Pay0021473 (gene) Melon (Payzawat) v1

Overview
NamePay0021473
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Descriptionbifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4
Locationchr09: 21304429 .. 21308813 (+)
RNA-Seq ExpressionPay0021473
SyntenyPay0021473
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAACCAGAAGAGGAAACCAAGAGCGGGAGCAAAACCCCAATCTTTATACACTGTGTTTGGTTTCGATACTATCACTCGAACATAATATATAAAAAAATGATACATATATTAAAATTTGAATAAAGAAAATGGATGAGTAAGAACTCATTTCAGCCCCAATATTTTGAGTCATTCCTCCAATTATCCGTTTACTGATTTATTTGTTTAAGGAAATTATCCCTTTTATATCAATCCTTCATTTTTCATCTTAATTAATTCGAATCAATAGCGATTCTGCCTCAAGAAGCTCCGCTGCAAATAGGGAAATTGGTGGGAGAAATTTGTGAAAAGCGAAAATGGCAGCAACAACAATTTCATCTTGTATTTTCTGCCAGAAAGCTTCTAATTCTACCGCCAATAACCTTCTTCATTTTGTCTGCTCCTTTCGTTTCTCTGATTAAAACCATTTTTCTTGTGCTTCTTCTTCTTCTTCTCTTATACCGGCGATTGAATGAAGCGTCCCTGATTAAGTATATCGGATTCTGCAGGATGAGAGAGTCGTTGCTTTTGAAGATATAAACCCTTCTGCTGTCAGGTATTTTGAACCTCTTAAAATATCGAATGAACCCTTTTTTCTAGCGTTTGATTTAGTTTTGCTATCGATATGAGGCTTCTGAGATATCTTCTGTGTTAATTTAATCTTCATTTCTGCATTGATTTAGCTTCTTTAGGGGAAATTCTTCTACTTTTGTATACCCATTTCGTCAGTTGCTTGATATTCAATGGACAATCTACATCTAGACCTGGATAGCAAATAGCAACGGCGGCAGGAAAAATTTAGAAAGAGGCAATGTTTTGGATCTTTGGGCAAAAGTAATGCTAAACGGATTTATTTTTGAGGGTCTTTTACATTTTAAGTTCTGAACTGCCCCAAAATATTTCCAGATATAAGGTTCACTATGTACATTCTAGGATGTCATTATCAATTGATGTCCTTGATAATCTGCTAAGCTCAAGAGCTTTTGTCGCTTATTTCAATCATAGTTTTTTATGATTCTTCCATTTGTTGGTTTTTAACATTTATGACCTGTTTGTAGTAGGTTCTCAGTGGCCATCTATGACCTTCGTGAGTTGCCATCTCGAAGGTGCTGAGGATGGCCTCTATAAGGCCACTACATGGTGAGTGTTAACTGTCATTGTGGATTCTGGAAAATCTAGAAAGAGATATATCCTTCACATACTAAGAAACAAAGAAATGACGGGTTCATTGTCAATCTCATTTTTCAATTGTTCGTGTTTCTTCAGCTCAAGTCATTTAGATTAATTGGTCAACTCATTTTCAATCCTTTAATTTGTATTAGGATGCACTAACAATTTCCACCACAAAAATGTTAGATGCAATTAATCTTCTGTAATTAAAGGCATCTATAAAACATTCTTACTTTGGGTTGCCATTTTGTAGGCATTTCTTGGTAATTCCCAAGGAGCATATTCCCACAGTTAGGAATCTCCAGAGAAGAGCTGAAGATTACTCACTCGGTAATGTAACCGTTGTTAAATGCAATTCATTTATGGTTTTTTGTTTAAAGAAACGGACAGGGGGAGGCAATGGGGTGGGGGAACAAGTCCTTAAAACGCAAGAGATATAGTTGCATGATATAACTTGATAAAACCTATTAGTACCTTTTTGTCTGCATTAAACCTTACCTTATTCTCTCCTTCCTTCGACCTCCCTTTTTGAACCCTCTCATTCTCAAAGCTAGCCTTGGGGTGGGAAAAACTGCAGGAAAATGAGTAGGCCATAATGCTCAACGTGGTCTTGACACTCCCGTTTTGAGAAAATAGTAAAGATTAGATATGCAAACAAAATTCTTCCTTTCTTTTTGAAGGAAAAAACCCAGGTGTCATATATGTGTCGTGGATCTAGACTAGAGTCCCACCTTCTAAAACACAAGGGTTTTCTTTTGTTTGGTAAGAAACTGAACTTTCTTTAAAAAAGAATGAGAGAATACAATGGATTACAAAAAACAAGCTTGAAAAACATGAGTTCAACACTAACTATGAAAAGAGACTCAAATTCAAAGGAATAAGGCTGGTATCATAACTACAAAACGGTCCAGTGACCAGCTTCCAACCAGAAGCATTAAAACTTGCTGACTCATAGACGTCCTCTCTAAATTTCTCCATATCCCTAAAAACTCCGCTATTAGGCTCCAACCCAAGTACCCACAAAATAGCGAAGAAACTCTCTTGTCACAACATTTTTCCCTTCTTCCTGGGGGAGCAAATGCAAAACCATCTTCTCCAACAAAGAACCCATCCCTGATACGATCCCCACTAACCCCAAAAGAACTCCAACAACGATCCCAAAGGCAGTTTGTGAAATGACACTCCACAGTAAATGACTTAGATCCTCCTACTGCCTCCCACAGAGATTGCATTTTTAAATCTTTGGTTTGCTTGCTAATTCTGATAATGACTTTGCTCATTTCTGTCTTGATTATTTTTAGCAGTAAGTCACATGTTGGGGGTGGGACAGACACTTTTATCCCAAGATTCACCTCAATTAAAGCACAGGTACACTGAATGATAACCACTCTTCAAAACCATTATGTTGCTAGTCTTCAGCAAGTAAACGGTGTTTATATTGTACTCTTCATTCAAATGATACAGGGTAAAACTAAAGAAAACATGGATTTCAATGAATTAACAATGTCTCACACTCTCAATCCTTTCATATGTTTGGTTGATTATATTGTACGTGGAGCTGGTTGTGCTTGCAACATATTATTGTGGAAACTTATAAAAATTGTTAATATTTGCATTACTTTATGATAATTCGTTGTGAACACTTTTATTAGTGTTTGAAAACCAATCTCCTAAAAAGCTATTTTAAGGACATAGTACCTGGTAGCATCTTAGTTCTTTCTTCCAAGGTGGACCAAGTGATGAATAATGTACTGTAATTGTGAATTCCTGCGAGTTTTGTTGTTCAAGTTCTACTACAAACTTTGTCTAGTGCGAAAGGATATCTTAGCTTCATTTTCTAGATGGCATGATGGAGAATCAGAGATAGATCGCCTTCTGTTCAGCAGAACGGATATAGGATCGATACCTGAAGAGAAGATTATCTTCTGGTCAATGGACTTTGGGCAAAGAAAAAGGAGTCTTGGTTCGGTTAATGATCAGGAGGTAGAAAGTGCGTTAACAAACTTGAATAAGAAAAAATTCACATCACATGTAAAGATAGTTTCCAGGCATTCCTTGGAATCTGATTCAGCATAACAGGTATTGAAAACCAGAGGTTTCTTTCCAAGGGGAAGGGGGGTGGATGACACTTACAAACTTTGGCTCCAATGAAATTCCAGAGTTGAATACATGAATTGTTCTGATTTGGATTTCTTTGAAAGGGATTGATATAGGAATGACATAGGGATCAAAATAGTCACTATTTAAAAGTACAAAAACCAAACGGATGTTTCAACTTGGACTAAAATCAACAAAAGTTGAAAATACAAAGACCAAAATCAACCGAAGCTGAAAGTCCATAAACCAAATTAGTATTTAAACCTTCTTTTTACTACTGACATGAAATACTAAATGCTTCACAGTTCTTCTGCTTTCAACTCTACTTTTAGTTCTTATCTTTTACTTTAGAACAGCATGATTCTAAAATTGATGCTTCTCTTCTCTTTCTCTGAAAGATTTGGCTTCCATCAGCCACCAATGAACAGTGTCAATCACCTACATCTCCATTGTTTTGCTCTTCCCTACGCACCTCGGTAATCAGTTTCTCAGTCTTGTTGATGTGAAATTTGATACTGTTTCTTTCAACAAAATACTGAACCGAAACTGCGCGCAAACAGGTGGAAGTTTGTAAAATATTTATCATTGGGATCGATCGGGTTCATTGAAGCTGAGAAGTTGTTGGAGAAAATAAAGCCTTAACTCACACACCAGATCACTTCAACAGTAGTGAGTATGCGTTCTCTATGATTGTTCACTGTTATAAAGAGCAGAAAAGTTTGATAACTCCGACCAAAATTAAGGCATCTATTATGAGTGTATTTCTTCGTAGATTCAAAGTTTTGTGTCAATCTCTACAGGAATTGTTCTTTTAGTATTCACTGGAAGAAAGGCTTTGTTTTTATTGCTTCTGATTCAGGAAAGGGACCAATGGCTAGCCTTAAATTATCAAGATTCTTTTTTATTATTATCATTTTTATTTTGTAGTAGAATGAGAAGTAAGAGATGAATGTGTAAATGAGTAATCAATGTACAATTGCTCATATTTTCTCCCTTGGAAGAATATTTAGTTTCCAGGAAGTGTGAGTTTGGTATAATTTTGGGGAATAATAATATATTAAGGGAAGTACTCTGCAGAATTAGTTTGGAAAATAAATGC

mRNA sequence

GAAACCAGAAGAGGAAACCAAGAGCGGGAGCAAAACCCCAATCTTTATACACTGTGTTTGGTTTCGATACTATCACTCGAACATAATATATAAAAAAATGATACATATATTAAAATTTGAATAAAGAAAATGGATGAGTAAGAACTCATTTCAGCCCCAATATTTTGAGTCATTCCTCCAATTATCCGTTTACTGATTTATTTGTTTAAGGAAATTATCCCTTTTATATCAATCCTTCATTTTTCATCTTAATTAATTCGAATCAATAGCGATTCTGCCTCAAGAAGCTCCGCTGCAAATAGGGAAATTGGTGGGAGAAATTTGTGAAAAGCGAAAATGGCAGCAACAACAATTTCATCTTGTATTTTCTGCCAGAAAGCTTCTAATTCTACCGCCAATAACCTTCTTCATTTTGATGAGAGAGTCGTTGCTTTTGAAGATATAAACCCTTCTGCTGTCAGGCATTTCTTGGTAATTCCCAAGGAGCATATTCCCACAGTTAGGAATCTCCAGAGAAGAGCTGAAGATTACTCACTCGTAAGTCACATGTTGGGGGTGGGACAGACACTTTTATCCCAAGATTCACCTCAATTAAAGCACAGATTTGGCTTCCATCAGCCACCAATGAACAGTGTCAATCACCTACATCTCCATTGTTTTGCTCTTCCCTACGCACCTCGGTGGAAGTTTGTAAAATATTTATCATTGGGATCGATCGGGTTCATTGAAGCTGAGAAGTTGTTGGAGAAAATAAAGCCTTAACTCACACACCAGATCACTTCAACAGTAGTGAGTATGCGTTCTCTATGATTGTTCACTGTTATAAAGAGCAGAAAAGTTTGATAACTCCGACCAAAATTAAGGCATCTATTATGAGTGTATTTCTTCGTAGATTCAAAGTTTTGTGTCAATCTCTACAGGAATTGTTCTTTTAGTATTCACTGGAAGAAAGGCTTTGTTTTTATTGCTTCTGATTCAGGAAAGGGACCAATGGCTAGCCTTAAATTATCAAGATTCTTTTTTATTATTATCATTTTTATTTTGTAGTAGAATGAGAAGTAAGAGATGAATGTGTAAATGAGTAATCAATGTACAATTGCTCATATTTTCTCCCTTGGAAGAATATTTAGTTTCCAGGAAGTGTGAGTTTGGTATAATTTTGGGGAATAATAATATATTAAGGGAAGTACTCTGCAGAATTAGTTTGGAAAATAAATGC

Coding sequence (CDS)

ATGGCAGCAACAACAATTTCATCTTGTATTTTCTGCCAGAAAGCTTCTAATTCTACCGCCAATAACCTTCTTCATTTTGATGAGAGAGTCGTTGCTTTTGAAGATATAAACCCTTCTGCTGTCAGGCATTTCTTGGTAATTCCCAAGGAGCATATTCCCACAGTTAGGAATCTCCAGAGAAGAGCTGAAGATTACTCACTCGTAAGTCACATGTTGGGGGTGGGACAGACACTTTTATCCCAAGATTCACCTCAATTAAAGCACAGATTTGGCTTCCATCAGCCACCAATGAACAGTGTCAATCACCTACATCTCCATTGTTTTGCTCTTCCCTACGCACCTCGGTGGAAGTTTGTAAAATATTTATCATTGGGATCGATCGGGTTCATTGAAGCTGAGAAGTTGTTGGAGAAAATAAAGCCTTAA

Protein sequence

MAATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRAEDYSLVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVKYLSLGSIGFIEAEKLLEKIKP
Homology
BLAST of Pay0021473 vs. ExPASy Swiss-Prot
Match: Q84VV6 (Bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 OS=Arabidopsis thaliana OX=3702 GN=HINT4 PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 3.5e-52
Identity = 96/135 (71.11%), Postives = 110/135 (81.48%), Query Frame = 0

Query: 8   SCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRAEDYSL 67
           +CIFC+   N T   LLH DE+V+AF+DI P+A RH+LVIPKEHIPTV +LQRR EDYSL
Sbjct: 7   ACIFCEIVRNPTTTRLLHTDEKVIAFQDIKPAAQRHYLVIPKEHIPTVNDLQRRDEDYSL 66

Query: 68  VSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVKYLSLGSI 127
           V HML VGQ LL +D+PQ  HRFGFHQPP NSV+HLHLHCFALPY PRWK +KY SLG +
Sbjct: 67  VRHMLSVGQQLLQKDAPQSIHRFGFHQPPFNSVDHLHLHCFALPYVPRWKAIKYKSLGPL 126

Query: 128 -GFIEAEKLLEKIKP 142
            GFIEAE LLEKI+P
Sbjct: 127 GGFIEAETLLEKIRP 141

BLAST of Pay0021473 vs. ExPASy Swiss-Prot
Match: Q9NQE9 (Histidine triad nucleotide-binding protein 3 OS=Homo sapiens OX=9606 GN=HINT3 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.8e-16
Identity = 51/138 (36.96%), Postives = 78/138 (56.52%), Query Frame = 0

Query: 7   SSCIFCQKAS-NSTANNLLHFD-ERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRAED 66
           S+C+FC+ A        LLH + E ++ F+DI P+A  H+LV+PK+HI   R L  R + 
Sbjct: 46  STCVFCRIAGRQDPGTELLHCENEDLICFKDIKPAATHHYLVVPKKHIGNCRTL--RKDQ 105

Query: 67  YSLVSHMLGVGQTLLSQD--SPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVKYL 126
             LV +M+ VG+T+L ++  +     R GFH PP  S++HLHLH  A      +      
Sbjct: 106 VELVENMVTVGKTILERNNFTDFTNVRMGFHMPPFCSISHLHLHVLAPVDQLGFLSKLVY 165

Query: 127 SLGSIGFIEAEKLLEKIK 141
            + S  FI A+ L+EK++
Sbjct: 166 RVNSYWFITADHLIEKLR 181

BLAST of Pay0021473 vs. ExPASy Swiss-Prot
Match: Q5R9L4 (Histidine triad nucleotide-binding protein 3 OS=Pongo abelii OX=9601 GN=HINT3 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.8e-16
Identity = 51/138 (36.96%), Postives = 78/138 (56.52%), Query Frame = 0

Query: 7   SSCIFCQKAS-NSTANNLLHFD-ERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRAED 66
           S+C+FC+ A        LLH + E ++ F+DI P+A  H+LV+PK+HI   R L  R + 
Sbjct: 46  STCVFCRIAGRQDPGTELLHCENEDLICFKDIKPAATHHYLVVPKKHIGNCRTL--RKDQ 105

Query: 67  YSLVSHMLGVGQTLLSQD--SPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVKYL 126
             LV +M+ VG+T+L ++  +     R GFH PP  S++HLHLH  A      +      
Sbjct: 106 VELVENMVTVGKTILERNNFTDFTNVRMGFHMPPFCSISHLHLHVLAPVDQLGFLSKLVY 165

Query: 127 SLGSIGFIEAEKLLEKIK 141
            + S  FI A+ L+EK++
Sbjct: 166 RVNSYWFITADHLIEKLR 181

BLAST of Pay0021473 vs. ExPASy Swiss-Prot
Match: Q28BZ2 (Histidine triad nucleotide-binding protein 3 OS=Xenopus tropicalis OX=8364 GN=hint3 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 9.0e-16
Identity = 47/136 (34.56%), Postives = 80/136 (58.82%), Query Frame = 0

Query: 8   SCIFCQKASNSTAN-NLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRAEDYS 67
           SCIFC+ A+   +   LLH D+ +V F+DI P+   H+LV+PK+H+ T + L +  +   
Sbjct: 18  SCIFCRIANKQESGAELLHSDDDLVCFKDIRPAVTHHYLVVPKKHVGTCKTLTK--DHVQ 77

Query: 68  LVSHMLGVGQTLLSQDS-PQLKH-RFGFHQPPMNSVNHLHLHCFALPYAPRWKFVKYLSL 127
           L+  M+ VG++ L +++   L+  R GFH PP  S++HLHLH  A      +       +
Sbjct: 78  LIKTMMEVGKSTLQKNNVTDLEDIRLGFHYPPFCSISHLHLHVLAPASQLGFLSRMIYRV 137

Query: 128 GSIGFIEAEKLLEKIK 141
            S  FI A++L+++++
Sbjct: 138 NSYWFITADELIDQLQ 151

BLAST of Pay0021473 vs. ExPASy Swiss-Prot
Match: Q8K3P7 (Histidine triad nucleotide-binding protein 3 OS=Rattus norvegicus OX=10116 GN=Hint3 PE=2 SV=2)

HSP 1 Score: 80.5 bits (197), Expect = 1.7e-14
Identity = 50/140 (35.71%), Postives = 81/140 (57.86%), Query Frame = 0

Query: 7   SSCIFCQKASNSTANNLLHFDER--VVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRAED 66
           S+C+FC+ A+       L + E   +V F+DI P+A+ H+LV+PK+HI + ++L +  + 
Sbjct: 39  SNCVFCRVAAGQEPETELLYCENKDLVCFKDIKPAALHHYLVVPKKHIGSCKDLNK--DH 98

Query: 67  YSLVSHMLGVGQTLLSQD--SPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVKYL 126
             +V  M+ VG+T+L ++  +     R GFH PP  SV+HLHLH  A   A  + F+  +
Sbjct: 99  IEMVESMVTVGKTILERNNFTDFTDVRMGFHVPPFCSVSHLHLHVIA--PAKEFGFLSRV 158

Query: 127 --SLGSIGFIEAEKLLEKIK 141
                S  FI  + LLEK++
Sbjct: 159 VYRRDSYWFITGDYLLEKLR 174

BLAST of Pay0021473 vs. ExPASy TrEMBL
Match: A0A1S3AT56 (histidine triad nucleotide-binding protein 3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482613 PE=4 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 3.0e-75
Identity = 141/141 (100.00%), Postives = 141/141 (100.00%), Query Frame = 0

Query: 1   MAATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQR 60
           MAATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQR
Sbjct: 1   MAATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQR 60

Query: 61  RAEDYSLVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVK 120
           RAEDYSLVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVK
Sbjct: 61  RAEDYSLVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVK 120

Query: 121 YLSLGSIGFIEAEKLLEKIKP 142
           YLSLGSIGFIEAEKLLEKIKP
Sbjct: 121 YLSLGSIGFIEAEKLLEKIKP 141

BLAST of Pay0021473 vs. ExPASy TrEMBL
Match: A0A1S3ATA4 (histidine triad nucleotide-binding protein 3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482613 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 7.5e-74
Identity = 141/142 (99.30%), Postives = 141/142 (99.30%), Query Frame = 0

Query: 1   MAATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQR 60
           MAATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQR
Sbjct: 1   MAATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQR 60

Query: 61  RAEDYSL-VSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFV 120
           RAEDYSL VSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFV
Sbjct: 61  RAEDYSLAVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFV 120

Query: 121 KYLSLGSIGFIEAEKLLEKIKP 142
           KYLSLGSIGFIEAEKLLEKIKP
Sbjct: 121 KYLSLGSIGFIEAEKLLEKIKP 142

BLAST of Pay0021473 vs. ExPASy TrEMBL
Match: A0A0A0KKN0 (HIT domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G161990 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 2.3e-70
Identity = 133/140 (95.00%), Postives = 134/140 (95.71%), Query Frame = 0

Query: 2   AATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRR 61
           AA  ISSCIFCQKA NSTAN+LLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRR
Sbjct: 3   AAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRR 62

Query: 62  AEDYSLVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVKY 121
           AEDYSLVSHML VGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPY PRWKF KY
Sbjct: 63  AEDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFAKY 122

Query: 122 LSLGSIGFIEAEKLLEKIKP 142
           LSLGSIGFIEAEKLLEKIKP
Sbjct: 123 LSLGSIGFIEAEKLLEKIKP 142

BLAST of Pay0021473 vs. ExPASy TrEMBL
Match: A0A6J1E2J5 (bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 OS=Cucurbita moschata OX=3662 GN=LOC111430257 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 2.9e-70
Identity = 130/139 (93.53%), Postives = 134/139 (96.40%), Query Frame = 0

Query: 3   ATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRA 62
           A T SSCIFCQ+ASNSTANNLLHFDERVVAFED+NPSAVRH+LVIPKEHIPTVRNLQRRA
Sbjct: 2   AATASSCIFCQRASNSTANNLLHFDERVVAFEDVNPSAVRHYLVIPKEHIPTVRNLQRRA 61

Query: 63  EDYSLVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVKYL 122
           EDYSLVSHML VGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPY PRWKF KY+
Sbjct: 62  EDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFAKYI 121

Query: 123 SLGSIGFIEAEKLLEKIKP 142
           SLGSIGFIEAEKLLEKIKP
Sbjct: 122 SLGSIGFIEAEKLLEKIKP 140

BLAST of Pay0021473 vs. ExPASy TrEMBL
Match: A0A6J1I1U2 (bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 OS=Cucurbita maxima OX=3661 GN=LOC111468838 PE=4 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 6.6e-70
Identity = 129/139 (92.81%), Postives = 134/139 (96.40%), Query Frame = 0

Query: 3   ATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRA 62
           A T SSCIFCQ+ASNSTANNLLHFDERVVAFED+NPSAVRH+LVIPK+HIPTVRNLQRRA
Sbjct: 2   AATASSCIFCQRASNSTANNLLHFDERVVAFEDVNPSAVRHYLVIPKDHIPTVRNLQRRA 61

Query: 63  EDYSLVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVKYL 122
           EDYSLVSHML VGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPY PRWKF KY+
Sbjct: 62  EDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFAKYI 121

Query: 123 SLGSIGFIEAEKLLEKIKP 142
           SLGSIGFIEAEKLLEKIKP
Sbjct: 122 SLGSIGFIEAEKLLEKIKP 140

BLAST of Pay0021473 vs. NCBI nr
Match: XP_008437084.1 (PREDICTED: histidine triad nucleotide-binding protein 3 isoform X2 [Cucumis melo])

HSP 1 Score: 290.8 bits (743), Expect = 6.3e-75
Identity = 141/141 (100.00%), Postives = 141/141 (100.00%), Query Frame = 0

Query: 1   MAATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQR 60
           MAATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQR
Sbjct: 1   MAATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQR 60

Query: 61  RAEDYSLVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVK 120
           RAEDYSLVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVK
Sbjct: 61  RAEDYSLVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVK 120

Query: 121 YLSLGSIGFIEAEKLLEKIKP 142
           YLSLGSIGFIEAEKLLEKIKP
Sbjct: 121 YLSLGSIGFIEAEKLLEKIKP 141

BLAST of Pay0021473 vs. NCBI nr
Match: XP_008437083.1 (PREDICTED: histidine triad nucleotide-binding protein 3 isoform X1 [Cucumis melo])

HSP 1 Score: 286.2 bits (731), Expect = 1.5e-73
Identity = 141/142 (99.30%), Postives = 141/142 (99.30%), Query Frame = 0

Query: 1   MAATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQR 60
           MAATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQR
Sbjct: 1   MAATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQR 60

Query: 61  RAEDYSL-VSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFV 120
           RAEDYSL VSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFV
Sbjct: 61  RAEDYSLAVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFV 120

Query: 121 KYLSLGSIGFIEAEKLLEKIKP 142
           KYLSLGSIGFIEAEKLLEKIKP
Sbjct: 121 KYLSLGSIGFIEAEKLLEKIKP 142

BLAST of Pay0021473 vs. NCBI nr
Match: XP_038875694.1 (bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 isoform X1 [Benincasa hispida] >XP_038875695.1 bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 isoform X1 [Benincasa hispida])

HSP 1 Score: 276.9 bits (707), Expect = 9.4e-71
Identity = 133/139 (95.68%), Postives = 135/139 (97.12%), Query Frame = 0

Query: 3   ATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRA 62
           A T+SSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRH LVIPKEHIPTVRNLQRRA
Sbjct: 2   AATVSSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHLLVIPKEHIPTVRNLQRRA 61

Query: 63  EDYSLVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVKYL 122
           EDYSLVSHML VGQTLLSQDSP+LKHRFGFHQPPMNSVNHLHLHCFALPY PRWKFVKYL
Sbjct: 62  EDYSLVSHMLEVGQTLLSQDSPRLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFVKYL 121

Query: 123 SLGSIGFIEAEKLLEKIKP 142
           SLGSIGFIEAEKLLEKIKP
Sbjct: 122 SLGSIGFIEAEKLLEKIKP 140

BLAST of Pay0021473 vs. NCBI nr
Match: XP_004152404.1 (bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 isoform X2 [Cucumis sativus])

HSP 1 Score: 274.6 bits (701), Expect = 4.7e-70
Identity = 133/140 (95.00%), Postives = 134/140 (95.71%), Query Frame = 0

Query: 2   AATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRR 61
           AA  ISSCIFCQKA NSTAN+LLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRR
Sbjct: 3   AAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRR 62

Query: 62  AEDYSLVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVKY 121
           AEDYSLVSHML VGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPY PRWKF KY
Sbjct: 63  AEDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFAKY 122

Query: 122 LSLGSIGFIEAEKLLEKIKP 142
           LSLGSIGFIEAEKLLEKIKP
Sbjct: 123 LSLGSIGFIEAEKLLEKIKP 142

BLAST of Pay0021473 vs. NCBI nr
Match: XP_022922197.1 (bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 [Cucurbita moschata] >XP_023551645.1 bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 [Cucurbita pepo subsp. pepo] >KAG6579414.1 Bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 274.2 bits (700), Expect = 6.1e-70
Identity = 130/139 (93.53%), Postives = 134/139 (96.40%), Query Frame = 0

Query: 3   ATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRA 62
           A T SSCIFCQ+ASNSTANNLLHFDERVVAFED+NPSAVRH+LVIPKEHIPTVRNLQRRA
Sbjct: 2   AATASSCIFCQRASNSTANNLLHFDERVVAFEDVNPSAVRHYLVIPKEHIPTVRNLQRRA 61

Query: 63  EDYSLVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVKYL 122
           EDYSLVSHML VGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPY PRWKF KY+
Sbjct: 62  EDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFAKYI 121

Query: 123 SLGSIGFIEAEKLLEKIKP 142
           SLGSIGFIEAEKLLEKIKP
Sbjct: 122 SLGSIGFIEAEKLLEKIKP 140

BLAST of Pay0021473 vs. TAIR 10
Match: AT4G16566.1 (histidine triad nucleotide-binding 4 )

HSP 1 Score: 205.7 bits (522), Expect = 2.5e-53
Identity = 96/135 (71.11%), Postives = 110/135 (81.48%), Query Frame = 0

Query: 8   SCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRAEDYSL 67
           +CIFC+   N T   LLH DE+V+AF+DI P+A RH+LVIPKEHIPTV +LQRR EDYSL
Sbjct: 7   ACIFCEIVRNPTTTRLLHTDEKVIAFQDIKPAAQRHYLVIPKEHIPTVNDLQRRDEDYSL 66

Query: 68  VSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVKYLSLGSI 127
           V HML VGQ LL +D+PQ  HRFGFHQPP NSV+HLHLHCFALPY PRWK +KY SLG +
Sbjct: 67  VRHMLSVGQQLLQKDAPQSIHRFGFHQPPFNSVDHLHLHCFALPYVPRWKAIKYKSLGPL 126

Query: 128 -GFIEAEKLLEKIKP 142
            GFIEAE LLEKI+P
Sbjct: 127 GGFIEAETLLEKIRP 141

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84VV63.5e-5271.11Bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 O... [more]
Q9NQE91.8e-1636.96Histidine triad nucleotide-binding protein 3 OS=Homo sapiens OX=9606 GN=HINT3 PE... [more]
Q5R9L41.8e-1636.96Histidine triad nucleotide-binding protein 3 OS=Pongo abelii OX=9601 GN=HINT3 PE... [more]
Q28BZ29.0e-1634.56Histidine triad nucleotide-binding protein 3 OS=Xenopus tropicalis OX=8364 GN=hi... [more]
Q8K3P71.7e-1435.71Histidine triad nucleotide-binding protein 3 OS=Rattus norvegicus OX=10116 GN=Hi... [more]
Match NameE-valueIdentityDescription
A0A1S3AT563.0e-75100.00histidine triad nucleotide-binding protein 3 isoform X2 OS=Cucumis melo OX=3656 ... [more]
A0A1S3ATA47.5e-7499.30histidine triad nucleotide-binding protein 3 isoform X1 OS=Cucumis melo OX=3656 ... [more]
A0A0A0KKN02.3e-7095.00HIT domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G161990 PE=4 SV... [more]
A0A6J1E2J52.9e-7093.53bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 O... [more]
A0A6J1I1U26.6e-7092.81bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 O... [more]
Match NameE-valueIdentityDescription
XP_008437084.16.3e-75100.00PREDICTED: histidine triad nucleotide-binding protein 3 isoform X2 [Cucumis melo... [more]
XP_008437083.11.5e-7399.30PREDICTED: histidine triad nucleotide-binding protein 3 isoform X1 [Cucumis melo... [more]
XP_038875694.19.4e-7195.68bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 i... [more]
XP_004152404.14.7e-7095.00bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 i... [more]
XP_022922197.16.1e-7093.53bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 [... [more]
Match NameE-valueIdentityDescription
AT4G16566.12.5e-5371.11histidine triad nucleotide-binding 4 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001310Histidine triad (HIT) proteinPRINTSPR00332HISTRIADcoord: 31..49
score: 59.37
coord: 98..108
score: 48.0
IPR036265HIT-like superfamilyGENE3D3.30.428.10coord: 10..141
e-value: 3.0E-27
score: 97.7
IPR036265HIT-like superfamilySUPERFAMILY54197HIT-likecoord: 6..129
NoneNo IPR availablePFAMPF11969DcpS_Ccoord: 8..115
e-value: 4.6E-25
score: 88.3
NoneNo IPR availablePANTHERPTHR12486APRATAXIN-RELATEDcoord: 4..140
NoneNo IPR availablePANTHERPTHR12486:SF5HISTIDINE TRIAD NUCLEOTIDE-BINDING PROTEIN 3coord: 4..140
IPR011146HIT-like domainPROSITEPS51084HIT_2coord: 10..118
score: 12.057672

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0021473.1Pay0021473.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0047627 adenylylsulfatase activity
molecular_function GO:0003824 catalytic activity