Sed0004659 (gene) Chayote v1

Overview


Name	Sed0004659
Type	gene
Organism	Sechium edule (Chayote v1)
Description	Valyl-tRNA synthetase
Location	LG05: 3836884 .. 3839163 (+)
RNA-Seq Expression	Sed0004659
Synteny	Sed0004659

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCACATTTTAAAGCTCGGGAAATGGTAGTCGACGCCTTGCAGAAGAAGGTACCATATTGTTCTTAGCTTGTTTTTCCTCAAACTGCTGCTCAATCATGGGGATGTGCTTTAGAAGGATTTGTTCTTCCAAAATGTTAATTACTAATGAATTTTGCCTTCTGGAAAATTGGGATAAAACTATCCTTTGTAACTTTCCTTTCCGTTGCACATTTTCTTTAAACGCTCATCCAAAATCCGTTAATGCAACTGAATTTTGTTTGCATTTAATGGTCTCTGTTTGGGATTGTTGATTTGGAATTTTAAATATTTTTCACCCCTTCATGCTGGCTTGTAAAAACTTGAAAATGTTTTGCAACTTTTTTTGTTGAAGCTAATCGCTCCACTAACAAGTTCGTTTTAATTTTGAATTACTTAATGCGTGAGAGTTGTTGGTTCATTTGAAGTTGCAAAATTATACTAAGTAATCTTTGAAGGTTGATGTATAATATTATTTATGGTCAACTAGATCTATAAGTCTGATGTACAAATTATTGTTTCTTATCTTCTATGGTTGTAGGCTCTTTATAGAGGTGCTAAATACAATGAGATGCGTCTTGTCCTAGAGTGCCTTTCCCTGAATAGAAGGGTAAATTTAGTATCCTAGCAAAACAGACACAAAAACTAGGAGTATTATTAATTTTACATTTGTACTGAATATGGTAATCACTTCTTTAAGTCATGATGCTCTTATCAATCGGGTTATATGGGAATCACAAAGTTGGGTGATTTATGAAAGCAAAGAAAACAAGTTTAAAAAGTCATTGGCAGCATATAATGAAACTCAACTATATTTTTGAAAGATTCTCTTCAATTATATTTGGAAAAGGCCCCTTTGGGACATCTGATAAAACTAACTTAGGAGATGGCAAGATTGCTTTTTTCTGGAATTATACCAAGTAATCTTTGAAGGTTGATGTATAATACATTTATGGTCAACTAGATTTATAAGGCTGATGTACAAATTATTGTTTCTTATCTTCTATGGTTGTAGGGTCTTGATAGAGGTGCTAAAGACAATGAGATGCATCTTGTCCTGGAGTGCCTTTCCCTGAATAGAAGGGTAAATTTAGTATCCTAGCAAAACAAACACAAAAACTAGGAGTATTATTAATTTTACATTTGTACTGAGTATGGTAATCACTTCTTTAAGTCATGATGCTCTTATCAATCGGGTTATATGGGAATCACAAAGTTGGGTGGTTTATGAAAGCAAAGAAAACAAGTTTAAAAAGTCATTGGCAGCATATAATGAAACTCAACTATATTTTTGAAAGATTCTCTTCAATTATGTTTGGAAAAGGCCCCTTTGGGACATTTGATAAAACTAGCTTAGGAGATGGCAAGATTGCTTTTTTCTGGAATGACACATTTGGGTTAGAAACCAATCAGTTTAGACTTCCTATTTTTGACCTCGTACTCCGAAAGATTTGTTTTTGGTTTATGAAATTCGGGAGGAGTTCTCTGGATTATGGAGATTTATGATTAGGAGAAATCTTATTGATGAGGATTGGTAGTTCTGCGGGGGGTTGGGTATTACAGTTGATTAAAGATGTTTTAATCACTAAAAGGAAAAGTAAGAGAAGCTGGAACTTAGATACTGCAGGGATTATTTTTCAGTAAAACCACTTGTTATTTTCTTCCTCTCCTAATGAAAGAGGGTAGCTGAAGTGGTTTCTTGAAAGTCACGGTGAAATCCTTGCTCCTAAATTAACCCTGCATTGGCTCCTCTTGAGGTGCCAGGTTTGAGGTGGATGTGGTGATTAATTTCTCAAATGACCCCAATTTCACTTTCAGTCCTAAGATGTATTCCGGTGCCAAGAAGATGAAAGTTTTCACGTGGATTTTCTCCCTCCAAGTTCCCTGGTCATCAGGAAAAAAAAAAAAAAAAACTTGTATTGAGTTGCGTTTCTGTGTCTTTAGATGAAAGTTTTCACTTGAATTTTTACGTCCAAGGCTGAGCATGTGCCTAGATTTAAAGAATAGGCATTAACCTCGACTCTCATTCCACCCTTATCTTTTTTCTCTGGCACGCTAAACATTATTTTAATATAGATTGTTTGCTACCGGTGGCTAGAGAACATTTGTGATTGGTGCATCTCAAGGCAACTTTGGTGGGGTCACAGAATTCCTGCAAGGTATGCTACATTAGAGGGTGATCAACTAAAGGAGTTTGGTGCATATAATGATCACTGGGTGGTTGCAAGAAATGAGGAAGAGGCTGAGAAGGAGGCCAAATAA

mRNA sequence

ATGCCACATTTTAAAGCTCGGGAAATGGTAGTCGACGCCTTGCAGAAGAAGGCTCTTTATAGAGGTGCTAAATACAATGAGATGCGTCTTGTCCTAGAGTGCCTTTCCCTGAATAGAAGGGGTCTTGATAGAGGTGCTAAAGACAATGAGATGCATCTTGTCCTGGAGTGCCTTTCCCTGAATAGAAGGATTGTTTGCTACCGGTGGCTAGAGAACATTTGTGATTGGTGCATCTCAAGGCAACTTTGGTGGGGTCACAGAATTCCTGCAAGGTATGCTACATTAGAGGGTGATCAACTAAAGGAGTTTGGTGCATATAATGATCACTGGGTGGTTGCAAGAAATGAGGAAGAGGCTGAGAAGGAGGCCAAATAA

Coding sequence (CDS)

ATGCCACATTTTAAAGCTCGGGAAATGGTAGTCGACGCCTTGCAGAAGAAGGCTCTTTATAGAGGTGCTAAATACAATGAGATGCGTCTTGTCCTAGAGTGCCTTTCCCTGAATAGAAGGGGTCTTGATAGAGGTGCTAAAGACAATGAGATGCATCTTGTCCTGGAGTGCCTTTCCCTGAATAGAAGGATTGTTTGCTACCGGTGGCTAGAGAACATTTGTGATTGGTGCATCTCAAGGCAACTTTGGTGGGGTCACAGAATTCCTGCAAGGTATGCTACATTAGAGGGTGATCAACTAAAGGAGTTTGGTGCATATAATGATCACTGGGTGGTTGCAAGAAATGAGGAAGAGGCTGAGAAGGAGGCCAAATAA

Protein sequence

MPHFKAREMVVDALQKKALYRGAKYNEMRLVLECLSLNRRGLDRGAKDNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKEFGAYNDHWVVARNEEEAEKEAK

Homology

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BLAST of Sed0004659 vs. NCBI nr
Match: TYK00287.1 (valine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 157.9 bits (398), Expect = 5.6e-35
Identity = 87/142 (61.27%), Postives = 94/142 (66.20%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEMRLVL------------------ECLSLNRRGL 60
           MP FKAREMVVDALQKK LYRGAK NEMRL L                   C    ++ L
Sbjct: 398 MPRFKAREMVVDALQKKGLYRGAKDNEMRLGLCSRTNDVVEPMIKPQWYVNCKEPAKQSL 457

Query: 61  DRGAKDNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKE 120
           D  A DNE   + + +         RWL+NI DWCISRQLWWGHRIPA YATLE DQLKE
Sbjct: 458 D-AAMDNECKKI-DIIPKQYSADWKRWLDNIRDWCISRQLWWGHRIPAWYATLEDDQLKE 517

Query: 121 FGAYNDHWVVARNEEEAEKEAK 125
           FGAYNDHWVVARNEEEAE+EAK
Sbjct: 518 FGAYNDHWVVARNEEEAEEEAK 537

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BLAST of Sed0004659 vs. NCBI nr
Match: KAG6605705.1 (Valine--tRNA ligase, mitochondrial 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 157.9 bits (398), Expect = 5.6e-35
Identity = 86/142 (60.56%), Postives = 93/142 (65.49%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEMRLVL------------------ECLSLNRRGL 60
           MP FK REMVVDALQKK LYRGAK NEMRL L                   C  + ++ L
Sbjct: 456 MPRFKVREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSL 515

Query: 61  DRGAKDNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKE 120
           D    DNE   + E +         RWLENI DWCISRQLWWGHRIPA YATL+ DQLKE
Sbjct: 516 D-AVMDNECKKI-EIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKE 575

Query: 121 FGAYNDHWVVARNEEEAEKEAK 125
           FGAYNDHWVVARNEEEAE+EAK
Sbjct: 576 FGAYNDHWVVARNEEEAEEEAK 595

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BLAST of Sed0004659 vs. NCBI nr
Match: KAG7035615.1 (Valine--tRNA ligase, mitochondrial 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 157.9 bits (398), Expect = 5.6e-35
Identity = 86/142 (60.56%), Postives = 93/142 (65.49%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEMRLVL------------------ECLSLNRRGL 60
           MP FK REMVVDALQKK LYRGAK NEMRL L                   C  + ++ L
Sbjct: 474 MPRFKVREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSL 533

Query: 61  DRGAKDNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKE 120
           D    DNE   + E +         RWLENI DWCISRQLWWGHRIPA YATL+ DQLKE
Sbjct: 534 D-AVMDNECKKI-EIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKE 593

Query: 121 FGAYNDHWVVARNEEEAEKEAK 125
           FGAYNDHWVVARNEEEAE+EAK
Sbjct: 594 FGAYNDHWVVARNEEEAEEEAK 613

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BLAST of Sed0004659 vs. NCBI nr
Match: XP_023533939.1 (valine--tRNA ligase, mitochondrial 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 157.9 bits (398), Expect = 5.6e-35
Identity = 86/142 (60.56%), Postives = 93/142 (65.49%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEMRLVL------------------ECLSLNRRGL 60
           MP FK REMVVDALQKK LYRGAK NEMRL L                   C  + ++ L
Sbjct: 474 MPRFKVREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSL 533

Query: 61  DRGAKDNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKE 120
           D    DNE   + E +         RWLENI DWCISRQLWWGHRIPA YATL+ DQLKE
Sbjct: 534 D-AVTDNECKKI-EIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKE 593

Query: 121 FGAYNDHWVVARNEEEAEKEAK 125
           FGAYNDHWVVARNEEEAE+EAK
Sbjct: 594 FGAYNDHWVVARNEEEAEEEAK 613

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BLAST of Sed0004659 vs. NCBI nr
Match: XP_008467213.1 (PREDICTED: valine--tRNA ligase, mitochondrial 1 [Cucumis melo])

HSP 1 Score: 157.9 bits (398), Expect = 5.6e-35
Identity = 87/142 (61.27%), Postives = 94/142 (66.20%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEMRLVL------------------ECLSLNRRGL 60
           MP FKAREMVVDALQKK LYRGAK NEMRL L                   C    ++ L
Sbjct: 413 MPRFKAREMVVDALQKKGLYRGAKDNEMRLGLCSRTNDVVEPMIKPQWYVNCKEPAKQSL 472

Query: 61  DRGAKDNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKE 120
           D  A DNE   + + +         RWL+NI DWCISRQLWWGHRIPA YATLE DQLKE
Sbjct: 473 D-AAMDNECKKI-DIIPKQYSADWKRWLDNIRDWCISRQLWWGHRIPAWYATLEDDQLKE 532

Query: 121 FGAYNDHWVVARNEEEAEKEAK 125
           FGAYNDHWVVARNEEEAE+EAK
Sbjct: 533 FGAYNDHWVVARNEEEAEEEAK 552

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BLAST of Sed0004659 vs. ExPASy Swiss-Prot
Match: P93736 (Valine--tRNA ligase, mitochondrial 1 OS=Arabidopsis thaliana OX=3702 GN=TWN2 PE=1 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 3.5e-32
Identity = 76/141 (53.90%), Postives = 85/141 (60.28%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEMRLVL------------------ECLSLNRRGL 60
           MP F ARE VV+ALQK+ LYRGAK NEMRL L                   C  + +  L
Sbjct: 464 MPRFAAREAVVEALQKQGLYRGAKNNEMRLGLCSRTNDVIEPMIKPQWYVNCSMIGKEAL 523

Query: 61  DRGAKDNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKE 120
           D    D      LE +         RWLENI DWCISRQLWWGHRIPA YATLE DQLKE
Sbjct: 524 DVAITDENKK--LEFVPKQYTAEWRRWLENIRDWCISRQLWWGHRIPAWYATLEEDQLKE 583

Query: 121 FGAYNDHWVVARNEEEAEKEA 124
            GAY+DHWVVAR E++A +EA
Sbjct: 584 VGAYSDHWVVARTEDDAREEA 602

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BLAST of Sed0004659 vs. ExPASy Swiss-Prot
Match: Q86KU2 (Probable valine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=valS1 PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 4.2e-17
Identity = 50/141 (35.46%), Postives = 75/141 (53.19%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEMRLVL------------------ECLSLNRRGL 60
           M  F AR  VV+AL++K L++G K N+MRL L                  +C  +  R +
Sbjct: 438 MKRFDARNAVVEALKEKNLFKGMKDNKMRLGLCSRSKDVIEPMIKPQWYVKCDDMAARAV 497

Query: 61  DRGAKDNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKE 120
            +  +D ++ +  +       +  YRWLE I DWC+SRQLWWGHRIPA +  ++G +   
Sbjct: 498 -KAVRDGDLKITPK----THEVTWYRWLEGIKDWCVSRQLWWGHRIPAYHCIIKGVKSNP 557

Query: 121 FGAYNDHWVVARNEEEAEKEA 124
           +    + WVV  N+EEA + A
Sbjct: 558 YD--TNQWVVGANQEEATQNA 571

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BLAST of Sed0004659 vs. ExPASy Swiss-Prot
Match: P07806 (Valine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=VAS1 PE=1 SV=2)

HSP 1 Score: 78.2 bits (191), Expect = 7.4e-14
Identity = 46/136 (33.82%), Postives = 69/136 (50.74%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEMRL--------VLECLSLNRRGLDRGAKDNEMH 60
           M  F AR+ V++ L++K LY G + NEM +        ++E L   +  + +     +  
Sbjct: 477 MKRFDARKKVIEQLKEKNLYVGQEDNEMTIPTCSRSGDIIEPLLKPQWWVSQSEMAKDAI 536

Query: 61  LV-----LECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKEFGAYN 120
            V     +     +     + WL NI DWCISRQLWWGHR P  +  +EG++        
Sbjct: 537 KVVRDGQITITPKSSEAEYFHWLGNIQDWCISRQLWWGHRCPVYFINIEGEEHDRID--G 596

Query: 121 DHWVVARNEEEAEKEA 124
           D+WV  R+ EEAEK+A
Sbjct: 597 DYWVAGRSMEEAEKKA 610

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BLAST of Sed0004659 vs. ExPASy Swiss-Prot
Match: O75005 (Valine--tRNA ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=vas2 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 2.8e-13
Identity = 48/137 (35.04%), Postives = 71/137 (51.82%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEMRL--------VLECLS-----LNRRGLDRGAK 60
           M  F AR  VV+ L++  L+ G K N M +        ++E +      +N++ +   A 
Sbjct: 425 MKRFTARVKVVERLKELGLFVGTKENPMVIPLCGKTSDIIEPVMKPQWWVNQKEMAAAAA 484

Query: 61  DNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKEFGAYN 120
           +      +E      R    RW+ENI DWCISRQLWWGHRIPA +  L  D+  +  +  
Sbjct: 485 EVVKSGEIEIAPDMSRREFIRWMENIQDWCISRQLWWGHRIPAYFVNL-ADEPSQDRSEG 544

Query: 121 DHWVVARNEEEAEKEAK 125
            +WV  R  E+AE++AK
Sbjct: 545 RYWVTGRTLEQAEEKAK 560

[Close]

BLAST of Sed0004659 vs. ExPASy Swiss-Prot
Match: Q9U1Q4 (Valine--tRNA ligase OS=Caenorhabditis elegans OX=6239 GN=glp-4 PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 4.8e-13
Identity = 47/141 (33.33%), Postives = 69/141 (48.94%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEM------------------RLVLECLSLNRRGL 60
           M  F AR  V++AL++K LYRG + N M                  +  ++C  +  + +
Sbjct: 413 MKRFDARTAVIEALKEKGLYRGKEDNPMVVPTCSRSKDVIEPILKPQWYVKCAHMAEKAV 472

Query: 61  DRGAKDNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKE 120
              A  +     L+ +    +    RWLE+  DWCISRQLWWGHRIPA Y +    + + 
Sbjct: 473 AAVANGD-----LQIIPEFHKATWNRWLESSRDWCISRQLWWGHRIPAYYISFADGREQP 532

Query: 121 FGAYNDHWVVARNEEEAEKEA 124
               N +WV AR E+EA  +A
Sbjct: 533 LPEEN-YWVSARTEQEALAKA 547

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BLAST of Sed0004659 vs. ExPASy TrEMBL
Match: A0A5D3BM83 (Valyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00290 PE=3 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.7e-35
Identity = 87/142 (61.27%), Postives = 94/142 (66.20%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEMRLVL------------------ECLSLNRRGL 60
           MP FKAREMVVDALQKK LYRGAK NEMRL L                   C    ++ L
Sbjct: 398 MPRFKAREMVVDALQKKGLYRGAKDNEMRLGLCSRTNDVVEPMIKPQWYVNCKEPAKQSL 457

Query: 61  DRGAKDNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKE 120
           D  A DNE   + + +         RWL+NI DWCISRQLWWGHRIPA YATLE DQLKE
Sbjct: 458 D-AAMDNECKKI-DIIPKQYSADWKRWLDNIRDWCISRQLWWGHRIPAWYATLEDDQLKE 517

Query: 121 FGAYNDHWVVARNEEEAEKEAK 125
           FGAYNDHWVVARNEEEAE+EAK
Sbjct: 518 FGAYNDHWVVARNEEEAEEEAK 537

[Close]

BLAST of Sed0004659 vs. ExPASy TrEMBL
Match: A0A1S3CT04 (Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103504618 PE=3 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.7e-35
Identity = 87/142 (61.27%), Postives = 94/142 (66.20%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEMRLVL------------------ECLSLNRRGL 60
           MP FKAREMVVDALQKK LYRGAK NEMRL L                   C    ++ L
Sbjct: 413 MPRFKAREMVVDALQKKGLYRGAKDNEMRLGLCSRTNDVVEPMIKPQWYVNCKEPAKQSL 472

Query: 61  DRGAKDNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKE 120
           D  A DNE   + + +         RWL+NI DWCISRQLWWGHRIPA YATLE DQLKE
Sbjct: 473 D-AAMDNECKKI-DIIPKQYSADWKRWLDNIRDWCISRQLWWGHRIPAWYATLEDDQLKE 532

Query: 121 FGAYNDHWVVARNEEEAEKEAK 125
           FGAYNDHWVVARNEEEAE+EAK
Sbjct: 533 FGAYNDHWVVARNEEEAEEEAK 552

[Close]

BLAST of Sed0004659 vs. ExPASy TrEMBL
Match: A0A6J1JYM1 (Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111490926 PE=3 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 7.9e-35
Identity = 85/142 (59.86%), Postives = 93/142 (65.49%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEMRLVL------------------ECLSLNRRGL 60
           MP FK REMVVDALQKK LYRGAK NEMRL L                   C  + ++ L
Sbjct: 475 MPRFKVREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSL 534

Query: 61  DRGAKDNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKE 120
           D    DNE   + + +         RWLENI DWCISRQLWWGHRIPA YATL+ DQLKE
Sbjct: 535 D-AVTDNECKKI-KIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKE 594

Query: 121 FGAYNDHWVVARNEEEAEKEAK 125
           FGAYNDHWVVARNEEEAE+EAK
Sbjct: 595 FGAYNDHWVVARNEEEAEEEAK 614

[Close]

BLAST of Sed0004659 vs. ExPASy TrEMBL
Match: A0A6J1H3K8 (Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111459332 PE=3 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 7.9e-35
Identity = 85/142 (59.86%), Postives = 93/142 (65.49%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEMRLVL------------------ECLSLNRRGL 60
           MP F+ REMVVDALQKK LYRGAK NEMRL L                   C  + ++ L
Sbjct: 474 MPRFEVREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSL 533

Query: 61  DRGAKDNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKE 120
           D    DNE   + E +         RWLENI DWCISRQLWWGHRIPA YATL+ DQLKE
Sbjct: 534 D-AVTDNECKKI-EIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKE 593

Query: 121 FGAYNDHWVVARNEEEAEKEAK 125
           FGAYNDHWVVARNEEEAE+EAK
Sbjct: 594 FGAYNDHWVVARNEEEAEEEAK 613

[Close]

BLAST of Sed0004659 vs. ExPASy TrEMBL
Match: A0A6J1H1R1 (Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111459332 PE=3 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 7.9e-35
Identity = 85/142 (59.86%), Postives = 93/142 (65.49%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEMRLVL------------------ECLSLNRRGL 60
           MP F+ REMVVDALQKK LYRGAK NEMRL L                   C  + ++ L
Sbjct: 333 MPRFEVREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSL 392

Query: 61  DRGAKDNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKE 120
           D    DNE   + E +         RWLENI DWCISRQLWWGHRIPA YATL+ DQLKE
Sbjct: 393 D-AVTDNECKKI-EIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKE 452

Query: 121 FGAYNDHWVVARNEEEAEKEAK 125
           FGAYNDHWVVARNEEEAE+EAK
Sbjct: 453 FGAYNDHWVVARNEEEAEEEAK 472

[Close]

BLAST of Sed0004659 vs. TAIR 10
Match: AT1G14610.1 (valyl-tRNA synthetase / valine--tRNA ligase (VALRS) )

HSP 1 Score: 139.0 bits (349), Expect = 2.5e-33
Identity = 76/141 (53.90%), Postives = 85/141 (60.28%), Query Frame = 0


Query: 1   MPHFKAREMVVDALQKKALYRGAKYNEMRLVL------------------ECLSLNRRGL 60
           MP F ARE VV+ALQK+ LYRGAK NEMRL L                   C  + +  L
Sbjct: 464 MPRFAAREAVVEALQKQGLYRGAKNNEMRLGLCSRTNDVIEPMIKPQWYVNCSMIGKEAL 523

Query: 61  DRGAKDNEMHLVLECLSLNRRIVCYRWLENICDWCISRQLWWGHRIPARYATLEGDQLKE 120
           D    D      LE +         RWLENI DWCISRQLWWGHRIPA YATLE DQLKE
Sbjct: 524 DVAITDENKK--LEFVPKQYTAEWRRWLENIRDWCISRQLWWGHRIPAWYATLEEDQLKE 583

Query: 121 FGAYNDHWVVARNEEEAEKEA 124
            GAY+DHWVVAR E++A +EA
Sbjct: 584 VGAYSDHWVVARTEDDAREEA 602

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BLAST of Sed0004659 vs. TAIR 10
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 55.8 bits (133), Expect = 2.8e-08
Identity = 28/55 (50.91%), Postives = 35/55 (63.64%), Query Frame = 0


Query: 69  WLENICDWCISRQLWWGHRIPARYATLEGDQLKEFGAYNDHWVVARNEEEAEKEA 124
           WL NI DWCISRQLWWGHRIP  Y    G   +E       ++VA++ EEA ++A
Sbjct: 459 WLTNIKDWCISRQLWWGHRIPVWYVV--GKDCEE------DYIVAKSAEEALEKA 505

The following BLAST results are available for this feature:

BLAST of Sed0004659 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK00287.1	5.6e-35	61.27	valine--tRNA ligase [Cucumis melo var. makuwa]	[more]
KAG6605705.1	5.6e-35	60.56	Valine--tRNA ligase, mitochondrial 1, partial [Cucurbita argyrosperma subsp. sor...	[more]
KAG7035615.1	5.6e-35	60.56	Valine--tRNA ligase, mitochondrial 1 [Cucurbita argyrosperma subsp. argyrosperma...	[more]
XP_023533939.1	5.6e-35	60.56	valine--tRNA ligase, mitochondrial 1 [Cucurbita pepo subsp. pepo]	[more]
XP_008467213.1	5.6e-35	61.27	PREDICTED: valine--tRNA ligase, mitochondrial 1 [Cucumis melo]	[more]

BLAST of Sed0004659 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P93736	3.5e-32	53.90	Valine--tRNA ligase, mitochondrial 1 OS=Arabidopsis thaliana OX=3702 GN=TWN2 PE=...	[more]
Q86KU2	4.2e-17	35.46	Probable valine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 G...	[more]
P07806	7.4e-14	33.82	Valine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 2045...	[more]
O75005	2.8e-13	35.04	Valine--tRNA ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28...	[more]
Q9U1Q4	4.8e-13	33.33	Valine--tRNA ligase OS=Caenorhabditis elegans OX=6239 GN=glp-4 PE=1 SV=1	[more]

BLAST of Sed0004659 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BM83	2.7e-35	61.27	Valyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold17...	[more]
A0A1S3CT04	2.7e-35	61.27	Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103504618 PE=3 SV=1	[more]
A0A6J1JYM1	7.9e-35	59.86	Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111490926 PE=3 SV=1	[more]
A0A6J1H3K8	7.9e-35	59.86	Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111459332 PE=3 SV=1	[more]
A0A6J1H1R1	7.9e-35	59.86	Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111459332 PE=3 SV=1	[more]

BLAST of Sed0004659 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14610.1	2.5e-33	53.90	valyl-tRNA synthetase / valine--tRNA ligase (VALRS)	[more]
AT5G16715.1	2.8e-08	50.91	ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP bi...	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 33..124 e-value: 1.4E-11 score: 45.9
IPR002300	Aminoacyl-tRNA synthetase, class Ia	PFAM	PF00133	tRNA-synt_1	coord: 66..122 e-value: 2.9E-12 score: 45.7
None	No IPR available	PANTHER	PTHR11946:SF113	BNAA08G31530D PROTEIN	coord: 1..123
None	No IPR available	SUPERFAMILY	52374	Nucleotidylyl transferase	coord: 61..93
IPR002303	Valine-tRNA ligase	PANTHER	PTHR11946	VALYL-TRNA SYNTHETASES	coord: 1..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0004659.1	Sed0004659.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006438	valyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004832	valine-tRNA ligase activity
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding