Sed0001098 (gene) Chayote v1

Overview
NameSed0001098
Typegene
OrganismSechium edule (Chayote v1)
DescriptionRING-type E3 ubiquitin transferase
LocationLG05: 40619774 .. 40623131 (+)
RNA-Seq ExpressionSed0001098
SyntenySed0001098
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAATGAAATTGGAGTGAACGCAATGGCAAATACGAATGATTCGAGTGTTTGTTCATTGATTCCCAATGGAAGCGAAGATATGGGTAAGAATTTGATCGAATTGAACGAACTAATCGAATCGATTGCGGGTTTTTCTGGGTTTCGAAAAACGCAATCGAAGGAATGTTTGAATTTGGTTAGGAGATTGAAGATGTTGGCGCCATTGTTGGAGGAGATTAGGGATCTTCACGATACGGTTCCTGCTCAGGCTCTGAGTCCCCATCTTGCTCATTTGAAGGAGGCTCTTGTTATGGCCAAAAGGTTACTGAAGAATTGCCACAATGGGAGTAAGATTTATCTGGTTAGTTTTTCTTTCAAATTTAAAGGGTTTTTTTGTGTTCTTTACTTTGGATTTTGATGGGGTTGTTCTTGATTGGTTTGATTTGTTTGGTTAGGCTTTTGAGAATGAGGCTGTCATGGCTAGGTTTCATGGTGTTTATGACAAATTGAAGGAGGCTCTTGATGAGCTGCCTTATGATAAGCTTGGAGTCTCAATTGAGTTGCAAGAACAAGTAGATTTCTTCACCTTATGAGACTTTGATTCTTTGTGTTATGAATCATTTCCATGATATGATGTTATCTTTAGGTTTCATGATGGATTTTATTTTCGGATTTTCCTTAAAGGCCTTGTTAATCGTTTGATCTTTCTTACTCTTCTCTTTTTAGTCATTTTGGTTGCACCTTAACATTGCTTTAGTCATTTTCTTAATATCGGTGGGTGTTCGGGCAATCTTGCGCGCACCAAACTATTGTTTAGGGACATAGTGTCTGACCCTACTACAGCTAGATGTCAAGGAAACACCTAGAAAATTAATTCATAGGTAGGTGACCTGCATCAATTAAACTCATAACATTTTTGTTTTTTTACTATACTTATGACCATTAGGCTACCCATCATCATTGCTTTAATCATTTGAAATGGGAAGATTCTTACTGTTTAGATTCCGTTTGAAAGCTGCTAGGTTTTTGAAAATTGTACTTAATTTCACACCATAGTTTTTAAAAACTTGGCTTAGATTTTGGAAACATTTCTTAAAAGATAATAAAACGAAGAAACTCATGGCTAGAAATAGTATTAATTAGTTTAATTTCCAAAAACTAAAACTAAATGTTATCGAATTGGTCTTAGTTTTTCTACTTTGCAAATGGAATTTTTAATCTTTATCTTGAACTTTTGATCTTTATATATGTATAGAAGTTATTCAATTTTCCTTGTAAATGGAACTTTTGATCTTTATCTTGAACTTTTGAAATAATGTCCAAGATATTAGTATTTTCTAAAATTTCTTTAGTTTATAATAAGTGTTGGAAGCTCATCGAGGAACTTGATGCACATTGTAGTTAACTATTATGATATTGGTTATTTTTCTCATTTTGTTGCTTACCAATTGATTACAATTGCTTCAATACTCCTTTTCTAAGTACTTGTATAATTATCAACTCAATTCATCCAGGTTGAGCTCATTTCTACGCAACTCAAAAGAGCGAAGTGTCGGAGAGATACACAAGACATGGAACTAGCGATGGACATGATTGTTGTGTTCTCTAAGAACGATGAGAGAAATGCTGATCCTGTTATACTCGAAAGATTGGCAAATAAATTAGAACTACGTAAAATTGCAGATTTGGAAGAAGAAACCATAGCTGTTCAAGAATTAATCAAACGTAGATGTCCACCAAATTCTGAAAGTGTCCAGCAAATTTTAGACCTTCTATCCAAGTTTAAACAAATTGCAGGTATGAACTCTAATGTTGTTCCTGATGGCCCCGTCGCATTGAAACGTCTGCAACGGTGTCGATCTACATTAATCCCTCATGAATTCCTCTGTCCAATTTCCTTAGAAATCATGTCAGATCCTGTCATTGTGGCTACTGGGCAGGTAACTTGAATAATCTTAACACTTTTTTACAACAGAATCTTTGCATTCTTAGCTTGATATTATATGAAGTGTTGTGATAGATGCTTTGGTTTTTGTTGTTCATTATTGAAGTATTGAAGTTGCACATGGTATCTTCATCTCAAAATTTGTAAGAGTTTGATTTGTTAGATCTTTGATCTTCATTGGAAAGATTAGTGACATACTCTGACATTTTGAATTTGAACTTCAGTAGGAATTGAAAGTTACTTGTATCTCGAGCTCGAGATAAAACCAGATTGCTTGACATTGAGATAACAATGTTATGATTTCAGTGTGTTACTTCTACAAAACTCTGCCAATAATTTGTAAACTGAGCCGGTTATCCTTGTCTCTTCGCAGACTTATGACCGAGAAAGTATTCAGCAATGGTTGGATGCTAATAACCGTACCTGCCCGAAAACCGGGCAAACTTTGATGCATTTGTCACTAGCTCCAAATTATGCCCTCAAGAACCTCATTGTGCAGTGGTCTCAAAAGTCTGACCAAGAATTGCCTAAAAAGAAAGTGCTTGCTGCATTGGGTGACACTCCACCTGAGCTCACTCAGGAAATCTCCTTAGTACTCAACAATCTATCATCAAGCCAGTTGGATGTCCAGAGAGAGGCTATCATCAAGATCCGGGTCCTTGCCAAGGAGAATCCCGAGAACCGAGTTTGGATTGCGAATAGCGGAGTCATCCCTCCATTGGTTAAGCTTCTCTCTTACCCGGATCTCAATTTCCAAGAACATACCGTGACCGCTCTTTTGAACTTGTCTATCGACGAAGCGAATAAAGTACTCATTGTAAAAGAAGGTGCCATTCCTGCTATCATAGAGATCCTGCAGCATGGAACAGAGGAGGCCAAGGAGAATTCTGCTGCTACCCTATTTAGCTTGTCAATGTTAGAAGAGAACAAGGTTCTGATCGGATCTCTAAACGGGATCCCGCCGTTAGTATCTCTTCTCCGAGACGGTACATTTCGAGGAAAGAGGGATGCTGCCACTGCACTCTTCAACCTGACATCAAACCAATCAAACAAGTCCCGAGCAATCAAAGCCGGTATCATACCGCCCCTTCGCTCTTTACTTAAGGATAAGAACTTGGGAATGATTGATGAAGCTTTATCAATCTTGTTAGTCCTTGCCTCGAACCCCGAGGGACGGGCCGAGATCAATAATAACTCGTTCATCGCAACTCTAGTCAACTTCATAAAAGTTGGCACACCGAAGAACAAGGAATGTGCTACGGCATTACTTCTAGAGCTCGGACTAAACAATTCGCCTTCCATTTTGGTTGCACTGCAGTTTGGTGTGTATGAACATTTGGTAGAGATAACAAAATCTGGGACAAGCAGAGCCCAGAGAAAAGCAAACTCACTTTTGCAGTACATGATCAAGTGTGAGCACATTTCTTGA

mRNA sequence

ATGGATAATGAAATTGGAGTGAACGCAATGGCAAATACGAATGATTCGAGTGTTTGTTCATTGATTCCCAATGGAAGCGAAGATATGGGTAAGAATTTGATCGAATTGAACGAACTAATCGAATCGATTGCGGGTTTTTCTGGGTTTCGAAAAACGCAATCGAAGGAATGTTTGAATTTGGTTAGGAGATTGAAGATGTTGGCGCCATTGTTGGAGGAGATTAGGGATCTTCACGATACGGTTCCTGCTCAGGCTCTGAGTCCCCATCTTGCTCATTTGAAGGAGGCTCTTGTTATGGCCAAAAGGTTACTGAAGAATTGCCACAATGGGAGTAAGATTTATCTGGCTTTTGAGAATGAGGCTGTCATGGCTAGGTTTCATGGTGTTTATGACAAATTGAAGGAGGCTCTTGATGAGCTGCCTTATGATAAGCTTGGAGTCTCAATTGAGTTGCAAGAACAAGTTGAGCTCATTTCTACGCAACTCAAAAGAGCGAAGTGTCGGAGAGATACACAAGACATGGAACTAGCGATGGACATGATTGTTGTGTTCTCTAAGAACGATGAGAGAAATGCTGATCCTGTTATACTCGAAAGATTGGCAAATAAATTAGAACTACGTAAAATTGCAGATTTGGAAGAAGAAACCATAGCTGTTCAAGAATTAATCAAACGTAGATGTCCACCAAATTCTGAAAGTGTCCAGCAAATTTTAGACCTTCTATCCAAGTTTAAACAAATTGCAGGTATGAACTCTAATGTTGTTCCTGATGGCCCCGTCGCATTGAAACGTCTGCAACGGTGTCGATCTACATTAATCCCTCATGAATTCCTCTGTCCAATTTCCTTAGAAATCATGTCAGATCCTGTCATTGTGGCTACTGGGCAGACTTATGACCGAGAAAGTATTCAGCAATGGTTGGATGCTAATAACCGTACCTGCCCGAAAACCGGGCAAACTTTGATGCATTTGTCACTAGCTCCAAATTATGCCCTCAAGAACCTCATTGTGCAGTGGTCTCAAAAGTCTGACCAAGAATTGCCTAAAAAGAAAGTGCTTGCTGCATTGGGTGACACTCCACCTGAGCTCACTCAGGAAATCTCCTTAGTACTCAACAATCTATCATCAAGCCAGTTGGATGTCCAGAGAGAGGCTATCATCAAGATCCGGGTCCTTGCCAAGGAGAATCCCGAGAACCGAGTTTGGATTGCGAATAGCGGAGTCATCCCTCCATTGGTTAAGCTTCTCTCTTACCCGGATCTCAATTTCCAAGAACATACCGTGACCGCTCTTTTGAACTTGTCTATCGACGAAGCGAATAAAGTACTCATTGTAAAAGAAGGTGCCATTCCTGCTATCATAGAGATCCTGCAGCATGGAACAGAGGAGGCCAAGGAGAATTCTGCTGCTACCCTATTTAGCTTGTCAATGTTAGAAGAGAACAAGGTTCTGATCGGATCTCTAAACGGGATCCCGCCGTTAGTATCTCTTCTCCGAGACGGTACATTTCGAGGAAAGAGGGATGCTGCCACTGCACTCTTCAACCTGACATCAAACCAATCAAACAAGTCCCGAGCAATCAAAGCCGGTATCATACCGCCCCTTCGCTCTTTACTTAAGGATAAGAACTTGGGAATGATTGATGAAGCTTTATCAATCTTGTTAGTCCTTGCCTCGAACCCCGAGGGACGGGCCGAGATCAATAATAACTCGTTCATCGCAACTCTAGTCAACTTCATAAAAGTTGGCACACCGAAGAACAAGGAATGTGCTACGGCATTACTTCTAGAGCTCGGACTAAACAATTCGCCTTCCATTTTGGTTGCACTGCAGTTTGGTGTGTATGAACATTTGGTAGAGATAACAAAATCTGGGACAAGCAGAGCCCAGAGAAAAGCAAACTCACTTTTGCAGTACATGATCAAGTGTGAGCACATTTCTTGA

Coding sequence (CDS)

ATGGATAATGAAATTGGAGTGAACGCAATGGCAAATACGAATGATTCGAGTGTTTGTTCATTGATTCCCAATGGAAGCGAAGATATGGGTAAGAATTTGATCGAATTGAACGAACTAATCGAATCGATTGCGGGTTTTTCTGGGTTTCGAAAAACGCAATCGAAGGAATGTTTGAATTTGGTTAGGAGATTGAAGATGTTGGCGCCATTGTTGGAGGAGATTAGGGATCTTCACGATACGGTTCCTGCTCAGGCTCTGAGTCCCCATCTTGCTCATTTGAAGGAGGCTCTTGTTATGGCCAAAAGGTTACTGAAGAATTGCCACAATGGGAGTAAGATTTATCTGGCTTTTGAGAATGAGGCTGTCATGGCTAGGTTTCATGGTGTTTATGACAAATTGAAGGAGGCTCTTGATGAGCTGCCTTATGATAAGCTTGGAGTCTCAATTGAGTTGCAAGAACAAGTTGAGCTCATTTCTACGCAACTCAAAAGAGCGAAGTGTCGGAGAGATACACAAGACATGGAACTAGCGATGGACATGATTGTTGTGTTCTCTAAGAACGATGAGAGAAATGCTGATCCTGTTATACTCGAAAGATTGGCAAATAAATTAGAACTACGTAAAATTGCAGATTTGGAAGAAGAAACCATAGCTGTTCAAGAATTAATCAAACGTAGATGTCCACCAAATTCTGAAAGTGTCCAGCAAATTTTAGACCTTCTATCCAAGTTTAAACAAATTGCAGGTATGAACTCTAATGTTGTTCCTGATGGCCCCGTCGCATTGAAACGTCTGCAACGGTGTCGATCTACATTAATCCCTCATGAATTCCTCTGTCCAATTTCCTTAGAAATCATGTCAGATCCTGTCATTGTGGCTACTGGGCAGACTTATGACCGAGAAAGTATTCAGCAATGGTTGGATGCTAATAACCGTACCTGCCCGAAAACCGGGCAAACTTTGATGCATTTGTCACTAGCTCCAAATTATGCCCTCAAGAACCTCATTGTGCAGTGGTCTCAAAAGTCTGACCAAGAATTGCCTAAAAAGAAAGTGCTTGCTGCATTGGGTGACACTCCACCTGAGCTCACTCAGGAAATCTCCTTAGTACTCAACAATCTATCATCAAGCCAGTTGGATGTCCAGAGAGAGGCTATCATCAAGATCCGGGTCCTTGCCAAGGAGAATCCCGAGAACCGAGTTTGGATTGCGAATAGCGGAGTCATCCCTCCATTGGTTAAGCTTCTCTCTTACCCGGATCTCAATTTCCAAGAACATACCGTGACCGCTCTTTTGAACTTGTCTATCGACGAAGCGAATAAAGTACTCATTGTAAAAGAAGGTGCCATTCCTGCTATCATAGAGATCCTGCAGCATGGAACAGAGGAGGCCAAGGAGAATTCTGCTGCTACCCTATTTAGCTTGTCAATGTTAGAAGAGAACAAGGTTCTGATCGGATCTCTAAACGGGATCCCGCCGTTAGTATCTCTTCTCCGAGACGGTACATTTCGAGGAAAGAGGGATGCTGCCACTGCACTCTTCAACCTGACATCAAACCAATCAAACAAGTCCCGAGCAATCAAAGCCGGTATCATACCGCCCCTTCGCTCTTTACTTAAGGATAAGAACTTGGGAATGATTGATGAAGCTTTATCAATCTTGTTAGTCCTTGCCTCGAACCCCGAGGGACGGGCCGAGATCAATAATAACTCGTTCATCGCAACTCTAGTCAACTTCATAAAAGTTGGCACACCGAAGAACAAGGAATGTGCTACGGCATTACTTCTAGAGCTCGGACTAAACAATTCGCCTTCCATTTTGGTTGCACTGCAGTTTGGTGTGTATGAACATTTGGTAGAGATAACAAAATCTGGGACAAGCAGAGCCCAGAGAAAAGCAAACTCACTTTTGCAGTACATGATCAAGTGTGAGCACATTTCTTGA

Protein sequence

MDNEIGVNAMANTNDSSVCSLIPNGSEDMGKNLIELNELIESIAGFSGFRKTQSKECLNLVRRLKMLAPLLEEIRDLHDTVPAQALSPHLAHLKEALVMAKRLLKNCHNGSKIYLAFENEAVMARFHGVYDKLKEALDELPYDKLGVSIELQEQVELISTQLKRAKCRRDTQDMELAMDMIVVFSKNDERNADPVILERLANKLELRKIADLEEETIAVQELIKRRCPPNSESVQQILDLLSKFKQIAGMNSNVVPDGPVALKRLQRCRSTLIPHEFLCPISLEIMSDPVIVATGQTYDRESIQQWLDANNRTCPKTGQTLMHLSLAPNYALKNLIVQWSQKSDQELPKKKVLAALGDTPPELTQEISLVLNNLSSSQLDVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTVTALLNLSIDEANKVLIVKEGAIPAIIEILQHGTEEAKENSAATLFSLSMLEENKVLIGSLNGIPPLVSLLRDGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMIDEALSILLVLASNPEGRAEINNNSFIATLVNFIKVGTPKNKECATALLLELGLNNSPSILVALQFGVYEHLVEITKSGTSRAQRKANSLLQYMIKCEHIS
Homology
BLAST of Sed0001098 vs. NCBI nr
Match: XP_038880222.1 (U-box domain-containing protein 15 [Benincasa hispida])

HSP 1 Score: 1017.3 bits (2629), Expect = 5.8e-293
Identity = 521/649 (80.28%), Postives = 591/649 (91.06%), Query Frame = 0

Query: 1   MDNEIGVNAMANTNDSSVCSLIPNGSEDMGKNLIELNELIESIAGFSGFRKTQSKECLNL 60
           M+   G N ++    SS+ + +P  S D+ K+L E +ELIE+I  FSGFRKTQSKECLNL
Sbjct: 1   MEKHYGSNGLSPC--SSISNGVPQESRDVTKDLKEAHELIEAIGAFSGFRKTQSKECLNL 60

Query: 61  VRRLKMLAPLLEEIRDLHDTVPAQALSPHLAHLKEALVMAKRLLKNCHNGSKIYLAFENE 120
           VRRLKML PLLEEIRDLHD VPA+AL  H+A+LKEALV+AKRLLKNCHNGSKIYLAFENE
Sbjct: 61  VRRLKMLVPLLEEIRDLHDMVPAEALISHIANLKEALVLAKRLLKNCHNGSKIYLAFENE 120

Query: 121 AVMARFHGVYDKLKEALDELPYDKLGVSIELQEQVELISTQLKRAKCRRDTQDMELAMDM 180
           AVMARFHGVYDKLKEALD +PYD+LGVS+EL+EQVEL+STQLKRAKCR+DTQDMELAMDM
Sbjct: 121 AVMARFHGVYDKLKEALDGIPYDELGVSVELKEQVELMSTQLKRAKCRKDTQDMELAMDM 180

Query: 181 IVVFSKNDERNADPVILERLANKLELRKIADLEEETIAVQELIKRRCPPNSESVQQILDL 240
           +VVFSKNDERNADPVILERLANKLELRKIADLE ETIAVQ+L++RR  PNSE++QQI++L
Sbjct: 181 MVVFSKNDERNADPVILERLANKLELRKIADLEAETIAVQKLVRRRGVPNSENLQQIIEL 240

Query: 241 LSKFKQIAGMNSNVVPDGPV---ALKRLQRCRSTLIPHEFLCPISLEIMSDPVIVATGQT 300
           L KFKQIAGM++ V PDGPV    L+RLQRC+ST+IPHEFLCPI+LE+M+DPVIVATGQT
Sbjct: 241 LGKFKQIAGMDNTVAPDGPVVSKCLQRLQRCQSTIIPHEFLCPITLEVMTDPVIVATGQT 300

Query: 301 YDRESIQQWLDANNRTCPKTGQTLMHLSLAPNYALKNLIVQWSQKSDQELPKKKVLAALG 360
           YDRESIQ+WL++N+RTCPKTGQTL+HLSLAPNYALKNLI+QW Q ++ +LPKK+V+A +G
Sbjct: 301 YDRESIQKWLNSNHRTCPKTGQTLVHLSLAPNYALKNLILQWCQNNNYDLPKKEVVAGMG 360

Query: 361 DTPPELTQEISLVLNNLSSSQLDVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKLLS 420
           DTPP+L +EIS +++NLSSSQLDVQREAIIKIRVL+KENPENRVWIANSGVIPPLVKLLS
Sbjct: 361 DTPPDLAREISSLVHNLSSSQLDVQREAIIKIRVLSKENPENRVWIANSGVIPPLVKLLS 420

Query: 421 YPDLNFQEHTVTALLNLSIDEANKVLIVKEGAIPAIIEILQHGTEEAKENSAATLFSLSM 480
           YPDLNFQEHTVTALLNLSID+ANK LI +EGAIPAIIEILQHGTEEAKENSAA LFSLSM
Sbjct: 421 YPDLNFQEHTVTALLNLSIDDANKRLIAREGAIPAIIEILQHGTEEAKENSAAALFSLSM 480

Query: 481 LEENKVLIGSLNGIPPLVSLLRDGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPLRS 540
           L+ENKVLIGSL GIPPLV LLRDGT RGK+DAATALFNL+ NQ+NKSRAIKAGIIPPL S
Sbjct: 481 LDENKVLIGSLKGIPPLVLLLRDGTIRGKKDAATALFNLSLNQTNKSRAIKAGIIPPLLS 540

Query: 541 LLKDKNLGMIDEALSILLVLASNPEGRAEINNNSFIATLVNFIKVGTPKNKECATALLLE 600
           LL+DKNLGM+DEALSILL+LAS+PEGR+EI NNSFI  LVN I  GTPKNKECAT+LLLE
Sbjct: 541 LLEDKNLGMVDEALSILLLLASHPEGRSEIGNNSFIEILVNIIINGTPKNKECATSLLLE 600

Query: 601 LGLNNSPSILVALQFGVYEHLVEITKSGTSRAQRKANSLLQYMIKCEHI 647
           LG N+SPSIL+ALQFGVYEHLVE+T+ GTSRAQRKA SLLQYM KCEHI
Sbjct: 601 LGRNHSPSILIALQFGVYEHLVELTRCGTSRAQRKATSLLQYMSKCEHI 647

BLAST of Sed0001098 vs. NCBI nr
Match: XP_004140059.2 (U-box domain-containing protein 15 [Cucumis sativus] >KAE8646827.1 hypothetical protein Csa_005549 [Cucumis sativus])

HSP 1 Score: 1008.8 bits (2607), Expect = 2.1e-290
Identity = 520/638 (81.50%), Postives = 580/638 (90.91%), Query Frame = 0

Query: 13  TNDSSVCSLIPNG----SEDMGKNLIELNELIESIAGFSGFRKTQSKECLNLVRRLKMLA 72
           +N  S CS I NG    + D+ K L E NELIE+I  FSGFRKTQSKECLNLVRRLKML 
Sbjct: 84  SNSLSPCSSISNGVLQETVDVTKCLTEANELIEAIGAFSGFRKTQSKECLNLVRRLKMLV 143

Query: 73  PLLEEIRDLHDTVPAQALSPHLAHLKEALVMAKRLLKNCHNGSKIYLAFENEAVMARFHG 132
           PLLEEIRDLHD +PA+ALS H++ LKEALV+AKRLLKNCHNGSKIYLAFENEAVMARFH 
Sbjct: 144 PLLEEIRDLHDMLPAEALSSHISLLKEALVLAKRLLKNCHNGSKIYLAFENEAVMARFHV 203

Query: 133 VYDKLKEALDELPYDKLGVSIELQEQVELISTQLKRAKCRRDTQDMELAMDMIVVFSKND 192
           VYDKLKEALD +PYD+LGVS+EL+EQVEL+STQLKRAKCR+DTQDMELAMDM+VVFSKND
Sbjct: 204 VYDKLKEALDGIPYDELGVSVELKEQVELMSTQLKRAKCRKDTQDMELAMDMMVVFSKND 263

Query: 193 ERNADPVILERLANKLELRKIADLEEETIAVQELIKRRCPPNSESVQQILDLLSKFKQIA 252
           ERNADPVILERLANKLELRKIADLE ETIAVQ+L++ R  PNSES+QQI+DLL KFKQIA
Sbjct: 264 ERNADPVILERLANKLELRKIADLEAETIAVQKLVRHRGVPNSESLQQIIDLLRKFKQIA 323

Query: 253 GMNSNVVPDGPVALKRLQRCRSTLIPHEFLCPISLEIMSDPVIVATGQTYDRESIQQWLD 312
           GM++NV PDGPV  K LQRC+STLIPHEFLCPI+LEIM+DPVIVATGQTYDRESIQ+WL+
Sbjct: 324 GMDNNVAPDGPVVSKSLQRCKSTLIPHEFLCPITLEIMTDPVIVATGQTYDRESIQKWLN 383

Query: 313 ANNRTCPKTGQTLMHLSLAPNYALKNLIVQWSQKSDQELPKKKVLAALGDTPPELTQEIS 372
           +N+RTCPK+GQTL+HLSLAPNYALKNLI+QW QK++ ELPKK+V+A +GDTP +L  EIS
Sbjct: 384 SNHRTCPKSGQTLVHLSLAPNYALKNLILQWCQKNNYELPKKEVVAGMGDTPSDLAGEIS 443

Query: 373 LVLNNLSSSQLDVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV 432
            +++NLSSSQLD+QREAIIKIRVL+KENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV
Sbjct: 444 SLVHNLSSSQLDIQREAIIKIRVLSKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV 503

Query: 433 TALLNLSIDEANKVLIVKEGAIPAIIEILQHGTEEAKENSAATLFSLSMLEENKVLIGSL 492
           TALLNLSID++NK LI +EGAIPAIIEILQ GTEEAKENSAA LFSLSML+ENKVLIGSL
Sbjct: 504 TALLNLSIDDSNKRLIAREGAIPAIIEILQRGTEEAKENSAAALFSLSMLDENKVLIGSL 563

Query: 493 NGIPPLVSLLRDGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMID 552
            GIPPLV LLRDGT RGK+DAATALFNL+ NQ+NKSRAIKAGII PL +LL+DKNLGM+D
Sbjct: 564 KGIPPLVLLLRDGTIRGKKDAATALFNLSLNQANKSRAIKAGIIQPLLALLEDKNLGMVD 623

Query: 553 EALSILLVLASNPEGRAEINNNSFIATLVNFIKVGTPKNKECATALLLELGLNNSPSILV 612
           EALSILL+LAS+PEGR+EI NNSFI  LVN I  GTPKNKECAT+LLLELG NNSPSILV
Sbjct: 624 EALSILLLLASHPEGRSEIGNNSFIEILVNIIIDGTPKNKECATSLLLELGRNNSPSILV 683

Query: 613 ALQFGVYEHLVEITKSGTSRAQRKANSLLQYMIKCEHI 647
           ALQFGVYEHLVE+T+ GTSRAQRKA SLLQYM KCEHI
Sbjct: 684 ALQFGVYEHLVELTRCGTSRAQRKATSLLQYMSKCEHI 721

BLAST of Sed0001098 vs. NCBI nr
Match: XP_008448190.1 (PREDICTED: U-box domain-containing protein 15 [Cucumis melo])

HSP 1 Score: 1007.7 bits (2604), Expect = 4.6e-290
Identity = 521/638 (81.66%), Postives = 579/638 (90.75%), Query Frame = 0

Query: 13  TNDSSVCSLIPNG----SEDMGKNLIELNELIESIAGFSGFRKTQSKECLNLVRRLKMLA 72
           +N  S CS I NG    + D+ K L E NELIE+I  FSGFRKTQSKECLNLVRRLKML 
Sbjct: 7   SNSLSPCSSISNGVLQETVDVTKCLAEANELIEAIGAFSGFRKTQSKECLNLVRRLKMLV 66

Query: 73  PLLEEIRDLHDTVPAQALSPHLAHLKEALVMAKRLLKNCHNGSKIYLAFENEAVMARFHG 132
           PLLEEIRDLHD +PA+ALS H++ LKEALV+AKRLLKNCHNGSKIYLAFENEAVMARFH 
Sbjct: 67  PLLEEIRDLHDMLPAEALSSHISLLKEALVLAKRLLKNCHNGSKIYLAFENEAVMARFHA 126

Query: 133 VYDKLKEALDELPYDKLGVSIELQEQVELISTQLKRAKCRRDTQDMELAMDMIVVFSKND 192
           VYDKLKEALD +PYD+LGVS+EL+EQVEL+STQLKRAKCR+DTQDMELAMDM+VVFSKND
Sbjct: 127 VYDKLKEALDGIPYDELGVSVELKEQVELMSTQLKRAKCRKDTQDMELAMDMMVVFSKND 186

Query: 193 ERNADPVILERLANKLELRKIADLEEETIAVQELIKRRCPPNSESVQQILDLLSKFKQIA 252
           ERNADPVILERLANKL+LRKIADLE ETIAVQ+LI+ R  PNSES+QQI+DLL KFKQIA
Sbjct: 187 ERNADPVILERLANKLDLRKIADLEAETIAVQKLIRHRGVPNSESLQQIIDLLCKFKQIA 246

Query: 253 GMNSNVVPDGPVALKRLQRCRSTLIPHEFLCPISLEIMSDPVIVATGQTYDRESIQQWLD 312
           GM++NV  DGPV  K L+RC+STLIPHEFLCPI+LEIM+DPVIVATGQTYDRESIQ+WL 
Sbjct: 247 GMDNNVALDGPVVSKSLRRCQSTLIPHEFLCPITLEIMTDPVIVATGQTYDRESIQKWLH 306

Query: 313 ANNRTCPKTGQTLMHLSLAPNYALKNLIVQWSQKSDQELPKKKVLAALGDTPPELTQEIS 372
           +N+RTCPKTGQTL+HLSLAPNYALKNLI+QW QK++  LPKK+V+A +GDTPP+L +EIS
Sbjct: 307 SNHRTCPKTGQTLVHLSLAPNYALKNLILQWCQKNNYNLPKKEVVAGMGDTPPDLAREIS 366

Query: 373 LVLNNLSSSQLDVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV 432
            +++NLSSSQLD+QREAIIKIRVL+KENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV
Sbjct: 367 SLVHNLSSSQLDIQREAIIKIRVLSKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV 426

Query: 433 TALLNLSIDEANKVLIVKEGAIPAIIEILQHGTEEAKENSAATLFSLSMLEENKVLIGSL 492
           TALLNLSID+ANK LI +EGAIPAIIEILQ GTEEAKENSAA LFSLSML+ENKVLIGSL
Sbjct: 427 TALLNLSIDDANKRLIAREGAIPAIIEILQCGTEEAKENSAAALFSLSMLDENKVLIGSL 486

Query: 493 NGIPPLVSLLRDGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMID 552
            GIPPLV LLRDGT RGK+DAATALFNL+ NQ+NKSRAIKAGII PL +LL+DKNLGM+D
Sbjct: 487 KGIPPLVLLLRDGTIRGKKDAATALFNLSLNQANKSRAIKAGIIQPLLALLEDKNLGMVD 546

Query: 553 EALSILLVLASNPEGRAEINNNSFIATLVNFIKVGTPKNKECATALLLELGLNNSPSILV 612
           EALSILL+LAS+PEGRAEI NNSFI  LVN I  GTPKNKECAT+LLLELG NNSPSILV
Sbjct: 547 EALSILLLLASHPEGRAEIGNNSFIEILVNIIIDGTPKNKECATSLLLELGRNNSPSILV 606

Query: 613 ALQFGVYEHLVEITKSGTSRAQRKANSLLQYMIKCEHI 647
           ALQFGVYEHLVE+T+ GTSRAQRKA SLLQYM KCEHI
Sbjct: 607 ALQFGVYEHLVELTRCGTSRAQRKATSLLQYMSKCEHI 644

BLAST of Sed0001098 vs. NCBI nr
Match: KAA0047841.1 (U-box domain-containing protein 15 [Cucumis melo var. makuwa])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.3e-289
Identity = 521/638 (81.66%), Postives = 579/638 (90.75%), Query Frame = 0

Query: 13  TNDSSVCSLIPNG----SEDMGKNLIELNELIESIAGFSGFRKTQSKECLNLVRRLKMLA 72
           +N  S CS I NG    + D+ K L E NELIE+I  FSGFRKTQSKECLNLVRRLKML 
Sbjct: 7   SNSLSPCSSISNGVLQETVDVTKCLAEANELIEAIGAFSGFRKTQSKECLNLVRRLKMLV 66

Query: 73  PLLEEIRDLHDTVPAQALSPHLAHLKEALVMAKRLLKNCHNGSKIYLAFENEAVMARFHG 132
           PLLEEIRDLHD +PA+ALS H++ LKEALV+AKRLLKNCHNGSKIYLAFENEAVMARFH 
Sbjct: 67  PLLEEIRDLHDMLPAEALSSHISLLKEALVLAKRLLKNCHNGSKIYLAFENEAVMARFHA 126

Query: 133 VYDKLKEALDELPYDKLGVSIELQEQVELISTQLKRAKCRRDTQDMELAMDMIVVFSKND 192
           VYDKLKEALD +PYD+LGVS+EL+EQVEL+STQLKRAKCR+DTQDMELAMDM+VVFSKND
Sbjct: 127 VYDKLKEALDGIPYDELGVSVELKEQVELMSTQLKRAKCRKDTQDMELAMDMMVVFSKND 186

Query: 193 ERNADPVILERLANKLELRKIADLEEETIAVQELIKRRCPPNSESVQQILDLLSKFKQIA 252
           ERNADPVILERLANKLELRKIADLE ETIAVQ+LI+ R  PNSES+QQI+DLL KFKQIA
Sbjct: 187 ERNADPVILERLANKLELRKIADLEAETIAVQKLIRHRGVPNSESLQQIIDLLCKFKQIA 246

Query: 253 GMNSNVVPDGPVALKRLQRCRSTLIPHEFLCPISLEIMSDPVIVATGQTYDRESIQQWLD 312
           GM++NV  DGPV  K L+RC+STLIP+EFLCPI+LEIM+DPVIVATGQTYDRESIQ+WL+
Sbjct: 247 GMDNNVALDGPVVSKSLRRCQSTLIPNEFLCPITLEIMTDPVIVATGQTYDRESIQKWLN 306

Query: 313 ANNRTCPKTGQTLMHLSLAPNYALKNLIVQWSQKSDQELPKKKVLAALGDTPPELTQEIS 372
           +N+RTCPKTGQTL+HLSLAPNYALKNLI+QW QK++  LPKK+V+A +GDTPP+L  EIS
Sbjct: 307 SNHRTCPKTGQTLVHLSLAPNYALKNLILQWCQKNNYNLPKKEVVAGMGDTPPDLAPEIS 366

Query: 373 LVLNNLSSSQLDVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV 432
            +++NLSSSQLD+QREAIIKIRVL+KENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV
Sbjct: 367 SLVHNLSSSQLDIQREAIIKIRVLSKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV 426

Query: 433 TALLNLSIDEANKVLIVKEGAIPAIIEILQHGTEEAKENSAATLFSLSMLEENKVLIGSL 492
           TALLNLSID+ANK LI +EGAIPAIIEILQ GTEEAKENSAA LFSLSML+ENKVLIGSL
Sbjct: 427 TALLNLSIDDANKRLIAREGAIPAIIEILQCGTEEAKENSAAALFSLSMLDENKVLIGSL 486

Query: 493 NGIPPLVSLLRDGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMID 552
            GIPPLV LLRDGT RGK+DAATALFNL+ NQ+NKSRAIKAGII PL +LL+DKNLGM+D
Sbjct: 487 KGIPPLVLLLRDGTIRGKKDAATALFNLSLNQANKSRAIKAGIIQPLLALLEDKNLGMVD 546

Query: 553 EALSILLVLASNPEGRAEINNNSFIATLVNFIKVGTPKNKECATALLLELGLNNSPSILV 612
           EALSILL+LAS+PEGRAEI NNSFI  LVN I  GTPKNKECAT+LLLELG NNSPSILV
Sbjct: 547 EALSILLLLASHPEGRAEIGNNSFIEILVNIIIDGTPKNKECATSLLLELGRNNSPSILV 606

Query: 613 ALQFGVYEHLVEITKSGTSRAQRKANSLLQYMIKCEHI 647
           ALQFGVYEHLVE+T+ GTSRAQRKA SLLQYM KCEHI
Sbjct: 607 ALQFGVYEHLVELTRCGTSRAQRKATSLLQYMSKCEHI 644

BLAST of Sed0001098 vs. NCBI nr
Match: XP_022140545.1 (U-box domain-containing protein 15 [Momordica charantia])

HSP 1 Score: 1003.8 bits (2594), Expect = 6.7e-289
Identity = 521/651 (80.03%), Postives = 582/651 (89.40%), Query Frame = 0

Query: 1   MDNEIGVNAMANTNDSSVCSLIPNGSEDMG----KNLIELNELIESIAGFSGFRKTQSKE 60
           M+ E GV      N  S CS   +G  D G    K L E NELI +I GFSGFRKTQSKE
Sbjct: 79  MEREFGV---IGNNGWSRCSPASSGGRDEGDEVIKELTEANELIAAIGGFSGFRKTQSKE 138

Query: 61  CLNLVRRLKMLAPLLEEIRDLHDTVPAQALSPHLAHLKEALVMAKRLLKNCHNGSKIYLA 120
           CLNLVRRLKML PLLEEIRDLH+ VPA+ALSPHLAHLKEALV+AKRLLKNCHNGSKIYLA
Sbjct: 139 CLNLVRRLKMLVPLLEEIRDLHNMVPAEALSPHLAHLKEALVLAKRLLKNCHNGSKIYLA 198

Query: 121 FENEAVMARFHGVYDKLKEALDELPYDKLGVSIELQEQVELISTQLKRAKCRRDTQDMEL 180
           FENEAVMARFH VYDKLKEALD +PYD+LGVS+ELQEQVEL+STQLKRAKCR+DTQDMEL
Sbjct: 199 FENEAVMARFHAVYDKLKEALDGMPYDELGVSVELQEQVELMSTQLKRAKCRKDTQDMEL 258

Query: 181 AMDMIVVFSKNDERNADPVILERLANKLELRKIADLEEETIAVQELIKR-RCPPNSESVQ 240
           AMDM+VVFSKNDERNADPVILERLANKLELRKIADLE ET AVQ+L+KR R  P+SES+Q
Sbjct: 259 AMDMMVVFSKNDERNADPVILERLANKLELRKIADLEAETEAVQKLVKRSRGMPSSESLQ 318

Query: 241 QILDLLSKFKQIAGMNSNVVPDGPVALKRLQRCRSTLIPHEFLCPISLEIMSDPVIVATG 300
           QIL+LL KFK IAGM++++ P GPV+ K L+RCRS +IPHEFLCPI+LEIM+DPVIVATG
Sbjct: 319 QILELLGKFKGIAGMDNDIAPHGPVSSKCLRRCRSAVIPHEFLCPITLEIMTDPVIVATG 378

Query: 301 QTYDRESIQQWLDANNRTCPKTGQTLMHLSLAPNYALKNLIVQWSQKSDQELPKKKVLAA 360
           QTYDRESIQ+WLD+N+RTCPKTGQTL HLS+APNYALKNLI+QW QK+  ELPKK     
Sbjct: 379 QTYDRESIQKWLDSNHRTCPKTGQTLAHLSVAPNYALKNLILQWCQKNHYELPKKD--GG 438

Query: 361 LGDTPPELTQEISLVLNNLSSSQLDVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKL 420
           LGDTPPEL +EIS +++NLSSSQLDV++EAII IRVL+KENPENRVWIANSGVIPPLVKL
Sbjct: 439 LGDTPPELAREISSLVHNLSSSQLDVRKEAIINIRVLSKENPENRVWIANSGVIPPLVKL 498

Query: 421 LSYPDLNFQEHTVTALLNLSIDEANKVLIVKEGAIPAIIEILQHGTEEAKENSAATLFSL 480
           LSYPDL FQEHTVTALLNLSID++NK LI +EGAIPAIIEILQHGTEEAKENSAA LFSL
Sbjct: 499 LSYPDLKFQEHTVTALLNLSIDDSNKRLIAREGAIPAIIEILQHGTEEAKENSAAALFSL 558

Query: 481 SMLEENKVLIGSLNGIPPLVSLLRDGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPL 540
           SML+ENKVLIG+LNGIPPLV LLRDGT RGK+DAATALFNL+ NQSNKSRAIK GIIPPL
Sbjct: 559 SMLDENKVLIGTLNGIPPLVDLLRDGTIRGKKDAATALFNLSLNQSNKSRAIKGGIIPPL 618

Query: 541 RSLLKDKNLGMIDEALSILLVLASNPEGRAEINNNSFIATLVNFIKVGTPKNKECATALL 600
             LL+DK+LGM+DEALSILL+L S+PEGRAE+++NSFI T+VN +K GTPKNKECAT+LL
Sbjct: 619 LRLLEDKSLGMVDEALSILLLLTSHPEGRAELSHNSFIETIVNIMKDGTPKNKECATSLL 678

Query: 601 LELGLNNSPSILVALQFGVYEHLVEITKSGTSRAQRKANSLLQYMIKCEHI 647
           LELGLNNSPSILVALQ+GVYEHL+E+T+SGTSRAQRKANSLLQYM KCEHI
Sbjct: 679 LELGLNNSPSILVALQYGVYEHLIELTRSGTSRAQRKANSLLQYMSKCEHI 724

BLAST of Sed0001098 vs. ExPASy Swiss-Prot
Match: Q681N2 (U-box domain-containing protein 15 OS=Arabidopsis thaliana OX=3702 GN=PUB15 PE=2 SV=2)

HSP 1 Score: 686.0 bits (1769), Expect = 4.0e-196
Identity = 360/612 (58.82%), Postives = 466/612 (76.14%), Query Frame = 0

Query: 35  ELNELIESIAGFSGFRKTQSKECLNLVRRLKMLAPLLEEIRDLHDTVPAQALSPHLAHLK 94
           ++  ++E +   +G+R+TQ KEC NLVRRLK+L P L+EIR       + +    L  L+
Sbjct: 56  DIVSIVEFLDQINGYRRTQQKECFNLVRRLKILIPFLDEIRGFE----SPSCKHFLNRLR 115

Query: 95  EALVMAKRLLKNCHNGSKIYLAFENEAVMARFHGVYDKLKEALDELPYDKLGVSIELQEQ 154
           +  + AK+LL+ C NGSKIY+A + E +M RFH +Y+KL   L + P+D+L +S + +++
Sbjct: 116 KVFLAAKKLLETCSNGSKIYMALDGETMMTRFHSIYEKLNRVLVKAPFDELMISGDAKDE 175

Query: 155 VELISTQLKRAKCRRDTQDMELAMDMIVVFSKNDERNADPVILERLANKLELRKIADLEE 214
           ++ +  QLK+AK R DTQD+ELA+DM+VVFSK D RNAD  I+ERLA KLEL+ I DL+ 
Sbjct: 176 IDSLCKQLKKAKRRTDTQDIELAVDMMVVFSKTDPRNADSAIIERLAKKLELQTIDDLKT 235

Query: 215 ETIAVQELIKRRCPPNSESVQQILDLLSKFKQIAGMNSNVVPDGPVALKRLQRCRSTLIP 274
           ETIA+Q LI+ +   N E+ Q I++LL+KFK++ G+ +  +   PV  K + +  S ++P
Sbjct: 236 ETIAIQSLIQDKGGLNIETKQHIIELLNKFKKLQGLEATDILYQPVINKAITKSTSLILP 295

Query: 275 HEFLCPISLEIMSDPVIVATGQTYDRESIQQWLDANNRTCPKTGQTLMHLSLAPNYALKN 334
           HEFLCPI+LEIM DPVI+ATGQTY++ESIQ+W DA ++TCPKT Q L HLSLAPN+ALKN
Sbjct: 296 HEFLCPITLEIMLDPVIIATGQTYEKESIQKWFDAGHKTCPKTRQELDHLSLAPNFALKN 355

Query: 335 LIVQWSQKSDQELPKKKVLAALGDTPPELTQEISLVLNNLSSSQLDVQREAIIKIRVLAK 394
           LI+QW +K++ ++P+K+V     D+  E   E+SL++  LSSSQL+ QR ++ ++R+LA+
Sbjct: 356 LIMQWCEKNNFKIPEKEVSP---DSQNEQKDEVSLLVEALSSSQLEEQRRSVKQMRLLAR 415

Query: 395 ENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTVTALLNLSIDEANKVLIVKEGAIPAII 454
           ENPENRV IAN+G IP LV+LLSYPD   QE+ VT LLNLSIDE NK LI  EGAIP II
Sbjct: 416 ENPENRVLIANAGAIPLLVQLLSYPDSGIQENAVTTLLNLSIDEVNKKLISNEGAIPNII 475

Query: 455 EILQHGTEEAKENSAATLFSLSMLEENKVLIGSLNGIPPLVSLLRDGTFRGKRDAATALF 514
           EIL++G  EA+ENSAA LFSLSML+ENKV IG  NGIPPLV LL+ GT RGK+DA TALF
Sbjct: 476 EILENGNREARENSAAALFSLSMLDENKVTIGLSNGIPPLVDLLQHGTLRGKKDALTALF 535

Query: 515 NLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMIDEALSILLVLASNPEGRAEINNNSFIA 574
           NL+ N +NK RAI AGI+ PL +LLKDKNLGMIDEALSILL+LAS+PEGR  I   SFI 
Sbjct: 536 NLSLNSANKGRAIDAGIVQPLLNLLKDKNLGMIDEALSILLLLASHPEGRQAIGQLSFIE 595

Query: 575 TLVNFIKVGTPKNKECATALLLELGLNNSPSILVALQFGVYEHLVEITKSGTSRAQRKAN 634
           TLV FI+ GTPKNKECAT++LLELG NNS  IL ALQFGVYE+LVEIT SGT+RAQRKAN
Sbjct: 596 TLVEFIRQGTPKNKECATSVLLELGSNNSSFILAALQFGVYEYLVEITTSGTNRAQRKAN 655

Query: 635 SLLQYMIKCEHI 647
           +L+Q + K E I
Sbjct: 656 ALIQLISKSEQI 660

BLAST of Sed0001098 vs. ExPASy Swiss-Prot
Match: Q9SNC6 (U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 5.4e-132
Identity = 272/619 (43.94%), Postives = 395/619 (63.81%), Query Frame = 0

Query: 36  LNELIESIAGFSGFRKTQSKECLNLVRRLKMLAPLLEEIRDLHDTVPAQALSPHLAHLKE 95
           L +++  IA  S +R T  K C NL RRLK+L P+ EEIR+ ++ +    L   L +LKE
Sbjct: 12  LIDVVNEIAAISDYRITVKKLCYNLARRLKLLVPMFEEIRESNEPISEDTLKT-LMNLKE 71

Query: 96  ALVMAKRLLKNCHNGSKIYLAFENEAVMARFHGVYDKLKEALDELPYDKLGVSIELQEQV 155
           A+  AK  LK C  GSKIYL  E E V ++   V  KL+++L ++PY++L +S E++EQV
Sbjct: 72  AMCSAKDYLKFCSQGSKIYLVMEREQVTSKLMEVSVKLEQSLSQIPYEELDISDEVREQV 131

Query: 156 ELISTQLKRAKCRRDTQDMELAMDMIVVFSKNDERNADPVILERLANKLELRKIADLEEE 215
           EL+ +Q +RAK R D  D EL  D+  + +K+ + +A   +LER+A KL L +I DL +E
Sbjct: 132 ELVLSQFRRAKGRVDVSDDELYEDLQSLCNKSSDVDAYQPVLERVAKKLHLMEIPDLAQE 191

Query: 216 TIAVQELIKRR---CPPNSESVQQILDLLSKF---------KQIAGMNSNVVPDGPVALK 275
           ++A+ E++         N E +  +L ++  F         +Q  G+NS    +G  +  
Sbjct: 192 SVALHEMVASSGGDVGENIEEMAMVLKMIKDFVQTEDDNGEEQKVGVNSR--SNGQTSTA 251

Query: 276 RLQRCRSTLIPHEFLCPISLEIMSDPVIVATGQTYDRESIQQWLDANNRTCPKTGQTLMH 335
             Q+    +IP +F CPISLE+M DPVIV++GQTY+R  I++W++  + TCPKT Q L  
Sbjct: 252 ASQKI--PVIPDDFRCPISLEMMRDPVIVSSGQTYERTCIEKWIEGGHSTCPKTQQALTS 311

Query: 336 LSLAPNYALKNLIVQWSQKSDQELPKK----KVLAALGDTPPELTQEISLVLNNLSSSQL 395
            +L PNY L++LI QW + +D E PK     +       + P    +I  ++  L+    
Sbjct: 312 TTLTPNYVLRSLIAQWCEANDIEPPKPPSSLRPRKVSSFSSPAEANKIEDLMWRLAYGNP 371

Query: 396 DVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTVTALLNLSIDEA 455
           + QR A  +IR+LAK N +NRV IA +G IP LV LLS PD   QEH+VTALLNLSI E 
Sbjct: 372 EDQRSAAGEIRLLAKRNADNRVAIAEAGAIPLLVGLLSTPDSRIQEHSVTALLNLSICEN 431

Query: 456 NKVLIVKEGAIPAIIEILQHGTEEAKENSAATLFSLSMLEENKVLIGSLNGIPPLVSLLR 515
           NK  IV  GAIP I+++L+ G+ EA+EN+AATLFSLS+++ENKV IG+L  IPPLV LL 
Sbjct: 432 NKGAIVSAGAIPGIVQVLKKGSMEARENAAATLFSLSVIDENKVTIGALGAIPPLVVLLN 491

Query: 516 DGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMIDEALSILLVLAS 575
           +GT RGK+DAATALFNL   Q NK +AI+AG+IP L  LL +   GM+DEAL+IL +L+S
Sbjct: 492 EGTQRGKKDAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEPGSGMVDEALAILAILSS 551

Query: 576 NPEGRAEINNNSFIATLVNFIKVGTPKNKECATALLLELGLNNSPSILVALQFGVYEHLV 635
           +PEG+A I ++  + +LV FI+ G+P+N+E A A+L+ L   +   ++ A + G+   L+
Sbjct: 552 HPEGKAIIGSSDAVPSLVEFIRTGSPRNRENAAAVLVHLCSGDPQHLVEAQKLGLMGPLI 611

Query: 636 EITKSGTSRAQRKANSLLQ 639
           ++  +GT R +RKA  LL+
Sbjct: 612 DLAGNGTDRGKRKAAQLLE 625

BLAST of Sed0001098 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 1.7e-125
Identity = 262/618 (42.39%), Postives = 396/618 (64.08%), Query Frame = 0

Query: 36  LNELIESIAGFSGFRKTQSKECLNLVRRLKMLAPLLEEIRDLHDTVPAQALSPHLAHLKE 95
           L + ++ I+GFS  R    K   +LVRR+ +L+P  EE+ D++  +    ++   A ++ 
Sbjct: 16  LVDSVKEISGFSSSRGFIGKIQGDLVRRITLLSPFFEELIDVNVELKKDQITGFEA-MRI 75

Query: 96  ALVMAKRLLKNCHNGSKIYLAFENEAVMARFHGVYDKLKEALDELPYDKLGVSIELQEQV 155
           AL  +  L ++ + GSK++  F+ ++++ +F  +  +++ AL ++PY+K+ VS E++EQV
Sbjct: 76  ALDSSLELFRSVNGGSKLFQLFDRDSLVEKFRDMTVEIEAALSQIPYEKIEVSEEVREQV 135

Query: 156 ELISTQLKRAKCRRDTQDMELAMDMIVVFSKNDERNADPVILERLANKLELRKIADLEEE 215
           +L+  Q KRAK R +  D++L+ D+ +  +  D    DP+IL+RL+ +L+L  I +L++E
Sbjct: 136 QLLHFQFKRAKERWEESDLQLSHDLAMAENVMD---PDPIILKRLSQELQLTTIDELKKE 195

Query: 216 TIAVQELIKRRCPPNSESVQQILDLLSKFKQIAGMNSNVVPDGPVALKRLQRCRSTLIPH 275
           + A+ E          +  +++  LL        M S+  PD     + + R RS +IP 
Sbjct: 196 SHAIHEYFLSYDGDPDDCFERMSSLLKNLVDFVTMESS-DPDPSTGSRIVSRHRSPVIPE 255

Query: 276 EFLCPISLEIMSDPVIVATGQTYDRESIQQWLDANNRTCPKTGQTLMHLSLAPNYALKNL 335
            F CPISLE+M DPVIV+TGQTY+R SIQ+WLDA ++TCPK+ +TL+H  L PNY LK+L
Sbjct: 256 YFRCPISLELMKDPVIVSTGQTYERSSIQKWLDAGHKTCPKSQETLLHAGLTPNYVLKSL 315

Query: 336 IVQWSQKSDQELPKKK------VLAALGDTPPELTQEISLVLNNLSSSQLDVQREAIIKI 395
           I  W + +  ELP+ +       +     +  + T  +SL L  L++   + QR A  ++
Sbjct: 316 IALWCESNGIELPQNQGSCRTTKIGGSSSSDCDRTFVLSL-LEKLANGTTEQQRAAAGEL 375

Query: 396 RVLAKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTVTALLNLSIDEANKVLIVKEGA 455
           R+LAK N +NRV IA +G IP LV+LLS PD   QEH+VTALLNLSI+E NK  IV  GA
Sbjct: 376 RLLAKRNVDNRVCIAEAGAIPLLVELLSSPDPRTQEHSVTALLNLSINEGNKGAIVDAGA 435

Query: 456 IPAIIEILQHGTEEAKENSAATLFSLSMLEENKVLIGSLNGIPPLVSLLRDGTFRGKRDA 515
           I  I+E+L++G+ EA+EN+AATLFSLS+++ENKV IG+   I  L+SLL +GT RGK+DA
Sbjct: 436 ITDIVEVLKNGSMEARENAAATLFSLSVIDENKVAIGAAGAIQALISLLEEGTRRGKKDA 495

Query: 516 ATALFNLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMIDEALSILLVLASNPEGRAEINN 575
           ATA+FNL   Q NKSRA+K GI+ PL  LLKD   GM+DEAL+IL +L++N EG+  I  
Sbjct: 496 ATAIFNLCIYQGNKSRAVKGGIVDPLTRLLKDAGGGMVDEALAILAILSTNQEGKTAIAE 555

Query: 576 NSFIATLVNFIKVGTPKNKECATALLLELGLNNSPSILVALQFGVYEHLVEITKSGTSRA 635
              I  LV  I+ G+P+N+E A A+L  L + N   + VA + G    L E+T++GT RA
Sbjct: 556 AESIPVLVEIIRTGSPRNRENAAAILWYLCIGNIERLNVAREVGADVALKELTENGTDRA 615

Query: 636 QRKANSLLQYMIKCEHIS 648
           +RKA SLL+ + + E ++
Sbjct: 616 KRKAASLLELIQQTEGVA 627

BLAST of Sed0001098 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 7.0e-124
Identity = 269/604 (44.54%), Postives = 371/604 (61.42%), Query Frame = 0

Query: 41  ESIAGFSGFRKTQSKECLNLVRRLKMLAPLLEEIRDLHDTVPAQALSPHLAHLKEALVMA 100
           + IA     R    + C +L RR+++LAPLL       D +PA + S     L +AL  A
Sbjct: 7   DEIAALPEPRGPLRRPCADLSRRVRLLAPLL-------DHLPASSSSSSSTPLADALGAA 66

Query: 101 KRLLKNCHNGSKIYLAFENEAVMARFHGVYDKLKEALDELPYDKLGVSIELQEQVELIST 160
           + LL+   +GSKI  A   +A +  F GV  ++  ALD LPY+   +  E+QEQV L+ +
Sbjct: 67  RDLLRKTRDGSKIDQAMRGDAFLDEFAGVNRQIHLALDALPYNTFHMPQEVQEQVALVHS 126

Query: 161 QLKRAKCRRDTQDMELAMDMIVVFSKNDERNADPVILERLANKLELRKIADLEEETIAVQ 220
           Q +RA  R D  D +L+MD+    + N    +DP +L R+++KL+L  +AD++ E+IA+ 
Sbjct: 127 QFQRASTRTDPPDTQLSMDLAWALTDNP---SDPALLTRISHKLQLHTMADMKNESIALH 186

Query: 221 ELIKRRCPPNSESVQQILDLLSKFKQIAGMNSNVVPDGPVALKRLQRCRSTLIPHEFLCP 280
            ++          V Q+  LL K K    +  +   D         + RS +IP EF CP
Sbjct: 187 NMVISTAGEPDGCVDQMSSLLKKLKDCV-VTEDHANDALTTRSASIKHRSPIIPDEFRCP 246

Query: 281 ISLEIMSDPVIVATGQTYDRESIQQWLDANNRTCPKTGQTLMHLSLAPNYALKNLIVQWS 340
           ISLE+M DPVIV++GQTY+R  IQ+WLD+ ++TCPKT Q L H SL PN+ LK+LI QW 
Sbjct: 247 ISLELMQDPVIVSSGQTYERSCIQKWLDSGHKTCPKTQQPLSHTSLTPNFVLKSLISQWC 306

Query: 341 QKSDQELPKKKVLA----ALGDTPPELTQEISLVLNNLSSSQLDVQREAIIKIRVLAKEN 400
           + +  ELPK K  +    A   +  +    +SL +N L S   D QR A  +IR+LAK N
Sbjct: 307 EANGIELPKNKQNSRDKKAAKSSDYDHAGLVSL-MNRLRSGNQDEQRAAAGEIRLLAKRN 366

Query: 401 PENRVWIANSGVIPPLVKLLSYPDLNFQEHTVTALLNLSIDEANKVLIVKEGAIPAIIEI 460
             NR+ IA +G IP LV LLS  D   QEH VTALLNLSI E NK  IV   AIP I+E+
Sbjct: 367 VNNRICIAEAGAIPLLVNLLSSSDPRTQEHAVTALLNLSIHENNKASIVDSHAIPKIVEV 426

Query: 461 LQHGTEEAKENSAATLFSLSMLEENKVLIGSLNGIPPLVSLLRDGTFRGKRDAATALFNL 520
           L+ G+ E +EN+AATLFSLS+++ENKV IG+   IPPL++LL DG+ RGK+DAATA+FNL
Sbjct: 427 LKTGSMETRENAAATLFSLSVVDENKVTIGAAGAIPPLINLLCDGSPRGKKDAATAIFNL 486

Query: 521 TSNQSNKSRAIKAGIIPPLRSLLKDKNLGMIDEALSILLVLASNPEGRAEINNNSFIATL 580
              Q NK RA+KAGI+  L + L D   GMIDEALS+L +LA NPEG+  I  +  I  L
Sbjct: 487 CIYQGNKVRAVKAGIVIHLMNFLVDPTGGMIDEALSLLSILAGNPEGKIVIARSEPIPPL 546

Query: 581 VNFIKVGTPKNKECATALLLELGLNNSPSILVALQFGVYEHLVEITKSGTSRAQRKANSL 640
           V  IK G+P+N+E A A+L  L   ++   L A   GV + L E++++GT RA+RKA+S+
Sbjct: 547 VEVIKTGSPRNRENAAAILWLLCSADTEQTLAAKAAGVEDALKELSETGTDRAKRKASSI 598

BLAST of Sed0001098 vs. ExPASy Swiss-Prot
Match: Q9ZV31 (U-box domain-containing protein 12 OS=Arabidopsis thaliana OX=3702 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 4.0e-119
Identity = 260/621 (41.87%), Postives = 390/621 (62.80%), Query Frame = 0

Query: 36  LNELIESIAGFSGFRKTQSKECLNLVRRLKMLAPLLEEIRDLHDTVPAQALSPHLAHLKE 95
           L + I  IA  S       K C NL RRL +L P+LEEIRD  ++  +  +   L  +K+
Sbjct: 13  LIDSINEIASISDSVTPMKKHCANLSRRLSLLLPMLEEIRDNQES--SSEVVNALLSVKQ 72

Query: 96  ALVMAKRLLKNCHNGSKIYLAFENEAVMARFHGVYDKLKEALDELPYDKLGVSIELQEQV 155
           +L+ AK LL    + SKIYL  E + VM +F  V   L++AL  +PY+ L +S EL+EQV
Sbjct: 73  SLLHAKDLLSFVSHVSKIYLVLERDQVMVKFQKVTSLLEQALSIIPYENLEISDELKEQV 132

Query: 156 ELISTQLKRAKCRR--DTQDMELAMDMIVVFS-KNDERNADPVILERLANKLELRKIADL 215
           EL+  QL+R+  +R  D  D EL  D++ ++S +     +D  ++ R+A KL+L  I DL
Sbjct: 133 ELVLVQLRRSLGKRGGDVYDDELYKDVLSLYSGRGSVMESD--MVRRVAEKLQLMTITDL 192

Query: 216 EEETIAVQELIKRRCPPN-SESVQQILDLLSKFKQIAGMNSNVVPDGPVALK-RLQRCRS 275
            +E++A+ +++      +  ES +++  +L K K      +  + D P+ LK  L + R 
Sbjct: 193 TQESLALLDMVSSSGGDDPGESFEKMSMVLKKIKDFVQTYNPNLDDAPLRLKSSLPKSRD 252

Query: 276 -----TLIPHEFLCPISLEIMSDPVIVATGQTYDRESIQQWLDANNRTCPKTGQTLMHLS 335
                 + P EF CPISLE+M+DPVIV++GQTY+RE I++WL+  + TCPKT +TL    
Sbjct: 253 DDRDMLIPPEEFRCPISLELMTDPVIVSSGQTYERECIKKWLEGGHLTCPKTQETLTSDI 312

Query: 336 LAPNYALKNLIVQWSQKSDQELPKKKVL-------AALGDTPPELTQEISLVLNNLSSSQ 395
           + PNY L++LI QW + +  E PK+  +       ++    P +   +I  +L  L+S Q
Sbjct: 313 MTPNYVLRSLIAQWCESNGIEPPKRPNISQPSSKASSSSSAPDDEHNKIEELLLKLTSQQ 372

Query: 396 LDVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKLLSYP-DLNFQEHTVTALLNLSID 455
            + +R A  +IR+LAK+N  NRV IA SG IP LV LL+   D   QEH VT++LNLSI 
Sbjct: 373 PEDRRSAAGEIRLLAKQNNHNRVAIAASGAIPLLVNLLTISNDSRTQEHAVTSILNLSIC 432

Query: 456 EANKVLIV-KEGAIPAIIEILQHGTEEAKENSAATLFSLSMLEENKVLIGSLNGIPPLVS 515
           + NK  IV   GA+P I+ +LQ G+ EA+EN+AATLFSLS+++ENKV IG+   IPPLV+
Sbjct: 433 QENKGKIVYSSGAVPGIVHVLQKGSMEARENAAATLFSLSVIDENKVTIGAAGAIPPLVT 492

Query: 516 LLRDGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMIDEALSILLV 575
           LL +G+ RGK+DAATALFNL   Q NK +A++AG++P L  LL +   GM+DE+LSIL +
Sbjct: 493 LLSEGSQRGKKDAATALFNLCIFQGNKGKAVRAGLVPVLMRLLTEPESGMVDESLSILAI 552

Query: 576 LASNPEGRAEINNNSFIATLVNFIKVGTPKNKECATALLLELGLNNSPSILVALQFGVYE 635
           L+S+P+G++E+     +  LV+FI+ G+P+NKE + A+L+ L   N   ++ A + G+ +
Sbjct: 553 LSSHPDGKSEVGAADAVPVLVDFIRSGSPRNKENSAAVLVHLCSWNQQHLIEAQKLGIMD 612

Query: 636 HLVEITKSGTSRAQRKANSLL 638
            L+E+ ++GT R +RKA  LL
Sbjct: 613 LLIEMAENGTDRGKRKAAQLL 629

BLAST of Sed0001098 vs. ExPASy TrEMBL
Match: A0A0A0KE27 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G118880 PE=4 SV=1)

HSP 1 Score: 1008.8 bits (2607), Expect = 1.0e-290
Identity = 520/638 (81.50%), Postives = 580/638 (90.91%), Query Frame = 0

Query: 13  TNDSSVCSLIPNG----SEDMGKNLIELNELIESIAGFSGFRKTQSKECLNLVRRLKMLA 72
           +N  S CS I NG    + D+ K L E NELIE+I  FSGFRKTQSKECLNLVRRLKML 
Sbjct: 7   SNSLSPCSSISNGVLQETVDVTKCLTEANELIEAIGAFSGFRKTQSKECLNLVRRLKMLV 66

Query: 73  PLLEEIRDLHDTVPAQALSPHLAHLKEALVMAKRLLKNCHNGSKIYLAFENEAVMARFHG 132
           PLLEEIRDLHD +PA+ALS H++ LKEALV+AKRLLKNCHNGSKIYLAFENEAVMARFH 
Sbjct: 67  PLLEEIRDLHDMLPAEALSSHISLLKEALVLAKRLLKNCHNGSKIYLAFENEAVMARFHV 126

Query: 133 VYDKLKEALDELPYDKLGVSIELQEQVELISTQLKRAKCRRDTQDMELAMDMIVVFSKND 192
           VYDKLKEALD +PYD+LGVS+EL+EQVEL+STQLKRAKCR+DTQDMELAMDM+VVFSKND
Sbjct: 127 VYDKLKEALDGIPYDELGVSVELKEQVELMSTQLKRAKCRKDTQDMELAMDMMVVFSKND 186

Query: 193 ERNADPVILERLANKLELRKIADLEEETIAVQELIKRRCPPNSESVQQILDLLSKFKQIA 252
           ERNADPVILERLANKLELRKIADLE ETIAVQ+L++ R  PNSES+QQI+DLL KFKQIA
Sbjct: 187 ERNADPVILERLANKLELRKIADLEAETIAVQKLVRHRGVPNSESLQQIIDLLRKFKQIA 246

Query: 253 GMNSNVVPDGPVALKRLQRCRSTLIPHEFLCPISLEIMSDPVIVATGQTYDRESIQQWLD 312
           GM++NV PDGPV  K LQRC+STLIPHEFLCPI+LEIM+DPVIVATGQTYDRESIQ+WL+
Sbjct: 247 GMDNNVAPDGPVVSKSLQRCKSTLIPHEFLCPITLEIMTDPVIVATGQTYDRESIQKWLN 306

Query: 313 ANNRTCPKTGQTLMHLSLAPNYALKNLIVQWSQKSDQELPKKKVLAALGDTPPELTQEIS 372
           +N+RTCPK+GQTL+HLSLAPNYALKNLI+QW QK++ ELPKK+V+A +GDTP +L  EIS
Sbjct: 307 SNHRTCPKSGQTLVHLSLAPNYALKNLILQWCQKNNYELPKKEVVAGMGDTPSDLAGEIS 366

Query: 373 LVLNNLSSSQLDVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV 432
            +++NLSSSQLD+QREAIIKIRVL+KENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV
Sbjct: 367 SLVHNLSSSQLDIQREAIIKIRVLSKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV 426

Query: 433 TALLNLSIDEANKVLIVKEGAIPAIIEILQHGTEEAKENSAATLFSLSMLEENKVLIGSL 492
           TALLNLSID++NK LI +EGAIPAIIEILQ GTEEAKENSAA LFSLSML+ENKVLIGSL
Sbjct: 427 TALLNLSIDDSNKRLIAREGAIPAIIEILQRGTEEAKENSAAALFSLSMLDENKVLIGSL 486

Query: 493 NGIPPLVSLLRDGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMID 552
            GIPPLV LLRDGT RGK+DAATALFNL+ NQ+NKSRAIKAGII PL +LL+DKNLGM+D
Sbjct: 487 KGIPPLVLLLRDGTIRGKKDAATALFNLSLNQANKSRAIKAGIIQPLLALLEDKNLGMVD 546

Query: 553 EALSILLVLASNPEGRAEINNNSFIATLVNFIKVGTPKNKECATALLLELGLNNSPSILV 612
           EALSILL+LAS+PEGR+EI NNSFI  LVN I  GTPKNKECAT+LLLELG NNSPSILV
Sbjct: 547 EALSILLLLASHPEGRSEIGNNSFIEILVNIIIDGTPKNKECATSLLLELGRNNSPSILV 606

Query: 613 ALQFGVYEHLVEITKSGTSRAQRKANSLLQYMIKCEHI 647
           ALQFGVYEHLVE+T+ GTSRAQRKA SLLQYM KCEHI
Sbjct: 607 ALQFGVYEHLVELTRCGTSRAQRKATSLLQYMSKCEHI 644

BLAST of Sed0001098 vs. ExPASy TrEMBL
Match: A0A1S3BIJ2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103490456 PE=4 SV=1)

HSP 1 Score: 1007.7 bits (2604), Expect = 2.2e-290
Identity = 521/638 (81.66%), Postives = 579/638 (90.75%), Query Frame = 0

Query: 13  TNDSSVCSLIPNG----SEDMGKNLIELNELIESIAGFSGFRKTQSKECLNLVRRLKMLA 72
           +N  S CS I NG    + D+ K L E NELIE+I  FSGFRKTQSKECLNLVRRLKML 
Sbjct: 7   SNSLSPCSSISNGVLQETVDVTKCLAEANELIEAIGAFSGFRKTQSKECLNLVRRLKMLV 66

Query: 73  PLLEEIRDLHDTVPAQALSPHLAHLKEALVMAKRLLKNCHNGSKIYLAFENEAVMARFHG 132
           PLLEEIRDLHD +PA+ALS H++ LKEALV+AKRLLKNCHNGSKIYLAFENEAVMARFH 
Sbjct: 67  PLLEEIRDLHDMLPAEALSSHISLLKEALVLAKRLLKNCHNGSKIYLAFENEAVMARFHA 126

Query: 133 VYDKLKEALDELPYDKLGVSIELQEQVELISTQLKRAKCRRDTQDMELAMDMIVVFSKND 192
           VYDKLKEALD +PYD+LGVS+EL+EQVEL+STQLKRAKCR+DTQDMELAMDM+VVFSKND
Sbjct: 127 VYDKLKEALDGIPYDELGVSVELKEQVELMSTQLKRAKCRKDTQDMELAMDMMVVFSKND 186

Query: 193 ERNADPVILERLANKLELRKIADLEEETIAVQELIKRRCPPNSESVQQILDLLSKFKQIA 252
           ERNADPVILERLANKL+LRKIADLE ETIAVQ+LI+ R  PNSES+QQI+DLL KFKQIA
Sbjct: 187 ERNADPVILERLANKLDLRKIADLEAETIAVQKLIRHRGVPNSESLQQIIDLLCKFKQIA 246

Query: 253 GMNSNVVPDGPVALKRLQRCRSTLIPHEFLCPISLEIMSDPVIVATGQTYDRESIQQWLD 312
           GM++NV  DGPV  K L+RC+STLIPHEFLCPI+LEIM+DPVIVATGQTYDRESIQ+WL 
Sbjct: 247 GMDNNVALDGPVVSKSLRRCQSTLIPHEFLCPITLEIMTDPVIVATGQTYDRESIQKWLH 306

Query: 313 ANNRTCPKTGQTLMHLSLAPNYALKNLIVQWSQKSDQELPKKKVLAALGDTPPELTQEIS 372
           +N+RTCPKTGQTL+HLSLAPNYALKNLI+QW QK++  LPKK+V+A +GDTPP+L +EIS
Sbjct: 307 SNHRTCPKTGQTLVHLSLAPNYALKNLILQWCQKNNYNLPKKEVVAGMGDTPPDLAREIS 366

Query: 373 LVLNNLSSSQLDVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV 432
            +++NLSSSQLD+QREAIIKIRVL+KENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV
Sbjct: 367 SLVHNLSSSQLDIQREAIIKIRVLSKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV 426

Query: 433 TALLNLSIDEANKVLIVKEGAIPAIIEILQHGTEEAKENSAATLFSLSMLEENKVLIGSL 492
           TALLNLSID+ANK LI +EGAIPAIIEILQ GTEEAKENSAA LFSLSML+ENKVLIGSL
Sbjct: 427 TALLNLSIDDANKRLIAREGAIPAIIEILQCGTEEAKENSAAALFSLSMLDENKVLIGSL 486

Query: 493 NGIPPLVSLLRDGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMID 552
            GIPPLV LLRDGT RGK+DAATALFNL+ NQ+NKSRAIKAGII PL +LL+DKNLGM+D
Sbjct: 487 KGIPPLVLLLRDGTIRGKKDAATALFNLSLNQANKSRAIKAGIIQPLLALLEDKNLGMVD 546

Query: 553 EALSILLVLASNPEGRAEINNNSFIATLVNFIKVGTPKNKECATALLLELGLNNSPSILV 612
           EALSILL+LAS+PEGRAEI NNSFI  LVN I  GTPKNKECAT+LLLELG NNSPSILV
Sbjct: 547 EALSILLLLASHPEGRAEIGNNSFIEILVNIIIDGTPKNKECATSLLLELGRNNSPSILV 606

Query: 613 ALQFGVYEHLVEITKSGTSRAQRKANSLLQYMIKCEHI 647
           ALQFGVYEHLVE+T+ GTSRAQRKA SLLQYM KCEHI
Sbjct: 607 ALQFGVYEHLVELTRCGTSRAQRKATSLLQYMSKCEHI 644

BLAST of Sed0001098 vs. ExPASy TrEMBL
Match: A0A5A7U2R4 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold133G001050 PE=4 SV=1)

HSP 1 Score: 1006.1 bits (2600), Expect = 6.5e-290
Identity = 521/638 (81.66%), Postives = 579/638 (90.75%), Query Frame = 0

Query: 13  TNDSSVCSLIPNG----SEDMGKNLIELNELIESIAGFSGFRKTQSKECLNLVRRLKMLA 72
           +N  S CS I NG    + D+ K L E NELIE+I  FSGFRKTQSKECLNLVRRLKML 
Sbjct: 7   SNSLSPCSSISNGVLQETVDVTKCLAEANELIEAIGAFSGFRKTQSKECLNLVRRLKMLV 66

Query: 73  PLLEEIRDLHDTVPAQALSPHLAHLKEALVMAKRLLKNCHNGSKIYLAFENEAVMARFHG 132
           PLLEEIRDLHD +PA+ALS H++ LKEALV+AKRLLKNCHNGSKIYLAFENEAVMARFH 
Sbjct: 67  PLLEEIRDLHDMLPAEALSSHISLLKEALVLAKRLLKNCHNGSKIYLAFENEAVMARFHA 126

Query: 133 VYDKLKEALDELPYDKLGVSIELQEQVELISTQLKRAKCRRDTQDMELAMDMIVVFSKND 192
           VYDKLKEALD +PYD+LGVS+EL+EQVEL+STQLKRAKCR+DTQDMELAMDM+VVFSKND
Sbjct: 127 VYDKLKEALDGIPYDELGVSVELKEQVELMSTQLKRAKCRKDTQDMELAMDMMVVFSKND 186

Query: 193 ERNADPVILERLANKLELRKIADLEEETIAVQELIKRRCPPNSESVQQILDLLSKFKQIA 252
           ERNADPVILERLANKLELRKIADLE ETIAVQ+LI+ R  PNSES+QQI+DLL KFKQIA
Sbjct: 187 ERNADPVILERLANKLELRKIADLEAETIAVQKLIRHRGVPNSESLQQIIDLLCKFKQIA 246

Query: 253 GMNSNVVPDGPVALKRLQRCRSTLIPHEFLCPISLEIMSDPVIVATGQTYDRESIQQWLD 312
           GM++NV  DGPV  K L+RC+STLIP+EFLCPI+LEIM+DPVIVATGQTYDRESIQ+WL+
Sbjct: 247 GMDNNVALDGPVVSKSLRRCQSTLIPNEFLCPITLEIMTDPVIVATGQTYDRESIQKWLN 306

Query: 313 ANNRTCPKTGQTLMHLSLAPNYALKNLIVQWSQKSDQELPKKKVLAALGDTPPELTQEIS 372
           +N+RTCPKTGQTL+HLSLAPNYALKNLI+QW QK++  LPKK+V+A +GDTPP+L  EIS
Sbjct: 307 SNHRTCPKTGQTLVHLSLAPNYALKNLILQWCQKNNYNLPKKEVVAGMGDTPPDLAPEIS 366

Query: 373 LVLNNLSSSQLDVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV 432
            +++NLSSSQLD+QREAIIKIRVL+KENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV
Sbjct: 367 SLVHNLSSSQLDIQREAIIKIRVLSKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV 426

Query: 433 TALLNLSIDEANKVLIVKEGAIPAIIEILQHGTEEAKENSAATLFSLSMLEENKVLIGSL 492
           TALLNLSID+ANK LI +EGAIPAIIEILQ GTEEAKENSAA LFSLSML+ENKVLIGSL
Sbjct: 427 TALLNLSIDDANKRLIAREGAIPAIIEILQCGTEEAKENSAAALFSLSMLDENKVLIGSL 486

Query: 493 NGIPPLVSLLRDGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMID 552
            GIPPLV LLRDGT RGK+DAATALFNL+ NQ+NKSRAIKAGII PL +LL+DKNLGM+D
Sbjct: 487 KGIPPLVLLLRDGTIRGKKDAATALFNLSLNQANKSRAIKAGIIQPLLALLEDKNLGMVD 546

Query: 553 EALSILLVLASNPEGRAEINNNSFIATLVNFIKVGTPKNKECATALLLELGLNNSPSILV 612
           EALSILL+LAS+PEGRAEI NNSFI  LVN I  GTPKNKECAT+LLLELG NNSPSILV
Sbjct: 547 EALSILLLLASHPEGRAEIGNNSFIEILVNIIIDGTPKNKECATSLLLELGRNNSPSILV 606

Query: 613 ALQFGVYEHLVEITKSGTSRAQRKANSLLQYMIKCEHI 647
           ALQFGVYEHLVE+T+ GTSRAQRKA SLLQYM KCEHI
Sbjct: 607 ALQFGVYEHLVELTRCGTSRAQRKATSLLQYMSKCEHI 644

BLAST of Sed0001098 vs. ExPASy TrEMBL
Match: A0A6J1CFD0 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111011180 PE=4 SV=1)

HSP 1 Score: 1003.8 bits (2594), Expect = 3.2e-289
Identity = 521/651 (80.03%), Postives = 582/651 (89.40%), Query Frame = 0

Query: 1   MDNEIGVNAMANTNDSSVCSLIPNGSEDMG----KNLIELNELIESIAGFSGFRKTQSKE 60
           M+ E GV      N  S CS   +G  D G    K L E NELI +I GFSGFRKTQSKE
Sbjct: 79  MEREFGV---IGNNGWSRCSPASSGGRDEGDEVIKELTEANELIAAIGGFSGFRKTQSKE 138

Query: 61  CLNLVRRLKMLAPLLEEIRDLHDTVPAQALSPHLAHLKEALVMAKRLLKNCHNGSKIYLA 120
           CLNLVRRLKML PLLEEIRDLH+ VPA+ALSPHLAHLKEALV+AKRLLKNCHNGSKIYLA
Sbjct: 139 CLNLVRRLKMLVPLLEEIRDLHNMVPAEALSPHLAHLKEALVLAKRLLKNCHNGSKIYLA 198

Query: 121 FENEAVMARFHGVYDKLKEALDELPYDKLGVSIELQEQVELISTQLKRAKCRRDTQDMEL 180
           FENEAVMARFH VYDKLKEALD +PYD+LGVS+ELQEQVEL+STQLKRAKCR+DTQDMEL
Sbjct: 199 FENEAVMARFHAVYDKLKEALDGMPYDELGVSVELQEQVELMSTQLKRAKCRKDTQDMEL 258

Query: 181 AMDMIVVFSKNDERNADPVILERLANKLELRKIADLEEETIAVQELIKR-RCPPNSESVQ 240
           AMDM+VVFSKNDERNADPVILERLANKLELRKIADLE ET AVQ+L+KR R  P+SES+Q
Sbjct: 259 AMDMMVVFSKNDERNADPVILERLANKLELRKIADLEAETEAVQKLVKRSRGMPSSESLQ 318

Query: 241 QILDLLSKFKQIAGMNSNVVPDGPVALKRLQRCRSTLIPHEFLCPISLEIMSDPVIVATG 300
           QIL+LL KFK IAGM++++ P GPV+ K L+RCRS +IPHEFLCPI+LEIM+DPVIVATG
Sbjct: 319 QILELLGKFKGIAGMDNDIAPHGPVSSKCLRRCRSAVIPHEFLCPITLEIMTDPVIVATG 378

Query: 301 QTYDRESIQQWLDANNRTCPKTGQTLMHLSLAPNYALKNLIVQWSQKSDQELPKKKVLAA 360
           QTYDRESIQ+WLD+N+RTCPKTGQTL HLS+APNYALKNLI+QW QK+  ELPKK     
Sbjct: 379 QTYDRESIQKWLDSNHRTCPKTGQTLAHLSVAPNYALKNLILQWCQKNHYELPKKD--GG 438

Query: 361 LGDTPPELTQEISLVLNNLSSSQLDVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKL 420
           LGDTPPEL +EIS +++NLSSSQLDV++EAII IRVL+KENPENRVWIANSGVIPPLVKL
Sbjct: 439 LGDTPPELAREISSLVHNLSSSQLDVRKEAIINIRVLSKENPENRVWIANSGVIPPLVKL 498

Query: 421 LSYPDLNFQEHTVTALLNLSIDEANKVLIVKEGAIPAIIEILQHGTEEAKENSAATLFSL 480
           LSYPDL FQEHTVTALLNLSID++NK LI +EGAIPAIIEILQHGTEEAKENSAA LFSL
Sbjct: 499 LSYPDLKFQEHTVTALLNLSIDDSNKRLIAREGAIPAIIEILQHGTEEAKENSAAALFSL 558

Query: 481 SMLEENKVLIGSLNGIPPLVSLLRDGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPL 540
           SML+ENKVLIG+LNGIPPLV LLRDGT RGK+DAATALFNL+ NQSNKSRAIK GIIPPL
Sbjct: 559 SMLDENKVLIGTLNGIPPLVDLLRDGTIRGKKDAATALFNLSLNQSNKSRAIKGGIIPPL 618

Query: 541 RSLLKDKNLGMIDEALSILLVLASNPEGRAEINNNSFIATLVNFIKVGTPKNKECATALL 600
             LL+DK+LGM+DEALSILL+L S+PEGRAE+++NSFI T+VN +K GTPKNKECAT+LL
Sbjct: 619 LRLLEDKSLGMVDEALSILLLLTSHPEGRAELSHNSFIETIVNIMKDGTPKNKECATSLL 678

Query: 601 LELGLNNSPSILVALQFGVYEHLVEITKSGTSRAQRKANSLLQYMIKCEHI 647
           LELGLNNSPSILVALQ+GVYEHL+E+T+SGTSRAQRKANSLLQYM KCEHI
Sbjct: 679 LELGLNNSPSILVALQYGVYEHLIELTRSGTSRAQRKANSLLQYMSKCEHI 724

BLAST of Sed0001098 vs. ExPASy TrEMBL
Match: A0A5D3BSP7 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold388G00070 PE=4 SV=1)

HSP 1 Score: 996.5 bits (2575), Expect = 5.2e-287
Identity = 518/638 (81.19%), Postives = 576/638 (90.28%), Query Frame = 0

Query: 13  TNDSSVCSLIPNG----SEDMGKNLIELNELIESIAGFSGFRKTQSKECLNLVRRLKMLA 72
           +N  S CS I NG    + D+ K L E NELIE+I  FSGFRKTQSKECLNLVRRLKML 
Sbjct: 7   SNSLSPCSSISNGVLQETVDVTKCLAEANELIEAIGAFSGFRKTQSKECLNLVRRLKMLV 66

Query: 73  PLLEEIRDLHDTVPAQALSPHLAHLKEALVMAKRLLKNCHNGSKIYLAFENEAVMARFHG 132
           PLLEEIRDLHD +PA+ALS H++ LKEALV+AKRLLKNCHNGSKIYL   NEAVMARFH 
Sbjct: 67  PLLEEIRDLHDMLPAEALSSHISLLKEALVLAKRLLKNCHNGSKIYL---NEAVMARFHA 126

Query: 133 VYDKLKEALDELPYDKLGVSIELQEQVELISTQLKRAKCRRDTQDMELAMDMIVVFSKND 192
           VYDKLKEALD +PYD+LGVS+EL+EQVEL+STQLKRAKCR+DTQDMELAMDM+VVFSKND
Sbjct: 127 VYDKLKEALDGIPYDELGVSVELKEQVELMSTQLKRAKCRKDTQDMELAMDMMVVFSKND 186

Query: 193 ERNADPVILERLANKLELRKIADLEEETIAVQELIKRRCPPNSESVQQILDLLSKFKQIA 252
           ERNADPVILERLANKL+LRKIADLE ETIAVQ+LI+ R  PNSES+QQI+DLL KFKQIA
Sbjct: 187 ERNADPVILERLANKLDLRKIADLEAETIAVQKLIRHRGVPNSESLQQIIDLLCKFKQIA 246

Query: 253 GMNSNVVPDGPVALKRLQRCRSTLIPHEFLCPISLEIMSDPVIVATGQTYDRESIQQWLD 312
           GM++NV  DGPV  K L+RC+STLIPHEFLCPI+LEIM+DPVIVATGQTYDRESIQ+WL 
Sbjct: 247 GMDNNVALDGPVVSKSLRRCQSTLIPHEFLCPITLEIMTDPVIVATGQTYDRESIQKWLH 306

Query: 313 ANNRTCPKTGQTLMHLSLAPNYALKNLIVQWSQKSDQELPKKKVLAALGDTPPELTQEIS 372
           +N+RTCPKTGQTL+HLSLAPNYALKNLI+QW QK++  LPKK+V+A +GDTPP+L +EIS
Sbjct: 307 SNHRTCPKTGQTLVHLSLAPNYALKNLILQWCQKNNYNLPKKEVVAGMGDTPPDLAREIS 366

Query: 373 LVLNNLSSSQLDVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV 432
            +++NLSSSQLD+QREAIIKIRVL+KENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV
Sbjct: 367 SLVHNLSSSQLDIQREAIIKIRVLSKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTV 426

Query: 433 TALLNLSIDEANKVLIVKEGAIPAIIEILQHGTEEAKENSAATLFSLSMLEENKVLIGSL 492
           TALLNLSID+ANK LI +EGAIPAIIEILQ GTEEAKENSAA LFSLSML+ENKVLIGSL
Sbjct: 427 TALLNLSIDDANKRLIAREGAIPAIIEILQCGTEEAKENSAAALFSLSMLDENKVLIGSL 486

Query: 493 NGIPPLVSLLRDGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMID 552
            GIPPLV LLRDGT RGK+DAATALFNL+ NQ+NKSRAIKAGII PL +LL+DKNLGM+D
Sbjct: 487 KGIPPLVLLLRDGTIRGKKDAATALFNLSLNQANKSRAIKAGIIQPLLALLEDKNLGMVD 546

Query: 553 EALSILLVLASNPEGRAEINNNSFIATLVNFIKVGTPKNKECATALLLELGLNNSPSILV 612
           EALSILL+LAS+PEGRAEI NNSFI  LVN I  GTPKNKECAT+LLLELG NNSPSILV
Sbjct: 547 EALSILLLLASHPEGRAEIGNNSFIEILVNIIIDGTPKNKECATSLLLELGRNNSPSILV 606

Query: 613 ALQFGVYEHLVEITKSGTSRAQRKANSLLQYMIKCEHI 647
           ALQFGVYEHLVE+T+ GTSRAQRKA SLLQYM KCEHI
Sbjct: 607 ALQFGVYEHLVELTRCGTSRAQRKATSLLQYMSKCEHI 641

BLAST of Sed0001098 vs. TAIR 10
Match: AT5G42340.1 (Plant U-Box 15 )

HSP 1 Score: 686.0 bits (1769), Expect = 2.9e-197
Identity = 360/612 (58.82%), Postives = 466/612 (76.14%), Query Frame = 0

Query: 35  ELNELIESIAGFSGFRKTQSKECLNLVRRLKMLAPLLEEIRDLHDTVPAQALSPHLAHLK 94
           ++  ++E +   +G+R+TQ KEC NLVRRLK+L P L+EIR       + +    L  L+
Sbjct: 56  DIVSIVEFLDQINGYRRTQQKECFNLVRRLKILIPFLDEIRGFE----SPSCKHFLNRLR 115

Query: 95  EALVMAKRLLKNCHNGSKIYLAFENEAVMARFHGVYDKLKEALDELPYDKLGVSIELQEQ 154
           +  + AK+LL+ C NGSKIY+A + E +M RFH +Y+KL   L + P+D+L +S + +++
Sbjct: 116 KVFLAAKKLLETCSNGSKIYMALDGETMMTRFHSIYEKLNRVLVKAPFDELMISGDAKDE 175

Query: 155 VELISTQLKRAKCRRDTQDMELAMDMIVVFSKNDERNADPVILERLANKLELRKIADLEE 214
           ++ +  QLK+AK R DTQD+ELA+DM+VVFSK D RNAD  I+ERLA KLEL+ I DL+ 
Sbjct: 176 IDSLCKQLKKAKRRTDTQDIELAVDMMVVFSKTDPRNADSAIIERLAKKLELQTIDDLKT 235

Query: 215 ETIAVQELIKRRCPPNSESVQQILDLLSKFKQIAGMNSNVVPDGPVALKRLQRCRSTLIP 274
           ETIA+Q LI+ +   N E+ Q I++LL+KFK++ G+ +  +   PV  K + +  S ++P
Sbjct: 236 ETIAIQSLIQDKGGLNIETKQHIIELLNKFKKLQGLEATDILYQPVINKAITKSTSLILP 295

Query: 275 HEFLCPISLEIMSDPVIVATGQTYDRESIQQWLDANNRTCPKTGQTLMHLSLAPNYALKN 334
           HEFLCPI+LEIM DPVI+ATGQTY++ESIQ+W DA ++TCPKT Q L HLSLAPN+ALKN
Sbjct: 296 HEFLCPITLEIMLDPVIIATGQTYEKESIQKWFDAGHKTCPKTRQELDHLSLAPNFALKN 355

Query: 335 LIVQWSQKSDQELPKKKVLAALGDTPPELTQEISLVLNNLSSSQLDVQREAIIKIRVLAK 394
           LI+QW +K++ ++P+K+V     D+  E   E+SL++  LSSSQL+ QR ++ ++R+LA+
Sbjct: 356 LIMQWCEKNNFKIPEKEVSP---DSQNEQKDEVSLLVEALSSSQLEEQRRSVKQMRLLAR 415

Query: 395 ENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTVTALLNLSIDEANKVLIVKEGAIPAII 454
           ENPENRV IAN+G IP LV+LLSYPD   QE+ VT LLNLSIDE NK LI  EGAIP II
Sbjct: 416 ENPENRVLIANAGAIPLLVQLLSYPDSGIQENAVTTLLNLSIDEVNKKLISNEGAIPNII 475

Query: 455 EILQHGTEEAKENSAATLFSLSMLEENKVLIGSLNGIPPLVSLLRDGTFRGKRDAATALF 514
           EIL++G  EA+ENSAA LFSLSML+ENKV IG  NGIPPLV LL+ GT RGK+DA TALF
Sbjct: 476 EILENGNREARENSAAALFSLSMLDENKVTIGLSNGIPPLVDLLQHGTLRGKKDALTALF 535

Query: 515 NLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMIDEALSILLVLASNPEGRAEINNNSFIA 574
           NL+ N +NK RAI AGI+ PL +LLKDKNLGMIDEALSILL+LAS+PEGR  I   SFI 
Sbjct: 536 NLSLNSANKGRAIDAGIVQPLLNLLKDKNLGMIDEALSILLLLASHPEGRQAIGQLSFIE 595

Query: 575 TLVNFIKVGTPKNKECATALLLELGLNNSPSILVALQFGVYEHLVEITKSGTSRAQRKAN 634
           TLV FI+ GTPKNKECAT++LLELG NNS  IL ALQFGVYE+LVEIT SGT+RAQRKAN
Sbjct: 596 TLVEFIRQGTPKNKECATSVLLELGSNNSSFILAALQFGVYEYLVEITTSGTNRAQRKAN 655

Query: 635 SLLQYMIKCEHI 647
           +L+Q + K E I
Sbjct: 656 ALIQLISKSEQI 660

BLAST of Sed0001098 vs. TAIR 10
Match: AT3G46510.1 (plant U-box 13 )

HSP 1 Score: 473.0 bits (1216), Expect = 3.8e-133
Identity = 272/619 (43.94%), Postives = 395/619 (63.81%), Query Frame = 0

Query: 36  LNELIESIAGFSGFRKTQSKECLNLVRRLKMLAPLLEEIRDLHDTVPAQALSPHLAHLKE 95
           L +++  IA  S +R T  K C NL RRLK+L P+ EEIR+ ++ +    L   L +LKE
Sbjct: 12  LIDVVNEIAAISDYRITVKKLCYNLARRLKLLVPMFEEIRESNEPISEDTLKT-LMNLKE 71

Query: 96  ALVMAKRLLKNCHNGSKIYLAFENEAVMARFHGVYDKLKEALDELPYDKLGVSIELQEQV 155
           A+  AK  LK C  GSKIYL  E E V ++   V  KL+++L ++PY++L +S E++EQV
Sbjct: 72  AMCSAKDYLKFCSQGSKIYLVMEREQVTSKLMEVSVKLEQSLSQIPYEELDISDEVREQV 131

Query: 156 ELISTQLKRAKCRRDTQDMELAMDMIVVFSKNDERNADPVILERLANKLELRKIADLEEE 215
           EL+ +Q +RAK R D  D EL  D+  + +K+ + +A   +LER+A KL L +I DL +E
Sbjct: 132 ELVLSQFRRAKGRVDVSDDELYEDLQSLCNKSSDVDAYQPVLERVAKKLHLMEIPDLAQE 191

Query: 216 TIAVQELIKRR---CPPNSESVQQILDLLSKF---------KQIAGMNSNVVPDGPVALK 275
           ++A+ E++         N E +  +L ++  F         +Q  G+NS    +G  +  
Sbjct: 192 SVALHEMVASSGGDVGENIEEMAMVLKMIKDFVQTEDDNGEEQKVGVNSR--SNGQTSTA 251

Query: 276 RLQRCRSTLIPHEFLCPISLEIMSDPVIVATGQTYDRESIQQWLDANNRTCPKTGQTLMH 335
             Q+    +IP +F CPISLE+M DPVIV++GQTY+R  I++W++  + TCPKT Q L  
Sbjct: 252 ASQKI--PVIPDDFRCPISLEMMRDPVIVSSGQTYERTCIEKWIEGGHSTCPKTQQALTS 311

Query: 336 LSLAPNYALKNLIVQWSQKSDQELPKK----KVLAALGDTPPELTQEISLVLNNLSSSQL 395
            +L PNY L++LI QW + +D E PK     +       + P    +I  ++  L+    
Sbjct: 312 TTLTPNYVLRSLIAQWCEANDIEPPKPPSSLRPRKVSSFSSPAEANKIEDLMWRLAYGNP 371

Query: 396 DVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTVTALLNLSIDEA 455
           + QR A  +IR+LAK N +NRV IA +G IP LV LLS PD   QEH+VTALLNLSI E 
Sbjct: 372 EDQRSAAGEIRLLAKRNADNRVAIAEAGAIPLLVGLLSTPDSRIQEHSVTALLNLSICEN 431

Query: 456 NKVLIVKEGAIPAIIEILQHGTEEAKENSAATLFSLSMLEENKVLIGSLNGIPPLVSLLR 515
           NK  IV  GAIP I+++L+ G+ EA+EN+AATLFSLS+++ENKV IG+L  IPPLV LL 
Sbjct: 432 NKGAIVSAGAIPGIVQVLKKGSMEARENAAATLFSLSVIDENKVTIGALGAIPPLVVLLN 491

Query: 516 DGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMIDEALSILLVLAS 575
           +GT RGK+DAATALFNL   Q NK +AI+AG+IP L  LL +   GM+DEAL+IL +L+S
Sbjct: 492 EGTQRGKKDAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEPGSGMVDEALAILAILSS 551

Query: 576 NPEGRAEINNNSFIATLVNFIKVGTPKNKECATALLLELGLNNSPSILVALQFGVYEHLV 635
           +PEG+A I ++  + +LV FI+ G+P+N+E A A+L+ L   +   ++ A + G+   L+
Sbjct: 552 HPEGKAIIGSSDAVPSLVEFIRTGSPRNRENAAAVLVHLCSGDPQHLVEAQKLGLMGPLI 611

Query: 636 EITKSGTSRAQRKANSLLQ 639
           ++  +GT R +RKA  LL+
Sbjct: 612 DLAGNGTDRGKRKAAQLLE 625

BLAST of Sed0001098 vs. TAIR 10
Match: AT3G54850.1 (plant U-box 14 )

HSP 1 Score: 451.4 bits (1160), Expect = 1.2e-126
Identity = 262/618 (42.39%), Postives = 396/618 (64.08%), Query Frame = 0

Query: 36  LNELIESIAGFSGFRKTQSKECLNLVRRLKMLAPLLEEIRDLHDTVPAQALSPHLAHLKE 95
           L + ++ I+GFS  R    K   +LVRR+ +L+P  EE+ D++  +    ++   A ++ 
Sbjct: 16  LVDSVKEISGFSSSRGFIGKIQGDLVRRITLLSPFFEELIDVNVELKKDQITGFEA-MRI 75

Query: 96  ALVMAKRLLKNCHNGSKIYLAFENEAVMARFHGVYDKLKEALDELPYDKLGVSIELQEQV 155
           AL  +  L ++ + GSK++  F+ ++++ +F  +  +++ AL ++PY+K+ VS E++EQV
Sbjct: 76  ALDSSLELFRSVNGGSKLFQLFDRDSLVEKFRDMTVEIEAALSQIPYEKIEVSEEVREQV 135

Query: 156 ELISTQLKRAKCRRDTQDMELAMDMIVVFSKNDERNADPVILERLANKLELRKIADLEEE 215
           +L+  Q KRAK R +  D++L+ D+ +  +  D    DP+IL+RL+ +L+L  I +L++E
Sbjct: 136 QLLHFQFKRAKERWEESDLQLSHDLAMAENVMD---PDPIILKRLSQELQLTTIDELKKE 195

Query: 216 TIAVQELIKRRCPPNSESVQQILDLLSKFKQIAGMNSNVVPDGPVALKRLQRCRSTLIPH 275
           + A+ E          +  +++  LL        M S+  PD     + + R RS +IP 
Sbjct: 196 SHAIHEYFLSYDGDPDDCFERMSSLLKNLVDFVTMESS-DPDPSTGSRIVSRHRSPVIPE 255

Query: 276 EFLCPISLEIMSDPVIVATGQTYDRESIQQWLDANNRTCPKTGQTLMHLSLAPNYALKNL 335
            F CPISLE+M DPVIV+TGQTY+R SIQ+WLDA ++TCPK+ +TL+H  L PNY LK+L
Sbjct: 256 YFRCPISLELMKDPVIVSTGQTYERSSIQKWLDAGHKTCPKSQETLLHAGLTPNYVLKSL 315

Query: 336 IVQWSQKSDQELPKKK------VLAALGDTPPELTQEISLVLNNLSSSQLDVQREAIIKI 395
           I  W + +  ELP+ +       +     +  + T  +SL L  L++   + QR A  ++
Sbjct: 316 IALWCESNGIELPQNQGSCRTTKIGGSSSSDCDRTFVLSL-LEKLANGTTEQQRAAAGEL 375

Query: 396 RVLAKENPENRVWIANSGVIPPLVKLLSYPDLNFQEHTVTALLNLSIDEANKVLIVKEGA 455
           R+LAK N +NRV IA +G IP LV+LLS PD   QEH+VTALLNLSI+E NK  IV  GA
Sbjct: 376 RLLAKRNVDNRVCIAEAGAIPLLVELLSSPDPRTQEHSVTALLNLSINEGNKGAIVDAGA 435

Query: 456 IPAIIEILQHGTEEAKENSAATLFSLSMLEENKVLIGSLNGIPPLVSLLRDGTFRGKRDA 515
           I  I+E+L++G+ EA+EN+AATLFSLS+++ENKV IG+   I  L+SLL +GT RGK+DA
Sbjct: 436 ITDIVEVLKNGSMEARENAAATLFSLSVIDENKVAIGAAGAIQALISLLEEGTRRGKKDA 495

Query: 516 ATALFNLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMIDEALSILLVLASNPEGRAEINN 575
           ATA+FNL   Q NKSRA+K GI+ PL  LLKD   GM+DEAL+IL +L++N EG+  I  
Sbjct: 496 ATAIFNLCIYQGNKSRAVKGGIVDPLTRLLKDAGGGMVDEALAILAILSTNQEGKTAIAE 555

Query: 576 NSFIATLVNFIKVGTPKNKECATALLLELGLNNSPSILVALQFGVYEHLVEITKSGTSRA 635
              I  LV  I+ G+P+N+E A A+L  L + N   + VA + G    L E+T++GT RA
Sbjct: 556 AESIPVLVEIIRTGSPRNRENAAAILWYLCIGNIERLNVAREVGADVALKELTENGTDRA 615

Query: 636 QRKANSLLQYMIKCEHIS 648
           +RKA SLL+ + + E ++
Sbjct: 616 KRKAASLLELIQQTEGVA 627

BLAST of Sed0001098 vs. TAIR 10
Match: AT2G28830.1 (PLANT U-BOX 12 )

HSP 1 Score: 430.3 bits (1105), Expect = 2.8e-120
Identity = 260/621 (41.87%), Postives = 390/621 (62.80%), Query Frame = 0

Query: 36  LNELIESIAGFSGFRKTQSKECLNLVRRLKMLAPLLEEIRDLHDTVPAQALSPHLAHLKE 95
           L + I  IA  S       K C NL RRL +L P+LEEIRD  ++  +  +   L  +K+
Sbjct: 13  LIDSINEIASISDSVTPMKKHCANLSRRLSLLLPMLEEIRDNQES--SSEVVNALLSVKQ 72

Query: 96  ALVMAKRLLKNCHNGSKIYLAFENEAVMARFHGVYDKLKEALDELPYDKLGVSIELQEQV 155
           +L+ AK LL    + SKIYL  E + VM +F  V   L++AL  +PY+ L +S EL+EQV
Sbjct: 73  SLLHAKDLLSFVSHVSKIYLVLERDQVMVKFQKVTSLLEQALSIIPYENLEISDELKEQV 132

Query: 156 ELISTQLKRAKCRR--DTQDMELAMDMIVVFS-KNDERNADPVILERLANKLELRKIADL 215
           EL+  QL+R+  +R  D  D EL  D++ ++S +     +D  ++ R+A KL+L  I DL
Sbjct: 133 ELVLVQLRRSLGKRGGDVYDDELYKDVLSLYSGRGSVMESD--MVRRVAEKLQLMTITDL 192

Query: 216 EEETIAVQELIKRRCPPN-SESVQQILDLLSKFKQIAGMNSNVVPDGPVALK-RLQRCRS 275
            +E++A+ +++      +  ES +++  +L K K      +  + D P+ LK  L + R 
Sbjct: 193 TQESLALLDMVSSSGGDDPGESFEKMSMVLKKIKDFVQTYNPNLDDAPLRLKSSLPKSRD 252

Query: 276 -----TLIPHEFLCPISLEIMSDPVIVATGQTYDRESIQQWLDANNRTCPKTGQTLMHLS 335
                 + P EF CPISLE+M+DPVIV++GQTY+RE I++WL+  + TCPKT +TL    
Sbjct: 253 DDRDMLIPPEEFRCPISLELMTDPVIVSSGQTYERECIKKWLEGGHLTCPKTQETLTSDI 312

Query: 336 LAPNYALKNLIVQWSQKSDQELPKKKVL-------AALGDTPPELTQEISLVLNNLSSSQ 395
           + PNY L++LI QW + +  E PK+  +       ++    P +   +I  +L  L+S Q
Sbjct: 313 MTPNYVLRSLIAQWCESNGIEPPKRPNISQPSSKASSSSSAPDDEHNKIEELLLKLTSQQ 372

Query: 396 LDVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKLLSYP-DLNFQEHTVTALLNLSID 455
            + +R A  +IR+LAK+N  NRV IA SG IP LV LL+   D   QEH VT++LNLSI 
Sbjct: 373 PEDRRSAAGEIRLLAKQNNHNRVAIAASGAIPLLVNLLTISNDSRTQEHAVTSILNLSIC 432

Query: 456 EANKVLIV-KEGAIPAIIEILQHGTEEAKENSAATLFSLSMLEENKVLIGSLNGIPPLVS 515
           + NK  IV   GA+P I+ +LQ G+ EA+EN+AATLFSLS+++ENKV IG+   IPPLV+
Sbjct: 433 QENKGKIVYSSGAVPGIVHVLQKGSMEARENAAATLFSLSVIDENKVTIGAAGAIPPLVT 492

Query: 516 LLRDGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPLRSLLKDKNLGMIDEALSILLV 575
           LL +G+ RGK+DAATALFNL   Q NK +A++AG++P L  LL +   GM+DE+LSIL +
Sbjct: 493 LLSEGSQRGKKDAATALFNLCIFQGNKGKAVRAGLVPVLMRLLTEPESGMVDESLSILAI 552

Query: 576 LASNPEGRAEINNNSFIATLVNFIKVGTPKNKECATALLLELGLNNSPSILVALQFGVYE 635
           L+S+P+G++E+     +  LV+FI+ G+P+NKE + A+L+ L   N   ++ A + G+ +
Sbjct: 553 LSSHPDGKSEVGAADAVPVLVDFIRSGSPRNKENSAAVLVHLCSWNQQHLIEAQKLGIMD 612

Query: 636 HLVEITKSGTSRAQRKANSLL 638
            L+E+ ++GT R +RKA  LL
Sbjct: 613 LLIEMAENGTDRGKRKAAQLL 629

BLAST of Sed0001098 vs. TAIR 10
Match: AT1G71020.1 (ARM repeat superfamily protein )

HSP 1 Score: 345.1 bits (884), Expect = 1.2e-94
Identity = 239/630 (37.94%), Postives = 361/630 (57.30%), Query Frame = 0

Query: 32  NLIELNELIESIAGFSGFRKTQSKECLNLVRRLKMLAPLLEEIRDL---HDTVPAQALSP 91
           +LI L   I  I G  G  K   K+C +L RR+ +L  L+EEIRD     ++  + +L+ 
Sbjct: 10  SLIGLIAEINEIPGNFGLFK---KDCSDLARRVGLLTHLIEEIRDSSPPSESDASSSLNS 69

Query: 92  HLAHLKEALVMAKRLLKNCHNGSKIYLAFEN-----EAVMARFHGVYDKLKEALDELPYD 151
           H       LV+  +  K   + +  + A E+     + +  +F  V  KL++AL +L YD
Sbjct: 70  HECDWWSDLVVGLQAAKRLLSSATSFQARESSDGAAKRISFQFQCVTWKLEKALGDLTYD 129

Query: 152 KLGVSIELQEQVELISTQLKRAKCRRDTQDMELAMDMIVVFSKNDERNADPVILERLANK 211
           +  +S E++EQVEL   QL+RA  R  + + +          K     ++P+  +  +N+
Sbjct: 130 RYDISDEVREQVELARLQLRRAMQRYGSLNSK----------KFSSGLSEPMEKDASSNR 189

Query: 212 LELRKIADLEEETIAVQELIKRRCPP--NSESVQQILDLLSKFKQIAGMNSNVVPDGPVA 271
             + K+  + E   ++ +  K   PP   S SV  +   LSK      +   V  +   +
Sbjct: 190 KVIEKLESIPETVHSLSDEKKFESPPPWKSSSV-SLAFFLSKDGDDERLEKAVTENSDDS 249

Query: 272 LKRLQRCRSTLIPHEFLCPISLEIMSDPVIVATGQTYDRESIQQWLDANNRTCPKTGQTL 331
               Q+  +  IP +FLCPISLE+M DP IV+TGQTY+R  IQ+W+D  N +CPKT Q L
Sbjct: 250 ----QKSDNLTIPEDFLCPISLELMKDPAIVSTGQTYERSFIQRWIDCGNLSCPKTQQKL 309

Query: 332 MHLSLAPNYALKNLIVQWSQKSDQELP-------KKKVLAALGDTPPELTQEISLVLNNL 391
            + +L PNY L++LI QW  K + E P        K    +  D   +++   +LV   L
Sbjct: 310 ENFTLTPNYVLRSLISQWCTKHNIEQPGGYMNGRTKNSDGSFRDLSGDMSAIRALVC-KL 369

Query: 392 SSSQLDVQREAIIKIRVLAKENPENRVWIANSGVIPPLVKLL-SYPDLNFQEHTVTALLN 451
           SS  ++ +R A+ +IR L+K + +NR+ IA +G IP LVKLL S  D   QE+ VT +LN
Sbjct: 370 SSQSIEDRRTAVSEIRSLSKRSTDNRILIAEAGAIPVLVKLLTSDGDTETQENAVTCILN 429

Query: 452 LSIDEANKVLIVKEGAIPAIIEILQHGTEEAKENSAATLFSLSMLEENKVLIGSLNGIPP 511
           LSI E NK LI+  GA+ +I+ +L+ G+ EA+EN+AATLFSLS+ +ENK++IG+   I  
Sbjct: 430 LSIYEHNKELIMLAGAVTSIVLVLRAGSMEARENAAATLFSLSLADENKIIIGASGAIMA 489

Query: 512 LVSLLRDGTFRGKRDAATALFNLTSNQSNKSRAIKAGIIPPLRSLLKDKNL-GMIDEALS 571
           LV LL+ G+ RGK+DAATALFNL   Q NK RA++AGI+ PL  +L D +   M DEAL+
Sbjct: 490 LVDLLQYGSVRGKKDAATALFNLCIYQGNKGRAVRAGIVKPLVKMLTDSSSERMADEALT 549

Query: 572 ILLVLASNPEGRAEINNNSFIATLVNFIKVGTPKNKECATALLLELGLNNSPSILVALQF 631
           IL VLASN   +  I   + I  L++ ++   P+N+E A A+LL L   ++  ++   + 
Sbjct: 550 ILSVLASNQVAKTAILRANAIPPLIDCLQKDQPRNRENAAAILLCLCKRDTEKLISIGRL 609

Query: 632 GVYEHLVEITKSGTSRAQRKANSLLQYMIK 643
           G    L+E+++ GT RA+RKANSLL+ + K
Sbjct: 610 GAVVPLMELSRDGTERAKRKANSLLELLRK 620

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038880222.15.8e-29380.28U-box domain-containing protein 15 [Benincasa hispida][more]
XP_004140059.22.1e-29081.50U-box domain-containing protein 15 [Cucumis sativus] >KAE8646827.1 hypothetical ... [more]
XP_008448190.14.6e-29081.66PREDICTED: U-box domain-containing protein 15 [Cucumis melo][more]
KAA0047841.11.3e-28981.66U-box domain-containing protein 15 [Cucumis melo var. makuwa][more]
XP_022140545.16.7e-28980.03U-box domain-containing protein 15 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q681N24.0e-19658.82U-box domain-containing protein 15 OS=Arabidopsis thaliana OX=3702 GN=PUB15 PE=2... [more]
Q9SNC65.4e-13243.94U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1... [more]
Q8VZ401.7e-12542.39U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1... [more]
Q5VRH97.0e-12444.54U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Q9ZV314.0e-11941.87U-box domain-containing protein 12 OS=Arabidopsis thaliana OX=3702 GN=PUB12 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0KE271.0e-29081.50RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G118880 PE... [more]
A0A1S3BIJ22.2e-29081.66RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103490456 PE=4 ... [more]
A0A5A7U2R46.5e-29081.66RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A6J1CFD03.2e-28980.03RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101118... [more]
A0A5D3BSP75.2e-28781.19RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
AT5G42340.12.9e-19758.82Plant U-Box 15 [more]
AT3G46510.13.8e-13343.94plant U-box 13 [more]
AT3G54850.11.2e-12642.39plant U-box 14 [more]
AT2G28830.12.8e-12041.87PLANT U-BOX 12 [more]
AT1G71020.11.2e-9437.94ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 519..559
e-value: 0.19
score: 20.8
coord: 560..600
e-value: 320.0
score: 1.6
coord: 396..436
e-value: 9.7E-5
score: 31.8
coord: 602..642
e-value: 430.0
score: 0.6
coord: 437..477
e-value: 6.1
score: 14.9
coord: 478..518
e-value: 0.036
score: 23.2
IPR000225ArmadilloPFAMPF00514Armcoord: 480..518
e-value: 7.9E-7
score: 28.9
coord: 396..435
e-value: 6.4E-9
score: 35.6
coord: 438..476
e-value: 1.9E-4
score: 21.3
coord: 519..558
e-value: 2.9E-4
score: 20.8
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 407..449
score: 13.1774
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 448..490
score: 8.9774
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 489..531
score: 10.3074
IPR003613U box domainSMARTSM00504Ubox_2coord: 276..339
e-value: 7.0E-31
score: 118.6
IPR003613U box domainPFAMPF04564U-boxcoord: 273..342
e-value: 3.7E-21
score: 75.1
IPR003613U box domainPROSITEPS51698U_BOXcoord: 272..346
score: 41.912693
IPR036537Adaptor protein Cbl, N-terminal domain superfamilyGENE3D1.20.930.20coord: 26..143
e-value: 2.2E-10
score: 42.4
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 262..354
e-value: 2.2E-28
score: 100.1
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 506..642
e-value: 1.5E-15
score: 59.1
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 358..504
e-value: 4.6E-34
score: 119.9
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 31..644
NoneNo IPR availablePANTHERPTHR23315:SF49RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 31..644
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 277..319
e-value: 1.35838E-25
score: 97.3042
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 273..347
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 367..641

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0001098.1Sed0001098.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007166 cell surface receptor signaling pathway
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity