Sed0004039 (gene) Chayote v1

Overview
NameSed0004039
Typegene
OrganismSechium edule (Chayote v1)
DescriptionGDSL esterase/lipase 1-like
LocationLG06: 46385975 .. 46387362 (+)
RNA-Seq ExpressionSed0004039
SyntenySed0004039
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAATTCATCAATCTTGTGCTTATGTTTGTTCATCTCTTTTGCTGCTGTTGGCTCTGCTTGCGACCCATTTCCTATGTTCGTTTTCGGAGATTCCATTTTCGATACTGGAAACAACAATTACATCCGCACCAATCCTACTGCCCAAGCAAATTACTGGCCCTATGGCCAAAATTTCTTCAAATATCCCACAGGAAGATGGTCCGATGCCAGAATTGTTCCTGATTTCTTTGGTACAACCATTGACAACTTTCAATCATTTCTAAACACATCTATTCATCTTCAAGTTTGTTCTCTTTTCTTAATGTTGCAGCTGAATATGCAAATTTGCGATTGGTTCTTCCATACCTTCATCCATGGAATCAGCATTACATGGATGGGGCAAATTTTGCATCAGGTGGAGCAGGGGCTCTCGATCAAATCAATCGAGGATTCGTATGTTCACACATTCCTTCCTTTACACTCTTTATCGTAGATTAATATTAATTAATACTGTATGTTTCAGGTAATAAGCCTCAATACTCAGCTAAGAAATTTCAACAAAGTGGAGAGGATACTGAGAAAGCAACTTGGATATTCACAAGCAAAGGCATTGCTATCAAAAGGTGTTTACTTATTCTCAATTGGGACCAACGATTACCGCACCTTTGCACAAGACCCCAACCTTTTCAACTCCTACTCATTGCAAGAGTATGTTGATTGGGTGATTGGGAACTTGACTACTGTCATACAAGTAACATGAATCAATCTCTTTTCAATCTTGTTTGATCACCATTTAATTTTTATTAATCTCGAGTCTCGTATAACATGGAACAGGAAATCCATAAGAAAGGAGGAAGGAAATTCGTTATGCTAAACATTTGGTCTCCTAATCATGTACCTGCTGTGCAAGAAGAAGTGGCATCCCGAGGCAAAGATGCTCGGCCCGAAGTGCTTAATTATCTAATAGAATTGCACAATCGACAACTTTACGGAGCACTGCAAAAGCTCACCACAAGACTCAAAGGATTCAGATATTCCTATGCTGATTCCTACAAAGTTTTCAATGAAATGGTCAGCAACCCTGCAAAATATGGTACGTAAATTTCAAGAATCTTGTAGCAATTTTAATGTGGGTTTCTGATTCAGACTGGTTTGTACTGGGAAACAGGACTCAAAGAAGTGAATGTTGCGTGCTGTGGAAGTGGGAGATTTAGAGGAATTCAAAGCTGTGGAGGAAAGGGTGATATAAAAGAATATGAATTATGTGGGAATCCTAGAGAGCATTTGTTCTATGATGCAAACCATGGAAGTGATAGAGGATATCAAATACTTGCACAGATGATATGGAATGGGGATTCTAATAGTTCAAGGCCAGTAAATGTCAAATCTTTGTTTCAATCCTGA

mRNA sequence

ATGGCGAATTCATCAATCTTGTGCTTATGTTTGTTCATCTCTTTTGCTGCTGTTGGCTCTGCTTGCGACCCATTTCCTATGTTCGTTTTCGGAGATTCCATTTTCGATACTGGAAACAACAATTACATCCGCACCAATCCTACTGCCCAAGCAAATTACTGGCCCTATGGCCAAAATTTCTTCAAATATCCCACAGGAAGATGGTCCGATGCCAGAATTGTTCCTGATTTCTTTGCTGAATATGCAAATTTGCGATTGGTTCTTCCATACCTTCATCCATGGAATCAGCATTACATGGATGGGGCAAATTTTGCATCAGGTGGAGCAGGGGCTCTCGATCAAATCAATCGAGGATTCGTAATAAGCCTCAATACTCAGCTAAGAAATTTCAACAAAGTGGAGAGGATACTGAGAAAGCAACTTGGATATTCACAAGCAAAGGCATTGCTATCAAAAGGTGTTTACTTATTCTCAATTGGGACCAACGATTACCGCACCTTTGCACAAGACCCCAACCTTTTCAACTCCTACTCATTGCAAGAGTATGTTGATTGGGTGATTGGGAACTTGACTACTGTCATACAAGAAATCCATAAGAAAGGAGGAAGGAAATTCGTTATGCTAAACATTTGGTCTCCTAATCATGTACCTGCTGTGCAAGAAGAAGTGGCATCCCGAGGCAAAGATGCTCGGCCCGAAGTGCTTAATTATCTAATAGAATTGCACAATCGACAACTTTACGGAGCACTGCAAAAGCTCACCACAAGACTCAAAGGATTCAGATATTCCTATGCTGATTCCTACAAAGTTTTCAATGAAATGGTCAGCAACCCTGCAAAATATGGACTCAAAGAAGTGAATGTTGCGTGCTGTGGAAGTGGGAGATTTAGAGGAATTCAAAGCTGTGGAGGAAAGGGTGATATAAAAGAATATGAATTATGTGGGAATCCTAGAGAGCATTTGTTCTATGATGCAAACCATGGAAGTGATAGAGGATATCAAATACTTGCACAGATGATATGGAATGGGGATTCTAATAGTTCAAGGCCAGTAAATGTCAAATCTTTGTTTCAATCCTGA

Coding sequence (CDS)

ATGGCGAATTCATCAATCTTGTGCTTATGTTTGTTCATCTCTTTTGCTGCTGTTGGCTCTGCTTGCGACCCATTTCCTATGTTCGTTTTCGGAGATTCCATTTTCGATACTGGAAACAACAATTACATCCGCACCAATCCTACTGCCCAAGCAAATTACTGGCCCTATGGCCAAAATTTCTTCAAATATCCCACAGGAAGATGGTCCGATGCCAGAATTGTTCCTGATTTCTTTGCTGAATATGCAAATTTGCGATTGGTTCTTCCATACCTTCATCCATGGAATCAGCATTACATGGATGGGGCAAATTTTGCATCAGGTGGAGCAGGGGCTCTCGATCAAATCAATCGAGGATTCGTAATAAGCCTCAATACTCAGCTAAGAAATTTCAACAAAGTGGAGAGGATACTGAGAAAGCAACTTGGATATTCACAAGCAAAGGCATTGCTATCAAAAGGTGTTTACTTATTCTCAATTGGGACCAACGATTACCGCACCTTTGCACAAGACCCCAACCTTTTCAACTCCTACTCATTGCAAGAGTATGTTGATTGGGTGATTGGGAACTTGACTACTGTCATACAAGAAATCCATAAGAAAGGAGGAAGGAAATTCGTTATGCTAAACATTTGGTCTCCTAATCATGTACCTGCTGTGCAAGAAGAAGTGGCATCCCGAGGCAAAGATGCTCGGCCCGAAGTGCTTAATTATCTAATAGAATTGCACAATCGACAACTTTACGGAGCACTGCAAAAGCTCACCACAAGACTCAAAGGATTCAGATATTCCTATGCTGATTCCTACAAAGTTTTCAATGAAATGGTCAGCAACCCTGCAAAATATGGACTCAAAGAAGTGAATGTTGCGTGCTGTGGAAGTGGGAGATTTAGAGGAATTCAAAGCTGTGGAGGAAAGGGTGATATAAAAGAATATGAATTATGTGGGAATCCTAGAGAGCATTTGTTCTATGATGCAAACCATGGAAGTGATAGAGGATATCAAATACTTGCACAGATGATATGGAATGGGGATTCTAATAGTTCAAGGCCAGTAAATGTCAAATCTTTGTTTCAATCCTGA

Protein sequence

MANSSILCLCLFISFAAVGSACDPFPMFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQNFFKYPTGRWSDARIVPDFFAEYANLRLVLPYLHPWNQHYMDGANFASGGAGALDQINRGFVISLNTQLRNFNKVERILRKQLGYSQAKALLSKGVYLFSIGTNDYRTFAQDPNLFNSYSLQEYVDWVIGNLTTVIQEIHKKGGRKFVMLNIWSPNHVPAVQEEVASRGKDARPEVLNYLIELHNRQLYGALQKLTTRLKGFRYSYADSYKVFNEMVSNPAKYGLKEVNVACCGSGRFRGIQSCGGKGDIKEYELCGNPREHLFYDANHGSDRGYQILAQMIWNGDSNSSRPVNVKSLFQS
Homology
BLAST of Sed0004039 vs. NCBI nr
Match: XP_038903481.1 (GDSL esterase/lipase 1-like [Benincasa hispida])

HSP 1 Score: 540.8 bits (1392), Expect = 8.9e-150
Identity = 263/371 (70.89%), Postives = 305/371 (82.21%), Query Frame = 0

Query: 1   MANSS--ILCLCLFISFAAVGSAC---------DPFPMFVFGDSIFDTGNNNYIRTNPTA 60
           MANS+   LC+ L + F  VG               P+FVFGDSI DTGNNNYI T PTA
Sbjct: 1   MANSNNWSLCVLLLLIFYPVGECVVCKQDELLQQHIPLFVFGDSIIDTGNNNYINTIPTA 60

Query: 61  QANYWPYGQNFFKYPTGRWSDARIVPDFFAEYANLRLVLPYLHPWNQHYMDGANFASGGA 120
           Q+NY P+GQ FFKYP+GRWSD R+VPDFFAEYANL LV PYL+P N+ Y+DG NFASGGA
Sbjct: 61  QSNYPPFGQTFFKYPSGRWSDGRVVPDFFAEYANLPLVFPYLYPGNKRYIDGINFASGGA 120

Query: 121 GALDQINRGF-VISLNTQLRNFNKVERILRKQLGYSQAKALLSKGVYLFSIGTNDYRTFA 180
           GALD++NRGF VISL TQ ++FNKVERILRKQLG +QAK LLS+GVYL +IGTNDYRTFA
Sbjct: 121 GALDEVNRGFGVISLKTQAKSFNKVERILRKQLGKTQAKMLLSRGVYLITIGTNDYRTFA 180

Query: 181 QDPNLFNSYSLQEYVDWVIGNLTTVIQEIHKKGGRKFVMLNIWSPNHVPAVQEEVASRGK 240
            D  LF+SYS++EYVD VI NLT+VI++IHKKGGRKFV +N+WS NHVPAV E VAS+GK
Sbjct: 181 SDSKLFDSYSVEEYVDLVIENLTSVIKDIHKKGGRKFVAMNLWSYNHVPAVLEAVASQGK 240

Query: 241 DARPEVLNYLIELHNRQLYGALQKLTTRLKGFRYSYADSYKVFNEMVSNPAKYGLKEVNV 300
            AR E LN L+ELHN+QLY ALQKL T+L+GFRYSY DSYKVF E+ SNPAKYG KEV  
Sbjct: 241 YARLEQLNQLVELHNKQLYKALQKLATKLEGFRYSYVDSYKVFEEITSNPAKYGFKEVKD 300

Query: 301 ACCGSGRFRGIQSCGGKGDIKEYELCGNPREHLFYDANHGSDRGYQILAQMIWNGDSNSS 360
           ACCGSG+++GIQSCGG GD+KEYELCGNP+EHLF+D+NHGSDR YQILA+M WNGDS++S
Sbjct: 301 ACCGSGKYKGIQSCGGMGDVKEYELCGNPKEHLFFDSNHGSDRAYQILAEMAWNGDSSTS 360

BLAST of Sed0004039 vs. NCBI nr
Match: XP_004144442.1 (GDSL esterase/lipase 1 [Cucumis sativus] >KAE8650888.1 hypothetical protein Csa_000991 [Cucumis sativus])

HSP 1 Score: 536.6 bits (1381), Expect = 1.7e-148
Identity = 259/367 (70.57%), Postives = 305/367 (83.11%), Query Frame = 0

Query: 1   MANSSILCLCLFISFA--AVG---SAC---DPFPMFVFGDSIFDTGNNNYIRTNPTAQAN 60
           MAN   LCLCL + F   +VG   S C      P+FVFGDSI D GNNNYI TN  AQ+N
Sbjct: 1   MANLKDLCLCLLLLFIFFSVGDCISVCRQDHNVPLFVFGDSIVDIGNNNYINTNSIAQSN 60

Query: 61  YWPYGQNFFKYPTGRWSDARIVPDFFAEYANLRLVLPYLHPWNQHYMDGANFASGGAGAL 120
           Y PYG  FFKYP+GRWSD R+VPDFFA+YANL+L+LPYL+P N+ Y+DG NFASGGAGAL
Sbjct: 61  YPPYGLTFFKYPSGRWSDGRVVPDFFAQYANLQLLLPYLYPGNKRYIDGINFASGGAGAL 120

Query: 121 DQINRGFVISLNTQLRNFNKVERILRKQLGYSQAKALLSKGVYLFSIGTNDYRTFAQDPN 180
           D+INRG VISL TQ R+F KVE+ILRKQLG +QAK LLS+ VYL S+GTNDYRTFA D  
Sbjct: 121 DEINRGLVISLKTQARSFKKVEKILRKQLGKTQAKTLLSRAVYLISVGTNDYRTFASDSK 180

Query: 181 LFNSYSLQEYVDWVIGNLTTVIQEIHKKGGRKFVMLNIWSPNHVPAVQEEVASRGKDARP 240
           LF+SYS++EYVD VIGNLT+VI+EI+K GGRKFV++N+WS NHVPAV E VAS+GKDA+ 
Sbjct: 181 LFDSYSIEEYVDLVIGNLTSVIKEIYKNGGRKFVVMNLWSFNHVPAVLEAVASQGKDAQL 240

Query: 241 EVLNYLIELHNRQLYGALQKLTTRLKGFRYSYADSYKVFNEMVSNPAKYGLKEVNVACCG 300
           + LN L+E+HN+QLY ALQKLTT L+GFRYSY DSYKVF E+ +NPAK+GLKEV  ACCG
Sbjct: 241 KQLNQLVEMHNKQLYKALQKLTTELQGFRYSYVDSYKVFEEITTNPAKHGLKEVKSACCG 300

Query: 301 SGRFRGIQSCGGKGDIKEYELCGNPREHLFYDANHGSDRGYQILAQMIWNGDSNSSRPVN 360
           SG +RGIQSCGGKGD+KEYELC NP+EHLF+D+NHGS++ YQILA+M WNGDSN+S PVN
Sbjct: 301 SGIYRGIQSCGGKGDVKEYELCKNPKEHLFFDSNHGSEKAYQILAEMAWNGDSNTSTPVN 360

BLAST of Sed0004039 vs. NCBI nr
Match: XP_008460337.1 (PREDICTED: GDSL esterase/lipase 1-like [Cucumis melo])

HSP 1 Score: 525.0 bits (1351), Expect = 5.0e-145
Identity = 244/334 (73.05%), Postives = 290/334 (86.83%), Query Frame = 0

Query: 26  PMFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQNFFKYPTGRWSDARIVPDFFAEYANLR 85
           P+FVFGDSI D GNNNYI T P A++NY PYG  FFK P+GRWSD R+VPDFFA+YANL+
Sbjct: 37  PLFVFGDSIVDIGNNNYINTIPIARSNYPPYGLTFFKCPSGRWSDGRVVPDFFAQYANLQ 96

Query: 86  LVLPYLHPWNQHYMDGANFASGGAGALDQINRGFVISLNTQLRNFNKVERILRKQLGYSQ 145
           L+LPYL+P N+HY++G NFASGGAGALD+INRG VISL TQ R+F KVE+ILRKQ+G +Q
Sbjct: 97  LLLPYLYPGNKHYINGINFASGGAGALDEINRGLVISLKTQARSFKKVEKILRKQIGKTQ 156

Query: 146 AKALLSKGVYLFSIGTNDYRTFAQDPNLFNSYSLQEYVDWVIGNLTTVIQEIHKKGGRKF 205
           AKALLS+ VYL SIGTNDYRTFA +  LF+SYS +EYVD VIGNLT+VI+EI+K GGRKF
Sbjct: 157 AKALLSRAVYLISIGTNDYRTFASNSKLFDSYSFEEYVDLVIGNLTSVIKEIYKNGGRKF 216

Query: 206 VMLNIWSPNHVPAVQEEVASRGKDARPEVLNYLIELHNRQLYGALQKLTTRLKGFRYSYA 265
           V++N+WS NHVPAV E VAS+GKDA+ E LN L+E+HN+QLY A+QKLTT L+GFRYSY 
Sbjct: 217 VVMNLWSFNHVPAVLEAVASQGKDAQLEQLNQLVEMHNKQLYKAVQKLTTELQGFRYSYV 276

Query: 266 DSYKVFNEMVSNPAKYGLKEVNVACCGSGRFRGIQSCGGKGDIKEYELCGNPREHLFYDA 325
           DSYKVF E+ +NPAK+GLKEV  ACCGSG +RGIQSCGGKG++KEYELCGNP+EHLF+D+
Sbjct: 277 DSYKVFEEITNNPAKHGLKEVKNACCGSGVYRGIQSCGGKGNVKEYELCGNPKEHLFFDS 336

Query: 326 NHGSDRGYQILAQMIWNGDSNSSRPVNVKSLFQS 360
           NHGSD+ YQILA+M WNGD N+S PVNVKSLF S
Sbjct: 337 NHGSDKAYQILAEMAWNGDLNASTPVNVKSLFLS 370

BLAST of Sed0004039 vs. NCBI nr
Match: KAA0053544.1 (GDSL esterase/lipase 1-like [Cucumis melo var. makuwa] >TYK19139.1 GDSL esterase/lipase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 525.0 bits (1351), Expect = 5.0e-145
Identity = 244/334 (73.05%), Postives = 290/334 (86.83%), Query Frame = 0

Query: 26  PMFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQNFFKYPTGRWSDARIVPDFFAEYANLR 85
           P+FVFGDSI D GNNNYI T P A++NY PYG  FFK P+GRWSD R+VPDFFA+YANL+
Sbjct: 12  PLFVFGDSIVDIGNNNYINTIPIARSNYPPYGLTFFKCPSGRWSDGRVVPDFFAQYANLQ 71

Query: 86  LVLPYLHPWNQHYMDGANFASGGAGALDQINRGFVISLNTQLRNFNKVERILRKQLGYSQ 145
           L+LPYL+P N+HY++G NFASGGAGALD+INRG VISL TQ R+F KVE+ILRKQ+G +Q
Sbjct: 72  LLLPYLYPGNKHYINGINFASGGAGALDEINRGLVISLKTQARSFKKVEKILRKQIGKTQ 131

Query: 146 AKALLSKGVYLFSIGTNDYRTFAQDPNLFNSYSLQEYVDWVIGNLTTVIQEIHKKGGRKF 205
           AKALLS+ VYL SIGTNDYRTFA +  LF+SYS +EYVD VIGNLT+VI+EI+K GGRKF
Sbjct: 132 AKALLSRAVYLISIGTNDYRTFASNSKLFDSYSFEEYVDLVIGNLTSVIKEIYKNGGRKF 191

Query: 206 VMLNIWSPNHVPAVQEEVASRGKDARPEVLNYLIELHNRQLYGALQKLTTRLKGFRYSYA 265
           V++N+WS NHVPAV E VAS+GKDA+ E LN L+E+HN+QLY A+QKLTT L+GFRYSY 
Sbjct: 192 VVMNLWSFNHVPAVLEAVASQGKDAQLEQLNQLVEMHNKQLYKAVQKLTTELQGFRYSYV 251

Query: 266 DSYKVFNEMVSNPAKYGLKEVNVACCGSGRFRGIQSCGGKGDIKEYELCGNPREHLFYDA 325
           DSYKVF E+ +NPAK+GLKEV  ACCGSG +RGIQSCGGKG++KEYELCGNP+EHLF+D+
Sbjct: 252 DSYKVFEEITNNPAKHGLKEVKNACCGSGVYRGIQSCGGKGNVKEYELCGNPKEHLFFDS 311

Query: 326 NHGSDRGYQILAQMIWNGDSNSSRPVNVKSLFQS 360
           NHGSD+ YQILA+M WNGD N+S PVNVKSLF S
Sbjct: 312 NHGSDKAYQILAEMAWNGDLNASTPVNVKSLFLS 345

BLAST of Sed0004039 vs. NCBI nr
Match: XP_022980654.1 (GDSL esterase/lipase 1-like [Cucurbita maxima])

HSP 1 Score: 523.1 bits (1346), Expect = 1.9e-144
Identity = 250/368 (67.93%), Postives = 297/368 (80.71%), Query Frame = 0

Query: 1   MANSSILCLCLFISFAAVGSAC--------DPFPMFVFGDSIFDTGNNNYIRTNPTAQAN 60
           MA+S+ L LC+   F AVG  C           P+F FGDS+ DTGNNNYI T   AQAN
Sbjct: 1   MASSTNLRLCVLFIFFAVGCVCMQDEQLHNHHGPLFTFGDSLVDTGNNNYINTRRIAQAN 60

Query: 61  YWPYGQNFFKYPTGRWSDARIVPDFFAEYANLRLVLPYLHPWNQHYMDGANFASGGAGAL 120
           Y PYGQ FFKYP+GRWSD R++PDFFAEYA+L  +LPYLHP N+ Y+ G NFASGGAGAL
Sbjct: 61  YPPYGQTFFKYPSGRWSDGRVLPDFFAEYADLPWILPYLHPGNKRYVYGTNFASGGAGAL 120

Query: 121 DQINRGFVISLNTQLRNFNKVERILRKQLGYSQAKALLSKGVYLFSIGTNDYRTFAQDPN 180
            + N+G V+SL TQ RNF KVERILRKQLG +    +LSKGVYL  IGTNDY  +A D  
Sbjct: 121 HETNQGLVMSLKTQARNFKKVERILRKQLGRTGVNTILSKGVYLIWIGTNDYSVYASDSK 180

Query: 181 LFNSYSLQEYVDWVIGNLTTVIQEIHKKGGRKFVMLNIWSPNHVPAVQEE-VASRGKDAR 240
           LF+SYSL++YVD VIGNL++VI+EIHKKGGRKFV++N+WSPNH+P VQEE VAS+G+DAR
Sbjct: 181 LFSSYSLEKYVDLVIGNLSSVIEEIHKKGGRKFVVINLWSPNHIPLVQEEAVASQGRDAR 240

Query: 241 PEVLNYLIELHNRQLYGALQKLTTRLKGFRYSYADSYKVFNEMVSNPAKYGLKEVNVACC 300
              LN+L+ELHN QLY  LQ+LTT+LKGFRYSYADSYKV  E+ SNPAKYG K+V VACC
Sbjct: 241 LGQLNHLVELHNGQLYKELQRLTTKLKGFRYSYADSYKVVEEIASNPAKYGFKDVKVACC 300

Query: 301 GSGRFRGIQSCGGKGDIKEYELCGNPREHLFYDANHGSDRGYQILAQMIWNGDSNSSRPV 360
           GSG+ RGIQSCGG+  +KEY+LC NP+EHLF+D+NHGSD+GYQILA+MIWNGD N+SRP+
Sbjct: 301 GSGKLRGIQSCGGQDTLKEYQLCENPKEHLFFDSNHGSDKGYQILAEMIWNGDLNTSRPI 360

BLAST of Sed0004039 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 2.0e-83
Identity = 157/336 (46.73%), Postives = 222/336 (66.07%), Query Frame = 0

Query: 27  MFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQNFFKYPTGRWSDARIVPDFFAEYANLRL 86
           +FVFGDS+FD GNNNYI T  + ++NYWPYGQ  FK PTGR SD R++PDF AEYA L L
Sbjct: 38  LFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLPL 97

Query: 87  VLPYLHPW--NQHYMDGANFASGGAGALDQINRGFVISLNTQLRNFNKVERILRKQLGYS 146
           + P L P+  N  +  G NFASGGAGAL     G VI+L TQL NF KVE +LR +LG +
Sbjct: 98  IPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLVINLRTQLNNFKKVEEMLRSKLGDA 157

Query: 147 QAKALLSKGVYLFSIGTNDYR-TFAQDPNLFNSYSLQEYVDWVIGNLTTVIQEIHKKGGR 206
           + K ++S+ VYLF IG NDY+  F  + +LF S S ++YVD+V+GN+T V +E++  GGR
Sbjct: 158 EGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSISNEKYVDYVVGNMTDVFKEVYNLGGR 217

Query: 207 KFVMLNIWSPNHVPAVQEEVASRGKDARPEVLNYLIELHNRQLYGALQKLTTRLKGFRYS 266
           KF +LN    +  PA      ++ +     V   LI +HN +L   L++L   L GF+Y+
Sbjct: 218 KFGILNTGPYDCAPASLVIDQTKIRSCFQPVTE-LINMHNEKLLNGLRRLNHELSGFKYA 277

Query: 267 YADSYKVFNEMVSNPAKYGLKEVNVACCGSGRFRGIQSCGGK-GDIKEYELCGNPREHLF 326
             D +   +E +++P+KYG KE   ACCGSG  RGI +CGG+ G  + YELC N  ++LF
Sbjct: 278 LHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLF 337

Query: 327 YDANHGSDRGYQILAQMIWNGDSNSSRPVNVKSLFQ 359
           +D  H +++  + +A++IW+G +N + P N+K+LF+
Sbjct: 338 FDPFHLTEKANRQIAELIWSGPTNITGPYNLKALFE 372

BLAST of Sed0004039 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 9.7e-83
Identity = 156/337 (46.29%), Postives = 225/337 (66.77%), Query Frame = 0

Query: 27  MFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQNFFKYPTGRWSDARIVPDFFAEYANLRL 86
           +FVFGDS+FD GNNNYI T P+ ++NYWPYGQ  FK+PTGR SD R +PDF AEYA L L
Sbjct: 40  LFVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPL 99

Query: 87  VLPYLHPWN--QHYMDGANFASGGAGALDQINRGFVISLNTQLRNFNKVERILRKQLGYS 146
           +  YL P N    +  G +FAS GAGAL     G VI+L +QL NF KVE++LR  LG +
Sbjct: 100 IPAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEA 159

Query: 147 QAKALLSKGVYLFSIGTNDYR-TFAQDPNLFNSYSLQEYVDWVIGNLTTVIQEIHKKGGR 206
           Q K ++S+ VYLF IG NDY+  F+ + ++F S   + YVD+V+GN T VI+E++K GGR
Sbjct: 160 QGKMVISRAVYLFHIGVNDYQYPFSTNSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGGR 219

Query: 207 KFVMLNIWSPNHVPA-VQEEVASRGKDARPEVLNYLIELHNRQLYGALQKLTTRLKGFRY 266
           KF  LN+ + +  PA +  +    G   +P  +  LI LHN +L   L++L   L GF+Y
Sbjct: 220 KFGFLNMGAYDCAPASLIIDQTKIGTCFKP--VTELINLHNEKLESGLRRLERELSGFKY 279

Query: 267 SYADSYKVFNEMVSNPAKYGLKEVNVACCGSGRFRGIQSCGGK-GDIKEYELCGNPREHL 326
           +  D +   +  ++NP+KYG KE  +ACCG+G  RGI +CGG+ G  + YELC    ++L
Sbjct: 280 ALHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVTDYL 339

Query: 327 FYDANHGSDRGYQILAQMIWNGDSNSSRPVNVKSLFQ 359
           F+D  H +++ +Q +A++IW+G +N ++P N+++LF+
Sbjct: 340 FFDHFHLTEKAHQQIAELIWSGPTNVTKPYNLQALFE 374

BLAST of Sed0004039 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-78
Identity = 155/338 (45.86%), Postives = 216/338 (63.91%), Query Frame = 0

Query: 27  MFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQNFFKYPTGRWSDARIVPDFFAEYANLRL 86
           +F+FGDS  D GNNNYI T    QAN+ PYGQ FF  PTGR+SD R++ DF AEYANL L
Sbjct: 49  LFLFGDSFLDAGNNNYINTTTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPL 108

Query: 87  VLPYLHPWN-QHYMDGANFASGGAGALDQINRGFVISLNTQLRNFNKVERILRKQLGYSQ 146
           + P+L P N Q  + G NFAS GAGAL +  +G VI+L TQL ++ KVER+ R   G  +
Sbjct: 109 IPPFLEPGNSQKKLYGVNFASAGAGALVETFQGSVINLRTQLDHYKKVERLWRTNFGKEE 168

Query: 147 AKALLSKGVYLFSIGTNDYRT-FAQDPNLFNSYSLQEYVDWVIGNLTTVIQEIHKKGGRK 206
           +K  +S+ VYL SIG+NDY + F  + +L    S+ ++VD VIGNLTT I EI+K GGRK
Sbjct: 169 SKKRISRAVYLISIGSNDYSSIFLTNQSL--PISMSQHVDIVIGNLTTFIHEIYKIGGRK 228

Query: 207 FVMLNIWSPNHVPAVQEEVASRGKDARPEVLNYLIELHNRQLYGALQKLTTRLKGFRYSY 266
           F  LN+      PA++  +  +  D+     + L  +HNR L   L ++  ++KGF++S 
Sbjct: 229 FGFLNVPDLGCFPALR-ILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFSL 288

Query: 267 ADSYKVFNEMVSNPAKYGLKEVNVACCGSGRFRGIQSCGGKGDIKEYELCGNPREHLFYD 326
            D  K     + +P+K+G KE   ACCG+G++RG+ SCGGK  +KEY+LC NP++++F+D
Sbjct: 289 FDMNKSLRLRMQHPSKFGFKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWD 348

Query: 327 ANHGSDRGYQILAQMIWNG----DSNSSRPVNVKSLFQ 359
           + H +   Y   A +IWNG    DS    P N+ +LFQ
Sbjct: 349 SLHLTQNTYNQFANLIWNGGHMSDSLVVGPYNINNLFQ 383

BLAST of Sed0004039 vs. ExPASy Swiss-Prot
Match: Q9SYF5 (GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2)

HSP 1 Score: 284.6 bits (727), Expect = 1.5e-75
Identity = 150/340 (44.12%), Postives = 216/340 (63.53%), Query Frame = 0

Query: 27  MFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQNFFKYPTGRWSDARIVPDFFAEYANLRL 86
           +FVFGDS+FD GNNNYI T  + ++N WPYGQ  FK+PTGR SD         E A L  
Sbjct: 36  LFVFGDSLFDAGNNNYINTVSSFRSNIWPYGQTNFKFPTGRLSDG-------PEKAWLPS 95

Query: 87  VLPYLHP--WNQHYMDGANFASGGAGALDQINRGFVISLNTQLRNFNKVERILRKQLGYS 146
           + P L P   N  +  G +FAS GAGAL +   G VI+L TQL NF  VE+ LR +LG +
Sbjct: 96  IPPNLQPNNGNNQFTYGVSFASAGAGALAESFLGMVINLGTQLNNFKDVEKSLRSELGDA 155

Query: 147 QAKALLSKGVYLFSIGTNDY-RTFAQDPNLFNSYSLQEYVDWVIGNLTTVIQEIHKKGGR 206
           + K + S+ VYLF IG NDY   F+ + + F S S +++VD+VIGN+T VI+E++K GGR
Sbjct: 156 ETKRVFSRAVYLFHIGANDYFYPFSANSSTFKSNSKEKFVDFVIGNITFVIEEVYKMGGR 215

Query: 207 KFVMLNI----WSPNHVPAVQEEVASRGKDARPEVLNYLIELHNRQLYGALQKLTTRLKG 266
           KF  LN+     SPN +   + ++ S  K         LI++HN++    L++L  +L G
Sbjct: 216 KFGFLNVGPYECSPNSLIRDRTKIGSCFKPVAE-----LIDMHNKKFPDVLRRLQRQLSG 275

Query: 267 FRYSYADSYKVFNEMVSNPAKYGLKEVNVACCGSGRFRGIQSCGGK-GDIKEYELCGNPR 326
           FRY+  D +   +E +++P+KYG KE   ACCGSG  RGI +CG + G  + Y LC N  
Sbjct: 276 FRYALHDYHTSLSERINSPSKYGFKEGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVT 335

Query: 327 EHLFYDANHGSDRGYQILAQMIWNGDSNSSRPVNVKSLFQ 359
           ++LFYD++H +++ ++ +A++IWNG  N +RP N+K+LF+
Sbjct: 336 DYLFYDSSHLTEKAHRQIAELIWNGPPNVTRPYNLKALFE 363

BLAST of Sed0004039 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 3.1e-73
Identity = 152/353 (43.06%), Postives = 213/353 (60.34%), Query Frame = 0

Query: 6   ILCLCLFISFAAVGSACDPFPMFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQNFFKYPT 65
           +  LCL +    + S      +F+FGDS+FD GNNN+I T+   +AN+WPYGQ++F  PT
Sbjct: 14  VAVLCLSLPTGCLSSQ-QAAALFIFGDSVFDPGNNNHINTHVNFKANFWPYGQSYFSSPT 73

Query: 66  GRWSDARIVPDFFAEYANLRLVLPYLHPWNQHYMDGANFASGGAGALDQINRGFVISLNT 125
           GR+SD RI+PDF AEYA+L ++  YL P N  +  GANFAS GAGAL   + G  + L T
Sbjct: 74  GRFSDGRIIPDFIAEYASLPIIPAYLEP-NNDFTHGANFASAGAGALIASHAGLAVGLQT 133

Query: 126 QLRNFNKVERILRKQLGYSQAKALLSKGVYLFSIGTNDYRTFAQDPNLFNSYSLQEYVDW 185
           QLR F  +    R+ LG  +++ LLS  VYLFS G NDY    Q P  +  Y+ ++YVD 
Sbjct: 134 QLRYFGDLVDHYRQNLGDIKSRQLLSDAVYLFSCGGNDY----QSP--YYPYTQEQYVDI 193

Query: 186 VIGNLTTVIQEIHKKGGRKFVMLNIWSPNHVPAVQEEVASRGKDARPEVLNYLIELHNRQ 245
           VIGN+T VI+ I++KGGRKF ++N+      P ++ +    G     EV + L  LHN+ 
Sbjct: 194 VIGNMTNVIKGIYEKGGRKFGVVNVPLIGCWPGMRAK--QPGNTCNTEV-DELTRLHNQA 253

Query: 246 LYGALQKLTTRLKGFRYSYADSYKVFNEMVSNPAKYGLKEVNVACCGSGRFRGIQSCGGK 305
               L++L  +L+GF Y+  D        + NP+KYG KE   ACCGSG F G   C   
Sbjct: 254 FAKRLEQLEKQLEGFVYAKFDLSTAILNRMKNPSKYGFKEGESACCGSGPFGGNYDC--- 313

Query: 306 GDIKEYELCGNPREHLFYDANHGSDRGYQILAQMIWNGDSNSSRPVNVKSLFQ 359
           G IKE+ LC N  E+ F+D  H ++   +  A+M W+GDS  ++P N+K+LF+
Sbjct: 314 GRIKEFGLCDNATEYFFFDPFHPNELASRQFAEMFWDGDSMVTQPYNLKALFE 352

BLAST of Sed0004039 vs. ExPASy TrEMBL
Match: A0A5D3D6F7 (GDSL esterase/lipase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00630 PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 2.4e-145
Identity = 244/334 (73.05%), Postives = 290/334 (86.83%), Query Frame = 0

Query: 26  PMFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQNFFKYPTGRWSDARIVPDFFAEYANLR 85
           P+FVFGDSI D GNNNYI T P A++NY PYG  FFK P+GRWSD R+VPDFFA+YANL+
Sbjct: 12  PLFVFGDSIVDIGNNNYINTIPIARSNYPPYGLTFFKCPSGRWSDGRVVPDFFAQYANLQ 71

Query: 86  LVLPYLHPWNQHYMDGANFASGGAGALDQINRGFVISLNTQLRNFNKVERILRKQLGYSQ 145
           L+LPYL+P N+HY++G NFASGGAGALD+INRG VISL TQ R+F KVE+ILRKQ+G +Q
Sbjct: 72  LLLPYLYPGNKHYINGINFASGGAGALDEINRGLVISLKTQARSFKKVEKILRKQIGKTQ 131

Query: 146 AKALLSKGVYLFSIGTNDYRTFAQDPNLFNSYSLQEYVDWVIGNLTTVIQEIHKKGGRKF 205
           AKALLS+ VYL SIGTNDYRTFA +  LF+SYS +EYVD VIGNLT+VI+EI+K GGRKF
Sbjct: 132 AKALLSRAVYLISIGTNDYRTFASNSKLFDSYSFEEYVDLVIGNLTSVIKEIYKNGGRKF 191

Query: 206 VMLNIWSPNHVPAVQEEVASRGKDARPEVLNYLIELHNRQLYGALQKLTTRLKGFRYSYA 265
           V++N+WS NHVPAV E VAS+GKDA+ E LN L+E+HN+QLY A+QKLTT L+GFRYSY 
Sbjct: 192 VVMNLWSFNHVPAVLEAVASQGKDAQLEQLNQLVEMHNKQLYKAVQKLTTELQGFRYSYV 251

Query: 266 DSYKVFNEMVSNPAKYGLKEVNVACCGSGRFRGIQSCGGKGDIKEYELCGNPREHLFYDA 325
           DSYKVF E+ +NPAK+GLKEV  ACCGSG +RGIQSCGGKG++KEYELCGNP+EHLF+D+
Sbjct: 252 DSYKVFEEITNNPAKHGLKEVKNACCGSGVYRGIQSCGGKGNVKEYELCGNPKEHLFFDS 311

Query: 326 NHGSDRGYQILAQMIWNGDSNSSRPVNVKSLFQS 360
           NHGSD+ YQILA+M WNGD N+S PVNVKSLF S
Sbjct: 312 NHGSDKAYQILAEMAWNGDLNASTPVNVKSLFLS 345

BLAST of Sed0004039 vs. ExPASy TrEMBL
Match: A0A1S3CBT3 (GDSL esterase/lipase 1-like OS=Cucumis melo OX=3656 GN=LOC103499191 PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 2.4e-145
Identity = 244/334 (73.05%), Postives = 290/334 (86.83%), Query Frame = 0

Query: 26  PMFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQNFFKYPTGRWSDARIVPDFFAEYANLR 85
           P+FVFGDSI D GNNNYI T P A++NY PYG  FFK P+GRWSD R+VPDFFA+YANL+
Sbjct: 37  PLFVFGDSIVDIGNNNYINTIPIARSNYPPYGLTFFKCPSGRWSDGRVVPDFFAQYANLQ 96

Query: 86  LVLPYLHPWNQHYMDGANFASGGAGALDQINRGFVISLNTQLRNFNKVERILRKQLGYSQ 145
           L+LPYL+P N+HY++G NFASGGAGALD+INRG VISL TQ R+F KVE+ILRKQ+G +Q
Sbjct: 97  LLLPYLYPGNKHYINGINFASGGAGALDEINRGLVISLKTQARSFKKVEKILRKQIGKTQ 156

Query: 146 AKALLSKGVYLFSIGTNDYRTFAQDPNLFNSYSLQEYVDWVIGNLTTVIQEIHKKGGRKF 205
           AKALLS+ VYL SIGTNDYRTFA +  LF+SYS +EYVD VIGNLT+VI+EI+K GGRKF
Sbjct: 157 AKALLSRAVYLISIGTNDYRTFASNSKLFDSYSFEEYVDLVIGNLTSVIKEIYKNGGRKF 216

Query: 206 VMLNIWSPNHVPAVQEEVASRGKDARPEVLNYLIELHNRQLYGALQKLTTRLKGFRYSYA 265
           V++N+WS NHVPAV E VAS+GKDA+ E LN L+E+HN+QLY A+QKLTT L+GFRYSY 
Sbjct: 217 VVMNLWSFNHVPAVLEAVASQGKDAQLEQLNQLVEMHNKQLYKAVQKLTTELQGFRYSYV 276

Query: 266 DSYKVFNEMVSNPAKYGLKEVNVACCGSGRFRGIQSCGGKGDIKEYELCGNPREHLFYDA 325
           DSYKVF E+ +NPAK+GLKEV  ACCGSG +RGIQSCGGKG++KEYELCGNP+EHLF+D+
Sbjct: 277 DSYKVFEEITNNPAKHGLKEVKNACCGSGVYRGIQSCGGKGNVKEYELCGNPKEHLFFDS 336

Query: 326 NHGSDRGYQILAQMIWNGDSNSSRPVNVKSLFQS 360
           NHGSD+ YQILA+M WNGD N+S PVNVKSLF S
Sbjct: 337 NHGSDKAYQILAEMAWNGDLNASTPVNVKSLFLS 370

BLAST of Sed0004039 vs. ExPASy TrEMBL
Match: A0A6J1IZW0 (GDSL esterase/lipase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111479956 PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 9.3e-145
Identity = 250/368 (67.93%), Postives = 297/368 (80.71%), Query Frame = 0

Query: 1   MANSSILCLCLFISFAAVGSAC--------DPFPMFVFGDSIFDTGNNNYIRTNPTAQAN 60
           MA+S+ L LC+   F AVG  C           P+F FGDS+ DTGNNNYI T   AQAN
Sbjct: 1   MASSTNLRLCVLFIFFAVGCVCMQDEQLHNHHGPLFTFGDSLVDTGNNNYINTRRIAQAN 60

Query: 61  YWPYGQNFFKYPTGRWSDARIVPDFFAEYANLRLVLPYLHPWNQHYMDGANFASGGAGAL 120
           Y PYGQ FFKYP+GRWSD R++PDFFAEYA+L  +LPYLHP N+ Y+ G NFASGGAGAL
Sbjct: 61  YPPYGQTFFKYPSGRWSDGRVLPDFFAEYADLPWILPYLHPGNKRYVYGTNFASGGAGAL 120

Query: 121 DQINRGFVISLNTQLRNFNKVERILRKQLGYSQAKALLSKGVYLFSIGTNDYRTFAQDPN 180
            + N+G V+SL TQ RNF KVERILRKQLG +    +LSKGVYL  IGTNDY  +A D  
Sbjct: 121 HETNQGLVMSLKTQARNFKKVERILRKQLGRTGVNTILSKGVYLIWIGTNDYSVYASDSK 180

Query: 181 LFNSYSLQEYVDWVIGNLTTVIQEIHKKGGRKFVMLNIWSPNHVPAVQEE-VASRGKDAR 240
           LF+SYSL++YVD VIGNL++VI+EIHKKGGRKFV++N+WSPNH+P VQEE VAS+G+DAR
Sbjct: 181 LFSSYSLEKYVDLVIGNLSSVIEEIHKKGGRKFVVINLWSPNHIPLVQEEAVASQGRDAR 240

Query: 241 PEVLNYLIELHNRQLYGALQKLTTRLKGFRYSYADSYKVFNEMVSNPAKYGLKEVNVACC 300
              LN+L+ELHN QLY  LQ+LTT+LKGFRYSYADSYKV  E+ SNPAKYG K+V VACC
Sbjct: 241 LGQLNHLVELHNGQLYKELQRLTTKLKGFRYSYADSYKVVEEIASNPAKYGFKDVKVACC 300

Query: 301 GSGRFRGIQSCGGKGDIKEYELCGNPREHLFYDANHGSDRGYQILAQMIWNGDSNSSRPV 360
           GSG+ RGIQSCGG+  +KEY+LC NP+EHLF+D+NHGSD+GYQILA+MIWNGD N+SRP+
Sbjct: 301 GSGKLRGIQSCGGQDTLKEYQLCENPKEHLFFDSNHGSDKGYQILAEMIWNGDLNTSRPI 360

BLAST of Sed0004039 vs. ExPASy TrEMBL
Match: A0A6J1DI34 (GDSL esterase/lipase 1-like OS=Momordica charantia OX=3673 GN=LOC111020259 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.5e-142
Identity = 252/351 (71.79%), Postives = 285/351 (81.20%), Query Frame = 0

Query: 15  FAAVGSACD---PFPMFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQNFFKYPTGRWSDA 74
           F+  GS CD     P+FV GDS  DTGNNNYI T P AQ+NY PYGQ FFK+PTGRWSD 
Sbjct: 5   FSGAGSVCDHDQHVPLFVLGDSFVDTGNNNYINTIPVAQSNYSPYGQTFFKFPTGRWSDG 64

Query: 75  RIVPDFFAEYANLRLVLPYLHPWNQH-YMDGANFASGGAGALDQINRGFVISLNTQLRNF 134
           R++PDF AEYANL L+LPYLHP N H Y  G NFASGGAGAL Q ++G VISL TQL  F
Sbjct: 65  RVLPDFLAEYANLPLLLPYLHPGNNHFYTHGINFASGGAGALHQTHQGLVISLKTQLSYF 124

Query: 135 NKVERILRKQLGYS-QAKALLSKGVYLFSIGTNDYRTFAQDPNLFNSYSLQEYVDWVIGN 194
           NKV+RILRKQLG S QA+ LLS+GVYL SIG NDY  FA D  L +SYSL++YVD VIGN
Sbjct: 125 NKVKRILRKQLGGSTQAETLLSRGVYLISIGANDYGAFASDSKLLHSYSLEQYVDMVIGN 184

Query: 195 LTTVIQEIHKKGGRKFVMLNIWSPNHVPAVQEEVASR-GKDARPEVLNYLIELHNRQLYG 254
           LT+ I EIHK+GGRKFV+LN+W  NHVPAV+E VAS+ GKD+R E L  L+ELHNRQLY 
Sbjct: 185 LTSAITEIHKRGGRKFVVLNVWPLNHVPAVEEAVASQAGKDSRVEQLTQLVELHNRQLYK 244

Query: 255 ALQKLTTRLKGFRYSYADSYKVFNEMVSNPAKYGLKEVNVACCGSGRFRGIQSCGGKGDI 314
           ALQKL+T L GF YSYAD Y V N ++SNPAKYG+KEV  ACCGSG+FRGIQSCGGKGD 
Sbjct: 245 ALQKLSTELDGFIYSYADFYNVINGIISNPAKYGIKEVKAACCGSGKFRGIQSCGGKGDA 304

Query: 315 KEYELCGNPREHLFYDANHGSDRGYQILAQMIWNGDSNSSRPVNVKSLFQS 360
           KEYELCGNP E+LF+D+NHGSDRGY+ILA+MIWNGDS SSRPVNVKSLFQS
Sbjct: 305 KEYELCGNPNEYLFFDSNHGSDRGYKILAEMIWNGDSESSRPVNVKSLFQS 355

BLAST of Sed0004039 vs. ExPASy TrEMBL
Match: A0A6J1GX03 (GDSL esterase/lipase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111457919 PE=3 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 5.6e-142
Identity = 245/368 (66.58%), Postives = 295/368 (80.16%), Query Frame = 0

Query: 1   MANSSILCLCLFISFAAVGSAC--------DPFPMFVFGDSIFDTGNNNYIRTNPTAQAN 60
           MA+S+ L LC+   F AVG  C           P+F FGDS+ DTGNNNYI T   AQAN
Sbjct: 1   MASSTNLRLCVLFIFFAVGCVCMQDEQLHIHHVPLFTFGDSLADTGNNNYINTRRIAQAN 60

Query: 61  YWPYGQNFFKYPTGRWSDARIVPDFFAEYANLRLVLPYLHPWNQHYMDGANFASGGAGAL 120
           Y PYGQ FFKYP+GRWSD R++PDFFAEYA+L  +LPYLHP N+ Y+ G NFASGGAGAL
Sbjct: 61  YPPYGQTFFKYPSGRWSDGRVLPDFFAEYADLPWLLPYLHPGNKRYVYGTNFASGGAGAL 120

Query: 121 DQINRGFVISLNTQLRNFNKVERILRKQLGYSQAKALLSKGVYLFSIGTNDYRTFAQDPN 180
            + N+G VISL TQ+RNF KVERILRKQLG +    +LSKGVYL  IGTNDY  +  D  
Sbjct: 121 CETNQGLVISLKTQVRNFKKVERILRKQLGKTGVNTILSKGVYLIWIGTNDYAVYTSDSK 180

Query: 181 LFNSYSLQEYVDWVIGNLTTVIQEIHKKGGRKFVMLNIWSPNHVPAVQEE-VASRGKDAR 240
           LF+SYSL++YVD VIGNLT+VI+EI+KKGGRKFV++N+WSPNH+P VQEE VAS+G+DAR
Sbjct: 181 LFSSYSLEKYVDMVIGNLTSVIKEIYKKGGRKFVVMNLWSPNHIPLVQEEAVASQGRDAR 240

Query: 241 PEVLNYLIELHNRQLYGALQKLTTRLKGFRYSYADSYKVFNEMVSNPAKYGLKEVNVACC 300
              LN+L++LHN QLY  LQ+LTT+LKGF+YS+ADSYKV  E+ SNPAKYG K+V VACC
Sbjct: 241 LGQLNHLVDLHNGQLYKELQRLTTKLKGFKYSHADSYKVVEEIASNPAKYGFKDVKVACC 300

Query: 301 GSGRFRGIQSCGGKGDIKEYELCGNPREHLFYDANHGSDRGYQILAQMIWNGDSNSSRPV 360
           GSG+ RGIQSCGG+  +KEY+LC NP+EHLF+D+NHGSD+GYQ LA+MIWNGD N+SRP+
Sbjct: 301 GSGKLRGIQSCGGQDTVKEYQLCENPKEHLFFDSNHGSDKGYQTLAEMIWNGDLNTSRPI 360

BLAST of Sed0004039 vs. TAIR 10
Match: AT5G40990.1 (GDSL lipase 1 )

HSP 1 Score: 310.8 bits (795), Expect = 1.4e-84
Identity = 157/336 (46.73%), Postives = 222/336 (66.07%), Query Frame = 0

Query: 27  MFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQNFFKYPTGRWSDARIVPDFFAEYANLRL 86
           +FVFGDS+FD GNNNYI T  + ++NYWPYGQ  FK PTGR SD R++PDF AEYA L L
Sbjct: 38  LFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLPL 97

Query: 87  VLPYLHPW--NQHYMDGANFASGGAGALDQINRGFVISLNTQLRNFNKVERILRKQLGYS 146
           + P L P+  N  +  G NFASGGAGAL     G VI+L TQL NF KVE +LR +LG +
Sbjct: 98  IPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLVINLRTQLNNFKKVEEMLRSKLGDA 157

Query: 147 QAKALLSKGVYLFSIGTNDYR-TFAQDPNLFNSYSLQEYVDWVIGNLTTVIQEIHKKGGR 206
           + K ++S+ VYLF IG NDY+  F  + +LF S S ++YVD+V+GN+T V +E++  GGR
Sbjct: 158 EGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSISNEKYVDYVVGNMTDVFKEVYNLGGR 217

Query: 207 KFVMLNIWSPNHVPAVQEEVASRGKDARPEVLNYLIELHNRQLYGALQKLTTRLKGFRYS 266
           KF +LN    +  PA      ++ +     V   LI +HN +L   L++L   L GF+Y+
Sbjct: 218 KFGILNTGPYDCAPASLVIDQTKIRSCFQPVTE-LINMHNEKLLNGLRRLNHELSGFKYA 277

Query: 267 YADSYKVFNEMVSNPAKYGLKEVNVACCGSGRFRGIQSCGGK-GDIKEYELCGNPREHLF 326
             D +   +E +++P+KYG KE   ACCGSG  RGI +CGG+ G  + YELC N  ++LF
Sbjct: 278 LHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLF 337

Query: 327 YDANHGSDRGYQILAQMIWNGDSNSSRPVNVKSLFQ 359
           +D  H +++  + +A++IW+G +N + P N+K+LF+
Sbjct: 338 FDPFHLTEKANRQIAELIWSGPTNITGPYNLKALFE 372

BLAST of Sed0004039 vs. TAIR 10
Match: AT1G53940.1 (GDSL-motif lipase 2 )

HSP 1 Score: 298.5 bits (763), Expect = 7.1e-81
Identity = 153/328 (46.65%), Postives = 216/328 (65.85%), Query Frame = 0

Query: 27  MFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQNFFKYPTGRWSDARIVPDFFAEYANLRL 86
           +FVFGDS+FD GNNNYI T P+ ++NYWPYGQ  FK+PTGR SD R +PDF AEYA L L
Sbjct: 40  LFVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPL 99

Query: 87  VLPYLHPWN--QHYMDGANFASGGAGALDQINRGFVISLNTQLRNFNKVERILRKQLGYS 146
           +  YL P N    +  G +FAS GAGAL     G VI+L +QL NF KVE++LR  LG +
Sbjct: 100 IPAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEA 159

Query: 147 QAKALLSKGVYLFSIGTNDYR-TFAQDPNLFNSYSLQEYVDWVIGNLTTVIQEIHKKGGR 206
           Q K ++S+ VYLF IG NDY+  F+ + ++F S   + YVD+V+GN T VI+E++K GGR
Sbjct: 160 QGKMVISRAVYLFHIGVNDYQYPFSTNSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGGR 219

Query: 207 KFVMLNIWSPNHVPA-VQEEVASRGKDARPEVLNYLIELHNRQLYGALQKLTTRLKGFRY 266
           KF  LN+ + +  PA +  +    G   +P  +  LI LHN +L   L++L   L GF+Y
Sbjct: 220 KFGFLNMGAYDCAPASLIIDQTKIGTCFKP--VTELINLHNEKLESGLRRLERELSGFKY 279

Query: 267 SYADSYKVFNEMVSNPAKYGLKEVNVACCGSGRFRGIQSCGGK-GDIKEYELCGNPREHL 326
           +  D +   +  ++NP+KYG KE  +ACCG+G  RGI +CGG+ G  + YELC    ++L
Sbjct: 280 ALHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVTDYL 339

Query: 327 FYDANHGSDRGYQILAQMIWNGDSNSSR 350
           F+D  H +++ +Q +A++IW+G +N  R
Sbjct: 340 FFDHFHLTEKAHQQIAELIWSGPTNGLR 365

BLAST of Sed0004039 vs. TAIR 10
Match: AT1G53920.1 (GDSL-motif lipase 5 )

HSP 1 Score: 295.0 bits (754), Expect = 7.9e-80
Identity = 155/338 (45.86%), Postives = 216/338 (63.91%), Query Frame = 0

Query: 27  MFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQNFFKYPTGRWSDARIVPDFFAEYANLRL 86
           +F+FGDS  D GNNNYI T    QAN+ PYGQ FF  PTGR+SD R++ DF AEYANL L
Sbjct: 49  LFLFGDSFLDAGNNNYINTTTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPL 108

Query: 87  VLPYLHPWN-QHYMDGANFASGGAGALDQINRGFVISLNTQLRNFNKVERILRKQLGYSQ 146
           + P+L P N Q  + G NFAS GAGAL +  +G VI+L TQL ++ KVER+ R   G  +
Sbjct: 109 IPPFLEPGNSQKKLYGVNFASAGAGALVETFQGSVINLRTQLDHYKKVERLWRTNFGKEE 168

Query: 147 AKALLSKGVYLFSIGTNDYRT-FAQDPNLFNSYSLQEYVDWVIGNLTTVIQEIHKKGGRK 206
           +K  +S+ VYL SIG+NDY + F  + +L    S+ ++VD VIGNLTT I EI+K GGRK
Sbjct: 169 SKKRISRAVYLISIGSNDYSSIFLTNQSL--PISMSQHVDIVIGNLTTFIHEIYKIGGRK 228

Query: 207 FVMLNIWSPNHVPAVQEEVASRGKDARPEVLNYLIELHNRQLYGALQKLTTRLKGFRYSY 266
           F  LN+      PA++  +  +  D+     + L  +HNR L   L ++  ++KGF++S 
Sbjct: 229 FGFLNVPDLGCFPALR-ILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFSL 288

Query: 267 ADSYKVFNEMVSNPAKYGLKEVNVACCGSGRFRGIQSCGGKGDIKEYELCGNPREHLFYD 326
            D  K     + +P+K+G KE   ACCG+G++RG+ SCGGK  +KEY+LC NP++++F+D
Sbjct: 289 FDMNKSLRLRMQHPSKFGFKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWD 348

Query: 327 ANHGSDRGYQILAQMIWNG----DSNSSRPVNVKSLFQ 359
           + H +   Y   A +IWNG    DS    P N+ +LFQ
Sbjct: 349 SLHLTQNTYNQFANLIWNGGHMSDSLVVGPYNINNLFQ 383

BLAST of Sed0004039 vs. TAIR 10
Match: AT1G53990.1 (GDSL-motif lipase 3 )

HSP 1 Score: 284.6 bits (727), Expect = 1.1e-76
Identity = 150/340 (44.12%), Postives = 216/340 (63.53%), Query Frame = 0

Query: 27  MFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQNFFKYPTGRWSDARIVPDFFAEYANLRL 86
           +FVFGDS+FD GNNNYI T  + ++N WPYGQ  FK+PTGR SD         E A L  
Sbjct: 36  LFVFGDSLFDAGNNNYINTVSSFRSNIWPYGQTNFKFPTGRLSDG-------PEKAWLPS 95

Query: 87  VLPYLHP--WNQHYMDGANFASGGAGALDQINRGFVISLNTQLRNFNKVERILRKQLGYS 146
           + P L P   N  +  G +FAS GAGAL +   G VI+L TQL NF  VE+ LR +LG +
Sbjct: 96  IPPNLQPNNGNNQFTYGVSFASAGAGALAESFLGMVINLGTQLNNFKDVEKSLRSELGDA 155

Query: 147 QAKALLSKGVYLFSIGTNDY-RTFAQDPNLFNSYSLQEYVDWVIGNLTTVIQEIHKKGGR 206
           + K + S+ VYLF IG NDY   F+ + + F S S +++VD+VIGN+T VI+E++K GGR
Sbjct: 156 ETKRVFSRAVYLFHIGANDYFYPFSANSSTFKSNSKEKFVDFVIGNITFVIEEVYKMGGR 215

Query: 207 KFVMLNI----WSPNHVPAVQEEVASRGKDARPEVLNYLIELHNRQLYGALQKLTTRLKG 266
           KF  LN+     SPN +   + ++ S  K         LI++HN++    L++L  +L G
Sbjct: 216 KFGFLNVGPYECSPNSLIRDRTKIGSCFKPVAE-----LIDMHNKKFPDVLRRLQRQLSG 275

Query: 267 FRYSYADSYKVFNEMVSNPAKYGLKEVNVACCGSGRFRGIQSCGGK-GDIKEYELCGNPR 326
           FRY+  D +   +E +++P+KYG KE   ACCGSG  RGI +CG + G  + Y LC N  
Sbjct: 276 FRYALHDYHTSLSERINSPSKYGFKEGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVT 335

Query: 327 EHLFYDANHGSDRGYQILAQMIWNGDSNSSRPVNVKSLFQ 359
           ++LFYD++H +++ ++ +A++IWNG  N +RP N+K+LF+
Sbjct: 336 DYLFYDSSHLTEKAHRQIAELIWNGPPNVTRPYNLKALFE 363

BLAST of Sed0004039 vs. TAIR 10
Match: AT3G14225.1 (GDSL-motif lipase 4 )

HSP 1 Score: 269.2 bits (687), Expect = 4.6e-72
Identity = 147/367 (40.05%), Postives = 225/367 (61.31%), Query Frame = 0

Query: 6   ILCLCLFISFAAVGSAC------DPFPMFVFGDSIFDTGNNNYIRTNPTAQANYWPYGQN 65
           IL +C  IS + V  +C      +   +F FGDS+F+ GNNNY  +  + ++N+WPYG+ 
Sbjct: 12  ILFICT-ISLSIVSISCKEDLKTNQAALFAFGDSLFEAGNNNYFDSISSFRSNFWPYGKT 71

Query: 66  FFKYPTGRWSDARIVPDFFAEYANLRLVLPYLHPW--NQHYMDGANFASGGAGALDQINR 125
            FK+PTGR SD RI+ DF AEYA L L+ P L P   N     G NFA+  AG       
Sbjct: 72  TFKFPTGRVSDGRIMIDFIAEYAWLPLIPPNLQPGYSNSQLTYGLNFATTAAGVFAGTFP 131

Query: 126 GFVISLN----TQLRNFNKVERILRKQLGYSQAKALLSKGVYLFSIGTNDYR-TFAQDPN 185
           G V +L+    TQL NF  VE+ LR  LG ++A+ ++SK VYLF IG NDY+  F  + +
Sbjct: 132 GSVTNLSKDLGTQLNNFKNVEKTLRSNLGDAEARRVISKAVYLFHIGANDYQYPFFANTS 191

Query: 186 LFNSYSLQEYVDWVIGNLTTVIQEIHKKGGRKFVMLNIWSPNHVPAVQEEVASRGKDARP 245
            F++ + + ++D+VIGN TTVI+E++K G RKF  L++      P+    + S    +  
Sbjct: 192 TFSNTTKERFIDFVIGNTTTVIEELYKLGARKFGFLSLGPFGCTPSAL-IINSTKIGSCF 251

Query: 246 EVLNYLIELHNRQLYGALQKLTTRLKGFRYSYADSYKVFNEMVSNPAKYGLKEVNVACCG 305
           E +  LI LHN++    L++L  RL GF+Y+  D +   ++ ++NP++YG KE  +ACCG
Sbjct: 252 EPVTELINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRYGFKEGEMACCG 311

Query: 306 SGRFRGIQSCGGK-GDIKEYELCGNPREHLFYDANHGSDRGYQILAQMIWNGDSNSSRPV 359
           SG  RGI +CG + G  + Y+LC N  +++F+D +H ++  +Q +A++IW+G  N + P 
Sbjct: 312 SGPLRGINTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAELIWSGPPNVTAPY 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903481.18.9e-15070.89GDSL esterase/lipase 1-like [Benincasa hispida][more]
XP_004144442.11.7e-14870.57GDSL esterase/lipase 1 [Cucumis sativus] >KAE8650888.1 hypothetical protein Csa_... [more]
XP_008460337.15.0e-14573.05PREDICTED: GDSL esterase/lipase 1-like [Cucumis melo][more]
KAA0053544.15.0e-14573.05GDSL esterase/lipase 1-like [Cucumis melo var. makuwa] >TYK19139.1 GDSL esterase... [more]
XP_022980654.11.9e-14467.93GDSL esterase/lipase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FLN02.0e-8346.73GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1[more]
Q9SYF09.7e-8346.29GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1[more]
Q9SSA71.1e-7845.86GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2[more]
Q9SYF51.5e-7544.12GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2[more]
H6U1I83.1e-7343.06GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3D6F72.4e-14573.05GDSL esterase/lipase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3CBT32.4e-14573.05GDSL esterase/lipase 1-like OS=Cucumis melo OX=3656 GN=LOC103499191 PE=3 SV=1[more]
A0A6J1IZW09.3e-14567.93GDSL esterase/lipase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111479956 PE=3 SV=... [more]
A0A6J1DI341.5e-14271.79GDSL esterase/lipase 1-like OS=Momordica charantia OX=3673 GN=LOC111020259 PE=3 ... [more]
A0A6J1GX035.6e-14266.58GDSL esterase/lipase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111457919 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT5G40990.11.4e-8446.73GDSL lipase 1 [more]
AT1G53940.17.1e-8146.65GDSL-motif lipase 2 [more]
AT1G53920.17.9e-8045.86GDSL-motif lipase 5 [more]
AT1G53990.11.1e-7644.12GDSL-motif lipase 3 [more]
AT3G14225.14.6e-7240.05GDSL-motif lipase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 25..345
e-value: 3.7E-65
score: 222.4
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 28..338
e-value: 4.4E-20
score: 73.1
IPR044552GDSL esterase/lipase GLIP1-5/GLL25PANTHERPTHR45966GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 6..358
NoneNo IPR availablePANTHERPTHR45966:SF18GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 6..358
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 26..342
IPR008265Lipase, GDSL, active sitePROSITEPS01098LIPASE_GDSL_SERcoord: 27..38
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 27..344
e-value: 1.8655E-106
score: 313.013

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0004039.1Sed0004039.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0016298 lipase activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds