Sed0006414 (gene) Chayote v1

BLAST of Sed0006414 vs. NCBI nr
Match: XP_022990941.1 (subtilisin-like protease SBT1.1 [Cucurbita maxima] >XP_022990948.1 subtilisin-like protease SBT1.1 [Cucurbita maxima])

HSP 1 Score: 1263.8 bits (3269), Expect = 0.0e+00
Identity = 636/767 (82.92%), Postives = 690/767 (89.96%), Query Frame = 0

Query: 5   MGFRGIWVLFSIMLVTSSNAALDEQTYIIHMDTTKMATTNPEQWYTSIIDSVNQLASLDD 64
           MGFR + +  SI L TS+ AA+D+Q+YIIHMDTTKMA  +PEQWYT++IDS+N+++SLDD
Sbjct: 1   MGFREVCLFLSIFLATSA-AAVDQQSYIIHMDTTKMAAPSPEQWYTALIDSINKISSLDD 60

Query: 65  DEDEKSNVAEILYIYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKFL 124
            E E S+ A+ILY+YKTAISGFAAKLS KKL+SLSK PGFLAATPNELLQLHTTHSP+FL
Sbjct: 61  QE-EASSAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFL 120

Query: 125 GLQNSHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSN 184
           GLQ  HGLWNSSNLASDI+IG+LDTGIWPEHISFQDKGLPPVPK+WKG CQAG KFS SN
Sbjct: 121 GLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSN 180

Query: 185 CNNKLVGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGM 244
           CN KL+GA+AYIKGYEAIVGRLN TGTFRSPRDSDGHGTHTASTAAGN+V  AS F+Q M
Sbjct: 181 CNRKLIGATAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAM 240

Query: 245 GVATGMRFTSRIAAYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIA 304
           GVATGMRFTSRIAAYKVCW EGCANADILAAIDRAVADGVDVLSLSLGG AS FYKD+IA
Sbjct: 241 GVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIA 300

Query: 305 IATFGAVQKGVFVSCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEG 364
           IATFGAV+ GVFVSCSAGNSGP SSTV N APWIMTVAASYTDRTFPA VKLGNG VFEG
Sbjct: 301 IATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFEG 360

Query: 365 SSLYYGNNTIQLPLVYNKTAGGNDANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEVVK 424
           SSLY GN+  QLPLVYN TAGG +ANVC  GSLVPSLVKGKIV+CERG+NSRTAKGE VK
Sbjct: 361 SSLYSGNSIGQLPLVYNNTAGGEEANVCTAGSLVPSLVKGKIVVCERGTNSRTAKGEQVK 420

Query: 425 SAGGAGMILINTQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFEGT 484
            AGGAGMILINTQLEGEELFADPHVLPA NLGASAG+AII YI SSSK+QPK  I FEGT
Sbjct: 421 LAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYI-SSSKHQPKALIAFEGT 480

Query: 485 KYGSRAPRVAAFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIFNI 544
           K+G+RAPRVAAFSSRGPS +  DVIKPDVTAPGVNILAAWPL  SP+ELESDKRRV+FNI
Sbjct: 481 KFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNI 540

Query: 545 ISGTSMSCPHASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGKPAD 604
           ISGTSMSCPH SGLAALLKSAHK WSPAAIKSALMTTAYT DNR  PISDVGSESGKPA+
Sbjct: 541 ISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSESGKPAN 600

Query: 605 PFAFGSGHVDPEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTCPSKKQILQ 664
           PFAFGSGHVDPEKASDPGLIYDIT QDYLNY CSL YN+TQI L+SRGNFTCPSK+++ Q
Sbjct: 601 PFAFGSGHVDPEKASDPGLIYDITPQDYLNYFCSLNYNSTQIGLVSRGNFTCPSKRRVGQ 660

Query: 665 PGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLNFG 724
           PG+LNYPSFSVFMKKKAKNVSVT KRTVTNVG P SDYTVKI NPKGI I V+P KL+F 
Sbjct: 661 PGNLNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYTVKIKNPKGIGIRVEPEKLSFR 720

Query: 725 KLGEKLSYKVSFVSLGKREALGKFSFGSIVWISGKYAVRSPIAVTWQ 772
           + G+KLSY+VSFV+LGKRE LG FSFGS+VW+SGKY VRSPIAVTW+
Sbjct: 721 RYGQKLSYQVSFVALGKREGLGGFSFGSLVWVSGKYGVRSPIAVTWK 764

BLAST of Sed0006414 vs. NCBI nr
Match: XP_038893069.1 (subtilisin-like protease SBT1.1 [Benincasa hispida] >XP_038893070.1 subtilisin-like protease SBT1.1 [Benincasa hispida])

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 631/769 (82.05%), Postives = 689/769 (89.60%), Query Frame = 0

Query: 5   MGFRGIWVLFSIMLVTSSNAALDEQTYIIHMDTTKMATTNPEQWYTSIIDSVNQLASLDD 64
           MGFR +W+  SIML  ++ AA+D+QTYIIHMDTTKM TTNPEQWYT++IDS+N+L SLDD
Sbjct: 1   MGFREVWMFLSIMLAIAT-AAVDQQTYIIHMDTTKMTTTNPEQWYTAMIDSLNELPSLDD 60

Query: 65  D-EDEKSNVAEILYIYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKF 124
           + ++E S+ AEILY+YKTA+SGFAAKLS KKL+SLSKIPGFLAATPNELLQLHTTHSP+F
Sbjct: 61  ENKEEASDTAEILYVYKTALSGFAAKLSRKKLDSLSKIPGFLAATPNELLQLHTTHSPQF 120

Query: 125 LGLQNSHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRS 184
           LGL+  HGLWNSSNLASDIIIG+LDTGIWPEHISFQDKGL PVP +WKGICQAGPKFS S
Sbjct: 121 LGLERDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPSKWKGICQAGPKFSPS 180

Query: 185 NCNNKLVGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQG 244
           NCN KL+GASAYIKGYEAIVG LNETGTFRSPRDSDGHGTHTASTAAG++V  AS F+QG
Sbjct: 181 NCNKKLIGASAYIKGYEAIVGTLNETGTFRSPRDSDGHGTHTASTAAGSMVNKASFFNQG 240

Query: 245 MGVATGMRFTSRIAAYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEI 304
           MGVATGM +TSRIAAYKVCWP GCANADILAA+D AVADGVDVLSLSLGGGA  FY+D I
Sbjct: 241 MGVATGMMYTSRIAAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNI 300

Query: 305 AIATFGAVQKGVFVSCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFE 364
           AIA FGA+Q GVFVSCSAGNSGPF STVGNAAPWIMTVAASYTDRTFP  VKLGNG VFE
Sbjct: 301 AIAAFGAIQNGVFVSCSAGNSGPFISTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFE 360

Query: 365 GSSLYYGNNTIQLPLVYNKTAG-GNDANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEV 424
           GSSLYYG N   LPLVYN TAG G + N C  GSL P++VKGKIV+CERGSNSRT KGE 
Sbjct: 361 GSSLYYGKNINYLPLVYNNTAGDGQETNFCTAGSLDPTMVKGKIVLCERGSNSRTDKGEQ 420

Query: 425 VKSAGGAGMILINTQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFE 484
           VK AGGAGMILINTQ EGEELFADPHVLPAT LGASAGKAI++YI +SSK+Q K S+ FE
Sbjct: 421 VKLAGGAGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYI-ASSKSQAKASMAFE 480

Query: 485 GTKYGSRAPRVAAFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIF 544
           GTKYGSRAPRVAAFSSRGPS VG DV+KPDVTAPGVNILAAWP  VSP+EL+SDKRRV+F
Sbjct: 481 GTKYGSRAPRVAAFSSRGPSFVGPDVMKPDVTAPGVNILAAWPPIVSPSELDSDKRRVLF 540

Query: 545 NIISGTSMSCPHASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGKP 604
           NIISGTSMSCPH SGLAALLKSAHK WSPAAIKSALMTTAY TDN+   +SDVG  SG P
Sbjct: 541 NIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKMNLVSDVGHPSGGP 600

Query: 605 ADPFAFGSGHVDPEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTCPSKKQI 664
           ADPFAFGSGHVDPEKASDPGL+YDIT QDY+NYLCSLKYN+TQIAL+SRGNFTC SK+ +
Sbjct: 601 ADPFAFGSGHVDPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTL 660

Query: 665 LQPGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLN 724
           LQP DLNYPSFSVFMKKKAKNVS+T KRTVTNVGIP SDYTVKINNPKGI ITVKP KL+
Sbjct: 661 LQPRDLNYPSFSVFMKKKAKNVSITSKRTVTNVGIPRSDYTVKINNPKGIRITVKPEKLS 720

Query: 725 FGKLGEKLSYKVSFVSLGKREALGKFSFGSIVWISGKYAVRSPIAVTWQ 772
           FG LGEKLS++VSFV+LG +EAL KFSFG +VW+SGKYAVRSPIAVTWQ
Sbjct: 721 FGSLGEKLSFQVSFVALGGKEALSKFSFGDLVWLSGKYAVRSPIAVTWQ 767

BLAST of Sed0006414 vs. NCBI nr
Match: XP_023544740.1 (subtilisin-like protease SBT1.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1256.5 bits (3250), Expect = 0.0e+00
Identity = 631/767 (82.27%), Postives = 687/767 (89.57%), Query Frame = 0

Query: 5   MGFRGIWVLFSIMLVTSSNAALDEQTYIIHMDTTKMATTNPEQWYTSIIDSVNQLASLDD 64
           MGFR + +  SI L TS+ AA+D+Q+YIIHMDTTKMA   PEQWYT++IDS+N+++SL  
Sbjct: 1   MGFREVCLFLSIFLATST-AAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSL-H 60

Query: 65  DEDEKSNVAEILYIYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKFL 124
           D+ E SN A+ILY+YKTAISGFAAKLS KKL+SLSK PGFLAATPNELLQLHTTHSP+FL
Sbjct: 61  DQQEASNAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFL 120

Query: 125 GLQNSHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSN 184
           GLQ  HGLWNSSNLASDI+IG+LDTGIWPEHISFQDKGLPPVPK+WKG CQ G KFS SN
Sbjct: 121 GLQRQHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQPGQKFSPSN 180

Query: 185 CNNKLVGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGM 244
           CN KL+GA AYIKGYEAIVGRLN TGTFRSPRDSDGHGTHTASTAAGN+V  AS F+Q M
Sbjct: 181 CNRKLIGAMAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAM 240

Query: 245 GVATGMRFTSRIAAYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIA 304
           GVATGMRFTSRIAAYKVCW EGCANADILAAIDRAVADGVDVLSLSLGG AS FYKD+IA
Sbjct: 241 GVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIA 300

Query: 305 IATFGAVQKGVFVSCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEG 364
           IATFGAV+ GVFVSCSAGNSGP SSTV N APWIMTVAASYTDRTFPA VKLGNG VFEG
Sbjct: 301 IATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFEG 360

Query: 365 SSLYYGNNTIQLPLVYNKTAGGNDANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEVVK 424
           SSLY GNN  QLPLVYN TAGG +ANVC PGSLVPS+VKGKIV+CERG+NSRTAKGE VK
Sbjct: 361 SSLYTGNNIGQLPLVYNNTAGGQEANVCTPGSLVPSMVKGKIVVCERGTNSRTAKGEQVK 420

Query: 425 SAGGAGMILINTQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFEGT 484
            AGGAGMILINTQLEGEELFADPHVLPA NLGASAG+AII YI SSSK+QPK  I FEGT
Sbjct: 421 LAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYI-SSSKHQPKALIGFEGT 480

Query: 485 KYGSRAPRVAAFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIFNI 544
           K+G+RAPRVAAFSSRGPS +  DVIKPDVTAPGVNILAAWPL  SP+ELESDKRRV+FN+
Sbjct: 481 KFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNV 540

Query: 545 ISGTSMSCPHASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGKPAD 604
           ISGTSMSCPH SGLAALLKSAHK WSPAAIKSALMTTAYT DNR  PISDVGS SGKPA+
Sbjct: 541 ISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPAN 600

Query: 605 PFAFGSGHVDPEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTCPSKKQILQ 664
           PFAFGSGHVDPEKASDPGLIYDIT QDYL+Y CSL YN+TQI L+SRGNFTCPSK+++ Q
Sbjct: 601 PFAFGSGHVDPEKASDPGLIYDITPQDYLDYFCSLNYNSTQIGLVSRGNFTCPSKRRVGQ 660

Query: 665 PGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLNFG 724
           PG+LNYPSFSVFMKKKAKNVSVT KRTVTNVG P SDY+VKI NPKGI I+VKP KL+F 
Sbjct: 661 PGNLNYPSFSVFMKKKAKNVSVTLKRTVTNVGGPRSDYSVKIKNPKGIVISVKPEKLSFR 720

Query: 725 KLGEKLSYKVSFVSLGKREALGKFSFGSIVWISGKYAVRSPIAVTWQ 772
           + G+KLSY+VSFV+LGKRE +  FSFGS+VW+SGKYAVRSPIAVTW+
Sbjct: 721 RYGQKLSYQVSFVALGKREGVSGFSFGSLVWVSGKYAVRSPIAVTWK 764

BLAST of Sed0006414 vs. NCBI nr
Match: XP_022941552.1 (subtilisin-like protease SBT1.1 [Cucurbita moschata] >XP_022941553.1 subtilisin-like protease SBT1.1 [Cucurbita moschata])

HSP 1 Score: 1254.6 bits (3245), Expect = 0.0e+00
Identity = 631/767 (82.27%), Postives = 686/767 (89.44%), Query Frame = 0

Query: 5   MGFRGIWVLFSIMLVTSSNAALDEQTYIIHMDTTKMATTNPEQWYTSIIDSVNQLASLDD 64
           MGFR + +  SI L TS+ AA+D+Q+YIIHMDTTKMA   PEQWYT++IDS+N+++SL+D
Sbjct: 1   MGFREVCLFLSIFLATST-AAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSLED 60

Query: 65  DEDEKSNVAEILYIYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKFL 124
            E E SN A+ILY+YKTAISGFAAKLS KKL+SLSK PGFLAATPNELLQLHTTHSP+FL
Sbjct: 61  QE-EASNAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFL 120

Query: 125 GLQNSHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSN 184
           GLQ  HGLWNSSNLASDI+IG+LDTGIWPEHISFQDKGLPPVPK+WKG CQAG KFS SN
Sbjct: 121 GLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSN 180

Query: 185 CNNKLVGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGM 244
           CN KL+GA AYIKGYEAIVGRLN TGTFRSPRDSDGHGTHTASTAAGN+V  AS F+Q M
Sbjct: 181 CNRKLIGAIAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAM 240

Query: 245 GVATGMRFTSRIAAYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIA 304
           GVATGMRFTSRIAAYKVCW EGCANADILAAIDRAVADGVDVLSLSLGG AS FYKD+IA
Sbjct: 241 GVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIA 300

Query: 305 IATFGAVQKGVFVSCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEG 364
           IATFGAV+ GVFVSCSAGNSGP SSTV N APWIMTVAASYTDR+FPA VKLGNG VFEG
Sbjct: 301 IATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRSFPATVKLGNGQVFEG 360

Query: 365 SSLYYGNNTIQLPLVYNKTAGGNDANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEVVK 424
           SSLY GNN  QLPLVYN TAGG DANVC  GSLVPS+VKGKIV+CERG+NSRTAKGE VK
Sbjct: 361 SSLYSGNNIGQLPLVYNNTAGGEDANVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQVK 420

Query: 425 SAGGAGMILINTQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFEGT 484
            AGGAGMILINTQLEGEELFADPHVLPA NLGASAG+AII YI SSSK+QPK  I FEGT
Sbjct: 421 LAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYI-SSSKHQPKALIAFEGT 480

Query: 485 KYGSRAPRVAAFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIFNI 544
           K+G+RAPRVAAFSSRGPS +  DVIKPDVTAPGVNILAAWPL  SP+E+ESDKRRV+FN+
Sbjct: 481 KFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSEVESDKRRVLFNV 540

Query: 545 ISGTSMSCPHASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGKPAD 604
           ISGTSMSCPH SGLAALLKSAHK WSPAAIKSALMTTAYT DNR  PISDVGS SGKPA+
Sbjct: 541 ISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPAN 600

Query: 605 PFAFGSGHVDPEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTCPSKKQILQ 664
           PFAFGSGHVDPEKASDPGLIYDIT QDYLNYLCSL YN+TQI L+SRGNFTCPSK+++ Q
Sbjct: 601 PFAFGSGHVDPEKASDPGLIYDITPQDYLNYLCSLNYNSTQIGLVSRGNFTCPSKRRVGQ 660

Query: 665 PGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLNFG 724
            G LNYPSFSVFMKKKAKNVSVT KRTVTNVG P SDY+VKI NPKGI I+VKP KL+F 
Sbjct: 661 AGKLNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYSVKIKNPKGIGISVKPEKLSFR 720

Query: 725 KLGEKLSYKVSFVSLGKREALGKFSFGSIVWISGKYAVRSPIAVTWQ 772
           + G+KLSY+VSFV+LGKRE +  FSFGS+VW+SG YAVRSPIAVTW+
Sbjct: 721 RYGQKLSYQVSFVALGKREGVSGFSFGSLVWVSGTYAVRSPIAVTWK 764

BLAST of Sed0006414 vs. NCBI nr
Match: XP_008454762.1 (PREDICTED: subtilisin-like protease SBT1.1 [Cucumis melo] >XP_016901624.1 PREDICTED: subtilisin-like protease SBT1.1 [Cucumis melo])

HSP 1 Score: 1236.1 bits (3197), Expect = 0.0e+00
Identity = 627/768 (81.64%), Postives = 681/768 (88.67%), Query Frame = 0

Query: 5   MGFRGIWVLFSIMLVTSSNAALDEQTYIIHMDTTKMATTNPEQWYTSIIDSVNQLASLDD 64
           MGFR +WVL SIML  SS A +D+QTYIIHMDTTKM T NPEQWYT IIDSVN+L+SLDD
Sbjct: 1   MGFREVWVL-SIMLAISS-AVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSLDD 60

Query: 65  DEDEKSNVAEILYIYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKFL 124
           +E E SN AEILY+YKTA+SGFAAKL++KKL+SLSKIPGFLAATPNELLQLHTTHSP+FL
Sbjct: 61  NE-EASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFL 120

Query: 125 GLQNSHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSN 184
           GLQ  HGLWN SNLASDIIIG+LDTGIWPEHISFQDKGL  VP +WKGICQ GP+FS SN
Sbjct: 121 GLQRDHGLWNFSNLASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSSN 180

Query: 185 CNNKLVGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGM 244
           CN KL+GASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAG++V NAS ++QGM
Sbjct: 181 CNKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQGM 240

Query: 245 GVATGMRFTSRIAAYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIA 304
           GVA+GMRFTSRI AYKVCWP GCANADILAA+D AVADGVDVLSLSLGGG+S FYKD IA
Sbjct: 241 GVASGMRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIA 300

Query: 305 IATFGAVQKGVFVSCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEG 364
           IA FGA+QKGVFVSCSAGNSGP  STVGNAAPWIMTVAASYTDRTFP  VKLGNG VFEG
Sbjct: 301 IAAFGAIQKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEG 360

Query: 365 SSLYYGNNTIQLPLVYNKTAG-GNDANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEVV 424
           SSLYYG +  +LPLVYN TAG G + NVCI GSL PS+VKGKIVICERG+ SRT KGE V
Sbjct: 361 SSLYYGKSINELPLVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQV 420

Query: 425 KSAGGAGMILINTQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFEG 484
           K AGG GMILINTQ EGEELFADPHVLPAT LGASAGKAI++YI +SSK Q K SI FEG
Sbjct: 421 KLAGGTGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYI-ASSKTQAKASIVFEG 480

Query: 485 TKYGSRAPRVAAFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIFN 544
           TKYGS+APRVAAFSSRGPS VG DVIKPDVTAPGVNILAAWP  VSP+EL SD RRV+FN
Sbjct: 481 TKYGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELASDTRRVMFN 540

Query: 545 IISGTSMSCPHASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGKPA 604
           IISGTSMSCPH SGLAALLKSAH  WSPAAIKSALMTTAY TD++   ISDVG  +G+PA
Sbjct: 541 IISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPA 600

Query: 605 DPFAFGSGHVDPEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTCPSKKQIL 664
            PF FGSGHVDPEKASDPGLIYDIT QDY+NYLCSLKYN++QIAL+SRGN TC SK+ ++
Sbjct: 601 TPFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVV 660

Query: 665 QPGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLNF 724
           +PGDLNYPSFSVFMKKKAK VS+T KRTVTNVGI  SDYTVKINNPKG+ + VKP KL+F
Sbjct: 661 KPGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLSF 720

Query: 725 GKLGEKLSYKVSFVSLGKREALGKFSFGSIVWISGKYAVRSPIAVTWQ 772
           G LGE+LSYKVSFVSLG +EAL KFSFGS+VWISGKYAVRSPI VTWQ
Sbjct: 721 GSLGEQLSYKVSFVSLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 764

BLAST of Sed0006414 vs. ExPASy Swiss-Prot
Match: Q84WS0 (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana OX=3702 GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 3.2e-240
Identity = 429/769 (55.79%), Postives = 554/769 (72.04%), Query Frame = 0

Query: 5   MGFRGIWVLFSIMLVTSSNAALDEQTYIIHMDTTKMATTNPEQWYTSIIDSVNQLASLDD 64
           M F   +++F  ++  +SN +  +QTY+IH       TT+ +   TS+ +S+ Q  +++D
Sbjct: 18  MMFFRSFIVFFFLIFFASNVSSRKQTYVIH-----TVTTSTKHIVTSLFNSL-QTENIND 77

Query: 65  DEDEKSNVAEILYIYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKFL 124
           D+    ++ EI YIY+ A+SGF+A L++ +L+++    GF++A P+ELL LHTT+S +FL
Sbjct: 78  DD---FSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFL 137

Query: 125 GLQNSHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSN 184
           GL+   GLWN ++L+SD+IIG++DTGI PEH+SF+D  + PVP RW+G C  G  FS S 
Sbjct: 138 GLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSE 197

Query: 185 CNNKLVGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGM 244
           CN K++GASA+ KGYE+IVG++NET  FRS RD+ GHGTHTASTAAG++V  A+ F Q  
Sbjct: 198 CNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAK 257

Query: 245 GVATGMRFTSRIAAYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIA 304
           G+A+GMRFTSRIAAYK CW  GCA+ D++AAIDRA+ DGVDV+SLSLGG +  FY D IA
Sbjct: 258 GLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIA 317

Query: 305 IATFGAVQKGVFVSCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEG 364
           IA FGA+QK +FVSCSAGNSGP +STV N APW+MTVAASYTDRTFPAIV++GN     G
Sbjct: 318 IAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVG 377

Query: 365 SSLYYGNNTIQLPLVYNKTAGGNDANV-CIPGSLVPSLVKGKIVICERGSNSRTAKGEVV 424
           SSLY G +   LPL +N+TAG     V CI  SL   LV+GKIVIC RG++ RTAKGE V
Sbjct: 378 SSLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEV 437

Query: 425 KSAGGAGMILINTQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFEG 484
           K +GGA M+L++T+ EGEEL ADPHVLPA +LG S GK ++NY+  ++      S+ F G
Sbjct: 438 KRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAA--NATASVRFRG 497

Query: 485 TKYGSRAPRVAAFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIFN 544
           T YG+ AP VAAFSSRGPS  G ++ KPD+ APG+NILA W    SP+ L SD RRV FN
Sbjct: 498 TAYGATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFN 557

Query: 545 IISGTSMSCPHASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGK-P 604
           IISGTSM+CPH SG+AAL+KS H  WSPA IKSA+MTTA  TDNR RPI D G+   +  
Sbjct: 558 IISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESA 617

Query: 605 ADPFAFGSGHVDPEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTCPSKKQI 664
           A  FAFG+G+VDP +A DPGL+YD +  DYLNYLCSL Y + +I L S  N+TC S   +
Sbjct: 618 ATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVV 677

Query: 665 LQPGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLN 724
           L PGDLNYPSF+V +   A   +V +KRTVTNVG P  +Y V +  PKG+++ V+P  L 
Sbjct: 678 LSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLK 737

Query: 725 FGKLGEKLSYKVSFVSLGKREALGKFSFGSIVWISGKYAVRSPIAVTWQ 772
           F K  E+LSY V++ +   R +    SFG +VWI  KY VRSPIAVTW+
Sbjct: 738 FQKARERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Sed0006414 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 1.2e-199
Identity = 374/758 (49.34%), Postives = 506/758 (66.75%), Query Frame = 0

Query: 22  SNAALDEQTYIIHMDTTKMATTNP--EQWYTSIIDSVNQLASLDDDEDEKSNVAEILYIY 81
           S+++ D+ TYI+HM  ++M ++      WY S + S+             S+ AE+LY Y
Sbjct: 23  SSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI-------------SDSAELLYTY 82

Query: 82  KTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKFLGL-QNSHGLWNSSNL 141
           + AI GF+ +L+ ++ +SL   PG ++  P    +LHTT +P FLGL +++  L+  +  
Sbjct: 83  ENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGS 142

Query: 142 ASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSNCNNKLVGASAYIKG 201
            SD+++GVLDTG+WPE  S+ D+G  P+P  WKG C+AG  F+ S CN KL+GA  + +G
Sbjct: 143 YSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARG 202

Query: 202 YEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGMGVATGMRFTSRIAA 261
           YE+ +G ++E+   RSPRD DGHGTHT+STAAG+VV  ASL     G A GM   +R+A 
Sbjct: 203 YESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAV 262

Query: 262 YKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIAIATFGAVQKGVFVS 321
           YKVCW  GC ++DILAAID+A+AD V+VLS+SLGGG S +Y+D +AI  F A+++G+ VS
Sbjct: 263 YKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVS 322

Query: 322 CSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEGSSLYYGNNTIQ--L 381
           CSAGN+GP SS++ N APWI TV A   DR FPA+  LGNG  F G SL+ G       L
Sbjct: 323 CSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLL 382

Query: 382 PLVY-NKTAGGNDANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEVVKSAGGAGMILIN 441
           P +Y    +   + N+C+ G+L+P  VKGKIV+C+RG N+R  KG+VVK+AGG GMIL N
Sbjct: 383 PFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILAN 442

Query: 442 TQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFEGTKYGSR-APRVA 501
           T   GEEL AD H+LPAT +G  AG  I +Y+T+     P  SI+  GT  G + +P VA
Sbjct: 443 TAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDP--NPTASISILGTVVGVKPSPVVA 502

Query: 502 AFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIFNIISGTSMSCPH 561
           AFSSRGP+ +  +++KPD+ APGVNILAAW     PT L SD RRV FNIISGTSMSCPH
Sbjct: 503 AFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPH 562

Query: 562 ASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGKPADPFAFGSGHVD 621
            SGLAALLKS H  WSPAAI+SALMTTAY T    +P+ D+   +GKP+ PF  G+GHV 
Sbjct: 563 VSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA--TGKPSTPFDHGAGHVS 622

Query: 622 PEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTC-PSKKQILQPGDLNYPSF 681
           P  A++PGLIYD+T +DYL +LC+L Y + QI  +SR N+TC PSK   +   DLNYPSF
Sbjct: 623 PTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSV--ADLNYPSF 682

Query: 682 SVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKI-NNPKGIEITVKPMKLNFGKLGEKLSY 741
           +V +       +  + RTVT+VG     Y+VK+ +   G++I+V+P  LNF +  EK SY
Sbjct: 683 AVNVDGVG---AYKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSY 742

Query: 742 KVSFVSLGKREALGKFSFGSIVWISGKYAVRSPIAVTW 771
            V+F ++   +  G  SFGSI W  GK+ V SP+A++W
Sbjct: 743 TVTF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Sed0006414 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 1.0e-198
Identity = 385/777 (49.55%), Postives = 501/777 (64.48%), Query Frame = 0

Query: 13  LFSIMLVTSSNAALDEQTYIIHMDTTKMATTNPE--QWYTSIIDSVNQLASLDDDEDEKS 72
           L ++   +SS ++ +  TYI+H+D     +  P    WYTS       LASL       S
Sbjct: 10  LLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTS------SLASL------TS 69

Query: 73  NVAEILYIYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKFLGLQNSH 132
           +   I++ Y T   GF+A+L+++  + L   P  ++  P ++  LHTT SP+FLGL+++ 
Sbjct: 70  SPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTD 129

Query: 133 --GLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSNCNNK 192
             GL   S+  SD++IGV+DTG+WPE  SF D+GL PVP +WKG C A   F  S CN K
Sbjct: 130 KAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRK 189

Query: 193 LVGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGMGVAT 252
           LVGA  +  GYEA  G++NET  FRSPRDSDGHGTHTAS +AG  V+ AS      GVA 
Sbjct: 190 LVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAA 249

Query: 253 GMRFTSRIAAYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIAIATF 312
           GM   +R+AAYKVCW  GC ++DILAA D AVADGVDV+SLS+GG    +Y D IAI  F
Sbjct: 250 GMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAF 309

Query: 313 GAVQKGVFVSCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEGSSLY 372
           GA+ +G+FVS SAGN GP + TV N APW+ TV A   DR FPA VKLGNG +  G S+Y
Sbjct: 310 GAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVY 369

Query: 373 YG---NNTIQLPLVYNKTAGGND---ANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEV 432
            G   +     PLVY  +  G D   +++C+ GSL P+LVKGKIV+C+RG NSR  KGE+
Sbjct: 370 GGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEI 429

Query: 433 VKSAGGAGMILINTQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQ----PKGS 492
           V+  GG GMI+ N   +GE L AD HVLPAT++GAS G  I  YI+ SSK++    P  +
Sbjct: 430 VRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTAT 489

Query: 493 ITFEGTKYGSR-APRVAAFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDK 552
           I F+GT+ G R AP VA+FS+RGP+    +++KPDV APG+NILAAWP  + P+ + SD 
Sbjct: 490 IVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDN 549

Query: 553 RRVIFNIISGTSMSCPHASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGS 612
           RR  FNI+SGTSM+CPH SGLAALLK+AH  WSPAAI+SAL+TTAYT DN   P+ D   
Sbjct: 550 RRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD--E 609

Query: 613 ESGKPADPFAFGSGHVDPEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTCP 672
            +G  +    +GSGHV P KA DPGL+YDIT+ DY+N+LC+  Y  T I  I+R    C 
Sbjct: 610 STGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCD 669

Query: 673 SKKQILQPGDLNYPSFS-VFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITV 732
             ++    G+LNYPSFS VF +     +S  F RTVTNVG   S Y +KI  P+G  +TV
Sbjct: 670 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTV 729

Query: 733 KPMKLNFGKLGEKLSYKVSFVSLGKREALG--KFSFGSIVWISGKYAVRSPIAVTWQ 772
           +P KL+F ++G+KLS+ V   +   + + G      G IVW  GK  V SP+ VT Q
Sbjct: 730 EPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of Sed0006414 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 6.9e-195
Identity = 368/777 (47.36%), Postives = 500/777 (64.35%), Query Frame = 0

Query: 10  IWVLFSIMLV----TSSNAALDEQTYIIHMDTTKMAT--TNPEQWYTSIIDSVNQLASLD 69
           ++++ SI L+     ++     ++TY+IHMD + M    TN  QWY+S I+SV Q  S  
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS-- 71

Query: 70  DDEDEKSNVAEILYIYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKF 129
             ++E+ N   ILY Y+TA  G AA+L+ ++   L +  G +A  P    +LHTT SP F
Sbjct: 72  --QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTF 131

Query: 130 LGL--QNSHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFS 189
           LGL  Q S  +W       D+++GVLDTGIWPE  SF D G+ PVP  W+G C+ G +F 
Sbjct: 132 LGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFL 191

Query: 190 RSNCNNKLVGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFS 249
           + NCN K+VGA  + +GYEA  G+++E   ++SPRD DGHGTHTA+T AG+ V  A+LF 
Sbjct: 192 KRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFG 251

Query: 250 QGMGVATGMRFTSRIAAYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKD 309
              G A GM   +R+AAYKVCW  GC ++DIL+A+D+AVADGV VLS+SLGGG S + +D
Sbjct: 252 FAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRD 311

Query: 310 EIAIATFGAVQKGVFVSCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNV 369
            ++IATFGA++ GVFVSCSAGN GP   ++ N +PWI TV AS  DR FPA VK+G    
Sbjct: 312 SLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRT 371

Query: 370 FEGSSLYYGNNTI----QLPLVY--NKTAGGNDANVCIPGSLVPSLVKGKIVICERGSNS 429
           F+G SLY G   +    Q PLVY     +  +  + C+ G+L    V GKIVIC+RG   
Sbjct: 372 FKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTP 431

Query: 430 RTAKGEVVKSAGGAGMILINTQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQP 489
           R  KG+VVK AGG GM+L NT   GEEL AD H+LPA  +G   GK I  Y  +S K   
Sbjct: 432 RVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKK--A 491

Query: 490 KGSITFEGTKYGSR-APRVAAFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELE 549
             S+   GT+ G + +P VAAFSSRGP+ +  +++KPD+ APGVNILAAW  +++P+ L 
Sbjct: 492 TASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLS 551

Query: 550 SDKRRVIFNIISGTSMSCPHASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISD 609
           SD RRV FNI+SGTSMSCPH SG+AAL+KS H  WSPAAIKSALMTTAY  DN  +P++D
Sbjct: 552 SDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTD 611

Query: 610 VGSESGKPADPFAFGSGHVDPEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISR-GN 669
             +    P+ P+  G+GH+DP +A+DPGL+YDI  Q+Y  +LC+   + +Q+ + ++  N
Sbjct: 612 --ASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSN 671

Query: 670 FTCPSKKQILQPGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIE 729
            TC        PG+LNYP+ S    +     ++T +RTVTNVG  +S Y V ++  KG  
Sbjct: 672 RTC-KHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGAS 731

Query: 730 ITVKPMKLNFGKLGEKLSYKVSFVSLGKREALGKFSFGSIVWISGKYAVRSPIAVTW 771
           +TV+P  LNF    +KLSY V+F +   R  + +  FG +VW S  + VRSP+ +TW
Sbjct: 732 VTVQPKTLNFTSKHQKLSYTVTFRT---RFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Sed0006414 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 3.9e-190
Identity = 366/752 (48.67%), Postives = 473/752 (62.90%), Query Frame = 0

Query: 28  EQTYII---HMDTTKMATTNPEQWYTSIIDSVNQLASLDDDEDEKSNVAEILYIYKTAIS 87
           ++TYII   H D  +   T+   WYTS ++S + L                LY Y T+  
Sbjct: 27  KKTYIIRVNHSDKPESFLTH-HDWYTSQLNSESSL----------------LYTYTTSFH 86

Query: 88  GFAAKLSNKKLNS-LSKIPGFLAATPNELLQLHTTHSPKFLGLQNSHGLWNSSNLASDII 147
           GF+A L + + +S LS     L    + L  LHTT +P+FLGL +  G+ +  + ++ +I
Sbjct: 87  GFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVI 146

Query: 148 IGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSNCNNKLVGASAYIKGYE-AI 207
           IGVLDTG+WPE  SF D  +P +P +WKG C++G  F    CN KL+GA ++ KG++ A 
Sbjct: 147 IGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMAS 206

Query: 208 VGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGMGVATGMRFTSRIAAYKVC 267
            G  +      SPRD DGHGTHT++TAAG+ V NAS      G A GM   +R+A YKVC
Sbjct: 207 GGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVC 266

Query: 268 WPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIAIATFGAVQKGVFVSCSAG 327
           W  GC  +DILAA+DRA+ DGVDVLSLSLGGG++ +Y+D IAI  F A+++GVFVSCSAG
Sbjct: 267 WSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAG 326

Query: 328 NSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEGSSLY--YGNNTIQLPLVY 387
           NSGP  ++V N APW+MTV A   DR FPA   LGNG    G SLY   G  T  L LVY
Sbjct: 327 NSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY 386

Query: 388 NKTAGGNDANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEVVKSAGGAGMILINTQLEG 447
           NK    + +N+C+PGSL  S+V+GKIV+C+RG N+R  KG VV+ AGG GMI+ NT   G
Sbjct: 387 NK-GNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASG 446

Query: 448 EELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFEGTKYGSR-APRVAAFSSR 507
           EEL AD H+LPA  +G   G  +  Y+ S SK  P   + F+GT    + +P VAAFSSR
Sbjct: 447 EELVADSHLLPAIAVGKKTGDLLREYVKSDSK--PTALLVFKGTVLDVKPSPVVAAFSSR 506

Query: 508 GPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIFNIISGTSMSCPHASGLA 567
           GP+ V  +++KPDV  PGVNILA W   + PT L+ D RR  FNI+SGTSMSCPH SGLA
Sbjct: 507 GPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLA 566

Query: 568 ALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGKPADPFAFGSGHVDPEKAS 627
            LLK+AH  WSP+AIKSALMTTAY  DN   P+ D    S   ++P+A GSGHVDP+KA 
Sbjct: 567 GLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNS--LSNPYAHGSGHVDPQKAL 626

Query: 628 DPGLIYDITNQDYLNYLCSLKYNATQI-ALISRGNFTCPSKKQILQPGDLNYPSFSVFMK 687
            PGL+YDI+ ++Y+ +LCSL Y    I A++ R +  C   K+   PG LNYPSFSV   
Sbjct: 627 SPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNC--SKKFSDPGQLNYPSFSVLFG 686

Query: 688 KKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLNFGKLGEKLSYKVSFVS 747
            K     V + R VTNVG   S Y V +N    + I+VKP KL+F  +GEK  Y V+FVS
Sbjct: 687 GKR---VVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVS 746

Query: 748 LGKREALGKFSFGSIVWISGKYAVRSPIAVTW 771
                   K  FGSI W + ++ VRSP+A +W
Sbjct: 747 KKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751

BLAST of Sed0006414 vs. ExPASy TrEMBL
Match: A0A6J1JPC3 (subtilisin-like protease SBT1.1 OS=Cucurbita maxima OX=3661 GN=LOC111487676 PE=3 SV=1)

HSP 1 Score: 1263.8 bits (3269), Expect = 0.0e+00
Identity = 636/767 (82.92%), Postives = 690/767 (89.96%), Query Frame = 0

Query: 5   MGFRGIWVLFSIMLVTSSNAALDEQTYIIHMDTTKMATTNPEQWYTSIIDSVNQLASLDD 64
           MGFR + +  SI L TS+ AA+D+Q+YIIHMDTTKMA  +PEQWYT++IDS+N+++SLDD
Sbjct: 1   MGFREVCLFLSIFLATSA-AAVDQQSYIIHMDTTKMAAPSPEQWYTALIDSINKISSLDD 60

Query: 65  DEDEKSNVAEILYIYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKFL 124
            E E S+ A+ILY+YKTAISGFAAKLS KKL+SLSK PGFLAATPNELLQLHTTHSP+FL
Sbjct: 61  QE-EASSAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFL 120

Query: 125 GLQNSHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSN 184
           GLQ  HGLWNSSNLASDI+IG+LDTGIWPEHISFQDKGLPPVPK+WKG CQAG KFS SN
Sbjct: 121 GLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSN 180

Query: 185 CNNKLVGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGM 244
           CN KL+GA+AYIKGYEAIVGRLN TGTFRSPRDSDGHGTHTASTAAGN+V  AS F+Q M
Sbjct: 181 CNRKLIGATAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAM 240

Query: 245 GVATGMRFTSRIAAYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIA 304
           GVATGMRFTSRIAAYKVCW EGCANADILAAIDRAVADGVDVLSLSLGG AS FYKD+IA
Sbjct: 241 GVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIA 300

Query: 305 IATFGAVQKGVFVSCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEG 364
           IATFGAV+ GVFVSCSAGNSGP SSTV N APWIMTVAASYTDRTFPA VKLGNG VFEG
Sbjct: 301 IATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFEG 360

Query: 365 SSLYYGNNTIQLPLVYNKTAGGNDANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEVVK 424
           SSLY GN+  QLPLVYN TAGG +ANVC  GSLVPSLVKGKIV+CERG+NSRTAKGE VK
Sbjct: 361 SSLYSGNSIGQLPLVYNNTAGGEEANVCTAGSLVPSLVKGKIVVCERGTNSRTAKGEQVK 420

Query: 425 SAGGAGMILINTQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFEGT 484
            AGGAGMILINTQLEGEELFADPHVLPA NLGASAG+AII YI SSSK+QPK  I FEGT
Sbjct: 421 LAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYI-SSSKHQPKALIAFEGT 480

Query: 485 KYGSRAPRVAAFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIFNI 544
           K+G+RAPRVAAFSSRGPS +  DVIKPDVTAPGVNILAAWPL  SP+ELESDKRRV+FNI
Sbjct: 481 KFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNI 540

Query: 545 ISGTSMSCPHASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGKPAD 604
           ISGTSMSCPH SGLAALLKSAHK WSPAAIKSALMTTAYT DNR  PISDVGSESGKPA+
Sbjct: 541 ISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSESGKPAN 600

Query: 605 PFAFGSGHVDPEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTCPSKKQILQ 664
           PFAFGSGHVDPEKASDPGLIYDIT QDYLNY CSL YN+TQI L+SRGNFTCPSK+++ Q
Sbjct: 601 PFAFGSGHVDPEKASDPGLIYDITPQDYLNYFCSLNYNSTQIGLVSRGNFTCPSKRRVGQ 660

Query: 665 PGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLNFG 724
           PG+LNYPSFSVFMKKKAKNVSVT KRTVTNVG P SDYTVKI NPKGI I V+P KL+F 
Sbjct: 661 PGNLNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYTVKIKNPKGIGIRVEPEKLSFR 720

Query: 725 KLGEKLSYKVSFVSLGKREALGKFSFGSIVWISGKYAVRSPIAVTWQ 772
           + G+KLSY+VSFV+LGKRE LG FSFGS+VW+SGKY VRSPIAVTW+
Sbjct: 721 RYGQKLSYQVSFVALGKREGLGGFSFGSLVWVSGKYGVRSPIAVTWK 764

BLAST of Sed0006414 vs. ExPASy TrEMBL
Match: A0A6J1FMR4 (subtilisin-like protease SBT1.1 OS=Cucurbita moschata OX=3662 GN=LOC111446877 PE=3 SV=1)

HSP 1 Score: 1254.6 bits (3245), Expect = 0.0e+00
Identity = 631/767 (82.27%), Postives = 686/767 (89.44%), Query Frame = 0

Query: 5   MGFRGIWVLFSIMLVTSSNAALDEQTYIIHMDTTKMATTNPEQWYTSIIDSVNQLASLDD 64
           MGFR + +  SI L TS+ AA+D+Q+YIIHMDTTKMA   PEQWYT++IDS+N+++SL+D
Sbjct: 1   MGFREVCLFLSIFLATST-AAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSLED 60

Query: 65  DEDEKSNVAEILYIYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKFL 124
            E E SN A+ILY+YKTAISGFAAKLS KKL+SLSK PGFLAATPNELLQLHTTHSP+FL
Sbjct: 61  QE-EASNAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFL 120

Query: 125 GLQNSHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSN 184
           GLQ  HGLWNSSNLASDI+IG+LDTGIWPEHISFQDKGLPPVPK+WKG CQAG KFS SN
Sbjct: 121 GLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSN 180

Query: 185 CNNKLVGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGM 244
           CN KL+GA AYIKGYEAIVGRLN TGTFRSPRDSDGHGTHTASTAAGN+V  AS F+Q M
Sbjct: 181 CNRKLIGAIAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAM 240

Query: 245 GVATGMRFTSRIAAYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIA 304
           GVATGMRFTSRIAAYKVCW EGCANADILAAIDRAVADGVDVLSLSLGG AS FYKD+IA
Sbjct: 241 GVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIA 300

Query: 305 IATFGAVQKGVFVSCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEG 364
           IATFGAV+ GVFVSCSAGNSGP SSTV N APWIMTVAASYTDR+FPA VKLGNG VFEG
Sbjct: 301 IATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRSFPATVKLGNGQVFEG 360

Query: 365 SSLYYGNNTIQLPLVYNKTAGGNDANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEVVK 424
           SSLY GNN  QLPLVYN TAGG DANVC  GSLVPS+VKGKIV+CERG+NSRTAKGE VK
Sbjct: 361 SSLYSGNNIGQLPLVYNNTAGGEDANVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQVK 420

Query: 425 SAGGAGMILINTQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFEGT 484
            AGGAGMILINTQLEGEELFADPHVLPA NLGASAG+AII YI SSSK+QPK  I FEGT
Sbjct: 421 LAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYI-SSSKHQPKALIAFEGT 480

Query: 485 KYGSRAPRVAAFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIFNI 544
           K+G+RAPRVAAFSSRGPS +  DVIKPDVTAPGVNILAAWPL  SP+E+ESDKRRV+FN+
Sbjct: 481 KFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSEVESDKRRVLFNV 540

Query: 545 ISGTSMSCPHASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGKPAD 604
           ISGTSMSCPH SGLAALLKSAHK WSPAAIKSALMTTAYT DNR  PISDVGS SGKPA+
Sbjct: 541 ISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPAN 600

Query: 605 PFAFGSGHVDPEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTCPSKKQILQ 664
           PFAFGSGHVDPEKASDPGLIYDIT QDYLNYLCSL YN+TQI L+SRGNFTCPSK+++ Q
Sbjct: 601 PFAFGSGHVDPEKASDPGLIYDITPQDYLNYLCSLNYNSTQIGLVSRGNFTCPSKRRVGQ 660

Query: 665 PGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLNFG 724
            G LNYPSFSVFMKKKAKNVSVT KRTVTNVG P SDY+VKI NPKGI I+VKP KL+F 
Sbjct: 661 AGKLNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYSVKIKNPKGIGISVKPEKLSFR 720

Query: 725 KLGEKLSYKVSFVSLGKREALGKFSFGSIVWISGKYAVRSPIAVTWQ 772
           + G+KLSY+VSFV+LGKRE +  FSFGS+VW+SG YAVRSPIAVTW+
Sbjct: 721 RYGQKLSYQVSFVALGKREGVSGFSFGSLVWVSGTYAVRSPIAVTWK 764

BLAST of Sed0006414 vs. ExPASy TrEMBL
Match: A0A1S3C0J8 (subtilisin-like protease SBT1.1 OS=Cucumis melo OX=3656 GN=LOC103495084 PE=3 SV=1)

HSP 1 Score: 1236.1 bits (3197), Expect = 0.0e+00
Identity = 627/768 (81.64%), Postives = 681/768 (88.67%), Query Frame = 0

Query: 5   MGFRGIWVLFSIMLVTSSNAALDEQTYIIHMDTTKMATTNPEQWYTSIIDSVNQLASLDD 64
           MGFR +WVL SIML  SS A +D+QTYIIHMDTTKM T NPEQWYT IIDSVN+L+SLDD
Sbjct: 1   MGFREVWVL-SIMLAISS-AVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSLDD 60

Query: 65  DEDEKSNVAEILYIYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKFL 124
           +E E SN AEILY+YKTA+SGFAAKL++KKL+SLSKIPGFLAATPNELLQLHTTHSP+FL
Sbjct: 61  NE-EASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFL 120

Query: 125 GLQNSHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSN 184
           GLQ  HGLWN SNLASDIIIG+LDTGIWPEHISFQDKGL  VP +WKGICQ GP+FS SN
Sbjct: 121 GLQRDHGLWNFSNLASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSSN 180

Query: 185 CNNKLVGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGM 244
           CN KL+GASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAG++V NAS ++QGM
Sbjct: 181 CNKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQGM 240

Query: 245 GVATGMRFTSRIAAYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIA 304
           GVA+GMRFTSRI AYKVCWP GCANADILAA+D AVADGVDVLSLSLGGG+S FYKD IA
Sbjct: 241 GVASGMRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIA 300

Query: 305 IATFGAVQKGVFVSCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEG 364
           IA FGA+QKGVFVSCSAGNSGP  STVGNAAPWIMTVAASYTDRTFP  VKLGNG VFEG
Sbjct: 301 IAAFGAIQKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEG 360

Query: 365 SSLYYGNNTIQLPLVYNKTAG-GNDANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEVV 424
           SSLYYG +  +LPLVYN TAG G + NVCI GSL PS+VKGKIVICERG+ SRT KGE V
Sbjct: 361 SSLYYGKSINELPLVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQV 420

Query: 425 KSAGGAGMILINTQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFEG 484
           K AGG GMILINTQ EGEELFADPHVLPAT LGASAGKAI++YI +SSK Q K SI FEG
Sbjct: 421 KLAGGTGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYI-ASSKTQAKASIVFEG 480

Query: 485 TKYGSRAPRVAAFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIFN 544
           TKYGS+APRVAAFSSRGPS VG DVIKPDVTAPGVNILAAWP  VSP+EL SD RRV+FN
Sbjct: 481 TKYGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELASDTRRVMFN 540

Query: 545 IISGTSMSCPHASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGKPA 604
           IISGTSMSCPH SGLAALLKSAH  WSPAAIKSALMTTAY TD++   ISDVG  +G+PA
Sbjct: 541 IISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPA 600

Query: 605 DPFAFGSGHVDPEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTCPSKKQIL 664
            PF FGSGHVDPEKASDPGLIYDIT QDY+NYLCSLKYN++QIAL+SRGN TC SK+ ++
Sbjct: 601 TPFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVV 660

Query: 665 QPGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLNF 724
           +PGDLNYPSFSVFMKKKAK VS+T KRTVTNVGI  SDYTVKINNPKG+ + VKP KL+F
Sbjct: 661 KPGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLSF 720

Query: 725 GKLGEKLSYKVSFVSLGKREALGKFSFGSIVWISGKYAVRSPIAVTWQ 772
           G LGE+LSYKVSFVSLG +EAL KFSFGS+VWISGKYAVRSPI VTWQ
Sbjct: 721 GSLGEQLSYKVSFVSLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 764

BLAST of Sed0006414 vs. ExPASy TrEMBL
Match: A0A5D3DZC9 (Subtilisin-like protease SBT1.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G001390 PE=3 SV=1)

HSP 1 Score: 1223.4 bits (3164), Expect = 0.0e+00
Identity = 615/755 (81.46%), Postives = 671/755 (88.87%), Query Frame = 0

Query: 18  LVTSSNAALDEQTYIIHMDTTKMATTNPEQWYTSIIDSVNQLASLDDDEDEKSNVAEILY 77
           ++  S+A +D+QTYIIHMDTTKM T NPEQWYT IIDSVN+L+SLDD+E E SN AEILY
Sbjct: 1   MLAISSAVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSLDDNE-EASNAAEILY 60

Query: 78  IYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKFLGLQNSHGLWNSSN 137
           +YKTA+SGFAAKL++KKL+SLSKIPGFLAATPNELLQLHTTHSP+FLGLQ  HGLWN SN
Sbjct: 61  VYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDHGLWNFSN 120

Query: 138 LASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSNCNNKLVGASAYIK 197
           LASDIIIG+LDTGIWPEHISFQDKGL  VP +WKGICQ GP+FS SNCN KL+GASAYIK
Sbjct: 121 LASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSSNCNKKLIGASAYIK 180

Query: 198 GYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGMGVATGMRFTSRIA 257
           GYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAG++V NAS ++QGMGVA+GMRFTSRI 
Sbjct: 181 GYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQGMGVASGMRFTSRIV 240

Query: 258 AYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIAIATFGAVQKGVFV 317
           AYKVCWP GCANADILAA+D AVADGVDVLSLSLGGG+S FYKD IAIA FGA+QKGVFV
Sbjct: 241 AYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIAAFGAIQKGVFV 300

Query: 318 SCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEGSSLYYGNNTIQLP 377
           SCSAGNSGP  STVGNAAPWIMTVAASYTDRTFP  VKLGNG VFEGSSLYYG +  +LP
Sbjct: 301 SCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYYGKSINELP 360

Query: 378 LVYNKTAG-GNDANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEVVKSAGGAGMILINT 437
           LVYN TAG G + NVCI GSL PS+VKGKIVICERG+ SRT KGE VK AGG GMILINT
Sbjct: 361 LVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQVKLAGGTGMILINT 420

Query: 438 QLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFEGTKYGSRAPRVAAF 497
           Q EGEELFADPHVLPAT LGASAGKAI++YI +SSK Q K SI FEGTKYGS+APRVAAF
Sbjct: 421 QFEGEELFADPHVLPATTLGASAGKAILDYI-ASSKTQAKASIVFEGTKYGSQAPRVAAF 480

Query: 498 SSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIFNIISGTSMSCPHAS 557
           SSRGPS VG DVIKPDVTAPGVNILAAWP  VSP+EL SD RRV+FNIISGTSMSCPH S
Sbjct: 481 SSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELASDTRRVMFNIISGTSMSCPHVS 540

Query: 558 GLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGKPADPFAFGSGHVDPE 617
           GLAALLKSAH  WSPAAIKSALMTTAY TD++   ISDVG  +G+PA PF FGSGHVDPE
Sbjct: 541 GLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPATPFTFGSGHVDPE 600

Query: 618 KASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTCPSKKQILQPGDLNYPSFSVF 677
           KASDPGLIYDIT QDY+NYLCSLKYN++QIAL+SRGN TC SK+ +++PGDLNYPSFSVF
Sbjct: 601 KASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVVKPGDLNYPSFSVF 660

Query: 678 MKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLNFGKLGEKLSYKVSF 737
           MKKKAK VS+T KRTVTNVGI  SDYTVKINNPKG+ + VKP KL+FG LGE+LSYKVSF
Sbjct: 661 MKKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLSFGSLGEQLSYKVSF 720

Query: 738 VSLGKREALGKFSFGSIVWISGKYAVRSPIAVTWQ 772
           VSLG +EAL KFSFGS+VWISGKYAVRSPI VTWQ
Sbjct: 721 VSLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 753

BLAST of Sed0006414 vs. ExPASy TrEMBL
Match: A0A6J1FT86 (subtilisin-like protease SBT1.1 OS=Cucurbita moschata OX=3662 GN=LOC111447921 PE=3 SV=1)

HSP 1 Score: 1221.5 bits (3159), Expect = 0.0e+00
Identity = 603/767 (78.62%), Postives = 674/767 (87.87%), Query Frame = 0

Query: 5   MGFRGIWVLFSIMLVTSSNAALDEQTYIIHMDTTKMATTNPEQWYTSIIDSVNQLASLDD 64
           MG   +W+L SIML TS+ AA D+Q+YIIHMD TKMATTNP+QWYTSIID++NQL+S++D
Sbjct: 1   MGITELWLLLSIMLATSAAAAADQQSYIIHMDATKMATTNPQQWYTSIIDTINQLSSIND 60

Query: 65  DEDEKSNVAEILYIYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKFL 124
           D++E SN AEILYIYKTAISGF+AKLS +KL+SLSK+PGFL+ATP++LLQLHTTH+PKFL
Sbjct: 61  DQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLSATPDKLLQLHTTHTPKFL 120

Query: 125 GLQNSHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSN 184
           GLQ  HGLWN+SNLASDIIIGV+DTGIWPEHISFQDKGLP VPK+WKG CQAGPKFSRSN
Sbjct: 121 GLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPAVPKKWKGTCQAGPKFSRSN 180

Query: 185 CNNKLVGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGM 244
           CN KL+GA+AYIKGYE I+GRLN TGTFRS RDSDGHGTHTASTAAGN+VY ASL++QGM
Sbjct: 181 CNKKLIGATAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGM 240

Query: 245 GVATGMRFTSRIAAYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIA 304
           G ATGMRFTSRIAAYKVCWPEGCA+ DILAAIDRAV DGVDVLSLSLGGG   FY+DEIA
Sbjct: 241 GAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIA 300

Query: 305 IATFGAVQKGVFVSCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEG 364
           IA FGAV+ GVFVSCSAGNSGPF STVGN APWIMTVAASYTDRTF   VKLGNG +FEG
Sbjct: 301 IAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFAGSVKLGNGQIFEG 360

Query: 365 SSLYYGNNTIQLPLVYNKTAGGNDANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEVVK 424
           SSL+ GN+  QLPLVYNKTAGG +ANVC  GSLVPS+VKGKIV+CERG+NSR  KGE VK
Sbjct: 361 SSLHSGNSIGQLPLVYNKTAGGEEANVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQVK 420

Query: 425 SAGGAGMILINTQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFEGT 484
            AGG GMILINTQLEGEELFAD HVLPA NLGASAGKAIINYI +SSK  PK SI FEGT
Sbjct: 421 LAGGVGMILINTQLEGEELFADSHVLPAVNLGASAGKAIINYI-ASSKQPPKASILFEGT 480

Query: 485 KYGSRAPRVAAFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIFNI 544
           +YGSRAPR+AAFSSRGPS     VIKPD+TAPGVNILAAWP  VSP+EL+SDKRRV+FNI
Sbjct: 481 RYGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNI 540

Query: 545 ISGTSMSCPHASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGKPAD 604
           ISGTSMSCPH SG+AALLKSAH +WSPAAIKSALMTTAY  DN+R  ISDVG  SG PAD
Sbjct: 541 ISGTSMSCPHVSGIAALLKSAHNNWSPAAIKSALMTTAYVNDNKRSVISDVGRPSGGPAD 600

Query: 605 PFAFGSGHVDPEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTCPSKKQILQ 664
           P+AFGSGHVDPEKA DPGL+YDI  QDYLNYLCSL Y + Q+ L+SRGNF+CPS + ++Q
Sbjct: 601 PYAFGSGHVDPEKAVDPGLVYDIEPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSNRNLVQ 660

Query: 665 PGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLNFG 724
           PGDLNYPSFSV MK +AKN  VTFKRTVTNVG P SDYTVKINNP GI ++VKP KL+F 
Sbjct: 661 PGDLNYPSFSVSMKNRAKN--VTFKRTVTNVGTPTSDYTVKINNPSGIRVSVKPKKLSFR 720

Query: 725 KLGEKLSYKVSFVSLGKREALGKFSFGSIVWISGKYAVRSPIAVTWQ 772
           + G+KL Y+VSFV+LGKRE L  FSFGS+VW+SGKY+VRSPIAV W+
Sbjct: 721 RSGQKLRYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Sed0006414 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 832.8 bits (2150), Expect = 2.3e-241
Identity = 429/769 (55.79%), Postives = 554/769 (72.04%), Query Frame = 0

Query: 5   MGFRGIWVLFSIMLVTSSNAALDEQTYIIHMDTTKMATTNPEQWYTSIIDSVNQLASLDD 64
           M F   +++F  ++  +SN +  +QTY+IH       TT+ +   TS+ +S+ Q  +++D
Sbjct: 18  MMFFRSFIVFFFLIFFASNVSSRKQTYVIH-----TVTTSTKHIVTSLFNSL-QTENIND 77

Query: 65  DEDEKSNVAEILYIYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKFL 124
           D+    ++ EI YIY+ A+SGF+A L++ +L+++    GF++A P+ELL LHTT+S +FL
Sbjct: 78  DD---FSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFL 137

Query: 125 GLQNSHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSN 184
           GL+   GLWN ++L+SD+IIG++DTGI PEH+SF+D  + PVP RW+G C  G  FS S 
Sbjct: 138 GLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSE 197

Query: 185 CNNKLVGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGM 244
           CN K++GASA+ KGYE+IVG++NET  FRS RD+ GHGTHTASTAAG++V  A+ F Q  
Sbjct: 198 CNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAK 257

Query: 245 GVATGMRFTSRIAAYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIA 304
           G+A+GMRFTSRIAAYK CW  GCA+ D++AAIDRA+ DGVDV+SLSLGG +  FY D IA
Sbjct: 258 GLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIA 317

Query: 305 IATFGAVQKGVFVSCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEG 364
           IA FGA+QK +FVSCSAGNSGP +STV N APW+MTVAASYTDRTFPAIV++GN     G
Sbjct: 318 IAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVG 377

Query: 365 SSLYYGNNTIQLPLVYNKTAGGNDANV-CIPGSLVPSLVKGKIVICERGSNSRTAKGEVV 424
           SSLY G +   LPL +N+TAG     V CI  SL   LV+GKIVIC RG++ RTAKGE V
Sbjct: 378 SSLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEV 437

Query: 425 KSAGGAGMILINTQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFEG 484
           K +GGA M+L++T+ EGEEL ADPHVLPA +LG S GK ++NY+  ++      S+ F G
Sbjct: 438 KRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAA--NATASVRFRG 497

Query: 485 TKYGSRAPRVAAFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIFN 544
           T YG+ AP VAAFSSRGPS  G ++ KPD+ APG+NILA W    SP+ L SD RRV FN
Sbjct: 498 TAYGATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFN 557

Query: 545 IISGTSMSCPHASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGK-P 604
           IISGTSM+CPH SG+AAL+KS H  WSPA IKSA+MTTA  TDNR RPI D G+   +  
Sbjct: 558 IISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESA 617

Query: 605 ADPFAFGSGHVDPEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTCPSKKQI 664
           A  FAFG+G+VDP +A DPGL+YD +  DYLNYLCSL Y + +I L S  N+TC S   +
Sbjct: 618 ATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVV 677

Query: 665 LQPGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLN 724
           L PGDLNYPSF+V +   A   +V +KRTVTNVG P  +Y V +  PKG+++ V+P  L 
Sbjct: 678 LSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLK 737

Query: 725 FGKLGEKLSYKVSFVSLGKREALGKFSFGSIVWISGKYAVRSPIAVTWQ 772
           F K  E+LSY V++ +   R +    SFG +VWI  KY VRSPIAVTW+
Sbjct: 738 FQKARERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Sed0006414 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 698.0 bits (1800), Expect = 8.7e-201
Identity = 374/758 (49.34%), Postives = 506/758 (66.75%), Query Frame = 0

Query: 22  SNAALDEQTYIIHMDTTKMATTNP--EQWYTSIIDSVNQLASLDDDEDEKSNVAEILYIY 81
           S+++ D+ TYI+HM  ++M ++      WY S + S+             S+ AE+LY Y
Sbjct: 23  SSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI-------------SDSAELLYTY 82

Query: 82  KTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKFLGL-QNSHGLWNSSNL 141
           + AI GF+ +L+ ++ +SL   PG ++  P    +LHTT +P FLGL +++  L+  +  
Sbjct: 83  ENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGS 142

Query: 142 ASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSNCNNKLVGASAYIKG 201
            SD+++GVLDTG+WPE  S+ D+G  P+P  WKG C+AG  F+ S CN KL+GA  + +G
Sbjct: 143 YSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARG 202

Query: 202 YEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGMGVATGMRFTSRIAA 261
           YE+ +G ++E+   RSPRD DGHGTHT+STAAG+VV  ASL     G A GM   +R+A 
Sbjct: 203 YESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAV 262

Query: 262 YKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIAIATFGAVQKGVFVS 321
           YKVCW  GC ++DILAAID+A+AD V+VLS+SLGGG S +Y+D +AI  F A+++G+ VS
Sbjct: 263 YKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVS 322

Query: 322 CSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEGSSLYYGNNTIQ--L 381
           CSAGN+GP SS++ N APWI TV A   DR FPA+  LGNG  F G SL+ G       L
Sbjct: 323 CSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLL 382

Query: 382 PLVY-NKTAGGNDANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEVVKSAGGAGMILIN 441
           P +Y    +   + N+C+ G+L+P  VKGKIV+C+RG N+R  KG+VVK+AGG GMIL N
Sbjct: 383 PFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILAN 442

Query: 442 TQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFEGTKYGSR-APRVA 501
           T   GEEL AD H+LPAT +G  AG  I +Y+T+     P  SI+  GT  G + +P VA
Sbjct: 443 TAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDP--NPTASISILGTVVGVKPSPVVA 502

Query: 502 AFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIFNIISGTSMSCPH 561
           AFSSRGP+ +  +++KPD+ APGVNILAAW     PT L SD RRV FNIISGTSMSCPH
Sbjct: 503 AFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPH 562

Query: 562 ASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGKPADPFAFGSGHVD 621
            SGLAALLKS H  WSPAAI+SALMTTAY T    +P+ D+   +GKP+ PF  G+GHV 
Sbjct: 563 VSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA--TGKPSTPFDHGAGHVS 622

Query: 622 PEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTC-PSKKQILQPGDLNYPSF 681
           P  A++PGLIYD+T +DYL +LC+L Y + QI  +SR N+TC PSK   +   DLNYPSF
Sbjct: 623 PTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSV--ADLNYPSF 682

Query: 682 SVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKI-NNPKGIEITVKPMKLNFGKLGEKLSY 741
           +V +       +  + RTVT+VG     Y+VK+ +   G++I+V+P  LNF +  EK SY
Sbjct: 683 AVNVDGVG---AYKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSY 742

Query: 742 KVSFVSLGKREALGKFSFGSIVWISGKYAVRSPIAVTW 771
            V+F ++   +  G  SFGSI W  GK+ V SP+A++W
Sbjct: 743 TVTF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Sed0006414 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 694.9 bits (1792), Expect = 7.4e-200
Identity = 385/777 (49.55%), Postives = 501/777 (64.48%), Query Frame = 0

Query: 13  LFSIMLVTSSNAALDEQTYIIHMDTTKMATTNPE--QWYTSIIDSVNQLASLDDDEDEKS 72
           L ++   +SS ++ +  TYI+H+D     +  P    WYTS       LASL       S
Sbjct: 10  LLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTS------SLASL------TS 69

Query: 73  NVAEILYIYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKFLGLQNSH 132
           +   I++ Y T   GF+A+L+++  + L   P  ++  P ++  LHTT SP+FLGL+++ 
Sbjct: 70  SPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTD 129

Query: 133 --GLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSNCNNK 192
             GL   S+  SD++IGV+DTG+WPE  SF D+GL PVP +WKG C A   F  S CN K
Sbjct: 130 KAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRK 189

Query: 193 LVGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGMGVAT 252
           LVGA  +  GYEA  G++NET  FRSPRDSDGHGTHTAS +AG  V+ AS      GVA 
Sbjct: 190 LVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAA 249

Query: 253 GMRFTSRIAAYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIAIATF 312
           GM   +R+AAYKVCW  GC ++DILAA D AVADGVDV+SLS+GG    +Y D IAI  F
Sbjct: 250 GMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAF 309

Query: 313 GAVQKGVFVSCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEGSSLY 372
           GA+ +G+FVS SAGN GP + TV N APW+ TV A   DR FPA VKLGNG +  G S+Y
Sbjct: 310 GAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVY 369

Query: 373 YG---NNTIQLPLVYNKTAGGND---ANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEV 432
            G   +     PLVY  +  G D   +++C+ GSL P+LVKGKIV+C+RG NSR  KGE+
Sbjct: 370 GGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEI 429

Query: 433 VKSAGGAGMILINTQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQ----PKGS 492
           V+  GG GMI+ N   +GE L AD HVLPAT++GAS G  I  YI+ SSK++    P  +
Sbjct: 430 VRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTAT 489

Query: 493 ITFEGTKYGSR-APRVAAFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDK 552
           I F+GT+ G R AP VA+FS+RGP+    +++KPDV APG+NILAAWP  + P+ + SD 
Sbjct: 490 IVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDN 549

Query: 553 RRVIFNIISGTSMSCPHASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGS 612
           RR  FNI+SGTSM+CPH SGLAALLK+AH  WSPAAI+SAL+TTAYT DN   P+ D   
Sbjct: 550 RRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD--E 609

Query: 613 ESGKPADPFAFGSGHVDPEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISRGNFTCP 672
            +G  +    +GSGHV P KA DPGL+YDIT+ DY+N+LC+  Y  T I  I+R    C 
Sbjct: 610 STGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCD 669

Query: 673 SKKQILQPGDLNYPSFS-VFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITV 732
             ++    G+LNYPSFS VF +     +S  F RTVTNVG   S Y +KI  P+G  +TV
Sbjct: 670 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTV 729

Query: 733 KPMKLNFGKLGEKLSYKVSFVSLGKREALG--KFSFGSIVWISGKYAVRSPIAVTWQ 772
           +P KL+F ++G+KLS+ V   +   + + G      G IVW  GK  V SP+ VT Q
Sbjct: 730 EPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of Sed0006414 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 682.2 bits (1759), Expect = 4.9e-196
Identity = 368/777 (47.36%), Postives = 500/777 (64.35%), Query Frame = 0

Query: 10  IWVLFSIMLV----TSSNAALDEQTYIIHMDTTKMAT--TNPEQWYTSIIDSVNQLASLD 69
           ++++ SI L+     ++     ++TY+IHMD + M    TN  QWY+S I+SV Q  S  
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS-- 71

Query: 70  DDEDEKSNVAEILYIYKTAISGFAAKLSNKKLNSLSKIPGFLAATPNELLQLHTTHSPKF 129
             ++E+ N   ILY Y+TA  G AA+L+ ++   L +  G +A  P    +LHTT SP F
Sbjct: 72  --QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTF 131

Query: 130 LGL--QNSHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFS 189
           LGL  Q S  +W       D+++GVLDTGIWPE  SF D G+ PVP  W+G C+ G +F 
Sbjct: 132 LGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFL 191

Query: 190 RSNCNNKLVGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFS 249
           + NCN K+VGA  + +GYEA  G+++E   ++SPRD DGHGTHTA+T AG+ V  A+LF 
Sbjct: 192 KRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFG 251

Query: 250 QGMGVATGMRFTSRIAAYKVCWPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKD 309
              G A GM   +R+AAYKVCW  GC ++DIL+A+D+AVADGV VLS+SLGGG S + +D
Sbjct: 252 FAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRD 311

Query: 310 EIAIATFGAVQKGVFVSCSAGNSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNV 369
            ++IATFGA++ GVFVSCSAGN GP   ++ N +PWI TV AS  DR FPA VK+G    
Sbjct: 312 SLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRT 371

Query: 370 FEGSSLYYGNNTI----QLPLVY--NKTAGGNDANVCIPGSLVPSLVKGKIVICERGSNS 429
           F+G SLY G   +    Q PLVY     +  +  + C+ G+L    V GKIVIC+RG   
Sbjct: 372 FKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTP 431

Query: 430 RTAKGEVVKSAGGAGMILINTQLEGEELFADPHVLPATNLGASAGKAIINYITSSSKNQP 489
           R  KG+VVK AGG GM+L NT   GEEL AD H+LPA  +G   GK I  Y  +S K   
Sbjct: 432 RVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKK--A 491

Query: 490 KGSITFEGTKYGSR-APRVAAFSSRGPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELE 549
             S+   GT+ G + +P VAAFSSRGP+ +  +++KPD+ APGVNILAAW  +++P+ L 
Sbjct: 492 TASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLS 551

Query: 550 SDKRRVIFNIISGTSMSCPHASGLAALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISD 609
           SD RRV FNI+SGTSMSCPH SG+AAL+KS H  WSPAAIKSALMTTAY  DN  +P++D
Sbjct: 552 SDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTD 611

Query: 610 VGSESGKPADPFAFGSGHVDPEKASDPGLIYDITNQDYLNYLCSLKYNATQIALISR-GN 669
             +    P+ P+  G+GH+DP +A+DPGL+YDI  Q+Y  +LC+   + +Q+ + ++  N
Sbjct: 612 --ASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSN 671

Query: 670 FTCPSKKQILQPGDLNYPSFSVFMKKKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIE 729
            TC        PG+LNYP+ S    +     ++T +RTVTNVG  +S Y V ++  KG  
Sbjct: 672 RTC-KHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGAS 731

Query: 730 ITVKPMKLNFGKLGEKLSYKVSFVSLGKREALGKFSFGSIVWISGKYAVRSPIAVTW 771
           +TV+P  LNF    +KLSY V+F +   R  + +  FG +VW S  + VRSP+ +TW
Sbjct: 732 VTVQPKTLNFTSKHQKLSYTVTFRT---RFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Sed0006414 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 666.4 bits (1718), Expect = 2.8e-191
Identity = 366/752 (48.67%), Postives = 473/752 (62.90%), Query Frame = 0

Query: 28  EQTYII---HMDTTKMATTNPEQWYTSIIDSVNQLASLDDDEDEKSNVAEILYIYKTAIS 87
           ++TYII   H D  +   T+   WYTS ++S + L                LY Y T+  
Sbjct: 27  KKTYIIRVNHSDKPESFLTH-HDWYTSQLNSESSL----------------LYTYTTSFH 86

Query: 88  GFAAKLSNKKLNS-LSKIPGFLAATPNELLQLHTTHSPKFLGLQNSHGLWNSSNLASDII 147
           GF+A L + + +S LS     L    + L  LHTT +P+FLGL +  G+ +  + ++ +I
Sbjct: 87  GFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVI 146

Query: 148 IGVLDTGIWPEHISFQDKGLPPVPKRWKGICQAGPKFSRSNCNNKLVGASAYIKGYE-AI 207
           IGVLDTG+WPE  SF D  +P +P +WKG C++G  F    CN KL+GA ++ KG++ A 
Sbjct: 147 IGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMAS 206

Query: 208 VGRLNETGTFRSPRDSDGHGTHTASTAAGNVVYNASLFSQGMGVATGMRFTSRIAAYKVC 267
            G  +      SPRD DGHGTHT++TAAG+ V NAS      G A GM   +R+A YKVC
Sbjct: 207 GGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVC 266

Query: 268 WPEGCANADILAAIDRAVADGVDVLSLSLGGGASIFYKDEIAIATFGAVQKGVFVSCSAG 327
           W  GC  +DILAA+DRA+ DGVDVLSLSLGGG++ +Y+D IAI  F A+++GVFVSCSAG
Sbjct: 267 WSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAG 326

Query: 328 NSGPFSSTVGNAAPWIMTVAASYTDRTFPAIVKLGNGNVFEGSSLY--YGNNTIQLPLVY 387
           NSGP  ++V N APW+MTV A   DR FPA   LGNG    G SLY   G  T  L LVY
Sbjct: 327 NSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY 386

Query: 388 NKTAGGNDANVCIPGSLVPSLVKGKIVICERGSNSRTAKGEVVKSAGGAGMILINTQLEG 447
           NK    + +N+C+PGSL  S+V+GKIV+C+RG N+R  KG VV+ AGG GMI+ NT   G
Sbjct: 387 NK-GNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASG 446

Query: 448 EELFADPHVLPATNLGASAGKAIINYITSSSKNQPKGSITFEGTKYGSR-APRVAAFSSR 507
           EEL AD H+LPA  +G   G  +  Y+ S SK  P   + F+GT    + +P VAAFSSR
Sbjct: 447 EELVADSHLLPAIAVGKKTGDLLREYVKSDSK--PTALLVFKGTVLDVKPSPVVAAFSSR 506

Query: 508 GPSGVGSDVIKPDVTAPGVNILAAWPLNVSPTELESDKRRVIFNIISGTSMSCPHASGLA 567
           GP+ V  +++KPDV  PGVNILA W   + PT L+ D RR  FNI+SGTSMSCPH SGLA
Sbjct: 507 GPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLA 566

Query: 568 ALLKSAHKSWSPAAIKSALMTTAYTTDNRRRPISDVGSESGKPADPFAFGSGHVDPEKAS 627
            LLK+AH  WSP+AIKSALMTTAY  DN   P+ D    S   ++P+A GSGHVDP+KA 
Sbjct: 567 GLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNS--LSNPYAHGSGHVDPQKAL 626

Query: 628 DPGLIYDITNQDYLNYLCSLKYNATQI-ALISRGNFTCPSKKQILQPGDLNYPSFSVFMK 687
            PGL+YDI+ ++Y+ +LCSL Y    I A++ R +  C   K+   PG LNYPSFSV   
Sbjct: 627 SPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNC--SKKFSDPGQLNYPSFSVLFG 686

Query: 688 KKAKNVSVTFKRTVTNVGIPMSDYTVKINNPKGIEITVKPMKLNFGKLGEKLSYKVSFVS 747
            K     V + R VTNVG   S Y V +N    + I+VKP KL+F  +GEK  Y V+FVS
Sbjct: 687 GKR---VVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVS 746

Query: 748 LGKREALGKFSFGSIVWISGKYAVRSPIAVTW 771
                   K  FGSI W + ++ VRSP+A +W
Sbjct: 747 KKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751