Pay0021410 (gene) Melon (Payzawat) v1

Overview
NamePay0021410
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Descriptiontaxadiene 5-alpha hydroxylase
Locationchr05: 6008185 .. 6013537 (-)
RNA-Seq ExpressionPay0021410
SyntenyPay0021410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCTCAAAGTCTTTCACAAAGAGGTACAAAATTTTGAGTTTTAACTTTTCATATGCATGAATATAGTATTTCAATTCTGAAATTTAAAGTGAAGGGAGGCAAATTAGAGAAAGGTAACTTTCAATACTCTATATCTTAGTTAGATTTTTTTTTTAAGAATTATCAAACTTACTTTAATTGGTCTATTTTGTTAAATCGACAATTAAAAAATAATTCATGGACGAATGATTTTTAAAGATAATTGTAATGTTAGATTTATGAAAATTTAGGAGTTTAATTAATTCAATTTACAAATTTAACATGATATTAGTCGAATAAAATCTAAAGGAGGGTACACTTGCATAAGTTCAAATTTTAAACGGACCACTTATTAGATTAAGCTTTTAAATGATACAATCACAAAAGTTTAAAGTATAAATTAATATTTTCCCAACATATCTTTACTTGAAATAAGCTCAATAGGATGGATAACGAGGTGTGACTACCTTCACAATCTTCTCCTTTTTGTCCCTAAATCGGCCAGTGCCCTCTTCCTATTTAGCACTAAGAAACTATTTGAGAATGTTGCTACGATGGGATAGAACAATTTTATATCTTTGTATGAAAGAGGATCTTTGAAGGTAATTAAAATTTATATTCCTTAGCAGATATGTAAATTTTGTAGATATGGCTAAACCGTGGAAATTGATCAGTAATAACTTTTTCTTTGCTGGACATTTGATTCACCCACCTACCTAAGCTAGTGGTGATTTGTTAAAATATAGGCATGCGACTTCACATTTAACACTTCTAAAAATCTGAAAGGTATTGTATATGAGTTAATGAATGTTATTGAACTTAGATGTGAAGATTTCTCGATTAAACAAGAAATGTGAGGAGTGGGGAGAATTTTCTTCCTGTTGGTTAAACGGAGATGGAAACGAGAATTATATTCCCAGACCTTGACTTGCTTTATTTATTTATTTTTTGATTCTGTACATATTATAGATGAAAACTTCATATCATTCCTCATCTTCTTGTGTGTGTATGAATATACGTACTTCATATATATTCCTCTGGTGTGTGTTTGTTAAGGATAAAAAGAAAGCAAACCAAGTCAGCAAGACCAACATTCTCAGCATATCCACTTCAGGTAAAATCAATAATATATTAGGAATAAAATCTTGAAAATTTGTCCAAAAGCTGTACTTTTATCAGCCCGCATACCAATGGAGATGGATTTTGATCTGCATGGGCCTATCATGTTTGATAATACGAGGTTCTATTTCAAAACTAATTGATAATGAAATGAATAGTCCATCTACTCATAAGGAATGTGAGTCCCCTTAATTTTTCTAATTTTTCTAATGTGAGATTATTAATATCTCAACAAAGGTTGCTATAAGATAAGAGTTCCTTGATAGAAAACTATTTTCATGTTTTTGGACCAATAAAAAATTGAAGTTAATATTACACCATCCAAATATATCCCTTTTCGAATAATATGATATATACGTCTATTTCTCGTGGAATAGAAAATATAGTATATAGTTATGCAGTAATTGCCATGTATTTCTCTTTTCTAAAGATTAGTTTTAATTTCCTTTTGTCCAACAAATTCTCAACAAATTCTTCTATAGTCAAAAATTTTAAAGATTAGCTGGGCTAACTGCAAGATGCAAAGTAGAATAATTGTGTAATATGTTAATTTAAGAAATTGTTGAATAGGTTGAGTTATAGAGATATGTTGGACTCATATTAAAATTTGGCATTTTTTTTTGGTATCTATTTTGTTATTTAAAACTAAACCTAGCGGTAAATATTACATTTACATTCATCTTTCTTCTTGTGTTACCTATATCTTACTAATAATTTAAAAAACCAAATCAAAATTTGAAAGCTAGAAAAAATATAAAAGTTATTTTTGTTTTTTGATTTTGTCTCACAATTTAACTACTAGAAAGATGCAAACCATTATAAGAAAATGGAAAGAGATAGACTTAATTTTCAAAAACCAAAAAACAAACAAAAAAAATAAAATAATTATGAGGATTTGCACTAAAATCAATATATGGTTGTTTGTGTAACATACTTTAGCTAGATCTTCTTTATATTATACTTGTACCATGCAAATATATACATAAAATATAGACATGTTGAAGATATAAGCACCCATTGTATATGCCTATATGGTGTGTAAAGTTCATTTAATTAAAACAAAACTTGGTATAAGGGTCTTCTCTAGCTATTTCAGTATATATCATCCTCATTCAGAATCATAATACTTCAACCATTTAAGTTGAAATTTGTATGTACGATTCCATAATTCTTAAAGTAACTCATTTATTCACTTCAAACATGACTTTTTATCTTACTTATTATTATTTTGGGTTTAACAAGTGAAGGACCGAAGGTATGTCTTGGAAAGTGTCCTCTCCAAATCAAAGAATATATATTAACTTTTTTTCATTTTGCAATCGGGAGTTTTCTTTAGAATGTTGTATGTTGTGCAGAAAACTCACATATATACTACTTTATGCTCTCGTTTCGTTCCACTACTTTGATACATATACGTTAGTTAGACCTAGTCTACACAAGAGTATATATATTGATGTTGTATCGAGTCGATTAGACTCTGATGCCTAAGTTAGGACACAAAATATTTAGCTCAGCCAAAGTATAGAGAGAATTGCTTAACCTTTAAGATGGTTAGTGTGAATAAGGTCCATCCTCTAAAGTTCTAATTTCTGTTTGGAGATGTTCAATGATTGATGATGTCAAAACGAACTTAACTCAATCGTTTTCTTCTTAGTGAAATTCCACATGCTCCGGCCAATATTTGAGGATTGGACCACCCTTAGGCTTGAGTCTTGTTTCAAGCAACTCTCGACATAGCAGTAGAATAATTAAGGAGAAAATTTGAACAATTGATTATGGTTTCGAGTTTGAATTGTATATCTCATATTTACGTGTTCTTTATTCGAGGGGTACATATTTTTCTAAAGAAAAAAAACGTAGGTAAGTTATTATTTGCAGTCTATTTTATTAGTTCAATCATAAATTAATAATAAGAAACCGTTTTTTTCTTTTCTTTTTATTAAGATTAACATAGATACAATTCAAGATGCACTCAAGTATCGTTTTTTCTATTGATTTATAAGATACATATTATATTATTCTATATATGCACATGCAAACCCCACGATTTTAGCTTTACCATAGCTTAACTGATTATGAAAGTCATGTTTGTTCGTCAACTTCAATCAAAAGATTTTGACCATTTATTAAAATACAAAACAAAACAGCAATGCTGATTAGTTGACTTCCTACACCAATCATATCATCTTCTCTTAAAATGCGATTTCCATCTCTTCCCAACACATAAATTTCTCTCACAAGGAATTTATTTGCTTTCTATTCACAATAACAATGCTCTCTTTTTTAGCTGCAATCCTGTTAATAATAACGATTGCTTTTGTGTTGAGAAGCAATAAGAGAAGCAAAAATGTCCCACCAGGGGAAATGGGTCTACCTTGGATTGGAGAAACAATGGCTTTTTACAGAGCTCAAAGTAAAAACAGATTGTTTGAAGACTTTGTTGAGCCAAGAGTAGCAAAATATGGAAAAATCTTCAAAACAAACTTAATGGGATCTCCAACAGTGGTTGTTAATGGAGCTGAAGCCAATAGATTTTTCTTGTCAAATGAGTTTAAGTTAGTGGTAAGTTCATGGCCATCGAGCTCCGTTCAGTTGATGGGTGAAGAATGTATTATGCAGAAGGAAGGTGAAAAGCATAGATGTGTTCGTGGTCTTATTGCCTCAGCTCTTTCTACTTCGAGTCTTGAAGTTATGATGCCTAAACTGTGCAATACAATTCAGGTTTATTTGGATACTAAGTGGGTTGATTGTGACACAGTTGGTCTCTTTCGCTCGGCTAAAGTATTGACATTTACCATTGTGTTTGAGTGTTTATTGGGTATTATAGTGGAGCCACGTGTGTTAACGACGTTTGAGAGGGTTTTGGAAGGTGTTTTTGCACCACCATTCAGGTAGTTCTTTAAAGTTTTAAGCTGATTGGTTATGTTCAAAGTTAATTGCATTTTCATCTATTTTTTGCACCACCAAGTTTTTATAAGTATTGTTTTCAACCATCAAATAGTTTTCAAACGGTACCTTTAACTTTTAAAACTTTGATTGATTTGTAAACATGTTGGGAGAACTTGAATAACATAGGTAAAAATAGTTATCATAAGTTTAACTTTTAAAAAATAATAATAAAAAAAAGAACTAAAACAAAATTGGTATCAAATAACATGTTCATGTTTTTAACACAGGCTTCCAGGCACAAGATTTTCAAGAGCCAAGAAAGCAAGACTGGAGATAGAGACAATGCTAAGGGAAGTGGTGAGAGAGAAGAGGAGAAAAATTGAAGGAAAACTAGAAGAAGAAGAACAATGTGGGTCATTGCTTTCAAGGATGGTGAGAGCAATGATAGGAGGGGAAATCAGTGAAATGGAAGTCGTAGACAATATTGTTTTGCTTGTTTTTGCAGCTCATGATACGACCTCCTTTGCCATTGCCATGACTTTCAAAATGTTGTCTCAGCACACAAACTGCTACTCACTTCTTCTCCAGGGTAATCTTTCCTTTCTTCAATCTTCTGAAGTATACTCTAAGATCATAATAAACCAAATATGGCTATTCTGATGGTTGTCAAAATATCATTTTACTGATTTAAATGGGTTCTAAAATGTTTTTACTTTTAAACAGAACACGCTGACATAGTAAGAAGCAAGGGGCCAGGCCAGAATTTGACAATGGAAGACATAAAGAAGATGAAATATACTTGGCAGGTAGCTCGGGAGAGCATTAGATTGTTTCCCCCTATATTTGGTTCTTTCAGGAAAGCCATTTCAGACATTGAATACGAGAGCTTTCTCATTCCCAAAGGATGGAAGGTGAAAATTTCAATCCTTACAGCGATACCCCTGCAGTCTAATCTGGTCCAGAAACGACCATCATTTTAGCTCGAATCATTTTGGTTTCATGGCAGGTCTTATGGACAACGTACGGAACACATTACGGAGAAGAGTACTTTGGAGATCCATTGAGTTTCAAGCCAAGCAGATTTGAGGAACCTGTTTCTCAGTATGTTTATGTTCCATTTGGAGGAGGGCCAAGAGCTTGTGCAGGCTACCAGCTAGCGAAATTGAACATACTCATCTTTATACACTCGGTTGTAACCCGTTATGATTGGAGTTTAGTTCATCCAGATGAGCCAATCATAATGGATCCTCTCCCTGTCCCTTATCATGGAATGCCTATTAAGATTTCTCCAAAATCAGAAATTTGTCCCAATGTTCACCAAATGTAA

mRNA sequence

AGCTCAAAGTCTTTCACAAAGAGGATAAAAAGAAAGCAAACCAAGTCAGCAAGACCAACATTCTCAGCATATCCACTTCAGCAATAAGAGAAGCAAAAATGTCCCACCAGGGGAAATGGGTCTACCTTGGATTGGAGAAACAATGGCTTTTTACAGAGCTCAAAGTAAAAACAGATTGTTTGAAGACTTTGTTGAGCCAAGAGTAGCAAAATATGGAAAAATCTTCAAAACAAACTTAATGGGATCTCCAACAGTGGTTGTTAATGGAGCTGAAGCCAATAGATTTTTCTTGTCAAATGAGTTTAAGTTAGTGGTAAGTTCATGGCCATCGAGCTCCGTTCAGTTGATGGGTGAAGAATGTATTATGCAGAAGGAAGGTGAAAAGCATAGATGTGTTCGTGGTCTTATTGCCTCAGCTCTTTCTACTTCGAGTCTTGAAGTTATGATGCCTAAACTGTGCAATACAATTCAGGTTTATTTGGATACTAAGTGGGTTGATTGTGACACAGTTGGTCTCTTTCGCTCGGCTAAAGTATTGACATTTACCATTGTGTTTGAGTGTTTATTGGGTATTATAGTGGAGCCACGTGTGTTAACGACGTTTGAGAGGGTTTTGGAAGGTGTTTTTGCACCACCATTCAGGCTTCCAGGCACAAGATTTTCAAGAGCCAAGAAAGCAAGACTGGAGATAGAGACAATGCTAAGGGAAGTGGTGAGAGAGAAGAGGAGAAAAATTGAAGGAAAACTAGAAGAAGAAGAACAATGTGGGTCATTGCTTTCAAGGATGGTGAGAGCAATGATAGGAGGGGAAATCAGTGAAATGGAAGTCGTAGACAATATTGTTTTGCTTGTTTTTGCAGCTCATGATACGACCTCCTTTGCCATTGCCATGACTTTCAAAATGTTGTCTCAGCACACAAACTGCTACTCACTTCTTCTCCAGGAACACGCTGACATAGTAAGAAGCAAGGGGCCAGGCCAGAATTTGACAATGGAAGACATAAAGAAGATGAAATATACTTGGCAGGTAGCTCGGGAGAGCATTAGATTGTTTCCCCCTATATTTGGTTCTTTCAGGAAAGCCATTTCAGACATTGAATACGAGAGCTTTCTCATTCCCAAAGGATGGAAGGTCTTATGGACAACGTACGGAACACATTACGGAGAAGAGTACTTTGGAGATCCATTGAGTTTCAAGCCAAGCAGATTTGAGGAACCTGTTTCTCAGTATGTTTATGTTCCATTTGGAGGAGGGCCAAGAGCTTGTGCAGGCTACCAGCTAGCGAAATTGAACATACTCATCTTTATACACTCGGTTGTAACCCGTTATGATTGGAGTTTAGTTCATCCAGATGAGCCAATCATAATGGATCCTCTCCCTGTCCCTTATCATGGAATGCCTATTAAGATTTCTCCAAAATCAGAAATTTGTCCCAATGTTCACCAAATGTAA

Coding sequence (CDS)

ATGGGTCTACCTTGGATTGGAGAAACAATGGCTTTTTACAGAGCTCAAAGTAAAAACAGATTGTTTGAAGACTTTGTTGAGCCAAGAGTAGCAAAATATGGAAAAATCTTCAAAACAAACTTAATGGGATCTCCAACAGTGGTTGTTAATGGAGCTGAAGCCAATAGATTTTTCTTGTCAAATGAGTTTAAGTTAGTGGTAAGTTCATGGCCATCGAGCTCCGTTCAGTTGATGGGTGAAGAATGTATTATGCAGAAGGAAGGTGAAAAGCATAGATGTGTTCGTGGTCTTATTGCCTCAGCTCTTTCTACTTCGAGTCTTGAAGTTATGATGCCTAAACTGTGCAATACAATTCAGGTTTATTTGGATACTAAGTGGGTTGATTGTGACACAGTTGGTCTCTTTCGCTCGGCTAAAGTATTGACATTTACCATTGTGTTTGAGTGTTTATTGGGTATTATAGTGGAGCCACGTGTGTTAACGACGTTTGAGAGGGTTTTGGAAGGTGTTTTTGCACCACCATTCAGGCTTCCAGGCACAAGATTTTCAAGAGCCAAGAAAGCAAGACTGGAGATAGAGACAATGCTAAGGGAAGTGGTGAGAGAGAAGAGGAGAAAAATTGAAGGAAAACTAGAAGAAGAAGAACAATGTGGGTCATTGCTTTCAAGGATGGTGAGAGCAATGATAGGAGGGGAAATCAGTGAAATGGAAGTCGTAGACAATATTGTTTTGCTTGTTTTTGCAGCTCATGATACGACCTCCTTTGCCATTGCCATGACTTTCAAAATGTTGTCTCAGCACACAAACTGCTACTCACTTCTTCTCCAGGAACACGCTGACATAGTAAGAAGCAAGGGGCCAGGCCAGAATTTGACAATGGAAGACATAAAGAAGATGAAATATACTTGGCAGGTAGCTCGGGAGAGCATTAGATTGTTTCCCCCTATATTTGGTTCTTTCAGGAAAGCCATTTCAGACATTGAATACGAGAGCTTTCTCATTCCCAAAGGATGGAAGGTCTTATGGACAACGTACGGAACACATTACGGAGAAGAGTACTTTGGAGATCCATTGAGTTTCAAGCCAAGCAGATTTGAGGAACCTGTTTCTCAGTATGTTTATGTTCCATTTGGAGGAGGGCCAAGAGCTTGTGCAGGCTACCAGCTAGCGAAATTGAACATACTCATCTTTATACACTCGGTTGTAACCCGTTATGATTGGAGTTTAGTTCATCCAGATGAGCCAATCATAATGGATCCTCTCCCTGTCCCTTATCATGGAATGCCTATTAAGATTTCTCCAAAATCAGAAATTTGTCCCAATGTTCACCAAATGTAA

Protein sequence

MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQVYLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEPRVLTTFERVLEGVFAPPFRLPGTRFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPKSEICPNVHQM
Homology
[Close]
BLAST of Pay0021410 vs. ExPASy Swiss-Prot
Match: Q6WG30 (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2)

HSP 1 Score: 330.9 bits (847), Expect = 2.3e-89
Identity = 176/445 (39.55%), Postives = 274/445 (61.57%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           +G+P+IGE+  F RA   N L E F + RV K+G +FKT+L+G PTVV+ G   NR  LS
Sbjct: 57  LGIPFIGESFIFLRALRSNSL-EQFFDERVKKFGLVFKTSLIGHPTVVLCGPAGNRLILS 116

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           NE KLV  SWP+  ++LMGE  +  + GE H  +R  +A      +L+  + K+   IQ 
Sbjct: 117 NEEKLVQMSWPAQFMKLMGENSVATRRGEDHIVMRSALAGFFGPGALQSYIGKMNTEIQS 176

Query: 121 YLDTKWVDCDTVGLFRSAKVLTF---TIVFECLLGIIVEPRVLTTFERVLEGVFAPPFRL 180
           +++ KW   D V +    + L F    I+F  +     + R+    E +L G FA P  L
Sbjct: 177 HINEKWKGKDEVNVLPLVRELVFNISAILFFNIYDKQEQDRLHKLLETILVGSFALPIDL 236

Query: 181 PGTRFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMV--RAMIGGEISE 240
           PG  F RA + R ++  ++  ++++++  ++       Q   LLS ++  R   G  ++ 
Sbjct: 237 PGFGFHRALQGRAKLNKIMLSLIKKRKEDLQSGSATATQ--DLLSVLLTFRDDKGTPLTN 296

Query: 241 MEVVDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMED 300
            E++DN   L+ A++DTT+  +A+ FK+LS +  CY  ++QE  +I+ +K  G+ +T +D
Sbjct: 297 DEILDNFSSLLHASYDTTTSPMALIFKLLSSNPECYQKVVQEQLEILSNKEEGEEITWKD 356

Query: 301 IKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFG 360
           +K MKYTWQVA+E++R+FPP+FG+FRKAI+DI+Y+ + IPKGWK+LWTTY TH  + YF 
Sbjct: 357 LKAMKYTWQVAQETLRMFPPVFGTFRKAITDIQYDGYTIPKGWKLLWTTYSTHPKDLYFN 416

Query: 361 DPLSFKPSRFEEP---VSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRY-DWSLVH 420
           +P  F PSRF++    V+ Y ++PFGGG R+C G++ +K+ IL+F+H  V  +  ++ V 
Sbjct: 417 EPEKFMPSRFDQEGKHVAPYTFLPFGGGQRSCVGWEFSKMEILLFVHHFVKTFSSYTPVD 476

Query: 421 PDEPIIMDPL-PVPYHGMPIKISPK 436
           PDE I  DPL P+P  G  IK+ P+
Sbjct: 477 PDEKISGDPLPPLPSKGFSIKLFPR 498

[Close]
BLAST of Pay0021410 vs. ExPASy Swiss-Prot
Match: Q8W4T9 (Taxane 13-alpha-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A2 PE=1 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 2.8e-87
Identity = 168/446 (37.67%), Postives = 269/446 (60.31%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           +G P +GET+ F R+   +   + F+E R++K+G +FKT+++G PTVV+ G   NR  LS
Sbjct: 43  LGFPLVGETLQFVRSLGSSTP-QQFIEERMSKFGDVFKTSIIGHPTVVLCGPAGNRLVLS 102

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           NE KLV  SWPSS ++L+GE+C+  K GE+HR VR  +   L   +L+    K+ + IQ 
Sbjct: 103 NENKLVQMSWPSSMMKLIGEDCLGGKTGEQHRIVRAALTRFLGPQALQNHFAKMSSGIQR 162

Query: 121 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGII---VEPRVLTTFERVLEGVFAPPFRL 180
           +++ KW   D   +    K L F++      GI    ++ ++    E +L G F+ P  +
Sbjct: 163 HINEKWKGKDEATVLPLVKDLVFSVASRLFFGITEEHLQEQLHNLLEVILVGSFSVPLNI 222

Query: 181 PGTRFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAM--IGGEISE 240
           PG  + +A +AR  +  ++  ++ ++R ++      E Q   LLS ++      G  +++
Sbjct: 223 PGFSYHKAIQARATLADIMTHLIEKRRNELRAGTASENQ--DLLSVLLTFTDERGNSLAD 282

Query: 241 MEVVDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMED 300
            E++DN  +L+  ++D+T+  + M  K+L+ H   Y  + QE   I+ +K  G+ +  +D
Sbjct: 283 KEILDNFSMLLHGSYDSTNSPLTMLIKVLASHPESYEKVAQEQFGILSTKMEGEEIAWKD 342

Query: 301 IKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFG 360
           +K+MKY+WQV +E++R++PPIFG+FRKAI+DI Y  + IPKGWK+LWTTY T   EEYF 
Sbjct: 343 LKEMKYSWQVVQETLRMYPPIFGTFRKAITDIHYNGYTIPKGWKLLWTTYSTQTKEEYFK 402

Query: 361 DPLSFKPSRFEEP---VSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYD-WSLVH 420
           D   FKPSRFEE    V+ Y Y+PFGGG R C G++ AK+  L+F+H  V  +     + 
Sbjct: 403 DADQFKPSRFEEEGKHVTPYTYLPFGGGMRVCPGWEFAKMETLLFLHHFVKAFSGLKAID 462

Query: 421 PDEPIIMDPL-PVPYHGMPIKISPKS 437
           P+E +   PL P+P +G+PIK+  +S
Sbjct: 463 PNEKLSGKPLPPLPVNGLPIKLYSRS 485

[Close]
BLAST of Pay0021410 vs. ExPASy Swiss-Prot
Match: Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 5.2e-86
Identity = 175/442 (39.59%), Postives = 258/442 (58.37%), Query Frame = 0 

Query: 2   GLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLSN 61
           G P IGET+ F   Q K     DF + R  KYG +F T+L+G PTVV    E NRF  SN
Sbjct: 57  GWPLIGETLEFLGCQRKGNP-RDFFDSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSN 116

Query: 62  EFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQVY 121
           E KLVV+SWPSS   L     ++   G+  + +R ++ + L   +L   + ++ +  + +
Sbjct: 117 ENKLVVNSWPSSVGNLF-RSSLITTVGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRH 176

Query: 122 LDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEP---RVLTTFERVLEGVFAPPFRLP 181
           L   W+  D V      K  TF++  +    I  +    R+   F   ++GV   P  LP
Sbjct: 177 LAEHWIGKDEVTALPLLKRYTFSLACDLFASINNKDDLGRLWLHFMVFVKGVMQIPIDLP 236

Query: 182 GTRFSRAKKARLEIETMLREVVREKRRKIE-GKLEEEEQCGSLLSRMVRAMIGGEISEME 241
           GTR+++AK A   I   L  ++ E++  +E G    E+   S L   V    G  +++ E
Sbjct: 237 GTRYNKAKHAANAIRQQLGSIINERKIGLEAGNASPEQDLLSFLLSNVDEQ-GESLTDNE 296

Query: 242 VVDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIK 301
           + DNI+LL++A HDT+S  + +  K L+++ +CY  +L+E  DI  SK  GQ L  ED++
Sbjct: 297 IQDNILLLLYAGHDTSSSTLTVLLKFLAENPHCYEEVLREQLDIAGSKEAGQLLEWEDLQ 356

Query: 302 KMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDP 361
           +MKY+W+VA+E++RLFP   GSFRKAI +  Y+ F IPKGWK+ WT   TH   EYF +P
Sbjct: 357 RMKYSWRVAQEALRLFPAAQGSFRKAIKEFIYDGFTIPKGWKMYWTVNSTHRKSEYFSNP 416

Query: 362 LSFKPSRFE-EPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPI 421
            +F PSRFE E    Y +VPFGGGPR C G + A+L IL+F+H++V    W+LV+P E +
Sbjct: 417 ETFDPSRFEGEGPPPYTFVPFGGGPRMCPGNEFARLEILVFLHNIVKNCKWNLVNPGEKV 476

Query: 422 IMDPLPVPYHGMPIKISPKSEI 439
           I+DP+P P +G+PIK+ P   +
Sbjct: 477 IVDPMPAPVNGLPIKLVPHDSV 495

[Close]
BLAST of Pay0021410 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 2.0e-85
Identity = 173/438 (39.50%), Postives = 258/438 (58.90%), Query Frame = 0 

Query: 2   GLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLSN 61
           G P IGET+ F   Q K     DF + R  KYG +F T+L+G PTVV    E NRF  SN
Sbjct: 57  GWPLIGETLEFLGCQRKGNP-RDFFDSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSN 116

Query: 62  EFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQVY 121
           E KLVV+SWPSS   L     ++   G+  + +R ++ + L   +L   + ++ +  + +
Sbjct: 117 ENKLVVNSWPSSVGNLF-RSSLITTVGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRH 176

Query: 122 LDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVE---PRVLTTFERVLEGVFAPPFRLP 181
           L   W+  D V      K  TF++  +    I  +    R+   F   ++GV   P  LP
Sbjct: 177 LAEHWIGKDEVMALPLLKRYTFSLACDLFASINTKDDLDRLWLHFMVFVKGVMQIPIDLP 236

Query: 182 GTRFSRAKKARLEIETMLREVVREKRRKIE-GKLEEEEQCGSLLSRMVRAMIGGEISEME 241
           GTR+++ K A   I   L  ++ E++  +E G    E+   S L   V    G  +++ E
Sbjct: 237 GTRYNKTKHAANAIRQQLGSIINERKIALEAGNASPEQDLLSFLLSNVDEQ-GESLTDNE 296

Query: 242 VVDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIK 301
           + DNI+LL++A HDT+S  + +  K L+++ +CY  +L+E  +I  SK  GQ L  ED++
Sbjct: 297 IQDNILLLLYAGHDTSSSTLTVLLKFLAENPHCYEEVLREQLNIAGSKEEGQLLEWEDLQ 356

Query: 302 KMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDP 361
           +MKY+W+VA+E++RLFP + GSFRKAI +  Y+ F IPKGWK+ WT   TH   EYF +P
Sbjct: 357 RMKYSWRVAQEALRLFPAVQGSFRKAIKEFIYDGFTIPKGWKLHWTVNSTHQKSEYFSNP 416

Query: 362 LSFKPSRFE-EPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPI 421
             F PSRFE E    Y +VPFGGGPR C G + A++ ILIF+H++V  ++W+LV+P E +
Sbjct: 417 EKFDPSRFEGEGPPPYTFVPFGGGPRMCPGNEFARMEILIFLHNIVKNFNWNLVNPLEKV 476

Query: 422 IMDPLPVPYHGMPIKISP 435
           I+DP+P P +G+PIK+ P
Sbjct: 477 IVDPMPAPVNGLPIKLVP 491

[Close]
BLAST of Pay0021410 vs. ExPASy Swiss-Prot
Match: Q84KI1 (Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.9e-84
Identity = 172/445 (38.65%), Postives = 262/445 (58.88%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           +G P+IGE++ F +A   N + E F++ RV  +G +FKT+L+G PTVV+ G   NR  L+
Sbjct: 55  LGYPFIGESLLFLKALRSNTV-EQFLDERVKNFGNVFKTSLIGHPTVVLCGPAGNRLILA 114

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           NE KLV  SWP SS++LMGE+ I  K GE H  +R  +    S  +L+  + ++  TI+ 
Sbjct: 115 NEEKLVQMSWPKSSMKLMGEKSITAKRGEGHMIIRSALQGFFSPGALQKYIGQMSKTIEN 174

Query: 121 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVE----PRVLTTFERVLEGVFAPPFR 180
           +++ KW   D V +      L F I   CL   I E     R+    E +  GV A P  
Sbjct: 175 HINEKWKGNDQVSVVALVGDLVFDI-SACLFFNINEKHERERLFELLEIIAVGVLAVPVD 234

Query: 181 LPGTRFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEM 240
           LPG  + RA +AR ++  +L  ++ +++  +   L    Q    +    +   G   S+ 
Sbjct: 235 LPGFAYHRALQARSKLNAILSGLIEKRKMDLSSGLATSNQDLLSVFLTFKDDRGNPCSDE 294

Query: 241 EVVDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDI 300
           E++DN   L+  ++DTT  A+A  FK+LS +  CY  ++QE   I+ +K  G  +T +D+
Sbjct: 295 EILDNFSGLLHGSYDTTVSAMACVFKLLSSNPECYEKVVQEQLGILSNKLEGDEITWKDV 354

Query: 301 KKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGD 360
           K MKYTWQV +E++RL+P IFGSFR+AI+DI Y  ++IPKGWK+LWT Y TH  E YF +
Sbjct: 355 KSMKYTWQVVQETLRLYPSIFGSFRQAITDIHYNGYIIPKGWKLLWTPYTTHPKEMYFSE 414

Query: 361 PLSFKPSRFEEP---VSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYD-WSLVHP 420
           P  F PSRF++    V+ Y ++PFGGG R+C G++ +K+ IL+ +H  V  +  ++ V P
Sbjct: 415 PEKFLPSRFDQEGKLVAPYTFLPFGGGQRSCPGWEFSKMEILLSVHHFVKTFSTFTPVDP 474

Query: 421 DEPIIMDPL-PVPYHGMPIKISPKS 437
            E I  D L P+P +G  +K+ P+S
Sbjct: 475 AEIIARDSLCPLPSNGFSVKLFPRS 497

[Close]
BLAST of Pay0021410 vs. ExPASy TrEMBL
Match: A0A1S3BLK9 (taxadiene 5-alpha hydroxylase OS=Cucumis melo OX=3656 GN=LOC103491382 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.2e-255
Identity = 442/445 (99.33%), Postives = 442/445 (99.33%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS
Sbjct: 32  MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 91

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV
Sbjct: 92  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 151

Query: 121 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEPRVLTTFERVLEGVFAPPFRLPGT 180
           YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGI VEPRVLTTFERVLEGVFAPPFR PGT
Sbjct: 152 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIKVEPRVLTTFERVLEGVFAPPFRFPGT 211

Query: 181 RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD 240
           RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD
Sbjct: 212 RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD 271

Query: 241 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 300
           NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK
Sbjct: 272 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 331

Query: 301 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF 360
           YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF
Sbjct: 332 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF 391

Query: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP 420
           KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP
Sbjct: 392 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP 451

Query: 421 LPVPYHGMPIKISPKSEICPNVHQM 446
           LPVP HGMPIKISPKSEICPNVHQM
Sbjct: 452 LPVPCHGMPIKISPKSEICPNVHQM 476

[Close]
BLAST of Pay0021410 vs. ExPASy TrEMBL
Match: A0A0A0KEB2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G423370 PE=3 SV=1)

HSP 1 Score: 862.1 bits (2226), Expect = 1.0e-246
Identity = 424/445 (95.28%), Postives = 433/445 (97.30%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETM FYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS
Sbjct: 1   MGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIA+ LSTSSL+VMMPKLCNTIQV
Sbjct: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIAATLSTSSLDVMMPKLCNTIQV 120

Query: 121 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEPRVLTTFERVLEGVFAPPFRLPGT 180
           YLDTKWV CDT+GLF SAKVLTFTIVFECLLGI VEPRVLT FERVLEGVFAPPFR PGT
Sbjct: 121 YLDTKWVGCDTIGLFHSAKVLTFTIVFECLLGIQVEPRVLTMFERVLEGVFAPPFRFPGT 180

Query: 181 RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD 240
           RFSRAKKARLEIET LREVVREKRRKIEGKLEEEE CGSLLSRMVRAMIGGEISEMEV+D
Sbjct: 181 RFSRAKKARLEIETTLREVVREKRRKIEGKLEEEE-CGSLLSRMVRAMIGGEISEMEVID 240

Query: 241 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 300
           NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCY+LLLQEH DI+RSKGPGQNLTMEDIKKMK
Sbjct: 241 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMK 300

Query: 301 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF 360
           YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYF DPLSF
Sbjct: 301 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSF 360

Query: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP 420
           KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIF+HSVVTRYDWSLVHPDEPIIMDP
Sbjct: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFVHSVVTRYDWSLVHPDEPIIMDP 420

Query: 421 LPVPYHGMPIKISPKSEICPNVHQM 446
           LPVPYHGMPIKISPKS++C NVHQM
Sbjct: 421 LPVPYHGMPIKISPKSQVCSNVHQM 444

[Close]
BLAST of Pay0021410 vs. ExPASy TrEMBL
Match: A0A6J1GL90 (taxadiene 5-alpha hydroxylase isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111455006 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.4e-219
Identity = 383/445 (86.07%), Postives = 408/445 (91.69%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETM FYRAQ KNRLFE+FV PRVAKYGKIFKT+LMGSPTVVVNGAEANRFFLS
Sbjct: 1   MGLPWIGETMVFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           NEFKLVVSSWPS+SVQLMGEECIMQK+GE HRCVRGLIA++L++SSLE MMPKLC TIQ+
Sbjct: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120

Query: 121 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEPRVLTTFERVLEGVFAPPFRLPGT 180
           YLDTKW+  D + L+ SAKVLTFTIVFECL+GI VEPR+LTTFERVLEGVFAPPF  PGT
Sbjct: 121 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 180

Query: 181 RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD 240
           RFSRAKKAR+EI  MLREVVREKRR+IE +  EEE C  LLSRMVRAMI GEISE+EVVD
Sbjct: 181 RFSRAKKARMEIGKMLREVVREKRRRIEEEPVEEE-CELLLSRMVRAMIRGEISEVEVVD 240

Query: 241 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 300
           N+VLLVFAAHDTTSFAIAMTFKML+Q  NCYSLLLQEHADIVRSKG GQN+TMEDIKKMK
Sbjct: 241 NVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMK 300

Query: 301 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF 360
           YTWQVARESIRLFPPIFGSFRKAI+DIEYE F IPKGWKVLWTTYGTH  ++YF DPLSF
Sbjct: 301 YTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDKYFEDPLSF 360

Query: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP 420
           KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIH VVTRYDWSLVHPDE IIMDP
Sbjct: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDP 420

Query: 421 LPVPYHGMPIKISPKSEICPNVHQM 446
           LP+P HGMPIKISPKSE  PNV QM
Sbjct: 421 LPIPSHGMPIKISPKSEARPNVQQM 444

[Close]
BLAST of Pay0021410 vs. ExPASy TrEMBL
Match: A0A6J1GK43 (taxadiene 5-alpha hydroxylase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111455006 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.4e-219
Identity = 383/445 (86.07%), Postives = 408/445 (91.69%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETM FYRAQ KNRLFE+FV PRVAKYGKIFKT+LMGSPTVVVNGAEANRFFLS
Sbjct: 31  MGLPWIGETMVFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 90

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           NEFKLVVSSWPS+SVQLMGEECIMQK+GE HRCVRGLIA++L++SSLE MMPKLC TIQ+
Sbjct: 91  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 150

Query: 121 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEPRVLTTFERVLEGVFAPPFRLPGT 180
           YLDTKW+  D + L+ SAKVLTFTIVFECL+GI VEPR+LTTFERVLEGVFAPPF  PGT
Sbjct: 151 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 210

Query: 181 RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD 240
           RFSRAKKAR+EI  MLREVVREKRR+IE +  EEE C  LLSRMVRAMI GEISE+EVVD
Sbjct: 211 RFSRAKKARMEIGKMLREVVREKRRRIEEEPVEEE-CELLLSRMVRAMIRGEISEVEVVD 270

Query: 241 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 300
           N+VLLVFAAHDTTSFAIAMTFKML+Q  NCYSLLLQEHADIVRSKG GQN+TMEDIKKMK
Sbjct: 271 NVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMK 330

Query: 301 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF 360
           YTWQVARESIRLFPPIFGSFRKAI+DIEYE F IPKGWKVLWTTYGTH  ++YF DPLSF
Sbjct: 331 YTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDKYFEDPLSF 390

Query: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP 420
           KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIH VVTRYDWSLVHPDE IIMDP
Sbjct: 391 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDP 450

Query: 421 LPVPYHGMPIKISPKSEICPNVHQM 446
           LP+P HGMPIKISPKSE  PNV QM
Sbjct: 451 LPIPSHGMPIKISPKSEARPNVQQM 474

[Close]
BLAST of Pay0021410 vs. ExPASy TrEMBL
Match: A0A6J1GJY9 (taxadiene 5-alpha hydroxylase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455006 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.4e-219
Identity = 383/445 (86.07%), Postives = 408/445 (91.69%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETM FYRAQ KNRLFE+FV PRVAKYGKIFKT+LMGSPTVVVNGAEANRFFLS
Sbjct: 43  MGLPWIGETMVFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 102

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           NEFKLVVSSWPS+SVQLMGEECIMQK+GE HRCVRGLIA++L++SSLE MMPKLC TIQ+
Sbjct: 103 NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 162

Query: 121 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEPRVLTTFERVLEGVFAPPFRLPGT 180
           YLDTKW+  D + L+ SAKVLTFTIVFECL+GI VEPR+LTTFERVLEGVFAPPF  PGT
Sbjct: 163 YLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 222

Query: 181 RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD 240
           RFSRAKKAR+EI  MLREVVREKRR+IE +  EEE C  LLSRMVRAMI GEISE+EVVD
Sbjct: 223 RFSRAKKARMEIGKMLREVVREKRRRIEEEPVEEE-CELLLSRMVRAMIRGEISEVEVVD 282

Query: 241 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 300
           N+VLLVFAAHDTTSFAIAMTFKML+Q  NCYSLLLQEHADIVRSKG GQN+TMEDIKKMK
Sbjct: 283 NVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMK 342

Query: 301 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF 360
           YTWQVARESIRLFPPIFGSFRKAI+DIEYE F IPKGWKVLWTTYGTH  ++YF DPLSF
Sbjct: 343 YTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDKYFEDPLSF 402

Query: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP 420
           KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIH VVTRYDWSLVHPDE IIMDP
Sbjct: 403 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDP 462

Query: 421 LPVPYHGMPIKISPKSEICPNVHQM 446
           LP+P HGMPIKISPKSE  PNV QM
Sbjct: 463 LPIPSHGMPIKISPKSEARPNVQQM 486

[Close]
BLAST of Pay0021410 vs. NCBI nr
Match: XP_008449518.1 (PREDICTED: taxadiene 5-alpha hydroxylase [Cucumis melo])

HSP 1 Score: 891.7 bits (2303), Expect = 2.5e-255
Identity = 442/445 (99.33%), Postives = 442/445 (99.33%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS
Sbjct: 32  MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 91

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV
Sbjct: 92  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 151

Query: 121 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEPRVLTTFERVLEGVFAPPFRLPGT 180
           YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGI VEPRVLTTFERVLEGVFAPPFR PGT
Sbjct: 152 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIKVEPRVLTTFERVLEGVFAPPFRFPGT 211

Query: 181 RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD 240
           RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD
Sbjct: 212 RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD 271

Query: 241 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 300
           NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK
Sbjct: 272 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 331

Query: 301 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF 360
           YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF
Sbjct: 332 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF 391

Query: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP 420
           KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP
Sbjct: 392 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP 451

Query: 421 LPVPYHGMPIKISPKSEICPNVHQM 446
           LPVP HGMPIKISPKSEICPNVHQM
Sbjct: 452 LPVPCHGMPIKISPKSEICPNVHQM 476

[Close]
BLAST of Pay0021410 vs. NCBI nr
Match: XP_004140249.1 (taxadiene 5-alpha hydroxylase [Cucumis sativus] >KAE8647348.1 hypothetical protein Csa_003098 [Cucumis sativus])

HSP 1 Score: 862.1 bits (2226), Expect = 2.2e-246
Identity = 424/445 (95.28%), Postives = 433/445 (97.30%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETM FYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS
Sbjct: 32  MGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 91

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIA+ LSTSSL+VMMPKLCNTIQV
Sbjct: 92  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIAATLSTSSLDVMMPKLCNTIQV 151

Query: 121 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEPRVLTTFERVLEGVFAPPFRLPGT 180
           YLDTKWV CDT+GLF SAKVLTFTIVFECLLGI VEPRVLT FERVLEGVFAPPFR PGT
Sbjct: 152 YLDTKWVGCDTIGLFHSAKVLTFTIVFECLLGIQVEPRVLTMFERVLEGVFAPPFRFPGT 211

Query: 181 RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD 240
           RFSRAKKARLEIET LREVVREKRRKIEGKLEEEE CGSLLSRMVRAMIGGEISEMEV+D
Sbjct: 212 RFSRAKKARLEIETTLREVVREKRRKIEGKLEEEE-CGSLLSRMVRAMIGGEISEMEVID 271

Query: 241 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 300
           NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCY+LLLQEH DI+RSKGPGQNLTMEDIKKMK
Sbjct: 272 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMK 331

Query: 301 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF 360
           YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYF DPLSF
Sbjct: 332 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSF 391

Query: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP 420
           KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIF+HSVVTRYDWSLVHPDEPIIMDP
Sbjct: 392 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFVHSVVTRYDWSLVHPDEPIIMDP 451

Query: 421 LPVPYHGMPIKISPKSEICPNVHQM 446
           LPVPYHGMPIKISPKS++C NVHQM
Sbjct: 452 LPVPYHGMPIKISPKSQVCSNVHQM 475

[Close]
BLAST of Pay0021410 vs. NCBI nr
Match: XP_038888983.1 (taxadiene 5-alpha hydroxylase [Benincasa hispida])

HSP 1 Score: 803.9 bits (2075), Expect = 7.0e-229
Identity = 397/445 (89.21%), Postives = 416/445 (93.48%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKT+LMGSPTVVVNGAEANRFFLS
Sbjct: 31  MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 90

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           NEFKLVVSSWPS+SVQLMGEECIMQK+GE HR VR LIA++LSTSSLEVMMPKL  TI+V
Sbjct: 91  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRFVRSLIAASLSTSSLEVMMPKLFTTIRV 150

Query: 121 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEPRVLTTFERVLEGVFAPPFRLPGT 180
           YLDTKWV  DT+GLF SAKVLTFT+VFECL+GI VEPRVLTTFERVLEGVFAPP R PGT
Sbjct: 151 YLDTKWVGRDTIGLFHSAKVLTFTVVFECLMGIKVEPRVLTTFERVLEGVFAPPIRFPGT 210

Query: 181 RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD 240
           RFSRAKKARLEIE MLREVVREKRR+IEGKLEEEE+CGSLLSRMVRAMI GEISEMEV+D
Sbjct: 211 RFSRAKKARLEIEKMLREVVREKRRRIEGKLEEEEECGSLLSRMVRAMIRGEISEMEVID 270

Query: 241 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 300
           N+VL VFAAHDTTSFAIAMTFKML+ HTNCYSLLLQE  DI RSKG  QNLTMEDIKKMK
Sbjct: 271 NVVLFVFAAHDTTSFAIAMTFKMLAHHTNCYSLLLQEQTDIARSKGSNQNLTMEDIKKMK 330

Query: 301 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF 360
           YTWQVARESIRLFPPIFGSFRKAISDIEYE F IP+GWKVLWTTYGTHY EEYF DPLSF
Sbjct: 331 YTWQVARESIRLFPPIFGSFRKAISDIEYERFFIPEGWKVLWTTYGTHYKEEYFEDPLSF 390

Query: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP 420
           KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIH VVTRYDW+LVHPDEPIIMDP
Sbjct: 391 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWNLVHPDEPIIMDP 450

Query: 421 LPVPYHGMPIKISPKSEICPNVHQM 446
           LPVP  GMP+KIS KS++CPN HQM
Sbjct: 451 LPVPSQGMPMKISLKSDVCPNDHQM 475

[Close]
BLAST of Pay0021410 vs. NCBI nr
Match: KAG6572434.1 (hypothetical protein SDJN03_29162, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 780.0 bits (2013), Expect = 1.1e-221
Identity = 387/445 (86.97%), Postives = 411/445 (92.36%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETMAFYRAQ KNRLFE+FV PRVAKYGKIFKT+LMGSPTVVVNGAEANRFFLS
Sbjct: 1   MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 60

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           NEFKLVVSSWPS+SVQLMGEECIMQK+GE HRCVRGLIA++L++SSLE MMPKLC TIQ+
Sbjct: 61  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQL 120

Query: 121 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEPRVLTTFERVLEGVFAPPFRLPGT 180
           YLDTKWV  D + L+ SAKVLTFTIVFECL+GI VEPR+LTTFERVLEGVFAPPF  PGT
Sbjct: 121 YLDTKWVGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 180

Query: 181 RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD 240
           RFSRAKKAR+EI  MLREVVREKRR+IE + E EE C  LLSRMVRAMI GEISE+EVVD
Sbjct: 181 RFSRAKKARMEIGKMLREVVREKRRRIEEEPEGEE-CELLLSRMVRAMIRGEISEVEVVD 240

Query: 241 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 300
           N+VLLVFAAHDTTSFAIA+TFKML+Q  NCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK
Sbjct: 241 NVVLLVFAAHDTTSFAIAVTFKMLAQQPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 300

Query: 301 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF 360
           YTWQVARESIRLFPPIFGSFRKAI+DIEYE F IPKGWKVLWTTYGTH  +EYF DPLSF
Sbjct: 301 YTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSF 360

Query: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP 420
           KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIH VVTRYDWSL+HPDEPIIMDP
Sbjct: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDP 420

Query: 421 LPVPYHGMPIKISPKSEICPNVHQM 446
           LP+P HGMPIKISPKSE  PNV QM
Sbjct: 421 LPIPSHGMPIKISPKSESHPNVQQM 444

[Close]
BLAST of Pay0021410 vs. NCBI nr
Match: KAG7012036.1 (hypothetical protein SDJN02_26944, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 778.5 bits (2009), Expect = 3.1e-221
Identity = 386/445 (86.74%), Postives = 410/445 (92.13%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETMAFYRAQ KNRLFE+FV PRVAKYGKIFKT+LMGSPTVVVNGAEANRFFLS
Sbjct: 43  MGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 102

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           NEFKLVVSSWPS+SVQLMGEECIMQK+GE HRC RGLIA++L++SSLE MMPKLC TIQ+
Sbjct: 103 NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCARGLIAASLASSSLEAMMPKLCTTIQL 162

Query: 121 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEPRVLTTFERVLEGVFAPPFRLPGT 180
           YLDTKWV  D + L+ SAKVLTFTIVFECL+GI VEPR+LTTFERVLEGVFAPPF  PGT
Sbjct: 163 YLDTKWVGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGT 222

Query: 181 RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD 240
           RFSRAKKAR+EI  MLREVVREKRR+IE + E EE C  LLSRMVRAMI GEISE+EVVD
Sbjct: 223 RFSRAKKARMEIGKMLREVVREKRRRIEEEPEGEE-CELLLSRMVRAMIRGEISEVEVVD 282

Query: 241 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 300
           N+VLLVFAAHDTTSFAIA+TFKML+Q  NCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK
Sbjct: 283 NVVLLVFAAHDTTSFAIAVTFKMLAQQPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 342

Query: 301 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF 360
           YTWQVARESIRLFPPIFGSFRKAI+DIEYE F IPKGWKVLWTTYGTH  +EYF DPLSF
Sbjct: 343 YTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSF 402

Query: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP 420
           KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIH VVTRYDWSL+HPDEPIIMDP
Sbjct: 403 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDP 462

Query: 421 LPVPYHGMPIKISPKSEICPNVHQM 446
           LP+P HGMPIKISPKSE  PNV QM
Sbjct: 463 LPIPSHGMPIKISPKSESHPNVQQM 486

[Close]
BLAST of Pay0021410 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 589.3 bits (1518), Expect = 2.5e-168
Identity = 286/435 (65.75%), Postives = 351/435 (80.69%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           MGLPWIGETM FY+AQ  NR+FEDFV PR+ K+G IFKT +MGSPT+VVNGAEANR  LS
Sbjct: 50  MGLPWIGETMDFYKAQKSNRVFEDFVNPRIIKHGNIFKTRIMGSPTIVVNGAEANRLILS 109

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           NEF LVVSSWPSSSVQLMG  CIM K+GEKHR +RG++A++LS   LE ++PKLC+T++ 
Sbjct: 110 NEFSLVVSSWPSSSVQLMGMNCIMAKQGEKHRVLRGIVANSLSYIGLESLIPKLCDTVKF 169

Query: 121 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEPRVLTTFERVLEGVFAPPFRLPGT 180
           + +T+W   + + L+RSAKVLTFT+VFECL GI VE  +L  FERVLEGVFA P   P +
Sbjct: 170 HHETEWRGKEEISLYRSAKVLTFTVVFECLYGIKVEIGMLEVFERVLEGVFALPVEFPCS 229

Query: 181 RFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEISEMEVVD 240
           +F+RAKKARLEIET L   VREKRR++E K   E+   +L SR+V  +I G I+E EVVD
Sbjct: 230 KFARAKKARLEIETFLVGKVREKRREME-KEGAEKPNTTLFSRLVEELIKGVITEEEVVD 289

Query: 241 NIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMK 300
           N+VLLVFAAHDTTS+A++MTFKML+QH  C   LLQEHA I  +KG G+ LT+ED+KKMK
Sbjct: 290 NMVLLVFAAHDTTSYAMSMTFKMLAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMK 349

Query: 301 YTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFGDPLSF 360
           Y+WQV RE++RL PPIFGSFRKA++DI+Y  + IPKGWK+LWTTYGTHY  E F DP+SF
Sbjct: 350 YSWQVVRETMRLSPPIFGSFRKAVADIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSF 409

Query: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEPIIMDP 420
            P+RF++P+  Y Y+PFGGGPR CAG+QLAK++IL+F+H VVT +DWSLV+PDE I MDP
Sbjct: 410 DPTRFDKPIQAYTYLPFGGGPRLCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDP 469

Query: 421 LPVPYHGMPIKISPK 436
           LP P  GMPIKISPK
Sbjct: 470 LPFPSLGMPIKISPK 483

[Close]
BLAST of Pay0021410 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 294.3 bits (752), Expect = 1.7e-79
Identity = 170/445 (38.20%), Postives = 253/445 (56.85%), Query Frame = 0 

Query: 2   GLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGK----IFKTNLMGSPTVVVNGAEANRF 61
           GLP IGE+ +F  A  +    E F+  RV ++      +FKT+L GSPT VV GA  N+F
Sbjct: 40  GLPLIGESFSFLSAGRQGHP-EKFITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKF 99

Query: 62  FLSNEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNT 121
             +NE KLVVS WP  SV  +    +     E+ R +R L++  +   +L   +  +   
Sbjct: 100 LFTNENKLVVSWWP-DSVNKIFPSSMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEI 159

Query: 122 IQVYLDTKWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEPRVL---TTFERVLEGVFAPP 181
            Q + +T+W + D V +F   K  TF+I     L +    RV      F  V  G+F+ P
Sbjct: 160 AQRHFETEWANQDQVIVFPLTKKFTFSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIP 219

Query: 182 FRLPGTRFSRAKKARLEIETMLREVVREKRRKIE-GKLEEEEQCGSLLSRMVRAMIGGEI 241
             LPGTRF+RA KA   +   +  +VR+++ +++ GK  EE     +LS M+  M  GE 
Sbjct: 220 IDLPGTRFNRAIKASRLLRKEVSAIVRQRKEELKAGKALEEH---DILSHML--MNIGET 279

Query: 242 SEMEVVDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTM 301
            + ++ D I+ L+   HDT S         L++  + Y  +LQE  +I++ K   + L  
Sbjct: 280 KDEDLADKIIGLLIGGHDTASIVCTFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRW 339

Query: 302 EDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEY 361
           EDI+KM+Y+W VA E +R+ PP+ G+FR+AI    ++ F IPKGWK+ W+   TH   +Y
Sbjct: 340 EDIEKMRYSWNVACEVMRIVPPLSGTFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDY 399

Query: 362 FGDPLSFKPSRFE-EPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHP 421
           F +P  F+P+RFE      Y YVPFGGGPR C G + A+L ILIF+H++V R+ W  V P
Sbjct: 400 FPEPERFEPNRFEGSGPKPYTYVPFGGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFP 459

Query: 422 DE-PIIMDPLPVPYHGMPIKISPKS 437
           +E  I++DPLP+P  G+PI+I P+S
Sbjct: 460 NENKIVVDPLPIPDKGLPIRIFPQS 477

[Close]
BLAST of Pay0021410 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 247.3 bits (630), Expect = 2.3e-65
Identity = 149/441 (33.79%), Postives = 233/441 (52.83%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           MGLP+IGET+  Y     +     F   R  KYG IFKT+++G P V+++  EA R  L 
Sbjct: 54  MGLPYIGETLRLYTENPNS-----FFATRQNKYGDIFKTHILGCPCVMISSPEAARMVLV 113

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           ++  L   ++P S  +++G E +   +G  H  ++ L+ S+   S+L    P + +   +
Sbjct: 114 SKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSALR---PTVSHIELL 173

Query: 121 YLDT--KWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEPRVLTT----FERVLEGVFAPP 180
            L T   W    ++      K   F +      G   EP  +      ++R+  G  + P
Sbjct: 174 VLQTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMP 233

Query: 181 FRLPGTRFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEIS 240
             LPGT F ++ KAR+E+   LR+V+ EKRR  E   EE    G LL    +   G  +S
Sbjct: 234 LDLPGTLFHKSMKARIELSEELRKVI-EKRR--ENGREEGGLLGVLLGAKDQKRNG--LS 293

Query: 241 EMEVVDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADI-VRSKGPGQNLTM 300
           + ++ DNI+ ++FAA DTT+  +    K L  H N    + +E   I  + K   + ++ 
Sbjct: 294 DSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISW 353

Query: 301 EDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEY 360
           ED +KM  T +V +E++R    +  +FR+A+ D+EY+ +LIPKGWKVL      H+  E+
Sbjct: 354 EDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEF 413

Query: 361 FGDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPD 420
           F DP  F PSRFE     Y Y+PFG G  +C G +LAKL +LI +H + T + W ++  +
Sbjct: 414 FPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDE 473

Query: 421 EPIIMDPLPVPYHGMPIKISP 435
           E I   P PVP  G+PI+++P
Sbjct: 474 EGIQYGPFPVPKKGLPIRVTP 481

[Close]
BLAST of Pay0021410 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 247.3 bits (630), Expect = 2.3e-65
Identity = 149/441 (33.79%), Postives = 233/441 (52.83%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           MGLP+IGET+  Y     +     F   R  KYG IFKT+++G P V+++  EA R  L 
Sbjct: 54  MGLPYIGETLRLYTENPNS-----FFATRQNKYGDIFKTHILGCPCVMISSPEAARMVLV 113

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
           ++  L   ++P S  +++G E +   +G  H  ++ L+ S+   S+L    P + +   +
Sbjct: 114 SKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSALR---PTVSHIELL 173

Query: 121 YLDT--KWVDCDTVGLFRSAKVLTFTIVFECLLGIIVEPRVLTT----FERVLEGVFAPP 180
            L T   W    ++      K   F +      G   EP  +      ++R+  G  + P
Sbjct: 174 VLQTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMP 233

Query: 181 FRLPGTRFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGGEIS 240
             LPGT F ++ KAR+E+   LR+V+ EKRR  E   EE    G LL    +   G  +S
Sbjct: 234 LDLPGTLFHKSMKARIELSEELRKVI-EKRR--ENGREEGGLLGVLLGAKDQKRNG--LS 293

Query: 241 EMEVVDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADI-VRSKGPGQNLTM 300
           + ++ DNI+ ++FAA DTT+  +    K L  H N    + +E   I  + K   + ++ 
Sbjct: 294 DSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISW 353

Query: 301 EDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEY 360
           ED +KM  T +V +E++R    +  +FR+A+ D+EY+ +LIPKGWKVL      H+  E+
Sbjct: 354 EDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEF 413

Query: 361 FGDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPD 420
           F DP  F PSRFE     Y Y+PFG G  +C G +LAKL +LI +H + T + W ++  +
Sbjct: 414 FPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDE 473

Query: 421 EPIIMDPLPVPYHGMPIKISP 435
           E I   P PVP  G+PI+++P
Sbjct: 474 EGIQYGPFPVPKKGLPIRVTP 481

[Close]
BLAST of Pay0021410 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 243.0 bits (619), Expect = 4.4e-64
Identity = 139/443 (31.38%), Postives = 242/443 (54.63%), Query Frame = 0 

Query: 1   MGLPWIGETMAFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60
           MG P++GET   Y +Q  N  F+     +  +YG +FKT+++G P V+++  EA +F L 
Sbjct: 41  MGWPYVGETFQLY-SQDPNVFFQS----KQKRYGSVFKTHVLGCPCVMISSPEAAKFVLV 100

Query: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIASALSTSSLEVMMPKLCNTIQV 120
            +  L   ++P+S  +++G++ I   +G+ H  +R L+  A    S+  M+P + +  Q 
Sbjct: 101 TKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIESIAQD 160

Query: 121 YLDTKWVDCDTVGLFRSAKVLTFTIVFECLLG---IIVEPRVLTTFERVLEGVFAPPFRL 180
            L   W +   +  ++  K  TF +    + G   ++    +   +  + +G  + P  L
Sbjct: 161 SL-RSW-EGTMINTYQEMKTYTFNVALLSIFGKDEVLYREDLKRCYYILEKGYNSMPVNL 220

Query: 181 PGTRFSRAKKARLEIETMLREVVREKRRKIEGKLEEEEQCGSLLSRMVRAMIGG--EISE 240
           PGT F ++ KAR E+  +L  ++ E+R           Q GS  + ++ + +G   E+++
Sbjct: 221 PGTLFHKSMKARKELSQILARILSERR-----------QNGSSHNDLLGSFMGDKEELTD 280

Query: 241 MEVVDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYSLLLQEHADIVRSKGPGQNLTMED 300
            ++ DNI+ ++FAA DTT+  ++   K L+++ N    + +E   I + K  G++LT  D
Sbjct: 281 EQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGD 340

Query: 301 IKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFG 360
            KKM  T +V +E++R+   +  +FR+A+ D+EYE +LIPKGWKVL      H+  + F 
Sbjct: 341 TKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHSADIFS 400

Query: 361 DPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSLVHPDEP 420
           +P  F PSRFE       ++PFG G  +C G +LAKL + I IH + T+Y WS+V   + 
Sbjct: 401 NPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKYSWSIVGASDG 460

Query: 421 IIMDPLPVPYHGMPIKISPKSEI 439
           I   P  +P +G+PI ++ K EI
Sbjct: 461 IQYGPFALPQNGLPIVLARKPEI 465

The following BLAST results are available for this feature:
BLAST of Pay0021410 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q6WG302.3e-8939.55Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2[more]
Q8W4T92.8e-8737.67Taxane 13-alpha-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A2 PE=1 SV=1[more]
Q50EK05.2e-8639.59Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1[more]
Q50EK12.0e-8539.50Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1[more]
Q84KI12.9e-8438.65Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1[more]
BLAST of Pay0021410 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3BLK91.2e-25599.33taxadiene 5-alpha hydroxylase OS=Cucumis melo OX=3656 GN=LOC103491382 PE=3 SV=1[more]
A0A0A0KEB21.0e-24695.28Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G423370 PE=3 SV=1[more]
A0A6J1GL902.4e-21986.07taxadiene 5-alpha hydroxylase isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1GK432.4e-21986.07taxadiene 5-alpha hydroxylase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1GJY92.4e-21986.07taxadiene 5-alpha hydroxylase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
BLAST of Pay0021410 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008449518.12.5e-25599.33PREDICTED: taxadiene 5-alpha hydroxylase [Cucumis melo][more]
XP_004140249.12.2e-24695.28taxadiene 5-alpha hydroxylase [Cucumis sativus] >KAE8647348.1 hypothetical prote... [more]
XP_038888983.17.0e-22989.21taxadiene 5-alpha hydroxylase [Benincasa hispida][more]
KAG6572434.11.1e-22186.97hypothetical protein SDJN03_29162, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7012036.13.1e-22186.74hypothetical protein SDJN02_26944, partial [Cucurbita argyrosperma subsp. argyro... [more]
BLAST of Pay0021410 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G42850.12.5e-16865.75cytochrome P450, family 718 [more]
AT5G36110.11.7e-7938.20cytochrome P450, family 716, subfamily A, polypeptide 1 [more]
AT2G29090.12.3e-6533.79cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
AT2G29090.22.3e-6533.79cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
AT4G19230.14.4e-6431.38cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 185..205
NoneNo IPR availablePANTHERPTHR24286:SF221TAXADIENE 5-ALPHA HYDROXYLASEcoord: 1..434
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 1..434
IPR001128Cytochrome P450PRINTSPR00385P450coord: 304..315
score: 32.64
coord: 375..384
score: 58.89
coord: 248..265
score: 28.61
coord: 384..395
score: 33.52
IPR001128Cytochrome P450PFAMPF00067p450coord: 4..419
e-value: 2.4E-53
score: 181.5
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 374..384
score: 50.41
coord: 343..367
score: 25.98
coord: 384..407
score: 23.19
coord: 30..49
score: 30.76
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..435
e-value: 7.0E-107
score: 359.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 2..434
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 377..386

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0021410.1Pay0021410.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen