Sed0001140 (gene) Chayote v1

BLAST of Sed0001140 vs. NCBI nr
Match: XP_023520714.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 578.6 bits (1490), Expect = 4.0e-161
Identity = 294/330 (89.09%), Postives = 316/330 (95.76%), Query Frame = 0

Query: 41  MEAVEDDHCAKTEPPCSPSGCNPPEPPMSKNARKRLLKQQRFEAKKAEKKALEKEQKKKN 100
           MEA+E+D  AK +PPCSPSGCNPPEPP+SKNARK+LLKQQR+EAKKAEKKA EKE KKK+
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKELKKKD 60

Query: 101 IERKRKEWEDTLASVTEEERSKLIESRKNLRKERMGKRTEEKEKKMERLNKARDCGQNIV 160
           IERKRKEWE+TLASVTEEER+KLIESRK+LRKERMGKR+EEKEKK+ERLN+A+DCGQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 161 IDLEFSHLMTPSEIHSLVQQIMYCYAMNGRCPSPGHLWLTGCNGEMETQLQKIPGFDKWI 220
           IDLEFS LMTPSEIHSLVQQIMYCYA+NGRCPSPGHLWLTGCNGEMETQLQ++PGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 221 IEKEHGSYIDALQDQKENLVYLTADAETVLDDLDLKKTYIIGGLVDRNRWKGITMKKAQE 280
           IEKEH SYIDALQDQKENLVYLTAD+ETVLDDLDLKK YIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 281 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCEADSNS 340
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWK SFFQVVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 341 DHAVDGEENGYGDDEHETKKTKCDEISSSN 371
           DHAVDGEENG  DDE ETKKTKCDE+SSSN
Sbjct: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSN 330

BLAST of Sed0001140 vs. NCBI nr
Match: KAG6583717.1 (hypothetical protein SDJN03_19649, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 577.8 bits (1488), Expect = 6.8e-161
Identity = 294/330 (89.09%), Postives = 316/330 (95.76%), Query Frame = 0

Query: 41  MEAVEDDHCAKTEPPCSPSGCNPPEPPMSKNARKRLLKQQRFEAKKAEKKALEKEQKKKN 100
           MEA E+D  AK +PPCSPSGCNPPEPP+SKNARK+LLKQQR+EAKKAEKKA EKEQKKK+
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 101 IERKRKEWEDTLASVTEEERSKLIESRKNLRKERMGKRTEEKEKKMERLNKARDCGQNIV 160
           IERKRKEWE+TLASVTEEER+KLIESRK+LRKERMGKR+EEKEKK+ERLN+A+DCGQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 161 IDLEFSHLMTPSEIHSLVQQIMYCYAMNGRCPSPGHLWLTGCNGEMETQLQKIPGFDKWI 220
           IDLEFS LMTPSEIHSLVQQIMYCYA+NGRCPSPGHLWLTGCNGEMETQLQ++PGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 221 IEKEHGSYIDALQDQKENLVYLTADAETVLDDLDLKKTYIIGGLVDRNRWKGITMKKAQE 280
           IE+EH SYIDALQDQKENLVYLTAD+ETVLDDLDLKK YIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEREHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 281 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCEADSNS 340
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWK SFFQVVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 341 DHAVDGEENGYGDDEHETKKTKCDEISSSN 371
           DHAVDGEENG  DDE ETKKTKCDE+SSSN
Sbjct: 301 DHAVDGEENGDRDDEQETKKTKCDEVSSSN 330

BLAST of Sed0001140 vs. NCBI nr
Match: XP_022973151.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita maxima])

HSP 1 Score: 574.7 bits (1480), Expect = 5.7e-160
Identity = 291/330 (88.18%), Postives = 315/330 (95.45%), Query Frame = 0

Query: 41  MEAVEDDHCAKTEPPCSPSGCNPPEPPMSKNARKRLLKQQRFEAKKAEKKALEKEQKKKN 100
           MEA+E+D  AK +PPCSPSGCNPPEPP+SKNARK+LLKQQR+EAKKAEKKA EKEQKKK+
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 101 IERKRKEWEDTLASVTEEERSKLIESRKNLRKERMGKRTEEKEKKMERLNKARDCGQNIV 160
           IERKRKEWEDTLASVTEEER+KLIESR++LRKERMGKR+EEKEKK+ERLN+A+DCGQ I+
Sbjct: 61  IERKRKEWEDTLASVTEEERAKLIESRRSLRKERMGKRSEEKEKKIERLNRAKDCGQKII 120

Query: 161 IDLEFSHLMTPSEIHSLVQQIMYCYAMNGRCPSPGHLWLTGCNGEMETQLQKIPGFDKWI 220
           IDLEFS LMTPSEIHSLVQQIMYCYA+NGRCPSPGHLWLTGCNGEMETQLQ++PGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 221 IEKEHGSYIDALQDQKENLVYLTADAETVLDDLDLKKTYIIGGLVDRNRWKGITMKKAQE 280
           IEKEH SYIDALQDQKENLVYLTAD+ETVLDDLDLKK YIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 281 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCEADSNS 340
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWK SFFQVVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 341 DHAVDGEENGYGDDEHETKKTKCDEISSSN 371
           DHAVDGEENG  DDE E+KK KC E+SSSN
Sbjct: 301 DHAVDGEENGDRDDEQESKKIKCGEVSSSN 330

BLAST of Sed0001140 vs. NCBI nr
Match: XP_022927615.1 (tRNA (guanine(9)-N1)-methyltransferase [Cucurbita moschata])

HSP 1 Score: 572.4 bits (1474), Expect = 2.8e-159
Identity = 291/330 (88.18%), Postives = 314/330 (95.15%), Query Frame = 0

Query: 41  MEAVEDDHCAKTEPPCSPSGCNPPEPPMSKNARKRLLKQQRFEAKKAEKKALEKEQKKKN 100
           MEA E+D  AK +PPCSPSGCNPPEPP+SKNARK+LLKQQR+EAKKAEKKA EKEQKKK+
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 101 IERKRKEWEDTLASVTEEERSKLIESRKNLRKERMGKRTEEKEKKMERLNKARDCGQNIV 160
           IERKRKEWE+TLASVTEEER+KLIESRK+LRKERMGKR+EEKEKK+ERLN+A+DCGQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 161 IDLEFSHLMTPSEIHSLVQQIMYCYAMNGRCPSPGHLWLTGCNGEMETQLQKIPGFDKWI 220
           IDLEFS LMTPSEIHSLVQQIMYCYA+NGRCPSPGHLWLTGCNGEMETQLQ++PGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 221 IEKEHGSYIDALQDQKENLVYLTADAETVLDDLDLKKTYIIGGLVDRNRWKGITMKKAQE 280
           IEKEH SYIDALQD KENLVYLTAD+ETVLDDLDLKK YIIGGLVDRNRWKGITM KAQE
Sbjct: 181 IEKEHRSYIDALQDHKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMNKAQE 240

Query: 281 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCEADSNS 340
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILL+Y+ETRDWK SFFQVVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLRYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 341 DHAVDGEENGYGDDEHETKKTKCDEISSSN 371
           DHAVDG+ENG  DDE ETKKTKCDE+SSSN
Sbjct: 301 DHAVDGKENGDRDDEQETKKTKCDEVSSSN 330

BLAST of Sed0001140 vs. NCBI nr
Match: XP_038893739.1 (tRNA (guanine(9)-N1)-methyltransferase [Benincasa hispida])

HSP 1 Score: 558.9 bits (1439), Expect = 3.3e-155
Identity = 291/334 (87.13%), Postives = 310/334 (92.81%), Query Frame = 0

Query: 41  MEAVEDDHCAKTEPPCSPSGCNPPEPPMSKNARKRLLKQQRFEAKKAEKKALEKEQKKKN 100
           MEAVE+D  AK +PP SP   NPPEPP+SKNA+K+LLKQQR+EAKKAEKK   KEQKKK+
Sbjct: 1   MEAVEEDQSAKMDPPPSPFAFNPPEPPLSKNAKKKLLKQQRYEAKKAEKKLQAKEQKKKD 60

Query: 101 IERKRKEWEDTLASVTEEERSKLIESRKNLRKERMGKRTEEKEKKMERLNKARDCGQNIV 160
           IERKRKEWEDTLASVTEEERSKLIESR++LRKERMGKR+EE+EKK+ERLN+A+DCGQNIV
Sbjct: 61  IERKRKEWEDTLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNRAKDCGQNIV 120

Query: 161 IDLEFSHLMTPSEIHSLVQQIMYCYAMNGRCPSPGHLWLTGCNGEMETQLQKIPGFDKWI 220
           IDLEFSHLMTPSEIHSLVQQIMYCYA+NGRCPSPGHLWLTGCNGEMETQLQ++PGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 221 IEKEHGSYIDALQDQKENLVYLTADAETVLDDLDLKKTYIIGGLVDRNRWKGITMKKAQE 280
           IEKEH SYIDALQDQKENLVYLTADAETVLDDLD KK YIIGGLVDRNRWKGITMKKA E
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDPKKIYIIGGLVDRNRWKGITMKKAHE 240

Query: 281 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCEADSNS 340
           QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWK SFFQVVPQRKRC ADSNS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFQVVPQRKRCGADSNS 300

Query: 341 DHAVDGEEN----GYGDDEHETKKTKCDEISSSN 371
           DH VD EEN    G GDDEHE KKTKCDE+SSS+
Sbjct: 301 DHPVDEEENGDGDGDGDDEHEMKKTKCDEVSSSD 334

BLAST of Sed0001140 vs. ExPASy Swiss-Prot
Match: Q8TBZ6 (tRNA methyltransferase 10 homolog A OS=Homo sapiens OX=9606 GN=TRMT10A PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.9e-36
Identity = 116/343 (33.82%), Postives = 192/343 (55.98%), Query Frame = 0

Query: 40  KMEAVEDDHCAKTEPPCSPSGCNPPEPPMSKNARKRLLKQQRFEAKKAEKKALEKEQKKK 99
           K + + +D   +++ P    GC     P+SK   K+L+KQ+++E    E++ L K+++K+
Sbjct: 17  KKQGINEDQ-EESQKPRLGEGCE----PISKRQMKKLIKQKQWE----EQRELRKQKRKE 76

Query: 100 NIERKRKEWEDTLASVTEEERSKLIESRKNLRKERMGKRTEEKEKKMERLNKARDCGQNI 159
             +RKRK+ E                     R+ +M   ++  ++K  R +        +
Sbjct: 77  --KRKRKKLE---------------------RQCQMEPNSDGHDRKRVRRDVVHST-LRL 136

Query: 160 VIDLEFSHLMTPSEIHSLVQQIMYCYAMNGRCPSPGHLWLTGCNGEMETQL-QKIPGFDK 219
           +ID  F HLM   +I  L +QI  CYA N R   P   +LT   G+++  + +   G+  
Sbjct: 137 IIDCSFDHLMVLKDIKKLHKQIQRCYAENRRALHPVQFYLTSHGGQLKKNMDENDKGWVN 196

Query: 220 W---IIEKEHGSYIDALQDQKENLVYLTADAETVLDDLDLKKTYIIGGLVDRNRWKGITM 279
           W    I+ EH S +     +KE+L+YLT+D+  +L +LD  K Y+IGGLVD N  KG+T 
Sbjct: 197 WKDIHIKPEHYSEL----IKKEDLIYLTSDSPNILKELDESKAYVIGGLVDHNHHKGLTY 256

Query: 280 KKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQR---- 339
           K+A + GI  A+LP+G+++KM+S +VL VN V EI+L+YLETRDW+++FF ++PQR    
Sbjct: 257 KQASDYGINHAQLPLGNFVKMNSRKVLAVNHVFEIILEYLETRDWQEAFFTILPQRKGAV 316

Query: 340 ---KRCEADSNSDHAVDGEENGYGDDEHETKKTKCDEISSSNQ 372
              K CE+ S+ + +V  EE G   D  E + ++ +E+ S ++
Sbjct: 317 PTDKACESASHDNQSVRMEEGGSDSDSSEEEYSR-NELDSPHE 321

BLAST of Sed0001140 vs. ExPASy Swiss-Prot
Match: Q8C1Z8 (tRNA methyltransferase 10 homolog A OS=Mus musculus OX=10090 GN=Trmt10a PE=2 SV=2)

HSP 1 Score: 153.7 bits (387), Expect = 4.1e-36
Identity = 105/306 (34.31%), Postives = 168/306 (54.90%), Query Frame = 0

Query: 31  PSAQKSPNPKMEAVEDDHCAKTEPPCSPSGCNPPEPPMSKNARKRLLKQQRFEAKKAEKK 90
           P++ +SPN + +    D   + + P   +G      P+SK   K+L+KQ+++E ++ ++K
Sbjct: 7   PASIESPNVEEKLGTSDGEEERQEPRVDAGAE----PISKRQLKKLMKQKQWEEQREQRK 66

Query: 91  ALEKEQKKKNIERKRKEWEDTLASVTEEERSKLIESRKNLRKERMGKRTEEKEKKMERLN 150
              KE++K                            RK L + ++   ++  ++K  R +
Sbjct: 67  EKRKEKRK----------------------------RKKLERRQLESNSDGNDRKRVRRD 126

Query: 151 KARDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAMNGRCPSPGHLWLTGCNGEMETQL 210
            AR     +VID  F  LM   +I  L +QI  CYA N R   P   +LT   G+++  +
Sbjct: 127 VARS-SLRLVIDCSFDDLMVLKDIKKLHKQIQRCYAENRRASHPVQFYLTSHGGQLKKNM 186

Query: 211 -QKIPGFDKW---IIEKEHGSYIDALQDQKENLVYLTADAETVLDDLDLKKTYIIGGLVD 270
            +   G+  W    I+ EH S +     +KE+LVYLT+D+  VL DLD  K Y+IGGLVD
Sbjct: 187 DENDQGWVNWKDIHIKSEHYSEL----IKKEDLVYLTSDSPNVLKDLDESKAYVIGGLVD 246

Query: 271 RNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQ 330
            +  KG+T K+A   GI+ A+LP+  ++KM+S +VL VN V EI+L++LETRDW+++FF 
Sbjct: 247 HSHHKGLTFKQATSYGIEHAQLPLADFVKMNSRKVLAVNHVFEIILEFLETRDWQEAFFT 275

Query: 331 VVPQRK 333
           ++PQRK
Sbjct: 307 ILPQRK 275

BLAST of Sed0001140 vs. ExPASy Swiss-Prot
Match: O14214 (tRNA (guanine(9)-N1)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=trm10 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.7e-35
Identity = 103/301 (34.22%), Postives = 164/301 (54.49%), Query Frame = 0

Query: 65  EPPMSKNARKRLLKQQRFEAKKAEKKALEKEQKKKNIERKRKEWEDTLASVTEEERSKLI 124
           +P +SK+A KRL +QQ ++A + ++  + +E+K+                + +EER + I
Sbjct: 29  QPVLSKSALKRLKRQQEWDAGREKRAEMRREKKR----------------LRKEERKRKI 88

Query: 125 ESRKNLRKERMGKRTEEKEKKMERLNKARDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYC 184
           E+ + ++           +KK  RL K       IV+D  F  LM   EI+SL QQ+  C
Sbjct: 89  EAGEVVK----------SQKKRIRLGKVVPSSIRIVLDCAFDDLMNDKEINSLCQQVTRC 148

Query: 185 YAMNGRCPSPGHLWLTGCNGEMETQLQKI--PGFDKW-IIEKEHGSYIDALQDQKENLVY 244
           ++ N     P  L+ T   G ++T+   +     + W        SY++  + QKE LVY
Sbjct: 149 HSANRTALHPVELFATNFGGRLKTRQDFVLKGQQNNWKRYNPTTKSYLEEFESQKEKLVY 208

Query: 245 LTADAETVLDDLDLKKTYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQV 304
           L+AD++  + +LD  K YIIG +VD+NR+K +   KA EQGI+TAKLPI  Y+K++  ++
Sbjct: 209 LSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNKASEQGIKTAKLPIDEYIKITDRKI 268

Query: 305 LTVNQVIEILLKYLETRDWKDSFFQVVPQRK--RCEADSNSDHAVDGEENGYGDDEHETK 361
           LTVNQV EIL  +LE RDW+ +F +V+P+RK    ++D + D + D        D    K
Sbjct: 269 LTVNQVFEILSLWLEYRDWEKAFMEVIPKRKGILLKSDESFDVSEDTRSQSNQSDSELEK 303

BLAST of Sed0001140 vs. ExPASy Swiss-Prot
Match: Q3MHI8 (tRNA methyltransferase 10 homolog A OS=Bos taurus OX=9913 GN=TRMT10A PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 5.1e-34
Identity = 108/302 (35.76%), Postives = 166/302 (54.97%), Query Frame = 0

Query: 67  PMSKNARKRLLKQQRFEAKKAEKKALEKEQKKKNIERKRKEWEDTLASVTEEERSKLIES 126
           P+SK   K+L+KQ+++E    E++ L K+++K+  +RKRK+ E      +  + S     
Sbjct: 39  PISKRQMKKLMKQKQWE----EQRELRKQKRKE--KRKRKQLERQCQPESNSDGS----D 98

Query: 127 RKNLRKERMGKRTEEKEKKMERLNKARDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYA 186
           RK +R++ +                       ++ID  F  LM   +I  L +QI  CYA
Sbjct: 99  RKRIRRDVVHSPL------------------RLIIDCSFDSLMVLKDIKKLHKQIQRCYA 158

Query: 187 MNGRCPSPGHLWLTGCNGEMETQL-QKIPGFDKW---IIEKEHGSYIDALQDQKENLVYL 246
            N R   P   +LT   G+++  + +   G+  W    I+ EH S       QKE+L+YL
Sbjct: 159 ENRRALHPVQFYLTSHGGQLKKNMDENDKGWVNWKDIHIKPEHYSEF----IQKEDLIYL 218

Query: 247 TADAETVLDDLDLKKTYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVL 306
           T+D+  +L +LD  K Y+IGGLVD N  KG+T K+A + GI  A+LP+G+++KM+S +VL
Sbjct: 219 TSDSPNILKELDESKAYVIGGLVDHNHHKGLTYKQASDHGIDHAQLPLGNFVKMNSRKVL 278

Query: 307 TVNQVIEILLKYLETRDWKDSFFQVVPQRK-------RCEADSNSDHAVDGEENGYGDDE 358
            VN V EI+L+YLETRDW+++FF ++PQRK        CE+ S+ D      E G   D 
Sbjct: 279 AVNHVFEIILEYLETRDWQEAFFTILPQRKGAVPTDQACESCSH-DKKFARVEGGLNSDS 307

BLAST of Sed0001140 vs. ExPASy Swiss-Prot
Match: Q66JJ4 (tRNA methyltransferase 10 homolog A OS=Xenopus tropicalis OX=8364 GN=trmt10a PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.5e-33
Identity = 121/351 (34.47%), Postives = 179/351 (51.00%), Query Frame = 0

Query: 50  AKTEPPCSPSGCNPPEPPMSKNARKRLLKQQRFEAKKAEKK---ALEKEQKKKNIERKRK 109
           A TEP       N  +   S++A      Q+   A ++E K   A+ K Q KK +  K+K
Sbjct: 5   AVTEPGKCSIDPNTKDLLASQHAGNNTPLQENSSAPRSECKAQDAMSKRQMKKFL--KQK 64

Query: 110 EWEDTLASVTEEERSKLIESRKNLRKERMGKRTEEKEKKMER----------LNKARDCG 169
           +WED              E RK  RKE+  KR  E++ + E            ++ +   
Sbjct: 65  QWEDQR------------ELRKQKRKEKRQKRKLERQAQAEHNIDANSRKRFRHEVQPSA 124

Query: 170 QNIVIDLEFSHLMTPSEIHSLVQQIMYCYAMNGRCPSPGHLWLTGCNGEMETQLQKIPGF 229
             ++ID  F  LM   ++  L +QI  CYA N R   P  L+LT   G++++ + +   +
Sbjct: 125 LRLIIDCSFDDLMALRDVKKLNKQIRRCYAENRRAVHPVQLYLTSHGGQLKSNMDE---Y 184

Query: 230 DK-WI------IEKEHGSYIDALQDQKENLVYLTADAETVLDDLDLKKTYIIGGLVDRNR 289
           DK WI      I+ EH  Y D +  +KE+LVYLT+D+  VL +LD  K YIIGGLVD N 
Sbjct: 185 DKGWINWKDIHIKPEH--YKDLI--KKEDLVYLTSDSPEVLSELDETKAYIIGGLVDHNH 244

Query: 290 WKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVP 349
            KGIT KKA E GI  A+LP+G+++KM++ +VL VN V EI+L +LE ++WK++FF V+P
Sbjct: 245 HKGITYKKALELGISHAQLPLGNFVKMNTRKVLAVNHVFEIILAFLEKKEWKEAFFSVLP 304

Query: 350 QRK------------RCEADSNSDHAVDGEENGYGDDEHETKKTKCDEISS 369
           QRK             C A    D      ++   +        +C+E +S
Sbjct: 305 QRKGAIPLTETGEQPECRASEQEDGEDSDSDSSIDESATIQPSVRCEEQNS 334

BLAST of Sed0001140 vs. ExPASy TrEMBL
Match: A0A6J1I7W5 (tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111471663 PE=4 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 2.8e-160
Identity = 291/330 (88.18%), Postives = 315/330 (95.45%), Query Frame = 0

Query: 41  MEAVEDDHCAKTEPPCSPSGCNPPEPPMSKNARKRLLKQQRFEAKKAEKKALEKEQKKKN 100
           MEA+E+D  AK +PPCSPSGCNPPEPP+SKNARK+LLKQQR+EAKKAEKKA EKEQKKK+
Sbjct: 1   MEAIEEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 101 IERKRKEWEDTLASVTEEERSKLIESRKNLRKERMGKRTEEKEKKMERLNKARDCGQNIV 160
           IERKRKEWEDTLASVTEEER+KLIESR++LRKERMGKR+EEKEKK+ERLN+A+DCGQ I+
Sbjct: 61  IERKRKEWEDTLASVTEEERAKLIESRRSLRKERMGKRSEEKEKKIERLNRAKDCGQKII 120

Query: 161 IDLEFSHLMTPSEIHSLVQQIMYCYAMNGRCPSPGHLWLTGCNGEMETQLQKIPGFDKWI 220
           IDLEFS LMTPSEIHSLVQQIMYCYA+NGRCPSPGHLWLTGCNGEMETQLQ++PGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 221 IEKEHGSYIDALQDQKENLVYLTADAETVLDDLDLKKTYIIGGLVDRNRWKGITMKKAQE 280
           IEKEH SYIDALQDQKENLVYLTAD+ETVLDDLDLKK YIIGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKEHRSYIDALQDQKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQE 240

Query: 281 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCEADSNS 340
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWK SFFQVVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLKYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 341 DHAVDGEENGYGDDEHETKKTKCDEISSSN 371
           DHAVDGEENG  DDE E+KK KC E+SSSN
Sbjct: 301 DHAVDGEENGDRDDEQESKKIKCGEVSSSN 330

BLAST of Sed0001140 vs. ExPASy TrEMBL
Match: A0A6J1EPG7 (tRNA (guanine(9)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111434383 PE=4 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 1.4e-159
Identity = 291/330 (88.18%), Postives = 314/330 (95.15%), Query Frame = 0

Query: 41  MEAVEDDHCAKTEPPCSPSGCNPPEPPMSKNARKRLLKQQRFEAKKAEKKALEKEQKKKN 100
           MEA E+D  AK +PPCSPSGCNPPEPP+SKNARK+LLKQQR+EAKKAEKKA EKEQKKK+
Sbjct: 1   MEAREEDQSAKMDPPCSPSGCNPPEPPLSKNARKKLLKQQRYEAKKAEKKAQEKEQKKKD 60

Query: 101 IERKRKEWEDTLASVTEEERSKLIESRKNLRKERMGKRTEEKEKKMERLNKARDCGQNIV 160
           IERKRKEWE+TLASVTEEER+KLIESRK+LRKERMGKR+EEKEKK+ERLN+A+DCGQ IV
Sbjct: 61  IERKRKEWEETLASVTEEERAKLIESRKSLRKERMGKRSEEKEKKIERLNRAKDCGQKIV 120

Query: 161 IDLEFSHLMTPSEIHSLVQQIMYCYAMNGRCPSPGHLWLTGCNGEMETQLQKIPGFDKWI 220
           IDLEFS LMTPSEIHSLVQQIMYCYA+NGRCPSPGHLWLTGCNGEMETQLQ++PGFDKWI
Sbjct: 121 IDLEFSDLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 221 IEKEHGSYIDALQDQKENLVYLTADAETVLDDLDLKKTYIIGGLVDRNRWKGITMKKAQE 280
           IEKEH SYIDALQD KENLVYLTAD+ETVLDDLDLKK YIIGGLVDRNRWKGITM KAQE
Sbjct: 181 IEKEHRSYIDALQDHKENLVYLTADSETVLDDLDLKKIYIIGGLVDRNRWKGITMNKAQE 240

Query: 281 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCEADSNS 340
           QGIQTAKLPIGSYLKMSSSQVLTVNQV+EILL+Y+ETRDWK SFFQVVPQRKRCEA+ NS
Sbjct: 241 QGIQTAKLPIGSYLKMSSSQVLTVNQVLEILLRYIETRDWKASFFQVVPQRKRCEAECNS 300

Query: 341 DHAVDGEENGYGDDEHETKKTKCDEISSSN 371
           DHAVDG+ENG  DDE ETKKTKCDE+SSSN
Sbjct: 301 DHAVDGKENGDRDDEQETKKTKCDEVSSSN 330

BLAST of Sed0001140 vs. ExPASy TrEMBL
Match: A0A0A0LYJ3 (SAM-dependent MTase TRM10-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G173210 PE=4 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 2.8e-152
Identity = 281/330 (85.15%), Postives = 308/330 (93.33%), Query Frame = 0

Query: 41  MEAVEDDHCAKTEPPCSPSGCNPPEPPMSKNARKRLLKQQRFEAKKAEKKALEKEQKKKN 100
           MEAVE+ H  K +PPCS S CNPPEPP+SKNARK+LLKQQR+EAKKA+KK   KEQKKK+
Sbjct: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 101 IERKRKEWEDTLASVTEEERSKLIESRKNLRKERMGKRTEEKEKKMERLNKARDCGQNIV 160
           IERKRKEWE+ LASVTEEER+KLIESR+NLRKERMGKR+E++EKK+ERL+ ARD GQNIV
Sbjct: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120

Query: 161 IDLEFSHLMTPSEIHSLVQQIMYCYAMNGRCPSPGHLWLTGCNGEMETQLQKIPGFDKWI 220
           IDLEFSHLMTP EIHSLVQQIMYCYA+NGRCPSP HLWLTGCNGEMETQLQ++PGFDKWI
Sbjct: 121 IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 221 IEKEHGSYIDALQDQKENLVYLTADAETVLDDLDLKKTYIIGGLVDRNRWKGITMKKAQE 280
           IEKE  SYIDALQ+QKENLVYLTAD+ETVLDDLDLKK YI+GGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240

Query: 281 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCEADSNS 340
           Q IQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFF+VVPQRKRC  DSNS
Sbjct: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNS 300

Query: 341 DHAVDGEENGYGDDEHETKKTKCDEISSSN 371
           DH VDGEENG GD+++ETKKTKCDE+SSS+
Sbjct: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSD 330

BLAST of Sed0001140 vs. ExPASy TrEMBL
Match: A0A5D3CW30 (tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00280 PE=4 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 2.4e-151
Identity = 277/327 (84.71%), Postives = 303/327 (92.66%), Query Frame = 0

Query: 41  MEAVEDDHCAKTEPPCSPSGCNPPEPPMSKNARKRLLKQQRFEAKKAEKKALEKEQKKKN 100
           ME VE  H  K +PPCSPS CNPPEP +SKNARK+LLKQQR+EAKKA+KK   KEQKKK+
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 101 IERKRKEWEDTLASVTEEERSKLIESRKNLRKERMGKRTEEKEKKMERLNKARDCGQNIV 160
           IERKRKEWE+ LA VTEEER+KLIESR+NLRKERMGKR+E++EKK+ERLN A+DCGQNIV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 161 IDLEFSHLMTPSEIHSLVQQIMYCYAMNGRCPSPGHLWLTGCNGEMETQLQKIPGFDKWI 220
           IDLEFSHLMTPSEIHSLVQQIMYCYA+NGRCPSP HLWLTGCNGEM +QLQ++PGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 221 IEKEHGSYIDALQDQKENLVYLTADAETVLDDLDLKKTYIIGGLVDRNRWKGITMKKAQE 280
           IEKE  SYIDALQ+QKENLVYLTAD+ETVLDDLDLKK YI+GGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 281 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCEADSNS 340
           Q IQTA+LPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRC ADSNS
Sbjct: 241 QEIQTARLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCGADSNS 300

Query: 341 DHAVDGEENGYGDDEHETKKTKCDEIS 368
           DH VDGEENG GD++ ETKKTKCDE+S
Sbjct: 301 DHPVDGEENGDGDNKQETKKTKCDEVS 327

BLAST of Sed0001140 vs. ExPASy TrEMBL
Match: A0A1S3CL37 (tRNA (Guanine(9)-N1)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502202 PE=4 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 2.4e-151
Identity = 277/327 (84.71%), Postives = 303/327 (92.66%), Query Frame = 0

Query: 41  MEAVEDDHCAKTEPPCSPSGCNPPEPPMSKNARKRLLKQQRFEAKKAEKKALEKEQKKKN 100
           ME VE  H  K +PPCSPS CNPPEP +SKNARK+LLKQQR+EAKKA+KK   KEQKKK+
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 101 IERKRKEWEDTLASVTEEERSKLIESRKNLRKERMGKRTEEKEKKMERLNKARDCGQNIV 160
           IERKRKEWE+ LA VTEEER+KLIESR+NLRKERMGKR+E++EKK+ERLN A+DCGQNIV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 161 IDLEFSHLMTPSEIHSLVQQIMYCYAMNGRCPSPGHLWLTGCNGEMETQLQKIPGFDKWI 220
           IDLEFSHLMTPSEIHSLVQQIMYCYA+NGRCPSP HLWLTGCNGEM +QLQ++PGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 221 IEKEHGSYIDALQDQKENLVYLTADAETVLDDLDLKKTYIIGGLVDRNRWKGITMKKAQE 280
           IEKE  SYIDALQ+QKENLVYLTAD+ETVLDDLDLKK YI+GGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 281 QGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCEADSNS 340
           Q IQTA+LPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRC ADSNS
Sbjct: 241 QEIQTARLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCGADSNS 300

Query: 341 DHAVDGEENGYGDDEHETKKTKCDEIS 368
           DH VDGEENG GD++ ETKKTKCDE+S
Sbjct: 301 DHPVDGEENGDGDNKQETKKTKCDEVS 327

BLAST of Sed0001140 vs. TAIR 10
Match: AT5G47680.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: tRNA (guanine-N1-)-methyltransferase (InterPro:IPR016009), tRNA (guanine-N(1)-)-methyltransferase, metazoa (InterPro:IPR016653), tRNA (guanine-N1-)-methyltransferase, eukaryotic (InterPro:IPR007356); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 392.5 bits (1007), Expect = 3.8e-109
Identity = 214/320 (66.88%), Postives = 258/320 (80.62%), Query Frame = 0

Query: 46  DDHCAKTEPPCSPSGCNPPEPPMSKNARKRLLKQQRFEAKKAEKKALEKEQKKKNIERKR 105
           DD+    +P   P+  N   PP+SKNA+K+ LKQQR+EAKKAEKKA EKE K+K  ERK 
Sbjct: 8   DDNGCFDKPESEPAPVN-VSPPLSKNAQKKQLKQQRYEAKKAEKKAQEKEHKRKEGERKL 67

Query: 106 KEWEDTLASVTEEERSKLIESRKNLRKERMGKRTEEKEKKMERLNKARDCGQNIVIDLEF 165
           KEWE+TLA+ TEEER KLIESRK+LRKERM KR+EEKEKK+ERLN+A++ GQ IV+D++F
Sbjct: 68  KEWEETLANATEEERLKLIESRKSLRKERMEKRSEEKEKKIERLNQAKEIGQKIVVDVDF 127

Query: 166 SHLMTPSEIHSLVQQIMYCYAMNGRCPSPGHLWLTGCNGEMETQLQKIPGFDKWIIEKEH 225
           +HLM+ SEI SLVQQIMYCYA+NGR  SP HLWLTG  G+M T+L K+PGF+KW IEKE 
Sbjct: 128 AHLMSESEISSLVQQIMYCYAVNGRSTSPCHLWLTGVQGKMSTELDKLPGFEKWFIEKES 187

Query: 226 GSYIDALQDQKENLVYLTADAETVLDDLDLKKTYIIGGLVDRNRWKGITMKKAQEQGIQT 285
             YI+A+ DQK+NLVYLTAD+ETVLDDLD K  YIIGGLVDRNR+KGITM KAQEQGI+T
Sbjct: 188 RCYIEAMADQKDNLVYLTADSETVLDDLDPKHIYIIGGLVDRNRFKGITMTKAQEQGIKT 247

Query: 286 AKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCEADSNSDHAVD 345
           AKLPIG Y+KMSSSQVLTVNQV+EIL+K+LETRDWK +FF V+PQRKR   D      VD
Sbjct: 248 AKLPIGEYMKMSSSQVLTVNQVLEILVKFLETRDWKTAFFTVIPQRKRTGLD-----PVD 307

Query: 346 GEENGYGDDEHETKKTKCDE 366
             +  +  +EH+ K T+ +E
Sbjct: 308 CSKLEHISEEHQEKDTEANE 321