Sed0004524 (gene) Chayote v1

BLAST of Sed0004524 vs. NCBI nr
Match: XP_023550545.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1173.3 bits (3034), Expect = 0.0e+00
Identity = 596/739 (80.65%), Postives = 648/739 (87.69%), Query Frame = 0

Query: 1   MLSLPKLLFLSFCFSSF---------QARTYIVYMGSHSKDKVSTSSHHMRMLQETIGST 60
           M SL +LLFL+FCFS             +TYIVYMGSH KD+VST SHH+RMLQETIGS+
Sbjct: 1   MSSLSRLLFLAFCFSRLFFSSNSEHEDRKTYIVYMGSHPKDRVSTRSHHVRMLQETIGSS 60

Query: 61  FSPHRLLHSYKRSFNGFVVKLSEAEAQKISEMEGVISVFRNGRKELHTTRSWDFMGLSQQ 120
           F+PH LLHSY+RSFNGFV KL+E E QK+SEM+GVISVF NG+K+LHTTRSWDFMGLSQQ
Sbjct: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSIESDIIVGVFDSGIWPESPSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARS 180
           ASRVPS+ESDIIVGV D+GIWPESPSF D GY PPP KWKG CEVS++FSCNNKIIGARS
Sbjct: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPRKWKGSCEVSSDFSCNNKIIGARS 180

Query: 181 YRSDGLYSQTDLQSPRDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYK 240
           YR++G Y   D+Q PRDS+GHGTH ASTVAGGLVRQASMLGLG GTARGGVPSARIA+YK
Sbjct: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGGLVRQASMLGLGSGTARGGVPSARIASYK 240

Query: 241 ICWSDGCYDADILAAFDDAIADGVDIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSM 300
           ICWSDGC DAD+LAAFDDAIADGVDIISFSVGG  P+ YFNDSIAIGAFHAMKKGILTSM
Sbjct: 241 ICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSM 300

Query: 301 SAGNDGPDFFTIRNFSPWSLSVAASTTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPL 360
           SAGN GP  FT+RNFSPWSLSVAASTTDR+FL+GVQLGDG +F GVTINTFDLNGTQYPL
Sbjct: 301 SAGNSGPKSFTVRNFSPWSLSVAASTTDRKFLSGVQLGDGRSFDGVTINTFDLNGTQYPL 360

Query: 361 VYAGDIPNITAGFNASRSRFCLENSVDKELVRGKITLCDSILSPKELGSLKNAIGIIMQD 420
           VYAG+IPNI  GFN S SRFCL NSVDKELV+GKI LCD  +SP  L  L+ AIGIIMQD
Sbjct: 361 VYAGNIPNI--GFNGSISRFCLTNSVDKELVKGKIVLCDFFVSPTNLSFLEGAIGIIMQD 420

Query: 421 RSPKDLTSSFPLPASHLGTQQG-LISSYINLTSLPTATILKSTEG--KTSTFVASFSSRG 480
            +PKDLT  FPLPASHLGTQ+G LISSY NLTSLPTATILKSTEG  K + FVASFSSRG
Sbjct: 421 NNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKETPFVASFSSRG 480

Query: 481 PNPITLDILKPDLSGPGVEILAAWSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAA 540
           PNPIT DILKPDLSGPGVEILAAWSPIG PSGA+DD R +L+NIISGTSMSCPHATAVAA
Sbjct: 481 PNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEDDTRQLLFNIISGTSMSCPHATAVAA 540

Query: 541 YVKSFHPSWSPAALKSALMTTAFPMKAELNSDAEFAYGSGHINPLSAINPGLIYNATEID 600
           YVKSFHPSWSPAALKSALMTTAFPM+A+LN DAEFAYGSGHINPLSA+NPGLIYNATEID
Sbjct: 541 YVKSFHPSWSPAALKSALMTTAFPMRADLNPDAEFAYGSGHINPLSAVNPGLIYNATEID 600

Query: 601 YIRFLCGQGYSTKQLQQVSEDNNTCNSANNFDTVFNLNYPSFALSTTISTPISQVYKRRV 660
           Y+RFLCGQGYSTK +QQVS DN++C S  + D VF+LNYPSFALST+IST ISQVY+RRV
Sbjct: 601 YVRFLCGQGYSTKLVQQVSGDNSSC-SRGDIDLVFDLNYPSFALSTSISTSISQVYRRRV 660

Query: 661 TNVGSANSTYKASIFAPLGLNIIVNPSVLSFKALGEEKNFEVTIEGSIDSGIASASLVWD 720
           TNVGSANSTYKA +  PLGLNI VNPSVLSFKALGEE  FEVTIEGSI S IAS SLVWD
Sbjct: 661 TNVGSANSTYKAIVSGPLGLNITVNPSVLSFKALGEELRFEVTIEGSISSSIASGSLVWD 720

Query: 721 DGLHKVRSPITVFDANTFV 728
           DG HKV+SPI VFD NTF+
Sbjct: 721 DGKHKVKSPIVVFDENTFI 736

BLAST of Sed0004524 vs. NCBI nr
Match: XP_022922167.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 590/739 (79.84%), Postives = 646/739 (87.42%), Query Frame = 0

Query: 1   MLSLPKLLFLSFCFSSF---------QARTYIVYMGSHSKDKVSTSSHHMRMLQETIGST 60
           M SL +LLFL+F  S             RTYIVYMGSH KD+VST SHH+RMLQETIGS+
Sbjct: 1   MSSLSRLLFLAFWLSRLFFSSNSEDDDKRTYIVYMGSHPKDRVSTRSHHVRMLQETIGSS 60

Query: 61  FSPHRLLHSYKRSFNGFVVKLSEAEAQKISEMEGVISVFRNGRKELHTTRSWDFMGLSQQ 120
           F+PH LLHSY+RSFNGFVVKL+E E + ISEM+GVISVF N +K+LHTTRSWDFMGLSQQ
Sbjct: 61  FAPHSLLHSYQRSFNGFVVKLTEDEVKIISEMKGVISVFPNEKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSIESDIIVGVFDSGIWPESPSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARS 180
             RVPS+ESDIIVGV D+GIWPESPSF D GY PPPP+WKG CE S NFSCNNKIIGARS
Sbjct: 121 VGRVPSVESDIIVGVLDTGIWPESPSFLDEGYGPPPPRWKGSCEASLNFSCNNKIIGARS 180

Query: 181 YRSDGLYSQTDLQSPRDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYK 240
           YR++G Y   D+Q PRDS+GHGTHTASTVAGGLVRQASMLGLG GTARGGVPSARIA+YK
Sbjct: 181 YRTNGQYPINDIQGPRDSNGHGTHTASTVAGGLVRQASMLGLGSGTARGGVPSARIASYK 240

Query: 241 ICWSDGCYDADILAAFDDAIADGVDIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSM 300
           ICWSD C DADILAAFDDAIADGVDIISFSVGG +PK YFNDS+AIGAFHAMKKGILTSM
Sbjct: 241 ICWSDDCSDADILAAFDDAIADGVDIISFSVGGHTPKDYFNDSMAIGAFHAMKKGILTSM 300

Query: 301 SAGNDGPDFFTIRNFSPWSLSVAASTTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPL 360
           SAGNDGP  FTIRNFSPWSLSVAASTT+R +L+G+QLGDG +F GVTINTFDLNGTQYPL
Sbjct: 301 SAGNDGPQSFTIRNFSPWSLSVAASTTNRSYLSGIQLGDGRSFNGVTINTFDLNGTQYPL 360

Query: 361 VYAGDIPNITAGFNASRSRFCLENSVDKELVRGKITLCDSILSPKELGSLKNAIGIIMQD 420
           VYAG+IPNIT GFN S SRFCL NSVD+E V+GKI LCD  +SPK LGSL+ AIGIIMQD
Sbjct: 361 VYAGNIPNITGGFNGSISRFCLPNSVDRESVKGKIVLCDFFVSPKNLGSLEGAIGIIMQD 420

Query: 421 RSPKDLTSSFPLPASHLGTQQG-LISSYINLTSLPTATILKSTEG--KTSTFVASFSSRG 480
            +PKDLT  FPLPASHLGTQ+G LISSY NLT LPTATILKSTEG  K + FVASFSSRG
Sbjct: 421 TNPKDLTFPFPLPASHLGTQEGALISSYANLTGLPTATILKSTEGKYKATPFVASFSSRG 480

Query: 481 PNPITLDILKPDLSGPGVEILAAWSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAA 540
           PNPIT DILKPDLSGPGVEILAAWSPIG PSGA++D R +L+NIISGTSMSCPHATAVAA
Sbjct: 481 PNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA 540

Query: 541 YVKSFHPSWSPAALKSALMTTAFPMKAELNSDAEFAYGSGHINPLSAINPGLIYNATEID 600
           YVK+FHPSWSPAALKSALMTTAFPM+A+LN DAEFAYGSGHINPLSA+NPGLIYNATEID
Sbjct: 541 YVKTFHPSWSPAALKSALMTTAFPMRADLNPDAEFAYGSGHINPLSAVNPGLIYNATEID 600

Query: 601 YIRFLCGQGYSTKQLQQVSEDNNTCNSANNFDTVFNLNYPSFALSTTISTPISQVYKRRV 660
           Y+RFLCGQGYSTK +QQVS DN++C S  + D VF+LNYPSFALST+IST ISQVY+RRV
Sbjct: 601 YVRFLCGQGYSTKLVQQVSGDNSSC-SRGDIDLVFDLNYPSFALSTSISTSISQVYRRRV 660

Query: 661 TNVGSANSTYKASIFAPLGLNIIVNPSVLSFKALGEEKNFEVTIEGSIDSGIASASLVWD 720
           TNVGSANSTYKA +  P GLNI VNPSVLSFKALGEE +FE+TIEGSI S IASASLVWD
Sbjct: 661 TNVGSANSTYKAIVSGPSGLNITVNPSVLSFKALGEELSFELTIEGSISSSIASASLVWD 720

Query: 721 DGLHKVRSPITVFDANTFV 728
           DG HKV+SPI VFD NTF+
Sbjct: 721 DGKHKVKSPIVVFDENTFI 738

BLAST of Sed0004524 vs. NCBI nr
Match: KAG6579374.1 (hypothetical protein SDJN03_23822, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 584/739 (79.03%), Postives = 643/739 (87.01%), Query Frame = 0

Query: 1   MLSLPKLLFLSFCFSSF---------QARTYIVYMGSHSKDKVSTSSHHMRMLQETIGST 60
           M SL +LLFL+FC S             +TYIVYMGSH K++V T SHH+RMLQETIGS+
Sbjct: 1   MSSLSRLLFLAFCLSQLFFSSNSEHDDRKTYIVYMGSHPKERVLTRSHHVRMLQETIGSS 60

Query: 61  FSPHRLLHSYKRSFNGFVVKLSEAEAQKISEMEGVISVFRNGRKELHTTRSWDFMGLSQQ 120
           F+PH LLHSY+RSFNGFV KL+E E QK+SEM+GVISVF NG+K+LHTTRSWDFMGLSQQ
Sbjct: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSIESDIIVGVFDSGIWPESPSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARS 180
           ASRVPS+ESDIIVGV D+GIWPESPSF D GY PPPPKWKG CEVS++FSCNNKIIGARS
Sbjct: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSCNNKIIGARS 180

Query: 181 YRSDGLYSQTDLQSPRDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYK 240
           YR++G Y   D+Q PRDS+GHGTH ASTVAG LVRQASMLGLG GTARGGVPSARIA+YK
Sbjct: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGVPSARIASYK 240

Query: 241 ICWSDGCYDADILAAFDDAIADGVDIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSM 300
           ICWSDGC DAD+LAAFDDAIADGVDIISFSVGG  P+ YFNDSIAIGAFHAMKKGILTSM
Sbjct: 241 ICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHAMKKGILTSM 300

Query: 301 SAGNDGPDFFTIRNFSPWSLSVAASTTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPL 360
           SAGN GP  FT+RNFSPWSLSVAASTTDR+ LTGVQLGDG +F GVT+NTFDLNGTQYPL
Sbjct: 301 SAGNSGPKSFTVRNFSPWSLSVAASTTDRKLLTGVQLGDGRSFDGVTVNTFDLNGTQYPL 360

Query: 361 VYAGDIPNITAGFNASRSRFCLENSVDKELVRGKITLCDSILSPKELGSLKNAIGIIMQD 420
           VYAG+IPN+  GFN S SRFCL NSVDKE V+GKI LCD  +SP  L  L+ AIGIIMQD
Sbjct: 361 VYAGNIPNV--GFNGSISRFCLTNSVDKESVKGKIVLCDFFISPTNLSFLEGAIGIIMQD 420

Query: 421 RSPKDLTSSFPLPASHLGTQQG-LISSYINLTSLPTATILKSTEG--KTSTFVASFSSRG 480
            +PKDLT  FPLPASHLGTQ+G LISSY NLTSLPTATILKSTEG  K + FVASFSSRG
Sbjct: 421 NNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG 480

Query: 481 PNPITLDILKPDLSGPGVEILAAWSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAA 540
           PNPIT DILKPDLSGPGVEILAAWSPIG PSGA++D R +L+NIISGTSMSCPHATAVAA
Sbjct: 481 PNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA 540

Query: 541 YVKSFHPSWSPAALKSALMTTAFPMKAELNSDAEFAYGSGHINPLSAINPGLIYNATEID 600
           YVKSFHPSWSPAALKSALMTTAFPM+++LN DAEFAYGSGHINPL A+NPGLIYNATEID
Sbjct: 541 YVKSFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEID 600

Query: 601 YIRFLCGQGYSTKQLQQVSEDNNTCNSANNFDTVFNLNYPSFALSTTISTPISQVYKRRV 660
           Y+RFLCGQGYSTK +QQVS DN++C S ++ D VF+LNYPSFALST IST ISQVY+R+V
Sbjct: 601 YVRFLCGQGYSTKLIQQVSGDNSSC-SRSDSDVVFDLNYPSFALSTPISTSISQVYRRKV 660

Query: 661 TNVGSANSTYKASIFAPLGLNIIVNPSVLSFKALGEEKNFEVTIEGSIDSGIASASLVWD 720
           TNVGSANS YKA++  P GL I VNPSVLSFKALGEE +FEVTIEGSI S IASASLVWD
Sbjct: 661 TNVGSANSIYKATVSGPSGLKITVNPSVLSFKALGEELSFEVTIEGSISSSIASASLVWD 720

Query: 721 DGLHKVRSPITVFDANTFV 728
           DG HKVRSPI VFD+N F+
Sbjct: 721 DGQHKVRSPIIVFDSNKFI 736

BLAST of Sed0004524 vs. NCBI nr
Match: XP_022922127.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 585/739 (79.16%), Postives = 644/739 (87.14%), Query Frame = 0

Query: 1   MLSLPKLLFLSFCFSSF---------QARTYIVYMGSHSKDKVSTSSHHMRMLQETIGST 60
           M SL +LLFL+FCFS             +TYIVYMGSH KD+V T SHH+RMLQETIGS 
Sbjct: 1   MSSLSRLLFLAFCFSQLFFCSNSEHDDRKTYIVYMGSHPKDRVLTRSHHVRMLQETIGSH 60

Query: 61  FSPHRLLHSYKRSFNGFVVKLSEAEAQKISEMEGVISVFRNGRKELHTTRSWDFMGLSQQ 120
           F+PH LLHSY+RSFNGFV KL+E E QK+SEM+GVISVF NG+K+LHTTRSWDFMGLSQQ
Sbjct: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSIESDIIVGVFDSGIWPESPSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARS 180
           ASRVPS+ESDIIVGV D+GIWPESPSF D GY PPP KWKG CEVS++FSCNNKIIGARS
Sbjct: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPRKWKGSCEVSSDFSCNNKIIGARS 180

Query: 181 YRSDGLYSQTDLQSPRDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYK 240
           YR++G Y   D+Q PRDS+GHGTH ASTVAGGLVRQASMLGLG GTARGGVPSARIA+YK
Sbjct: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGGLVRQASMLGLGSGTARGGVPSARIASYK 240

Query: 241 ICWSDGCYDADILAAFDDAIADGVDIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSM 300
           ICWSDGC DADILAAFDDAIADGVDIISFSVGG +P+ YFNDSIAIGAFHAMKK ILTSM
Sbjct: 241 ICWSDGCPDADILAAFDDAIADGVDIISFSVGGQTPRDYFNDSIAIGAFHAMKKEILTSM 300

Query: 301 SAGNDGPDFFTIRNFSPWSLSVAASTTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPL 360
           SAGN+GP  FT+RNFSPWSLSVAASTTDR+FLTGVQLGDG +F GVTINTFDLNGTQYPL
Sbjct: 301 SAGNNGPKSFTVRNFSPWSLSVAASTTDRKFLTGVQLGDGRSFNGVTINTFDLNGTQYPL 360

Query: 361 VYAGDIPNITAGFNASRSRFCLENSVDKELVRGKITLCDSILSPKELGSLKNAIGIIMQD 420
           VYAG+IPNI  GFN S SRFCL NSVDKE V+GKI LCD  + P  L  L+ AIGIIMQD
Sbjct: 361 VYAGNIPNI--GFNGSISRFCLTNSVDKESVKGKIVLCDFFVLPTNLNFLEGAIGIIMQD 420

Query: 421 RSPKDLTSSFPLPASHLGTQQG-LISSYINLTSLPTATILKSTEG--KTSTFVASFSSRG 480
            +PKDLT  FPLPASHLGTQ+G LISSY NLTSLPTATILKSTEG  K + FVASFSSRG
Sbjct: 421 NNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG 480

Query: 481 PNPITLDILKPDLSGPGVEILAAWSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAA 540
           PNPIT DILKPDLSGPGVEILAAWSPIG PSGA++D R +L+NIISGTSMSCPHATAVAA
Sbjct: 481 PNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA 540

Query: 541 YVKSFHPSWSPAALKSALMTTAFPMKAELNSDAEFAYGSGHINPLSAINPGLIYNATEID 600
           YVK+FHPSWSPAALKSALMTTAFPM+++LN DAEFAYGSGHINPL A+NPGLIYNATEID
Sbjct: 541 YVKTFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEID 600

Query: 601 YIRFLCGQGYSTKQLQQVSEDNNTCNSANNFDTVFNLNYPSFALSTTISTPISQVYKRRV 660
           Y+RFLCGQGYSTK +QQVS DN++C S ++ + VF+LNYPSFALST+ISTPISQVY+RRV
Sbjct: 601 YVRFLCGQGYSTKLIQQVSGDNSSC-SRSDSNVVFDLNYPSFALSTSISTPISQVYRRRV 660

Query: 661 TNVGSANSTYKASIFAPLGLNIIVNPSVLSFKALGEEKNFEVTIEGSIDSGIASASLVWD 720
           TNVGSANSTY A +  P  L I VNPSVLSFKALGEE +FEVTIEGSI SGI SASLVWD
Sbjct: 661 TNVGSANSTYNAIVSGPSRLKITVNPSVLSFKALGEELSFEVTIEGSISSGIVSASLVWD 720

Query: 721 DGLHKVRSPITVFDANTFV 728
           DG HKVRSP+ VFD+ +F+
Sbjct: 721 DGQHKVRSPVIVFDSTSFI 736

BLAST of Sed0004524 vs. NCBI nr
Match: KAG6579404.1 (hypothetical protein SDJN03_23852, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 582/738 (78.86%), Postives = 634/738 (85.91%), Query Frame = 0

Query: 1   MLSLPKLLFLSFCFSSF---------QARTYIVYMGSHSKDKVSTSSHHMRMLQETIGST 60
           M SL  LLFL+FC S              TYIVYMGSH KD+ ST +HH RML E IGS+
Sbjct: 1   MSSLSSLLFLAFCLSLLFLGSNSKDDDRTTYIVYMGSHPKDRGSTRAHHERMLGEAIGSS 60

Query: 61  FSPHRLLHSYKRSFNGFVVKLSEAEAQKISEMEGVISVFRNGRKELHTTRSWDFMGLSQQ 120
           F+P  LLHSYKRSFNGFV KL+E E QK+SEM+GVISVF NG+K+LHTTRSWDFMGLSQQ
Sbjct: 61  FAPRSLLHSYKRSFNGFVAKLTEEEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSIESDIIVGVFDSGIWPESPSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARS 180
            SRVPS+ESDIIVGV D+GIWP+SPSF D GY PPPPKWKG CE S NFSCNNKIIGARS
Sbjct: 121 VSRVPSVESDIIVGVLDTGIWPDSPSFLDEGYGPPPPKWKGSCEASLNFSCNNKIIGARS 180

Query: 181 YRSDGLYSQTDLQSPRDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYK 240
           YR+ G Y+  D+Q P DS+GHGTHTASTVAGGLVRQASMLGLG GTARGGVPSARIA+YK
Sbjct: 181 YRTSGNYAIGDIQGPIDSNGHGTHTASTVAGGLVRQASMLGLGTGTARGGVPSARIASYK 240

Query: 241 ICWSDGCYDADILAAFDDAIADGVDIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSM 300
           ICWSDGC +ADILAAFDDAIADGVDIIS SVGG +P  YFNDSIAIGAFHAMK GILTSM
Sbjct: 241 ICWSDGCPEADILAAFDDAIADGVDIISLSVGGHTPNNYFNDSIAIGAFHAMKNGILTSM 300

Query: 301 SAGNDGPDFFTIRNFSPWSLSVAASTTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPL 360
           SAGNDG   FTIRNFSPWSLSVAASTTDRRFL+ VQLGDG +F GVTINTFDLNGTQYPL
Sbjct: 301 SAGNDGWKPFTIRNFSPWSLSVAASTTDRRFLSKVQLGDGRSFNGVTINTFDLNGTQYPL 360

Query: 361 VYAGDIPNITAGFNASRSRFCLENSVDKELVRGKITLCDSILSPKELGSLKNAIGIIMQD 420
           VYAG+IPN+TAGFN S SRFC  NSVD++LV+GKI LCD  +SPK+   L+ AIGIIMQD
Sbjct: 361 VYAGNIPNVTAGFNGSVSRFCTRNSVDRDLVKGKIVLCDHFVSPKKTIFLEGAIGIIMQD 420

Query: 421 RSPKDLTSSFPLPASHLGTQQG-LISSYINLTSLPTATILKSTEG--KTSTFVASFSSRG 480
            +PKDL+S FPLPASHLGTQ+G LI SY NLTSLPTATILKSTEG  K + FVASFSSRG
Sbjct: 421 NNPKDLSSPFPLPASHLGTQEGALIFSYANLTSLPTATILKSTEGKYKATPFVASFSSRG 480

Query: 481 PNPITLDILKPDLSGPGVEILAAWSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAA 540
           PNPIT DILKPDLSGPGVEILAAW+P+G PSGAK+D R +L+NIISGTSM+CPHATAVAA
Sbjct: 481 PNPITPDILKPDLSGPGVEILAAWTPLGPPSGAKEDTRQLLFNIISGTSMACPHATAVAA 540

Query: 541 YVKSFHPSWSPAALKSALMTTAFPMKAELNSDAEFAYGSGHINPLSAINPGLIYNATEID 600
           YVKSFHPSWSPAALKSALMTTAFPM+AELN +AEFAYGSGHINPLSA+NPGLIYNATEID
Sbjct: 541 YVKSFHPSWSPAALKSALMTTAFPMRAELNPEAEFAYGSGHINPLSAVNPGLIYNATEID 600

Query: 601 YIRFLCGQGYSTKQLQQVSEDNNTCNSANNFDTVFNLNYPSFALSTTISTPISQVYKRRV 660
           Y+RFLCGQGYSTK +QQVS DN++C S  NFD VF+LNYPSFALST IST ISQVY+RRV
Sbjct: 601 YVRFLCGQGYSTKLVQQVSGDNSSC-SRGNFDLVFDLNYPSFALSTPISTSISQVYRRRV 660

Query: 661 TNVGSANSTYKASIFAPLGLNIIVNPSVLSFKALGEEKNFEVTIEGSIDSGIASASLVWD 720
           TNVGSANSTY A +  P GL I VNPSVL FKALGEE +FEVTIEGSI S IASASLVWD
Sbjct: 661 TNVGSANSTYNAIVSGPSGLKITVNPSVLWFKALGEELSFEVTIEGSISSSIASASLVWD 720

Query: 721 DGLHKVRSPITVFDANTF 727
           DG HKV+SPI VFD NTF
Sbjct: 721 DGHHKVKSPIVVFDPNTF 737

BLAST of Sed0004524 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 7.2e-234
Identity = 419/729 (57.48%), Postives = 521/729 (71.47%), Query Frame = 0

Query: 6   KLLFLSFCFSSFQA----------RTYIVYMGSHSKDKVSTSSHHMRMLQETIGSTFSPH 65
           KL F S  FS+  A            YIVYMG   +D  S   HH  ML++ +GSTF+P 
Sbjct: 8   KLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVGSTFAPE 67

Query: 66  RLLHSYKRSFNGFVVKLSEAEAQKISEMEGVISVFRNGRKELHTTRSWDFMGLSQQASRV 125
            +LH+YKRSFNGF VKL+E EA+KI+ MEGV+SVF N   ELHTTRSWDF+G      R 
Sbjct: 68  SVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRR 127

Query: 126 PSIESDIIVGVFDSGIWPESPSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARSYRSD 185
             +ES+I+VGV D+GIWPESPSF+D G+SPPPPKWKG CE S NF CN KIIGARSY   
Sbjct: 128 SQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIG 187

Query: 186 GLYSQTDLQSPRDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYKICWS 245
              S  D+  PRD++GHGTHTAST AGGLV QA++ GLGLGTARGGVP ARIAAYK+CW+
Sbjct: 188 RPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWN 247

Query: 246 DGCYDADILAAFDDAIADGVDIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSMSAGN 305
           DGC D DILAA+DDAIADGVDIIS SVGG +P++YF D+IAIG+FHA+++GILTS SAGN
Sbjct: 248 DGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGN 307

Query: 306 DGPDFFTIRNFSPWSLSVAASTTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPLVYAG 365
            GP+FFT  + SPW LSVAAST DR+F+T VQ+G+G +F GV+INTFD     YPLV   
Sbjct: 308 GGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGR 367

Query: 366 DIPNITAGFNASRSRFCLENSVDKELVRGKITLCDSILSPKE-LGSLKNAIGIIMQDRSP 425
           DIPN   GF+ S SRFC + SV+  L++GKI +C++   P E   SL  A G++M   + 
Sbjct: 368 DIPN--TGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNT- 427

Query: 426 KDLTSSFPLPASHLGTQQGLIS-SYINLTSLPTATILKSTE--GKTSTFVASFSSRGPNP 485
           +D   S+PLP+S L     L +  YI     P ATI KST     ++  V SFSSRGPN 
Sbjct: 428 RDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNR 487

Query: 486 ITLDILKPDLSGPGVEILAAWSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAAYVK 545
            T D++KPD+SGPGVEILAAW  +    G +   RN L+NIISGTSMSCPH T +A YVK
Sbjct: 488 ATKDVIKPDISGPGVEILAAWPSVAPVGGIR---RNTLFNIISGTSMSCPHITGIATYVK 547

Query: 546 SFHPSWSPAALKSALMTTAFPMKAELNSDAEFAYGSGHINPLSAINPGLIYNATEIDYIR 605
           +++P+WSPAA+KSALMTTA PM A  N  AEFAYGSGH+NPL A+ PGL+Y+A E DY++
Sbjct: 548 TYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVK 607

Query: 606 FLCGQGYSTKQLQQVSEDNNTCNSANNFDTVFNLNYPSFALSTTISTPISQVYKRRVTNV 665
           FLCGQGY+T+ +++++ D + C S N    V++LNYPSF LS + S   +Q + R +T+V
Sbjct: 608 FLCGQGYNTQAVRRITGDYSACTSGNT-GRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSV 667

Query: 666 GSANSTYKASIFAPLGLNIIVNPSVLSFKALGEEKNFEVTIEGSIDSGIASASLVWDDGL 721
               STY+A I AP GL I VNP+VLSF  LG+ K+F +T+ GSI   + SASLVW DG+
Sbjct: 668 APQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGV 727

BLAST of Sed0004524 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 1.7e-190
Identity = 358/709 (50.49%), Postives = 468/709 (66.01%), Query Frame = 0

Query: 22  YIVYMGSHSKDKVSTSSHHMRMLQETIGSTFSPHRLLHSYKRSFNGFVVKLSEAEAQKIS 81
           YIVYMG+  + K S  SHH+ +LQ+ +G+  + H L+ SYKRSFNGF   LS+AE+QK+ 
Sbjct: 33  YIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQ 92

Query: 82  EMEGVISVFRNGRKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSGIWPESPSFNDT 141
            M+ V+SVF +   EL TTRSWDF+G  ++A R    ESD+IVGV DSGIWPES SF+D 
Sbjct: 93  NMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDE 152

Query: 142 GYSPPPPKWKGICEVSANFSCNNKIIGARSYRSDGLYSQTDLQSPRDSDGHGTHTASTVA 201
           G+ PPP KWKG C+    F+CNNK+IGAR Y            S RD +GHGTHTAST A
Sbjct: 153 GFGPPPKKWKGSCKGGLKFACNNKLIGARFYNKFA-------DSARDEEGHGTHTASTAA 212

Query: 202 GGLVRQASMLGLGLGTARGGVPSARIAAYKICWSDGCYDADILAAFDDAIADGVDIISFS 261
           G  V+ AS  GL  GTARGGVPSARIAAYK+C+ + C D DILAAFDDAIADGVD+IS S
Sbjct: 213 GNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISIS 272

Query: 262 VGGGSPKYYFNDSIAIGAFHAMKKGILTSMSAGNDGPDFFTIRNFSPWSLSVAASTTDRR 321
           +         N S+AIG+FHAM +GI+T+ SAGN+GPD  ++ N SPW ++VAAS TDR+
Sbjct: 273 ISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQ 332

Query: 322 FLTGVQLGDGTNFTGVTINTFDLNGTQYPLVYAGDIPNITAGFNASRSRFCLENSVDKEL 381
           F+  V LG+G   TG+++NTF+LNGT++P+VY     N++   + +++ +C    VD EL
Sbjct: 333 FIDRVVLGNGKALTGISVNTFNLNGTKFPIVYG---QNVSRNCSQAQAGYCSSGCVDSEL 392

Query: 382 VRGKITLCDSILSPKELGSLKNAIGIIMQDRSPKDLTSSFPLPASHLGTQQ-GLISSYIN 441
           V+GKI LCD  L  +E   L  AIG+I+Q+    D     P PAS LG +    I SYI 
Sbjct: 393 VKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIE 452

Query: 442 LTSLPTATILKSTE--GKTSTFVASFSSRGPNPITLDILKPDLSGPGVEILAAWSPIGSP 501
               P A IL++ E   + + +V SFSSRGP+ +  ++LKPD+S PG+EILAA+SP+ SP
Sbjct: 453 SAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASP 512

Query: 502 SG--AKDDNRNILYNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAFPMKAE 561
           S     +D R++ Y+++SGTSM+CPH   VAAYVKSFHP WSP+A+KSA+MTTA PM  +
Sbjct: 513 SSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLK 572

Query: 562 LNSDAEFAYGSGHINPLSAINPGLIYNATEIDYIRFLCGQGYSTKQLQQVSEDNNTCNSA 621
            N + EFAYGSG INP  A +PGL+Y     DY++ LC +G+ +  L   S  N TC+  
Sbjct: 573 KNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSER 632

Query: 622 NNFDTVFNLNYPSFALSTTISTPISQVYKRRVTNVGSANSTYKASIFAPL--GLNIIVNP 681
                V +LNYP+     +   P +  +KR VTNVG  NSTYKAS+  PL   L I + P
Sbjct: 633 TE---VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASV-VPLQPELQISIEP 692

Query: 682 SVLSFKALGEEKNFEVTIEGS--IDSGIASASLVWDDGLHKVRSPITVF 722
            +L F  L E+K+F VTI G    D    S+S+VW DG H VRSPI  +
Sbjct: 693 EILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAY 725

BLAST of Sed0004524 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 2.0e-183
Identity = 354/738 (47.97%), Postives = 490/738 (66.40%), Query Frame = 0

Query: 1   MLSLPKLLFLSFCFS-SFQARTYIVYMGS-HSKDKVSTSSHHMRMLQETIGSTFSPHRLL 60
           +LS   +LFLS   + +   + YIVYMGS  S+   + +S HM +LQE  G +    RL+
Sbjct: 10  LLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSIEGRLV 69

Query: 61  HSYKRSFNGFVVKLSEAEAQKISEMEGVISVFRNGRKELHTTRSWDFMGLSQ--QASRVP 120
            SYKRSFNGF  +L+E+E +++++M GV+SVF N + +L TT SWDFMGL +  +  R P
Sbjct: 70  RSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNP 129

Query: 121 SIESDIIVGVFDSGIWPESPSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARSYRSDG 180
           ++ESD I+GV DSGI PES SF+D G+ PPP KWKG+C    NF+CNNK+IGAR Y S+G
Sbjct: 130 TVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTSEG 189

Query: 181 LYSQTDLQSPRDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYKICWSD 240
                     RD DGHGTHTAST AG  V  AS  G+G GT RGGVP++R+AAYK+C   
Sbjct: 190 ---------TRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPT 249

Query: 241 GCYDADILAAFDDAIADGVDIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSMSAGND 300
           GC    +L+AFDDAIADGVD+I+ S+G  +   + ND IAIGAFHAM KG+LT  SAGN 
Sbjct: 250 GCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNS 309

Query: 301 GPDFFTIRNFSPWSLSVAASTTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPLVYAGD 360
           GP   ++   +PW L+VAASTT+R F+T V LG+G    G ++N +++ G  YPLVY   
Sbjct: 310 GPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKS 369

Query: 361 IPNITAGFNASRSRFCLENSVDKELVRGKITLCDSILSPKELGSLKNAIGIIMQDRSPKD 420
               ++  +A  +  C  + VDK  V+GKI +C      K + S+  A+G+I +   P D
Sbjct: 370 A--ASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV-GAVGLIYRTPKP-D 429

Query: 421 LTSSFPLPASHLGTQQ-GLISSYINLTSLPTATILKSTE--GKTSTFVASFSSRGPNPIT 480
           +    PLPA+ L T+    + SY+  T  P A +LK+     +TS  +ASFSSRGPN I 
Sbjct: 430 VAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIA 489

Query: 481 LDILKPDLSGPGVEILAAWSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAAYVKSF 540
           +DILKPD++ PGVEILAA+SP G PS  +DD R++ Y+++SGTSMSCPH   VAAYVK+F
Sbjct: 490 VDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTF 549

Query: 541 HPSWSPAALKSALMTTAFPMKAELN--SDAEFAYGSGHINPLSAINPGLIYNATEIDYIR 600
           +P WSP+ ++SA+MTTA+P+ A     +  EFAYGSGH++P++A NPGL+Y   + D+I 
Sbjct: 550 NPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIA 609

Query: 601 FLCGQGYSTKQLQQVSEDNNTCNSANNFDTVFNLNYPSFALSTTIS-TPISQVYKRRVTN 660
           FLCG  Y+++ L+ +S +  TC+ A       NLNYPS +   + S T  +  + R +TN
Sbjct: 610 FLCGMNYTSQVLKVISGETVTCSEAKKI-LPRNLNYPSMSAKLSGSGTTFTVTFNRTLTN 669

Query: 661 VGSANSTYKASIFAPLG--LNIIVNPSVLSFKALGEEKNFEVTIEGS-IDSGI-ASASLV 720
           VG+ NSTY + + A  G  L++ + PSVLSFK + E+++F VT+ GS +DS + +SA+L+
Sbjct: 670 VGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLI 729

Query: 721 WDDGLHKVRSPITVFDAN 725
           W DG H VRSPI V+ ++
Sbjct: 730 WSDGTHNVRSPIVVYTSD 731

BLAST of Sed0004524 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 3.4e-183
Identity = 355/718 (49.44%), Postives = 475/718 (66.16%), Query Frame = 0

Query: 20  RTYIVYMGSHSK--DKVSTSSHHMRMLQETIGSTFSPHRLLHSYKRSFNGFVVKLSEAEA 79
           + YIVYMGS S   D + TS  HM +LQ+  G +    RL+ SYKRSFNGF  +L+E+E 
Sbjct: 31  QVYIVYMGSLSSRADYIPTSD-HMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESER 90

Query: 80  QKISEMEGVISVFRNGRKELHTTRSWDFMGLSQ--QASRVPSIESDIIVGVFDSGIWPES 139
             I+E+EGV+SVF N   +LHTT SWDFMG+ +     R  +IESD I+GV D+GIWPES
Sbjct: 91  TLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPES 150

Query: 140 PSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARSYRSDGLYSQTDLQSPRDSDGHGTH 199
            SF+D G+ PPP KWKG+C    NF+CNNK+IGAR Y S+G          RD+ GHGTH
Sbjct: 151 KSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSEG---------TRDTSGHGTH 210

Query: 200 TASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYKICWSDGCYDADILAAFDDAIADGV 259
           TAST AG  V+  S  G+G GT RGGVP++RIAAYK+C   GC    +L++FDDAIADGV
Sbjct: 211 TASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGV 270

Query: 260 DIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSMSAGNDGPDFFTIRNFSPWSLSVAA 319
           D+I+ S+G   P  + +D IAIGAFHAM KGILT  SAGN GP   T+ + +PW  +VAA
Sbjct: 271 DLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAA 330

Query: 320 STTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPLVYAGDIPNITAGFNASRSRFCLEN 379
           STT+R F+T V LG+G    G ++N FD+ G +YPLVY       ++  +A  +  C   
Sbjct: 331 STTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSA--ASSACDAKTAALCAPA 390

Query: 380 SVDKELVRGKITLCDSILSPKELGSLKNAIGIIMQDRSPK-DLTSSFPLPASHLGTQQ-G 439
            ++K  V+GKI +C     P      K+   I + D+SP+ D+  +  LPAS L  +   
Sbjct: 391 CLNKSRVKGKILVCG---GPSGYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFK 450

Query: 440 LISSYINLTSLPTATILKSTE--GKTSTFVASFSSRGPNPITLDILKPDLSGPGVEILAA 499
            + SYI     P A +LK+     +TS  +ASFSSRGPN I +DILKPD++ PGVEILAA
Sbjct: 451 SLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAA 510

Query: 500 WSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAF 559
           +SP G PS  +DD R + Y++ SGTSM+CPH   VAAYVK+F+P WSP+ ++SA+MTTA+
Sbjct: 511 FSPNGEPS--EDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAW 570

Query: 560 PMKAELN--SDAEFAYGSGHINPLSAINPGLIYNATEIDYIRFLCGQGYSTKQLQQVSED 619
           P+KA+    +  EFAYG+GH++P++A+NPGL+Y   + D+I FLCG  Y++K L+ +S D
Sbjct: 571 PVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGD 630

Query: 620 NNTCNSANNFDTVFNLNYPSFA--LSTTISTPISQVYKRRVTNVGSANSTYKASIFAPLG 679
              C+  N      NLNYPS +  LS T ST  S  + R +TNVG+ NSTYK+ + A  G
Sbjct: 631 TVKCSKKNKI-LPRNLNYPSMSAKLSGTDST-FSVTFNRTLTNVGTPNSTYKSKVVAGHG 690

Query: 680 --LNIIVNPSVLSFKALGEEKNFEVTIEGS-IDSGI-ASASLVWDDGLHKVRSPITVF 722
             L+I V PSVL FK + E+++F VT+ GS +DS + +SA+L+W DG H VRSPI V+
Sbjct: 691 SKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVY 729

BLAST of Sed0004524 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 3.5e-180
Identity = 342/717 (47.70%), Postives = 472/717 (65.83%), Query Frame = 0

Query: 20  RTYIVYMGS-HSKDKVSTSSHHMRMLQETIGSTFSPHRLLHSYKRSFNGFVVKLSEAEAQ 79
           + YIVY+GS  S+++ +  S HM +LQE  G +   +RL+ SYK+SFNGF  +L+E+E +
Sbjct: 33  QVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKKSFNGFAARLTESERK 92

Query: 80  KISEMEGVISVFRNGRKELHTTRSWDFMGLSQ--QASRVPSIESDIIVGVFDSGIWPESP 139
           +++ ME V+SVF + + +L TT SW+FMGL +  +  R  SIESD I+GV DSGI+PES 
Sbjct: 93  RLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESD 152

Query: 140 SFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARSYRSDGLYSQTDLQSPRDSDGHGTHT 199
           SF+D G+ PPP KWKG C    NF+CNNK+IGAR Y +    +QT     RD  GHGTHT
Sbjct: 153 SFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKSKANQT----ARDYSGHGTHT 212

Query: 200 ASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYKICWSDGCYDADILAAFDDAIADGVD 259
           AS  AG  V  ++  GLG GTARGGVP+ARIA YK+C ++GC    +++AFDDAIADGVD
Sbjct: 213 ASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGEAMMSAFDDAIADGVD 272

Query: 260 IISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSMSAGNDGPDFFTIRNFSPWSLSVAAS 319
           +IS S+   +   +  D IAIGAFHAM  G+LT  +AGN+GP   T+ + +PW  SVAAS
Sbjct: 273 VISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWVFSVAAS 332

Query: 320 TTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPLVYAGDIPNITAGFNASRSRFCLENS 379
            T+R F+  V LGDG    G ++NT+D+NGT YPLVY       T   +  ++R C    
Sbjct: 333 VTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAALSTC--SVDKARLCEPKC 392

Query: 380 VDKELVRGKITLCDS---ILSPKELGSLKNAIGIIMQDRSP-KDLTSSFPLPASHLGTQQ 439
           +D +LV+GKI LCDS   ++  ++LG    A+G I+++  P +    SFP+        +
Sbjct: 393 LDGKLVKGKIVLCDSTKGLIEAQKLG----AVGSIVKNPEPDRAFIRSFPVSFLSNDDYK 452

Query: 440 GLISSYINLTSLPTATILKSTE--GKTSTFVASFSSRGPNPITLDILKPDLSGPGVEILA 499
            L+ SY+N T  P AT+LKS E   + +  VASFSSRGP+ I  DILKPD++ PGVEILA
Sbjct: 453 SLV-SYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILKPDITAPGVEILA 512

Query: 500 AWSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTA 559
           A+SP  SP+ ++ D R + Y+++SGTSM+CPH   VAAYVK+FHP WSP+ ++SA+MTTA
Sbjct: 513 AYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTA 572

Query: 560 FPMKAELNS--DAEFAYGSGHINPLSAINPGLIYNATEIDYIRFLCGQGYSTKQLQQVSE 619
           +PM A  +     EFAYGSGH++P+ AINPGL+Y  T+ D+I FLCG  Y++  L+ +S 
Sbjct: 573 WPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGLNYTSDHLRIISG 632

Query: 620 DNNTCNSANNFDTVFNLNYPSFALSTTISTPISQVYKRRVTNVGSANSTYKASI--FAPL 679
           DN+TC    +     NLNYP+ +   + + P +  ++R VTNVG   STY A +  F   
Sbjct: 633 DNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGS 692

Query: 680 GLNIIVNPSVLSFKALGEEKNFEVTIEGSI--DSGIASASLVWDDGLHKVRSPITVF 722
            L+I V+P VLS K++ E+++F VT+           SA+L+W DG H VRSPI V+
Sbjct: 693 KLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPVSANLIWSDGTHNVRSPIIVY 738

BLAST of Sed0004524 vs. ExPASy TrEMBL
Match: A0A6J1E2G4 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430231 PE=3 SV=1)

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 590/739 (79.84%), Postives = 646/739 (87.42%), Query Frame = 0

Query: 1   MLSLPKLLFLSFCFSSF---------QARTYIVYMGSHSKDKVSTSSHHMRMLQETIGST 60
           M SL +LLFL+F  S             RTYIVYMGSH KD+VST SHH+RMLQETIGS+
Sbjct: 1   MSSLSRLLFLAFWLSRLFFSSNSEDDDKRTYIVYMGSHPKDRVSTRSHHVRMLQETIGSS 60

Query: 61  FSPHRLLHSYKRSFNGFVVKLSEAEAQKISEMEGVISVFRNGRKELHTTRSWDFMGLSQQ 120
           F+PH LLHSY+RSFNGFVVKL+E E + ISEM+GVISVF N +K+LHTTRSWDFMGLSQQ
Sbjct: 61  FAPHSLLHSYQRSFNGFVVKLTEDEVKIISEMKGVISVFPNEKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSIESDIIVGVFDSGIWPESPSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARS 180
             RVPS+ESDIIVGV D+GIWPESPSF D GY PPPP+WKG CE S NFSCNNKIIGARS
Sbjct: 121 VGRVPSVESDIIVGVLDTGIWPESPSFLDEGYGPPPPRWKGSCEASLNFSCNNKIIGARS 180

Query: 181 YRSDGLYSQTDLQSPRDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYK 240
           YR++G Y   D+Q PRDS+GHGTHTASTVAGGLVRQASMLGLG GTARGGVPSARIA+YK
Sbjct: 181 YRTNGQYPINDIQGPRDSNGHGTHTASTVAGGLVRQASMLGLGSGTARGGVPSARIASYK 240

Query: 241 ICWSDGCYDADILAAFDDAIADGVDIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSM 300
           ICWSD C DADILAAFDDAIADGVDIISFSVGG +PK YFNDS+AIGAFHAMKKGILTSM
Sbjct: 241 ICWSDDCSDADILAAFDDAIADGVDIISFSVGGHTPKDYFNDSMAIGAFHAMKKGILTSM 300

Query: 301 SAGNDGPDFFTIRNFSPWSLSVAASTTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPL 360
           SAGNDGP  FTIRNFSPWSLSVAASTT+R +L+G+QLGDG +F GVTINTFDLNGTQYPL
Sbjct: 301 SAGNDGPQSFTIRNFSPWSLSVAASTTNRSYLSGIQLGDGRSFNGVTINTFDLNGTQYPL 360

Query: 361 VYAGDIPNITAGFNASRSRFCLENSVDKELVRGKITLCDSILSPKELGSLKNAIGIIMQD 420
           VYAG+IPNIT GFN S SRFCL NSVD+E V+GKI LCD  +SPK LGSL+ AIGIIMQD
Sbjct: 361 VYAGNIPNITGGFNGSISRFCLPNSVDRESVKGKIVLCDFFVSPKNLGSLEGAIGIIMQD 420

Query: 421 RSPKDLTSSFPLPASHLGTQQG-LISSYINLTSLPTATILKSTEG--KTSTFVASFSSRG 480
            +PKDLT  FPLPASHLGTQ+G LISSY NLT LPTATILKSTEG  K + FVASFSSRG
Sbjct: 421 TNPKDLTFPFPLPASHLGTQEGALISSYANLTGLPTATILKSTEGKYKATPFVASFSSRG 480

Query: 481 PNPITLDILKPDLSGPGVEILAAWSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAA 540
           PNPIT DILKPDLSGPGVEILAAWSPIG PSGA++D R +L+NIISGTSMSCPHATAVAA
Sbjct: 481 PNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA 540

Query: 541 YVKSFHPSWSPAALKSALMTTAFPMKAELNSDAEFAYGSGHINPLSAINPGLIYNATEID 600
           YVK+FHPSWSPAALKSALMTTAFPM+A+LN DAEFAYGSGHINPLSA+NPGLIYNATEID
Sbjct: 541 YVKTFHPSWSPAALKSALMTTAFPMRADLNPDAEFAYGSGHINPLSAVNPGLIYNATEID 600

Query: 601 YIRFLCGQGYSTKQLQQVSEDNNTCNSANNFDTVFNLNYPSFALSTTISTPISQVYKRRV 660
           Y+RFLCGQGYSTK +QQVS DN++C S  + D VF+LNYPSFALST+IST ISQVY+RRV
Sbjct: 601 YVRFLCGQGYSTKLVQQVSGDNSSC-SRGDIDLVFDLNYPSFALSTSISTSISQVYRRRV 660

Query: 661 TNVGSANSTYKASIFAPLGLNIIVNPSVLSFKALGEEKNFEVTIEGSIDSGIASASLVWD 720
           TNVGSANSTYKA +  P GLNI VNPSVLSFKALGEE +FE+TIEGSI S IASASLVWD
Sbjct: 661 TNVGSANSTYKAIVSGPSGLNITVNPSVLSFKALGEELSFELTIEGSISSSIASASLVWD 720

Query: 721 DGLHKVRSPITVFDANTFV 728
           DG HKV+SPI VFD NTF+
Sbjct: 721 DGKHKVKSPIVVFDENTFI 738

BLAST of Sed0004524 vs. ExPASy TrEMBL
Match: A0A6J1E2C5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430194 PE=3 SV=1)

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 585/739 (79.16%), Postives = 644/739 (87.14%), Query Frame = 0

Query: 1   MLSLPKLLFLSFCFSSF---------QARTYIVYMGSHSKDKVSTSSHHMRMLQETIGST 60
           M SL +LLFL+FCFS             +TYIVYMGSH KD+V T SHH+RMLQETIGS 
Sbjct: 1   MSSLSRLLFLAFCFSQLFFCSNSEHDDRKTYIVYMGSHPKDRVLTRSHHVRMLQETIGSH 60

Query: 61  FSPHRLLHSYKRSFNGFVVKLSEAEAQKISEMEGVISVFRNGRKELHTTRSWDFMGLSQQ 120
           F+PH LLHSY+RSFNGFV KL+E E QK+SEM+GVISVF NG+K+LHTTRSWDFMGLSQQ
Sbjct: 61  FAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSIESDIIVGVFDSGIWPESPSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARS 180
           ASRVPS+ESDIIVGV D+GIWPESPSF D GY PPP KWKG CEVS++FSCNNKIIGARS
Sbjct: 121 ASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPRKWKGSCEVSSDFSCNNKIIGARS 180

Query: 181 YRSDGLYSQTDLQSPRDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYK 240
           YR++G Y   D+Q PRDS+GHGTH ASTVAGGLVRQASMLGLG GTARGGVPSARIA+YK
Sbjct: 181 YRTNGQYPINDIQGPRDSNGHGTHAASTVAGGLVRQASMLGLGSGTARGGVPSARIASYK 240

Query: 241 ICWSDGCYDADILAAFDDAIADGVDIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSM 300
           ICWSDGC DADILAAFDDAIADGVDIISFSVGG +P+ YFNDSIAIGAFHAMKK ILTSM
Sbjct: 241 ICWSDGCPDADILAAFDDAIADGVDIISFSVGGQTPRDYFNDSIAIGAFHAMKKEILTSM 300

Query: 301 SAGNDGPDFFTIRNFSPWSLSVAASTTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPL 360
           SAGN+GP  FT+RNFSPWSLSVAASTTDR+FLTGVQLGDG +F GVTINTFDLNGTQYPL
Sbjct: 301 SAGNNGPKSFTVRNFSPWSLSVAASTTDRKFLTGVQLGDGRSFNGVTINTFDLNGTQYPL 360

Query: 361 VYAGDIPNITAGFNASRSRFCLENSVDKELVRGKITLCDSILSPKELGSLKNAIGIIMQD 420
           VYAG+IPNI  GFN S SRFCL NSVDKE V+GKI LCD  + P  L  L+ AIGIIMQD
Sbjct: 361 VYAGNIPNI--GFNGSISRFCLTNSVDKESVKGKIVLCDFFVLPTNLNFLEGAIGIIMQD 420

Query: 421 RSPKDLTSSFPLPASHLGTQQG-LISSYINLTSLPTATILKSTEG--KTSTFVASFSSRG 480
            +PKDLT  FPLPASHLGTQ+G LISSY NLTSLPTATILKSTEG  K + FVASFSSRG
Sbjct: 421 NNPKDLTFPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG 480

Query: 481 PNPITLDILKPDLSGPGVEILAAWSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAA 540
           PNPIT DILKPDLSGPGVEILAAWSPIG PSGA++D R +L+NIISGTSMSCPHATAVAA
Sbjct: 481 PNPITPDILKPDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA 540

Query: 541 YVKSFHPSWSPAALKSALMTTAFPMKAELNSDAEFAYGSGHINPLSAINPGLIYNATEID 600
           YVK+FHPSWSPAALKSALMTTAFPM+++LN DAEFAYGSGHINPL A+NPGLIYNATEID
Sbjct: 541 YVKTFHPSWSPAALKSALMTTAFPMRSDLNPDAEFAYGSGHINPLGAVNPGLIYNATEID 600

Query: 601 YIRFLCGQGYSTKQLQQVSEDNNTCNSANNFDTVFNLNYPSFALSTTISTPISQVYKRRV 660
           Y+RFLCGQGYSTK +QQVS DN++C S ++ + VF+LNYPSFALST+ISTPISQVY+RRV
Sbjct: 601 YVRFLCGQGYSTKLIQQVSGDNSSC-SRSDSNVVFDLNYPSFALSTSISTPISQVYRRRV 660

Query: 661 TNVGSANSTYKASIFAPLGLNIIVNPSVLSFKALGEEKNFEVTIEGSIDSGIASASLVWD 720
           TNVGSANSTY A +  P  L I VNPSVLSFKALGEE +FEVTIEGSI SGI SASLVWD
Sbjct: 661 TNVGSANSTYNAIVSGPSRLKITVNPSVLSFKALGEELSFEVTIEGSISSGIVSASLVWD 720

Query: 721 DGLHKVRSPITVFDANTFV 728
           DG HKVRSP+ VFD+ +F+
Sbjct: 721 DGQHKVRSPVIVFDSTSFI 736

BLAST of Sed0004524 vs. ExPASy TrEMBL
Match: A0A6J1IAA2 (cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111471532 PE=3 SV=1)

HSP 1 Score: 1138.6 bits (2944), Expect = 0.0e+00
Identity = 582/738 (78.86%), Postives = 635/738 (86.04%), Query Frame = 0

Query: 1   MLSLPKLLFLSFCFSSF---------QARTYIVYMGSHSKDKVSTSSHHMRMLQETIGST 60
           M SL +LLFL+F  S              TYIVYMGSH KD+VST SHH RML+E IGS+
Sbjct: 1   MSSLSRLLFLAFFLSLLFFASNSEHDNRTTYIVYMGSHPKDRVSTRSHHERMLEEAIGSS 60

Query: 61  FSPHRLLHSYKRSFNGFVVKLSEAEAQKISEMEGVISVFRNGRKELHTTRSWDFMGLSQQ 120
           F+   LLHSYK+SFNGFVVKL+E E QK+SEM+GVISVF NG+K+LHTTRSWDFMGLSQQ
Sbjct: 61  FTRGSLLHSYKKSFNGFVVKLTEDEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSIESDIIVGVFDSGIWPESPSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARS 180
            SRVP +ESDIIVGV D+GIWPESPSF D GY PPPPKWKG CE S NFSCNNKIIGARS
Sbjct: 121 VSRVPLVESDIIVGVLDTGIWPESPSFLDEGYGPPPPKWKGSCETSLNFSCNNKIIGARS 180

Query: 181 YRSDGLYSQTDLQSPRDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYK 240
           YR+ G Y+  D+Q P DS+GHGTHTASTVAGGLVRQASMLGLG GTARGGVPSARIA+YK
Sbjct: 181 YRTSGNYAIGDIQGPIDSNGHGTHTASTVAGGLVRQASMLGLGTGTARGGVPSARIASYK 240

Query: 241 ICWSDGCYDADILAAFDDAIADGVDIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSM 300
           ICWSDGC DADILAAFDDAIADGVDIISFSVGG +P  YFNDSIAIGAFHAMK GILTSM
Sbjct: 241 ICWSDGCSDADILAAFDDAIADGVDIISFSVGGHTPNNYFNDSIAIGAFHAMKNGILTSM 300

Query: 301 SAGNDGPDFFTIRNFSPWSLSVAASTTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPL 360
           SAGNDG   FTIRNFSPWSLSVAASTTDRRFL+ VQLGDG +F GVTINTFDLNGTQYPL
Sbjct: 301 SAGNDGWKPFTIRNFSPWSLSVAASTTDRRFLSKVQLGDGRSFDGVTINTFDLNGTQYPL 360

Query: 361 VYAGDIPNITAGFNASRSRFCLENSVDKELVRGKITLCDSILSPKELGSLKNAIGIIMQD 420
           VYAG+IPN++AGFN S SRFCL NSVD +LV+GKI LCD  +SPK+   L+ AIGIIMQD
Sbjct: 361 VYAGNIPNVSAGFNGSISRFCLRNSVDSDLVKGKIVLCDHFVSPKKTIFLEGAIGIIMQD 420

Query: 421 RSPKDLTSSFPLPASHLGTQQG-LISSYINLTSLPTATILKSTEG--KTSTFVASFSSRG 480
             PKDLT  FPLPASHLGTQQG LISSY NLTSLPTATILKSTEG  K + FVASFSSRG
Sbjct: 421 NRPKDLTFPFPLPASHLGTQQGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG 480

Query: 481 PNPITLDILKPDLSGPGVEILAAWSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAA 540
           PNPIT DILKPDLSGPGVEILAAW+P+G PSGA++D R +L+NIISGTSMSCPHATAVAA
Sbjct: 481 PNPITPDILKPDLSGPGVEILAAWTPLGPPSGAEEDTRQLLFNIISGTSMSCPHATAVAA 540

Query: 541 YVKSFHPSWSPAALKSALMTTAFPMKAELNSDAEFAYGSGHINPLSAINPGLIYNATEID 600
           YVKSFHPSWSPAALKSALMTTAFPM+A+LN DAEFAYGSGHINPL A+NPGLIYNATEID
Sbjct: 541 YVKSFHPSWSPAALKSALMTTAFPMRADLNPDAEFAYGSGHINPLGAVNPGLIYNATEID 600

Query: 601 YIRFLCGQGYSTKQLQQVSEDNNTCNSANNFDTVFNLNYPSFALSTTISTPISQVYKRRV 660
           Y+RFLCGQGYSTK +QQVS DN++C S  +FD VF+LNYPSFALST +ST ISQVY+RRV
Sbjct: 601 YVRFLCGQGYSTKLVQQVSGDNSSC-SRGDFDLVFDLNYPSFALSTPMSTSISQVYRRRV 660

Query: 661 TNVGSANSTYKASIFAPLGLNIIVNPSVLSFKALGEEKNFEVTIEGSIDSGIASASLVWD 720
           TNVGSANSTYKA +  P GL I +NPSVLSFKALGEE +FE+TIEGSI  GIASASLVWD
Sbjct: 661 TNVGSANSTYKAIVSGPSGLKITLNPSVLSFKALGEELSFELTIEGSISRGIASASLVWD 720

Query: 721 DGLHKVRSPITVFDANTF 727
           DG H V+SPI VFD NTF
Sbjct: 721 DGQHNVKSPIVVFDPNTF 737

BLAST of Sed0004524 vs. ExPASy TrEMBL
Match: A0A6J1E6I3 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430414 PE=3 SV=1)

HSP 1 Score: 1136.3 bits (2938), Expect = 0.0e+00
Identity = 580/738 (78.59%), Postives = 633/738 (85.77%), Query Frame = 0

Query: 1   MLSLPKLLFLSFCFSSF---------QARTYIVYMGSHSKDKVSTSSHHMRMLQETIGST 60
           M SL  LLFL+FC S              TYIVYMGSH KD+ ST +HH RML E IGS+
Sbjct: 1   MSSLSSLLFLAFCLSLLFLGSNSKDDDRTTYIVYMGSHPKDRGSTRAHHERMLGEAIGSS 60

Query: 61  FSPHRLLHSYKRSFNGFVVKLSEAEAQKISEMEGVISVFRNGRKELHTTRSWDFMGLSQQ 120
           F+PH LLHSYKRSFNGFV KL+E E QK+SEM+GVISVF NG+K+LHTTRSWDFMGLSQQ
Sbjct: 61  FAPHSLLHSYKRSFNGFVAKLTEEEVQKVSEMKGVISVFPNGKKQLHTTRSWDFMGLSQQ 120

Query: 121 ASRVPSIESDIIVGVFDSGIWPESPSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARS 180
            SRVPS+ESDIIVGV D+GIWP SPSF D GY PPPPKWKG CE S NFSCNNKIIGARS
Sbjct: 121 VSRVPSVESDIIVGVLDTGIWPNSPSFLDEGYGPPPPKWKGSCEASLNFSCNNKIIGARS 180

Query: 181 YRSDGLYSQTDLQSPRDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYK 240
           YR+ G Y+  D+Q P DS+GHGTHTASTVAGGLVRQASMLGLG GTARGGVPSARIA+YK
Sbjct: 181 YRTSGNYAIGDIQGPIDSNGHGTHTASTVAGGLVRQASMLGLGTGTARGGVPSARIASYK 240

Query: 241 ICWSDGCYDADILAAFDDAIADGVDIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSM 300
           ICWSDGC +ADILAAFDD IADGVDIIS SVGG +P  YFNDSIAIGAFHAMK GILTSM
Sbjct: 241 ICWSDGCPEADILAAFDDVIADGVDIISLSVGGHTPNNYFNDSIAIGAFHAMKNGILTSM 300

Query: 301 SAGNDGPDFFTIRNFSPWSLSVAASTTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPL 360
           SAGNDG   FTIRNFSPWSLSVA+STT+RRFL+ VQLGDG +F GVTINTFDLNGTQY L
Sbjct: 301 SAGNDGWKPFTIRNFSPWSLSVASSTTNRRFLSKVQLGDGRSFHGVTINTFDLNGTQYSL 360

Query: 361 VYAGDIPNITAGFNASRSRFCLENSVDKELVRGKITLCDSILSPKELGSLKNAIGIIMQD 420
           VYAG+IPN+TAGFN S SRFC  NSVD++LV+GKI LCD  +SPK+   L+ AIGIIMQD
Sbjct: 361 VYAGNIPNVTAGFNGSVSRFCTRNSVDRDLVKGKIVLCDHFVSPKKTIFLEGAIGIIMQD 420

Query: 421 RSPKDLTSSFPLPASHLGTQQG-LISSYINLTSLPTATILKSTEG--KTSTFVASFSSRG 480
            +PKDL+S FPLPASHLGTQ+G LISSY NLTSLPTATILKSTEG  K + FVASFSSRG
Sbjct: 421 NNPKDLSSPFPLPASHLGTQEGALISSYANLTSLPTATILKSTEGKYKATPFVASFSSRG 480

Query: 481 PNPITLDILKPDLSGPGVEILAAWSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAA 540
           PNPIT DILKPDLSGPGVEILAAW+P+G PSGAK+D R +L+NIISGTSMSCPHATAVAA
Sbjct: 481 PNPITPDILKPDLSGPGVEILAAWTPLGPPSGAKEDTRQLLFNIISGTSMSCPHATAVAA 540

Query: 541 YVKSFHPSWSPAALKSALMTTAFPMKAELNSDAEFAYGSGHINPLSAINPGLIYNATEID 600
           YVKSFHPSWSPAALKSALMTTAFPM+AELN +AEFAYGSGHINPLSA+NPGLIYNATEID
Sbjct: 541 YVKSFHPSWSPAALKSALMTTAFPMRAELNPEAEFAYGSGHINPLSAVNPGLIYNATEID 600

Query: 601 YIRFLCGQGYSTKQLQQVSEDNNTCNSANNFDTVFNLNYPSFALSTTISTPISQVYKRRV 660
           Y+RFLCGQGYSTK +QQVS DN++C S  +FD VF+LNYPSFALST IST ISQVY+RRV
Sbjct: 601 YVRFLCGQGYSTKLVQQVSGDNSSC-SRGDFDLVFDLNYPSFALSTPISTSISQVYRRRV 660

Query: 661 TNVGSANSTYKASIFAPLGLNIIVNPSVLSFKALGEEKNFEVTIEGSIDSGIASASLVWD 720
           TNVGSANSTY A +  P GL I VNPSVLSF ALGEE +FEVTIEGSI S IASASLVWD
Sbjct: 661 TNVGSANSTYNAIVSGPSGLKITVNPSVLSFNALGEELSFEVTIEGSISSSIASASLVWD 720

Query: 721 DGLHKVRSPITVFDANTF 727
           DG HKV+SPI VFD NTF
Sbjct: 721 DGHHKVKSPIVVFDPNTF 737

BLAST of Sed0004524 vs. ExPASy TrEMBL
Match: A0A1S3AUC4 (cucumisin-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1)

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 560/745 (75.17%), Postives = 624/745 (83.76%), Query Frame = 0

Query: 1   MLSLPKLLFLSFCFSSF-----------QARTYIVYMGSHSKDKVS----TSSHHMRMLQ 60
           M SL +LLFL FCFS               +TYIVYMGSH KD+VS    +S HHMR+LQ
Sbjct: 1   MSSLSRLLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLLQ 60

Query: 61  ETIGSTFSPHRLLHSYKRSFNGFVVKLSEAEAQKISEMEGVISVFRNGRKELHTTRSWDF 120
           E IGSTF+PH LLHSYKRSFNGFV KL+E EA+K+SEMEGVISVF N   +LHTTRSWDF
Sbjct: 61  EAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWDF 120

Query: 121 MGLSQQASRVPSIESDIIVGVFDSGIWPESPSFNDTGYSPPPPKWKGICEVSANFSCNNK 180
           MG S+Q  RVPS+ESDIIVGVFD+GIWPESPSF D GY PPPPKWKG CEVS NFSCNNK
Sbjct: 121 MGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNNK 180

Query: 181 IIGARSYRSDGLYSQTDLQSPRDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSA 240
           IIGA+SYRSDG Y   D++ PRDS+GHGTH ASTVAGGLVRQASMLGLG GTARGGVPSA
Sbjct: 181 IIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPSA 240

Query: 241 RIAAYKICWSDGCYDADILAAFDDAIADGVDIISFSVGGGSP-KYYFNDSIAIGAFHAMK 300
           RIAAYK+CWSD C  AD+LAAFDDAIADGVDIIS SVG   P   YFND IAIG FHAM+
Sbjct: 241 RIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAMR 300

Query: 301 KGILTSMSAGNDGPDFFTIRNFSPWSLSVAASTTDRRFLTGVQLGDGTNFTGVTINTFDL 360
            GILTS SAGN+GP  FT+ NFSPW+LSVAASTTDRRFLT VQLGDG  F GVTINTFDL
Sbjct: 301 NGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDL 360

Query: 361 NGTQYPLVYAGDIPNITAGFNASRSRFCLENSVDKELVRGKITLCDSILSPKELGSLKNA 420
           NGTQYPLV+AG+IPN+T GFN S SRFCL NSVD+ELV+GKI +CDSI+ P ++GSL++A
Sbjct: 361 NGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLESA 420

Query: 421 IGIIMQDRSPKDLTSSFPLPASHLGTQQ-GLISSYINLTSLPTATILKSTEGK--TSTFV 480
           +GIIMQD SPKDLT++FPLPASHLGTQQ  LISSY+NLT +PTATILKSTE K   +  V
Sbjct: 421 LGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPLV 480

Query: 481 ASFSSRGPNPITLDILKPDLSGPGVEILAAWSPIGSPSGAKDDNRNILYNIISGTSMSCP 540
           ASFSSRGPNP T DILKPD+ GPGVEILAAWSPI SPS AK DNR +L+NIISGTSM+CP
Sbjct: 481 ASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMACP 540

Query: 541 HATAVAAYVKSFHPSWSPAALKSALMTTAFPMKAELNSDAEFAYGSGHINPLSAINPGLI 600
           HATAVAAYVKSFHPSWSPAALKSAL+TTAFPM+ +L  +AEFAYGSGHINPL A+NPGLI
Sbjct: 541 HATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLI 600

Query: 601 YNATEIDYIRFLCGQGYSTKQLQQVSEDNNTCNSANNFDTVFNLNYPSFALSTTISTPIS 660
           YNA+EIDYIRFLCG+GY+T  L+ +++DN+TC S  N   V++LNY SFAL T ISTP S
Sbjct: 601 YNASEIDYIRFLCGEGYNTTLLRIITKDNSTC-SPTNSGLVYDLNYHSFALFTYISTPFS 660

Query: 661 QVYKRRVTNVGSANSTYKASIFAPLGLNIIVNPSVLSFKALGEEKNFEVTIEGSIDSGIA 720
           QVYKRRVTNVGS NSTYKA+IFAP  LNI VNPS LSFKAL EE  FEVTIEG I+  IA
Sbjct: 661 QVYKRRVTNVGSTNSTYKATIFAPSELNITVNPSTLSFKALEEELKFEVTIEGKINGSIA 720

Query: 721 SASLVWDDGLHKVRSPITVFDANTF 727
           SASLVWDDG+HKVRSPI VFD++TF
Sbjct: 721 SASLVWDDGVHKVRSPIIVFDSDTF 744

BLAST of Sed0004524 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 659.4 bits (1700), Expect = 3.2e-189
Identity = 354/705 (50.21%), Postives = 464/705 (65.82%), Query Frame = 0

Query: 26  MGSHSKDKVSTSSHHMRMLQETIGSTFSPHRLLHSYKRSFNGFVVKLSEAEAQKISEMEG 85
           MG+  + K S  SHH+ +LQ+ +G+  + H L+ SYKRSFNGF   LS+AE+QK+  M+ 
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 86  VISVFRNGRKELHTTRSWDFMGLSQQASRVPSIESDIIVGVFDSGIWPESPSFNDTGYSP 145
           V+SVF +   EL TTRSWDF+G  ++A R    ESD+IVGV DSGIWPES SF+D G+ P
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 146 PPPKWKGICEVSANFSCNNKIIGARSYRSDGLYSQTDLQSPRDSDGHGTHTASTVAGGLV 205
           PP KWKG C+    F+CNNK+IGAR Y            S RD +GHGTHTAST AG  V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYNKFA-------DSARDEEGHGTHTASTAAGNAV 180

Query: 206 RQASMLGLGLGTARGGVPSARIAAYKICWSDGCYDADILAAFDDAIADGVDIISFSVGGG 265
           + AS  GL  GTARGGVPSARIAAYK+C+ + C D DILAAFDDAIADGVD+IS S+   
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240

Query: 266 SPKYYFNDSIAIGAFHAMKKGILTSMSAGNDGPDFFTIRNFSPWSLSVAASTTDRRFLTG 325
                 N S+AIG+FHAM +GI+T+ SAGN+GPD  ++ N SPW ++VAAS TDR+F+  
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 326 VQLGDGTNFTGVTINTFDLNGTQYPLVYAGDIPNITAGFNASRSRFCLENSVDKELVRGK 385
           V LG+G   TG+++NTF+LNGT++P+VY     N++   + +++ +C    VD ELV+GK
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVYG---QNVSRNCSQAQAGYCSSGCVDSELVKGK 360

Query: 386 ITLCDSILSPKELGSLKNAIGIIMQDRSPKDLTSSFPLPASHLGTQQ-GLISSYINLTSL 445
           I LCD  L  +E   L  AIG+I+Q+    D     P PAS LG +    I SYI     
Sbjct: 361 IVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP 420

Query: 446 PTATILKSTE--GKTSTFVASFSSRGPNPITLDILKPDLSGPGVEILAAWSPIGSPSG-- 505
           P A IL++ E   + + +V SFSSRGP+ +  ++LKPD+S PG+EILAA+SP+ SPS   
Sbjct: 421 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 480

Query: 506 AKDDNRNILYNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAFPMKAELNSD 565
             +D R++ Y+++SGTSM+CPH   VAAYVKSFHP WSP+A+KSA+MTTA PM  + N +
Sbjct: 481 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 540

Query: 566 AEFAYGSGHINPLSAINPGLIYNATEIDYIRFLCGQGYSTKQLQQVSEDNNTCNSANNFD 625
            EFAYGSG INP  A +PGL+Y     DY++ LC +G+ +  L   S  N TC+      
Sbjct: 541 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERTE-- 600

Query: 626 TVFNLNYPSFALSTTISTPISQVYKRRVTNVGSANSTYKASIFAPL--GLNIIVNPSVLS 685
            V +LNYP+     +   P +  +KR VTNVG  NSTYKAS+  PL   L I + P +L 
Sbjct: 601 -VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASV-VPLQPELQISIEPEILR 660

Query: 686 FKALGEEKNFEVTIEGS--IDSGIASASLVWDDGLHKVRSPITVF 722
           F  L E+K+F VTI G    D    S+S+VW DG H VRSPI  +
Sbjct: 661 FGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAY 689

BLAST of Sed0004524 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 644.0 bits (1660), Expect = 1.4e-184
Identity = 354/738 (47.97%), Postives = 490/738 (66.40%), Query Frame = 0

Query: 1   MLSLPKLLFLSFCFS-SFQARTYIVYMGS-HSKDKVSTSSHHMRMLQETIGSTFSPHRLL 60
           +LS   +LFLS   + +   + YIVYMGS  S+   + +S HM +LQE  G +    RL+
Sbjct: 10  LLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSIEGRLV 69

Query: 61  HSYKRSFNGFVVKLSEAEAQKISEMEGVISVFRNGRKELHTTRSWDFMGLSQ--QASRVP 120
            SYKRSFNGF  +L+E+E +++++M GV+SVF N + +L TT SWDFMGL +  +  R P
Sbjct: 70  RSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNP 129

Query: 121 SIESDIIVGVFDSGIWPESPSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARSYRSDG 180
           ++ESD I+GV DSGI PES SF+D G+ PPP KWKG+C    NF+CNNK+IGAR Y S+G
Sbjct: 130 TVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTSEG 189

Query: 181 LYSQTDLQSPRDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYKICWSD 240
                     RD DGHGTHTAST AG  V  AS  G+G GT RGGVP++R+AAYK+C   
Sbjct: 190 ---------TRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPT 249

Query: 241 GCYDADILAAFDDAIADGVDIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSMSAGND 300
           GC    +L+AFDDAIADGVD+I+ S+G  +   + ND IAIGAFHAM KG+LT  SAGN 
Sbjct: 250 GCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNS 309

Query: 301 GPDFFTIRNFSPWSLSVAASTTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPLVYAGD 360
           GP   ++   +PW L+VAASTT+R F+T V LG+G    G ++N +++ G  YPLVY   
Sbjct: 310 GPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKS 369

Query: 361 IPNITAGFNASRSRFCLENSVDKELVRGKITLCDSILSPKELGSLKNAIGIIMQDRSPKD 420
               ++  +A  +  C  + VDK  V+GKI +C      K + S+  A+G+I +   P D
Sbjct: 370 A--ASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV-GAVGLIYRTPKP-D 429

Query: 421 LTSSFPLPASHLGTQQ-GLISSYINLTSLPTATILKSTE--GKTSTFVASFSSRGPNPIT 480
           +    PLPA+ L T+    + SY+  T  P A +LK+     +TS  +ASFSSRGPN I 
Sbjct: 430 VAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIA 489

Query: 481 LDILKPDLSGPGVEILAAWSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAAYVKSF 540
           +DILKPD++ PGVEILAA+SP G PS  +DD R++ Y+++SGTSMSCPH   VAAYVK+F
Sbjct: 490 VDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTF 549

Query: 541 HPSWSPAALKSALMTTAFPMKAELN--SDAEFAYGSGHINPLSAINPGLIYNATEIDYIR 600
           +P WSP+ ++SA+MTTA+P+ A     +  EFAYGSGH++P++A NPGL+Y   + D+I 
Sbjct: 550 NPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIA 609

Query: 601 FLCGQGYSTKQLQQVSEDNNTCNSANNFDTVFNLNYPSFALSTTIS-TPISQVYKRRVTN 660
           FLCG  Y+++ L+ +S +  TC+ A       NLNYPS +   + S T  +  + R +TN
Sbjct: 610 FLCGMNYTSQVLKVISGETVTCSEAKKI-LPRNLNYPSMSAKLSGSGTTFTVTFNRTLTN 669

Query: 661 VGSANSTYKASIFAPLG--LNIIVNPSVLSFKALGEEKNFEVTIEGS-IDSGI-ASASLV 720
           VG+ NSTY + + A  G  L++ + PSVLSFK + E+++F VT+ GS +DS + +SA+L+
Sbjct: 670 VGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLI 729

Query: 721 WDDGLHKVRSPITVFDAN 725
           W DG H VRSPI V+ ++
Sbjct: 730 WSDGTHNVRSPIVVYTSD 731

BLAST of Sed0004524 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 643.3 bits (1658), Expect = 2.4e-184
Identity = 355/718 (49.44%), Postives = 475/718 (66.16%), Query Frame = 0

Query: 20  RTYIVYMGSHSK--DKVSTSSHHMRMLQETIGSTFSPHRLLHSYKRSFNGFVVKLSEAEA 79
           + YIVYMGS S   D + TS  HM +LQ+  G +    RL+ SYKRSFNGF  +L+E+E 
Sbjct: 31  QVYIVYMGSLSSRADYIPTSD-HMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESER 90

Query: 80  QKISEMEGVISVFRNGRKELHTTRSWDFMGLSQ--QASRVPSIESDIIVGVFDSGIWPES 139
             I+E+EGV+SVF N   +LHTT SWDFMG+ +     R  +IESD I+GV D+GIWPES
Sbjct: 91  TLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPES 150

Query: 140 PSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARSYRSDGLYSQTDLQSPRDSDGHGTH 199
            SF+D G+ PPP KWKG+C    NF+CNNK+IGAR Y S+G          RD+ GHGTH
Sbjct: 151 KSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSEG---------TRDTSGHGTH 210

Query: 200 TASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYKICWSDGCYDADILAAFDDAIADGV 259
           TAST AG  V+  S  G+G GT RGGVP++RIAAYK+C   GC    +L++FDDAIADGV
Sbjct: 211 TASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGV 270

Query: 260 DIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSMSAGNDGPDFFTIRNFSPWSLSVAA 319
           D+I+ S+G   P  + +D IAIGAFHAM KGILT  SAGN GP   T+ + +PW  +VAA
Sbjct: 271 DLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAA 330

Query: 320 STTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPLVYAGDIPNITAGFNASRSRFCLEN 379
           STT+R F+T V LG+G    G ++N FD+ G +YPLVY       ++  +A  +  C   
Sbjct: 331 STTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSA--ASSACDAKTAALCAPA 390

Query: 380 SVDKELVRGKITLCDSILSPKELGSLKNAIGIIMQDRSPK-DLTSSFPLPASHLGTQQ-G 439
            ++K  V+GKI +C     P      K+   I + D+SP+ D+  +  LPAS L  +   
Sbjct: 391 CLNKSRVKGKILVCG---GPSGYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFK 450

Query: 440 LISSYINLTSLPTATILKSTE--GKTSTFVASFSSRGPNPITLDILKPDLSGPGVEILAA 499
            + SYI     P A +LK+     +TS  +ASFSSRGPN I +DILKPD++ PGVEILAA
Sbjct: 451 SLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAA 510

Query: 500 WSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAF 559
           +SP G PS  +DD R + Y++ SGTSM+CPH   VAAYVK+F+P WSP+ ++SA+MTTA+
Sbjct: 511 FSPNGEPS--EDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAW 570

Query: 560 PMKAELN--SDAEFAYGSGHINPLSAINPGLIYNATEIDYIRFLCGQGYSTKQLQQVSED 619
           P+KA+    +  EFAYG+GH++P++A+NPGL+Y   + D+I FLCG  Y++K L+ +S D
Sbjct: 571 PVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGD 630

Query: 620 NNTCNSANNFDTVFNLNYPSFA--LSTTISTPISQVYKRRVTNVGSANSTYKASIFAPLG 679
              C+  N      NLNYPS +  LS T ST  S  + R +TNVG+ NSTYK+ + A  G
Sbjct: 631 TVKCSKKNKI-LPRNLNYPSMSAKLSGTDST-FSVTFNRTLTNVGTPNSTYKSKVVAGHG 690

Query: 680 --LNIIVNPSVLSFKALGEEKNFEVTIEGS-IDSGI-ASASLVWDDGLHKVRSPITVF 722
             L+I V PSVL FK + E+++F VT+ GS +DS + +SA+L+W DG H VRSPI V+
Sbjct: 691 SKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVY 729

BLAST of Sed0004524 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 636.3 bits (1640), Expect = 2.9e-182
Identity = 353/716 (49.30%), Postives = 470/716 (65.64%), Query Frame = 0

Query: 20  RTYIVYMGSHSK--DKVSTSSHHMRMLQETIGSTFSPHRLLHSYKRSFNGFVVKLSEAEA 79
           + YIVYMGS S   D + TS  HM +LQ+  G +    RL+ SYKRSFNGF  +L+E+E 
Sbjct: 31  QVYIVYMGSLSSRADYIPTSD-HMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESER 90

Query: 80  QKISEMEGVISVFRNGRKELHTTRSWDFMGLSQ--QASRVPSIESDIIVGVFDSGIWPES 139
             I+E+EGV+SVF N   +LHTT SWDFMG+ +     R  +IESD I+GV D+GIWPES
Sbjct: 91  TLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPES 150

Query: 140 PSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARSYRSDGLYSQTDLQSPRDSDGHGTH 199
            SF+D G+ PPP KWKG+C    NF+CNNK+IGAR Y S+G          RD+ GHGTH
Sbjct: 151 KSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSEG---------TRDTSGHGTH 210

Query: 200 TASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYKICWSDGCYDADILAAFDDAIADGV 259
           TAST AG  V+  S  G+G GT RGGVP++RIAAYK+C   GC    +L++FDDAIADGV
Sbjct: 211 TASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGV 270

Query: 260 DIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSMSAGNDGPDFFTIRNFSPWSLSVAA 319
           D+I+ S+G   P  + +D IAIGAFHAM KGILT  SAGN GP   T+ + +PW  +VAA
Sbjct: 271 DLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAA 330

Query: 320 STTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPLVYAGDIPNITAGFNASRSRFCLEN 379
           STT+R F+T V LG+G    G ++N FD+ G +YPLVY       ++  +A  +  C   
Sbjct: 331 STTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSA--ASSACDAKTAALCAPA 390

Query: 380 SVDKELVRGKITLCDSILSPKELGSLKNAIGIIMQDRSPK-DLTSSFPLPASHLGTQQ-G 439
            ++K  V+GKI +C     P      K+   I + D+SP+ D+  +  LPAS L  +   
Sbjct: 391 CLNKSRVKGKILVCG---GPSGYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFK 450

Query: 440 LISSYINLTSLPTATILKSTE--GKTSTFVASFSSRGPNPITLDILKPDLSGPGVEILAA 499
            + SYI     P A +LK+     +TS  +ASFSSRGPN I +DILKPD++ PGVEILAA
Sbjct: 451 SLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAA 510

Query: 500 WSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAF 559
           +SP G PS  +DD R + Y++ SGTSM+CPH   VAAYVK+F+P WSP+ ++SA+MTTA 
Sbjct: 511 FSPNGEPS--EDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTA- 570

Query: 560 PMKAELNSDAEFAYGSGHINPLSAINPGLIYNATEIDYIRFLCGQGYSTKQLQQVSEDNN 619
             K    +  EFAYG+GH++P++A+NPGL+Y   + D+I FLCG  Y++K L+ +S D  
Sbjct: 571 --KGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTV 630

Query: 620 TCNSANNFDTVFNLNYPSFA--LSTTISTPISQVYKRRVTNVGSANSTYKASIFAPLG-- 679
            C+  N      NLNYPS +  LS T ST  S  + R +TNVG+ NSTYK+ + A  G  
Sbjct: 631 KCSKKNKI-LPRNLNYPSMSAKLSGTDST-FSVTFNRTLTNVGTPNSTYKSKVVAGHGSK 690

Query: 680 LNIIVNPSVLSFKALGEEKNFEVTIEGS-IDSGI-ASASLVWDDGLHKVRSPITVF 722
           L+I V PSVL FK + E+++F VT+ GS +DS + +SA+L+W DG H VRSPI V+
Sbjct: 691 LSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVY 724

BLAST of Sed0004524 vs. TAIR 10
Match: AT5G59090.3 (subtilase 4.12 )

HSP 1 Score: 636.0 bits (1639), Expect = 3.8e-182
Identity = 354/718 (49.30%), Postives = 473/718 (65.88%), Query Frame = 0

Query: 20  RTYIVYMGSHSK--DKVSTSSHHMRMLQETIGSTFSPHRLLHSYKRSFNGFVVKLSEAEA 79
           + YIVYMGS S   D + TS  HM +LQ+  G +    RL+ SYKRSFNGF  +L+E+E 
Sbjct: 31  QVYIVYMGSLSSRADYIPTSD-HMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESER 90

Query: 80  QKISEMEGVISVFRNGRKELHTTRSWDFMGLSQ--QASRVPSIESDIIVGVFDSGIWPES 139
             I+  EGV+SVF N   +LHTT SWDFMG+ +     R  +IESD I+GV D+GIWPES
Sbjct: 91  TLIA--EGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPES 150

Query: 140 PSFNDTGYSPPPPKWKGICEVSANFSCNNKIIGARSYRSDGLYSQTDLQSPRDSDGHGTH 199
            SF+D G+ PPP KWKG+C    NF+CNNK+IGAR Y S+G          RD+ GHGTH
Sbjct: 151 KSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSEG---------TRDTSGHGTH 210

Query: 200 TASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYKICWSDGCYDADILAAFDDAIADGV 259
           TAST AG  V+  S  G+G GT RGGVP++RIAAYK+C   GC    +L++FDDAIADGV
Sbjct: 211 TASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGV 270

Query: 260 DIISFSVGGGSPKYYFNDSIAIGAFHAMKKGILTSMSAGNDGPDFFTIRNFSPWSLSVAA 319
           D+I+ S+G   P  + +D IAIGAFHAM KGILT  SAGN GP   T+ + +PW  +VAA
Sbjct: 271 DLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAA 330

Query: 320 STTDRRFLTGVQLGDGTNFTGVTINTFDLNGTQYPLVYAGDIPNITAGFNASRSRFCLEN 379
           STT+R F+T V LG+G    G ++N FD+ G +YPLVY       ++  +A  +  C   
Sbjct: 331 STTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSA--ASSACDAKTAALCAPA 390

Query: 380 SVDKELVRGKITLCDSILSPKELGSLKNAIGIIMQDRSPK-DLTSSFPLPASHLGTQQ-G 439
            ++K  V+GKI +C     P      K+   I + D+SP+ D+  +  LPAS L  +   
Sbjct: 391 CLNKSRVKGKILVCG---GPSGYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFK 450

Query: 440 LISSYINLTSLPTATILKSTE--GKTSTFVASFSSRGPNPITLDILKPDLSGPGVEILAA 499
            + SYI     P A +LK+     +TS  +ASFSSRGPN I +DILKPD++ PGVEILAA
Sbjct: 451 SLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAA 510

Query: 500 WSPIGSPSGAKDDNRNILYNIISGTSMSCPHATAVAAYVKSFHPSWSPAALKSALMTTAF 559
           +SP G PS  +DD R + Y++ SGTSM+CPH   VAAYVK+F+P WSP+ ++SA+MTTA+
Sbjct: 511 FSPNGEPS--EDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAW 570

Query: 560 PMKAELN--SDAEFAYGSGHINPLSAINPGLIYNATEIDYIRFLCGQGYSTKQLQQVSED 619
           P+KA+    +  EFAYG+GH++P++A+NPGL+Y   + D+I FLCG  Y++K L+ +S D
Sbjct: 571 PVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGD 630

Query: 620 NNTCNSANNFDTVFNLNYPSFA--LSTTISTPISQVYKRRVTNVGSANSTYKASIFAPLG 679
              C+  N      NLNYPS +  LS T ST  S  + R +TNVG+ NSTYK+ + A  G
Sbjct: 631 TVKCSKKNKI-LPRNLNYPSMSAKLSGTDST-FSVTFNRTLTNVGTPNSTYKSKVVAGHG 690

Query: 680 --LNIIVNPSVLSFKALGEEKNFEVTIEGS-IDSGI-ASASLVWDDGLHKVRSPITVF 722
             L+I V PSVL FK + E+++F VT+ GS +DS + +SA+L+W DG H VRSPI V+
Sbjct: 691 SKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVY 727