Sed0004329 (gene) Chayote v1

BLAST of Sed0004329 vs. NCBI nr
Match: XP_022149204.1 (serine hydroxymethyltransferase 7-like [Momordica charantia] >XP_022149205.1 serine hydroxymethyltransferase 7-like [Momordica charantia] >XP_022149207.1 serine hydroxymethyltransferase 7-like [Momordica charantia])

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 533/588 (90.65%), Postives = 556/588 (94.56%), Query Frame = 0

Query: 6   MDLSNPHSNLSLGFSSHASPPHRPPIADDSIPIHLDSSRRESSSHSSPPVPLQLMEPQTE 65
           MDLSN HSNLSLGFSSHASPPHR  IADDS+ +HLDS+RRE   +SSPPVPLQLMEPQTE
Sbjct: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRRE-PPYSSPPVPLQLMEPQTE 60

Query: 66  SLRDDDGDA--DGDEYDDREVEEFRILGHSMCLKRRRDGQASSTLSSAKRISCEFDFETR 125
           S RD+DG+A  + D+YDDREVEEFRILGHSMCLKRRRDG++SS+  S KR+SCEFD + R
Sbjct: 61  SHRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDAR 120

Query: 126 RSVVRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHLTNKYS 185
           RS VRSWGNQ LRVADPNLFGIMEKEK RQFKGIELIASENYVCRAVMEALGSHLTNKYS
Sbjct: 121 RSAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYS 180

Query: 186 EGMPGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYTGLLLP 245
           EGMPGARYYGGN+YIDEIETLC ERALAAFGLDSD+WGVNVQPYSCTSANFAVYTGLLLP
Sbjct: 181 EGMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLP 240

Query: 246 GSRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKERALDF 305
           G RIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKL+ERALDF
Sbjct: 241 GDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDF 300

Query: 306 RPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 365
           RPKILICGGSSYPRE DYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS
Sbjct: 301 RPKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 360

Query: 366 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGGPHNNH 425
           TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQG+ETDQYDFEEKINFAVFPSLQGGPHNNH
Sbjct: 361 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNH 420

Query: 426 IAALAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDLRSLGL 485
           IAALAVALKQ+ATPEYKAYMQQVKKNAQAL +ALLRKKYRLVTGGTDNHMLLWDLR LGL
Sbjct: 421 IAALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGL 480

Query: 486 TGKNYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIADFLHAA 545
           TGKNYEKVCEIC ITLNKITI+GDNG IIPGGVRIGTPAMTSRGCLESDFETI DFLH A
Sbjct: 481 TGKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTA 540

Query: 546 AQIAISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPGIE 592
           AQIAISVQRE GKLPK YLKGLQ+N EVVEL+NRVESFSAQFA+PG E
Sbjct: 541 AQIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587

BLAST of Sed0004329 vs. NCBI nr
Match: XP_022941930.1 (serine hydroxymethyltransferase 7-like [Cucurbita moschata] >KAG6600365.1 Serine hydroxymethyltransferase 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031023.1 Serine hydroxymethyltransferase 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 537/588 (91.33%), Postives = 556/588 (94.56%), Query Frame = 0

Query: 6   MDLSNPHSNLSLGFSSHASPPHRPPIADDSIPIHLDSSRRESSSHSSPPVPLQLMEPQTE 65
           MDLS  HSNLSLGFSSHASPPHR  IADDS+ +HLDSSRRE SS+SS PVPLQLME QTE
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRE-SSYSSTPVPLQLMEQQTE 60

Query: 66  SLRDDDGDA--DGDEYDDREVEEFRILGHSMCLKRRRDGQASSTLSSAKRISCEFDFETR 125
           S R+D GDA  D DEYDDREVEEFRILGHSMCLKRRRDG++S++  SAKRISCEFD ETR
Sbjct: 61  SHRNDGGDAERDDDEYDDREVEEFRILGHSMCLKRRRDGESSTSSGSAKRISCEFDIETR 120

Query: 126 RSVVRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHLTNKYS 185
           RS VRSWG+Q LRVADPNLFGIMEKEK+RQFKGIELIASENYVCRAVMEALGSHLTNKYS
Sbjct: 121 RSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYS 180

Query: 186 EGMPGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYTGLLLP 245
           EGMPGARYY GN+YIDEIETLC ERALAAFGL SD+WGVNVQPYSCTSANFAVYTGLLLP
Sbjct: 181 EGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLP 240

Query: 246 GSRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKERALDF 305
           G RIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKL+ERALDF
Sbjct: 241 GDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDF 300

Query: 306 RPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 365
           RPKILICGGSSYPRE DYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS
Sbjct: 301 RPKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 360

Query: 366 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGGPHNNH 425
           TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQG+ETDQYDFEEKINFAVFPSLQGGPHNNH
Sbjct: 361 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNH 420

Query: 426 IAALAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDLRSLGL 485
           IAALAVALKQVATPEYKAYMQQVKKNAQAL AALLRKKY+LVTGGTDNHM+LWDLRSLGL
Sbjct: 421 IAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGL 480

Query: 486 TGKNYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIADFLHAA 545
           TGKNYE VCEICHITLNKITIFGDNG IIPGGVR+GTPAMTSRGCLESDFETIADFLH A
Sbjct: 481 TGKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTA 540

Query: 546 AQIAISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPGIE 592
           AQIAISVQRE GKLPK YLKGL SN EVVEL+NRVESFSAQFA+PG E
Sbjct: 541 AQIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

BLAST of Sed0004329 vs. NCBI nr
Match: XP_022979958.1 (serine hydroxymethyltransferase 7-like [Cucurbita maxima])

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 536/588 (91.16%), Postives = 555/588 (94.39%), Query Frame = 0

Query: 6   MDLSNPHSNLSLGFSSHASPPHRPPIADDSIPIHLDSSRRESSSHSSPPVPLQLMEPQTE 65
           MDLS  HSNLSLGFSSHASPPHR  IADDS+ +HLDSSRRE SS+SS PVPLQLME QTE
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRE-SSYSSTPVPLQLMEQQTE 60

Query: 66  SLRDDDGDA--DGDEYDDREVEEFRILGHSMCLKRRRDGQASSTLSSAKRISCEFDFETR 125
           S R+D GDA  D DEYDDREVEEFRILGH MCLKRRRDG++S++ SSAKRISCEFD ETR
Sbjct: 61  SHRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETR 120

Query: 126 RSVVRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHLTNKYS 185
           RS VRSWG+Q LRVADPNLFGIMEKEK+RQFKGIELIASENYVCRAVMEALGSHLTNKYS
Sbjct: 121 RSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYS 180

Query: 186 EGMPGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYTGLLLP 245
           EGMPGARYY GN+YIDEIETLC ERALAAFGL SD+WGVNVQPYSCTSANFAVYTGLLLP
Sbjct: 181 EGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLP 240

Query: 246 GSRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKERALDF 305
           G RIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKL+ERALDF
Sbjct: 241 GDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDF 300

Query: 306 RPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 365
           RPKILICGGSSYPRE DYARFRQIADKCGA LMCDMAQISGLVAAKECVSPFGYCDVVTS
Sbjct: 301 RPKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTS 360

Query: 366 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGGPHNNH 425
           TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQG+ETDQYDFEEKINFAVFPSLQGGPHNNH
Sbjct: 361 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNH 420

Query: 426 IAALAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDLRSLGL 485
           IAALAVALKQVATPEYKAYMQQVKKNAQAL AALLRKKY+LVTGGTDNHM+LWDLRSLGL
Sbjct: 421 IAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGL 480

Query: 486 TGKNYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIADFLHAA 545
           TGKNYE VCEICHITLNKITIFGDNG IIPGGVR+GTPAMTSRGCLESDFETIADFLH A
Sbjct: 481 TGKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTA 540

Query: 546 AQIAISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPGIE 592
           AQIAISVQRE GKLPK YLKGL SN EVVEL+NRVESFSAQFA+PG E
Sbjct: 541 AQIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

BLAST of Sed0004329 vs. NCBI nr
Match: XP_023547556.1 (serine hydroxymethyltransferase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 535/588 (90.99%), Postives = 555/588 (94.39%), Query Frame = 0

Query: 6   MDLSNPHSNLSLGFSSHASPPHRPPIADDSIPIHLDSSRRESSSHSSPPVPLQLMEPQTE 65
           MDLS  HSNLSLGFSSHASPPHR  IADDS+ +HLDSSRRE SS+SS PVPLQLME QTE
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRE-SSYSSTPVPLQLMEQQTE 60

Query: 66  SLRDD--DGDADGDEYDDREVEEFRILGHSMCLKRRRDGQASSTLSSAKRISCEFDFETR 125
           S R+D  DG+ D DEYDDREVEEFRILGH MCLKRRRDG++S++  SAKRISCEFD ETR
Sbjct: 61  SHRNDGGDGERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSGSAKRISCEFDIETR 120

Query: 126 RSVVRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHLTNKYS 185
           RS VRSWG+Q LRVADPNLFGIMEKEK+RQFKGIELIASENYVCRAVMEALGSHLTNKYS
Sbjct: 121 RSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYS 180

Query: 186 EGMPGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYTGLLLP 245
           EGMPGARYY GN+YIDEIETLC ERALAAFGL SD+WGVNVQPYSCTSANFAVYTGLLLP
Sbjct: 181 EGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLP 240

Query: 246 GSRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKERALDF 305
           G RIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKL+ERALDF
Sbjct: 241 GDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDF 300

Query: 306 RPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 365
           RPKILICGGSSYPRE DYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS
Sbjct: 301 RPKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 360

Query: 366 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGGPHNNH 425
           TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQG+ETDQYDFEEKINFAVFPSLQGGPHNNH
Sbjct: 361 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNH 420

Query: 426 IAALAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDLRSLGL 485
           IAALAVALKQVATPEYKAYMQQVKKNAQAL AALLRKKY+LVTGGTDNHM+LWDLRSLGL
Sbjct: 421 IAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGL 480

Query: 486 TGKNYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIADFLHAA 545
           TGKNYE VCEICHITLNKITIFGDNG IIPGGVR+GTPAMTSRGCLESDFETIADFLH A
Sbjct: 481 TGKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTA 540

Query: 546 AQIAISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPGIE 592
           AQIAISVQRE GKLPK YLKGL SN EVVEL+NRVESFSAQFA+PG E
Sbjct: 541 AQIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

BLAST of Sed0004329 vs. NCBI nr
Match: KAG6576983.1 (Serine hydroxymethyltransferase 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015003.1 Serine hydroxymethyltransferase 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1032.3 bits (2668), Expect = 1.6e-297
Identity = 511/589 (86.76%), Postives = 540/589 (91.68%), Query Frame = 0

Query: 6   MDLSNPHSNLSLGFSSHASPPHRPPIADDSIPIHLDSSRRESSSHSSPPVPLQLMEPQTE 65
           MDLSN HSNLSLG SSHASPPHR  IADDSI +HL+SSR+E  + SSPP+PLQLMEP TE
Sbjct: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQE-PTFSSPPIPLQLMEPLTE 60

Query: 66  SLRDDDGDA--DGDEYDDREVEEFRILGHSMCLKRRRDGQASSTLSSAKRISCEFDFETR 125
           S RD+ GD   + D YDDR+VEEFRILGHSMCLKRRRD ++S + SSAKR SCE D ETR
Sbjct: 61  SHRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETR 120

Query: 126 RSVVRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHLTNKYS 185
           RS VRSWGNQ LR ADPNLFGIMEKEK+RQ KGIELIASENYVCRAVMEALGSHLTNKYS
Sbjct: 121 RSAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYS 180

Query: 186 EGMPGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYTGLLLP 245
           EGMPGARYY GN+YIDEIETLC ERALAAFGLDSD WGVNVQPYSCTSANFAVYTGLLLP
Sbjct: 181 EGMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLP 240

Query: 246 GSRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKERALDF 305
           G RIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKL+ERALDF
Sbjct: 241 GDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDF 300

Query: 306 RPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 365
           RPKILICGGSSYPRE DY RFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS
Sbjct: 301 RPKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 360

Query: 366 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGGPHNNH 425
           TTHKSLRGPRGGIIFYRKGSKPR+RAMLLYQG+E +QYDFEEKINFAVFPSLQGGPHNNH
Sbjct: 361 TTHKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNH 420

Query: 426 IAALAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDLRSLGL 485
           IAALAVALKQVAT EYKAYMQQVKKNAQAL +ALLRK Y+LVTGGTDNHM+LWDLRSLGL
Sbjct: 421 IAALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGL 480

Query: 486 TGKNYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIADFLHAA 545
           TGKN+E+VCE+CHITLNKITIFGDNG I PGGVRIGTPAMTSRGCLESDFE I +FLH A
Sbjct: 481 TGKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIA 540

Query: 546 AQIAISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPGIET 593
           AQIAI VQRE GKLPK +L+GLQSN EVVEL  RV SFSA+FA+PG+ET
Sbjct: 541 AQIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET 588

BLAST of Sed0004329 vs. ExPASy Swiss-Prot
Match: Q84WV0 (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 1.1e-237
Identity = 413/592 (69.76%), Postives = 477/592 (80.57%), Query Frame = 0

Query: 6   MDLSNPHSNLSLGFS-SHAS----PPHRPPIADDSIPIHLDSSRRESSSHSSPPVPLQLM 65
           MDLS   +N  LGF  SHAS    P  R PIADDSI + +D S R   +  S P+PLQL+
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFS-PIPLQLL 60

Query: 66  EPQTESLR--DDDGDADGDEYDDREVEEFRILGHSMCLKRRRDGQASSTLSS-AKRISC- 125
           E + E     D+     G   D +E E FRILGH MCLKR+RD     T S   KR S  
Sbjct: 61  EQKAEKTTTVDEPKKDGGGGGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSIG 120

Query: 126 EFDFETRRSVVRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGS 185
           + D E+RR+ VR+WG+Q + +ADP++  +MEKEK+RQ +GIELIASEN+VCRAVMEALGS
Sbjct: 121 DSDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGS 180

Query: 186 HLTNKYSEGMPGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAV 245
           HLTNKYSEGMPGARYY GN+YID+IE LC ERAL AFGL+SD WGVNVQPYSCTSANFAV
Sbjct: 181 HLTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAV 240

Query: 246 YTGLLLPGSRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKL 305
           YTGLLLPG RIMGLD+PSGG+ SHGYCTP G+K+S ASIFFES PYKVNPQTGYIDYDKL
Sbjct: 241 YTGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKL 300

Query: 306 KERALDFRPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFG 365
           +++ALD+RPKILICGGSSYPR+WD+AR RQIADKCGAVLMCDMA ISGLVA KEC +PF 
Sbjct: 301 EDKALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFD 360

Query: 366 YCDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQ 425
           +CD+VTSTTHK LRGPRGGIIFYR+G K RK+         +  YD EEKINFAVFPSLQ
Sbjct: 361 HCDIVTSTTHKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQ 420

Query: 426 GGPHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLW 485
           GGPHNNHIAALA+ALKQVATPEYKAY+QQ+KKNAQAL AALLR+K RLVTGGTDNH+LLW
Sbjct: 421 GGPHNNHIAALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLW 480

Query: 486 DLRSLGLTGKNYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETI 545
           DL  +GLTGK YEKVCE+CHITLNK  IFGDNGTI PGGVRIGTPAMT+RGC+ESDFET+
Sbjct: 481 DLTPMGLTGKVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETM 540

Query: 546 ADFLHAAAQIAISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALP 589
           ADFL  AAQI  ++QRE GK  K ++K L +N ++ EL+NRVE+F+ Q+ +P
Sbjct: 541 ADFLIKAAQITSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

BLAST of Sed0004329 vs. ExPASy Swiss-Prot
Match: Q9LM59 (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 SV=1)

HSP 1 Score: 813.1 bits (2099), Expect = 2.0e-234
Identity = 406/593 (68.47%), Postives = 482/593 (81.28%), Query Frame = 0

Query: 13  SNLSLGF-SSHASP-PHRP--PIADDSIPIHLDSSRRESSSHSSPPVPLQLMEPQTESL- 72
           S+LSLGF SSHA P PH P  PIADDSI + +DSS R  SS+  PPVPLQL+E + +   
Sbjct: 7   SDLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFR-PSSNPMPPVPLQLLEQRFDVTG 66

Query: 73  -------RDDDGDADGDEYDDREVEEFRILGHSMCLKRRRDGQASSTLSSA--KRISCEF 132
                   DD+   D D+ D RE E+F +LGH M LKR R G + S  SS+  KR   + 
Sbjct: 67  SCSRVVEEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVVDS 126

Query: 133 DFETRRSVVRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHL 192
             E+RR+VVR+WGNQ +  ADP +   MEKEK+RQF+GIELIASEN+VCRAVMEALGSHL
Sbjct: 127 GIESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHL 186

Query: 193 TNKYSEGMPGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYT 252
           TNKYSEGMPGARYY GN+YID+IE LC ERALAAFGL+ + WGVNVQPYSCTSANFAV+T
Sbjct: 187 TNKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFT 246

Query: 253 GLLLPGSRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKE 312
           GLL+PG RIMGLD+PSGG+ SHGY TP G+KVSGASIFFES PYKV+P+TGYIDYDKL+E
Sbjct: 247 GLLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEE 306

Query: 313 RALDFRPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYC 372
           +ALD+RPKILICGGSSYPR+W++ RFR IADKCGAVLM DMAQISGLVAAKE  +PF YC
Sbjct: 307 KALDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYC 366

Query: 373 DVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGG 432
           D+VTSTTHKSLRGPRGGIIFY++G KP+K+++ L       QYDFEEKINF+VFPSLQGG
Sbjct: 367 DIVTSTTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGG 426

Query: 433 PHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDL 492
           PHNNHIAALA+ALKQ A+PEYK YM+QVKKNA+AL +AL+ +K +L+TGGTDNH+LLWDL
Sbjct: 427 PHNNHIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDL 486

Query: 493 RSLGLTGKNYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIAD 552
             LGLTGK YEKVCE+CHIT+NK+ IF +NG I PGGVRIG+PAMTSRGCLE +FET+AD
Sbjct: 487 TPLGLTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMAD 546

Query: 553 FLHAAAQIAISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPGIE 592
           FL+ AAQIA + QRE GKL K  LK +    E+ +L+N+VE+F+ QFA+P  +
Sbjct: 547 FLYRAAQIASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFD 598

BLAST of Sed0004329 vs. ExPASy Swiss-Prot
Match: O23254 (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 1.8e-174
Identity = 296/463 (63.93%), Postives = 353/463 (76.24%), Query Frame = 0

Query: 127 VRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 186
           V SWGN  L   DP +  ++EKEKRRQ +GIELIASEN+   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 187 PGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYTGLLLPGSR 246
           PG RYYGGN +IDEIE LC  RAL AF  D   WGVNVQPYS + ANFA YT LL P  R
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 247 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKERALDFRPK 306
           IMGLD PSGG+ +HGY T  G+K+S  SI+FESLPYKVN  TGYIDYDKL+E+ALDFRPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 307 ILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 366
           +LICGGS+YPR+WDYARFR IADK GA+L+CDMA ISGLVAA+E  +PF YCDVVT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 367 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGGPHNNHIAA 426
           KSLRGPR G+IFYRKG KP K+        E   YDFE+KINFAVFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 427 LAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDLRSLGLTGK 486
           LAVALKQ  TP +K Y +QVK NA AL   L+ K Y++VT GT+NH++LWDLR LGLTG 
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 487 NYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIADFLHAAAQI 546
             EK+C++C ITLNK  +FGD+  + PGGVRIG PAMTSRG +E DFE I +FL  A  +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 547 AISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPG 590
            + +Q+  GKL K + KGL +N ++ +L+  VE FSA + +PG
Sbjct: 424 TLDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPG 461

BLAST of Sed0004329 vs. ExPASy Swiss-Prot
Match: Q9SVM4 (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 8.3e-172
Identity = 286/463 (61.77%), Postives = 348/463 (75.16%), Query Frame = 0

Query: 127 VRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 186
           V SWGN  L   DP ++ ++EKEK RQ +GIELIA+EN+   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 187 PGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYTGLLLPGSR 246
           PG RYYGG  +IDEIE+LC  R+L AF  + + WGVNVQPYS + ANFA YT LL P  R
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 247 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKERALDFRPK 306
           IMGLD PSGG+ +HGY +  G+ +S  SI+FE+LPYKV+ +TGYIDYDKL+E+A+DFRPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 307 ILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 366
           ++ICGG+SYPREWDYARFR +ADK GA L+CDMA  S LVAA+E   PF YCDVVT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 367 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGGPHNNHIAA 426
           KSLRGPR G+IFYRKG KP K+        E + YDF+ KIN AVFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKGQ-----PEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 427 LAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDLRSLGLTGK 486
           LAVALKQV  P +K Y +QVK NA  L + L+ K Y LVT GTDNH++LWDLR LGLTG 
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 487 NYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIADFLHAAAQI 546
             EKVCE+C+ITLN+  +FGD   + PGGVRIGTPAMTSRG +E DFE I +FLH A  I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 547 AISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPG 590
            + +Q + GK+ K + KGL +N E+ E++  VE F+  F +PG
Sbjct: 424 TLDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPG 461

BLAST of Sed0004329 vs. ExPASy Swiss-Prot
Match: Q54Z26 (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 PE=1 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.7e-154
Identity = 268/460 (58.26%), Postives = 334/460 (72.61%), Query Frame = 0

Query: 131 GNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGMPGAR 190
           GN  L+  D  +F +M +EK RQFKG+ELIASEN+  RAVMEALGSH TNKY+EG PG+R
Sbjct: 6   GNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEALGSHFTNKYAEGYPGSR 65

Query: 191 YYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYTGLLLPGSRIMGL 250
           YYGG   +DE+ETLC +RAL AF LD   WGVNVQPYS + ANFAVYT LL P  RIMGL
Sbjct: 66  YYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPANFAVYTALLRPHDRIMGL 125

Query: 251 DTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKERALDFRPKILIC 310
           D PSGG+ +HGY T + +K+S +SIFFES+PY++    G IDY +L+E AL F+PK++I 
Sbjct: 126 DLPSGGHLTHGYQT-DKKKISASSIFFESMPYQIGAD-GLIDYQRLEENALLFKPKLIIS 185

Query: 311 GGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTHKSLR 370
           G S+YPREWDY R R IADK GA LMCDMA  SGLVAA+   SPF YCDVVTSTTHK+LR
Sbjct: 186 GASAYPREWDYKRMRAIADKVGAYLMCDMAHYSGLVAAQLLDSPFDYCDVVTSTTHKTLR 245

Query: 371 GPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGGPHNNHIAALAVA 430
           GPR GIIF+R+G +      +   G E ++YD E KINFAVFPSLQGGPH N IA +AVA
Sbjct: 246 GPRSGIIFFRRGKR------VDGNGKEIEEYDIESKINFAVFPSLQGGPHENVIAGVAVA 305

Query: 431 LKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDLRSLGLTGKNYEK 490
           LK+  + E+K Y  QVKKNA A+  AL+ K Y+LVT GTDNH++LWDLR   LTG  +EK
Sbjct: 306 LKEADSQEFKEYALQVKKNAAAIGNALMNKGYKLVTNGTDNHLILWDLRPKELTGNKFEK 365

Query: 491 VCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIADFLHAAAQIAISV 550
             +I +IT+NK  + GD   I PGG+RIG+ A+TSRG  E+DFE IADFL     I++ +
Sbjct: 366 AADIANITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSISLEI 425

Query: 551 QRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPGI 591
           Q   GK    ++  +  + E+++L+  VE FS++F LPGI
Sbjct: 426 QGRVGKKLVDFVVEINKSKELLDLRKEVEEFSSKFTLPGI 457

BLAST of Sed0004329 vs. ExPASy TrEMBL
Match: A0A6J1D7A8 (Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111017683 PE=3 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 533/588 (90.65%), Postives = 556/588 (94.56%), Query Frame = 0

Query: 6   MDLSNPHSNLSLGFSSHASPPHRPPIADDSIPIHLDSSRRESSSHSSPPVPLQLMEPQTE 65
           MDLSN HSNLSLGFSSHASPPHR  IADDS+ +HLDS+RRE   +SSPPVPLQLMEPQTE
Sbjct: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRRE-PPYSSPPVPLQLMEPQTE 60

Query: 66  SLRDDDGDA--DGDEYDDREVEEFRILGHSMCLKRRRDGQASSTLSSAKRISCEFDFETR 125
           S RD+DG+A  + D+YDDREVEEFRILGHSMCLKRRRDG++SS+  S KR+SCEFD + R
Sbjct: 61  SHRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDAR 120

Query: 126 RSVVRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHLTNKYS 185
           RS VRSWGNQ LRVADPNLFGIMEKEK RQFKGIELIASENYVCRAVMEALGSHLTNKYS
Sbjct: 121 RSAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYS 180

Query: 186 EGMPGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYTGLLLP 245
           EGMPGARYYGGN+YIDEIETLC ERALAAFGLDSD+WGVNVQPYSCTSANFAVYTGLLLP
Sbjct: 181 EGMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLP 240

Query: 246 GSRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKERALDF 305
           G RIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKL+ERALDF
Sbjct: 241 GDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDF 300

Query: 306 RPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 365
           RPKILICGGSSYPRE DYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS
Sbjct: 301 RPKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 360

Query: 366 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGGPHNNH 425
           TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQG+ETDQYDFEEKINFAVFPSLQGGPHNNH
Sbjct: 361 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNH 420

Query: 426 IAALAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDLRSLGL 485
           IAALAVALKQ+ATPEYKAYMQQVKKNAQAL +ALLRKKYRLVTGGTDNHMLLWDLR LGL
Sbjct: 421 IAALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGL 480

Query: 486 TGKNYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIADFLHAA 545
           TGKNYEKVCEIC ITLNKITI+GDNG IIPGGVRIGTPAMTSRGCLESDFETI DFLH A
Sbjct: 481 TGKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTA 540

Query: 546 AQIAISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPGIE 592
           AQIAISVQRE GKLPK YLKGLQ+N EVVEL+NRVESFSAQFA+PG E
Sbjct: 541 AQIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587

BLAST of Sed0004329 vs. ExPASy TrEMBL
Match: A0A6J1FV71 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447145 PE=3 SV=1)

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 537/588 (91.33%), Postives = 556/588 (94.56%), Query Frame = 0

Query: 6   MDLSNPHSNLSLGFSSHASPPHRPPIADDSIPIHLDSSRRESSSHSSPPVPLQLMEPQTE 65
           MDLS  HSNLSLGFSSHASPPHR  IADDS+ +HLDSSRRE SS+SS PVPLQLME QTE
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRE-SSYSSTPVPLQLMEQQTE 60

Query: 66  SLRDDDGDA--DGDEYDDREVEEFRILGHSMCLKRRRDGQASSTLSSAKRISCEFDFETR 125
           S R+D GDA  D DEYDDREVEEFRILGHSMCLKRRRDG++S++  SAKRISCEFD ETR
Sbjct: 61  SHRNDGGDAERDDDEYDDREVEEFRILGHSMCLKRRRDGESSTSSGSAKRISCEFDIETR 120

Query: 126 RSVVRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHLTNKYS 185
           RS VRSWG+Q LRVADPNLFGIMEKEK+RQFKGIELIASENYVCRAVMEALGSHLTNKYS
Sbjct: 121 RSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYS 180

Query: 186 EGMPGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYTGLLLP 245
           EGMPGARYY GN+YIDEIETLC ERALAAFGL SD+WGVNVQPYSCTSANFAVYTGLLLP
Sbjct: 181 EGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLP 240

Query: 246 GSRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKERALDF 305
           G RIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKL+ERALDF
Sbjct: 241 GDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDF 300

Query: 306 RPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 365
           RPKILICGGSSYPRE DYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS
Sbjct: 301 RPKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 360

Query: 366 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGGPHNNH 425
           TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQG+ETDQYDFEEKINFAVFPSLQGGPHNNH
Sbjct: 361 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNH 420

Query: 426 IAALAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDLRSLGL 485
           IAALAVALKQVATPEYKAYMQQVKKNAQAL AALLRKKY+LVTGGTDNHM+LWDLRSLGL
Sbjct: 421 IAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGL 480

Query: 486 TGKNYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIADFLHAA 545
           TGKNYE VCEICHITLNKITIFGDNG IIPGGVR+GTPAMTSRGCLESDFETIADFLH A
Sbjct: 481 TGKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTA 540

Query: 546 AQIAISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPGIE 592
           AQIAISVQRE GKLPK YLKGL SN EVVEL+NRVESFSAQFA+PG E
Sbjct: 541 AQIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

BLAST of Sed0004329 vs. ExPASy TrEMBL
Match: A0A6J1IXT5 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111479489 PE=3 SV=1)

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 536/588 (91.16%), Postives = 555/588 (94.39%), Query Frame = 0

Query: 6   MDLSNPHSNLSLGFSSHASPPHRPPIADDSIPIHLDSSRRESSSHSSPPVPLQLMEPQTE 65
           MDLS  HSNLSLGFSSHASPPHR  IADDS+ +HLDSSRRE SS+SS PVPLQLME QTE
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRE-SSYSSTPVPLQLMEQQTE 60

Query: 66  SLRDDDGDA--DGDEYDDREVEEFRILGHSMCLKRRRDGQASSTLSSAKRISCEFDFETR 125
           S R+D GDA  D DEYDDREVEEFRILGH MCLKRRRDG++S++ SSAKRISCEFD ETR
Sbjct: 61  SHRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETR 120

Query: 126 RSVVRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHLTNKYS 185
           RS VRSWG+Q LRVADPNLFGIMEKEK+RQFKGIELIASENYVCRAVMEALGSHLTNKYS
Sbjct: 121 RSAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYS 180

Query: 186 EGMPGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYTGLLLP 245
           EGMPGARYY GN+YIDEIETLC ERALAAFGL SD+WGVNVQPYSCTSANFAVYTGLLLP
Sbjct: 181 EGMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLP 240

Query: 246 GSRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKERALDF 305
           G RIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKL+ERALDF
Sbjct: 241 GDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDF 300

Query: 306 RPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 365
           RPKILICGGSSYPRE DYARFRQIADKCGA LMCDMAQISGLVAAKECVSPFGYCDVVTS
Sbjct: 301 RPKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTS 360

Query: 366 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGGPHNNH 425
           TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQG+ETDQYDFEEKINFAVFPSLQGGPHNNH
Sbjct: 361 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNH 420

Query: 426 IAALAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDLRSLGL 485
           IAALAVALKQVATPEYKAYMQQVKKNAQAL AALLRKKY+LVTGGTDNHM+LWDLRSLGL
Sbjct: 421 IAALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGL 480

Query: 486 TGKNYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIADFLHAA 545
           TGKNYE VCEICHITLNKITIFGDNG IIPGGVR+GTPAMTSRGCLESDFETIADFLH A
Sbjct: 481 TGKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTA 540

Query: 546 AQIAISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPGIE 592
           AQIAISVQRE GKLPK YLKGL SN EVVEL+NRVESFSAQFA+PG E
Sbjct: 541 AQIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

BLAST of Sed0004329 vs. ExPASy TrEMBL
Match: A0A6J1E9K3 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111430628 PE=3 SV=1)

HSP 1 Score: 1031.2 bits (2665), Expect = 1.7e-297
Identity = 510/589 (86.59%), Postives = 540/589 (91.68%), Query Frame = 0

Query: 6   MDLSNPHSNLSLGFSSHASPPHRPPIADDSIPIHLDSSRRESSSHSSPPVPLQLMEPQTE 65
           MDLSN HSNLSLG SSHASPPHR  IADDSI +HL+SSR+E  + SSPP+PLQLMEP TE
Sbjct: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQE-PTFSSPPIPLQLMEPLTE 60

Query: 66  SLRDDDGDA--DGDEYDDREVEEFRILGHSMCLKRRRDGQASSTLSSAKRISCEFDFETR 125
           S RD+ GDA  + D YDDR+VEEFRILGHSMCLKRRRD ++S + SS+KR SCE D ETR
Sbjct: 61  SHRDEVGDAEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSLSSSSSKRNSCERDLETR 120

Query: 126 RSVVRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHLTNKYS 185
           RS VRSWGNQ LR  DPNLFGIMEKEK+RQ KGIELIASENYVCRAVMEALGSHLTNKYS
Sbjct: 121 RSAVRSWGNQTLRATDPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYS 180

Query: 186 EGMPGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYTGLLLP 245
           EGMPGARYY GN+YIDEIETLC ERALAAFGLDSD WGVNVQPYSCTSANFAVYTGLLLP
Sbjct: 181 EGMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLP 240

Query: 246 GSRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKERALDF 305
           G RIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKL+ERALDF
Sbjct: 241 GDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDF 300

Query: 306 RPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 365
           RPKILICGGSSYPRE DY RFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS
Sbjct: 301 RPKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTS 360

Query: 366 TTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGGPHNNH 425
           TTHKSLRGPRGGIIFYRKGSKPR+RAMLLYQG+E +QYDFEEKINFAVFPSLQGGPHNNH
Sbjct: 361 TTHKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNH 420

Query: 426 IAALAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDLRSLGL 485
           IAALAVALKQVAT EYKAYMQQVKKNAQAL +ALLRK Y+LVTGGTDNHM+LWDLRSLGL
Sbjct: 421 IAALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGL 480

Query: 486 TGKNYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIADFLHAA 545
           TGKN+E+VCE+CHITLNKITIFGDNG I PGGVRIGTPAMTSRGCLESDFE I +FLH A
Sbjct: 481 TGKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIA 540

Query: 546 AQIAISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPGIET 593
           AQIAI VQRE GKLPK +L+GLQSN EVVEL  RV SFSA+FA+PG+ET
Sbjct: 541 AQIAIVVQRENGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLET 588

BLAST of Sed0004329 vs. ExPASy TrEMBL
Match: A0A6J1JAX6 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111482831 PE=3 SV=1)

HSP 1 Score: 1018.1 bits (2631), Expect = 1.5e-293
Identity = 504/587 (85.86%), Postives = 538/587 (91.65%), Query Frame = 0

Query: 6   MDLSNPHSNLSLGFSSHASPPHRPPIADDSIPIHLDSSRRESSSHSSPPVPLQLMEPQTE 65
           MDLSN +SNLSLG SSHASPPHR  IADDSI +HL+SSR+E  + SSPP+PLQLMEP TE
Sbjct: 1   MDLSNSNSNLSLGISSHASPPHRVQIADDSILLHLESSRQE-PTFSSPPIPLQLMEPLTE 60

Query: 66  SLRDDDGDADGDEYDDREVEEFRILGHSMCLKRRRDGQASSTLSSAKRISCEFDFETRRS 125
           S RD+ G A+ +  DDR+VEEF ILGHSMCLKRRRD ++S + SSAKR SCE D ETRRS
Sbjct: 61  SHRDEVGGAERE--DDRDVEEFHILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRRS 120

Query: 126 VVRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHLTNKYSEG 185
            VRSWGNQ LR ADPNLFGIMEKEK+RQ KGIELIASENYVCRAVMEALGSHLTNKYSEG
Sbjct: 121 AVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSEG 180

Query: 186 MPGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYTGLLLPGS 245
           MPGARYY GN+YIDEIETLC ERALAAFGLDSD WGVNVQPYSCTSANFAVYTGLLLPG 
Sbjct: 181 MPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPGD 240

Query: 246 RIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKERALDFRP 305
           RIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKL+ERALDFRP
Sbjct: 241 RIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFRP 300

Query: 306 KILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTT 365
           KILICGGSSYPRE DY RFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTT
Sbjct: 301 KILICGGSSYPRELDYRRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTT 360

Query: 366 HKSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGGPHNNHIA 425
           HKSLRGPRGGIIFYRKGS+PR  AMLLYQG+E++QYDFEEKINFAVFPSLQGGPHNNHIA
Sbjct: 361 HKSLRGPRGGIIFYRKGSRPRTIAMLLYQGNESNQYDFEEKINFAVFPSLQGGPHNNHIA 420

Query: 426 ALAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDLRSLGLTG 485
           ALAVALKQVAT EYKAYMQQVKKNAQAL +ALLRK Y+LVTGGTDNHM+LWDLRSLGLTG
Sbjct: 421 ALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLTG 480

Query: 486 KNYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIADFLHAAAQ 545
           KN+E+VCE+CHITLNKITIFGDNG I PGGVRIGTPAMTSRGCLESDFE I +FLH AAQ
Sbjct: 481 KNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAAQ 540

Query: 546 IAISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPGIET 593
           IA+ VQRE GKLPKP+L+GLQS+ EVVEL  RV SFSA+FA+PG+ET
Sbjct: 541 IAVVVQREHGKLPKPFLRGLQSSNEVVELGYRVASFSAKFAMPGLET 584

BLAST of Sed0004329 vs. TAIR 10
Match: AT1G36370.1 (serine hydroxymethyltransferase 7 )

HSP 1 Score: 823.9 bits (2127), Expect = 8.1e-239
Identity = 413/592 (69.76%), Postives = 477/592 (80.57%), Query Frame = 0

Query: 6   MDLSNPHSNLSLGFS-SHAS----PPHRPPIADDSIPIHLDSSRRESSSHSSPPVPLQLM 65
           MDLS   +N  LGF  SHAS    P  R PIADDSI + +D S R   +  S P+PLQL+
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFS-PIPLQLL 60

Query: 66  EPQTESLR--DDDGDADGDEYDDREVEEFRILGHSMCLKRRRDGQASSTLSS-AKRISC- 125
           E + E     D+     G   D +E E FRILGH MCLKR+RD     T S   KR S  
Sbjct: 61  EQKAEKTTTVDEPKKDGGGGGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSIG 120

Query: 126 EFDFETRRSVVRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGS 185
           + D E+RR+ VR+WG+Q + +ADP++  +MEKEK+RQ +GIELIASEN+VCRAVMEALGS
Sbjct: 121 DSDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGS 180

Query: 186 HLTNKYSEGMPGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAV 245
           HLTNKYSEGMPGARYY GN+YID+IE LC ERAL AFGL+SD WGVNVQPYSCTSANFAV
Sbjct: 181 HLTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAV 240

Query: 246 YTGLLLPGSRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKL 305
           YTGLLLPG RIMGLD+PSGG+ SHGYCTP G+K+S ASIFFES PYKVNPQTGYIDYDKL
Sbjct: 241 YTGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKL 300

Query: 306 KERALDFRPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFG 365
           +++ALD+RPKILICGGSSYPR+WD+AR RQIADKCGAVLMCDMA ISGLVA KEC +PF 
Sbjct: 301 EDKALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFD 360

Query: 366 YCDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQ 425
           +CD+VTSTTHK LRGPRGGIIFYR+G K RK+         +  YD EEKINFAVFPSLQ
Sbjct: 361 HCDIVTSTTHKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQ 420

Query: 426 GGPHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLW 485
           GGPHNNHIAALA+ALKQVATPEYKAY+QQ+KKNAQAL AALLR+K RLVTGGTDNH+LLW
Sbjct: 421 GGPHNNHIAALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLW 480

Query: 486 DLRSLGLTGKNYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETI 545
           DL  +GLTGK YEKVCE+CHITLNK  IFGDNGTI PGGVRIGTPAMT+RGC+ESDFET+
Sbjct: 481 DLTPMGLTGKVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETM 540

Query: 546 ADFLHAAAQIAISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALP 589
           ADFL  AAQI  ++QRE GK  K ++K L +N ++ EL+NRVE+F+ Q+ +P
Sbjct: 541 ADFLIKAAQITSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

BLAST of Sed0004329 vs. TAIR 10
Match: AT1G22020.1 (serine hydroxymethyltransferase 6 )

HSP 1 Score: 813.1 bits (2099), Expect = 1.4e-235
Identity = 406/593 (68.47%), Postives = 482/593 (81.28%), Query Frame = 0

Query: 13  SNLSLGF-SSHASP-PHRP--PIADDSIPIHLDSSRRESSSHSSPPVPLQLMEPQTESL- 72
           S+LSLGF SSHA P PH P  PIADDSI + +DSS R  SS+  PPVPLQL+E + +   
Sbjct: 7   SDLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFR-PSSNPMPPVPLQLLEQRFDVTG 66

Query: 73  -------RDDDGDADGDEYDDREVEEFRILGHSMCLKRRRDGQASSTLSSA--KRISCEF 132
                   DD+   D D+ D RE E+F +LGH M LKR R G + S  SS+  KR   + 
Sbjct: 67  SCSRVVEEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVVDS 126

Query: 133 DFETRRSVVRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHL 192
             E+RR+VVR+WGNQ +  ADP +   MEKEK+RQF+GIELIASEN+VCRAVMEALGSHL
Sbjct: 127 GIESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHL 186

Query: 193 TNKYSEGMPGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYT 252
           TNKYSEGMPGARYY GN+YID+IE LC ERALAAFGL+ + WGVNVQPYSCTSANFAV+T
Sbjct: 187 TNKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFT 246

Query: 253 GLLLPGSRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKE 312
           GLL+PG RIMGLD+PSGG+ SHGY TP G+KVSGASIFFES PYKV+P+TGYIDYDKL+E
Sbjct: 247 GLLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEE 306

Query: 313 RALDFRPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYC 372
           +ALD+RPKILICGGSSYPR+W++ RFR IADKCGAVLM DMAQISGLVAAKE  +PF YC
Sbjct: 307 KALDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYC 366

Query: 373 DVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGG 432
           D+VTSTTHKSLRGPRGGIIFY++G KP+K+++ L       QYDFEEKINF+VFPSLQGG
Sbjct: 367 DIVTSTTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGG 426

Query: 433 PHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDL 492
           PHNNHIAALA+ALKQ A+PEYK YM+QVKKNA+AL +AL+ +K +L+TGGTDNH+LLWDL
Sbjct: 427 PHNNHIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDL 486

Query: 493 RSLGLTGKNYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIAD 552
             LGLTGK YEKVCE+CHIT+NK+ IF +NG I PGGVRIG+PAMTSRGCLE +FET+AD
Sbjct: 487 TPLGLTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMAD 546

Query: 553 FLHAAAQIAISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPGIE 592
           FL+ AAQIA + QRE GKL K  LK +    E+ +L+N+VE+F+ QFA+P  +
Sbjct: 547 FLYRAAQIASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFD 598

BLAST of Sed0004329 vs. TAIR 10
Match: AT4G13930.1 (serine hydroxymethyltransferase 4 )

HSP 1 Score: 614.0 bits (1582), Expect = 1.3e-175
Identity = 296/463 (63.93%), Postives = 353/463 (76.24%), Query Frame = 0

Query: 127 VRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 186
           V SWGN  L   DP +  ++EKEKRRQ +GIELIASEN+   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 187 PGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYTGLLLPGSR 246
           PG RYYGGN +IDEIE LC  RAL AF  D   WGVNVQPYS + ANFA YT LL P  R
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 247 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKERALDFRPK 306
           IMGLD PSGG+ +HGY T  G+K+S  SI+FESLPYKVN  TGYIDYDKL+E+ALDFRPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 307 ILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 366
           +LICGGS+YPR+WDYARFR IADK GA+L+CDMA ISGLVAA+E  +PF YCDVVT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 367 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGGPHNNHIAA 426
           KSLRGPR G+IFYRKG KP K+        E   YDFE+KINFAVFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 427 LAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDLRSLGLTGK 486
           LAVALKQ  TP +K Y +QVK NA AL   L+ K Y++VT GT+NH++LWDLR LGLTG 
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 487 NYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIADFLHAAAQI 546
             EK+C++C ITLNK  +FGD+  + PGGVRIG PAMTSRG +E DFE I +FL  A  +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 547 AISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPG 590
            + +Q+  GKL K + KGL +N ++ +L+  VE FSA + +PG
Sbjct: 424 TLDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPG 461

BLAST of Sed0004329 vs. TAIR 10
Match: AT4G13890.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 605.1 bits (1559), Expect = 5.9e-173
Identity = 286/463 (61.77%), Postives = 348/463 (75.16%), Query Frame = 0

Query: 127 VRSWGNQMLRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 186
           V SWGN  L   DP ++ ++EKEK RQ +GIELIA+EN+   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 187 PGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVYTGLLLPGSR 246
           PG RYYGG  +IDEIE+LC  R+L AF  + + WGVNVQPYS + ANFA YT LL P  R
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 247 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLKERALDFRPK 306
           IMGLD PSGG+ +HGY +  G+ +S  SI+FE+LPYKV+ +TGYIDYDKL+E+A+DFRPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 307 ILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 366
           ++ICGG+SYPREWDYARFR +ADK GA L+CDMA  S LVAA+E   PF YCDVVT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 367 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQGGPHNNHIAA 426
           KSLRGPR G+IFYRKG KP K+        E + YDF+ KIN AVFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKGQ-----PEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 427 LAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWDLRSLGLTGK 486
           LAVALKQV  P +K Y +QVK NA  L + L+ K Y LVT GTDNH++LWDLR LGLTG 
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 487 NYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIADFLHAAAQI 546
             EKVCE+C+ITLN+  +FGD   + PGGVRIGTPAMTSRG +E DFE I +FLH A  I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 547 AISVQRECGKLPKPYLKGLQSNTEVVELQNRVESFSAQFALPG 590
            + +Q + GK+ K + KGL +N E+ E++  VE F+  F +PG
Sbjct: 424 TLDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPG 461

BLAST of Sed0004329 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 489.6 bits (1259), Expect = 3.6e-138
Identity = 252/479 (52.61%), Postives = 328/479 (68.48%), Query Frame = 0

Query: 121 ETRRSVVRSWGNQM---LRVADPNLFGIMEKEKRRQFKGIELIASENYVCRAVMEALGSH 180
           E  RS V +W  Q+   L   DP +  I+E EK RQ+KG+ELI SEN+   +VM+A+GS 
Sbjct: 39  EKERSRV-TWPKQLNAPLEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSV 98

Query: 181 LTNKYSEGMPGARYYGGNRYIDEIETLCCERALAAFGLDSDDWGVNVQPYSCTSANFAVY 240
           +TNKYSEG PGARYYGGN YID  ETLC +RAL AF LD + WGVNVQP S + ANF VY
Sbjct: 99  MTNKYSEGYPGARYYGGNEYIDMAETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVY 158

Query: 241 TGLLLPGSRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLK 300
           T LL P  RIM LD P GG+ SHGY T + +K+S  SIFFE++PY+++  TGYIDYD+++
Sbjct: 159 TALLKPHERIMALDLPHGGHLSHGYQT-DTKKISAVSIFFETMPYRLDESTGYIDYDQME 218

Query: 301 ERALDFRPKILICGGSSYPREWDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGY 360
           + A  FRPK+++ G S+Y R +DYAR R++ +K  AV++ DMA ISGLVAA    SPF Y
Sbjct: 219 KSATLFRPKLIVAGASAYARLYDYARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDY 278

Query: 361 CDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGSETDQYDFEEKINFAVFPSLQG 420
            DVVT+TTHKSLRGPRG +IF+RKG K   +     QG E   YDFE+KIN AVFP LQG
Sbjct: 279 ADVVTTTTHKSLRGPRGAMIFFRKGVKEINK-----QGKEV-LYDFEDKINQAVFPGLQG 338

Query: 421 GPHNNHIAALAVALKQVATPEYKAYMQQVKKNAQALVAALLRKKYRLVTGGTDNHMLLWD 480
           GPHN+ I  LAVALKQ  T EYKAY +QV  N+      L+ + Y LV+GGTDNH++L +
Sbjct: 339 GPHNHTITGLAVALKQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVN 398

Query: 481 LRSLGLTGKNYEKVCEICHITLNKITIFGDNGTIIPGGVRIGTPAMTSRGCLESDFETIA 540
           L+  G+ G   EKV E  HI  NK T+ GD   ++PGG+R+GTPA+TSRG +E DF  +A
Sbjct: 399 LKPKGIDGSRVEKVLEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVA 458

Query: 541 DFLHAAAQIAISVQREC-GKLPKPYLKGLQSN----TEVVELQNRVESFSAQFALPGIE 592
           ++   A  IA+ V+ E  G   K ++  ++S+    +E+ +L++ VE F+ QF   G E
Sbjct: 459 EYFDKAVTIALKVKSEAQGTKLKDFVSAMESSSTIQSEIAKLRHEVEEFAKQFPTIGFE 509