 27  SSFRLRSPSLNSIRLRRIFDVFDRNHDCLISVEELSQALNLLGLDADLSEIESMVKLHIK 86

Query: 74  TGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEEEGGLTP-MSQEESDLSEAFRVFDE 133
             N+GLRFEDF  LH+SL+D +FG + ED+        GL P  +Q+ESDL EAF VFDE
Sbjct: 87  PENTGLRFEDFETLHRSLNDVFFGSKCEDKL-------GLNPDPAQDESDLKEAFDVFDE 146

Query: 134 DGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFFEFKDMMRSVIVKS 193
           +GDG+ISA ELQVVL KL L EG+EIDRV+ MI SV+++HDGRVDFFEFKDMMR+VIV S
Sbjct: 147 NGDGFISAKELQVVLEKLGLPEGSEIDRVEMMISSVEQDHDGRVDFFEFKDMMRTVIVPS 199

[Close]

BLAST of Sed0006658 vs. ExPASy Swiss-Prot
Match: Q10LX4 (Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 GN=CML27 PE=2 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 2.1e-44
Identity = 101/183 (55.19%), Postives = 129/183 (70.49%), Query Frame = 0


Query: 9   LMKKPS-SFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAEQSDLEST 68
           L +KPS SFRLR+ SLN LRLRR+FD+FD+NGDG IT +E+  AL  LGL A+++ LE+T
Sbjct: 12  LSRKPSPSFRLRNGSLNALRLRRVFDLFDRNGDGEITLDEMASALDALGLGADRAGLEAT 71

Query: 69  VSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEEEGGLTPMSQEESDLSEAF 128
           V  ++  G +GLRF DF ALH++L D  FG   E+E  K  E+        +E D+ EAF
Sbjct: 72  VGGYIPAGAAGLRFGDFEALHRALGDALFGPVEEEEPGKQGED-------DDEGDMKEAF 131

Query: 129 RVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFFEFKDMMRS 188
           RVFDEDGDG+ISA ELQ VL+KL L E   +  VQ+MI +VDR+ DGRVDF EFK MM+ 
Sbjct: 132 RVFDEDGDGFISAAELQAVLKKLGLPEARNLATVQEMICNVDRDCDGRVDFGEFKCMMQG 187

Query: 189 VIV 191
           + V
Sbjct: 192 ITV 187

[Close]

BLAST of Sed0006658 vs. ExPASy TrEMBL
Match: A0A6J1J521 (calcium-binding allergen Bet v 3-like OS=Cucurbita maxima OX=3661 GN=LOC111481430 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 7.1e-75
Identity = 156/193 (80.83%), Postives = 171/193 (88.60%), Query Frame = 0


Query: 5   PRARLMKKPS----SFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAE 64
           PR RL KKPS    SFRLR  SLN+LRLRRIFDMFDKNGDGMITTEELGQALGLLGL+A+
Sbjct: 9   PRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDAD 68

Query: 65  QSDLESTVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEE-EGGLTPMSQE 124
           +SDL+STV+SF+  GN GLRFEDFAALHQSL+DTYFGE  EDEE+K E+ E  ++P+SQE
Sbjct: 69  ESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRETNMSPVSQE 128

Query: 125 ESDLSEAFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFF 184
           E DLSEAFRVFDEDGDGYISA ELQVVLRKLRL EGNE+DRVQKMILSVD N DGRVDFF
Sbjct: 129 ELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFF 188

Query: 185 EFKDMMRSVIVKS 193
           EFKDMMRSVIV+S
Sbjct: 189 EFKDMMRSVIVRS 201

[Close]

BLAST of Sed0006658 vs. ExPASy TrEMBL
Match: A0A0A0LA10 (Calcium binding protein/cast OS=Cucumis sativus OX=3659 GN=Csa_3G727960 PE=4 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 1.6e-74
Identity = 153/192 (79.69%), Postives = 170/192 (88.54%), Query Frame = 0


Query: 5   PRARLMKKP----SSFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAE 64
           PR RL KKP    SSFRLR  SLN+LRLRRIFDMFDKNGDGMITT+ELGQALGLLGL+A+
Sbjct: 9   PRPRLTKKPSSLSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTDELGQALGLLGLDAD 68

Query: 65  QSDLESTVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEEEGGLTPMSQEE 124
           +S+L+STV+SF++ GN GLRFEDFAALHQSL+DTYFG   EDE++  + E  ++ MSQEE
Sbjct: 69  ESELDSTVNSFIEPGNEGLRFEDFAALHQSLNDTYFGGMVEDEDEDEKRETSMSSMSQEE 128

Query: 125 SDLSEAFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFFE 184
           SDLSEAFRVFDEDGDGYISA ELQVVLRKLRL EGNEIDRVQKMILSVDRN DGRVDFFE
Sbjct: 129 SDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFE 188

Query: 185 FKDMMRSVIVKS 193
           FKDMMRSVIV+S
Sbjct: 189 FKDMMRSVIVRS 200

[Close]

BLAST of Sed0006658 vs. ExPASy TrEMBL
Match: A0A6J1F9I1 (calcium-binding allergen Bet v 3-like OS=Cucurbita moschata OX=3662 GN=LOC111442046 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 2.3e-73
Identity = 154/193 (79.79%), Postives = 169/193 (87.56%), Query Frame = 0


Query: 5   PRARLMKKPS----SFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAE 64
           PR RL KKPS    SFRLR  SLN+LRLRRIFDMFDKNGDGMITTEELGQALGLLGL+A+
Sbjct: 9   PRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDAD 68

Query: 65  QSDLESTVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEE-EGGLTPMSQE 124
           +SDL+STV+SF+  GN GLRFEDFAALHQ L+DTYF E  EDEE+K E+ E  ++P+SQE
Sbjct: 69  ESDLDSTVNSFIAPGNVGLRFEDFAALHQFLNDTYFREPVEDEEEKEEKRETNMSPVSQE 128

Query: 125 ESDLSEAFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFF 184
           E DLSEAFRVFDEDGDGYISA ELQVVLRKLRL EGNE+DRVQKMILSVD N DGRVDFF
Sbjct: 129 ELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFF 188

Query: 185 EFKDMMRSVIVKS 193
           EFKDMMRSVIV+S
Sbjct: 189 EFKDMMRSVIVRS 201

[Close]

BLAST of Sed0006658 vs. ExPASy TrEMBL
Match: A0A5A7TQ56 (Calcium-binding allergen Bet v 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00090 PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.5e-72
Identity = 153/192 (79.69%), Postives = 168/192 (87.50%), Query Frame = 0


Query: 5   PRARLMKKP----SSFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAE 64
           PR RL KKP    SSFRLR  SLN+LRLRRIFDMFDKNGDGMIT EELGQALGLLGL+A+
Sbjct: 9   PRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEELGQALGLLGLDAD 68

Query: 65  QSDLESTVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEEEGGLTPMSQEE 124
           +S+L+STV+SF++ GN GLRFEDFAALHQSL+DTYF    EDE++K   E  ++ MSQEE
Sbjct: 69  ESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEK--RETSMSSMSQEE 128

Query: 125 SDLSEAFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFFE 184
           SDLSEAFRVFDEDGDGYISA ELQVVLRKLRL EGNEIDRVQKMILSVDRN DGRVDFFE
Sbjct: 129 SDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFE 188

Query: 185 FKDMMRSVIVKS 193
           FKDMMRSVIV+S
Sbjct: 189 FKDMMRSVIVRS 198

[Close]

BLAST of Sed0006658 vs. ExPASy TrEMBL
Match: A0A1S3B620 (calcium-binding allergen Bet v 3 OS=Cucumis melo OX=3656 GN=LOC103486221 PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.5e-72
Identity = 153/192 (79.69%), Postives = 168/192 (87.50%), Query Frame = 0


Query: 5   PRARLMKKP----SSFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAE 64
           PR RL KKP    SSFRLR  SLN+LRLRRIFDMFDKNGDGMIT EELGQALGLLGL+A+
Sbjct: 9   PRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEELGQALGLLGLDAD 68

Query: 65  QSDLESTVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEEEGGLTPMSQEE 124
           +S+L+STV+SF++ GN GLRFEDFAALHQSL+DTYF    EDE++K   E  ++ MSQEE
Sbjct: 69  ESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEK--RETSMSSMSQEE 128

Query: 125 SDLSEAFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFFE 184
           SDLSEAFRVFDEDGDGYISA ELQVVLRKLRL EGNEIDRVQKMILSVDRN DGRVDFFE
Sbjct: 129 SDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFE 188

Query: 185 FKDMMRSVIVKS 193
           FKDMMRSVIV+S
Sbjct: 189 FKDMMRSVIVRS 198

[Close]

BLAST of Sed0006658 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 216.5 bits (550), Expect = 1.9e-56
Identity = 112/187 (59.89%), Postives = 148/187 (79.14%), Query Frame = 0


Query: 6   RARLMKKPSSFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAEQSDLE 65
           + ++ ++ SSFRLRS SLN LRL+RIFD+FDKNGDG IT EEL QAL  LGL A+ SDL+
Sbjct: 8   KKKVARQSSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLK 67

Query: 66  STVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEEEGGLTPMSQEESDLSE 125
           STV S++Q GN+GL F+DF++LH++LDD++FG      E + +        ++ ESDL+E
Sbjct: 68  STVESYIQPGNTGLNFDDFSSLHKTLDDSFFGGACGGGENEDDPSSA----AENESDLAE 127

Query: 126 AFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFFEFKDMM 185
           AF+VFDE+GDG+ISA ELQ VL+KL L EG E++RV+KMI+SVDRN DGRVDFFEFK+MM
Sbjct: 128 AFKVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMM 187

Query: 186 RSVIVKS 193
           R+V++ S
Sbjct: 188 RTVVIPS 190

[Close]

BLAST of Sed0006658 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 211.8 bits (538), Expect = 4.7e-55
Identity = 112/187 (59.89%), Postives = 141/187 (75.40%), Query Frame = 0


Query: 6   RARLMKKPSSFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAEQSDLE 65
           + +L ++ SSFRLRS SLN LRL R+FD+FDKN DG IT EEL QAL  LGL+A+ SDL+
Sbjct: 7   KKKLSRQSSSFRLRSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLK 66

Query: 66  STVSSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEEEGGLTPMSQEESDLSE 125
           STV SF++   +GLRF+DFAALH++LD+++FG E            G       ESDL E
Sbjct: 67  STVDSFIKPDKTGLRFDDFAALHKTLDESFFGGE------------GSCCDGSPESDLEE 126

Query: 126 AFRVFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFFEFKDMM 185
           AF VFDEDGDG+ISA+ELQ VL+KL L E  EI++V+KMI+SVD NHDGRVDFFEFK+MM
Sbjct: 127 AFNVFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 181

Query: 186 RSVIVKS 193
           ++V+V S
Sbjct: 187 QTVVVPS 181

[Close]

BLAST of Sed0006658 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 107.5 bits (267), Expect = 1.3e-23
Identity = 65/178 (36.52%), Postives = 98/178 (55.06%), Query Frame = 0


Query: 9   LMKKPSSFRLRSQSLNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAEQSDLESTV 68
           ++  PSS    ++ ++   L+R+F MFDKNGDG IT EEL  +L  LG+     DL   +
Sbjct: 47  MLPSPSSSSAPTKRIDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMI 106

Query: 69  SSFLQTGNSGLRFEDFAALHQSLDDTYFGEEAEDEEKKSEEEGGLTPMSQEESDLSEAFR 128
                 G+  +  ++F +L+ S+ D    E   D E              EE D+ +AF 
Sbjct: 107 HKIDANGDGCVDIDEFESLYSSIVD----EHHNDGE-------------TEEEDMKDAFN 166

Query: 129 VFDEDGDGYISALELQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFFEFKDMMR 187
           VFD+DGDG+I+  EL+ V+  L L +G  +D  +KMI+ VD + DGRV++ EF  MM+
Sbjct: 167 VFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEFLQMMK 207

[Close]

BLAST of Sed0006658 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 102.1 bits (253), Expect = 5.3e-22
Identity = 58/164 (35.37%), Postives = 94/164 (57.32%), Query Frame = 0


Query: 23  LNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAEQSDLESTVSSFLQTGNSGLRFE 82
           +++  L R+F MFDK+GDG ITT+EL ++   LG+   + +L   +      G+  +  E
Sbjct: 1   MDSTELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIE 60

Query: 83  DFAALHQSLDDTYFGEEAEDEEKKSEEEGGLTPMSQEESDLSEAFRVFDEDGDGYISALE 142
           +F  L++++         EDE++  EE            D+ EAF VFD +GDG+I+  E
Sbjct: 61  EFGELYKTI-------MVEDEDEVGEE------------DMKEAFNVFDRNGDGFITVDE 120

Query: 143 LQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFFEFKDMMR 187
           L+ VL  L L +G  ++  +KMI+ VD + DGRV++ EF+ MM+
Sbjct: 121 LKAVLSSLGLKQGKTLEECRKMIMQVDVDGDGRVNYMEFRQMMK 145

[Close]

BLAST of Sed0006658 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 101.3 bits (251), Expect = 9.0e-22
Identity = 60/164 (36.59%), Postives = 91/164 (55.49%), Query Frame = 0


Query: 23  LNTLRLRRIFDMFDKNGDGMITTEELGQALGLLGLEAEQSDLESTVSSFLQTGNSGLRFE 82
           ++   L+R+F MFDKNGDG IT +EL + L  LG+     +L   +      G+  +  +
Sbjct: 1   MDPTELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDID 60

Query: 83  DFAALHQSLDDTYFGEEAEDEEKKSEEEGGLTPMSQEESDLSEAFRVFDEDGDGYISALE 142
           +F  L++++ D       EDE               EE D+ EAF VFD++GDG+I+  E
Sbjct: 61  EFGELYKTIMD------EEDE---------------EEEDMKEAFNVFDQNGDGFITVDE 120

Query: 143 LQVVLRKLRLDEGNEIDRVQKMILSVDRNHDGRVDFFEFKDMMR 187
           L+ VL  L L +G  +D  +KMI  VD + DGRV++ EF+ MM+
Sbjct: 121 LKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

The following BLAST results are available for this feature:

BLAST of Sed0006658 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023527056.1	1.1e-74	80.83	calcium-binding allergen Bet v 3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022982604.1	1.5e-74	80.83	calcium-binding allergen Bet v 3-like [Cucurbita maxima]	[more]
XP_004137668.1	3.3e-74	79.69	calcium-binding protein CML42 [Cucumis sativus] >KGN58678.1 hypothetical protein...	[more]
KAG6580420.1	9.5e-74	80.31	Calcium-binding allergen Bet v 3, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022935065.1	4.7e-73	79.79	calcium-binding allergen Bet v 3-like [Cucurbita moschata]	[more]

BLAST of Sed0006658 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43187	4.0e-59	66.49	Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1	[more]
Q9SVG9	2.7e-55	59.89	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
Q9FI19	6.7e-54	59.89	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]
Q09011	3.3e-53	63.33	Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]
Q10LX4	2.1e-44	55.19	Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Sed0006658 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J521	7.1e-75	80.83	calcium-binding allergen Bet v 3-like OS=Cucurbita maxima OX=3661 GN=LOC11148143...	[more]
A0A0A0LA10	1.6e-74	79.69	Calcium binding protein/cast OS=Cucumis sativus OX=3659 GN=Csa_3G727960 PE=4 SV=...	[more]
A0A6J1F9I1	2.3e-73	79.79	calcium-binding allergen Bet v 3-like OS=Cucurbita moschata OX=3662 GN=LOC111442...	[more]
A0A5A7TQ56	1.5e-72	79.69	Calcium-binding allergen Bet v 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B620	1.5e-72	79.69	calcium-binding allergen Bet v 3 OS=Cucumis melo OX=3656 GN=LOC103486221 PE=4 SV...	[more]

BLAST of Sed0006658 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20780.1	1.9e-56	59.89	calmodulin like 42	[more]
AT5G44460.1	4.7e-55	59.89	calmodulin like 43	[more]
AT2G43290.1	1.3e-23	36.52	Calcium-binding EF-hand family protein	[more]
AT4G03290.1	5.3e-22	35.37	EF hand calcium-binding protein family	[more]
AT1G05990.1	9.0e-22	36.59	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 160..188 e-value: 3.6E-6 score: 36.5 coord: 122..150 e-value: 6.7E-6 score: 35.6 coord: 27..55 e-value: 2.4E-6 score: 37.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 121..186 e-value: 1.1E-12 score: 48.2
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 27..56 e-value: 1.7E-8 score: 33.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 156..191 score: 14.06708
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 118..153 score: 14.987627
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 23..58 score: 15.712908
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 122..186 e-value: 8.10487E-16 score: 66.8025
None	No IPR available	GENE3D	1.10.238.10		coord: 153..193 e-value: 5.3E-6 score: 28.3
None	No IPR available	GENE3D	1.10.238.10		coord: 114..152 e-value: 9.3E-11 score: 43.1
None	No IPR available	GENE3D	1.10.238.10		coord: 25..93 e-value: 5.1E-13 score: 50.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 97..122
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 10..192
None	No IPR available	PANTHER	PTHR10891:SF743	CALCIUM-BINDING PROTEIN CML42	coord: 10..192
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 169..181
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 36..48
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 131..143
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 27..186

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0006658.1	Sed0006658.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding