Sed0003159 (gene) Chayote v1

BLAST of Sed0003159 vs. NCBI nr
Match: KAG6601573.1 (Stemmadenine O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 643.7 bits (1659), Expect = 1.2e-180
Identity = 314/441 (71.20%), Postives = 363/441 (82.31%), Query Frame = 0

Query: 1   MAFEMEMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFYSA--VTATKITD 60
           +A  +++++VSKE +KPS  TPDH RRY LSFLDQ+TVDVYNPM+YFYSA   TA +I+D
Sbjct: 35  IAMAVDIDVVSKETVKPSSPTPDHHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISD 94

Query: 61  QLKSSLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELNK 120
            LK SLS +LSHYYPLAGR  Y +L IDCNDAGVPF+E RV NCRL DV NT FPTELNK
Sbjct: 95  HLKESLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRV-NCRLSDVMNTAFPTELNK 154

Query: 121 LIPFQLDELDEVSMGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRGETGAP 180
           L+PF+LD LD+VSMGVQLNVFECGGVAVGICISHKI DALSF   VNGWAAVCRGE  A 
Sbjct: 155 LLPFELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVCRGEKEAL 214

Query: 181 RGLHLTSAEAFPPAKTPVYNTRISILRERAAKRYRIDGGNVEAIRSKYEERKGGENERRV 240
              HL+++E FPP KTP+YNTR SI R+R AKRY+ID  ++E IR++Y E    EN+RR 
Sbjct: 215 E-THLSASELFPPNKTPIYNTRTSIFRQRVAKRYQIDAASIETIRAQYAEYFAMENQRRP 274

Query: 241 SRLEALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLDPPLPEYAFG 300
           SR+EALSAFLYGRF+AAIK  SN    N S++SEKKI+ VCHAVNIR RLDPP+P+YAFG
Sbjct: 275 SRVEALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFG 334

Query: 301 NYYRSTFALPSTEILNDHYCYGLVNKTREEIAKIDKEYLRNLQESTKFLDIMKKTVAQFT 360
           NYYR+TFA+PS  ILND YCY LV   R+EIAKIDK YL  LQES+KFL+ MKK  +QF+
Sbjct: 335 NYYRATFAVPSETILNDDYCYDLVKLARDEIAKIDKSYLERLQESSKFLEAMKKAASQFS 394

Query: 361 SGELISCAFTSLCRMPLYDADFGWGKPVWIGSPALTFKNLFVLMDEKDGDGIDVYVHLEE 420
           +GELISC+FTSLCRMP+YDADFGWGKP WI SPAL FKNLFVLMD+KDGDG+DVYVHL+ 
Sbjct: 395 TGELISCSFTSLCRMPIYDADFGWGKPAWISSPALIFKNLFVLMDKKDGDGVDVYVHLKP 454

Query: 421 EHMMKLEVDEVFLKYAKLPSN 440
           E+M  LE DE FLKYAKLPSN
Sbjct: 455 ENMEILESDEKFLKYAKLPSN 473

BLAST of Sed0003159 vs. NCBI nr
Match: XP_038893435.1 (stemmadenine O-acetyltransferase-like [Benincasa hispida])

HSP 1 Score: 643.3 bits (1658), Expect = 1.6e-180
Identity = 315/437 (72.08%), Postives = 365/437 (83.52%), Query Frame = 0

Query: 5   MEMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFYSAVTAT--KITDQLKS 64
           +E++I+SKE IKPS  TP+HLRRY+L+FLDQ+TVDVYNPMVYFY+A +AT  +I + LK 
Sbjct: 3   VEIQIISKETIKPSSPTPEHLRRYSLAFLDQVTVDVYNPMVYFYTAGSATPAEIANHLKK 62

Query: 65  SLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELNKLIPF 124
           SLSD+L+HYYPLAGR  Y +  IDCND G+PF+E RV NCRL DV NTPFP+ELNK +PF
Sbjct: 63  SLSDVLTHYYPLAGRVNYDEFFIDCNDDGIPFIETRV-NCRLSDVMNTPFPSELNKFLPF 122

Query: 125 QLDELDEVSMGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRGETGAPRGLH 184
           +LDELDEVSMGVQLNVFECGGVAVGICISHKI DALSF   VNGWAA CRGE  A R  H
Sbjct: 123 ELDELDEVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAYCRGEKEALR-TH 182

Query: 185 LTSAEAFPPAKTPVYNTRISILRERAAKRYRIDGGNVEAIRSKYEERKGGENERRVSRLE 244
           L+SAE FPPAKT +YNTR SI R+R A+RY+ID  NVE+IR+KY E    +N+RR SR+E
Sbjct: 183 LSSAELFPPAKTGLYNTRTSIFRQRVARRYQIDAVNVESIRAKYAECPAMQNQRRASRVE 242

Query: 245 ALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLDPPLPEYAFGNYYR 304
           ALSAF+Y RF+AAIKAVS    E+ S++SEKKI+ VCHAVNIR RLDPP+PEYAFGNYYR
Sbjct: 243 ALSAFIYSRFIAAIKAVSM---EDRSSESEKKIFLVCHAVNIRSRLDPPVPEYAFGNYYR 302

Query: 305 STFALPSTEILNDHYCYGLVNKTREEIAKIDKEYLRNLQESTKFLDIMKKTVAQFTSGEL 364
           STFA+PS  ILND+YCY LV   REEI KIDK YL+ LQES+KFLD MKK  AQF++GEL
Sbjct: 303 STFAVPSEGILNDNYCYDLVKLAREEIGKIDKHYLQRLQESSKFLDSMKKAAAQFSTGEL 362

Query: 365 ISCAFTSLCRMPLYDADFGWGKPVWIGSPALTFKNLFVLMDEKDGDGIDVYVHLEEEHMM 424
           ISC+FTSLCRMP+YD DFGWGKP WI SPAL FKNLFV +D+KDGDG+D+YVHL+EEHM 
Sbjct: 363 ISCSFTSLCRMPIYDVDFGWGKPEWISSPALIFKNLFVFIDKKDGDGVDIYVHLKEEHMN 422

Query: 425 KLEVDEVFLKYAKLPSN 440
           K E DE FLKYAKLPSN
Sbjct: 423 KFEADEEFLKYAKLPSN 434

BLAST of Sed0003159 vs. NCBI nr
Match: XP_022997942.1 (vinorine synthase-like [Cucurbita maxima])

HSP 1 Score: 642.5 bits (1656), Expect = 2.7e-180
Identity = 314/437 (71.85%), Postives = 360/437 (82.38%), Query Frame = 0

Query: 5   MEMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFYSA--VTATKITDQLKS 64
           +++E+VSKE +KPS  TPDH RRY LSFLDQ+TVDVYNPM+YFYSA   TA +I+D LK 
Sbjct: 3   VDIEVVSKETVKPSSPTPDHHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAGEISDHLKK 62

Query: 65  SLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELNKLIPF 124
           SLS +LSHYYPLAGR  Y +L IDCNDAGVPF+E RV NCRL DV NT FPTELNKL+PF
Sbjct: 63  SLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRV-NCRLSDVMNTAFPTELNKLLPF 122

Query: 125 QLDELDEVSMGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRGETGAPRGLH 184
           +LD LD+VSMGVQLNVFECGGVAVGICISHKI DALSF   VNGWAAVCRGE  A    H
Sbjct: 123 ELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVCRGEKEALE-TH 182

Query: 185 LTSAEAFPPAKTPVYNTRISILRERAAKRYRIDGGNVEAIRSKYEERKGGENERRVSRLE 244
           L+S+E FPP+KTP+YNTR SI R+R AKRY+ID  ++E IR++Y +    EN+RR SR+E
Sbjct: 183 LSSSELFPPSKTPIYNTRTSIFRQRVAKRYQIDAASIETIRAQYADYFAMENQRRPSRVE 242

Query: 245 ALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLDPPLPEYAFGNYYR 304
           ALSAFLYGRF+AAIK  SN    N  ++SEKKI+ VCHAVNIR RLDPP+P+YAFGNYYR
Sbjct: 243 ALSAFLYGRFIAAIKGASNSNPANGISESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR 302

Query: 305 STFALPSTEILNDHYCYGLVNKTREEIAKIDKEYLRNLQESTKFLDIMKKTVAQFTSGEL 364
           +TFA+PS  ILND YCY LV   REEIAKIDK YL  LQES+KFL+ MKK  AQF++GEL
Sbjct: 303 ATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL 362

Query: 365 ISCAFTSLCRMPLYDADFGWGKPVWIGSPALTFKNLFVLMDEKDGDGIDVYVHLEEEHMM 424
           ISC+FTSLCRMP+YDADFGWGKP WI SPAL FKNLFVLMD+ DGDG+DVYVHL+ E+M 
Sbjct: 363 ISCSFTSLCRMPIYDADFGWGKPAWISSPALIFKNLFVLMDKNDGDGVDVYVHLKPENME 422

Query: 425 KLEVDEVFLKYAKLPSN 440
            LE DE FLKYAKLPSN
Sbjct: 423 ILEADEKFLKYAKLPSN 437

BLAST of Sed0003159 vs. NCBI nr
Match: XP_022956577.1 (vinorine synthase-like [Cucurbita moschata])

HSP 1 Score: 642.5 bits (1656), Expect = 2.7e-180
Identity = 314/437 (71.85%), Postives = 361/437 (82.61%), Query Frame = 0

Query: 5   MEMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFYSA--VTATKITDQLKS 64
           +++E+VSKE +KPS  TPDH RRY LSFLDQ+TVDVYNPM+YFYSA   TA +I++ LK 
Sbjct: 3   VDIEVVSKETVKPSSPTPDHHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISNHLKE 62

Query: 65  SLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELNKLIPF 124
           SLS +LSHYYPLAGR  Y +L IDCNDAGVPF+E RV NCRL DV NT FPTELNKL+PF
Sbjct: 63  SLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRV-NCRLSDVMNTAFPTELNKLLPF 122

Query: 125 QLDELDEVSMGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRGETGAPRGLH 184
           +LD LD+VSMGVQLNVFECGGVAVGICISHKI DALSF   VNGWAAVCRGE  A    H
Sbjct: 123 ELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVCRGEKEALE-TH 182

Query: 185 LTSAEAFPPAKTPVYNTRISILRERAAKRYRIDGGNVEAIRSKYEERKGGENERRVSRLE 244
           L+++E FPP KTP+YNTR SI R+R AKRY+ID  ++E IR++Y E    EN+RR SR+E
Sbjct: 183 LSASELFPPNKTPIYNTRTSIFRQRVAKRYQIDAASIETIRAQYAEYFAMENQRRPSRVE 242

Query: 245 ALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLDPPLPEYAFGNYYR 304
           ALSAFLYGRF+AAIK  SN    N S++SEKKI+ VCHAVNIR RLDPP+P+YAFGNYYR
Sbjct: 243 ALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR 302

Query: 305 STFALPSTEILNDHYCYGLVNKTREEIAKIDKEYLRNLQESTKFLDIMKKTVAQFTSGEL 364
           +TFA+PS  ILND YCY LV   R+EIAKIDK YL  LQES+KFL+ MKK  AQF++GEL
Sbjct: 303 ATFAVPSETILNDDYCYDLVKLARDEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL 362

Query: 365 ISCAFTSLCRMPLYDADFGWGKPVWIGSPALTFKNLFVLMDEKDGDGIDVYVHLEEEHMM 424
           ISC+FTSLCRMP+YDADFGWGKP WI SPAL FKNLFVLMD+KDGDG+DVYVHL+ E+M 
Sbjct: 363 ISCSFTSLCRMPIYDADFGWGKPAWISSPALIFKNLFVLMDKKDGDGVDVYVHLKPENME 422

Query: 425 KLEVDEVFLKYAKLPSN 440
            LE DE FLKYAKLPSN
Sbjct: 423 ILESDEKFLKYAKLPSN 437

BLAST of Sed0003159 vs. NCBI nr
Match: KAG7032338.1 (Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 642.5 bits (1656), Expect = 2.7e-180
Identity = 315/443 (71.11%), Postives = 364/443 (82.17%), Query Frame = 0

Query: 1   MAFEMEMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFYSA--VTATKITD 60
           +A  +++++VSKE +KPS  TPDH RRY LSFLDQ+TVDVYNPM+YFYSA   TA +I+D
Sbjct: 35  IAMAVDIDVVSKETVKPSSPTPDHHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISD 94

Query: 61  QLKSSLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELNK 120
            LK SLS +LSHYYPLAGR  Y +L IDCNDAGVPF+E RV NCRL DV NT FPTELNK
Sbjct: 95  HLKESLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRV-NCRLSDVMNTAFPTELNK 154

Query: 121 LIPFQLDELDEVSMGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRGETGAP 180
           L+PF+LD LD+VSMGVQLNVFECGGVAVGICISHKI DALSF   VNGWAAVCRGE    
Sbjct: 155 LLPFELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVCRGE---K 214

Query: 181 RGL--HLTSAEAFPPAKTPVYNTRISILRERAAKRYRIDGGNVEAIRSKYEERKGGENER 240
            GL  HL+++E FPP KTP+YNTR SI R+R AKRY+ID  ++E IR++Y E    EN+R
Sbjct: 215 EGLETHLSASELFPPNKTPIYNTRTSIFRQRVAKRYQIDAASIETIRAQYAEYFAMENQR 274

Query: 241 RVSRLEALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLDPPLPEYA 300
           R SR+EALSAFLYGRF+AAIK  SN    N +++SEKKI+ VCHAVNIR RLDPP+P+YA
Sbjct: 275 RPSRVEALSAFLYGRFIAAIKGASNSNPANGNSESEKKIFLVCHAVNIRSRLDPPVPDYA 334

Query: 301 FGNYYRSTFALPSTEILNDHYCYGLVNKTREEIAKIDKEYLRNLQESTKFLDIMKKTVAQ 360
           FGNYYR+TFA+PS  ILND YCY LV   R+EIAKIDK YL  LQES+KFL+ MKK  AQ
Sbjct: 335 FGNYYRATFAVPSETILNDDYCYDLVKLARDEIAKIDKSYLERLQESSKFLEAMKKAAAQ 394

Query: 361 FTSGELISCAFTSLCRMPLYDADFGWGKPVWIGSPALTFKNLFVLMDEKDGDGIDVYVHL 420
           F++GELISC+FTSLCRMP+YDADFGWGKP WI SPAL FKNLFVLMD+KDGDG+DVYVHL
Sbjct: 395 FSTGELISCSFTSLCRMPIYDADFGWGKPAWISSPALIFKNLFVLMDKKDGDGVDVYVHL 454

Query: 421 EEEHMMKLEVDEVFLKYAKLPSN 440
           + E+M  LE DE FLKYAKLPSN
Sbjct: 455 KPENMEILESDEKFLKYAKLPSN 473

BLAST of Sed0003159 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.1e-59
Identity = 141/437 (32.27%), Postives = 233/437 (53.32%), Query Frame = 0

Query: 6   EMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFY-----SAVTATKITDQL 65
           +M+I+S+E+I+PS  TP  L+ + LS LDQ+ +  + P++ FY     S +   + ++ L
Sbjct: 4   QMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNLDRAQRSENL 63

Query: 66  KSSLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELNKLI 125
           K SLS +L+ +YPLAGR   ++  +DCND+GVPF+EARV + +L +        ELN+ +
Sbjct: 64  KRSLSTVLTQFYPLAGRIN-INSSVDCNDSGVPFLEARV-HSQLSEAIKNVAIDELNQYL 123

Query: 126 PFQ-------LDELDEVSMGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRG 185
           PFQ            ++ + V+++ FECGG A+G+CISHKI DALS    +N W A C+ 
Sbjct: 124 PFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATCQE 183

Query: 186 ETGAPRGLHLTSAEAFPPAKT---PVYNTRISILRERAAKRYRIDGGNVEAIRSKYEERK 245
           ET   +      +  FPP ++   P +    +I+     KR+  D   +EA++++     
Sbjct: 184 ETDIVQPNFDLGSHHFPPMESIPAPEFLPDENIV----MKRFVFDKEKLEALKAQL--AS 243

Query: 246 GGENERRVSRLEALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLDP 305
                +  SR++ + A ++ +F+   +A  + K +               AVN+R R++P
Sbjct: 244 SATEVKNSSRVQIVIAVIWKQFIDVTRAKFDTKNK----------LVAAQAVNLRSRMNP 303

Query: 306 PLPEYAFGNYYRSTFALPSTEILNDHYCYGLVNKTREEIAKIDKEYLRNLQESTKFLDIM 365
           P P+ A GN     +A+       D     LV   +  +AKID E+++ LQ+   +LD  
Sbjct: 304 PFPQSAMGNIATMAYAVAE----EDKDFSDLVGPLKTSLAKIDDEHVKELQKGVTYLDYE 363

Query: 366 KKTVAQFTSGELISCAFTSLCRMPLYDADFGWGKPVWIGSPALTFKNLFVLMDEKDGDGI 425
            +    F        +F+S CR+  YD DFGWGKPV + +  +  KNL  LMD ++ DG+
Sbjct: 364 AEPQELF--------SFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGM 410

Query: 426 DVYVHLEEEHMMKLEVD 428
           + ++ + E+ M  L  D
Sbjct: 424 EAWISMAEDEMSMLSSD 410

BLAST of Sed0003159 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 219.9 bits (559), Expect = 5.6e-56
Identity = 142/434 (32.72%), Postives = 221/434 (50.92%), Query Frame = 0

Query: 6   EMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFY-----SAVTATKITDQL 65
           +ME VS+E+I PS  TP  L+ Y +S LDQ+ +  + P + FY     S +   + +  L
Sbjct: 4   QMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDPAQTSQHL 63

Query: 66  KSSLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELNKLI 125
           K SLS +L+H+YPLAGR   V+  +DCND+GVPFVEARV       + N     +L++ +
Sbjct: 64  KQSLSKVLTHFYPLAGRIN-VNSSVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLDQYL 123

Query: 126 PF------QLDELDEVSMGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRGE 185
           P       +++  ++V + V+++ FECGG A+G+ +SHKI D LS    +N W A CRGE
Sbjct: 124 PSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTATCRGE 183

Query: 186 TGAPRGLHLTSAEAFPPA-KTPVYNTRISILRERAAKRYRIDGGNVEAIRSKYEERKGGE 245
           T         +A  FPP   TP  +  +        KR+  D   + A+R+   +     
Sbjct: 184 TEIVLPNFDLAARHFPPVDNTP--SPELVPDENVVMKRFVFDKEKIGALRA---QASSAS 243

Query: 246 NERRVSRLEALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLDPPLP 305
            E+  SR++ + A+++   +   +A    K +          + V  AVN+R R++PPLP
Sbjct: 244 EEKNFSRVQLVVAYIWKHVIDVTRAKYGAKNK----------FVVVQAVNLRSRMNPPLP 303

Query: 306 EYAFGNYYRSTFALPSTEILNDHYCYGLVNKTREEIAKIDKEYLRNLQESTKFLDIMKKT 365
            YA GN     FA    E   D     L+   R  + K + ++   L +    L      
Sbjct: 304 HYAMGNIATLLFAAVDAEWDKDF--PDLIGPLRTSLEKTEDDHNHELLKGMTCL------ 363

Query: 366 VAQFTSGELISCAFTSLCRMPLYDADFGWGKPVWIGSPALTFKNLFVLMDEKDGDGIDVY 425
             +    EL+S  FTS CR+  YD DFGWGKP+   +     +N  +LMD + GDG++ +
Sbjct: 364 -YELEPQELLS--FTSWCRLGFYDLDFGWGKPLSACTTTFPKRNAALLMDTRSGDGVEAW 410

Query: 426 VHLEEEHMMKLEVD 428
           + + E+ M  L V+
Sbjct: 424 LPMAEDEMAMLPVE 410

BLAST of Sed0003159 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 5.6e-48
Identity = 154/451 (34.15%), Postives = 223/451 (49.45%), Query Frame = 0

Query: 1   MAFEMEMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFYS---AVTATKIT 60
           MA +M +EIVSK  IKPS  TP  LRRY LS +DQ        + +FY+   +  ++KI 
Sbjct: 1   MASKM-VEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFYTYNPSHESSKIG 60

Query: 61  DQLKSSLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELN 120
           D LK+SLS  L  YY  AGR    D  IDCND GV FVE R+ + R+ D+         +
Sbjct: 61  DLLKNSLSKTLVSYYQFAGRLIENDY-IDCNDEGVEFVEVRI-HGRMNDILKRGKSFATD 120

Query: 121 KLIPFQLDELDEVS-MGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRGETG 180
            ++P ++  L E S + VQL+ F+CGG+A+G   SHK+ D +S +  +  WA+     T 
Sbjct: 121 LVLPTRIIALHEDSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWASSTSLSTF 180

Query: 181 APRGLHLTSAEAFPPAKTPVY-NTRISILRERAAKRYRIDGGNVEAIRSKYEERKGGENE 240
                 LT+   FPP    +  N  I   ++   KR+      +E ++SK  E       
Sbjct: 181 HKPTPLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIE----YGI 240

Query: 241 RRVSRLEALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLDPPLPEY 300
           ++ SR+E ++AFL        +  +N           KK Y +  AVN+RP L   LP+ 
Sbjct: 241 QKPSRVEVVTAFL-------CQCAAN------CDLPRKKPYAIISAVNLRPYL--ALPQN 300

Query: 301 AFGNYYRSTFALPSTEILNDHYCYGLVNKTREEIAK-----IDKE---YLRNLQESTKFL 360
           + GN +   F +    +  D+    L++K R    K     I KE   Y   +QE TK L
Sbjct: 301 SIGNIFSFYFCINDEGM--DNQFSALISKLRNGKQKLLENIISKEKLTYESQMQELTKCL 360

Query: 361 DIMKKTVAQFTSGELISCAFTSLCRMPLYDADFGWGKPVWIGSPALTFKNLFVLMDEKDG 420
           D       Q     L +   +S CR P+YD DFGWGKP+ +       K+L +LMD  +G
Sbjct: 361 D-------QLNISSLDTYFCSSWCRFPVYDIDFGWGKPILVSPFQPHVKDLILLMDSPEG 420

Query: 421 DGIDVYVHLEEEHMMKLEVDEVFLKYAKLPS 439
           DGI+  + +EE+ M   E +E    +A L S
Sbjct: 421 DGIEALITMEEKKMAAFEKNEELRSFAYLDS 420

BLAST of Sed0003159 vs. ExPASy Swiss-Prot
Match: I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 5.3e-46
Identity = 150/470 (31.91%), Postives = 232/470 (49.36%), Query Frame = 0

Query: 7   MEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFYSAV-----TATKITDQ-- 66
           +E++SKE IKP   TP  LR Y +S LDQ +  VY P++ FY A      T +K  D   
Sbjct: 9   VEVISKETIKPRNPTPYQLRNYNMSLLDQYSSLVYVPIILFYPAASDANSTGSKHHDDLH 68

Query: 67  -LKSSLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELNK 126
            LK SLS+ L H+YP+AGR K  ++ +DCND G+ F E R+   R+CD         L+ 
Sbjct: 69  LLKRSLSETLVHFYPMAGRMK-DNMTVDCNDEGIDFFEVRIKG-RMCDFM-MKSDAHLSL 128

Query: 127 LIPFQL---DELDEVSMGVQLNVFECGGVAVGICISHKIGDA---LSFINAVNGWAAVCR 186
           L+P ++   + + E  + VQ+N+F+CGG A+  CIS+KI DA   ++FI ++ G   + R
Sbjct: 129 LLPSEVASTNFVKEAQVIVQVNMFDCGGTAICFCISNKIADACTMITFIRSLAGTTNIAR 188

Query: 187 --GETGAPRGLH-----LTSAEAFPP----AKTPVYNTRISILRER-AAKRYRIDGGNVE 246
                 AP           S   FPP    A    Y T+ S   ++  +KR+  D   + 
Sbjct: 189 RGSSIAAPTTNQNLVPSFDSTSLFPPSEQLASQVSYPTQDSTSVDKLVSKRFVFDAAKIT 248

Query: 247 AIRSKYEERKGGENE-RRVSRLEALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVC 306
           + R K +     + +  R +R+E +SA ++    +A+K+                I  V 
Sbjct: 249 SAREKLQSLMHDKYKCHRPTRVEVVSALIW---KSAVKSA-----------PPGSISTVT 308

Query: 307 HAVNIRPRLDPPLPEYAFGNY-YRSTFALPSTEILNDHYCYGLVNKT-REEIAKIDK--E 366
           HA+N R ++DPPL + +FGN     T  LP+T     +     V+ T  EE   +D+  +
Sbjct: 309 HAMNFRKKMDPPLQDASFGNLCVVVTAVLPATTATTTNPATKKVSSTSNEEQVALDELSD 368

Query: 367 YL----RNLQESTKFLDIMKKTVAQFTSGELISCA-------------FTSLCRMPLYDA 426
           ++    R + +       M+K + +F  G   S A              TS C+   Y+A
Sbjct: 369 FVALLRREIDKVKGDKGCMEKIIQKFIYGHDASVAKDSDVEDKVTALFMTSWCKFGFYEA 428

BLAST of Sed0003159 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.2e-45
Identity = 141/478 (29.50%), Postives = 230/478 (48.12%), Query Frame = 0

Query: 3   FEMEMEIVSKEMIKPSCRTPDHLRRYALSFLDQ-ITVDVYNPMVYFYSAVTATKI----- 62
           +   +E++SKE IKP+  TP  L+ + LS LDQ   +  Y P++ FY A  A        
Sbjct: 5   YSAAVEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNH 64

Query: 63  ---TDQLKSSLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFP 122
               D LKSSLS  L H+YP+AGR    ++ +DC+D G+ F + ++   ++C+  + P  
Sbjct: 65  HDDLDLLKSSLSKTLVHFYPMAGR-MIDNILVDCHDQGINFYKVKIRG-KMCEFMSQP-D 124

Query: 123 TELNKLIPFQLDELD---EVSMGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAV 182
             L++L+P ++       E  + VQ+N+F+CGG A+   +SHKI DA +    +  WA+ 
Sbjct: 125 VPLSQLLPSEVVSASVPKEALVIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWAST 184

Query: 183 CR-----GETGAPRGLHL----TSAEAFPPAKTPVYNTRISIL-----------RERAAK 242
            +     G T A     L     SA  FPP++     + +S +            +  +K
Sbjct: 185 TKTSRSGGSTAAVTDQKLIPSFDSASLFPPSERLTSPSGMSEIPFSSTPEDTEDDKTVSK 244

Query: 243 RYRIDGGNVEAIRSKYEE-RKGGENERRVSRLEALSAFLYGRFVAAIKAVSNDKGENEST 302
           R+  D   + ++R K +         RR +R+E +++ ++             K   +ST
Sbjct: 245 RFVFDFAKITSVREKLQVLMHDNYKSRRQTRVEVVTSLIW-------------KSVMKST 304

Query: 303 KSEKKIYFVCHAVNIRPRLDPPLPEYAFGNYYRSTFA-LPSTEILNDHYCYGLVNKTREE 362
            +   +  V HAVN+R ++DPPL + +FGN   +  A LP+T     +     +N T  E
Sbjct: 305 PA-GFLPVVHHAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSE 364

Query: 363 IAKIDKEYLRNLQESTKFLDIMK-------KTVAQFTSG-----------ELISCAFTSL 422
              +  E    + +    +D +K       K +  F SG           E+I+   +S 
Sbjct: 365 SQVVLHELHDFIAQMRSEIDKVKGDKGSLEKVIQNFASGHDASIKKINDVEVINFWISSW 424

Query: 423 CRMPLYDADFGWGKPVWIG-SPAL-TFKNLFVLMDEKDGDGIDVYVHLEEEHMMKLEV 427
           CRM LY+ DFGWGKP+W+   P +   KN F + D K G+GI+V+    E+ M K E+
Sbjct: 425 CRMGLYEIDFGWGKPIWVTVDPNIKPNKNCFFMNDTKCGEGIEVWASFLEDDMAKFEL 465

BLAST of Sed0003159 vs. ExPASy TrEMBL
Match: A0A6J1K6H0 (vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111492737 PE=3 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 1.3e-180
Identity = 314/437 (71.85%), Postives = 360/437 (82.38%), Query Frame = 0

Query: 5   MEMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFYSA--VTATKITDQLKS 64
           +++E+VSKE +KPS  TPDH RRY LSFLDQ+TVDVYNPM+YFYSA   TA +I+D LK 
Sbjct: 3   VDIEVVSKETVKPSSPTPDHHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAGEISDHLKK 62

Query: 65  SLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELNKLIPF 124
           SLS +LSHYYPLAGR  Y +L IDCNDAGVPF+E RV NCRL DV NT FPTELNKL+PF
Sbjct: 63  SLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRV-NCRLSDVMNTAFPTELNKLLPF 122

Query: 125 QLDELDEVSMGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRGETGAPRGLH 184
           +LD LD+VSMGVQLNVFECGGVAVGICISHKI DALSF   VNGWAAVCRGE  A    H
Sbjct: 123 ELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVCRGEKEALE-TH 182

Query: 185 LTSAEAFPPAKTPVYNTRISILRERAAKRYRIDGGNVEAIRSKYEERKGGENERRVSRLE 244
           L+S+E FPP+KTP+YNTR SI R+R AKRY+ID  ++E IR++Y +    EN+RR SR+E
Sbjct: 183 LSSSELFPPSKTPIYNTRTSIFRQRVAKRYQIDAASIETIRAQYADYFAMENQRRPSRVE 242

Query: 245 ALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLDPPLPEYAFGNYYR 304
           ALSAFLYGRF+AAIK  SN    N  ++SEKKI+ VCHAVNIR RLDPP+P+YAFGNYYR
Sbjct: 243 ALSAFLYGRFIAAIKGASNSNPANGISESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR 302

Query: 305 STFALPSTEILNDHYCYGLVNKTREEIAKIDKEYLRNLQESTKFLDIMKKTVAQFTSGEL 364
           +TFA+PS  ILND YCY LV   REEIAKIDK YL  LQES+KFL+ MKK  AQF++GEL
Sbjct: 303 ATFAVPSETILNDDYCYDLVKLAREEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL 362

Query: 365 ISCAFTSLCRMPLYDADFGWGKPVWIGSPALTFKNLFVLMDEKDGDGIDVYVHLEEEHMM 424
           ISC+FTSLCRMP+YDADFGWGKP WI SPAL FKNLFVLMD+ DGDG+DVYVHL+ E+M 
Sbjct: 363 ISCSFTSLCRMPIYDADFGWGKPAWISSPALIFKNLFVLMDKNDGDGVDVYVHLKPENME 422

Query: 425 KLEVDEVFLKYAKLPSN 440
            LE DE FLKYAKLPSN
Sbjct: 423 ILEADEKFLKYAKLPSN 437

BLAST of Sed0003159 vs. ExPASy TrEMBL
Match: A0A6J1GWR1 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111458272 PE=3 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 1.3e-180
Identity = 314/437 (71.85%), Postives = 361/437 (82.61%), Query Frame = 0

Query: 5   MEMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFYSA--VTATKITDQLKS 64
           +++E+VSKE +KPS  TPDH RRY LSFLDQ+TVDVYNPM+YFYSA   TA +I++ LK 
Sbjct: 3   VDIEVVSKETVKPSSPTPDHHRRYGLSFLDQVTVDVYNPMIYFYSAGSATAAEISNHLKE 62

Query: 65  SLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELNKLIPF 124
           SLS +LSHYYPLAGR  Y +L IDCNDAGVPF+E RV NCRL DV NT FPTELNKL+PF
Sbjct: 63  SLSTVLSHYYPLAGRVNYDELFIDCNDAGVPFIETRV-NCRLSDVMNTAFPTELNKLLPF 122

Query: 125 QLDELDEVSMGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRGETGAPRGLH 184
           +LD LD+VSMGVQLNVFECGGVAVGICISHKI DALSF   VNGWAAVCRGE  A    H
Sbjct: 123 ELDALDDVSMGVQLNVFECGGVAVGICISHKISDALSFFIVVNGWAAVCRGEKEALE-TH 182

Query: 185 LTSAEAFPPAKTPVYNTRISILRERAAKRYRIDGGNVEAIRSKYEERKGGENERRVSRLE 244
           L+++E FPP KTP+YNTR SI R+R AKRY+ID  ++E IR++Y E    EN+RR SR+E
Sbjct: 183 LSASELFPPNKTPIYNTRTSIFRQRVAKRYQIDAASIETIRAQYAEYFAMENQRRPSRVE 242

Query: 245 ALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLDPPLPEYAFGNYYR 304
           ALSAFLYGRF+AAIK  SN    N S++SEKKI+ VCHAVNIR RLDPP+P+YAFGNYYR
Sbjct: 243 ALSAFLYGRFIAAIKGASNSNPANGSSESEKKIFLVCHAVNIRSRLDPPVPDYAFGNYYR 302

Query: 305 STFALPSTEILNDHYCYGLVNKTREEIAKIDKEYLRNLQESTKFLDIMKKTVAQFTSGEL 364
           +TFA+PS  ILND YCY LV   R+EIAKIDK YL  LQES+KFL+ MKK  AQF++GEL
Sbjct: 303 ATFAVPSETILNDDYCYDLVKLARDEIAKIDKSYLERLQESSKFLEAMKKAAAQFSTGEL 362

Query: 365 ISCAFTSLCRMPLYDADFGWGKPVWIGSPALTFKNLFVLMDEKDGDGIDVYVHLEEEHMM 424
           ISC+FTSLCRMP+YDADFGWGKP WI SPAL FKNLFVLMD+KDGDG+DVYVHL+ E+M 
Sbjct: 363 ISCSFTSLCRMPIYDADFGWGKPAWISSPALIFKNLFVLMDKKDGDGVDVYVHLKPENME 422

Query: 425 KLEVDEVFLKYAKLPSN 440
            LE DE FLKYAKLPSN
Sbjct: 423 ILESDEKFLKYAKLPSN 437

BLAST of Sed0003159 vs. ExPASy TrEMBL
Match: A0A5D3CZ30 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001940 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 4.6e-178
Identity = 307/442 (69.46%), Postives = 364/442 (82.35%), Query Frame = 0

Query: 1   MAFEMEMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFY---SAVTATKIT 60
           MAF  +++IVSKE IKPS  TPDHLRRY+LSFLDQ+TVDVYNPMVYF+   S  T  +I 
Sbjct: 1   MAF--QIQIVSKETIKPSSPTPDHLRRYSLSFLDQVTVDVYNPMVYFFVAGSTTTPAEIA 60

Query: 61  DQLKSSLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELN 120
           D+LK+SLSD+L+HYYPLAGR  Y +  IDCND GVPF+E RV NC L DV NTPFP+ELN
Sbjct: 61  DRLKTSLSDVLTHYYPLAGRLNYDEFFIDCNDDGVPFIETRV-NCPLSDVMNTPFPSELN 120

Query: 121 KLIPFQLDELDEVSMGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRGETGA 180
           +L+PF+LD+LD+VSMGVQLNVFECGGVAVGIC+SHKI DALSF   VNGWAA CRGE  A
Sbjct: 121 RLLPFELDKLDDVSMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGEKEA 180

Query: 181 PRGLHLTSAEAFPPAKTPVYNTRISILRERAAKRYRIDGGNVEAIRSKYEERKGGENERR 240
            R  HL+SAE FPP KT V+NTR SI R+R  +RY ID  NVE IR+KY E    EN+RR
Sbjct: 181 LRA-HLSSAEVFPPTKTKVHNTRTSIFRQRVGRRYHIDAANVETIRAKYAECPAMENQRR 240

Query: 241 VSRLEALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLDPPLPEYAF 300
            SR+EALS F+Y RF+AAIKAVS ++ EN S+++EKKI+ VCH+VNIR RLDPP+P+YAF
Sbjct: 241 PSRVEALSTFIYSRFIAAIKAVSKERLENGSSETEKKIFLVCHSVNIRSRLDPPVPDYAF 300

Query: 301 GNYYRSTFALPSTEILNDHYCYGLVNKTREEIAKIDKEYLRNLQESTKFLDIMKKTVAQF 360
           GNYYR+TFA PS EILND YCY LV + REEI KI+K+YL+ LQES+KF++ MKKT +QF
Sbjct: 301 GNYYRTTFAPPSEEILNDEYCYDLVKQVREEIGKINKDYLKRLQESSKFIESMKKTASQF 360

Query: 361 TSGELISCAFTSLCRMPLYDADFGWGKPVWIGSPALTFKNLFVLMDEKDGDGIDVYVHLE 420
            +GEL+SC+FTSLCRMP+YD DFGWG+P WI SPAL FKNLFV +D+KDGDGI++YVHL 
Sbjct: 361 FTGELVSCSFTSLCRMPIYDIDFGWGQPDWISSPALLFKNLFVFIDKKDGDGIEIYVHLT 420

Query: 421 EEHMMKLEVDEVFLKYAKLPSN 440
           EEHM K E+DE FLKYAKLPSN
Sbjct: 421 EEHMKKFEIDEEFLKYAKLPSN 438

BLAST of Sed0003159 vs. ExPASy TrEMBL
Match: A0A1S3BDS3 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488922 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 4.6e-178
Identity = 307/442 (69.46%), Postives = 364/442 (82.35%), Query Frame = 0

Query: 1   MAFEMEMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFY---SAVTATKIT 60
           MAF  +++IVSKE IKPS  TPDHLRRY+LSFLDQ+TVDVYNPMVYF+   S  T  +I 
Sbjct: 1   MAF--QIQIVSKETIKPSSPTPDHLRRYSLSFLDQVTVDVYNPMVYFFVAGSTTTPAEIA 60

Query: 61  DQLKSSLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELN 120
           D+LK+SLSD+L+HYYPLAGR  Y +  IDCND GVPF+E RV NC L DV NTPFP+ELN
Sbjct: 61  DRLKTSLSDVLTHYYPLAGRLNYDEFFIDCNDDGVPFIETRV-NCPLSDVMNTPFPSELN 120

Query: 121 KLIPFQLDELDEVSMGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRGETGA 180
           +L+PF+LD+LD+VSMGVQLNVFECGGVAVGIC+SHKI DALSF   VNGWAA CRGE  A
Sbjct: 121 RLLPFELDKLDDVSMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGEKEA 180

Query: 181 PRGLHLTSAEAFPPAKTPVYNTRISILRERAAKRYRIDGGNVEAIRSKYEERKGGENERR 240
            R  HL+SAE FPP KT V+NTR SI R+R  +RY ID  NVE IR+KY E    EN+RR
Sbjct: 181 LRA-HLSSAEVFPPTKTKVHNTRTSIFRQRVGRRYHIDAANVETIRAKYAECPAMENQRR 240

Query: 241 VSRLEALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLDPPLPEYAF 300
            SR+EALS F+Y RF+AAIKAVS ++ EN S+++EKKI+ VCH+VNIR RLDPP+P+YAF
Sbjct: 241 PSRVEALSTFIYSRFIAAIKAVSKERLENGSSETEKKIFLVCHSVNIRSRLDPPVPDYAF 300

Query: 301 GNYYRSTFALPSTEILNDHYCYGLVNKTREEIAKIDKEYLRNLQESTKFLDIMKKTVAQF 360
           GNYYR+TFA PS EILND YCY LV + REEI KI+K+YL+ LQES+KF++ MKKT +QF
Sbjct: 301 GNYYRTTFAPPSEEILNDEYCYDLVKQVREEIGKINKDYLKRLQESSKFIESMKKTASQF 360

Query: 361 TSGELISCAFTSLCRMPLYDADFGWGKPVWIGSPALTFKNLFVLMDEKDGDGIDVYVHLE 420
            +GEL+SC+FTSLCRMP+YD DFGWG+P WI SPAL FKNLFV +D+KDGDGI++YVHL 
Sbjct: 361 FTGELVSCSFTSLCRMPIYDIDFGWGQPDWISSPALLFKNLFVFIDKKDGDGIEIYVHLT 420

Query: 421 EEHMMKLEVDEVFLKYAKLPSN 440
           EEHM K E+DE FLKYAKLPSN
Sbjct: 421 EEHMKKFEIDEEFLKYAKLPSN 438

BLAST of Sed0003159 vs. ExPASy TrEMBL
Match: A0A0A0KPY9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590220 PE=3 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.8e-177
Identity = 303/438 (69.18%), Postives = 359/438 (81.96%), Query Frame = 0

Query: 5   MEMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFYSA---VTATKITDQLK 64
           +E+EIVSKE IKPS  TP+HLRRY+LSFLDQ+TVDVYNPMVYFY A    T  +I+D LK
Sbjct: 3   VEIEIVSKETIKPSSPTPEHLRRYSLSFLDQVTVDVYNPMVYFYPAGGSATPAEISDHLK 62

Query: 65  SSLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELNKLIP 124
           +SLS +L+HYYPLAG+  Y +  IDCND GVPF+E ++ NCRL DV NTPFP+E+NK +P
Sbjct: 63  TSLSHVLTHYYPLAGKVNYDEFFIDCNDNGVPFIETKI-NCRLSDVMNTPFPSEVNKFLP 122

Query: 125 FQLDELDEVSMGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRGETGAPRGL 184
           F+LD+LDEVSMGVQLNVFECGGV +GIC+SHKI DALS    VN WAA CRGE    R  
Sbjct: 123 FELDQLDEVSMGVQLNVFECGGVVIGICVSHKISDALSLFIVVNEWAAYCRGEKEVVRA- 182

Query: 185 HLTSAEAFPPAKTPVYNTRISILRERAAKRYRIDGGNVEAIRSKYEERKGGENERRVSRL 244
           HL+SAE FPP KT +YNTR SI R+R A+RY IDG NVE+IR+KY E    EN+RR SR+
Sbjct: 183 HLSSAELFPPTKTGLYNTRTSIFRQRVARRYEIDGENVESIRAKYAECSAMENQRRPSRV 242

Query: 245 EALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLDPPLPEYAFGNYY 304
           EALSAF+Y RF+AAIKAVS+ + EN  + SEKKI+ VCH+VNIR RLDPP+ +YAFGNYY
Sbjct: 243 EALSAFIYSRFIAAIKAVSSSELENGKSGSEKKIFLVCHSVNIRSRLDPPVVDYAFGNYY 302

Query: 305 RSTFALPSTEILNDHYCYGLVNKTREEIAKIDKEYLRNLQESTKFLDIMKKTVAQFTSGE 364
           R+TFA+PS  ILND+YCY LV + R EI KIDK+YL+ LQE +KFLD MKKT  QF++GE
Sbjct: 303 RTTFAVPSENILNDNYCYDLVKQARNEIGKIDKDYLKRLQEGSKFLDSMKKTATQFSTGE 362

Query: 365 LISCAFTSLCRMPLYDADFGWGKPVWIGSPALTFKNLFVLMDEKDGDGIDVYVHLEEEHM 424
           L+SC+FTSLCRMP+YDADFGWGKP WI SPAL FKNLFV +D+KDGDG+D+YVHL+EEHM
Sbjct: 363 LVSCSFTSLCRMPIYDADFGWGKPAWISSPALMFKNLFVFIDKKDGDGVDIYVHLKEEHM 422

Query: 425 MKLEVDEVFLKYAKLPSN 440
            K EVDE FLKYAKLPSN
Sbjct: 423 NKFEVDEEFLKYAKLPSN 438

BLAST of Sed0003159 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 315.8 bits (808), Expect = 5.4e-86
Identity = 174/436 (39.91%), Postives = 263/436 (60.32%), Query Frame = 0

Query: 5   MEMEIVSKEMIKPSCRTPDHLR-RYALSFLDQITVDVYNPMVYFY---SAVTATKITDQL 64
           +E+ + S+E++KPS R  +H    + LSFLDQ+   ++ P ++FY   + ++  + +D +
Sbjct: 3   LEITVTSQELVKPSPRNLNHPPCHHHLSFLDQLAPPIFMPFLFFYHNKTNLSDKERSDHI 62

Query: 65  KSSLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELNKLI 124
           KSSLS++L+ YYPLAGR K     + CND GV FVEA+  +C +  +   P P ELNKL 
Sbjct: 63  KSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKA-DCNMSQILENPNPNELNKLH 122

Query: 125 PFQLDELDEVSMGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRGETGAPRG 184
           PF+  E+ +V + VQL  FECGG+A+GI +SHK+ DALS +  VN WAA  RG+T     
Sbjct: 123 PFEFHEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFARGQTDEIIT 182

Query: 185 LHLTSAEAFPPAKTPVYNTRISILRER-AAKRYRIDGGNVEAIRSKYEERKGGENERRVS 244
                A+ FPP      N    I +E    +R+     +VE++R    ER  G  + R +
Sbjct: 183 PSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLR----ERFSGNKKIRAT 242

Query: 245 RLEALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLDPPLPEYAFGN 304
           R+E LS F++ RF+A+          N   K+  KIY + H VN+R + DP +P+  FGN
Sbjct: 243 RVEVLSVFIWSRFMAS---------TNHDDKT-GKIYTLIHPVNLRRQADPDIPDNMFGN 302

Query: 305 YYRSTFALPSTEI-LNDHYCYGLVNKTREEIAKIDKEYLRNLQESTK-FLDIMKKTVAQF 364
             R +  +P   I  ND     LV++ REEI KID  Y++ LQE  +  L+ + K  + F
Sbjct: 303 IMRFSVTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQASGF 362

Query: 365 TSGELISCAFTSLCRMPLYDADFGWGKPVWIGSPALTFKNLFVLMDEKDGDGIDVYVHLE 424
            +GE++S +FTSLC+ P+Y+ADFGWGKP+W+ S  +++KNL   +D K+GDGI+ +++L+
Sbjct: 363 VNGEIVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWINLD 422

Query: 425 EEHMMKLEVDEVFLKY 434
           +  M + E DE  L+Y
Sbjct: 423 QNDMSRFEADEELLRY 423

BLAST of Sed0003159 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 245.7 bits (626), Expect = 6.8e-65
Identity = 151/448 (33.71%), Postives = 247/448 (55.13%), Query Frame = 0

Query: 5   MEMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFYSAVTATKITDQ---LK 64
           M +++VS+++IKPS  TP+HL+++ LS L+Q+   ++ PMV+FYSA  + K T+Q   LK
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQLQMLK 60

Query: 65  SSLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELNKLIP 124
            SLS+ L+H+YPLAGR K  ++ IDCND+G  F+EARV N  L ++   P    L +LIP
Sbjct: 61  KSLSETLTHFYPLAGRLK-GNISIDCNDSGADFLEARV-NSPLSNLLLEPSSDSLQQLIP 120

Query: 125 FQLDELDEVS--MGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAV-CRGETGAP 184
             +D ++  +  +  Q + FECG +++G+CISHK+ DA S    +  WAA+  RG     
Sbjct: 121 TSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGSIKTI 180

Query: 185 RGLHLTSAEAFPP------AKTPVYNTRISILRERAAKRYRIDGGNVEAIRSKYEERKGG 244
                 + + FPP      +  PV    I ++ +  +KR+  D  +++A+++K       
Sbjct: 181 GAPVFDTVKIFPPGNFSETSPAPVVEPEI-MMNQTLSKRFIFDSSSIQALQAK----ASS 240

Query: 245 ENERRVSRLEALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLDPPL 304
               + +R+EA+SA ++   + A + VS             K   + ++V++R R+ PP 
Sbjct: 241 FEVNQPTRVEAVSALIWKSAMKATRTVS----------GTSKPSILANSVSLRSRVSPPF 300

Query: 305 PEYAFGNYYRSTFALPSTEILNDHYCYGLVNKTREEIAKIDKEYLRNLQESTKFLDIM-- 364
            + + GN   S FA  + E +N      LV+K R+   +    ++  L  +    +I+  
Sbjct: 301 TKNSIGNLV-SYFAAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICS 360

Query: 365 --KKTVAQFTSGELISCAFTSLCRMPLYDADFGWGKPVWIGSPALTFKNLFVLMDEKDGD 424
             K+      SG+     F+S CR  LY+ DFGWGKPVW+G P++  KN+  L+D K+  
Sbjct: 361 YQKEAGDMIASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAG 420

Query: 425 GIDVYVHLEEEHMMKLEVDEVFLKYAKL 437
           GI+ +V+L E+ M   E D   L++A L
Sbjct: 421 GIEAWVNLNEQEMNLFEQDRELLQFASL 430

BLAST of Sed0003159 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 204.1 bits (518), Expect = 2.3e-52
Identity = 147/453 (32.45%), Postives = 230/453 (50.77%), Query Frame = 0

Query: 5   MEMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFYS---AVTATKITDQLK 64
           M++E ++KE+IKPS  TP+ L+   LS  D I   VY     FY+    ++  + + +LK
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPEQSSHKLK 63

Query: 65  SSLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELNKLIP 124
           +SL++ L+ +YPLAGR K V   IDCND G  FV+ARV N  L D   +P    L +L+P
Sbjct: 64  TSLAETLTKFYPLAGRIKGV--TIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQQLLP 123

Query: 125 FQLDELDE--------VSMGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRG 184
             LD +D+          + V+   F CGG+A+G+CI+HKI DA S    +  WAA+ RG
Sbjct: 124 --LDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMARG 183

Query: 185 ETG-APRGLHLTSAEAFPPA----KTPVYNTRISILRERAAKRYRIDGGNVEAIRSKYEE 244
           E G         +A  +PPA    K PV      I   +  KR+      +E +R+K   
Sbjct: 184 EAGDIVADPVFAAANFYPPANELFKFPVDENANKI--SQITKRFVFSASKLEELRTKAAS 243

Query: 245 RKGGENERRVSRLEALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRL 304
               +   R  R+E+++A L+  FVAA  + +N          + K+  +    N+RP++
Sbjct: 244 E---DFVGRPKRVESVTALLWKAFVAAASSNTN--------ACDMKV--LIQPSNLRPKI 303

Query: 305 DPPLPEYAFGNYYRSTFAL--PSTEILNDHYCYGLVNKTREEIAKIDKEY--LRNLQEST 364
              L E   GN   S+  L     E +        + K  +++  + KE     +    +
Sbjct: 304 PSLLAESLIGNIMFSSVVLGISQQEEIKIEKAVRDLRKQGDDLQNVIKEEGGSSSAMIGS 363

Query: 365 KFLDIMKKTVAQFTSGELISCAFTSLCRMPLYDADFGWGKPVWI-GSPALTFKNLFVLMD 424
           K  ++M    ++ +         +S C++PLY+A FGWG PVWI G+ A   +N+ +L+D
Sbjct: 364 KLANLMLTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLID 423

Query: 425 EKDGDGIDVYVHLEEEHMMKLEVDEVFLKYAKL 437
            KDG GI+ +V L EE+M+ LE +   L +A +
Sbjct: 424 SKDGQGIEAFVTLPEENMLSLEQNTELLAFASV 437

BLAST of Sed0003159 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 192.6 bits (488), Expect = 6.8e-49
Identity = 141/455 (30.99%), Postives = 223/455 (49.01%), Query Frame = 0

Query: 5   MEMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFYS---AVTATKITDQLK 64
           M++E +SKE+IKPS  TP++L+   LS  D I   VY     FY+    ++    + +LK
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLISQEHTSHKLK 65

Query: 65  SSLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTELNKLIP 124
           +SLS+ L+ +YPLAGR     + +DC D G  FV+ARV NC L +    P    L +L+P
Sbjct: 66  TSLSETLTKFYPLAGR--ITGVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQLLP 125

Query: 125 FQLDELDEVSMG------VQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRGE- 184
             + +   V+        V+   F CGG+A+GICI+HKI DA S    +  WAA  RGE 
Sbjct: 126 LDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATARGEN 185

Query: 185 -TGAPRGLHLTSAEAFPPA----KTPVYNTRISILRERAAKRYRIDGGNVEAIRSKYEER 244
              A        A  +PPA    K P      +  R    KR+  +   VE +R+K    
Sbjct: 186 DAAAMESPVFAGANFYPPANEAFKLPA--DEQAGKRSSITKRFVFEASKVEDLRTKAASE 245

Query: 245 KGGENERRVSRLEALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPRLD 304
              E   + +R+E+++A ++  FVA+ K          +T  + K+  +    N+R ++ 
Sbjct: 246 ---ETVDQPTRVESVTALIWKCFVASSK----------TTTCDHKV--LVQLANLRSKIP 305

Query: 305 PPLPEYAFGNYYRSTFALP---STEILNDHYCYGLVNKTREEIAKIDKEYLRNLQES--- 364
             L E + GN   S+  L      E+  +     L  K +EE+  +  +   +   S   
Sbjct: 306 SLLQESSIGNLMFSSVVLSIGRGGEVKIEEAVRDL-RKKKEELGTVILDEGGSSDSSSMI 365

Query: 365 -TKFLDIMKKTVAQFTSGELISCAFTSLCRMPLYDADFGWGKPVW-IGSPALTFKNLFVL 424
            +K  ++M    ++ +         +S C++PLY+A FGW  PVW +G+ +    NL +L
Sbjct: 366 GSKLANLMLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLAML 425

Query: 425 MDEKDGDGIDVYVHLEEEHMMKLEVDEVFLKYAKL 437
           +D KDG GI+ +V L EE+M   E +   L +A +
Sbjct: 426 IDSKDGQGIEAFVTLPEENMSSFEQNPELLAFATM 440

BLAST of Sed0003159 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 186.8 bits (473), Expect = 3.8e-47
Identity = 143/455 (31.43%), Postives = 224/455 (49.23%), Query Frame = 0

Query: 4   EMEMEIVSKEMIKPSCRTPDHLRRYALSFLDQITVDVYNPMVYFYSA-------VTATKI 63
           E  +EI+S+E++KPS  TPD  R   LS LD ++  +Y   + FY+A        +  + 
Sbjct: 2   EKNVEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEET 61

Query: 64  TDQLKSSLSDMLSHYYPLAGRAKYVDLCIDCNDAGVPFVEARVLNCRLCDVTNTPFPTEL 123
           + +LK SLS  L  +YPLAGR   +   ++CND G  F+EARV +  L +    P P  L
Sbjct: 62  SLKLKKSLSKTLPIFYPLAGR--IIGSFVECNDEGAVFIEARV-DHLLSEFLKCPVPESL 121

Query: 124 NKLIPFQLDELDEVS---MGVQLNVFECGGVAVGICISHKIGDALSFINAVNGWAAVCRG 183
             LIP +    + V+   + +Q N F CGG+ + IC+SHKI DA S    + GWA   RG
Sbjct: 122 ELLIPVEAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRG 181

Query: 184 ETGAPRGLHLTSAEAFP------PAKTPVYNTRISILRERAAKRYRIDGGNVEAIRSKYE 243
             G       T++E FP      P+K       +  +     KR+  D   ++ +R+K  
Sbjct: 182 -LGITLIPSFTASEVFPKPLDELPSKPMDRKEEVEEM-SCVTKRFVFDASKIKKLRAKAS 241

Query: 244 ERKGGENERRVSRLEALSAFLYGRFVAAIKAVSNDKGENESTKSEKKIYFVCHAVNIRPR 303
                   +  +R+EA++A L+ R V  +  +S       S      +  +   VN+R +
Sbjct: 242 RNL----VKNPTRVEAVTA-LFWRCVTKVSRLS-------SLTPRTSVLQI--LVNLRGK 301

Query: 304 LDPPLPEYAFGNYYRSTFALPSTEILNDHYCYGLVNKTREEIAKIDKEYLRNLQE----S 363
           +D  L E   GN       L    + N+      +    +EI +  + +  N +E    S
Sbjct: 302 VD-SLCENTIGN------MLSLMILKNEEAAIERIQDVVDEIRRAKEIFSLNCKEMSKSS 361

Query: 364 TKFLDIMKKTVAQFTSG-ELISCAFTSLCRMPLYDADFGWGKPVWI-GSPALTFKNLFVL 423
           ++  +++++    +  G E+      S C++ LYDADFGWGKPVW+ G     FKNL +L
Sbjct: 362 SRIFELLEEIGKVYGRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLL 421

Query: 424 MDEKDGDGIDVYVHLEEEHMMKLEVDEVFLKYAKL 437
           +D KDG+GI+ ++ L EE M   E D+  L+ A L
Sbjct: 422 IDTKDGEGIEAWITLTEEQMSLFECDQELLESASL 430