Pay0021072 (gene) Melon (Payzawat) v1

BLAST of Pay0021072 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 3.5e-231
Identity = 404/767 (52.67%), Postives = 539/767 (70.27%), Query Frame = 0

Query: 9   LLLFFFYFSLLQTSTIATKKSYIVYLGS-EHSSSLDPSSLSEHSQQVTALHYDLLGSSLG 68
           LLL   + S           SY+VY G+  H   +   ++     +V   HYD LGS  G
Sbjct: 11  LLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAM----DRVKETHYDFLGSFTG 70

Query: 69  SKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGV 128
           S+  A +AIFYSYT+  NGFAA LD   A  ++++P+V+SVF NKA KLHTTRSW+FLG+
Sbjct: 71  SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 130

Query: 129 ENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQT--DSN 188
           E++  +PS+SIW  A+FGED IIAN+DTGVWPESKSF DEG GP+PS+W+GICQ   D+ 
Sbjct: 131 EHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDAT 190

Query: 189 FHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGN 248
           FHCNRKLIG RYF+KGY AA G LN++  + RD DGHG+HTLSTAAG+FV G ++FG GN
Sbjct: 191 FHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGN 250

Query: 249 GTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFN 308
           GTAKGG+P+AR  AYKVCWPP+  ++C+DAD+LAAF+AAI DG DV+S SLGG    +FN
Sbjct: 251 GTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFN 310

Query: 309 DPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKK 368
           D +AI +F A ++ I+VV S GNSGP   TV+NV+PW  TV A+T+DR+F S + LGN K
Sbjct: 311 DSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGK 370

Query: 369 HVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGE 428
           H KG SLSS A    KFYP++ SV+AK  N +   AQ C  G+LDP+K KGKI++C  G+
Sbjct: 371 HYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQ 430

Query: 429 TEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTP 488
              V+KG   +L G  G+++ N    G+++  + H +PA+ +T+ D+  V +Y+  TK P
Sbjct: 431 NGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKP 490

Query: 489 IAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSA 548
           IAH+T  +T L +KPAP++A+FSS+GP+ +   ILKPD+TAPGV+++A+Y   ++PT   
Sbjct: 491 IAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQ 550

Query: 549 ADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILD 608
            D RR+ FN ISGTSMSCPH++GIAGL+K+ +P+WSPAAI+SAIMTTA    +    I +
Sbjct: 551 FDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQN 610

Query: 609 STKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCV 668
           +T +KATP+++GAG V PN A +PGLVYD+ I DYLNFLC+ GY+A +I  F    F C 
Sbjct: 611 ATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCS 670

Query: 669 R-SFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLV 728
                + +LNYPSI+V  L   + +T++R VKNVG P  Y  +V    GV V ++P++L 
Sbjct: 671 SPKISLVNLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLN 730

Query: 729 FRSVGEEKGFKVVL-QNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHL 771
           F  VGE+K FKV+L ++ G V  G VFG L+WSD KH VRSPI V L
Sbjct: 731 FTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Pay0021072 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 3.1e-227
Identity = 416/773 (53.82%), Postives = 527/773 (68.18%), Query Frame = 0

Query: 1   MEASNLSPLLLFFFYFSLLQTSTIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYD 60
           M   +LS LLL     +L  +   A KKSYIVYLGS H+    P   S H   V   H  
Sbjct: 16  MSLQSLSSLLLL---VTLFFSPAFALKKSYIVYLGS-HAHL--PQISSAHLDGVAHSHRT 75

Query: 61  LLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTR 120
            L S +GS   A+EAIFYSY R  NGFAAILD+ EA  +A++P V+SVF NK RKLHTT 
Sbjct: 76  FLASFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTH 135

Query: 121 SWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGIC 180
           SWNF+ +  +  +  +S+WN A +GED IIAN+DTGVWPESKSFSDEGYG VP++W+G C
Sbjct: 136 SWNFMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRC 195

Query: 181 QTDSNFHCNRKLIGGRYFHKGYEA-AGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGAN 240
             D    CNRKLIG RYF+KGY A  G   NA+  T RDHDGHG+HTLSTAAGNFV GAN
Sbjct: 196 HKD--VPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGAN 255

Query: 241 VFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGA 300
           VFG GNGTA GG+PKAR  AYKVCWPP+  ++CFDADILAA EAAI DGVDVLS S+GG 
Sbjct: 256 VFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGD 315

Query: 301 ADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYV 360
           A +Y +D +AI +F AV+ G+ VV S GNSGP   TV+NV+PWV TV A+++DR+F ++V
Sbjct: 316 AGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFV 375

Query: 361 GLGNKKHVKGVSLSSVASLP-KKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKI 420
            L N +  KG SLS    LP +K Y LI++ DA  +N     A  C KG+LDP KVKGKI
Sbjct: 376 ELKNGQSFKGTSLSK--PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKI 435

Query: 421 VICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKY 480
           ++C  G+   VDKG QA+ AGAAG+++ ND   G+EI  + H +PAS I   D + +  Y
Sbjct: 436 LVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSY 495

Query: 481 LKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTG 540
           L STK P  ++ +    L+ KPAP +A+FSSRGPN I   ILKPD+TAPGVNI+A++   
Sbjct: 496 LSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEA 555

Query: 541 IAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGN 600
             PT   +D RR PFN  SGTSMSCPH++G+ GL+K++HP+WSPAAI+SAIMTT++TR N
Sbjct: 556 TGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNN 615

Query: 601 NNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFY 660
             + ++D +  KA P++YG+G V PN AA PGLVYD+T  DYL+FLCA GY+   ++ F 
Sbjct: 616 RRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFA 675

Query: 661 AKP-FNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVT 720
             P + C +   + D NYPSI+V  L     +T+ R++KNVG P TY AR +   GV V+
Sbjct: 676 EDPQYTCRQGANLLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVS 735

Query: 721 IEPSTLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHL 771
           +EP  L F   GE K F++ L+      SG VFG L W+D  H+VRSPI V L
Sbjct: 736 VEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Pay0021072 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 694.5 bits (1791), Expect = 1.4e-198
Identity = 389/785 (49.55%), Postives = 506/785 (64.46%), Query Frame = 0

Query: 9   LLLFFFYFSLLQTSTIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYDLLGSSLGS 68
           +L  FF F+ L  +   +KK YIVY+G+    S  PS  S   +  T  HYDLLGS  GS
Sbjct: 11  ILSSFFLFTFLLAAVNGSKKCYIVYMGAH---SHGPSPTSADLELATDSHYDLLGSIFGS 70

Query: 69  KTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVE 128
           +  A+EAI YSY R  NGFAA+L+++EA ++A+NP V+SVF +K  KLHTTRSW FLG+ 
Sbjct: 71  REKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLH 130

Query: 129 NDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWR-GICQTDS--- 188
                  NS W   +FGE+ II NIDTGVWPES+SFSD+GYG VPSKWR G+CQ +    
Sbjct: 131 RR---GQNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPG 190

Query: 189 --NFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFG 248
                CNRKLIG RY++K +EA  G+L+  L T RD  GHGTHTLSTA GNFV GA VF 
Sbjct: 191 SMKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFA 250

Query: 249 HGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGA--- 308
            GNGTAKGG+P+AR  AYKVCW     + C+ AD+LAA + AI DGVDV++ S G +   
Sbjct: 251 VGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVV 310

Query: 309 -ADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSY 368
            A+  F D ++I AF A+ + IL+V S GN GP P TV NV+PWVFT+AA+T+DRDF S 
Sbjct: 311 TAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSN 370

Query: 369 VGLGNKKHVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKI 428
           + + N+  ++G SL  V   P + + LI S DAK +N T   AQ C +GTLD  KV GKI
Sbjct: 371 LTINNQL-IEGASL-FVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKI 430

Query: 429 VIC-QVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQK 488
           V+C + G+ + V +G +A  AGA G+I+ N ++ G  +  E H     +     A+    
Sbjct: 431 VLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPH 490

Query: 489 YLKST---------KT-PIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAP 548
            +K+T         KT     ++  +TL   KPAP++A+FSSRGPN I  +ILKPDVTAP
Sbjct: 491 GVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAP 550

Query: 549 GVNILASYPTGIAPTFSAADRRR-IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIK 608
           GVNILA+Y    + +    D RR   FNV+ GTSMSCPH +GIAGL+K+ HP+WSPAAIK
Sbjct: 551 GVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIK 610

Query: 609 SAIMTTAKTRGNNNQTILDS-TKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLC 668
           SAIMTTA T  N N+ I D+  K  A  +AYG+G V P+ A +PGLVYD+++ DYLNFLC
Sbjct: 611 SAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLC 670

Query: 669 ARGYDAMKIKKF-YAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTY 728
           A GYD   I    + + F C  S  V DLNYPSI++  L++  P+TI R V NVG P TY
Sbjct: 671 ASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRL-KPVTIARTVTNVGPPSTY 730

Query: 729 VARVKASPGVAVTIEPSTLVFRSVGEEKGFKVVLQ-NTGKVKSGDVFGTLIWSDGKHFVR 769
               ++  G ++ + P +L F  +GE K FKV++Q ++   +    FG L W+DGKH VR
Sbjct: 731 TVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVR 786

BLAST of Pay0021072 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 9.8e-173
Identity = 338/758 (44.59%), Postives = 477/758 (62.93%), Query Frame = 0

Query: 21  TSTIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYDLLGSSLGSKTMAEEAIFYSY 80
           +S+ + + +YIV++    + S  PSS   HS       YD   SSL S + + E + Y+Y
Sbjct: 23  SSSSSDQGTYIVHM----AKSQMPSSFDLHSN-----WYD---SSLRSISDSAE-LLYTY 82

Query: 81  TRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWN 140
             + +GF+  L  +EA++L   P VISV      +LHTTR+  FLG++      +  ++ 
Sbjct: 83  ENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----TADLFP 142

Query: 141 AAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFH---CNRKLIGGRY 200
            A    DV++  +DTGVWPESKS+SDEG+GP+PS W+G C+  +NF    CNRKLIG R+
Sbjct: 143 EAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARF 202

Query: 201 FHKGYEAAGGKLNAT--LLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKA 260
           F +GYE+  G ++ +    + RD DGHGTHT STAAG+ V GA++ G+ +GTA+G AP+A
Sbjct: 203 FARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRA 262

Query: 261 RAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLA 320
           R   YKVCW       CF +DILAA + AIAD V+VLS SLGG   +Y+ D +AI AF A
Sbjct: 263 RVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAA 322

Query: 321 VQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGVSLSSV 380
           ++RGILV  S GN+GP   +++NV+PW+ TV A T+DRDF +   LGN K+  GVSL   
Sbjct: 323 MERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKG 382

Query: 381 ASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGYQA 440
            +LP K  P I + +A  SN T  +   C  GTL P KVKGKIV+C  G    V KG   
Sbjct: 383 EALPDKLLPFIYAGNA--SNAT--NGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVV 442

Query: 441 SLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVKTL 500
             AG  G+I+AN    G+E+  + H +PA+ +      I++ Y+ +   P A ++ + T+
Sbjct: 443 KAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTV 502

Query: 501 LSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPFNV 560
           + VKP+P++A FSSRGPN I   ILKPD+ APGVNILA++     PT  A+D RR+ FN+
Sbjct: 503 VGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNI 562

Query: 561 ISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILD-STKLKATPY 620
           ISGTSMSCPHV+G+A L+KS+HP WSPAAI+SA+MTTA     + + +LD +T   +TP+
Sbjct: 563 ISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPF 622

Query: 621 AYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNC--VRSFKVTD 680
            +GAG V P  A +PGL+YD+T  DYL FLCA  Y + +I+    + + C   +S+ V D
Sbjct: 623 DHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVAD 682

Query: 681 LNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKA-SPGVAVTIEPSTLVFRSVGEE 740
           LNYPS +V    +GA     R V +VG  GTY  +V + + GV +++EP+ L F+   E+
Sbjct: 683 LNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEK 742

Query: 741 KGFKVVLQ-NTGKVKSGDVFGTLIWSDGKHFVRSPIAV 769
           K + V    ++ K    + FG++ WSDGKH V SP+A+
Sbjct: 743 KSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of Pay0021072 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 2.0e-165
Identity = 341/794 (42.95%), Postives = 466/794 (58.69%), Query Frame = 0

Query: 10  LLFFFYFSLLQT-------STIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYDLL 69
           + FFFYF  L T       ++ +   +YIV++  E   S+ P+            H+   
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPT------------HFHWY 60

Query: 70  GSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSW 129
            SSL S T +  +I ++Y   F+GF+A L  ++A  L  +P VISV   + R LHTTRS 
Sbjct: 61  TSSLASLTSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSP 120

Query: 130 NFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQT 189
            FLG+ +        +   + FG D++I  IDTGVWPE  SF D G GPVP KW+G C  
Sbjct: 121 EFLGLRS---TDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIA 180

Query: 190 DSNF---HCNRKLIGGRYFHKGYEAAGGKLNAT--LLTVRDHDGHGTHTLSTAAGNFVTG 249
             +F    CNRKL+G R+F  GYEA  GK+N T    + RD DGHGTHT S +AG +V  
Sbjct: 181 SQDFPESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFP 240

Query: 250 ANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLG 309
           A+  G+ +G A G APKAR  AYKVCW    +S C+D+DILAAF+ A+ADGVDV+S S+G
Sbjct: 241 ASTLGYAHGVAAGMAPKARLAAYKVCW----NSGCYDSDILAAFDTAVADGVDVISLSVG 300

Query: 310 GAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVS 369
           G    Y+ D +AI AF A+ RGI V  S GN GP  +TVTNV+PW+ TV A TIDRDF +
Sbjct: 301 GVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPA 360

Query: 370 YVGLGNKKHVKGVSLSSVASL-PKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKG 429
            V LGN K + GVS+     L P + YPL+            + +  C +G+LDP  VKG
Sbjct: 361 NVKLGNGKMISGVSVYGGPGLDPGRMYPLV--YGGSLLGGDGYSSSLCLEGSLDPNLVKG 420

Query: 430 KIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQ 489
           KIV+C  G      KG      G  G+I+AN +  G+ +  + H +PA+ +  +    ++
Sbjct: 421 KIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIR 480

Query: 490 KYL------KSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVN 549
           +Y+      +S+K P A +    T L ++PAP++A+FS+RGPNP    ILKPDV APG+N
Sbjct: 481 RYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLN 540

Query: 550 ILASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIM 609
           ILA++P  I P+   +D RR  FN++SGTSM+CPHV+G+A L+K+ HP+WSPAAI+SA++
Sbjct: 541 ILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALI 600

Query: 610 TTAKTRGNNNQTILD-STKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGY 669
           TTA T  N+ + ++D ST   ++   YG+G V+P  A DPGLVYDIT  DY+NFLC   Y
Sbjct: 601 TTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNY 660

Query: 670 DAMKIKKFYAKPFNC---VRSFKVTDLNYPSISV-----GELKIGAPLTINRRVKNVG-S 729
               I     +  +C    R+  V +LNYPS SV     GE K+       R V NVG S
Sbjct: 661 TRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFI--RTVTNVGDS 720

Query: 730 PGTYVARVKASPGVAVTIEPSTLVFRSVGEEKGFKVVLQNTG-KVKSGDV---FGTLIWS 771
              Y  +++   G  VT+EP  L FR VG++  F V ++ T  K+  G      G ++WS
Sbjct: 721 DSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWS 771

BLAST of Pay0021072 vs. ExPASy TrEMBL
Match: A0A1S4DX90 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491001 PE=3 SV=1)

HSP 1 Score: 1499.6 bits (3881), Expect = 0.0e+00
Identity = 759/779 (97.43%), Postives = 759/779 (97.43%), Query Frame = 0

Query: 4   SNLSPLLLFFFYFS----LLQTSTI--ATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTAL 63
           SNL   LL F   S     L  STI      SYIVYLGSEHSSSLDPSSLSEHSQQVTAL
Sbjct: 75  SNLKMALLLFESASGLNINLNKSTIFPINVPSYIVYLGSEHSSSLDPSSLSEHSQQVTAL 134

Query: 64  HYDLLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLH 123
           HYDLLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLH
Sbjct: 135 HYDLLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLH 194

Query: 124 TTRSWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWR 183
           TTRSWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWR
Sbjct: 195 TTRSWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWR 254

Query: 184 GICQTDSNFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTG 243
           GICQTDSNFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTG
Sbjct: 255 GICQTDSNFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTG 314

Query: 244 ANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLG 303
           ANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLG
Sbjct: 315 ANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLG 374

Query: 304 GAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVS 363
           GAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVS
Sbjct: 375 GAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVS 434

Query: 364 YVGLGNKKHVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGK 423
           YVGLGNKKHVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGK
Sbjct: 435 YVGLGNKKHVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGK 494

Query: 424 IVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQK 483
           IVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQK
Sbjct: 495 IVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQK 554

Query: 484 YLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPT 543
           YLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPT
Sbjct: 555 YLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPT 614

Query: 544 GIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRG 603
           GIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRG
Sbjct: 615 GIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRG 674

Query: 604 NNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKF 663
           NNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKF
Sbjct: 675 NNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKF 734

Query: 664 YAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVT 723
           YAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVT
Sbjct: 735 YAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVT 794

Query: 724 IEPSTLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 777
           IEPSTLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN
Sbjct: 795 IEPSTLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 853

BLAST of Pay0021072 vs. ExPASy TrEMBL
Match: A0A5D3D763 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00150 PE=3 SV=1)

HSP 1 Score: 1472.2 bits (3810), Expect = 0.0e+00
Identity = 747/784 (95.28%), Postives = 752/784 (95.92%), Query Frame = 0

Query: 1   MEASNLSPLLLFFFYFSLLQTSTIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYD 60
           MEASNLSPLLLFFFYFSLLQTSTIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYD
Sbjct: 1   MEASNLSPLLLFFFYFSLLQTSTIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYD 60

Query: 61  LLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTR 120
           LLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTR
Sbjct: 61  LLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTR 120

Query: 121 SWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGIC 180
           SWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGIC
Sbjct: 121 SWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGIC 180

Query: 181 QTDSNFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANV 240
           QTDSNF CNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANV
Sbjct: 181 QTDSNFLCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANV 240

Query: 241 FGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAA 300
           FGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAA
Sbjct: 241 FGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAA 300

Query: 301 DEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVG 360
           DEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVG
Sbjct: 301 DEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVG 360

Query: 361 LGNKKHVKGVSLSSVASL-PKKFYP-------LINSVDAKFSNVTEFHAQFCGKGTLDPM 420
           LGNKKH+K    + +  L   +F P       L N       +  E   QFCGKGTLDPM
Sbjct: 361 LGNKKHIKVHVYTHLLGLGASRFAPNFICAPELYNQY--SIGSTLETSNQFCGKGTLDPM 420

Query: 421 KVKGKIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDA 480
           KVKGKIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDA
Sbjct: 421 KVKGKIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDA 480

Query: 481 QIVQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNIL 540
           QIVQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNIL
Sbjct: 481 QIVQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNIL 540

Query: 541 ASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTT 600
           ASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTT
Sbjct: 541 ASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTT 600

Query: 601 AKTRGNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAM 660
           AKTRGNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAM
Sbjct: 601 AKTRGNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAM 660

Query: 661 KIKKFYAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASP 720
           KIKKFYAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASP
Sbjct: 661 KIKKFYAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASP 720

Query: 721 GVAVTIEPSTLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGP 777
           GVAVTIEPSTLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGP
Sbjct: 721 GVAVTIEPSTLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGP 780

BLAST of Pay0021072 vs. ExPASy TrEMBL
Match: A0A0A0L241 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045090 PE=3 SV=1)

HSP 1 Score: 1426.8 bits (3692), Expect = 0.0e+00
Identity = 702/750 (93.60%), Postives = 729/750 (97.20%), Query Frame = 0

Query: 28  KSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYDLLGSSLGSKTMAEEAIFYSYTRSFNGF 87
           +SYIVYLGSEHSS+LDPSSLSEHS+QVTALHYDLLGS  GSKTMAEEAIFYSYTRSFNGF
Sbjct: 2   QSYIVYLGSEHSSALDPSSLSEHSRQVTALHYDLLGSLFGSKTMAEEAIFYSYTRSFNGF 61

Query: 88  AAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWNAAKFGED 147
           AA LDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWN AKFGED
Sbjct: 62  AAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWNTAKFGED 121

Query: 148 VIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNRKLIGGRYFHKGYEAAGG 207
           VI+ANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDS FHCNRKLIGGRYFHKGYEAAGG
Sbjct: 122 VIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNRKLIGGRYFHKGYEAAGG 181

Query: 208 KLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKVCWPPL 267
           KLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYK CWPPL
Sbjct: 182 KLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKACWPPL 241

Query: 268 FDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGG 327
           FDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGG
Sbjct: 242 FDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGG 301

Query: 328 NSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGVSLSSVASLPKKFYPLIN 387
           NSGPFPMT+ N+SPWVFTVAA+TIDR+F SYVGLGNKKH+KG+SLSSV SLPKKF+PLIN
Sbjct: 302 NSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGLSLSSVPSLPKKFFPLIN 361

Query: 388 SVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGYQASLAGAAGVIVAN 447
           SVDAKF NVTEFHAQFCGKGTLDPMKVKGKIVICQVGET+GVDKG+QAS AGA GVI+AN
Sbjct: 362 SVDAKFRNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVDKGFQASRAGAVGVIIAN 421

Query: 448 DIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATF 507
           D+EKGDEI+PELHFIPASDITNTDAQIVQ YLKST+TP+AHLTSVKTLLSVKPAP IATF
Sbjct: 422 DLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLTSVKTLLSVKPAPTIATF 481

Query: 508 SSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVA 567
           S+RGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFS  DRRRIPFNVISGTSMSCPHVA
Sbjct: 482 SARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRRIPFNVISGTSMSCPHVA 541

Query: 568 GIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQVYPNDAA 627
           GIAGL+KSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQV PNDAA
Sbjct: 542 GIAGLIKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQVNPNDAA 601

Query: 628 DPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDLNYPSISVGELKIGA 687
           DPGLVYDIT+NDYLNFLCARGY+AM+IKKFYAKPF+CVRSFKVTDLNYPSISVGELKIGA
Sbjct: 602 DPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKVTDLNYPSISVGELKIGA 661

Query: 688 PLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKGFKVVLQNTGKVKSG 747
           PLT+NRRVKNVGSPGTYVARVKASPGVAV+IEPSTLVF  VGEEKGFKVVLQNTGKVK+G
Sbjct: 662 PLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGEEKGFKVVLQNTGKVKNG 721

Query: 748 -DVFGTLIWSDGKHFVRSPIAVHLGPGIGN 777
            DVFGTLIWSDGKHFVRS IAVHLGPGIGN
Sbjct: 722 SDVFGTLIWSDGKHFVRSSIAVHLGPGIGN 751

BLAST of Pay0021072 vs. ExPASy TrEMBL
Match: A0A6J1CQS4 (subtilisin-like protease SBT5.3 OS=Momordica charantia OX=3673 GN=LOC111013337 PE=3 SV=1)

HSP 1 Score: 1102.0 bits (2849), Expect = 0.0e+00
Identity = 549/775 (70.84%), Postives = 648/775 (83.61%), Query Frame = 0

Query: 1   MEASNLSPLLLFFFYFSLLQTSTIA-TKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHY 60
           MEASN+SPLLL FF  SLLQTSTIA  KKSYIVYLGS+  SSL  +SL + S+Q+TA HY
Sbjct: 1   MEASNISPLLLAFFLLSLLQTSTIAIDKKSYIVYLGSQ--SSLASTSLLD-SRQLTASHY 60

Query: 61  DLLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTT 120
           +LLGS LGSK +AEE+IFYSYTRSFNGFAA LD+  A  LA+NP+VISVFEN+ARKLHTT
Sbjct: 61  NLLGSLLGSKKIAEESIFYSYTRSFNGFAATLDENVAARLAKNPQVISVFENRARKLHTT 120

Query: 121 RSWNFLGVENDIGI-PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRG 180
            SWNFLG+EN  G+ P NSIWNAAKFG+D+I+ANIDTGVWPESKSFSDEG+GP+PS+W+G
Sbjct: 121 HSWNFLGMENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSRWKG 180

Query: 181 ICQTDSNFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGA 240
            CQT  NFHCNRKLIGGRYF+KGYEAAG KL+ + LTVRDHDGHGTHTL+TAAGNFV GA
Sbjct: 181 TCQTYLNFHCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGA 240

Query: 241 NVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGG 300
           NVFG GNGTAKGGAP+AR  AYKVCWPPL + +CFDAD LA FEAAIADGVDV+STSLGG
Sbjct: 241 NVFGQGNGTAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGG 300

Query: 301 AADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSY 360
           +  ++ +DPLAIAAF A+Q+GI+ VFS GN GP P +V+NV+PW FTVAA+TIDRDF SY
Sbjct: 301 SVRDFADDPLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASY 360

Query: 361 VGLGNKKHVKGVSLSSVASLPKKFYPLINSVDAKF--SNVTEFHAQFCGKGTLDPMKVKG 420
           + LGNKK +KG SL+SVA LPKKFYPLI+SV  +    NVT++ AQFCG+GT DP KVKG
Sbjct: 361 IALGNKKRIKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKG 420

Query: 421 KIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQ 480
           KI++C  GE  G +KG +A   GAAG+IV ND + GD+I+PELHF+PASD+  +D Q++ 
Sbjct: 421 KILVCLEGEIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLT 480

Query: 481 KYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYP 540
           +Y+ ST+ P+ HL  V+T L +KPAP++A FSSRGPN I+ +ILKPD+TAPGVNILA+Y 
Sbjct: 481 QYMDSTRNPVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYS 540

Query: 541 TGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTR 600
           +G APT S  DRRRIPF+VISGTSMSCPH++GIA L+KSIHP WSPAAIKSAIMTTAKTR
Sbjct: 541 SGNAPTTSRYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTR 600

Query: 601 GNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKK 660
            NN  +ILD TK+KATP+AYGAG V+PNDA DPGLVYD T+ DYLNFLCARGY A +I++
Sbjct: 601 DNNLGSILDLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRR 660

Query: 661 FYAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAV 720
           FYAK + C RSFK+TDLNYPSISV  L +G P+T+NRRVKNVGS GTYVARVK  P V++
Sbjct: 661 FYAKSYVCDRSFKLTDLNYPSISVSNLTMG-PVTVNRRVKNVGSLGTYVARVKVHPEVSI 720

Query: 721 TIEPSTLVFRSVGEEKGFKVVLQNTGKVK-SGDVFGTLIWSDGKHFVRSPIAVHL 771
           ++EPSTL F SVGEEK FKVV Q  GK K  G +FGTLIWSDGKHFVRSPI ++L
Sbjct: 721 SVEPSTLQFSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNL 771

BLAST of Pay0021072 vs. ExPASy TrEMBL
Match: A0A6J1L3L8 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498821 PE=3 SV=1)

HSP 1 Score: 1032.3 bits (2668), Expect = 1.0e-297
Identity = 516/775 (66.58%), Postives = 620/775 (80.00%), Query Frame = 0

Query: 1   MEASNLSPLLLF-FFYFSLLQTS---TIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTA 60
           MEASNL P +LF FF FSLLQTS   TIATKK YIVYL    S S+   S     QQV  
Sbjct: 1   MEASNLIPSILFLFFLFSLLQTSTIATIATKKPYIVYL---ESQSVYADSKHCSIQQVQE 60

Query: 61  LHYDLLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKL 120
            HY+LLGS LGS   A+E+I YSYTRSFNGFAAIL++KEA +LAR+P+VISV EN+ RKL
Sbjct: 61  FHYNLLGSLLGSPEAAKESILYSYTRSFNGFAAILNEKEAADLARSPQVISVLENRGRKL 120

Query: 121 HTTRSWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKW 180
           HTT SW FLGVE+D GIPSNSIWNAA+FG D+IIANIDTGVWPES SFSDEGYGP+PSKW
Sbjct: 121 HTTNSWRFLGVEDDEGIPSNSIWNAAQFGRDMIIANIDTGVWPESNSFSDEGYGPIPSKW 180

Query: 181 RGICQTDSNFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVT 240
           RG C  D +F CNRKLIGGRYF+KGY+ AGG LNAT +++RDH+GHGTHTLSTAAGNFV 
Sbjct: 181 RGTCPDDPSFRCNRKLIGGRYFYKGYKFAGGVLNATSVSIRDHNGHGTHTLSTAAGNFVP 240

Query: 241 GANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSL 300
           GAN+FGHGNGTAKGGAPKAR  AYK CWP + + +CFDAD+LAAFEAAI DGVDV+S SL
Sbjct: 241 GANIFGHGNGTAKGGAPKARVAAYKACWPLIPEGECFDADVLAAFEAAINDGVDVISASL 300

Query: 301 GGAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFV 360
           GG   E+F DP+ IAAF A Q+GI+V+FS GN GP P TV NV+PW  TVAA+T  R+FV
Sbjct: 301 GGGDQEFFRDPVGIAAFHAAQQGIIVIFSAGNDGPQPETVGNVAPWEITVAASTTGRNFV 360

Query: 361 SYVGLGNKKHVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKG 420
           S V LGN K +KG SLSSV++LP +FYPLI+SV+AKFSNV+EF A+FCGKGTL+P KVKG
Sbjct: 361 SNVALGNNKILKGSSLSSVSALP-QFYPLIDSVNAKFSNVSEFQAKFCGKGTLNPKKVKG 420

Query: 421 KIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQ 480
           KI+IC  G+  GV+KGY A+ AGA G+I+A +I+  +EI PEL+F+PAS IT +D +++ 
Sbjct: 421 KILICTAGKITGVEKGYNAAEAGAVGMILATNIDNLEEIKPELYFLPASGITYSDGELLD 480

Query: 481 KYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYP 540
            Y+ ST TP+A + +V+T + + P+P++A FSSRGPNP D  ILKPD+TAPG  ILASYP
Sbjct: 481 DYINSTSTPVAQIMNVRTEVEINPSPVVAAFSSRGPNPFDKAILKPDITAPGFTILASYP 540

Query: 541 TGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTR 600
           T  APT S  D+RR PFNV SGTSM+CPH++ IA L+KSIHP WSPAAIKSA+MTTAKT 
Sbjct: 541 TTRAPTRSGFDQRRTPFNVFSGTSMACPHISAIAALLKSIHPEWSPAAIKSALMTTAKTS 600

Query: 601 GNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKK 660
            NNN        L ATP+A+GAG V PNDA DPGLVYDIT+++YLNFLCARGY+A+++++
Sbjct: 601 DNNN------LLLNATPFAFGAGHVRPNDAMDPGLVYDITVDEYLNFLCARGYNAIQLRR 660

Query: 661 FYAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAV 720
           F  +PF C +SFK  DLNYPSIS+  L + AP+TINRRVKNVG PGTYVARV+   GVA 
Sbjct: 661 FSKQPFVCEKSFKAIDLNYPSISIPNLNVNAPVTINRRVKNVGGPGTYVARVELPEGVAA 720

Query: 721 TIEPSTLVFRSVGEEKGFKVVLQNTGKVK-SGDVFGTLIWSDGKHFVRSPIAVHL 771
           ++EPSTL F SVGEEK F+VV+QNTG++K  G VFG L+WSDGKH V SPI+++L
Sbjct: 721 SVEPSTLQFSSVGEEKAFRVVVQNTGELKHEGYVFGKLVWSDGKHSVGSPISMNL 765

BLAST of Pay0021072 vs. NCBI nr
Match: XP_016900593.1 (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo])

HSP 1 Score: 1499.6 bits (3881), Expect = 0.0e+00
Identity = 759/779 (97.43%), Postives = 759/779 (97.43%), Query Frame = 0

Query: 4   SNLSPLLLFFFYFS----LLQTSTI--ATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTAL 63
           SNL   LL F   S     L  STI      SYIVYLGSEHSSSLDPSSLSEHSQQVTAL
Sbjct: 75  SNLKMALLLFESASGLNINLNKSTIFPINVPSYIVYLGSEHSSSLDPSSLSEHSQQVTAL 134

Query: 64  HYDLLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLH 123
           HYDLLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLH
Sbjct: 135 HYDLLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLH 194

Query: 124 TTRSWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWR 183
           TTRSWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWR
Sbjct: 195 TTRSWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWR 254

Query: 184 GICQTDSNFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTG 243
           GICQTDSNFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTG
Sbjct: 255 GICQTDSNFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTG 314

Query: 244 ANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLG 303
           ANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLG
Sbjct: 315 ANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLG 374

Query: 304 GAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVS 363
           GAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVS
Sbjct: 375 GAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVS 434

Query: 364 YVGLGNKKHVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGK 423
           YVGLGNKKHVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGK
Sbjct: 435 YVGLGNKKHVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGK 494

Query: 424 IVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQK 483
           IVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQK
Sbjct: 495 IVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQK 554

Query: 484 YLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPT 543
           YLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPT
Sbjct: 555 YLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPT 614

Query: 544 GIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRG 603
           GIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRG
Sbjct: 615 GIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRG 674

Query: 604 NNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKF 663
           NNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKF
Sbjct: 675 NNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKF 734

Query: 664 YAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVT 723
           YAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVT
Sbjct: 735 YAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVT 794

Query: 724 IEPSTLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 777
           IEPSTLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN
Sbjct: 795 IEPSTLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 853

BLAST of Pay0021072 vs. NCBI nr
Match: TYK19384.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])

HSP 1 Score: 1472.2 bits (3810), Expect = 0.0e+00
Identity = 747/784 (95.28%), Postives = 752/784 (95.92%), Query Frame = 0

Query: 1   MEASNLSPLLLFFFYFSLLQTSTIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYD 60
           MEASNLSPLLLFFFYFSLLQTSTIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYD
Sbjct: 1   MEASNLSPLLLFFFYFSLLQTSTIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYD 60

Query: 61  LLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTR 120
           LLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTR
Sbjct: 61  LLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTR 120

Query: 121 SWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGIC 180
           SWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGIC
Sbjct: 121 SWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGIC 180

Query: 181 QTDSNFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANV 240
           QTDSNF CNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANV
Sbjct: 181 QTDSNFLCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANV 240

Query: 241 FGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAA 300
           FGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAA
Sbjct: 241 FGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAA 300

Query: 301 DEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVG 360
           DEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVG
Sbjct: 301 DEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVG 360

Query: 361 LGNKKHVKGVSLSSVASL-PKKFYP-------LINSVDAKFSNVTEFHAQFCGKGTLDPM 420
           LGNKKH+K    + +  L   +F P       L N       +  E   QFCGKGTLDPM
Sbjct: 361 LGNKKHIKVHVYTHLLGLGASRFAPNFICAPELYNQY--SIGSTLETSNQFCGKGTLDPM 420

Query: 421 KVKGKIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDA 480
           KVKGKIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDA
Sbjct: 421 KVKGKIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDA 480

Query: 481 QIVQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNIL 540
           QIVQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNIL
Sbjct: 481 QIVQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNIL 540

Query: 541 ASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTT 600
           ASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTT
Sbjct: 541 ASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTT 600

Query: 601 AKTRGNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAM 660
           AKTRGNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAM
Sbjct: 601 AKTRGNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAM 660

Query: 661 KIKKFYAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASP 720
           KIKKFYAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASP
Sbjct: 661 KIKKFYAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASP 720

Query: 721 GVAVTIEPSTLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGP 777
           GVAVTIEPSTLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGP
Sbjct: 721 GVAVTIEPSTLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGP 780

BLAST of Pay0021072 vs. NCBI nr
Match: XP_031738719.1 (uncharacterized protein LOC101212014 [Cucumis sativus])

HSP 1 Score: 1426.0 bits (3690), Expect = 0.0e+00
Identity = 702/749 (93.72%), Postives = 728/749 (97.20%), Query Frame = 0

Query: 29   SYIVYLGSEHSSSLDPSSLSEHSQQVTALHYDLLGSSLGSKTMAEEAIFYSYTRSFNGFA 88
            SYIVYLGSEHSS+LDPSSLSEHS+QVTALHYDLLGS  GSKTMAEEAIFYSYTRSFNGFA
Sbjct: 796  SYIVYLGSEHSSALDPSSLSEHSRQVTALHYDLLGSLFGSKTMAEEAIFYSYTRSFNGFA 855

Query: 89   AILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWNAAKFGEDV 148
            A LDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWN AKFGEDV
Sbjct: 856  AKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWNTAKFGEDV 915

Query: 149  IIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNRKLIGGRYFHKGYEAAGGK 208
            I+ANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDS FHCNRKLIGGRYFHKGYEAAGGK
Sbjct: 916  IVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNRKLIGGRYFHKGYEAAGGK 975

Query: 209  LNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKVCWPPLF 268
            LNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYK CWPPLF
Sbjct: 976  LNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKACWPPLF 1035

Query: 269  DSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGGN 328
            DSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGGN
Sbjct: 1036 DSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGGN 1095

Query: 329  SGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGVSLSSVASLPKKFYPLINS 388
            SGPFPMT+ N+SPWVFTVAA+TIDR+F SYVGLGNKKH+KG+SLSSV SLPKKF+PLINS
Sbjct: 1096 SGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGLSLSSVPSLPKKFFPLINS 1155

Query: 389  VDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGYQASLAGAAGVIVAND 448
            VDAKF NVTEFHAQFCGKGTLDPMKVKGKIVICQVGET+GVDKG+QAS AGA GVI+AND
Sbjct: 1156 VDAKFRNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVDKGFQASRAGAVGVIIAND 1215

Query: 449  IEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFS 508
            +EKGDEI+PELHFIPASDITNTDAQIVQ YLKST+TP+AHLTSVKTLLSVKPAP IATFS
Sbjct: 1216 LEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLTSVKTLLSVKPAPTIATFS 1275

Query: 509  SRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAG 568
            +RGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFS  DRRRIPFNVISGTSMSCPHVAG
Sbjct: 1276 ARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRRIPFNVISGTSMSCPHVAG 1335

Query: 569  IAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQVYPNDAAD 628
            IAGL+KSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQV PNDAAD
Sbjct: 1336 IAGLIKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQVNPNDAAD 1395

Query: 629  PGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDLNYPSISVGELKIGAP 688
            PGLVYDIT+NDYLNFLCARGY+AM+IKKFYAKPF+CVRSFKVTDLNYPSISVGELKIGAP
Sbjct: 1396 PGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKVTDLNYPSISVGELKIGAP 1455

Query: 689  LTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKGFKVVLQNTGKVKSG- 748
            LT+NRRVKNVGSPGTYVARVKASPGVAV+IEPSTLVF  VGEEKGFKVVLQNTGKVK+G 
Sbjct: 1456 LTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGEEKGFKVVLQNTGKVKNGS 1515

Query: 749  DVFGTLIWSDGKHFVRSPIAVHLGPGIGN 777
            DVFGTLIWSDGKHFVRS IAVHLGPGIGN
Sbjct: 1516 DVFGTLIWSDGKHFVRSSIAVHLGPGIGN 1544

BLAST of Pay0021072 vs. NCBI nr
Match: KAE8650095.1 (hypothetical protein Csa_011146 [Cucumis sativus])

HSP 1 Score: 1392.1 bits (3602), Expect = 0.0e+00
Identity = 688/741 (92.85%), Postives = 715/741 (96.49%), Query Frame = 0

Query: 28  KSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYDLLGSSLGSKTMAEEAIFYSYTRSFNGF 87
           +SYIVYLGSEHSS+LDPSSLSEHS+QVTALHYDLLGS  GSKTMAEEAIFYSYTRSFNGF
Sbjct: 2   QSYIVYLGSEHSSALDPSSLSEHSRQVTALHYDLLGSLFGSKTMAEEAIFYSYTRSFNGF 61

Query: 88  AAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWNAAKFGED 147
           AA LDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWN AKFGED
Sbjct: 62  AAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWNTAKFGED 121

Query: 148 VIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNRKLIGGRYFHKGYEAAGG 207
           VI+ANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDS FHCNRKLIGGRYFHKGYEAAGG
Sbjct: 122 VIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNRKLIGGRYFHKGYEAAGG 181

Query: 208 KLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKVCWPPL 267
           KLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYK CWPPL
Sbjct: 182 KLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKACWPPL 241

Query: 268 FDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGG 327
           FDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGG
Sbjct: 242 FDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGG 301

Query: 328 NSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGVSLSSVASLPKKFYPLIN 387
           NSGPFPMT+ N+SPWVFTVAA+TIDR+F SYVGLGNKKH+KG+SLSSV SLPKKF+PLIN
Sbjct: 302 NSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGLSLSSVPSLPKKFFPLIN 361

Query: 388 SVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGYQASLAGAAGVIVAN 447
           SVDAKF NVTEFHAQFCGKGTLDPMKVKGKIVICQVGET+GVDKG+QAS AGA GVI+AN
Sbjct: 362 SVDAKFRNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVDKGFQASRAGAVGVIIAN 421

Query: 448 DIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATF 507
           D+EKGDEI+PELHFIPASDITNTDAQIVQ YLKST+TP+AHLTSVKTLLSVKPAP IATF
Sbjct: 422 DLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLTSVKTLLSVKPAPTIATF 481

Query: 508 SSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVA 567
           S+RGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFS  DRRRIPFNVISGTSMSCPHVA
Sbjct: 482 SARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRRIPFNVISGTSMSCPHVA 541

Query: 568 GIAGLVKSIHPNWSPAAIKSAIMTT------AKTRGNNNQTILDSTKLKATPYAYGAGQV 627
           GIAGL+KSIHPNWSPAAIKSAIMTT      AKTRGNNNQTILDSTKLKATPYAYGAGQV
Sbjct: 542 GIAGLIKSIHPNWSPAAIKSAIMTTVTYSFAAKTRGNNNQTILDSTKLKATPYAYGAGQV 601

Query: 628 YPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDLNYPSISVG 687
            PNDAADPGLVYDIT+NDYLNFLCARGY+AM+IKKFYAKPF+CVRSFKVTDLNYPSISVG
Sbjct: 602 NPNDAADPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKVTDLNYPSISVG 661

Query: 688 ELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKGFKVVLQNT 747
           ELKIGAPLT+NRRVKNVGSPGTYVARVKASPGVAV+IEPSTLVF  VGEEKGFKVVLQNT
Sbjct: 662 ELKIGAPLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGEEKGFKVVLQNT 721

Query: 748 GKVKSG-DVFGTLIWSDGKHF 762
           GKVK+G DVFGTLIWSDGKHF
Sbjct: 722 GKVKNGSDVFGTLIWSDGKHF 742

BLAST of Pay0021072 vs. NCBI nr
Match: XP_038904203.1 (uncharacterized protein LOC120090550 [Benincasa hispida])

HSP 1 Score: 1354.7 bits (3505), Expect = 0.0e+00
Identity = 669/773 (86.55%), Postives = 715/773 (92.50%), Query Frame = 0

Query: 1   MEASNLSPLLLFFFYFSLLQTSTIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYD 60
           MEASNLSPLLLFFFYFSLLQTS IATKKSYIVYLGSE SSSLDPSSLSEHS+QVTA HYD
Sbjct: 1   MEASNLSPLLLFFFYFSLLQTSIIATKKSYIVYLGSERSSSLDPSSLSEHSRQVTAFHYD 60

Query: 61  LLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTR 120
           LLG+ LGS+ M EEAIFYSYT SFNGFAA LD+KEA NLARNPKVISVFENKARKLHTTR
Sbjct: 61  LLGTLLGSQKMTEEAIFYSYTTSFNGFAAKLDEKEAANLARNPKVISVFENKARKLHTTR 120

Query: 121 SWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGIC 180
           SWNFLGVEND+GIPSNSIWNAAKFG+D+IIANIDTGVWPESKSFSDEGYGPVPSKWRGIC
Sbjct: 121 SWNFLGVENDVGIPSNSIWNAAKFGQDIIIANIDTGVWPESKSFSDEGYGPVPSKWRGIC 180

Query: 181 QTDSNFHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANV 240
           QTDSNFHCNRKLIGGRYF+KGY AAGG LNAT LTVRDHDGHGTHTLSTAAGNFVTGANV
Sbjct: 181 QTDSNFHCNRKLIGGRYFYKGYVAAGGTLNATSLTVRDHDGHGTHTLSTAAGNFVTGANV 240

Query: 241 FGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAA 300
           FGHG+GTAKGGAPKAR  AYKVCWPP   SQC DADILAAFEAA+ADGVDV+STSLGGAA
Sbjct: 241 FGHGSGTAKGGAPKARVAAYKVCWPPFLGSQCLDADILAAFEAAVADGVDVISTSLGGAA 300

Query: 301 DEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVG 360
           DEYFNDPLAIAAF AVQ+G++VVFS GNSGPFPMTVTN++PW+ TVAA T+DRDF S V 
Sbjct: 301 DEYFNDPLAIAAFHAVQQGVVVVFSAGNSGPFPMTVTNIAPWINTVAAGTVDRDFASNVA 360

Query: 361 LGNKKHVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVI 420
           LGNK   +GVSLSS+A LPKKFYPLI+SV+AK SNVTEFHA+FCG+GTLDPMKVKGKIVI
Sbjct: 361 LGNK---EGVSLSSIAPLPKKFYPLIDSVNAKLSNVTEFHARFCGEGTLDPMKVKGKIVI 420

Query: 421 CQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLK 480
           CQVGE EGV+K YQA+ AGA GVIVANDIEKGDEIYPELHFIPASDITN DAQ++QKYL 
Sbjct: 421 CQVGEIEGVEKSYQAARAGAVGVIVANDIEKGDEIYPELHFIPASDITNNDAQLLQKYLN 480

Query: 481 STKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIA 540
           ST TP+AHLT VKTLL++KPAPIIATFSSRGPNPID  ILKPD+TAPGVNILASY TG A
Sbjct: 481 STTTPMAHLTKVKTLLNIKPAPIIATFSSRGPNPIDPFILKPDITAPGVNILASYSTGNA 540

Query: 541 PTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNN 600
           PTFS+ D+RRIPFNVISGTSMSCPH+AGIAGL+KSIHP+WSPAAIKSAIMTTAKTRGNN 
Sbjct: 541 PTFSSNDQRRIPFNVISGTSMSCPHIAGIAGLLKSIHPDWSPAAIKSAIMTTAKTRGNNL 600

Query: 601 QTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAK 660
           QTILDSTKLKAT YAYGAG V+PNDA DPGLVYD TI DYLNFLCARGY+AM++KKFYAK
Sbjct: 601 QTILDSTKLKATSYAYGAGMVHPNDARDPGLVYDTTIEDYLNFLCARGYNAMEMKKFYAK 660

Query: 661 PFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEP 720
           PFNCV+SFK TDLNYPSISVG L+IGAP+TINRRVK+VGSPGTYVARVK SPGVAV +EP
Sbjct: 661 PFNCVKSFKATDLNYPSISVGVLRIGAPVTINRRVKSVGSPGTYVARVKVSPGVAVLVEP 720

Query: 721 STLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPG 774
            TL F SVGEEK FKVVLQNTGKVK GDVFGTLIWSDGKHFVRSPIAVHLGPG
Sbjct: 721 RTLQFSSVGEEKAFKVVLQNTGKVKRGDVFGTLIWSDGKHFVRSPIAVHLGPG 770

BLAST of Pay0021072 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 802.7 bits (2072), Expect = 2.5e-232
Identity = 404/767 (52.67%), Postives = 539/767 (70.27%), Query Frame = 0

Query: 9   LLLFFFYFSLLQTSTIATKKSYIVYLGS-EHSSSLDPSSLSEHSQQVTALHYDLLGSSLG 68
           LLL   + S           SY+VY G+  H   +   ++     +V   HYD LGS  G
Sbjct: 11  LLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAM----DRVKETHYDFLGSFTG 70

Query: 69  SKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGV 128
           S+  A +AIFYSYT+  NGFAA LD   A  ++++P+V+SVF NKA KLHTTRSW+FLG+
Sbjct: 71  SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 130

Query: 129 ENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQT--DSN 188
           E++  +PS+SIW  A+FGED IIAN+DTGVWPESKSF DEG GP+PS+W+GICQ   D+ 
Sbjct: 131 EHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDAT 190

Query: 189 FHCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGN 248
           FHCNRKLIG RYF+KGY AA G LN++  + RD DGHG+HTLSTAAG+FV G ++FG GN
Sbjct: 191 FHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGN 250

Query: 249 GTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFN 308
           GTAKGG+P+AR  AYKVCWPP+  ++C+DAD+LAAF+AAI DG DV+S SLGG    +FN
Sbjct: 251 GTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFN 310

Query: 309 DPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKK 368
           D +AI +F A ++ I+VV S GNSGP   TV+NV+PW  TV A+T+DR+F S + LGN K
Sbjct: 311 DSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGK 370

Query: 369 HVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGE 428
           H KG SLSS A    KFYP++ SV+AK  N +   AQ C  G+LDP+K KGKI++C  G+
Sbjct: 371 HYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQ 430

Query: 429 TEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTP 488
              V+KG   +L G  G+++ N    G+++  + H +PA+ +T+ D+  V +Y+  TK P
Sbjct: 431 NGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKP 490

Query: 489 IAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSA 548
           IAH+T  +T L +KPAP++A+FSS+GP+ +   ILKPD+TAPGV+++A+Y   ++PT   
Sbjct: 491 IAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQ 550

Query: 549 ADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILD 608
            D RR+ FN ISGTSMSCPH++GIAGL+K+ +P+WSPAAI+SAIMTTA    +    I +
Sbjct: 551 FDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQN 610

Query: 609 STKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCV 668
           +T +KATP+++GAG V PN A +PGLVYD+ I DYLNFLC+ GY+A +I  F    F C 
Sbjct: 611 ATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCS 670

Query: 669 R-SFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLV 728
                + +LNYPSI+V  L   + +T++R VKNVG P  Y  +V    GV V ++P++L 
Sbjct: 671 SPKISLVNLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLN 730

Query: 729 FRSVGEEKGFKVVL-QNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHL 771
           F  VGE+K FKV+L ++ G V  G VFG L+WSD KH VRSPI V L
Sbjct: 731 FTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Pay0021072 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 789.6 bits (2038), Expect = 2.2e-228
Identity = 416/773 (53.82%), Postives = 527/773 (68.18%), Query Frame = 0

Query: 1   MEASNLSPLLLFFFYFSLLQTSTIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYD 60
           M   +LS LLL     +L  +   A KKSYIVYLGS H+    P   S H   V   H  
Sbjct: 16  MSLQSLSSLLLL---VTLFFSPAFALKKSYIVYLGS-HAHL--PQISSAHLDGVAHSHRT 75

Query: 61  LLGSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTR 120
            L S +GS   A+EAIFYSY R  NGFAAILD+ EA  +A++P V+SVF NK RKLHTT 
Sbjct: 76  FLASFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTH 135

Query: 121 SWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGIC 180
           SWNF+ +  +  +  +S+WN A +GED IIAN+DTGVWPESKSFSDEGYG VP++W+G C
Sbjct: 136 SWNFMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRC 195

Query: 181 QTDSNFHCNRKLIGGRYFHKGYEA-AGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGAN 240
             D    CNRKLIG RYF+KGY A  G   NA+  T RDHDGHG+HTLSTAAGNFV GAN
Sbjct: 196 HKD--VPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGAN 255

Query: 241 VFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGA 300
           VFG GNGTA GG+PKAR  AYKVCWPP+  ++CFDADILAA EAAI DGVDVLS S+GG 
Sbjct: 256 VFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGD 315

Query: 301 ADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYV 360
           A +Y +D +AI +F AV+ G+ VV S GNSGP   TV+NV+PWV TV A+++DR+F ++V
Sbjct: 316 AGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFV 375

Query: 361 GLGNKKHVKGVSLSSVASLP-KKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKI 420
            L N +  KG SLS    LP +K Y LI++ DA  +N     A  C KG+LDP KVKGKI
Sbjct: 376 ELKNGQSFKGTSLSK--PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKI 435

Query: 421 VICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKY 480
           ++C  G+   VDKG QA+ AGAAG+++ ND   G+EI  + H +PAS I   D + +  Y
Sbjct: 436 LVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSY 495

Query: 481 LKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTG 540
           L STK P  ++ +    L+ KPAP +A+FSSRGPN I   ILKPD+TAPGVNI+A++   
Sbjct: 496 LSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEA 555

Query: 541 IAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGN 600
             PT   +D RR PFN  SGTSMSCPH++G+ GL+K++HP+WSPAAI+SAIMTT++TR N
Sbjct: 556 TGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNN 615

Query: 601 NNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFY 660
             + ++D +  KA P++YG+G V PN AA PGLVYD+T  DYL+FLCA GY+   ++ F 
Sbjct: 616 RRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFA 675

Query: 661 AKP-FNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVT 720
             P + C +   + D NYPSI+V  L     +T+ R++KNVG P TY AR +   GV V+
Sbjct: 676 EDPQYTCRQGANLLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVS 735

Query: 721 IEPSTLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHL 771
           +EP  L F   GE K F++ L+      SG VFG L W+D  H+VRSPI V L
Sbjct: 736 VEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Pay0021072 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 608.6 bits (1568), Expect = 7.0e-174
Identity = 338/758 (44.59%), Postives = 477/758 (62.93%), Query Frame = 0

Query: 21  TSTIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYDLLGSSLGSKTMAEEAIFYSY 80
           +S+ + + +YIV++    + S  PSS   HS       YD   SSL S + + E + Y+Y
Sbjct: 23  SSSSSDQGTYIVHM----AKSQMPSSFDLHSN-----WYD---SSLRSISDSAE-LLYTY 82

Query: 81  TRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWN 140
             + +GF+  L  +EA++L   P VISV      +LHTTR+  FLG++      +  ++ 
Sbjct: 83  ENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----TADLFP 142

Query: 141 AAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFH---CNRKLIGGRY 200
            A    DV++  +DTGVWPESKS+SDEG+GP+PS W+G C+  +NF    CNRKLIG R+
Sbjct: 143 EAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARF 202

Query: 201 FHKGYEAAGGKLNAT--LLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKA 260
           F +GYE+  G ++ +    + RD DGHGTHT STAAG+ V GA++ G+ +GTA+G AP+A
Sbjct: 203 FARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRA 262

Query: 261 RAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLA 320
           R   YKVCW       CF +DILAA + AIAD V+VLS SLGG   +Y+ D +AI AF A
Sbjct: 263 RVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAA 322

Query: 321 VQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGVSLSSV 380
           ++RGILV  S GN+GP   +++NV+PW+ TV A T+DRDF +   LGN K+  GVSL   
Sbjct: 323 MERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKG 382

Query: 381 ASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGYQA 440
            +LP K  P I + +A  SN T  +   C  GTL P KVKGKIV+C  G    V KG   
Sbjct: 383 EALPDKLLPFIYAGNA--SNAT--NGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVV 442

Query: 441 SLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVKTL 500
             AG  G+I+AN    G+E+  + H +PA+ +      I++ Y+ +   P A ++ + T+
Sbjct: 443 KAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTV 502

Query: 501 LSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPFNV 560
           + VKP+P++A FSSRGPN I   ILKPD+ APGVNILA++     PT  A+D RR+ FN+
Sbjct: 503 VGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNI 562

Query: 561 ISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILD-STKLKATPY 620
           ISGTSMSCPHV+G+A L+KS+HP WSPAAI+SA+MTTA     + + +LD +T   +TP+
Sbjct: 563 ISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPF 622

Query: 621 AYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNC--VRSFKVTD 680
            +GAG V P  A +PGL+YD+T  DYL FLCA  Y + +I+    + + C   +S+ V D
Sbjct: 623 DHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVAD 682

Query: 681 LNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKA-SPGVAVTIEPSTLVFRSVGEE 740
           LNYPS +V    +GA     R V +VG  GTY  +V + + GV +++EP+ L F+   E+
Sbjct: 683 LNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEK 742

Query: 741 KGFKVVLQ-NTGKVKSGDVFGTLIWSDGKHFVRSPIAV 769
           K + V    ++ K    + FG++ WSDGKH V SP+A+
Sbjct: 743 KSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of Pay0021072 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 584.3 bits (1505), Expect = 1.4e-166
Identity = 341/794 (42.95%), Postives = 466/794 (58.69%), Query Frame = 0

Query: 10  LLFFFYFSLLQT-------STIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYDLL 69
           + FFFYF  L T       ++ +   +YIV++  E   S+ P+            H+   
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPT------------HFHWY 60

Query: 70  GSSLGSKTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSW 129
            SSL S T +  +I ++Y   F+GF+A L  ++A  L  +P VISV   + R LHTTRS 
Sbjct: 61  TSSLASLTSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSP 120

Query: 130 NFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQT 189
            FLG+ +        +   + FG D++I  IDTGVWPE  SF D G GPVP KW+G C  
Sbjct: 121 EFLGLRS---TDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIA 180

Query: 190 DSNF---HCNRKLIGGRYFHKGYEAAGGKLNAT--LLTVRDHDGHGTHTLSTAAGNFVTG 249
             +F    CNRKL+G R+F  GYEA  GK+N T    + RD DGHGTHT S +AG +V  
Sbjct: 181 SQDFPESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFP 240

Query: 250 ANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLG 309
           A+  G+ +G A G APKAR  AYKVCW    +S C+D+DILAAF+ A+ADGVDV+S S+G
Sbjct: 241 ASTLGYAHGVAAGMAPKARLAAYKVCW----NSGCYDSDILAAFDTAVADGVDVISLSVG 300

Query: 310 GAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVS 369
           G    Y+ D +AI AF A+ RGI V  S GN GP  +TVTNV+PW+ TV A TIDRDF +
Sbjct: 301 GVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPA 360

Query: 370 YVGLGNKKHVKGVSLSSVASL-PKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKG 429
            V LGN K + GVS+     L P + YPL+            + +  C +G+LDP  VKG
Sbjct: 361 NVKLGNGKMISGVSVYGGPGLDPGRMYPLV--YGGSLLGGDGYSSSLCLEGSLDPNLVKG 420

Query: 430 KIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQ 489
           KIV+C  G      KG      G  G+I+AN +  G+ +  + H +PA+ +  +    ++
Sbjct: 421 KIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIR 480

Query: 490 KYL------KSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVN 549
           +Y+      +S+K P A +    T L ++PAP++A+FS+RGPNP    ILKPDV APG+N
Sbjct: 481 RYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLN 540

Query: 550 ILASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIM 609
           ILA++P  I P+   +D RR  FN++SGTSM+CPHV+G+A L+K+ HP+WSPAAI+SA++
Sbjct: 541 ILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALI 600

Query: 610 TTAKTRGNNNQTILD-STKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGY 669
           TTA T  N+ + ++D ST   ++   YG+G V+P  A DPGLVYDIT  DY+NFLC   Y
Sbjct: 601 TTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNY 660

Query: 670 DAMKIKKFYAKPFNC---VRSFKVTDLNYPSISV-----GELKIGAPLTINRRVKNVG-S 729
               I     +  +C    R+  V +LNYPS SV     GE K+       R V NVG S
Sbjct: 661 TRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFI--RTVTNVGDS 720

Query: 730 PGTYVARVKASPGVAVTIEPSTLVFRSVGEEKGFKVVLQNTG-KVKSGDV---FGTLIWS 771
              Y  +++   G  VT+EP  L FR VG++  F V ++ T  K+  G      G ++WS
Sbjct: 721 DSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWS 771

BLAST of Pay0021072 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 583.9 bits (1504), Expect = 1.8e-166
Identity = 334/781 (42.77%), Postives = 472/781 (60.44%), Query Frame = 0

Query: 9   LLLFFFYFSLLQTSTIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYDLLGSSLGS 68
           +LL F  F  +  +     K++I  +      S+ P+            HY    +    
Sbjct: 7   VLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPT------------HYHWYSTEFAE 66

Query: 69  KTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVE 128
               E  I + Y   F+GF+A++   EA+NL  +P V++VFE++ R+LHTTRS  FLG++
Sbjct: 67  ----ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ 126

Query: 129 NDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNF-- 188
           N  G     +W+ + +G DVII   DTG+WPE +SFSD   GP+P +WRG+C++ + F  
Sbjct: 127 NQKG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSP 186

Query: 189 -HCNRKLIGGRYFHKGYEAAG-GKLNATL--LTVRDHDGHGTHTLSTAAGNFVTGANVFG 248
            +CNRK+IG R+F KG +AA  G +N T+  L+ RD DGHGTHT STAAG     A++ G
Sbjct: 187 RNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSG 246

Query: 249 HGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGA--- 308
           + +G AKG APKAR  AYKVCW    DS C D+DILAAF+AA+ DGVDV+S S+GG    
Sbjct: 247 YASGVAKGVAPKARIAAYKVCWK---DSGCLDSDILAAFDAAVRDGVDVISISIGGGDGI 306

Query: 309 ADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYV 368
              Y+ DP+AI ++ A  +GI V  S GN GP  M+VTN++PWV TV A+TIDR+F +  
Sbjct: 307 TSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADA 366

Query: 369 GLGNKKHVKGVSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIV 428
            LG+   ++GVSL +   L  + +P++    +  S+     A  C + TLDP +V+GKIV
Sbjct: 367 ILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSS-----ASLCMENTLDPKQVRGKIV 426

Query: 429 ICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYL 488
           IC  G +  V KG     AG  G+I+AN    G+ +  + H IPA  + + +   ++ Y 
Sbjct: 427 ICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYA 486

Query: 489 KSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGI 548
            S   PIA +    T++ +KPAP+IA+FS RGPN +   ILKPD+ APGVNILA++   +
Sbjct: 487 SSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAV 546

Query: 549 APTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNN 608
            PT   +D R+  FN++SGTSM+CPHV+G A L+KS HP+WSPA I+SA+MTT     N+
Sbjct: 547 GPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNS 606

Query: 609 NQTILD-STKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFY 668
           N++++D ST   ATPY YG+G +    A +PGLVYDIT +DY+ FLC+ GY    I+   
Sbjct: 607 NRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVIT 666

Query: 669 AKPFNCVRSFKVT--DLNYPSISV---GELKIGAPLTINRRVKNVG-SPGTYVARVKASP 728
             P  C  + K +  +LNYPSI+       +     T+ R   NVG +   Y AR+++  
Sbjct: 667 RTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPR 726

Query: 729 GVAVTIEPSTLVFRSVGEEKGFKV-VLQNTGKV---KSGDVFGTLIWSD-GKHFVRSPIA 769
           GV VT++P  LVF S  + + + V V  NT  V   ++G VFG++ W D GKH VRSPI 
Sbjct: 727 GVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIV 758