Sed0001255 (gene) Chayote v1

BLAST of Sed0001255 vs. NCBI nr
Match: XP_038890957.1 (diacylglycerol kinase 7-like [Benincasa hispida])

HSP 1 Score: 895.6 bits (2313), Expect = 1.9e-256
Identity = 433/493 (87.83%), Postives = 460/493 (93.31%), Query Frame = 0

Query: 1   MDSPTSKGGGDAKTRIAARTSTIESFKGCGVTGMRIDKEELRKKLRMPEYLRLAMRDSIR 60
           M+SP+S+G GD+ TRIAAR+S IESFKGCG+TG+RIDKEELRKKLRMP+YLR AMRDSIR
Sbjct: 1   MNSPSSRGEGDSTTRIAARSSMIESFKGCGLTGLRIDKEELRKKLRMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPGSGTVSGST------DGPPESPVVVFINPRSGGRHGHLLKERLQMLIS 120
           LQDP+AGESRLPG+ ++SGS       DGPP SP+VVFINPRSGGRHG LLKERLQMLIS
Sbjct: 61  LQDPSAGESRLPGANSISGSAFVPENDDGPPASPMVVFINPRSGGRHGRLLKERLQMLIS 120

Query: 121 DEQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180
           +EQ+FDLTDVKPHEFVKYGLRCLEL A LGDACAK+ REKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQIFDLTDVKPHEFVKYGLRCLELLAGLGDACAKDVREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLDSW 240
           ELEKK RLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG IRKLDSW
Sbjct: 181 ELEKKERLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRKLDSW 240

Query: 241 HVILSAPSSENVELQLPHCMKPTEALALDESLEIEEASSEKASCHQGVFYNYFSIGMDAQ 300
           HVILS PS ENVE  LPHCMKPTE  ALDESLEIEEA SEKASCH+GVFYNYFSIGMDAQ
Sbjct: 241 HVILSTPSGENVE--LPHCMKPTEEHALDESLEIEEALSEKASCHEGVFYNYFSIGMDAQ 300

Query: 301 VAYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKKV 360
           VAYGFHHLRNEKPYLAQGPI+NKLIYSGYSCGQGWFFT C S+P LRGLKNILRMHVKKV
Sbjct: 301 VAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPRLRGLKNILRMHVKKV 360

Query: 361 NCSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFGL 420
           NCSDWEQ++IPSSVRSLVALNLNNYGSGRHPWG+L+PEYMEK+GFV A VDDGLLEVFGL
Sbjct: 361 NCSDWEQILIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGL 420

Query: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFVEI 480
           KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKD FLQMDGEPWKQPMS+EFSTFVEI
Sbjct: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSDEFSTFVEI 480

Query: 481 KRVPSQSLMINGE 488
           K+VPS SLMI+GE
Sbjct: 481 KKVPSHSLMISGE 491

BLAST of Sed0001255 vs. NCBI nr
Match: KAG6598863.1 (Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 894.4 bits (2310), Expect = 4.3e-256
Identity = 430/493 (87.22%), Postives = 461/493 (93.51%), Query Frame = 0

Query: 1   MDSPTSKGGGDAKTRIAARTSTIESFKGCGVTGMRIDKEELRKKLRMPEYLRLAMRDSIR 60
           MDSPTS+GGGD+ TRI AR+S IESFKGCG+TGMRIDKE+LRK+LRMP+YLR AMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPGSGTVSGS------TDGPPESPVVVFINPRSGGRHGHLLKERLQMLIS 120
           LQDP AGESRLPG+ ++SGS       DGPPESP+VVFINPRSGGRHG LLKERLQMLIS
Sbjct: 61  LQDPGAGESRLPGASSISGSPFAQDTDDGPPESPMVVFINPRSGGRHGRLLKERLQMLIS 120

Query: 121 DEQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180
           +EQVFDLTDVKPHEFV+YGLRCLEL A LGDACAKETREKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLDSW 240
           ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG IR+LDSW
Sbjct: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLDSW 240

Query: 241 HVILSAPSSENVELQLPHCMKPTEALALDESLEIEEASSEKASCHQGVFYNYFSIGMDAQ 300
            V+LS+PS EN++  LPHCMKPTE  ALDESLEIEEA  EKA+CH+GVFYNYFSIGMDAQ
Sbjct: 241 QVLLSSPSGENMD--LPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFSIGMDAQ 300

Query: 301 VAYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKKV 360
           VAYGFHHLRNEKPYLAQGPI+NKLIYSGYSCGQGWFFT C S+PSLRGLKNILRMHVKK+
Sbjct: 301 VAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILRMHVKKI 360

Query: 361 NCSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFGL 420
           NCSDWE + IPSSVRSLVALNLNNYGSGRHPWG+LSPEYMEK+GFV AHVDDGLLEVFGL
Sbjct: 361 NCSDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGLLEVFGL 420

Query: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFVEI 480
           KQGWHASLVMGELISAKHIVQAAAIRFELRGGEW++ FLQMDGEPWKQPM++EFSTFVEI
Sbjct: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEFSTFVEI 480

Query: 481 KRVPSQSLMINGE 488
           KRVP QSLMI+GE
Sbjct: 481 KRVPCQSLMISGE 491

BLAST of Sed0001255 vs. NCBI nr
Match: XP_022929674.1 (diacylglycerol kinase 7-like [Cucurbita moschata] >KAG7029804.1 Diacylglycerol kinase 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 893.6 bits (2308), Expect = 7.3e-256
Identity = 430/493 (87.22%), Postives = 460/493 (93.31%), Query Frame = 0

Query: 1   MDSPTSKGGGDAKTRIAARTSTIESFKGCGVTGMRIDKEELRKKLRMPEYLRLAMRDSIR 60
           MDSPTS+GGGD+ TRI AR+S IESFKGCG+TGMRIDKE+LRK+LRMP+YLR AMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPGSGTVSGS------TDGPPESPVVVFINPRSGGRHGHLLKERLQMLIS 120
           LQDP AGESRLPG+ ++SGS       DGPPESP+VVFINPRSGGRHG LLKERLQMLIS
Sbjct: 61  LQDPGAGESRLPGASSISGSPFAQDTDDGPPESPMVVFINPRSGGRHGRLLKERLQMLIS 120

Query: 121 DEQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180
           +EQVFDLTDVKPHEFV+YGLRCLEL A LGDACAKETREKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLDSW 240
           ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG IR+LDSW
Sbjct: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLDSW 240

Query: 241 HVILSAPSSENVELQLPHCMKPTEALALDESLEIEEASSEKASCHQGVFYNYFSIGMDAQ 300
            V LS+PS EN++  LPHCMKPTE  ALDESLEIEEA  EKA+CH+GVFYNYFSIGMDAQ
Sbjct: 241 QVFLSSPSGENMD--LPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFSIGMDAQ 300

Query: 301 VAYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKKV 360
           VAYGFHHLRNEKPYLAQGPI+NKLIYSGYSCGQGWFFT C S+PSLRGLKNILRMHVKK+
Sbjct: 301 VAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILRMHVKKI 360

Query: 361 NCSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFGL 420
           NCSDWE + IPSSVRSLVALNLNNYGSGRHPWG+LSPEYMEK+GFV AHVDDGLLEVFGL
Sbjct: 361 NCSDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGLLEVFGL 420

Query: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFVEI 480
           KQGWHASLVMGELISAKHIVQAAAIRFELRGGEW++ FLQMDGEPWKQPM++EFSTFVEI
Sbjct: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEFSTFVEI 480

Query: 481 KRVPSQSLMINGE 488
           KRVP QSLMI+GE
Sbjct: 481 KRVPCQSLMISGE 491

BLAST of Sed0001255 vs. NCBI nr
Match: XP_022997408.1 (diacylglycerol kinase 7-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 891.3 bits (2302), Expect = 3.6e-255
Identity = 428/493 (86.82%), Postives = 460/493 (93.31%), Query Frame = 0

Query: 1   MDSPTSKGGGDAKTRIAARTSTIESFKGCGVTGMRIDKEELRKKLRMPEYLRLAMRDSIR 60
           MDSPTS+GGGD+ TRI AR+S IESFKGCG+TGMRIDKE+LRK+LRMP+YLR AMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPGSGTVSGS------TDGPPESPVVVFINPRSGGRHGHLLKERLQMLIS 120
           LQDP AGESRLPG+ ++SGS       DGPPESP+VVFINP+SGGRHG LLKERLQMLIS
Sbjct: 61  LQDPGAGESRLPGASSISGSPFAQDTDDGPPESPMVVFINPQSGGRHGRLLKERLQMLIS 120

Query: 121 DEQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180
           +EQVFD+TDVKPHEFV+YGLRCLEL A LGDACAKETREKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDITDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLDSW 240
           ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG IR+LDSW
Sbjct: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLDSW 240

Query: 241 HVILSAPSSENVELQLPHCMKPTEALALDESLEIEEASSEKASCHQGVFYNYFSIGMDAQ 300
            V LS+PS EN++  LPHCMKPTE  ALDESLEIEEA  EKA+CH+GVFYNYFSIGMDAQ
Sbjct: 241 QVFLSSPSGENMD--LPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFSIGMDAQ 300

Query: 301 VAYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKKV 360
           VAYGFHHLRNEKPYLAQGPI+NKLIYSGYSCGQGWFFT C S+PSLRGLKNILRMHVKK+
Sbjct: 301 VAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILRMHVKKI 360

Query: 361 NCSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFGL 420
           NCSDWE + IPSSVRSLVALNLNNYGSGRHPWG+LSPEYMEK+GFV AHVDDGLLEVFGL
Sbjct: 361 NCSDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGLLEVFGL 420

Query: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFVEI 480
           KQGWHASLVMGELISAKHIVQAAAIRFELRGGEW++ FLQMDGEPWKQPM++EFSTFVEI
Sbjct: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEFSTFVEI 480

Query: 481 KRVPSQSLMINGE 488
           KRVP QSLMI+GE
Sbjct: 481 KRVPCQSLMISGE 491

BLAST of Sed0001255 vs. NCBI nr
Match: XP_023546114.1 (diacylglycerol kinase 7-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 890.6 bits (2300), Expect = 6.2e-255
Identity = 428/493 (86.82%), Postives = 459/493 (93.10%), Query Frame = 0

Query: 1   MDSPTSKGGGDAKTRIAARTSTIESFKGCGVTGMRIDKEELRKKLRMPEYLRLAMRDSIR 60
           MDSPTS+GGGD+ TRI AR+S IESFKGCG+TGMRIDKE+LRK+LRMP+YLR AMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPGSGTVSGS------TDGPPESPVVVFINPRSGGRHGHLLKERLQMLIS 120
           LQDP AGESRLPG+ ++SGS       DGPPESP+VVFINPRSGGRHG LLKERLQMLIS
Sbjct: 61  LQDPGAGESRLPGASSISGSPFAQDTDDGPPESPMVVFINPRSGGRHGRLLKERLQMLIS 120

Query: 121 DEQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180
           +EQVFDLTDVKPHEFV+YGLRCLEL A LGDACAKETREKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLDSW 240
           ELEKKGRLP+PPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG IR+LDSW
Sbjct: 181 ELEKKGRLPLPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLDSW 240

Query: 241 HVILSAPSSENVELQLPHCMKPTEALALDESLEIEEASSEKASCHQGVFYNYFSIGMDAQ 300
            V LS+P  EN++  LPHCMKPTE  ALDESLEIEEA  EKA+CH+GVFYNYFSIGMDAQ
Sbjct: 241 QVFLSSPCGENMD--LPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFSIGMDAQ 300

Query: 301 VAYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKKV 360
           VAYGFHHLRNEKPYLAQGPI+NKLIYSGYSCGQGWFFT C S+PSLRGLKNILRMHVKK+
Sbjct: 301 VAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILRMHVKKI 360

Query: 361 NCSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFGL 420
           NCSDWE + IPSSVRSLVALNLNNYGSGRHPWG+LSPEYMEK+GFV AHVDDGLLEVFGL
Sbjct: 361 NCSDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGLLEVFGL 420

Query: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFVEI 480
           KQGWHASLVMGELISAKHIVQAAAIRFELRGGEW++ FLQMDGEPWKQPM++EFSTFVEI
Sbjct: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEFSTFVEI 480

Query: 481 KRVPSQSLMINGE 488
           KRVP QSLMI+GE
Sbjct: 481 KRVPCQSLMISGE 491

BLAST of Sed0001255 vs. ExPASy Swiss-Prot
Match: Q8VZG1 (Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 4.5e-192
Identity = 318/494 (64.37%), Postives = 388/494 (78.54%), Query Frame = 0

Query: 1   MDSPTSKGGGDAKTRIAARTSTIES--FKGCGVTGMR---IDKEELRKKLRMPEYLRLAM 60
           MDSP SK     +  +A+R ST +S   +GCG+  +    +DK ELR++L MPEYLRLAM
Sbjct: 1   MDSPVSKTDASKEKFVASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAM 60

Query: 61  RDSIRLQDPAA--GESRLPGSGTVSGSTDGPPESPVVVFINPRSGGRHGHLLKERLQMLI 120
           RD I+ +D +A      LPG        D  P +P+VVFINP SGGRHG +LKERLQ L+
Sbjct: 61  RDCIKRKDSSAIPDHLLLPGGAV----ADMAPHAPMVVFINPNSGGRHGPVLKERLQQLM 120

Query: 121 SDEQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGCL 180
           S+EQVFDLT+VKPHEFV+YGL CLE  A  GD CAKE R ++R+MVAGGDGTVGWVLGCL
Sbjct: 121 SEEQVFDLTEVKPHEFVRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCL 180

Query: 181 LELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLDS 240
            EL K G+  +PPVGVIPLGTGNDLSR+FGWGGSFPFAW+SA+KR+L RA+ GP+ +LDS
Sbjct: 181 GELNKDGKSQIPPVGVIPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDS 240

Query: 241 WHVILSAPSSENVELQLPHCMKPTEALALDESLEIEEASSEKASCHQGVFYNYFSIGMDA 300
           W +++S PS E V+   P+ +KP E   LD+ L+    +   A  ++GVFYNY SIGMDA
Sbjct: 241 WKILVSMPSGEVVD--PPYSLKPAEENELDQGLDAGIDAPPLAKAYEGVFYNYLSIGMDA 300

Query: 301 QVAYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKK 360
           QVAYGFHHLRN KPYLAQGPISNK+IYS + C QGWF T C ++P LRGL+NI+++H+KK
Sbjct: 301 QVAYGFHHLRNTKPYLAQGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKK 360

Query: 361 VNCSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFG 420
           VNCS WE++ +P +VRS+VALNL++YGSG HPWG+L P+Y+EK+GFV AH DDGL+E+FG
Sbjct: 361 VNCSQWEEIAVPKNVRSIVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFG 420

Query: 421 LKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFVE 480
            KQGWHAS VM ELISAKHI QAAA+RFELRGG+W+D FLQMDGEPWKQPMS E+STFVE
Sbjct: 421 FKQGWHASFVMAELISAKHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVE 480

Query: 481 IKRVPSQSLMINGE 488
           IK+VP QSLMIN E
Sbjct: 481 IKKVPYQSLMINNE 488

BLAST of Sed0001255 vs. ExPASy Swiss-Prot
Match: F4JQ95 (Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 1.0e-191
Identity = 320/495 (64.65%), Postives = 393/495 (79.39%), Query Frame = 0

Query: 5   TSKGGGDAKT------RIAARTSTIESFKGCGVTGMR---IDKEELRKKLRMPEYLRLAM 64
           T +  G+A T      R +A+T    + +GCG   +    IDKEELR +L MPEYLR+AM
Sbjct: 4   TPRSVGEASTTNFVAARPSAKTDDAVTMRGCGFANLALVGIDKEELRGRLAMPEYLRIAM 63

Query: 65  RDSIRLQDPA--AGESRLPGSGTVSGSTDGPPESPVVVFINPRSGGRHGHLLKERLQMLI 124
           RD I+ +D    +    LPG      + D  P +P+VVFINP+SGGRHG +LKERLQ L+
Sbjct: 64  RDCIKRKDSTEISDHLLLPG----GAAADMAPHAPMVVFINPKSGGRHGPVLKERLQQLM 123

Query: 125 SDEQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGCL 184
           ++EQVFDLT+VKPHEFV+YGL CL+  A  GD CA+E REK+R+MVAGGDGTVGWVLGCL
Sbjct: 124 TEEQVFDLTEVKPHEFVRYGLGCLDTLAAKGDECARECREKIRIMVAGGDGTVGWVLGCL 183

Query: 185 LELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLDS 244
            EL K G+  +PPVGVIPLGTGNDLSR+F WGGSFPFAW+SA+KR+L RAT G I +LDS
Sbjct: 184 GELHKDGKSHIPPVGVIPLGTGNDLSRSFSWGGSFPFAWRSAMKRTLHRATLGSIARLDS 243

Query: 245 WHVILSAPSSENVELQLPHCMKPT-EALALDESLEIEEASSEKASCHQGVFYNYFSIGMD 304
           W +++S PS E V+   P+ +KPT E  ALD++L+ +     KA  ++GVFYNYFSIGMD
Sbjct: 244 WKIVVSMPSGEVVD--PPYSLKPTIEETALDQALDADGDVPPKAKSYEGVFYNYFSIGMD 303

Query: 305 AQVAYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVK 364
           AQVAYGFHHLRNEKPYLAQGP++NK+IYS YSC QGWF T C +NP+LRGL+NI+++H+K
Sbjct: 304 AQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYSCTQGWFCTPCVNNPALRGLRNIMKIHIK 363

Query: 365 KVNCSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVF 424
           K NCS+WE++ +P SVRS+V LNL NYGSGRHPWG+L P+Y+EK+GFV AH DDGL+E+F
Sbjct: 364 KANCSEWEEIHVPKSVRSIVVLNLYNYGSGRHPWGNLRPKYLEKRGFVEAHCDDGLIEIF 423

Query: 425 GLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFV 484
           GLKQGWHAS VM E+ISAKHI QAAAIRFELRGG+WK+ FLQMDGEPWKQPM +++STFV
Sbjct: 424 GLKQGWHASFVMAEIISAKHIAQAAAIRFELRGGDWKNAFLQMDGEPWKQPMKSDYSTFV 483

Query: 485 EIKRVPSQSLMINGE 488
           EIK+VP QSLMINGE
Sbjct: 484 EIKKVPFQSLMINGE 492

BLAST of Sed0001255 vs. ExPASy Swiss-Prot
Match: Q1PDI2 (Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 3.6e-181
Identity = 299/493 (60.65%), Postives = 386/493 (78.30%), Query Frame = 0

Query: 1   MDSPTSKGGGDAKTRIAARTSTIESFKGCGVT------GMRIDKEELRKKLRMPEYLRLA 60
           M+SP+   G     R+  R S+++SF    V+       +R+ K ELR+++ +P+YLR+A
Sbjct: 1   MESPSI--GDSLTARMIPRHSSLDSFGAMKVSLLVNLASIRVSKAELRQRVMLPQYLRIA 60

Query: 61  MRDSIRLQDPAAGESRLPGSGTVSGSTDGPPESPVVVFINPRSGGRHGHLLKERLQMLIS 120
           +RD I  +D +   S        + +    PE P++VF+NP+SGGR G L+KERLQ LIS
Sbjct: 61  IRDCILRKDDSFDASSSVAPPLENNAL--TPEVPLMVFVNPKSGGRQGPLIKERLQNLIS 120

Query: 121 DEQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180
           +EQV+DLT+VKP+EF++YGL CLE FA  GD CAKE REKMR++VAGGDGTVGWVLGCL 
Sbjct: 121 EEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIREKMRIVVAGGDGTVGWVLGCLG 180

Query: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLDSW 240
           EL  + RLPVPPV ++PLGTGNDLSR+FGWGGSFPFAWKSAIKR+L RA+  PI +LDSW
Sbjct: 181 ELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAWKSAIKRTLHRASVAPISRLDSW 240

Query: 241 HVILSAPSSENVELQLPHCMKPTEALALDESLEIEEASSEKASCHQGVFYNYFSIGMDAQ 300
           +++++ PS E V+   P+ +K T+   +D++LEIE       + ++GVFYNYFSIGMDAQ
Sbjct: 241 NILITMPSGEIVD--PPYSLKATQECYIDQNLEIEGEIPPSTNGYEGVFYNYFSIGMDAQ 300

Query: 301 VAYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKKV 360
           VAYGFHHLRNEKPYLA GPI+NK+IYSGY C QGWF T C ++P LRGLKNI+ +H+KK+
Sbjct: 301 VAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFLTHCINDPGLRGLKNIMTLHIKKL 360

Query: 361 NCSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFGL 420
           + S+WE+V +P SVR++VALNL++YGSGR+PWG+L  +Y+EK+GFV A  DDGLLE+FGL
Sbjct: 361 DSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDYLEKRGFVEAQADDGLLEIFGL 420

Query: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFVEI 480
           KQGWHAS VM ELISAKHI QAAAIR E+RGG+WKD F+QMDGEPWKQPM+ ++STFV+I
Sbjct: 421 KQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDAFMQMDGEPWKQPMTRDYSTFVDI 480

Query: 481 KRVPSQSLMINGE 488
           KRVP QSL++ G+
Sbjct: 481 KRVPHQSLVVKGD 487

BLAST of Sed0001255 vs. ExPASy Swiss-Prot
Match: Q9C5E5 (Diacylglycerol kinase 5 OS=Arabidopsis thaliana OX=3702 GN=DGK5 PE=2 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.2e-70
Identity = 141/409 (34.47%), Postives = 223/409 (54.52%), Query Frame = 0

Query: 85  PESPVVVFINPRSGGRHGHLLKERLQMLISDEQVFDLTDVKPHEFVKYGLRCLELFAELG 144
           P SPV+VFIN +SGG+ G  L    + L++  QVFDL    P + ++     LE   +  
Sbjct: 37  PASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLKD-- 96

Query: 145 DACAKETREKMRVMVAGGDGTVGWVLGCLLELEKKGRLPVPPVGVIPLGTGNDLSRTFGW 204
           D  A++ REK++++VAGGDGT GW+LG + +L+       PP+  +PLGTGN+L   FGW
Sbjct: 97  DDFARQIREKLKIIVAGGDGTAGWLLGVVCDLKLS---HPPPIATVPLGTGNNLPFAFGW 156

Query: 205 GGSFPFAWKSAIKRSLDRATTGPIRKLDSWHVILSAPSSEN-------VELQLPHCMKPT 264
           G   P   ++A++  L++     + K+D+WH+++   + +          L+LPH +   
Sbjct: 157 GKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPHSLHAF 216

Query: 265 EALALDESLEIEEASSEKASCH--QGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPIS 324
             ++  + L        K  CH  +G F+NYFS+GMDAQ++Y FH  R   P   +  + 
Sbjct: 217 HRVSPTDEL-------NKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFKNQLV 276

Query: 325 NKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKKVNCSDWEQVVIPSSVRSLVALN 384
           N+  Y    C QGWF  S F +P+ R +  + ++ +   N   W+ + IP S+RS+V LN
Sbjct: 277 NQSTYVKLGCTQGWFCASLF-HPASRNIAQLAKVKIATRN-GQWQDLHIPHSIRSIVCLN 336

Query: 385 LNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFGLKQGWHASLVMGELISAKHIVQ 444
           L ++  G +PWG  +P     +G     VDDGL+EV G +  WH  +++        + Q
Sbjct: 337 LPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGTRLAQ 396

Query: 445 AAAIRFELRGGEWKDGFLQMDGEPWKQPMS-NEFSTFVEIKRVPSQSLM 484
           A  IRFE   G     F++MDGEPWKQP+  ++ +  VEI  +   +++
Sbjct: 397 ANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNML 431

BLAST of Sed0001255 vs. ExPASy Swiss-Prot
Match: F4JKI3 (Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.2e-70
Identity = 139/395 (35.19%), Postives = 222/395 (56.20%), Query Frame = 0

Query: 85  PESPVVVFINPRSGGRHGHLLKERLQMLISDEQVFDLTDVKPHEFVKYGLRCLELFAELG 144
           PE+P++VFIN +SGG+ G  L    + L++D+QVFDL    P + ++     LE   +  
Sbjct: 44  PENPILVFINSKSGGQLGAELILTYRTLLNDKQVFDLEVETPDKVLQRIYLNLERLKD-- 103

Query: 145 DACAKETREKMRVMVAGGDGTVGWVLGCLLELEKKGRLPVPPVGVIPLGTGNDLSRTFGW 204
           D+ A + R+K++++VAGGDGT GW+LG + +L        PP+  +PLGTGN+L   FGW
Sbjct: 104 DSLASKIRDKLKIIVAGGDGTAGWLLGVVSDLNLSN---PPPIATVPLGTGNNLPFAFGW 163

Query: 205 GGSFPFAWKSAIKRSLDRATTGPIRKLDSWHVIL----SAPSSENVELQLPHCMKPTEAL 264
           G   P   +S+++  L +       K+D+W +++        S ++ L+LPH        
Sbjct: 164 GKKNPGTDRSSVESFLGKVINAKEMKIDNWKILMRMKHPKEGSCDITLKLPH-------- 223

Query: 265 ALDESLEIEEASSEKASCHQGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPISNKLIY 324
           +L      ++ + E    ++G F+NYFS+GMDAQV+Y FH  R   P   +  + N+  Y
Sbjct: 224 SLPRIFPSDQENMEGYHTYRGGFWNYFSLGMDAQVSYAFHSQRKLHPERFKNQLVNQSTY 283

Query: 325 SGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKKVNCSDWEQVVIPSSVRSLVALNLNNYG 384
              SC QGWFF S F +PS + +  + ++ +   N   W  + IP S+RS+V LNL ++ 
Sbjct: 284 LKLSCTQGWFFASLF-HPSSQNIAKLAKIQICDRN-GQWNDLHIPQSIRSIVCLNLPSFS 343

Query: 385 SGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFGLKQGWHASLVMGELISAKHIVQAAAIR 444
            G +PWG  +P+    +   A  VDDGL+E+ G +  WH  +++        + QA  +R
Sbjct: 344 GGLNPWGTPNPKKQRDRSLTAPFVDDGLIEIVGFRNAWHGLILLSPNGHGTRLAQANRVR 403

Query: 445 FELRGGEWKDGFLQMDGEPWKQPM-SNEFSTFVEI 475
            E + G  K  ++++DGEPWKQP+ SN+ +  VEI
Sbjct: 404 LEFKKGAAKHAYMRIDGEPWKQPLPSNDETVMVEI 423

BLAST of Sed0001255 vs. ExPASy TrEMBL
Match: A0A6J1EPG0 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111436187 PE=3 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 3.5e-256
Identity = 430/493 (87.22%), Postives = 460/493 (93.31%), Query Frame = 0

Query: 1   MDSPTSKGGGDAKTRIAARTSTIESFKGCGVTGMRIDKEELRKKLRMPEYLRLAMRDSIR 60
           MDSPTS+GGGD+ TRI AR+S IESFKGCG+TGMRIDKE+LRK+LRMP+YLR AMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPGSGTVSGS------TDGPPESPVVVFINPRSGGRHGHLLKERLQMLIS 120
           LQDP AGESRLPG+ ++SGS       DGPPESP+VVFINPRSGGRHG LLKERLQMLIS
Sbjct: 61  LQDPGAGESRLPGASSISGSPFAQDTDDGPPESPMVVFINPRSGGRHGRLLKERLQMLIS 120

Query: 121 DEQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180
           +EQVFDLTDVKPHEFV+YGLRCLEL A LGDACAKETREKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLDSW 240
           ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG IR+LDSW
Sbjct: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLDSW 240

Query: 241 HVILSAPSSENVELQLPHCMKPTEALALDESLEIEEASSEKASCHQGVFYNYFSIGMDAQ 300
            V LS+PS EN++  LPHCMKPTE  ALDESLEIEEA  EKA+CH+GVFYNYFSIGMDAQ
Sbjct: 241 QVFLSSPSGENMD--LPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFSIGMDAQ 300

Query: 301 VAYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKKV 360
           VAYGFHHLRNEKPYLAQGPI+NKLIYSGYSCGQGWFFT C S+PSLRGLKNILRMHVKK+
Sbjct: 301 VAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILRMHVKKI 360

Query: 361 NCSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFGL 420
           NCSDWE + IPSSVRSLVALNLNNYGSGRHPWG+LSPEYMEK+GFV AHVDDGLLEVFGL
Sbjct: 361 NCSDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGLLEVFGL 420

Query: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFVEI 480
           KQGWHASLVMGELISAKHIVQAAAIRFELRGGEW++ FLQMDGEPWKQPM++EFSTFVEI
Sbjct: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEFSTFVEI 480

Query: 481 KRVPSQSLMINGE 488
           KRVP QSLMI+GE
Sbjct: 481 KRVPCQSLMISGE 491

BLAST of Sed0001255 vs. ExPASy TrEMBL
Match: A0A6J1KDS8 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111492334 PE=3 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.8e-255
Identity = 428/493 (86.82%), Postives = 460/493 (93.31%), Query Frame = 0

Query: 1   MDSPTSKGGGDAKTRIAARTSTIESFKGCGVTGMRIDKEELRKKLRMPEYLRLAMRDSIR 60
           MDSPTS+GGGD+ TRI AR+S IESFKGCG+TGMRIDKE+LRK+LRMP+YLR AMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPGSGTVSGS------TDGPPESPVVVFINPRSGGRHGHLLKERLQMLIS 120
           LQDP AGESRLPG+ ++SGS       DGPPESP+VVFINP+SGGRHG LLKERLQMLIS
Sbjct: 61  LQDPGAGESRLPGASSISGSPFAQDTDDGPPESPMVVFINPQSGGRHGRLLKERLQMLIS 120

Query: 121 DEQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180
           +EQVFD+TDVKPHEFV+YGLRCLEL A LGDACAKETREKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDITDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLDSW 240
           ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG IR+LDSW
Sbjct: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLDSW 240

Query: 241 HVILSAPSSENVELQLPHCMKPTEALALDESLEIEEASSEKASCHQGVFYNYFSIGMDAQ 300
            V LS+PS EN++  LPHCMKPTE  ALDESLEIEEA  EKA+CH+GVFYNYFSIGMDAQ
Sbjct: 241 QVFLSSPSGENMD--LPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFSIGMDAQ 300

Query: 301 VAYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKKV 360
           VAYGFHHLRNEKPYLAQGPI+NKLIYSGYSCGQGWFFT C S+PSLRGLKNILRMHVKK+
Sbjct: 301 VAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILRMHVKKI 360

Query: 361 NCSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFGL 420
           NCSDWE + IPSSVRSLVALNLNNYGSGRHPWG+LSPEYMEK+GFV AHVDDGLLEVFGL
Sbjct: 361 NCSDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGLLEVFGL 420

Query: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFVEI 480
           KQGWHASLVMGELISAKHIVQAAAIRFELRGGEW++ FLQMDGEPWKQPM++EFSTFVEI
Sbjct: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEFSTFVEI 480

Query: 481 KRVPSQSLMINGE 488
           KRVP QSLMI+GE
Sbjct: 481 KRVPCQSLMISGE 491

BLAST of Sed0001255 vs. ExPASy TrEMBL
Match: A0A1S3BRW0 (diacylglycerol kinase OS=Cucumis melo OX=3656 GN=LOC103493013 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.5e-254
Identity = 431/495 (87.07%), Postives = 458/495 (92.53%), Query Frame = 0

Query: 1   MDSPTSKGGGDAKTRIAARTSTIESFKGCGVTGMRIDKEELRKKLRMPEYLRLAMRDSIR 60
           MDSPTS+GGGD+ TRIAAR+S IESFKGCG+TG+RIDKE+LRKKL+MP+YLR AMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEDLRKKLQMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPG--------SGTVSGSTDGPPESPVVVFINPRSGGRHGHLLKERLQML 120
           LQDP+AGESRLPG        S  V  ST+ PPESP+VVFINPRSGGRHG LLK+RLQML
Sbjct: 61  LQDPSAGESRLPGTRLDSLSASTFVPESTEEPPESPMVVFINPRSGGRHGRLLKDRLQML 120

Query: 121 ISDEQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGC 180
           IS+EQVFDLTDVKPHEFV+YGL CLEL AELGDACAK+ R+K+RVMVAGGDGTVGWVLGC
Sbjct: 121 ISEEQVFDLTDVKPHEFVRYGLGCLELLAELGDACAKDVRQKIRVMVAGGDGTVGWVLGC 180

Query: 181 LLELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLD 240
           LLELEKK R PVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG IRKLD
Sbjct: 181 LLELEKKDRRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRKLD 240

Query: 241 SWHVILSAPSSENVELQLPHCMKPTEALALDESLEIEEASSEKASCHQGVFYNYFSIGMD 300
           SWHV LS PS ENV  QLPHCMKPTE LALDESLEIEEA SEKASCH+GVFYNYFSIGMD
Sbjct: 241 SWHVFLSTPSGENV--QLPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFSIGMD 300

Query: 301 AQVAYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVK 360
           AQVAYGFH+LRNEKPYLAQGPI+NKLIYSGYSCGQGWFFT C S+P LRGLKNILRMHVK
Sbjct: 301 AQVAYGFHNLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVK 360

Query: 361 KVNCSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVF 420
           KVNCSDWEQ++IPSSVRSLVALNLNNYGSGRHPWG+L+PEYMEK+GFV A VDDGLLEVF
Sbjct: 361 KVNCSDWEQILIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVF 420

Query: 421 GLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFV 480
           GLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKD FLQMDGEPWKQPMSNEFSTFV
Sbjct: 421 GLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEFSTFV 480

Query: 481 EIKRVPSQSLMINGE 488
           EIKRVP QSLMI+GE
Sbjct: 481 EIKRVPCQSLMISGE 493

BLAST of Sed0001255 vs. ExPASy TrEMBL
Match: A0A0A0LK87 (diacylglycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_2G058110 PE=3 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 5.8e-251
Identity = 426/495 (86.06%), Postives = 455/495 (91.92%), Query Frame = 0

Query: 1   MDSPTSKGGGDAKTRIAARTSTIESFKGCGVTGMRIDKEELRKKLRMPEYLRLAMRDSIR 60
           MDSPTS+GG D+ TRIA+R+S IESFKGCG+TG+RIDKE+LRKKL+MP+YLR AMRDSIR
Sbjct: 1   MDSPTSRGGDDSTTRIASRSSMIESFKGCGLTGLRIDKEDLRKKLQMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPG--------SGTVSGSTDGPPESPVVVFINPRSGGRHGHLLKERLQML 120
           LQDP AGESRLPG        S +V  +T+ PP+SP+VVFINPRSGGRHG LLK+RLQML
Sbjct: 61  LQDPTAGESRLPGTRLDSISASTSVPETTEQPPDSPMVVFINPRSGGRHGRLLKDRLQML 120

Query: 121 ISDEQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGC 180
           IS+EQVFDLTDVKPHEFV+YGL CLEL AELGDACAK+ REKMRVMVAGGDGTVGWVLGC
Sbjct: 121 ISEEQVFDLTDVKPHEFVRYGLGCLELLAELGDACAKDVREKMRVMVAGGDGTVGWVLGC 180

Query: 181 LLELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLD 240
           LLELEKK R PVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG IRKLD
Sbjct: 181 LLELEKKDRRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRKLD 240

Query: 241 SWHVILSAPSSENVELQLPHCMKPTEALALDESLEIEEASSEKASCHQGVFYNYFSIGMD 300
           SWHV LS PS EN   +LPHCMKPTE LALDESLEIEEA SEKASC++GVFYNYFSIGMD
Sbjct: 241 SWHVFLSTPSGENG--KLPHCMKPTEELALDESLEIEEALSEKASCYEGVFYNYFSIGMD 300

Query: 301 AQVAYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVK 360
           AQVAYGFH+LRNEKPYLAQGPI+NKLIYSGYSCGQGWFFT C S+P LRGLKNILRMHVK
Sbjct: 301 AQVAYGFHNLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVK 360

Query: 361 KVNCSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVF 420
           KVNCSDWEQV+IPSSVRSLVALNLNNYGSGRHPWG+L+PEYMEK+GFV A VDDGLLEVF
Sbjct: 361 KVNCSDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVF 420

Query: 421 GLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFV 480
           GLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKD FLQMDGEPWKQ MSNEFSTFV
Sbjct: 421 GLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDSFLQMDGEPWKQSMSNEFSTFV 480

Query: 481 EIKRVPSQSLMINGE 488
           EIKRVP QSLMI+GE
Sbjct: 481 EIKRVPCQSLMISGE 493

BLAST of Sed0001255 vs. ExPASy TrEMBL
Match: A0A6J1DIK2 (diacylglycerol kinase OS=Momordica charantia OX=3673 GN=LOC111020846 PE=3 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 1.7e-250
Identity = 424/492 (86.18%), Postives = 457/492 (92.89%), Query Frame = 0

Query: 1   MDSPTSKGGGDAKTRIAARTSTIESFKGCGVTGMRIDKEELRKKLRMPEYLRLAMRDSIR 60
           M+S T+ GG D+ TRIAAR+S IES KGCG+TG+ +DKEELRKKL+MP+YLR AMRDSIR
Sbjct: 1   MESLTTNGGEDSSTRIAARSSMIESLKGCGLTGIHVDKEELRKKLQMPQYLRFAMRDSIR 60

Query: 61  LQDPAAGESRLPGSGTVSGST-----DGPPESPVVVFINPRSGGRHGHLLKERLQMLISD 120
           LQDPAAGESRLP + ++SGS      + PPESP+VVFINPRSGGRHG LLKERLQMLIS+
Sbjct: 61  LQDPAAGESRLPAANSLSGSAFAPEINEPPESPMVVFINPRSGGRHGRLLKERLQMLISE 120

Query: 121 EQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGCLLE 180
           EQVFDLTDVKPHEFVKYGL+CLEL A +GDACAKETR+KMRVMVAGGDGTVGWVLGCLLE
Sbjct: 121 EQVFDLTDVKPHEFVKYGLKCLELLAAIGDACAKETRDKMRVMVAGGDGTVGWVLGCLLE 180

Query: 181 LEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLDSWH 240
           LEK+GRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTG + KLDSW+
Sbjct: 181 LEKQGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGRVCKLDSWN 240

Query: 241 VILSAPSSENVELQLPHCMKPTEALALDESLEIEEASSEKASCHQGVFYNYFSIGMDAQV 300
           V+LSAP+S NVE  LPHCM+PTE  ALDES EI+EASSEKASCH GVFYNYFSIGMDAQV
Sbjct: 241 VLLSAPNSINVE--LPHCMRPTEDGALDESFEIKEASSEKASCHGGVFYNYFSIGMDAQV 300

Query: 301 AYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKKVN 360
           AYGFHHLRNEKPYLAQGPI+NKLIYSGYSCGQGWFFT C S+P LRGLKNILRMHVKKVN
Sbjct: 301 AYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVN 360

Query: 361 CSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFGLK 420
           CSDWEQV+IPSSVRSLVALNLNNYGSGRHPWG+L+PEYMEK+GFV A VDDGLLEVFGLK
Sbjct: 361 CSDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLK 420

Query: 421 QGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFVEIK 480
           QGWHASLVMGELISAKHIVQAAAIRFELRGGEWK+ FLQMDGEPWKQPMS+EFSTFVEIK
Sbjct: 421 QGWHASLVMGELISAKHIVQAAAIRFELRGGEWKNAFLQMDGEPWKQPMSDEFSTFVEIK 480

Query: 481 RVPSQSLMINGE 488
           RVPSQSLMINGE
Sbjct: 481 RVPSQSLMINGE 490

BLAST of Sed0001255 vs. TAIR 10
Match: AT2G18730.1 (diacylglycerol kinase 3 )

HSP 1 Score: 672.2 bits (1733), Expect = 3.2e-193
Identity = 318/494 (64.37%), Postives = 388/494 (78.54%), Query Frame = 0

Query: 1   MDSPTSKGGGDAKTRIAARTSTIES--FKGCGVTGMR---IDKEELRKKLRMPEYLRLAM 60
           MDSP SK     +  +A+R ST +S   +GCG+  +    +DK ELR++L MPEYLRLAM
Sbjct: 1   MDSPVSKTDASKEKFVASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAM 60

Query: 61  RDSIRLQDPAA--GESRLPGSGTVSGSTDGPPESPVVVFINPRSGGRHGHLLKERLQMLI 120
           RD I+ +D +A      LPG        D  P +P+VVFINP SGGRHG +LKERLQ L+
Sbjct: 61  RDCIKRKDSSAIPDHLLLPGGAV----ADMAPHAPMVVFINPNSGGRHGPVLKERLQQLM 120

Query: 121 SDEQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGCL 180
           S+EQVFDLT+VKPHEFV+YGL CLE  A  GD CAKE R ++R+MVAGGDGTVGWVLGCL
Sbjct: 121 SEEQVFDLTEVKPHEFVRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCL 180

Query: 181 LELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLDS 240
            EL K G+  +PPVGVIPLGTGNDLSR+FGWGGSFPFAW+SA+KR+L RA+ GP+ +LDS
Sbjct: 181 GELNKDGKSQIPPVGVIPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDS 240

Query: 241 WHVILSAPSSENVELQLPHCMKPTEALALDESLEIEEASSEKASCHQGVFYNYFSIGMDA 300
           W +++S PS E V+   P+ +KP E   LD+ L+    +   A  ++GVFYNY SIGMDA
Sbjct: 241 WKILVSMPSGEVVD--PPYSLKPAEENELDQGLDAGIDAPPLAKAYEGVFYNYLSIGMDA 300

Query: 301 QVAYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKK 360
           QVAYGFHHLRN KPYLAQGPISNK+IYS + C QGWF T C ++P LRGL+NI+++H+KK
Sbjct: 301 QVAYGFHHLRNTKPYLAQGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKK 360

Query: 361 VNCSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFG 420
           VNCS WE++ +P +VRS+VALNL++YGSG HPWG+L P+Y+EK+GFV AH DDGL+E+FG
Sbjct: 361 VNCSQWEEIAVPKNVRSIVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFG 420

Query: 421 LKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFVE 480
            KQGWHAS VM ELISAKHI QAAA+RFELRGG+W+D FLQMDGEPWKQPMS E+STFVE
Sbjct: 421 FKQGWHASFVMAELISAKHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVE 480

Query: 481 IKRVPSQSLMINGE 488
           IK+VP QSLMIN E
Sbjct: 481 IKKVPYQSLMINNE 488

BLAST of Sed0001255 vs. TAIR 10
Match: AT4G30340.1 (diacylglycerol kinase 7 )

HSP 1 Score: 671.0 bits (1730), Expect = 7.2e-193
Identity = 320/495 (64.65%), Postives = 393/495 (79.39%), Query Frame = 0

Query: 5   TSKGGGDAKT------RIAARTSTIESFKGCGVTGMR---IDKEELRKKLRMPEYLRLAM 64
           T +  G+A T      R +A+T    + +GCG   +    IDKEELR +L MPEYLR+AM
Sbjct: 4   TPRSVGEASTTNFVAARPSAKTDDAVTMRGCGFANLALVGIDKEELRGRLAMPEYLRIAM 63

Query: 65  RDSIRLQDPA--AGESRLPGSGTVSGSTDGPPESPVVVFINPRSGGRHGHLLKERLQMLI 124
           RD I+ +D    +    LPG      + D  P +P+VVFINP+SGGRHG +LKERLQ L+
Sbjct: 64  RDCIKRKDSTEISDHLLLPG----GAAADMAPHAPMVVFINPKSGGRHGPVLKERLQQLM 123

Query: 125 SDEQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGCL 184
           ++EQVFDLT+VKPHEFV+YGL CL+  A  GD CA+E REK+R+MVAGGDGTVGWVLGCL
Sbjct: 124 TEEQVFDLTEVKPHEFVRYGLGCLDTLAAKGDECARECREKIRIMVAGGDGTVGWVLGCL 183

Query: 185 LELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLDS 244
            EL K G+  +PPVGVIPLGTGNDLSR+F WGGSFPFAW+SA+KR+L RAT G I +LDS
Sbjct: 184 GELHKDGKSHIPPVGVIPLGTGNDLSRSFSWGGSFPFAWRSAMKRTLHRATLGSIARLDS 243

Query: 245 WHVILSAPSSENVELQLPHCMKPT-EALALDESLEIEEASSEKASCHQGVFYNYFSIGMD 304
           W +++S PS E V+   P+ +KPT E  ALD++L+ +     KA  ++GVFYNYFSIGMD
Sbjct: 244 WKIVVSMPSGEVVD--PPYSLKPTIEETALDQALDADGDVPPKAKSYEGVFYNYFSIGMD 303

Query: 305 AQVAYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVK 364
           AQVAYGFHHLRNEKPYLAQGP++NK+IYS YSC QGWF T C +NP+LRGL+NI+++H+K
Sbjct: 304 AQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYSCTQGWFCTPCVNNPALRGLRNIMKIHIK 363

Query: 365 KVNCSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVF 424
           K NCS+WE++ +P SVRS+V LNL NYGSGRHPWG+L P+Y+EK+GFV AH DDGL+E+F
Sbjct: 364 KANCSEWEEIHVPKSVRSIVVLNLYNYGSGRHPWGNLRPKYLEKRGFVEAHCDDGLIEIF 423

Query: 425 GLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFV 484
           GLKQGWHAS VM E+ISAKHI QAAAIRFELRGG+WK+ FLQMDGEPWKQPM +++STFV
Sbjct: 424 GLKQGWHASFVMAEIISAKHIAQAAAIRFELRGGDWKNAFLQMDGEPWKQPMKSDYSTFV 483

Query: 485 EIKRVPSQSLMINGE 488
           EIK+VP QSLMINGE
Sbjct: 484 EIKKVPFQSLMINGE 492

BLAST of Sed0001255 vs. TAIR 10
Match: AT5G57690.1 (diacylglycerol kinase 4 )

HSP 1 Score: 636.0 bits (1639), Expect = 2.6e-182
Identity = 299/493 (60.65%), Postives = 386/493 (78.30%), Query Frame = 0

Query: 1   MDSPTSKGGGDAKTRIAARTSTIESFKGCGVT------GMRIDKEELRKKLRMPEYLRLA 60
           M+SP+   G     R+  R S+++SF    V+       +R+ K ELR+++ +P+YLR+A
Sbjct: 1   MESPSI--GDSLTARMIPRHSSLDSFGAMKVSLLVNLASIRVSKAELRQRVMLPQYLRIA 60

Query: 61  MRDSIRLQDPAAGESRLPGSGTVSGSTDGPPESPVVVFINPRSGGRHGHLLKERLQMLIS 120
           +RD I  +D +   S        + +    PE P++VF+NP+SGGR G L+KERLQ LIS
Sbjct: 61  IRDCILRKDDSFDASSSVAPPLENNAL--TPEVPLMVFVNPKSGGRQGPLIKERLQNLIS 120

Query: 121 DEQVFDLTDVKPHEFVKYGLRCLELFAELGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180
           +EQV+DLT+VKP+EF++YGL CLE FA  GD CAKE REKMR++VAGGDGTVGWVLGCL 
Sbjct: 121 EEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIREKMRIVVAGGDGTVGWVLGCLG 180

Query: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGPIRKLDSW 240
           EL  + RLPVPPV ++PLGTGNDLSR+FGWGGSFPFAWKSAIKR+L RA+  PI +LDSW
Sbjct: 181 ELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAWKSAIKRTLHRASVAPISRLDSW 240

Query: 241 HVILSAPSSENVELQLPHCMKPTEALALDESLEIEEASSEKASCHQGVFYNYFSIGMDAQ 300
           +++++ PS E V+   P+ +K T+   +D++LEIE       + ++GVFYNYFSIGMDAQ
Sbjct: 241 NILITMPSGEIVD--PPYSLKATQECYIDQNLEIEGEIPPSTNGYEGVFYNYFSIGMDAQ 300

Query: 301 VAYGFHHLRNEKPYLAQGPISNKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKKV 360
           VAYGFHHLRNEKPYLA GPI+NK+IYSGY C QGWF T C ++P LRGLKNI+ +H+KK+
Sbjct: 301 VAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFLTHCINDPGLRGLKNIMTLHIKKL 360

Query: 361 NCSDWEQVVIPSSVRSLVALNLNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFGL 420
           + S+WE+V +P SVR++VALNL++YGSGR+PWG+L  +Y+EK+GFV A  DDGLLE+FGL
Sbjct: 361 DSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDYLEKRGFVEAQADDGLLEIFGL 420

Query: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDGFLQMDGEPWKQPMSNEFSTFVEI 480
           KQGWHAS VM ELISAKHI QAAAIR E+RGG+WKD F+QMDGEPWKQPM+ ++STFV+I
Sbjct: 421 KQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDAFMQMDGEPWKQPMTRDYSTFVDI 480

Query: 481 KRVPSQSLMINGE 488
           KRVP QSL++ G+
Sbjct: 481 KRVPHQSLVVKGD 487

BLAST of Sed0001255 vs. TAIR 10
Match: AT2G20900.1 (diacylglycerol kinase 5 )

HSP 1 Score: 268.9 bits (686), Expect = 8.2e-72
Identity = 141/409 (34.47%), Postives = 223/409 (54.52%), Query Frame = 0

Query: 85  PESPVVVFINPRSGGRHGHLLKERLQMLISDEQVFDLTDVKPHEFVKYGLRCLELFAELG 144
           P SPV+VFIN +SGG+ G  L    + L++  QVFDL    P + ++     LE   +  
Sbjct: 37  PASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLKD-- 96

Query: 145 DACAKETREKMRVMVAGGDGTVGWVLGCLLELEKKGRLPVPPVGVIPLGTGNDLSRTFGW 204
           D  A++ REK++++VAGGDGT GW+LG + +L+       PP+  +PLGTGN+L   FGW
Sbjct: 97  DDFARQIREKLKIIVAGGDGTAGWLLGVVCDLKLS---HPPPIATVPLGTGNNLPFAFGW 156

Query: 205 GGSFPFAWKSAIKRSLDRATTGPIRKLDSWHVILSAPSSEN-------VELQLPHCMKPT 264
           G   P   ++A++  L++     + K+D+WH+++   + +          L+LPH +   
Sbjct: 157 GKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPHSLHAF 216

Query: 265 EALALDESLEIEEASSEKASCH--QGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPIS 324
             ++  + L        K  CH  +G F+NYFS+GMDAQ++Y FH  R   P   +  + 
Sbjct: 217 HRVSPTDEL-------NKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFKNQLV 276

Query: 325 NKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKKVNCSDWEQVVIPSSVRSLVALN 384
           N+  Y    C QGWF  S F +P+ R +  + ++ +   N   W+ + IP S+RS+V LN
Sbjct: 277 NQSTYVKLGCTQGWFCASLF-HPASRNIAQLAKVKIATRN-GQWQDLHIPHSIRSIVCLN 336

Query: 385 LNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFGLKQGWHASLVMGELISAKHIVQ 444
           L ++  G +PWG  +P     +G     VDDGL+EV G +  WH  +++        + Q
Sbjct: 337 LPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGTRLAQ 396

Query: 445 AAAIRFELRGGEWKDGFLQMDGEPWKQPMS-NEFSTFVEIKRVPSQSLM 484
           A  IRFE   G     F++MDGEPWKQP+  ++ +  VEI  +   +++
Sbjct: 397 ANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNML 431

BLAST of Sed0001255 vs. TAIR 10
Match: AT2G20900.3 (diacylglycerol kinase 5 )

HSP 1 Score: 268.9 bits (686), Expect = 8.2e-72
Identity = 141/409 (34.47%), Postives = 223/409 (54.52%), Query Frame = 0

Query: 85  PESPVVVFINPRSGGRHGHLLKERLQMLISDEQVFDLTDVKPHEFVKYGLRCLELFAELG 144
           P SPV+VFIN +SGG+ G  L    + L++  QVFDL    P + ++     LE   +  
Sbjct: 37  PASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLKD-- 96

Query: 145 DACAKETREKMRVMVAGGDGTVGWVLGCLLELEKKGRLPVPPVGVIPLGTGNDLSRTFGW 204
           D  A++ REK++++VAGGDGT GW+LG + +L+       PP+  +PLGTGN+L   FGW
Sbjct: 97  DDFARQIREKLKIIVAGGDGTAGWLLGVVCDLKLS---HPPPIATVPLGTGNNLPFAFGW 156

Query: 205 GGSFPFAWKSAIKRSLDRATTGPIRKLDSWHVILSAPSSEN-------VELQLPHCMKPT 264
           G   P   ++A++  L++     + K+D+WH+++   + +          L+LPH +   
Sbjct: 157 GKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPHSLHAF 216

Query: 265 EALALDESLEIEEASSEKASCH--QGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPIS 324
             ++  + L        K  CH  +G F+NYFS+GMDAQ++Y FH  R   P   +  + 
Sbjct: 217 HRVSPTDEL-------NKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFKNQLV 276

Query: 325 NKLIYSGYSCGQGWFFTSCFSNPSLRGLKNILRMHVKKVNCSDWEQVVIPSSVRSLVALN 384
           N+  Y    C QGWF  S F +P+ R +  + ++ +   N   W+ + IP S+RS+V LN
Sbjct: 277 NQSTYVKLGCTQGWFCASLF-HPASRNIAQLAKVKIATRN-GQWQDLHIPHSIRSIVCLN 336

Query: 385 LNNYGSGRHPWGDLSPEYMEKKGFVAAHVDDGLLEVFGLKQGWHASLVMGELISAKHIVQ 444
           L ++  G +PWG  +P     +G     VDDGL+EV G +  WH  +++        + Q
Sbjct: 337 LPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGTRLAQ 396

Query: 445 AAAIRFELRGGEWKDGFLQMDGEPWKQPMS-NEFSTFVEIKRVPSQSLM 484
           A  IRFE   G     F++MDGEPWKQP+  ++ +  VEI  +   +++
Sbjct: 397 ANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNML 431