Sed0001018 (gene) Chayote v1

Overview
NameSed0001018
Typegene
OrganismSechium edule (Chayote v1)
Descriptionreticulocalbin-2
LocationLG13: 291381 .. 294375 (+)
RNA-Seq ExpressionSed0001018
SyntenySed0001018
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAGGGCGGTGGTTTATGTTCTTACGGCCGCCGCCTTCGCCTTGTTCTTCGTATTTCCGCCGTCCAAATCTCGCGATCGGAACCATCACGGGCTAACTACTCGTCGCCTAGGTTACAAATTCCGAAACCCTAATTTTGATCCTCTCGTCGCGGAAATGGAAAGAAACGCCGAGGAAAGCGGATTAGGAAGCCGAGAAAGCGCGATTGGAGTTGGAAAAACTGCGATTCCCGATGATTACAGACGTTACCACGACGGAGAGAAATTGAACATTTCGTTGCGGCTGCTCGTGTTGTTTCCTCTGCTGGATAAATCCCCAAAAAACGGAGCGATCAGTTACGAGGAGCTGGGCGATTGGGTTACTGGCCAAGCCATTGACAGACTGAATTACAGAACGCGGAAACAATTGGAGTTCAGTGACAAGAATGGAGATGGCGCCATTTCGTTCCATGAATATCTGCCGCAGTTTACAGAGGAAGATATTGGTAAGCGAAGTTCAAGAAATTTTCATTTTCTATTCAATTTTCTTGCAAATTAGATGCTTCTTAGATGGAATTTTACTGCTAAGTAAGGGTCCACATAGTCCGGGTTGAATTGGATCATGAATTTTTTTTAACTAAACCCGAACTTTTGGATTGATCAACTAGCTAACTCGTTCAATTCTATATATTAAAGCAACCCAACTTAATGTAACCCATATATTTTTGGATGGATTGAGTTAGATTATTGGATTGTAATCCCTCTTTAAAGTCAAGCTCATAAGTTCATAACAAGTACATAGGTAAAAAAAAAAGATTTGCATGATATTAGACATACTCGAATTCAAGTTATTTATCTTGTTAGTGCATGCTTCCTACCAATATGTTAAAAATCTATCATATTAATCAATCTTACATATATATAATATTATTGTTAGTTTGAAATGAGTTAGATTGGGTTGAACCAAGCTAAAAAATGACCTATCCGACCAAACCTAAAGAAAATTACTTAATTGTAATCCAACCAAACTCAAGCTCAAAGAACATTATAATCCAATTCAACCCTTACAGTTTAGGTTGGGTAGTCTAGCTGTTTGGATTTTCGGCTTTTTTGTACATCCCTATCAGTAAGAATGATAGATCGGAACAAATGGGAAGGAATGTACAGAATCTGGAATGAAAATTATTGTTTTCTTGGTAACCAACAACATTCTGGGCCGGAAATTGAAATTCTTAACATTGTGAAACAGCAAGAAATGGAACTGAGCATGGAGAAGCTGGATGGTGGAAAAGGCAATTCACAAATGCCGATGTTGACTCAAATGGATTGCTGAATTTTGATGAACTGAAAGAGTAATGTGCCATTTTCCTTTTTTTACTACTTTACTACTTTTAACCTTTACTTCTTGTATTTTCGTATGAATCATTTTTGTCTTGTACCCTTGTAAATGTGTCTCATTTAGTCCTTAAAGTTAATTATCCAATGAAATTAGGAGGATAGAATTGATGTAGAAGCTTGCATGATGTTGCTACATTGACCATTCTGTTCTATATATCTTTTCTGGTTAGTTATAAGACATGCCCCTGAATTTTGGGATCAAGCTTTTCCTCTGCTGGGGTCTTGGTCTTGGTGCAGTAAGTTGTTACGTATGGGCGGTCGTCCCTCGTCGTAAAGTAATCCATGTGAAGCTTTTGGAAAGAGGGGTAGTCTTGTGTGTACCATAGCATTTCTGGTCAAACTCGCCCAACCCGACTAAGAAGAATCTGAAGAACCAAACATGACAAACCCATCTTTTTATTTCCGTTAAATTCGATGAATGTTTCAAAACTACCCATTAAGTAGAAAAGCCGTTAGACTGTTGAACAAAAAGTTGACCGTTCATTTTTAGACTTAATCTAGACAAATATCTTAGTCACCTTGTTTTCATCCAATGTTCTAAAGCATATCTATTCCAAATGTAGTTTTGAAACTTTTATAAAGGTCAAAGGTAATTTTGCAACTTTTGAAAAAAATGGGTGCTTTTTAATCAAAAGACAAAATTTATGGGTGTTTTTAATTTTTTTTTTTAATAAATAATTTAGGCTTAGCCGTTATTCGGCTGTTTCTCACTATTTGGCAGCGTTACCTTTTCTTTATTATTGAAGTGGATAATTAACCGTAAAAGACTAAATCTGGACATGTTTATGAAAGTACTTTGACTAAAATGAAAGGATGAAAATGTAAAGGACGATAAAGTACATAGACGAGAATTGTAGTTTAATTTTAGAAACAAGTGTTTACTGACAAGAACGGCATGAGATCTTTCTGTCCTGCAGTTTCCTGCACCCACAAGATAGCAGCAATTACCGAATACAAAATTGGCTCTTGAGAGAGAAAATGAAGTAACTTTTCTCCTTAGTCAAGCTATAAACTATTTTCATCTGGCATTGAAAAGCAAAGCATCAGCTTCTGTCTAATATATGCAGGAGCATGGACAATGACAGAGATGGAAAACTCAATTTTGATGAATTTCTCCACCATGCGTATAACACATACAGGAACTATATCGAATTCGAAACTCAAGGGGACAATGTACCGAGCGCCAAAGAGAAGTTTGATGAGCTTGATCTCGATGGGGACAAGTAAGTATTTGCTTGGCGGCAGTGAGTTATTTTTCTTCCTGATTCCAATTTGTTTGGTTGTTTGTAACATCAAAGAAGTATGTGCAGATTATTGACAGTGGATGAACTCAGGCCATTGTTTCAGTATTTTAATCCTGGAGAACTGTCTCATGCACAACATTACACAAGTCATTTGTTTAATGAGGTATTATTAGTAGTTGATTTGAAGAGATTAAGAAATTAAACAGGATGAATAAATTAATTGTATGCTGTTAATTAACACTGCAGGCTGATGATAACAAAGATGGCAACTTAACACTTGATGAGATGCTTAATAATGAGAATGTTTTCTACAGCACAATATATGACAATCACAATGGCAACTATGAAGACGATTACCGTGATGAACTTTGA

mRNA sequence

ATGGAGAGGGCGGTGGTTTATGTTCTTACGGCCGCCGCCTTCGCCTTGTTCTTCGTATTTCCGCCGTCCAAATCTCGCGATCGGAACCATCACGGGCTAACTACTCGTCGCCTAGGTTACAAATTCCGAAACCCTAATTTTGATCCTCTCGTCGCGGAAATGGAAAGAAACGCCGAGGAAAGCGGATTAGGAAGCCGAGAAAGCGCGATTGGAGTTGGAAAAACTGCGATTCCCGATGATTACAGACGTTACCACGACGGAGAGAAATTGAACATTTCGTTGCGGCTGCTCGTGTTGTTTCCTCTGCTGGATAAATCCCCAAAAAACGGAGCGATCAGTTACGAGGAGCTGGGCGATTGGGTTACTGGCCAAGCCATTGACAGACTGAATTACAGAACGCGGAAACAATTGGAGTTCAGTGACAAGAATGGAGATGGCGCCATTTCGTTCCATGAATATCTGCCGCAGTTTACAGAGGAAGATATTGCAAGAAATGGAACTGAGCATGGAGAAGCTGGATGGTGGAAAAGGCAATTCACAAATGCCGATGTTGACTCAAATGGATTGCTGAATTTTGATGAACTGAAAGATTTCCTGCACCCACAAGATAGCAGCAATTACCGAATACAAAATTGGCTCTTGAGAGAGAAAATGAAGAGCATGGACAATGACAGAGATGGAAAACTCAATTTTGATGAATTTCTCCACCATGCGTATAACACATACAGGAACTATATCGAATTCGAAACTCAAGGGGACAATGTACCGAGCGCCAAAGAGAAGTTTGATGAGCTTGATCTCGATGGGGACAAATTATTGACAGTGGATGAACTCAGGCCATTGTTTCAGTATTTTAATCCTGGAGAACTGTCTCATGCACAACATTACACAAGTCATTTGTTTAATGAGGCTGATGATAACAAAGATGGCAACTTAACACTTGATGAGATGCTTAATAATGAGAATGTTTTCTACAGCACAATATATGACAATCACAATGGCAACTATGAAGACGATTACCGTGATGAACTTTGA

Coding sequence (CDS)

ATGGAGAGGGCGGTGGTTTATGTTCTTACGGCCGCCGCCTTCGCCTTGTTCTTCGTATTTCCGCCGTCCAAATCTCGCGATCGGAACCATCACGGGCTAACTACTCGTCGCCTAGGTTACAAATTCCGAAACCCTAATTTTGATCCTCTCGTCGCGGAAATGGAAAGAAACGCCGAGGAAAGCGGATTAGGAAGCCGAGAAAGCGCGATTGGAGTTGGAAAAACTGCGATTCCCGATGATTACAGACGTTACCACGACGGAGAGAAATTGAACATTTCGTTGCGGCTGCTCGTGTTGTTTCCTCTGCTGGATAAATCCCCAAAAAACGGAGCGATCAGTTACGAGGAGCTGGGCGATTGGGTTACTGGCCAAGCCATTGACAGACTGAATTACAGAACGCGGAAACAATTGGAGTTCAGTGACAAGAATGGAGATGGCGCCATTTCGTTCCATGAATATCTGCCGCAGTTTACAGAGGAAGATATTGCAAGAAATGGAACTGAGCATGGAGAAGCTGGATGGTGGAAAAGGCAATTCACAAATGCCGATGTTGACTCAAATGGATTGCTGAATTTTGATGAACTGAAAGATTTCCTGCACCCACAAGATAGCAGCAATTACCGAATACAAAATTGGCTCTTGAGAGAGAAAATGAAGAGCATGGACAATGACAGAGATGGAAAACTCAATTTTGATGAATTTCTCCACCATGCGTATAACACATACAGGAACTATATCGAATTCGAAACTCAAGGGGACAATGTACCGAGCGCCAAAGAGAAGTTTGATGAGCTTGATCTCGATGGGGACAAATTATTGACAGTGGATGAACTCAGGCCATTGTTTCAGTATTTTAATCCTGGAGAACTGTCTCATGCACAACATTACACAAGTCATTTGTTTAATGAGGCTGATGATAACAAAGATGGCAACTTAACACTTGATGAGATGCTTAATAATGAGAATGTTTTCTACAGCACAATATATGACAATCACAATGGCAACTATGAAGACGATTACCGTGATGAACTTTGA

Protein sequence

MERAVVYVLTAAAFALFFVFPPSKSRDRNHHGLTTRRLGYKFRNPNFDPLVAEMERNAEESGLGSRESAIGVGKTAIPDDYRRYHDGEKLNISLRLLVLFPLLDKSPKNGAISYEELGDWVTGQAIDRLNYRTRKQLEFSDKNGDGAISFHEYLPQFTEEDIARNGTEHGEAGWWKRQFTNADVDSNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLHHAYNTYRNYIEFETQGDNVPSAKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHYTSHLFNEADDNKDGNLTLDEMLNNENVFYSTIYDNHNGNYEDDYRDEL
Homology
BLAST of Sed0001018 vs. NCBI nr
Match: XP_022141310.1 (reticulocalbin-2 [Momordica charantia])

HSP 1 Score: 519.6 bits (1337), Expect = 2.0e-143
Identity = 254/348 (72.99%), Postives = 288/348 (82.76%), Query Frame = 0

Query: 1   MERAVVYVLTAAAFALFFVFPPSKSRDRNHHGLTTRRLGYKFRNPNFDPLVAEMERNAEE 60
           M RAVVY+    AFA FFVF PS S DRNH  L TRRLGYK R P FDPLV EMER AEE
Sbjct: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQEL-TRRLGYKLRAPTFDPLVEEMERYAEE 60

Query: 61  SGLGSRESAIGVGKTA----IPDDYRRYHDGEKLNISLRLLVLFPLLDKSPKNGAISYEE 120
            G G   +AI   +T     +PD Y+ Y+D  +L ISLRLLVLFPLLD SPK+G ISYEE
Sbjct: 61  HGSGRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEE 120

Query: 121 LGDWVTGQAIDRLNYRTRKQLEFSDKNGDGAISFHEYLPQFTEEDIARNGTEHGEAGWWK 180
           L DW+  QA+ RLNYRTRK+L+F DKNGDGAISF EYLPQFTEEDIA+ GT HGEAGWWK
Sbjct: 121 LSDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWK 180

Query: 181 RQFTNADVDSNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLH 240
           RQFTNAD D NGLLNF+ELKDFLHP+DSSNYRIQNWLLREKMK MD DRDGKLNFDEFLH
Sbjct: 181 RQFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLH 240

Query: 241 HAYNTYRNYIEFETQGDNVPSAKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHY 300
           H ++ Y++YIEFETQGD+VP+A+EKFDELDLD D+LL+V+ELRPLFQY +PGELS+AQHY
Sbjct: 241 HVFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHY 300

Query: 301 TSHLFNEADDNKDGNLTLDEMLNNENVFYSTIYDNHNGNYEDDYRDEL 345
           TSHL  EADDNKDG L+LDEMLN+E VFY+++Y+  NG+YEDDY DEL
Sbjct: 301 TSHLIYEADDNKDGYLSLDEMLNHEYVFYNSVYEGRNGDYEDDYHDEL 347

BLAST of Sed0001018 vs. NCBI nr
Match: XP_011651314.1 (reticulocalbin-2 [Cucumis sativus] >KGN57652.1 hypothetical protein Csa_010748 [Cucumis sativus])

HSP 1 Score: 518.5 bits (1334), Expect = 4.5e-143
Identity = 256/347 (73.78%), Postives = 292/347 (84.15%), Query Frame = 0

Query: 1   MERAVVYVLTAAAFALFFVFPPSKSRDRNHHGLTTRRLGYKFRNPNFDPLVAEMER-NAE 60
           M +AVVYVLTA  FALFF+  PS   +R+H    TRRLG+KF NP FDPLV EMER  AE
Sbjct: 1   MGKAVVYVLTATVFALFFLISPSNFHNRSHQ-QATRRLGFKFPNPTFDPLVTEMERLAAE 60

Query: 61  ESGLGSRESAIGVGKT--AIPDDYRRYHDGEKLNISLRLLVLFPLLDKSPKNGAISYEEL 120
           E G    E+AIGV      I D YR Y+D  +LNISLRLLVLFPLLD SPK+G ISYEEL
Sbjct: 61  ERG----ENAIGVDNQNHKIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEEL 120

Query: 121 GDWVTGQAIDRLNYRTRKQLEFSDKNGDGAISFHEYLPQFTEEDIARNGTEHGEAGWWKR 180
            DW+ GQAI+RLNYRT KQLEF DKNGD AISFHEYLPQFTEEDIARN T +GEAGWW++
Sbjct: 121 SDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRK 180

Query: 181 QFTNADVDSNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLHH 240
           QFTNADVD+NGLL FDELKDFLHP+DSSNYRIQNWLL +KMK MD+D+DGKLNFDEFLHH
Sbjct: 181 QFTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHH 240

Query: 241 AYNTYRNYIEFETQGDNVPSAKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHYT 300
            Y+ Y+NYIEFETQG++VPSA+EKFDELDLD D++L+ +ELRPLFQY +PGE+S+AQHYT
Sbjct: 241 TYDIYKNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYT 300

Query: 301 SHLFNEADDNKDGNLTLDEMLNNENVFYSTIYDNHNGNYEDDYRDEL 345
           SHL NEADDNKDG LT+DEMLN+E VFYST+Y+N NG+YEDDY DEL
Sbjct: 301 SHLINEADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 342

BLAST of Sed0001018 vs. NCBI nr
Match: XP_023541445.1 (reticulocalbin-2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 516.2 bits (1328), Expect = 2.2e-142
Identity = 255/347 (73.49%), Postives = 293/347 (84.44%), Query Frame = 0

Query: 1   MERAVVYVLTAAAFALFFVFPPSKSRDRNHHGLTTRRLGYKFRNPNFDPLVAEMERNAEE 60
           M RAVVYVL  AAFA FFVF PS   DRN   L TRRLGYKFR P FDPLVAE+ER AEE
Sbjct: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQEL-TRRLGYKFRTPTFDPLVAELERIAEE 60

Query: 61  SGLGSRESAIGVGKTA---IPDDYRRYHDGEKLNISLRLLVLFPLLDKSPKNGAISYEEL 120
             LGSR + IGV +TA   IP+ YR  +D  +LNIS RL+VLFPL+D SPK+G I+YEE+
Sbjct: 61  RELGSRGNVIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEM 120

Query: 121 GDWVTGQAIDRLNYRTRKQLEFSDKNGDGAISFHEYLPQFTEEDIARNGTEHGEAGWWKR 180
            +W+T Q I++LN RT ++L F DKNGDGAISFHEYLPQFTEEDIARN T HG+AGWWK+
Sbjct: 121 SNWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQ 180

Query: 181 QFTNADVDSNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLHH 240
           QFTNADVD NGLL+FDELKDFLHPQDSSNYRIQNWLL EKMK MD DRDGKL+FDEFLHH
Sbjct: 181 QFTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHH 240

Query: 241 AYNTYRNYIEFETQGDNVPSAKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHYT 300
           AY+ Y++YIEFETQGD+VP+A+EKFDELDLD D++LTV+ELRPLFQY NPGELSHAQHY 
Sbjct: 241 AYDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYA 300

Query: 301 SHLFNEADDNKDGNLTLDEMLNNENVFYSTIYDNHNGNYEDDYRDEL 345
           +HL NEADDNKDG LTL+EML +E VFY+T+Y+N NG+YEDD+ DEL
Sbjct: 301 AHLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346

BLAST of Sed0001018 vs. NCBI nr
Match: XP_022945265.1 (reticulocalbin-2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 515.4 bits (1326), Expect = 3.8e-142
Identity = 254/347 (73.20%), Postives = 293/347 (84.44%), Query Frame = 0

Query: 1   MERAVVYVLTAAAFALFFVFPPSKSRDRNHHGLTTRRLGYKFRNPNFDPLVAEMERNAEE 60
           M RAVVYVL  AAFA FFVF PS   DRN   L TRRLGYKFR P FDPLVAE+ER AEE
Sbjct: 1   MGRAVVYVLILAAFAAFFVFSPSHLPDRNRQEL-TRRLGYKFRTPTFDPLVAELERIAEE 60

Query: 61  SGLGSRESAIGVGKTA---IPDDYRRYHDGEKLNISLRLLVLFPLLDKSPKNGAISYEEL 120
             LGSR +AIGV +TA   IP+ YR  +D  +LNIS RL+VLFPL+D SP +G I+YEE+
Sbjct: 61  RELGSRGNAIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPTDGLINYEEM 120

Query: 121 GDWVTGQAIDRLNYRTRKQLEFSDKNGDGAISFHEYLPQFTEEDIARNGTEHGEAGWWKR 180
            +W+T Q +++LN RT ++L F DKNGDGAISFHEYLPQFTEEDIARN T HG+AGWWK+
Sbjct: 121 SNWITEQTVNKLNNRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQ 180

Query: 181 QFTNADVDSNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLHH 240
           QFTNADVD NGLL+FDELKDFLHPQDSSNYRIQNWLL EKMK MD DRDGKL+FDEFLHH
Sbjct: 181 QFTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHH 240

Query: 241 AYNTYRNYIEFETQGDNVPSAKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHYT 300
           AY+ Y++YIEFETQGD+VP+A+EKFDELDLD D++LTV+ELRPLFQY NPGELSHAQHY 
Sbjct: 241 AYDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYA 300

Query: 301 SHLFNEADDNKDGNLTLDEMLNNENVFYSTIYDNHNGNYEDDYRDEL 345
           +HL NEADDNKDG LTL+EML +E VFY+T+Y+N NG+YEDD+ DEL
Sbjct: 301 AHLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346

BLAST of Sed0001018 vs. NCBI nr
Match: XP_022968155.1 (calumenin-B isoform X1 [Cucurbita maxima])

HSP 1 Score: 514.6 bits (1324), Expect = 6.5e-142
Identity = 256/347 (73.78%), Postives = 292/347 (84.15%), Query Frame = 0

Query: 1   MERAVVYVLTAAAFALFFVFPPSKSRDRNHHGLTTRRLGYKFRNPNFDPLVAEMERNAEE 60
           M RAVVYVL  AAFA FFVF PS   DRN   L TRRLGYKFR P FDPLVAE+ER AEE
Sbjct: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQEL-TRRLGYKFRTPTFDPLVAELERIAEE 60

Query: 61  SGLGSRESAIGVGKTA---IPDDYRRYHDGEKLNISLRLLVLFPLLDKSPKNGAISYEEL 120
             LGSR +AIGV +TA   IP+ YR  +D  +LNIS RL+VLFPL+D SPK+G I+YEE+
Sbjct: 61  RELGSRGNAIGVERTAKAKIPEAYRYSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEM 120

Query: 121 GDWVTGQAIDRLNYRTRKQLEFSDKNGDGAISFHEYLPQFTEEDIARNGTEHGEAGWWKR 180
            +W+T Q ID+LN RTR++L F DKNGDGAISFHEYLPQF+EEDIARN T HG+AGWWK+
Sbjct: 121 SNWITEQTIDKLNSRTRRELAFYDKNGDGAISFHEYLPQFSEEDIARNETGHGQAGWWKQ 180

Query: 181 QFTNADVDSNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLHH 240
           QFTNADVD NGLLNFDELKDFLHPQDSSNYRIQNWLL EKMK MD  RDGKL+FDEFLHH
Sbjct: 181 QFTNADVDPNGLLNFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYGRDGKLSFDEFLHH 240

Query: 241 AYNTYRNYIEFETQGDNVPSAKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHYT 300
           AYN Y++YIEFETQG +VP+A+EKFDELDLD D++LTV+ELRPLFQY NPGELSHAQHY 
Sbjct: 241 AYNIYKSYIEFETQGVDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYA 300

Query: 301 SHLFNEADDNKDGNLTLDEMLNNENVFYSTIYDNHNGNYEDDYRDEL 345
           ++L NEADDNKDG LTL+EML +E VFY+T+Y+N NG+YEDD  DEL
Sbjct: 301 AYLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDSHDEL 346

BLAST of Sed0001018 vs. ExPASy Swiss-Prot
Match: Q8BP92 (Reticulocalbin-2 OS=Mus musculus OX=10090 GN=Rcn2 PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 2.7e-13
Identity = 77/292 (26.37%), Postives = 133/292 (45.55%), Query Frame = 0

Query: 66  RESAIGVGKTAIPDDYRRYHDGEKLNISLRLLVLFPLLDKSPKNGAISYEELGDWVTGQA 125
           RE+ +GV +    D+Y +    E+     RL  +   +D S  +G ++  EL  W+    
Sbjct: 44  REALLGVQEDV--DEYVKLGHEEQ---QRRLQSIIKKID-SDSDGFLTENELSQWIQMSF 103

Query: 126 IDRLNYRTRKQLEFSDKNGDGAISFHEYLPQF------TEEDIARNGTEHGEAGWW---- 185
                   ++Q    DKN DGA+++ EY  Q        +E+ A + TE G         
Sbjct: 104 KHYAMQEAKQQFVEYDKNSDGAVTWDEYNIQMYDRVIDFDENTALDDTEEGSFRQLHLKD 163

Query: 186 KRQFTNADVDSNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFL 245
           K++F  A+ DS   L+ +E   F HP++     +  ++++E ++  D + DG ++ +EFL
Sbjct: 164 KKRFEKANQDSGPGLSLEEFIAFEHPEEVD--YMTEFVIQEALEEHDKNGDGFVSLEEFL 223

Query: 246 HHAYNTYRNYIEFETQGDNVPSAKEKF-DELDLDGDKLLTVDELRPLFQYFNPGELSHAQ 305
                 YR         + +   K++F ++ D D D  L   E   L  +  P     AQ
Sbjct: 224 ----GDYRRDPTANEDPEWILVEKDRFVNDYDKDNDGRLDPQE---LLSWVVPNNQGIAQ 283

Query: 306 HYTSHLFNEADDNKDGNLTLDEMLNNENVFYSTIYDNHNGNYEDD--YRDEL 345
               HL +E D N D  L+ +E+L N+++F ++   ++     DD  Y DEL
Sbjct: 284 EEALHLIDEMDLNSDKKLSEEEILENQDLFLTSEATDYGRQLHDDYFYHDEL 320

BLAST of Sed0001018 vs. ExPASy Swiss-Prot
Match: Q7ZUC2 (45 kDa calcium-binding protein OS=Danio rerio OX=7955 GN=sdf4 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 3.5e-13
Identity = 67/276 (24.28%), Postives = 131/276 (47.46%), Query Frame = 0

Query: 86  DGEKLNISLRLLVLFPLLDKSPKNGAISYEELGDWVTGQAIDRLNYRTRK-QLEFS--DK 145
           D E      +L+ +F  +D + K+ ++S +E+  W+  +  +      R+ +L F   D 
Sbjct: 87  DSEPRRNRKKLIEIFTKVDIN-KDRSVSAKEMQRWIMEKTEEHFQEAVRENKLSFRAVDP 146

Query: 146 NGDGAISFHEYLPQF------TEEDIAR-----------NGTEHGEAGWWKRQFTNADVD 205
           +GDG +++ EY  +F       E+++A              T+        R F   +  
Sbjct: 147 DGDGLVTWDEYRVKFLASKGLNEKEVAEKIKNNEELKVDEETQEVLESLKDRWFQADNPP 206

Query: 206 SNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLHHAYNTYRNY 265
           ++ LLN +E   FLHP+ S    +  ++++E ++ +D D DGKL   EF+     T  N 
Sbjct: 207 ADQLLNEEEFLSFLHPEHSRG--MLRYMVKEIVRDLDQDGDGKLTLAEFISLPMGTVENQ 266

Query: 266 IEFETQGDNVPSAKEKFDE-LDLDGDKLLTVDELRPLFQYFNPGELSHAQHYTSHLFNEA 325
              +   D V   K++F+E +D + D ++T++EL    +Y +P    +A +    +   A
Sbjct: 267 QAQDIDDDWVRERKKEFEEVIDANHDTIVTMEELE---EYMDPMNEHNALNEAKQMIAVA 326

Query: 326 DDNKDGNLTLDEMLNNENVFYSTIYDNHNGNYEDDY 341
           D+N++ NL L+E+L     F  +   ++  N  +++
Sbjct: 327 DENQNHNLELEEILKYSEYFTGSKLMDYARNVHEEF 356

BLAST of Sed0001018 vs. ExPASy Swiss-Prot
Match: B5X4E0 (Calumenin-B OS=Salmo salar OX=8030 GN=calub PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 7.8e-13
Identity = 61/236 (25.85%), Postives = 119/236 (50.42%), Query Frame = 0

Query: 95  RLLVLFPLLDKSPKNGAISYEELGDWVTGQAIDRLNYRTRKQLEFSDKNGDGAISFHEY- 154
           RL +L   +D+  K+G +S EE+  W+       +     +Q +  D NGDG +S+ EY 
Sbjct: 73  RLGMLVERIDED-KDGYVSVEEMKKWIKHSQKRWIYDDVDRQWKGHDHNGDGLVSWEEYK 132

Query: 155 ---LPQFTEEDIARNGTEHGE-AGWWKRQFTNADVDSNGLLNFDELKDFLHPQDSSNYRI 214
                   ++    +G  + +     +R+F  +D+D++   N +E   FLHP++  +  +
Sbjct: 133 NATYGYILDDPDPEDGFSYRQMISRDERRFKMSDLDADLKANKEEFTAFLHPEEYDH--M 192

Query: 215 QNWLLREKMKSMDNDRDGKLNFDEFLHHAYNTYRNYIEFETQGDNVPSAKEKFDEL-DLD 274
           ++ ++ E M+ +D + DG ++ +E++   YN   +  E E     V + +E+F E  D +
Sbjct: 193 KDIVVLETMEDIDKNGDGFIDLEEYIGDMYNQEGDPSEPEW----VRTEREQFTEFRDTN 252

Query: 275 GDKLLTVDELRPLFQYFNPGELSHAQHYTSHLFNEADDNKDGNLTLDEMLNNENVF 325
            D  +  +E +    +  P +  HA+    HL  E+D++KDG LT  E++   ++F
Sbjct: 253 KDGRMDKEETK---DWILPSDYDHAEAEAKHLVYESDNDKDGKLTKAEIVEKYDLF 298

BLAST of Sed0001018 vs. ExPASy Swiss-Prot
Match: Q3T0K1 (Calumenin OS=Bos taurus OX=9913 GN=CALU PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.3e-12
Identity = 72/326 (22.09%), Postives = 146/326 (44.79%), Query Frame = 0

Query: 7   YVLTAAAFALFFVFPPSKSRDRNHHGLTTRRLGYKFRNPNFDPLVAEMERNAEESGLGSR 66
           +++  +    F +  P++ +DR HH                +P +++   N  +S     
Sbjct: 6   FLMCLSLCTAFALSKPTEKKDRVHH----------------EPQLSDKVHNDAQSFDYDH 65

Query: 67  ESAIGVGKTAIPDDYRRYHDGEKLNISLRLLVLFPLLDK--SPKNGAISYEELGDWVTGQ 126
           ++ +G  +    D        E+L +         ++DK  + K+G ++  EL  W+   
Sbjct: 66  DAFLGAEEAKTFDQLTPEESKERLGM---------IVDKIDADKDGFVTEGELKSWIKHA 125

Query: 127 AIDRLNYRTRKQLEFSDKNGDGAISFHEY----LPQFTEEDIARNGTEHGEAGWW-KRQF 186
               +      Q +  D N DG IS+ EY       + ++    +G  + +     +R+F
Sbjct: 126 QKKYIYDNVENQWQEFDLNQDGLISWDEYRNVTYGTYLDDPDPDDGFNYKQMMVRDERRF 185

Query: 187 TNADVDSNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLHHAY 246
             AD D + +   +E   FLHP++     +++ +++E M+ +D + DG ++ +E++   Y
Sbjct: 186 KMADKDGDLIATKEEFTAFLHPEEYD--YMKDIVVQEPMEDIDKNADGFIDLEEYIGDMY 245

Query: 247 NTYRNYIEFETQGDNVPSAKEKFDEL-DLDGDKLLTVDELRPLFQYFNPGELSHAQHYTS 306
           +   N  E E     V + +E+F E  D + D  +  +E +    +  P +  HA+    
Sbjct: 246 SHDGNADEPEW----VKTEREQFVEFRDKNRDGKMDKEETK---DWILPSDYDHAEAEAR 297

Query: 307 HLFNEADDNKDGNLTLDEMLNNENVF 325
           HL  E+D NKDG LT +E+++  ++F
Sbjct: 306 HLVYESDQNKDGKLTKEEIVDKYDLF 297

BLAST of Sed0001018 vs. ExPASy Swiss-Prot
Match: Q62703 (Reticulocalbin-2 OS=Rattus norvegicus OX=10116 GN=Rcn2 PE=1 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 1.3e-12
Identity = 76/292 (26.03%), Postives = 131/292 (44.86%), Query Frame = 0

Query: 66  RESAIGVGKTAIPDDYRRYHDGEKLNISLRLLVLFPLLDKSPKNGAISYEELGDWVTGQA 125
           RE+ +GV +    D+Y +    E+     RL  +   +D S  +G ++  EL  W+    
Sbjct: 44  RETLLGVQEDV--DEYVKLGHEEQ---QRRLQSIIKKID-SDSDGFLTENELSQWIQMSF 103

Query: 126 IDRLNYRTRKQLEFSDKNGDGAISFHEYLPQF------TEEDIARNGTEHGEAGWW---- 185
                   ++Q    DKN DG +++ EY  Q        +E+ A + TE           
Sbjct: 104 KHYAMQEAKQQFVEYDKNSDGTVTWDEYNVQMYDRVIDFDENTALDDTEEESFRQLHLKD 163

Query: 186 KRQFTNADVDSNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFL 245
           K++F  A+ DS   LN +E   F HP++     +  ++++E ++  D + DG ++ +EFL
Sbjct: 164 KKRFEKANQDSGPGLNLEEFIAFEHPEEVD--YMTEFVIQEALEEHDKNGDGFVSLEEFL 223

Query: 246 HHAYNTYRNYIEFETQGDNVPSAKEKF-DELDLDGDKLLTVDELRPLFQYFNPGELSHAQ 305
                 YR         + +   K++F ++ D D D  L   E   L  +  P     AQ
Sbjct: 224 ----GDYRRDPTANEDPEWILVEKDRFVNDYDKDSDGRLDPQE---LLSWVVPNNQGIAQ 283

Query: 306 HYTSHLFNEADDNKDGNLTLDEMLNNENVFYSTIYDNHNGNYEDD--YRDEL 345
               HL +E D N D  L+ +E+L N+++F ++   ++     DD  Y DEL
Sbjct: 284 EEALHLIDEMDLNSDKKLSEEEILENQDLFLTSEATDYGRQLHDDYFYHDEL 320

BLAST of Sed0001018 vs. ExPASy TrEMBL
Match: A0A6J1CHQ2 (reticulocalbin-2 OS=Momordica charantia OX=3673 GN=LOC111011736 PE=4 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 9.8e-144
Identity = 254/348 (72.99%), Postives = 288/348 (82.76%), Query Frame = 0

Query: 1   MERAVVYVLTAAAFALFFVFPPSKSRDRNHHGLTTRRLGYKFRNPNFDPLVAEMERNAEE 60
           M RAVVY+    AFA FFVF PS S DRNH  L TRRLGYK R P FDPLV EMER AEE
Sbjct: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQEL-TRRLGYKLRAPTFDPLVEEMERYAEE 60

Query: 61  SGLGSRESAIGVGKTA----IPDDYRRYHDGEKLNISLRLLVLFPLLDKSPKNGAISYEE 120
            G G   +AI   +T     +PD Y+ Y+D  +L ISLRLLVLFPLLD SPK+G ISYEE
Sbjct: 61  HGSGRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEE 120

Query: 121 LGDWVTGQAIDRLNYRTRKQLEFSDKNGDGAISFHEYLPQFTEEDIARNGTEHGEAGWWK 180
           L DW+  QA+ RLNYRTRK+L+F DKNGDGAISF EYLPQFTEEDIA+ GT HGEAGWWK
Sbjct: 121 LSDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWK 180

Query: 181 RQFTNADVDSNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLH 240
           RQFTNAD D NGLLNF+ELKDFLHP+DSSNYRIQNWLLREKMK MD DRDGKLNFDEFLH
Sbjct: 181 RQFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLH 240

Query: 241 HAYNTYRNYIEFETQGDNVPSAKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHY 300
           H ++ Y++YIEFETQGD+VP+A+EKFDELDLD D+LL+V+ELRPLFQY +PGELS+AQHY
Sbjct: 241 HVFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHY 300

Query: 301 TSHLFNEADDNKDGNLTLDEMLNNENVFYSTIYDNHNGNYEDDYRDEL 345
           TSHL  EADDNKDG L+LDEMLN+E VFY+++Y+  NG+YEDDY DEL
Sbjct: 301 TSHLIYEADDNKDGYLSLDEMLNHEYVFYNSVYEGRNGDYEDDYHDEL 347

BLAST of Sed0001018 vs. ExPASy TrEMBL
Match: A0A0A0LAH3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G238180 PE=4 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 2.2e-143
Identity = 256/347 (73.78%), Postives = 292/347 (84.15%), Query Frame = 0

Query: 1   MERAVVYVLTAAAFALFFVFPPSKSRDRNHHGLTTRRLGYKFRNPNFDPLVAEMER-NAE 60
           M +AVVYVLTA  FALFF+  PS   +R+H    TRRLG+KF NP FDPLV EMER  AE
Sbjct: 1   MGKAVVYVLTATVFALFFLISPSNFHNRSHQ-QATRRLGFKFPNPTFDPLVTEMERLAAE 60

Query: 61  ESGLGSRESAIGVGKT--AIPDDYRRYHDGEKLNISLRLLVLFPLLDKSPKNGAISYEEL 120
           E G    E+AIGV      I D YR Y+D  +LNISLRLLVLFPLLD SPK+G ISYEEL
Sbjct: 61  ERG----ENAIGVDNQNHKIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEEL 120

Query: 121 GDWVTGQAIDRLNYRTRKQLEFSDKNGDGAISFHEYLPQFTEEDIARNGTEHGEAGWWKR 180
            DW+ GQAI+RLNYRT KQLEF DKNGD AISFHEYLPQFTEEDIARN T +GEAGWW++
Sbjct: 121 SDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRK 180

Query: 181 QFTNADVDSNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLHH 240
           QFTNADVD+NGLL FDELKDFLHP+DSSNYRIQNWLL +KMK MD+D+DGKLNFDEFLHH
Sbjct: 181 QFTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHH 240

Query: 241 AYNTYRNYIEFETQGDNVPSAKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHYT 300
            Y+ Y+NYIEFETQG++VPSA+EKFDELDLD D++L+ +ELRPLFQY +PGE+S+AQHYT
Sbjct: 241 TYDIYKNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYT 300

Query: 301 SHLFNEADDNKDGNLTLDEMLNNENVFYSTIYDNHNGNYEDDYRDEL 345
           SHL NEADDNKDG LT+DEMLN+E VFYST+Y+N NG+YEDDY DEL
Sbjct: 301 SHLINEADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 342

BLAST of Sed0001018 vs. ExPASy TrEMBL
Match: A0A6J1G0G2 (reticulocalbin-2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449565 PE=4 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.9e-142
Identity = 254/347 (73.20%), Postives = 293/347 (84.44%), Query Frame = 0

Query: 1   MERAVVYVLTAAAFALFFVFPPSKSRDRNHHGLTTRRLGYKFRNPNFDPLVAEMERNAEE 60
           M RAVVYVL  AAFA FFVF PS   DRN   L TRRLGYKFR P FDPLVAE+ER AEE
Sbjct: 1   MGRAVVYVLILAAFAAFFVFSPSHLPDRNRQEL-TRRLGYKFRTPTFDPLVAELERIAEE 60

Query: 61  SGLGSRESAIGVGKTA---IPDDYRRYHDGEKLNISLRLLVLFPLLDKSPKNGAISYEEL 120
             LGSR +AIGV +TA   IP+ YR  +D  +LNIS RL+VLFPL+D SP +G I+YEE+
Sbjct: 61  RELGSRGNAIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPTDGLINYEEM 120

Query: 121 GDWVTGQAIDRLNYRTRKQLEFSDKNGDGAISFHEYLPQFTEEDIARNGTEHGEAGWWKR 180
            +W+T Q +++LN RT ++L F DKNGDGAISFHEYLPQFTEEDIARN T HG+AGWWK+
Sbjct: 121 SNWITEQTVNKLNNRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQ 180

Query: 181 QFTNADVDSNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLHH 240
           QFTNADVD NGLL+FDELKDFLHPQDSSNYRIQNWLL EKMK MD DRDGKL+FDEFLHH
Sbjct: 181 QFTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHH 240

Query: 241 AYNTYRNYIEFETQGDNVPSAKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHYT 300
           AY+ Y++YIEFETQGD+VP+A+EKFDELDLD D++LTV+ELRPLFQY NPGELSHAQHY 
Sbjct: 241 AYDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYA 300

Query: 301 SHLFNEADDNKDGNLTLDEMLNNENVFYSTIYDNHNGNYEDDYRDEL 345
           +HL NEADDNKDG LTL+EML +E VFY+T+Y+N NG+YEDD+ DEL
Sbjct: 301 AHLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346

BLAST of Sed0001018 vs. ExPASy TrEMBL
Match: A0A6J1HWE3 (calumenin-B isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467475 PE=4 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 3.2e-142
Identity = 256/347 (73.78%), Postives = 292/347 (84.15%), Query Frame = 0

Query: 1   MERAVVYVLTAAAFALFFVFPPSKSRDRNHHGLTTRRLGYKFRNPNFDPLVAEMERNAEE 60
           M RAVVYVL  AAFA FFVF PS   DRN   L TRRLGYKFR P FDPLVAE+ER AEE
Sbjct: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQEL-TRRLGYKFRTPTFDPLVAELERIAEE 60

Query: 61  SGLGSRESAIGVGKTA---IPDDYRRYHDGEKLNISLRLLVLFPLLDKSPKNGAISYEEL 120
             LGSR +AIGV +TA   IP+ YR  +D  +LNIS RL+VLFPL+D SPK+G I+YEE+
Sbjct: 61  RELGSRGNAIGVERTAKAKIPEAYRYSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEM 120

Query: 121 GDWVTGQAIDRLNYRTRKQLEFSDKNGDGAISFHEYLPQFTEEDIARNGTEHGEAGWWKR 180
            +W+T Q ID+LN RTR++L F DKNGDGAISFHEYLPQF+EEDIARN T HG+AGWWK+
Sbjct: 121 SNWITEQTIDKLNSRTRRELAFYDKNGDGAISFHEYLPQFSEEDIARNETGHGQAGWWKQ 180

Query: 181 QFTNADVDSNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLHH 240
           QFTNADVD NGLLNFDELKDFLHPQDSSNYRIQNWLL EKMK MD  RDGKL+FDEFLHH
Sbjct: 181 QFTNADVDPNGLLNFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYGRDGKLSFDEFLHH 240

Query: 241 AYNTYRNYIEFETQGDNVPSAKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHYT 300
           AYN Y++YIEFETQG +VP+A+EKFDELDLD D++LTV+ELRPLFQY NPGELSHAQHY 
Sbjct: 241 AYNIYKSYIEFETQGVDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYA 300

Query: 301 SHLFNEADDNKDGNLTLDEMLNNENVFYSTIYDNHNGNYEDDYRDEL 345
           ++L NEADDNKDG LTL+EML +E VFY+T+Y+N NG+YEDD  DEL
Sbjct: 301 AYLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDSHDEL 346

BLAST of Sed0001018 vs. ExPASy TrEMBL
Match: A0A5D3CJD2 (Reticulocalbin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001480 PE=4 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 9.2e-142
Identity = 254/348 (72.99%), Postives = 292/348 (83.91%), Query Frame = 0

Query: 1   MERAVVYVLTAAAFALFFVFPPSKSRDRNHHGLTTRRLGYKFRNPNFDPLVAEMER-NAE 60
           M +AVVYVLTA AFA+FF+  PS   +RN+    TRRLG+KF NP FDPLV EMER  AE
Sbjct: 1   MGKAVVYVLTATAFAVFFLISPSNFHNRNYQ-QPTRRLGFKFPNPTFDPLVTEMERLEAE 60

Query: 61  ESGLGSRESAIGVGKT--AIPDDYRRYHDGEKLNISLRLLVLFPLLDKSPKNGAISYEEL 120
           E G    E+AIG+      I D YR Y+D  +LNISLRLLVLFPLLD SPK+G ISYEEL
Sbjct: 61  ERG----ENAIGMENQNHKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEEL 120

Query: 121 GDWVTGQAIDRLNYRTRKQLEFSDKNGDGAISFHEYLPQFTEEDIARNGTEHGEAGWWKR 180
            DW+ GQAI+RLNYRT KQLEF DKNGD AISFHEYLPQFT+EDIARN T +GEAGWW +
Sbjct: 121 SDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIK 180

Query: 181 QFTNADVDSNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLHH 240
           QFTNADVD NGLL+FDELKDFLHP+DSSNYRIQNWLL +KMK MD+DRDGKLNFDEFLHH
Sbjct: 181 QFTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHH 240

Query: 241 AYNTYRNYIEFETQGDNVPSAKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHYT 300
            YN Y+NYIEFETQGD++P+A+EKFDELDLD D++L+V+E+RPLFQY +PGE+S+AQHYT
Sbjct: 241 TYNIYKNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYT 300

Query: 301 SHLFNEADDNKDGNLTLDEMLNNENVFYSTIYDNHNGNYEDD-YRDEL 345
           SHL NEADDNKDG LT+DEMLN+E  FYST+Y+N NG+YEDD Y DEL
Sbjct: 301 SHLINEADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343

BLAST of Sed0001018 vs. TAIR 10
Match: AT4G27790.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 297.0 bits (759), Expect = 2.0e-80
Identity = 151/345 (43.77%), Postives = 222/345 (64.35%), Query Frame = 0

Query: 1   MERAVVY-VLTAAAFALFFVFPPSKSRDRNHHGLTTRRLGYKFRNPNFDPLVAEMERNAE 60
           M + VVY +LTA    L  +    +++ ++  GL TRR+G +   P FDPLV  +ER + 
Sbjct: 1   MAKVVVYTILTATIIFLILLAHKKQNQTQSIEGLITRRIGRRLEMPVFDPLVTRIERLSH 60

Query: 61  ESGLGSRESAIGVGKTAIPDDYRRYHDGE-KLNISLRLLVLFPLLDKSPKNGAISYEELG 120
           E   G++   +   K    D +  Y   E +LN ++R+  LFPLLD SP++G +S +EL 
Sbjct: 61  EKEAGTK--TVEAAKEEKDDMFEEYFAQERRLNTTMRIKFLFPLLDASPRDGFVSLKELQ 120

Query: 121 DWVTGQAIDRLNYRTRKQLEFSDKNGDGAISFHEYLPQFTEEDIARNGTEHGEAGWWKRQ 180
            W+  Q  D + YRT K+LE  DK+ DG I+F EYLPQF+++DI +N   HGEAGWW  Q
Sbjct: 121 TWMMQQTEDNMVYRTAKELELQDKDKDGVITFEEYLPQFSKQDIEKNEKGHGEAGWWMEQ 180

Query: 181 FTNADVDSNGLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLHHA 240
           F N+D D NG L+ +E  +FLHP+DS N   Q W+L+E+M  MD + DGKL + EF+ +A
Sbjct: 181 FKNSDFDHNGSLDIEEFNNFLHPEDSRNGDTQRWVLKERMTGMDTNGDGKLEYKEFVKNA 240

Query: 241 YNTYRNYIEFETQGD-NVPSAKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHYT 300
           Y  Y+ + +FE + D NVP+ +  F E+D D D+ L  DELRP+ QY  PGE+S+A+ Y+
Sbjct: 241 YEMYKEFAKFEKEEDENVPTPQLLFAEMDRDKDRFLVADELRPILQYLQPGEMSYAKFYS 300

Query: 301 SHLFNEADDNKDGNLTLDEMLNNENVFYSTIYDNHNGNYEDDYRD 343
           + L +EAD++KDG L+L+EML++E+VFY  ++  H    ++DY D
Sbjct: 301 TFLCHEADEDKDGKLSLEEMLHHEDVFYKAVH--HEDLDDEDYFD 341

BLAST of Sed0001018 vs. TAIR 10
Match: AT5G08580.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 191.0 bits (484), Expect = 1.5e-48
Identity = 127/389 (32.65%), Postives = 195/389 (50.13%), Query Frame = 0

Query: 4   AVVYVLTAAAFALFFV-FPPSKSRDR-NHHGLTTRRLGYKFRNP-----------NFDPL 63
           +V+  +T     LF V + P K  D  +HHG   +    K R+             FDPL
Sbjct: 5   SVILYITVGILVLFLVSYSPKKKGDHDHHHGGHNQHHRLKLRSSFNFKPTRHDPVPFDPL 64

Query: 64  VAEMERNAE--------------ESGLGSRESAIGVGKTAIP--------------DDYR 123
           VA+MER  E              E    S++   G G    P              +DY 
Sbjct: 65  VADMERRREDKEWERQYIEHSHPELVSHSQKETTGGGHEHAPGHESQPEWEEFMDAEDY- 124

Query: 124 RYHDGEKLNISLRLLVLFPLLDKSPKNGAISYEELGDWVTGQAIDRLNYRTRKQLEFSDK 183
             +D EK N++ RL++LFP +D SP +G ++  EL +W    +   + +RT++ L+  D+
Sbjct: 125 -LNDEEKFNVTDRLILLFPKIDVSPADGFMTESELTEWTMQSSAKEVVHRTQRDLDVHDR 184

Query: 184 NGDGAISFHEYLPQFTEEDIARNGTEHGEAGWWKRQFTNA-DVDSNGLLNFDELKDFLHP 243
           N DG ISF EY P         N   + + GWWK +  NA D + +GLLN  E  DFLHP
Sbjct: 185 NKDGFISFSEYEPPSWVRKSDNNSFGY-DMGWWKEEHFNASDANGDGLLNLTEFNDFLHP 244

Query: 244 QDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLHHAYNTYRNYIEFETQGDNVP----- 303
            D+ N ++  WL +E+++  D+D+DGK++F+EF H  ++T RNY E +      P     
Sbjct: 245 ADTKNPKLLLWLCKEEVRERDSDKDGKISFEEFFHGLFDTVRNY-EEDNHNSTHPYHDLP 304

Query: 304 --SAKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHYTSHLFNEADDNKDGNLTL 344
              AK+ F +LD + D  L+  EL P+    +P E  +A+    ++ ++AD +KD  LTL
Sbjct: 305 EGPAKQLFSQLDKNDDGYLSDVELLPIISKIHPTEHYYAKQQADYIISQADSDKDRRLTL 364

BLAST of Sed0001018 vs. TAIR 10
Match: AT2G41100.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 62.8 bits (151), Expect = 6.3e-10
Identity = 57/241 (23.65%), Postives = 109/241 (45.23%), Query Frame = 0

Query: 100 FPLLDKSPKNGAISYEELGDWVTGQAIDRLNYRTRKQLEFSDKNGDGAISFHEYL----- 159
           F L DK+  +G+I+ +ELG  +            +  +  +D +GDG I F E+L     
Sbjct: 17  FRLFDKN-GDGSITKKELGTMMRSIGEKPTKADLQDLMNEADLDGDGTIDFPEFLCVMAK 76

Query: 160 -------PQFTEEDIARNGTEHGEAGWWKRQFTNADVDSNGLLNFDELKDFLHPQDSSNY 219
                  P+ T++ +A   T+  +   ++  F   D + +G +   EL+  +     +  
Sbjct: 77  NQGHDQAPRHTKKTMADKLTD-DQITEYRESFRLFDKNGDGSITKKELRTVMFSLGKNRT 136

Query: 220 RIQNWLLREKMKSMDNDRDGKLNFDEFLH----------HAYNTYRNYIEFETQGDNVPS 279
           +     L++ M  +D D DG ++F EFL+             +T +  ++++   D +  
Sbjct: 137 KAD---LQDMMNEVDLDGDGTIDFPEFLYLMAKNQGHDQAPRHTKKTMVDYQLTDDQILE 196

Query: 280 AKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHYTSHLFNEADDNKDGNLTLDEM 319
            +E F   D +GD  +TV+ELR   +  + GE +  +     + NEAD + DG ++  E 
Sbjct: 197 FREAFRVFDKNGDGYITVNELRTTMR--SLGE-TQTKAELQDMINEADADGDGTISFSEF 249

BLAST of Sed0001018 vs. TAIR 10
Match: AT2G41100.4 (Calcium-binding EF hand family protein )

HSP 1 Score: 62.8 bits (151), Expect = 6.3e-10
Identity = 57/241 (23.65%), Postives = 109/241 (45.23%), Query Frame = 0

Query: 100 FPLLDKSPKNGAISYEELGDWVTGQAIDRLNYRTRKQLEFSDKNGDGAISFHEYL----- 159
           F L DK+  +G+I+ +ELG  +            +  +  +D +GDG I F E+L     
Sbjct: 17  FRLFDKN-GDGSITKKELGTMMRSIGEKPTKADLQDLMNEADLDGDGTIDFPEFLCVMAK 76

Query: 160 -------PQFTEEDIARNGTEHGEAGWWKRQFTNADVDSNGLLNFDELKDFLHPQDSSNY 219
                  P+ T++ +A   T+  +   ++  F   D + +G +   EL+  +     +  
Sbjct: 77  NQGHDQAPRHTKKTMADKLTD-DQITEYRESFRLFDKNGDGSITKKELRTVMFSLGKNRT 136

Query: 220 RIQNWLLREKMKSMDNDRDGKLNFDEFLH----------HAYNTYRNYIEFETQGDNVPS 279
           +     L++ M  +D D DG ++F EFL+             +T +  ++++   D +  
Sbjct: 137 KAD---LQDMMNEVDLDGDGTIDFPEFLYLMAKNQGHDQAPRHTKKTMVDYQLTDDQILE 196

Query: 280 AKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHYTSHLFNEADDNKDGNLTLDEM 319
            +E F   D +GD  +TV+ELR   +  + GE +  +     + NEAD + DG ++  E 
Sbjct: 197 FREAFRVFDKNGDGYITVNELRTTMR--SLGE-TQTKAELQDMINEADADGDGTISFSEF 249

BLAST of Sed0001018 vs. TAIR 10
Match: AT2G41100.3 (Calcium-binding EF hand family protein )

HSP 1 Score: 55.5 bits (132), Expect = 1.0e-07
Identity = 48/201 (23.88%), Postives = 92/201 (45.77%), Query Frame = 0

Query: 140 SDKNGDGAISFHEYL------------PQFTEEDIARNGTEHGEAGWWKRQFTNADVDSN 199
           +D +GDG I F E+L            P+ T++ +A   T+  +   ++  F   D + +
Sbjct: 21  ADLDGDGTIDFPEFLCVMAKNQGHDQAPRHTKKTMADKLTD-DQITEYRESFRLFDKNGD 80

Query: 200 GLLNFDELKDFLHPQDSSNYRIQNWLLREKMKSMDNDRDGKLNFDEFLH----------H 259
           G +   EL+  +     +  +     L++ M  +D D DG ++F EFL+           
Sbjct: 81  GSITKKELRTVMFSLGKNRTKAD---LQDMMNEVDLDGDGTIDFPEFLYLMAKNQGHDQA 140

Query: 260 AYNTYRNYIEFETQGDNVPSAKEKFDELDLDGDKLLTVDELRPLFQYFNPGELSHAQHYT 319
             +T +  ++++   D +   +E F   D +GD  +TV+ELR   +  + GE +  +   
Sbjct: 141 PRHTKKTMVDYQLTDDQILEFREAFRVFDKNGDGYITVNELRTTMR--SLGE-TQTKAEL 200

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141310.12.0e-14372.99reticulocalbin-2 [Momordica charantia][more]
XP_011651314.14.5e-14373.78reticulocalbin-2 [Cucumis sativus] >KGN57652.1 hypothetical protein Csa_010748 [... [more]
XP_023541445.12.2e-14273.49reticulocalbin-2 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022945265.13.8e-14273.20reticulocalbin-2 isoform X1 [Cucurbita moschata][more]
XP_022968155.16.5e-14273.78calumenin-B isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8BP922.7e-1326.37Reticulocalbin-2 OS=Mus musculus OX=10090 GN=Rcn2 PE=1 SV=1[more]
Q7ZUC23.5e-1324.2845 kDa calcium-binding protein OS=Danio rerio OX=7955 GN=sdf4 PE=2 SV=1[more]
B5X4E07.8e-1325.85Calumenin-B OS=Salmo salar OX=8030 GN=calub PE=2 SV=1[more]
Q3T0K11.3e-1222.09Calumenin OS=Bos taurus OX=9913 GN=CALU PE=2 SV=1[more]
Q627031.3e-1226.03Reticulocalbin-2 OS=Rattus norvegicus OX=10116 GN=Rcn2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CHQ29.8e-14472.99reticulocalbin-2 OS=Momordica charantia OX=3673 GN=LOC111011736 PE=4 SV=1[more]
A0A0A0LAH32.2e-14373.78Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G238180 PE=4 SV=1[more]
A0A6J1G0G21.9e-14273.20reticulocalbin-2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449565 PE=4 S... [more]
A0A6J1HWE33.2e-14273.78calumenin-B isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467475 PE=4 SV=1[more]
A0A5D3CJD29.2e-14272.99Reticulocalbin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001... [more]
Match NameE-valueIdentityDescription
AT4G27790.12.0e-8043.77Calcium-binding EF hand family protein [more]
AT5G08580.11.5e-4832.65Calcium-binding EF hand family protein [more]
AT2G41100.16.3e-1023.65Calcium-binding EF hand family protein [more]
AT2G41100.46.3e-1023.65Calcium-binding EF hand family protein [more]
AT2G41100.31.0e-0723.88Calcium-binding EF hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 95..124
e-value: 110.0
score: 1.3
coord: 174..202
e-value: 0.32
score: 20.1
coord: 296..328
e-value: 130.0
score: 0.6
coord: 132..160
e-value: 33.0
score: 5.5
coord: 213..241
e-value: 0.048
score: 22.8
coord: 257..285
e-value: 16.0
score: 8.1
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 219..234
e-value: 0.056
score: 13.0
coord: 141..152
e-value: 0.036
score: 13.6
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 258..318
e-value: 1.5E-9
score: 38.1
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 214..244
score: 9.548024
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 170..205
score: 9.63171
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 253..288
score: 10.078037
NoneNo IPR availableGENE3D1.10.238.10coord: 238..324
e-value: 1.6E-11
score: 46.3
NoneNo IPR availableGENE3D1.10.238.10coord: 156..237
e-value: 1.2E-11
score: 47.0
NoneNo IPR availableGENE3D1.10.238.10coord: 93..155
e-value: 2.3E-5
score: 26.4
NoneNo IPR availablePANTHERPTHR10827RETICULOCALBINcoord: 1..344
NoneNo IPR availablePANTHERPTHR10827:SF77CALCIUM-BINDING EF HAND FAMILY PROTEINcoord: 1..344
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 305..317
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 183..195
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 141..153
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 266..278
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 222..234
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 171..336
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 95..155

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0001018.1Sed0001018.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005509 calcium ion binding