Pay0020929 (gene) Melon (Payzawat) v1

Overview
NamePay0020929
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionCalcium-binding EF hand family protein
Locationchr04: 27818521 .. 27819217 (+)
RNA-Seq ExpressionPay0020929
SyntenyPay0020929
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCTCCAATTCACCACATAACTCAGTCCCCAAACAAAATTCTATATCCATGTTCTTTAGATTAGAGTACCATTTAATTACAATGCAAACGCAGGTAACCAACGCTGTCAAGAGCGTGCAGCTCGACCCGTCACCGCTCGTTTTTGTCGATTGGATATCAAATCTACCAATTTTATTCAACTTTGCAGGCAGAATACATGAGCTGATTAGATCTCTACCTTATTGGTCAATTCTTCAATCACAGTACTCCAAAGAGAAAGCTTTATCACAAAGTAATTGCTCTTCACAATCTGATGATAAAATCATCATCACTACTCAACAAAATTGCTCAATGAGCAGAGAGGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGGAGGGAAGAAAATGATGATGATGATGATGATGAAGAAATTGGGGCAAGTGATGAAATTATAAGAGGTATGTTTGAAGAGAATGAACCAAGTTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAAAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCATGATTGCAAAACAATGATAGCCAGATTTGATCTCAATAATGATGGTAAAATTGATTTCCATGAATTTGTTAAGTTTATGGAAGTTGCTTTGTGTTAGAATATTTTGC

mRNA sequence

ATCTCCAATTCACCACATAACTCAGTCCCCAAACAAAATTCTATATCCATGTTCTTTAGATTAGAGTACCATTTAATTACAATGCAAACGCAGGTAACCAACGCTGTCAAGAGCGTGCAGCTCGACCCGTCACCGCTCGTTTTTGTCGATTGGATATCAAATCTACCAATTTTATTCAACTTTGCAGGCAGAATACATGAGCTGATTAGATCTCTACCTTATTGGTCAATTCTTCAATCACAGTACTCCAAAGAGAAAGCTTTATCACAAAGTAATTGCTCTTCACAATCTGATGATAAAATCATCATCACTACTCAACAAAATTGCTCAATGAGCAGAGAGGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGGAGGGAAGAAAATGATGATGATGATGATGATGAAGAAATTGGGGCAAGTGATGAAATTATAAGAGGTATGTTTGAAGAGAATGAACCAAGTTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAAAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCATGATTGCAAAACAATGATAGCCAGATTTGATCTCAATAATGATGGTAAAATTGATTTCCATGAATTTGTTAAGTTTATGGAAGTTGCTTTGTGTTAGAATATTTTGC

Coding sequence (CDS)

ATGTTCTTTAGATTAGAGTACCATTTAATTACAATGCAAACGCAGGTAACCAACGCTGTCAAGAGCGTGCAGCTCGACCCGTCACCGCTCGTTTTTGTCGATTGGATATCAAATCTACCAATTTTATTCAACTTTGCAGGCAGAATACATGAGCTGATTAGATCTCTACCTTATTGGTCAATTCTTCAATCACAGTACTCCAAAGAGAAAGCTTTATCACAAAGTAATTGCTCTTCACAATCTGATGATAAAATCATCATCACTACTCAACAAAATTGCTCAATGAGCAGAGAGGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGGAGGGAAGAAAATGATGATGATGATGATGATGAAGAAATTGGGGCAAGTGATGAAATTATAAGAGGTATGTTTGAAGAGAATGAACCAAGTTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAAAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCATGATTGCAAAACAATGATAGCCAGATTTGATCTCAATAATGATGGTAAAATTGATTTCCATGAATTTGTTAAGTTTATGGAAGTTGCTTTGTGTTAG

Protein sequence

MFFRLEYHLITMQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALC
Homology
BLAST of Pay0020929 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.1e-21
Identity = 68/179 (37.99%), Postives = 97/179 (54.19%), Query Frame = 0

Query: 32  FVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQ 91
           F  W+S+  I   F  R   L++        ++   +++ L++       DD        
Sbjct: 23  FCRWVSSTRI---FLSRFVPLLQHHQRVFDKKNNKDQQETLTKQEDDDDDDDDDDDDDDD 82

Query: 92  NCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTFNV 151
           +  +SREE   VM  L LF+    +DD   E+  A +  +  +FEE E SLEE+KQ F+V
Sbjct: 83  DIDISREEAEMVMRSLGLFY----NDDQLQEQYSAKE--VSSLFEEKEASLEEVKQAFDV 142

Query: 152 FDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVA 211
           FD NKDGFID  EL  VL++L   +G ++ +C  MI   D N DGKIDF+EFVKFME +
Sbjct: 143 FDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETS 192

BLAST of Pay0020929 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 6.5e-18
Identity = 69/215 (32.09%), Postives = 106/215 (49.30%), Query Frame = 0

Query: 7   YHLITMQTQVTNAVKSVQLDPSPLV---------FVDWISNLPILFNFAGRIHELIRSLP 66
           Y  ITM++ ++   +S  L   PL          F  W+S   + F+             
Sbjct: 7   YSFITMKSSLSKCKQSSSLS-FPLFGLINFFLIGFFRWVSFAQLFFS------------R 66

Query: 67  YWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDD 126
           +W ++Q Q    +  S+      S          +  + RE+V  VM+ L L      D 
Sbjct: 67  FWPLVQHQQCVSEKKSKDLEFQTSIKHEEYRDDDDDGLCREDVGMVMKSLGL----STDQ 126

Query: 127 DDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKG 186
           +++  +   S + +  +FEE EPSLEE+KQ F+VFD N+DGFID  +L  VL++L   +G
Sbjct: 127 ENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQG 186

Query: 187 IFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALC 213
             + +C+ MI  FD + DG+IDF+ FVKFME   C
Sbjct: 187 SNLENCRRMIRSFDGSKDGRIDFYGFVKFMENNFC 204

BLAST of Pay0020929 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.9e-10
Identity = 43/126 (34.13%), Postives = 74/126 (58.73%), Query Frame = 0

Query: 84  KIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLE 143
           K+++  + +   S EEV+ +++  E  +    ++ ++             + E+NE   +
Sbjct: 67  KVVVPNRADIKTSVEEVKAIIDDSEALYECLIEEGEE------------YLLEKNEMMGK 126

Query: 144 EL-KQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHE 203
           E+ K+ F +FD N+DGFIDE+EL  VLSLL  ++   + +C+ M+  +D N DGKIDF+E
Sbjct: 127 EIVKEAFRLFDENQDGFIDENELKHVLSLLGYDECTKM-ECRKMVKVYDENRDGKIDFYE 179

Query: 204 FVKFME 209
           FVK +E
Sbjct: 187 FVKLIE 179

BLAST of Pay0020929 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.8e-10
Identity = 39/111 (35.14%), Postives = 67/111 (60.36%), Query Frame = 0

Query: 98  EEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKD 157
           +E+  ++EK+++      D   D +E G   E+ + + +E +   E++K+ FNVFD+N D
Sbjct: 40  KELTQMIEKIDV----NGDGCVDIDEFG---ELYKTIMDEEDEEEEDMKEAFNVFDQNGD 99

Query: 158 GFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 209
           GFI   EL  VLS L   +G  + DCK MI + D++ DG++++ EF + M+
Sbjct: 100 GFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

BLAST of Pay0020929 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.4e-09
Identity = 39/106 (36.79%), Postives = 64/106 (60.38%), Query Frame = 0

Query: 103 VMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDE 162
           ++EK++L      D   D EE G    + + + EE +   E++++ FNVFD+N+DGFI  
Sbjct: 45  MIEKIDL----NGDGYVDIEEFGG---LYQTIMEERDEE-EDMREAFNVFDQNRDGFITV 104

Query: 163 HELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 209
            EL  VL+ L   +G  + DCK MI++ D++ DG ++F EF + M+
Sbjct: 105 EELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEFKQMMK 142

BLAST of Pay0020929 vs. ExPASy TrEMBL
Match: A0A1S3B8I1 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 PE=4 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 7.0e-108
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0

Query: 12  MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 71
           MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA
Sbjct: 1   MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 60

Query: 72  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEII 131
           LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEII
Sbjct: 61  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEII 120

Query: 132 RGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD 191
           RGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD
Sbjct: 121 RGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD 180

Query: 192 LNNDGKIDFHEFVKFMEVALC 213
           LNNDGKIDFHEFVKFMEVALC
Sbjct: 181 LNNDGKIDFHEFVKFMEVALC 201

BLAST of Pay0020929 vs. ExPASy TrEMBL
Match: A0A0A0LHQ5 (Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G825010 PE=4 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 6.1e-88
Identity = 179/212 (84.43%), Postives = 192/212 (90.57%), Query Frame = 0

Query: 1   MFFRLEYHLITMQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWS 60
           MFFRLEY+LITM+T++T+ +KSVQLDPSP VFVD IS LPILFNFA RI EL+RSLP W 
Sbjct: 1   MFFRLEYYLITMETRLTD-IKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLP-WP 60

Query: 61  ILQSQYSKEKALSQSNCSSQSDDKIIITTQQNC-SMSREEVRFVMEKLELFWREENDDDD 120
           ILQ  +SKEKALSQ NCSS+S D+IIITTQQNC SMSREEVRFVMEKLELFWREE D   
Sbjct: 61  ILQ-PHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGG 120

Query: 121 DDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIF 180
            D+ IG SDE+IRGMFEENEPSLEELKQTFNVFDRN+DGFIDEHELFIVLSLLESNKGIF
Sbjct: 121 GDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIF 180

Query: 181 IHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 212
           IHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL
Sbjct: 181 IHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 209

BLAST of Pay0020929 vs. ExPASy TrEMBL
Match: A0A6J1J5Q6 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111481990 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 5.6e-41
Identity = 113/185 (61.08%), Postives = 130/185 (70.27%), Query Frame = 0

Query: 27  PSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKII 86
           P PLVF+D  S       FA  IH+L+ SL + S  QS   KEKA  QS  +SQS+ K  
Sbjct: 21  PPPLVFIDRAS------IFADTIHDLV-SLAF-SFFQSP-PKEKAAPQSR-ASQSEGK-- 80

Query: 87  ITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELK 146
               ++  MSREEV+ VMEKLEL+  EE DD+ D+       E +RGMFEENEPSLEELK
Sbjct: 81  ----EDWLMSREEVKSVMEKLELYCNEEGDDEIDE---CFGREEVRGMFEENEPSLEELK 140

Query: 147 QTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKF 206
           QTFNVFDRN+DGFID HEL  VL LL SN  IFIHDCK+MIARFD NND KIDF+EFVKF
Sbjct: 141 QTFNVFDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNNDEKIDFNEFVKF 186

Query: 207 MEVAL 212
           MEV+L
Sbjct: 201 MEVSL 186

BLAST of Pay0020929 vs. ExPASy TrEMBL
Match: A0A6J1F854 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111441729 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 5.6e-41
Identity = 118/196 (60.20%), Postives = 136/196 (69.39%), Query Frame = 0

Query: 22  SVQLDPS------PLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQS 81
           ++QL PS      PLVF+D  S       FAG IH+L+ SL + S  QS   K+KA  QS
Sbjct: 9   NLQLIPSTTTTTPPLVFIDRAS------IFAGTIHDLV-SLAF-SFFQSP-PKDKAAPQS 68

Query: 82  NCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMF 141
             +SQS+ K      ++  MSREEV+ VMEKLEL+  EE DD+ D  E    DE +RGMF
Sbjct: 69  R-ASQSESK------EDWLMSREEVKSVMEKLELYCNEEEDDEID--ECFGRDE-VRGMF 128

Query: 142 EENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNND 201
           EENEPSLEELKQTFNVFDRN+DGFID  EL  VL LL SN  IFIHDCK+MIARFD NND
Sbjct: 129 EENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNND 185

Query: 202 GKIDFHEFVKFMEVAL 212
            KIDF+EFVKFMEVAL
Sbjct: 189 QKIDFNEFVKFMEVAL 185

BLAST of Pay0020929 vs. ExPASy TrEMBL
Match: A0A6J1EIK8 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111434428 PE=4 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 8.4e-37
Identity = 104/182 (57.14%), Postives = 124/182 (68.13%), Query Frame = 0

Query: 30  LVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKIIITT 89
           L+ VD IS   ILF+F  RIH+LI S P WS+  S   KEKA  Q N S +S+     T 
Sbjct: 16  LLLVDRISISSILFSFVDRIHDLI-SQP-WSLFHSP-PKEKASPQGN-SLESE----ATQ 75

Query: 90  QQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTF 149
            +N S+S+EEV+FVMEKLE F       D++ EE     E I  +F E+EPSLEELKQTF
Sbjct: 76  HKNSSISKEEVKFVMEKLEFFC-----SDEEIEESWLGGEEIGAIFYESEPSLEELKQTF 135

Query: 150 NVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEV 209
           NVFD+N+DG ID  EL  VL LL  N+G+FIHDC  MIARFD N DGKIDF+EFVKFME+
Sbjct: 136 NVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFMEI 184

Query: 210 AL 212
           AL
Sbjct: 196 AL 184

BLAST of Pay0020929 vs. NCBI nr
Match: XP_008443616.1 (PREDICTED: probable calcium-binding protein CML30 [Cucumis melo])

HSP 1 Score: 399.8 bits (1026), Expect = 1.4e-107
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0

Query: 12  MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 71
           MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA
Sbjct: 1   MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 60

Query: 72  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEII 131
           LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEII
Sbjct: 61  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEII 120

Query: 132 RGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD 191
           RGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD
Sbjct: 121 RGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD 180

Query: 192 LNNDGKIDFHEFVKFMEVALC 213
           LNNDGKIDFHEFVKFMEVALC
Sbjct: 181 LNNDGKIDFHEFVKFMEVALC 201

BLAST of Pay0020929 vs. NCBI nr
Match: KGN59556.1 (hypothetical protein Csa_001548 [Cucumis sativus])

HSP 1 Score: 333.6 bits (854), Expect = 1.3e-87
Identity = 179/212 (84.43%), Postives = 192/212 (90.57%), Query Frame = 0

Query: 1   MFFRLEYHLITMQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWS 60
           MFFRLEY+LITM+T++T+ +KSVQLDPSP VFVD IS LPILFNFA RI EL+RSLP W 
Sbjct: 1   MFFRLEYYLITMETRLTD-IKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLP-WP 60

Query: 61  ILQSQYSKEKALSQSNCSSQSDDKIIITTQQNC-SMSREEVRFVMEKLELFWREENDDDD 120
           ILQ  +SKEKALSQ NCSS+S D+IIITTQQNC SMSREEVRFVMEKLELFWREE D   
Sbjct: 61  ILQ-PHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGG 120

Query: 121 DDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIF 180
            D+ IG SDE+IRGMFEENEPSLEELKQTFNVFDRN+DGFIDEHELFIVLSLLESNKGIF
Sbjct: 121 GDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIF 180

Query: 181 IHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 212
           IHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL
Sbjct: 181 IHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 209

BLAST of Pay0020929 vs. NCBI nr
Match: XP_004147503.1 (probable calcium-binding protein CML45 [Cucumis sativus])

HSP 1 Score: 313.9 bits (803), Expect = 1.0e-81
Identity = 169/201 (84.08%), Postives = 181/201 (90.05%), Query Frame = 0

Query: 12  MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 71
           M+T++T+ +KSVQLDPSP VFVD IS LPILFNFA RI EL+RSLP W ILQ  +SKEKA
Sbjct: 1   METRLTD-IKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLP-WPILQ-PHSKEKA 60

Query: 72  LSQSNCSSQSDDKIIITTQQNC-SMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEI 131
           LSQ NCSS+S D+IIITTQQNC SMSREEVRFVMEKLELFWREE D    D+ IG SDE+
Sbjct: 61  LSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGESDEM 120

Query: 132 IRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF 191
           IRGMFEENEPSLEELKQTFNVFDRN+DGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF
Sbjct: 121 IRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF 180

Query: 192 DLNNDGKIDFHEFVKFMEVAL 212
           DLNNDGKIDFHEFVKFMEVAL
Sbjct: 181 DLNNDGKIDFHEFVKFMEVAL 198

BLAST of Pay0020929 vs. NCBI nr
Match: XP_038904514.1 (probable calcium-binding protein CML45 [Benincasa hispida])

HSP 1 Score: 234.2 bits (596), Expect = 1.0e-57
Identity = 138/192 (71.88%), Postives = 151/192 (78.65%), Query Frame = 0

Query: 22  SVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQS 81
           +VQL P+PLVFVD  S LPILF FA RIH+LI S+P  S  QS  SKEKA  QSNCSSQS
Sbjct: 9   NVQLIPTPLVFVDRTSILPILFTFADRIHDLI-SVPL-SFFQSP-SKEKASPQSNCSSQS 68

Query: 82  DDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGA--SDEIIRGMFEENE 141
           DD+ +IT Q+NC MSREEVRFVM+KLELF  +    +D DEEIG     E +  MFEENE
Sbjct: 69  DDQ-VITQQKNCLMSREEVRFVMDKLELFCSK----NDGDEEIGECFGGEELTAMFEENE 128

Query: 142 PSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKID 201
           PSLEELKQTFNVFDRN+DGFID  EL IVL LL+SN GIFIHDCK MIARFD NNDGKID
Sbjct: 129 PSLEELKQTFNVFDRNRDGFIDAEELHIVLGLLQSNNGIFIHDCKRMIARFDHNNDGKID 188

Query: 202 FHEFVKFMEVAL 212
           F EFVKFMEVAL
Sbjct: 189 FTEFVKFMEVAL 192

BLAST of Pay0020929 vs. NCBI nr
Match: XP_023528818.1 (probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 183.3 bits (464), Expect = 2.1e-42
Identity = 123/214 (57.48%), Postives = 140/214 (65.42%), Query Frame = 0

Query: 1   MFFRLEYHLITMQTQVT---NAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLP 60
           MFF+LEY L     Q T     + S      PLVF+D  S       FA  IH+LI  + 
Sbjct: 1   MFFKLEYLLTMHNPQPTINLQLIPSTTTTTPPLVFIDRAS------IFADTIHDLISLV- 60

Query: 61  YWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDD 120
            +S  QS   KEKA  QS  +SQS+ K      ++  MSREEV+ VMEKLEL+  EE DD
Sbjct: 61  -FSFFQSP-PKEKAAPQSR-ASQSEGK------EDWLMSREEVKSVMEKLELYCNEEEDD 120

Query: 121 DDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKG 180
           + D  E    DE +RGMFEENEPSLEELKQTFNVFDRN+DGFID  EL  VL LL SN  
Sbjct: 121 EID--ECFGRDE-VRGMFEENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNT 180

Query: 181 IFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 212
           IFIHDCK+MIARFD NND KIDF+EFVKFMEVAL
Sbjct: 181 IFIHDCKSMIARFDHNNDQKIDFNEFVKFMEVAL 195

BLAST of Pay0020929 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.5e-22
Identity = 68/179 (37.99%), Postives = 97/179 (54.19%), Query Frame = 0

Query: 32  FVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQ 91
           F  W+S+  I   F  R   L++        ++   +++ L++       DD        
Sbjct: 23  FCRWVSSTRI---FLSRFVPLLQHHQRVFDKKNNKDQQETLTKQEDDDDDDDDDDDDDDD 82

Query: 92  NCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTFNV 151
           +  +SREE   VM  L LF+    +DD   E+  A +  +  +FEE E SLEE+KQ F+V
Sbjct: 83  DIDISREEAEMVMRSLGLFY----NDDQLQEQYSAKE--VSSLFEEKEASLEEVKQAFDV 142

Query: 152 FDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVA 211
           FD NKDGFID  EL  VL++L   +G ++ +C  MI   D N DGKIDF+EFVKFME +
Sbjct: 143 FDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETS 192

BLAST of Pay0020929 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 92.4 bits (228), Expect = 4.6e-19
Identity = 69/215 (32.09%), Postives = 106/215 (49.30%), Query Frame = 0

Query: 7   YHLITMQTQVTNAVKSVQLDPSPLV---------FVDWISNLPILFNFAGRIHELIRSLP 66
           Y  ITM++ ++   +S  L   PL          F  W+S   + F+             
Sbjct: 7   YSFITMKSSLSKCKQSSSLS-FPLFGLINFFLIGFFRWVSFAQLFFS------------R 66

Query: 67  YWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDD 126
           +W ++Q Q    +  S+      S          +  + RE+V  VM+ L L      D 
Sbjct: 67  FWPLVQHQQCVSEKKSKDLEFQTSIKHEEYRDDDDDGLCREDVGMVMKSLGL----STDQ 126

Query: 127 DDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKG 186
           +++  +   S + +  +FEE EPSLEE+KQ F+VFD N+DGFID  +L  VL++L   +G
Sbjct: 127 ENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQG 186

Query: 187 IFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALC 213
             + +C+ MI  FD + DG+IDF+ FVKFME   C
Sbjct: 187 SNLENCRRMIRSFDGSKDGRIDFYGFVKFMENNFC 204

BLAST of Pay0020929 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.1e-11
Identity = 43/126 (34.13%), Postives = 74/126 (58.73%), Query Frame = 0

Query: 84  KIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLE 143
           K+++  + +   S EEV+ +++  E  +    ++ ++             + E+NE   +
Sbjct: 67  KVVVPNRADIKTSVEEVKAIIDDSEALYECLIEEGEE------------YLLEKNEMMGK 126

Query: 144 EL-KQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHE 203
           E+ K+ F +FD N+DGFIDE+EL  VLSLL  ++   + +C+ M+  +D N DGKIDF+E
Sbjct: 127 EIVKEAFRLFDENQDGFIDENELKHVLSLLGYDECTKM-ECRKMVKVYDENRDGKIDFYE 179

Query: 204 FVKFME 209
           FVK +E
Sbjct: 187 FVKLIE 179

BLAST of Pay0020929 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 66.6 bits (161), Expect = 2.7e-11
Identity = 39/111 (35.14%), Postives = 67/111 (60.36%), Query Frame = 0

Query: 98  EEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKD 157
           +E+  ++EK+++      D   D +E G   E+ + + +E +   E++K+ FNVFD+N D
Sbjct: 40  KELTQMIEKIDV----NGDGCVDIDEFG---ELYKTIMDEEDEEEEDMKEAFNVFDQNGD 99

Query: 158 GFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 209
           GFI   EL  VLS L   +G  + DCK MI + D++ DG++++ EF + M+
Sbjct: 100 GFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

BLAST of Pay0020929 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 64.7 bits (156), Expect = 1.0e-10
Identity = 39/106 (36.79%), Postives = 64/106 (60.38%), Query Frame = 0

Query: 103 VMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDE 162
           ++EK++L      D   D EE G    + + + EE +   E++++ FNVFD+N+DGFI  
Sbjct: 45  MIEKIDL----NGDGYVDIEEFGG---LYQTIMEERDEE-EDMREAFNVFDQNRDGFITV 104

Query: 163 HELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 209
            EL  VL+ L   +G  + DCK MI++ D++ DG ++F EF + M+
Sbjct: 105 EELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEFKQMMK 142

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9MBG52.1e-2137.99Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ... [more]
Q93Z276.5e-1832.09Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ... [more]
Q9SN892.9e-1034.13Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ... [more]
Q9LNE73.8e-1035.14Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1[more]
Q9SRR71.4e-0936.79Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3B8I17.0e-108100.00probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 P... [more]
A0A0A0LHQ56.1e-8884.43Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G82501... [more]
A0A6J1J5Q65.6e-4161.08probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114819... [more]
A0A6J1F8545.6e-4160.20probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1EIK88.4e-3757.14probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
Match NameE-valueIdentityDescription
XP_008443616.11.4e-107100.00PREDICTED: probable calcium-binding protein CML30 [Cucumis melo][more]
KGN59556.11.3e-8784.43hypothetical protein Csa_001548 [Cucumis sativus][more]
XP_004147503.11.0e-8184.08probable calcium-binding protein CML45 [Cucumis sativus][more]
XP_038904514.11.0e-5771.88probable calcium-binding protein CML45 [Benincasa hispida][more]
XP_023528818.12.1e-4257.48probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G29000.11.5e-2237.99Calcium-binding EF-hand family protein [more]
AT5G39670.14.6e-1932.09Calcium-binding EF-hand family protein [more]
AT3G47480.12.1e-1134.13Calcium-binding EF-hand family protein [more]
AT1G05990.12.7e-1135.14EF hand calcium-binding protein family [more]
AT3G07490.11.0e-1036.79ARF-GAP domain 11 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 182..210
e-value: 8.7E-4
score: 28.6
coord: 144..172
e-value: 2.2E-5
score: 33.9
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 143..208
e-value: 1.6E-9
score: 38.1
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 178..212
score: 11.054376
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 140..175
score: 13.760229
IPR002048EF-hand domainCDDcd00051EFhcoord: 144..207
e-value: 2.26279E-15
score: 66.0321
NoneNo IPR availableGENE3D1.10.238.10coord: 69..210
e-value: 7.0E-19
score: 70.4
NoneNo IPR availablePANTHERPTHR10891:SF745CALCIUM-BINDING PROTEIN CML46-RELATEDcoord: 16..208
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 16..208
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 191..203
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 153..165
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 136..207

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0020929.1Pay0020929.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding