Pay0020002 (gene) Melon (Payzawat) v1

Overview
NamePay0020002
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description4-coumarate CoA ligase
Locationchr11: 7293149 .. 7298504 (+)
RNA-Seq ExpressionPay0020002
SyntenyPay0020002
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTAAGATTGATAGTTGGATTTGCCTCCACCGTTTCTTCTACCTACCCTTTTCCTTTTTTCTTTGGAACCGTTCTTTTCCTCTTATTTATCAATCACTCCCTTCCTTTTTTTCTCTATGCAAAACCAAACTCCCATTGAACCCGATTTAACGTTTTCATTGCCACTCGTCCTCCCCCCTTTTACCAGCCATGATTTCCCTAGCCCCAGTATGCGACCCCCAACCACCCAATAATGTCTCCTCCGATTTCTCATCCTCTCCCCCGCCCTCCACGCCGGCCACCCATGTTTTCCGATCCAAATTACCCGACATTCCTATCCCCGACCATCTCCCTCTTCACAGTTACTGCTTCCAGAAACTCTCCCAAGTTTCCGACCGCCCATGTTTGATCGTCGGCTCCACCGGAAAATCTTATTCCTACTCCGAAACCCACCTCTTTTCCCGCAAAGCCGCCGCCACTTTCTCTAAACTTGGTGTTCAGAGAGGGGATGTTATTATGATTCTCCTCCATAATTCTCCTGAATTTATTTTCTCTTTTATGGGTTCCTCCATGCTCGGCGCCATCGCTACCACCGCCAATCCTTACTACACGGGGGCCGAGATTTCTAAGCAGTTGAAGGCCTCCGGTGCTAAATTCGTTGTTACTTACTCCCGATGCGTTGATAAGTTACGAGAATGTGGTGAAGTTCTCACTATTGTCACCGTGGATGACCCGCCGGAGAATTGTCTGAGCTTCTCGATGGTTTACGACGCCAATGAGAACGATGTCCCGTTTGTGGAGATTGATACGAACGATGCCGTTTCCCTACCGTTCTCCTCCGGCACGACCGGGCTCCCCAAGGGTGTGATTTTGACTCATAAGAACATGGTGTCGAGTGTGGCTCAACAGGTTCTTATTATAAACTTTGTATATAATATTTTGGTTAAAATGTCCATATTTAATTTAACATTTAATCACATTTTTTATACTAATTGATTAAGAATATTTCATAATAATTTAAATAATTTTAAATTCACTATGTATCATCCGTTGTGACAAACAGTATGATAGAATGAAAAGAAAATGGGTAATTCTAGTATATTTGAAAAATATTTAATCATTTATTCATATTGTAAATCTGCAGGTGGATGGAGAAAACCCGAACCTATATTTAAAAAGAAACGACATAGTTTTATGCGTGTTACCAATGTTTCATATATTCTCATTGAGTAGCATAGTTTTGATATCAATCAGATCCGGGGCAGCGTTGCTTTTGATGGAGAAATTTGAGATCGAGTCATTGCTACGACTAGTAGAGAGACACAAGGTGACGGTGGCAACGGTGGTGCCGCCGCTCGTGGTGTCGCTAGTGAAGAACCCGAAGGTTGCAGATTTCAACTTGAGCTCCATTCGATTAGTGCTTTCCGGAGCAGCACCGTTGAGAAAGGAGCTGGAGGAGGCTCTCATGGAAAGGCTTCCTCAAGCCATTTTTGGTCAGGTATGTGGTAATGAAATGACTATAAAGTATGGGATAACTAAAGGCTATTAAGTTTTATAGATGTAGCATGTGAAAGTAATTAAATAAAAAGGAAAAAAGACTTTTATTTTGCAATCTCTTGCGATAAAAGAAAAACGGTAGAATTATGTACCATGGAATCTAATTTTATATCAATGCTATATCACGTTACATACTTTCTCTTATAGAAATGTCTAGTGATATTCACTAAACCAATCAAATTTCGCATATATAAATATAAATGTTATTAGATAGTCAGCAACAAAATAATTGTAAGAAAACATCCTCCGCTTACTTAATTCTATATTCATTTATTTTTAAATAGTATCTTGTTCCAACAATAACTATCTTCTATATAATATGATCAATGAATAATCATTTTAATTAAAGTCAATTTTTAAGTCGTCCGAAAGTTCGGTGATCTTGAATCTATTAAAAAAGAACCATAGTTTTGGTAAAAGGACAATATAAATAGACGTTGAAAACTCGAACCTTTTGAATGAAAATGACATGTTTAATTAACATTAATTTAAAGTTCTTTACTAACTAATTGATATTTGGACATTAATATGTGGAACATGACCTAATAAATAGGGTTATGGTATGACGGAAGCGGGTCCCGTGCTGAGCATGTGCTCGGCTTTTGCAAAGGAGCCACCAATGCCAACAAAATCTGGCTCCTGCGGCCGAGTTGTTAGAAACTCGGAGCTTAAAGTCGTTGACCCACTAACCGGTGCCTCACTCACTTATAATCAACCCGGAGAAATTTGTGTTCGAGGCCCCCAAGTTATGAAAGGTTTTTTTTCTTTTTTCTTTTCTTCTCACTTAATTAATTATTCTATTCGGTGAAATTCTGACTAATTTTTCTATCCATTTTATGAAAATAACTTGAAATTCTTTAAAAGCTATTTTCTTGGGTTGAATAATATAATTAAACTTACTTGTTGAGTCTCTTTAGAAATAATTTTTAGATGGACAATTCACATTATCCAATAAGGTATAATTAGAAGAATAAGAAATGGATGATGAATATAATTAATGTTAAATTGAATGAAGTAGAAGTAATTAGGGAATAAAAGTAGGAGTACACACTGGTGTAGAACGAGTTTGTGTGTTTACCATTAAAATTGTGAAAATGGAACAAAGTACTTAATAATTTTGGTGTTAAATTGAGATGGGTGTGATGATATTGTAGGATATTTGAATGACCCGGTGTCCACGTCATTGACTGTCGATGTGGAGGGTTGGCTTCATACGGGTGACATTGGTTACGTTGACGATGAAGAAGAAATTTTTATTGTTGATAGAGTTAAAGAGATTATTAAATTTAAAGGTTTTCAGGTATAAACTATTCCCTAAAATTCTAATATTTATTTTCTGTTTAATCCTTTTTAACTCTTATTTTTTTGTTGTTGTTATTTACTGAATTTAATTAAATGTTATATTTTTAACTTCTTAGAAAATAATAATAATAATAATTGAAAATTTTTATTTGGTTTCTAAATTTTAAAATGTTATAATAGATTACGGTTATATTAGTTTGAATATTTTGAGATTTTTAGTCGATAATGTGTATTTGTTGTGCTTCTAATTAAGTAATTAAAATAATTGATGAGTTAAATTTTCATTTTAATTTTAATAGTGAGAAAAAATTAGTGAACATTAATCATTTAAATTATTTGATTACTTGAATGCTAACAGACATTACTATTAAAGGATACGAGGCTATAGTTAGTATAGCACAAAGTATTTTAAAAATTGATACTACTTTCAACCTTGTACAATTTAAAATGATCATATGTTTAAAATTATTGGTAATGGTTTATACTCGGTAAAATTATTAAAACTATTTTCGAAATAAAGTATTGTATGATTGTCTATTAGGGTTTGTATTTCTTTCGCGAGAGACAATCATATTTTATTAAATTTCTCAATAAAAAGTTAGTTCAATTAATATATGTATGTACCGGAATCATCAAGGGGTCTATGGTCTAAATCCGCATTCTCCTTTAAATAAAAATAAAATCTCATTTTCTTGGTTCCAATTAAAGAATAGATTTTATTTCTAAACAAATTAAGAAGCAAGACTTCCTAACAAGAGTAGTTTTGGAGTGTAGAATTGGTTCAATTTTTGTGTAATTAATTGACTGACAAGTTGGTTGAGTTATTTATTTTTACCAAATTAATTTTCTATTAATGCAATTATCTTCATATATAAAATCTCTCATGGTATCTGAGGTTCAATCCCCCCTTTGTTGTTGTTAAAAAAACTCTATACATGAAATTTATAAAATTATGATATCTTTTATAGGTGGCACCGGCAGAGTTAGAGGCTCTTCTTGTAACCCATACATCTATCGTTGATGCTGCTGTTGTCCCGTATGTAAACAATTTAAATTTTTCAATTTTCTTTTTTGGGTAATTGCTTTAAAGGATAGAATTGTTAAAAATATTTTCAATTACAGGCAAAATGATGATGTTGCTGGTGAAGTTCCAGTAGCTTTCGTAGTTCCATCAACTCAGAATGGACTTACAGAGGAATCAGTAAAAGAATTCATAGCAAAGCAGGTTTGTGTGCTATCAATTTAATTTCCCTTATAATCAAATTATAATTATAGTTCACGTTTAAATTTCAAAGATAATATGTTTAAAAAAATTTGAGAAAAAAATAAAATGTTAAAACTATTTATTATTTTTCTAATGTATTATTCAATAAAAGTTTATATTGCAAATATTCTTTTTTTTTTTTTAACATTATTTTCACCCTCAACTTTTGTTTTCGATTGAAGTTAAGCTTATTTGTTTAAAACTATAGAAACTACTTTTTAGTTTGACAATTACTTTTGTTTTTAGTATCAATTTTGTTTTAAGTTTTTTTTTTAATTTACTTTTCTTTGGTCTCGAATTTTATATTATTAATTCTAAAATTTCTTGAAAAACGTTGAATTGTTAGTTAAATTTTAAAAATAAAAACAAACTTTTTTTTTAATAGGATATCGTTTCATAAGTATGACATTTTTTATTTTTTTATTTTTCAACACTAAACTTATTTTTTCTCATTTTCTTATAATAATTTGTAATTTTCTTTTTCCGATAATTGAATTCTTAGTTAAATATTCTACAGATGAAAAAGTAATTTTTTAAATTTCAAATTGACGTTGTTTTTCAAATATTGGTAGAAAAAGATAATGCAAAAATAAATATAGAGGTGGGGGATATAGTTACATAATTCTATAAAACTAGAAATAAAAAATCAAATCTATGGCTATATCATAATCAAATAGACCTAGAGCTTTTTGATTTTTCTTTAATTTTGGAGTCATATTGAAAAGACATGGCATATATAATTATTAACTTTTCTTAGAGTAATAATAAAGAAAATTAGTTGTGAAATCAAGTGTTGTTAAATCACAATAAAAGTTCAAACAGTAGTTTGTACAAAGGGAATTGAAGCATTGAATTGATATCTATCTCACGTTGGTTATTTACTTTTTAAATGCAAAACCAACAGGTTGTGTTCTACAAGAGATTGCACAAAGTATATTTTGTGAAGACCATTCCAAAATCACCTTCTGGAAAGACCTTGAGAAAGGAACTCAAAGCCAAACTCTCATAAGTCTTTTTAAAGCCACAGGAATAGAGACATGAAGAGGTAGAGTTATTATTAATTGTCATTAATGCTTGTCTATGTCTATCTCTTCAACCCTTGATTGTATACCAACTTTTGTTATGTGCAATAATGTGAATGTCTTCAATTTATTATTATTAATTTTGTGGTCTTTAGCCATCTTTCTTTATGAGTTTTGCACATAAAAGACGATGTGGTGTGGCCTTCTATATTAATTTTATGGTTCCATTAG

mRNA sequence

CTTTAAGATTGATAGTTGGATTTGCCTCCACCGTTTCTTCTACCTACCCTTTTCCTTTTTTCTTTGGAACCGTTCTTTTCCTCTTATTTATCAATCACTCCCTTCCTTTTTTTCTCTATGCAAAACCAAACTCCCATTGAACCCGATTTAACGTTTTCATTGCCACTCGTCCTCCCCCCTTTTACCAGCCATGATTTCCCTAGCCCCAGTATGCGACCCCCAACCACCCAATAATGTCTCCTCCGATTTCTCATCCTCTCCCCCGCCCTCCACGCCGGCCACCCATGTTTTCCGATCCAAATTACCCGACATTCCTATCCCCGACCATCTCCCTCTTCACAGTTACTGCTTCCAGAAACTCTCCCAAGTTTCCGACCGCCCATGTTTGATCGTCGGCTCCACCGGAAAATCTTATTCCTACTCCGAAACCCACCTCTTTTCCCGCAAAGCCGCCGCCACTTTCTCTAAACTTGGTGTTCAGAGAGGGGATGTTATTATGATTCTCCTCCATAATTCTCCTGAATTTATTTTCTCTTTTATGGGTTCCTCCATGCTCGGCGCCATCGCTACCACCGCCAATCCTTACTACACGGGGGCCGAGATTTCTAAGCAGTTGAAGGCCTCCGGTGCTAAATTCGTTGTTACTTACTCCCGATGCGTTGATAAGTTACGAGAATGTGGTGAAGTTCTCACTATTGTCACCGTGGATGACCCGCCGGAGAATTGTCTGAGCTTCTCGATGGTTTACGACGCCAATGAGAACGATGTCCCGTTTGTGGAGATTGATACGAACGATGCCGTTTCCCTACCGTTCTCCTCCGGCACGACCGGGCTCCCCAAGGGTGTGATTTTGACTCATAAGAACATGGTGTCGAGTGTGGCTCAACAGGTGGATGGAGAAAACCCGAACCTATATTTAAAAAGAAACGACATAGTTTTATGCGTGTTACCAATGTTTCATATATTCTCATTGAGTAGCATAGTTTTGATATCAATCAGATCCGGGGCAGCGTTGCTTTTGATGGAGAAATTTGAGATCGAGTCATTGCTACGACTAGTAGAGAGACACAAGGTGACGGTGGCAACGGTGGTGCCGCCGCTCGTGGTGTCGCTAGTGAAGAACCCGAAGGTTGCAGATTTCAACTTGAGCTCCATTCGATTAGTGCTTTCCGGAGCAGCACCGTTGAGAAAGGAGCTGGAGGAGGCTCTCATGGAAAGGCTTCCTCAAGCCATTTTTGGTCAGGGTTATGGTATGACGGAAGCGGGTCCCGTGCTGAGCATGTGCTCGGCTTTTGCAAAGGAGCCACCAATGCCAACAAAATCTGGCTCCTGCGGCCGAGTTGTTAGAAACTCGGAGCTTAAAGTCGTTGACCCACTAACCGGTGCCTCACTCACTTATAATCAACCCGGAGAAATTTGTGTTCGAGGCCCCCAAGTTATGAAAGGATATTTGAATGACCCGGTGTCCACGTCATTGACTGTCGATGTGGAGGGTTGGCTTCATACGGGTGACATTGGTTACGTTGACGATGAAGAAGAAATTTTTATTGTTGATAGAGTTAAAGAGATTATTAAATTTAAAGGTTTTCAGGTGGCACCGGCAGAGTTAGAGGCTCTTCTTGTAACCCATACATCTATCGTTGATGCTGCTGTTGTCCCGCAAAATGATGATGTTGCTGGTGAAGTTCCAGTAGCTTTCGTAGTTCCATCAACTCAGAATGGACTTACAGAGGAATCAGTAAAAGAATTCATAGCAAAGCAGGTTGTGTTCTACAAGAGATTGCACAAAGTATATTTTGTGAAGACCATTCCAAAATCACCTTCTGGAAAGACCTTGAGAAAGGAACTCAAAGCCAAACTCTCATAAGTCTTTTTAAAGCCACAGGAATAGAGACATGAAGAGGTAGAGTTATTATTAATTGTCATTAATGCTTGTCTATGTCTATCTCTTCAACCCTTGATTGTATACCAACTTTTGTTATGTGCAATAATGTGAATGTCTTCAATTTATTATTATTAATTTTGTGGTCTTTAGCCATCTTTCTTTATGAGTTTTGCACATAAAAGACGATGTGGTGTGGCCTTCTATATTAATTTTATGGTTCCATTAG

Coding sequence (CDS)

ATGATTTCCCTAGCCCCAGTATGCGACCCCCAACCACCCAATAATGTCTCCTCCGATTTCTCATCCTCTCCCCCGCCCTCCACGCCGGCCACCCATGTTTTCCGATCCAAATTACCCGACATTCCTATCCCCGACCATCTCCCTCTTCACAGTTACTGCTTCCAGAAACTCTCCCAAGTTTCCGACCGCCCATGTTTGATCGTCGGCTCCACCGGAAAATCTTATTCCTACTCCGAAACCCACCTCTTTTCCCGCAAAGCCGCCGCCACTTTCTCTAAACTTGGTGTTCAGAGAGGGGATGTTATTATGATTCTCCTCCATAATTCTCCTGAATTTATTTTCTCTTTTATGGGTTCCTCCATGCTCGGCGCCATCGCTACCACCGCCAATCCTTACTACACGGGGGCCGAGATTTCTAAGCAGTTGAAGGCCTCCGGTGCTAAATTCGTTGTTACTTACTCCCGATGCGTTGATAAGTTACGAGAATGTGGTGAAGTTCTCACTATTGTCACCGTGGATGACCCGCCGGAGAATTGTCTGAGCTTCTCGATGGTTTACGACGCCAATGAGAACGATGTCCCGTTTGTGGAGATTGATACGAACGATGCCGTTTCCCTACCGTTCTCCTCCGGCACGACCGGGCTCCCCAAGGGTGTGATTTTGACTCATAAGAACATGGTGTCGAGTGTGGCTCAACAGGTGGATGGAGAAAACCCGAACCTATATTTAAAAAGAAACGACATAGTTTTATGCGTGTTACCAATGTTTCATATATTCTCATTGAGTAGCATAGTTTTGATATCAATCAGATCCGGGGCAGCGTTGCTTTTGATGGAGAAATTTGAGATCGAGTCATTGCTACGACTAGTAGAGAGACACAAGGTGACGGTGGCAACGGTGGTGCCGCCGCTCGTGGTGTCGCTAGTGAAGAACCCGAAGGTTGCAGATTTCAACTTGAGCTCCATTCGATTAGTGCTTTCCGGAGCAGCACCGTTGAGAAAGGAGCTGGAGGAGGCTCTCATGGAAAGGCTTCCTCAAGCCATTTTTGGTCAGGGTTATGGTATGACGGAAGCGGGTCCCGTGCTGAGCATGTGCTCGGCTTTTGCAAAGGAGCCACCAATGCCAACAAAATCTGGCTCCTGCGGCCGAGTTGTTAGAAACTCGGAGCTTAAAGTCGTTGACCCACTAACCGGTGCCTCACTCACTTATAATCAACCCGGAGAAATTTGTGTTCGAGGCCCCCAAGTTATGAAAGGATATTTGAATGACCCGGTGTCCACGTCATTGACTGTCGATGTGGAGGGTTGGCTTCATACGGGTGACATTGGTTACGTTGACGATGAAGAAGAAATTTTTATTGTTGATAGAGTTAAAGAGATTATTAAATTTAAAGGTTTTCAGGTGGCACCGGCAGAGTTAGAGGCTCTTCTTGTAACCCATACATCTATCGTTGATGCTGCTGTTGTCCCGCAAAATGATGATGTTGCTGGTGAAGTTCCAGTAGCTTTCGTAGTTCCATCAACTCAGAATGGACTTACAGAGGAATCAGTAAAAGAATTCATAGCAAAGCAGGTTGTGTTCTACAAGAGATTGCACAAAGTATATTTTGTGAAGACCATTCCAAAATCACCTTCTGGAAAGACCTTGAGAAAGGAACTCAAAGCCAAACTCTCATAA

Protein sequence

MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQVSDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSSMLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCLSFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATVVPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTIPKSPSGKTLRKELKAKLS
Homology
BLAST of Pay0020002 vs. ExPASy Swiss-Prot
Match: P31687 (4-coumarate--CoA ligase 2 OS=Glycine max OX=3847 PE=2 SV=2)

HSP 1 Score: 751.5 bits (1939), Expect = 6.7e-216
Identity = 387/563 (68.74%), Postives = 460/563 (81.71%), Query Frame = 0

Query: 1   MISLAPVCD-PQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQ 60
           MI+LAP  D P+   N  SD      P T  +HVF+SKLPDIPI +HLPLHSYCFQ LSQ
Sbjct: 1   MITLAPSLDTPKTDQNQVSD------PQT--SHVFKSKLPDIPISNHLPLHSYCFQNLSQ 60

Query: 61  VSDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGS 120
            + RPCLIVG   K+++Y++THL S K AA  S LG+ +GDV+MILL NS +F+FSF+  
Sbjct: 61  FAHRPCLIVGPASKTFTYADTHLISSKIAAGLSNLGILKGDVVMILLQNSADFVFSFLAI 120

Query: 121 SMLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLR-----ECGEVLTIVTVDD 180
           SM+GA+ATTANP+YT  EI KQ   S AK ++T +  VDKLR     + GE   +VTVDD
Sbjct: 121 SMIGAVATTANPFYTAPEIFKQFTVSKAKLIITQAMYVDKLRNHDGAKLGEDFKVVTVDD 180

Query: 181 PPENCLSFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQV 240
           PPENCL FS++ +ANE+DVP VEI  +DAV++PFSSGTTGLPKGVILTHK++ +SVAQQV
Sbjct: 181 PPENCLHFSVLSEANESDVPEVEIHPDDAVAMPFSSGTTGLPKGVILTHKSLTTSVAQQV 240

Query: 241 DGENPNLYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHK 300
           DGENPNLYL   D++LCVLP+FHIFSL+S++L ++R+G+A+LLM+KFEI +LL L++RH+
Sbjct: 241 DGENPNLYLTTEDVLLCVLPLFHIFSLNSVLLCALRAGSAVLLMQKFEIGTLLELIQRHR 300

Query: 301 VTVATVVPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYG 360
           V+VA VVPPLV++L KNP VADF+LSSIRLVLSGAAPL KELEEAL  R+PQA+ GQGYG
Sbjct: 301 VSVAMVVPPLVLALAKNPMVADFDLSSIRLVLSGAAPLGKELEEALRNRMPQAVLGQGYG 360

Query: 361 MTEAGPVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGP 420
           MTEAGPVLSMC  FAK+ P  TKSGSCG VVRN+ELKVVDP TG SL YNQPGEIC+RG 
Sbjct: 361 MTEAGPVLSMCLGFAKQ-PFQTKSGSCGTVVRNAELKVVDPETGRSLGYNQPGEICIRGQ 420

Query: 421 QVMKGYLNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELE 480
           Q+MKGYLND  +T+ T+D EGWLHTGD+GYVDD++EIFIVDRVKE+IK+KGFQV PAELE
Sbjct: 421 QIMKGYLNDEAATASTIDSEGWLHTGDVGYVDDDDEIFIVDRVKELIKYKGFQVPPAELE 480

Query: 481 ALLVTHTSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKV 540
            LLV+H SI DAAVVPQ D  AGEVPVAFVV S    LTEE+VKEFIAKQVVFYKRLHKV
Sbjct: 481 GLLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGFDLTEEAVKEFIAKQVVFYKRLHKV 540

Query: 541 YFVKTIPKSPSGKTLRKELKAKL 558
           YFV  IPKSPSGK LRK+L+AKL
Sbjct: 541 YFVHAIPKSPSGKILRKDLRAKL 554

BLAST of Pay0020002 vs. ExPASy Swiss-Prot
Match: Q9S777 (4-coumarate--CoA ligase 3 OS=Arabidopsis thaliana OX=3702 GN=4CL3 PE=1 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 8.2e-214
Identity = 378/559 (67.62%), Postives = 463/559 (82.83%), Query Frame = 0

Query: 5   APVCDPQ--PPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQVSD 64
           A + +PQ   P + S       P S P   +FRSKLPDI IP+HLPLH+YCF+KLS VSD
Sbjct: 4   AALHEPQIHKPTDTSVVSDDVLPHSPPTPRIFRSKLPDIDIPNHLPLHTYCFEKLSSVSD 63

Query: 65  RPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSSML 124
           +PCLIVGSTGKSY+Y ETHL  R+ A+   KLG+++GDVIMILL NS EF+FSFMG+SM+
Sbjct: 64  KPCLIVGSTGKSYTYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMI 123

Query: 125 GAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDP-PENCLS 184
           GA++TTANP+YT  E+ KQLK+SGAK ++T+S+ VDKL+  GE LT++T D+P PENCL 
Sbjct: 124 GAVSTTANPFYTSQELYKQLKSSGAKLIITHSQYVDKLKNLGENLTLITTDEPTPENCLP 183

Query: 185 FSMVYDANENDVPF---VEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGEN 244
           FS +   +E + PF   V+I  +DA +LPFSSGTTGLPKGV+LTHK++++SVAQQVDG+N
Sbjct: 184 FSTLITDDETN-PFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDN 243

Query: 245 PNLYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVA 304
           PNLYLK ND++LCVLP+FHI+SL+S++L S+RSGA +LLM KFEI +LL L++RH+VT+A
Sbjct: 244 PNLYLKSNDVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIA 303

Query: 305 TVVPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEA 364
            +VPPLV++L KNP V  ++LSS+R VLSGAAPL KEL+++L  RLPQAI GQGYGMTEA
Sbjct: 304 ALVPPLVIALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEA 363

Query: 365 GPVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMK 424
           GPVLSM   FAKE P+PTKSGSCG VVRN+ELKVV   T  SL YNQPGEIC+RG Q+MK
Sbjct: 364 GPVLSMSLGFAKE-PIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMK 423

Query: 425 GYLNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLV 484
            YLNDP +TS T+D EGWLHTGDIGYVD+++EIFIVDR+KE+IKFKGFQV PAELE+LL+
Sbjct: 424 EYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLI 483

Query: 485 THTSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVK 544
            H SI DAAVVPQND+VAGEVPVAFVV S  N +TEE VKE++AKQVVFYKRLHKV+FV 
Sbjct: 484 NHHSIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVA 543

Query: 545 TIPKSPSGKTLRKELKAKL 558
           +IPKSPSGK LRK+LKAKL
Sbjct: 544 SIPKSPSGKILRKDLKAKL 560

BLAST of Pay0020002 vs. ExPASy Swiss-Prot
Match: Q42982 (4-coumarate--CoA ligase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CL2 PE=1 SV=2)

HSP 1 Score: 686.8 bits (1771), Expect = 2.0e-196
Identity = 356/568 (62.68%), Postives = 438/568 (77.11%), Query Frame = 0

Query: 7   VCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQVSDRPCL 66
           V  P+    V++    +PP    A  VFRSKLPDI IP HLPLH YCF + +++ D PCL
Sbjct: 4   VAAPEAQPQVAAAVDEAPP---EAVTVFRSKLPDIDIPSHLPLHEYCFARAAELPDAPCL 63

Query: 67  IVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSSMLGAIA 126
           I  +TG++Y+++ET L  R+AAA   +LGV  GD +M+LL N  EF  +F  +S LGA+ 
Sbjct: 64  IAAATGRTYTFAETRLLCRRAAAALHRLGVGHGDRVMVLLQNCVEFAVAFFAASFLGAVT 123

Query: 127 TTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRE---------C---GEVLTIVTVDD 186
           T ANP+ T  EI KQ KASG K ++T S  VDKLR+         C    + LT++T+DD
Sbjct: 124 TAANPFCTPQEIHKQFKASGVKLILTQSVYVDKLRQHEAFPRIDACTVGDDTLTVITIDD 183

Query: 187 ---PPENCLSF-SMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSV 246
               PE CL F  ++ DA+E  VP V I  +D V+LPFSSGTTGLPKGV+LTH+++VS V
Sbjct: 184 DEATPEGCLPFWDLIADADEGSVPEVAISPDDPVALPFSSGTTGLPKGVVLTHRSVVSGV 243

Query: 247 AQQVDGENPNLYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLV 306
           AQQVDGENPNL++   D+ LCVLP+FHIFSL+S++L ++R+GAA+ LM +FE+ ++L  +
Sbjct: 244 AQQVDGENPNLHMGAGDVALCVLPLFHIFSLNSVLLCAVRAGAAVALMPRFEMGAMLGAI 303

Query: 307 ERHKVTVATVVPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFG 366
           ER +VTVA VVPPLV++L KNP V   +LSSIR+VLSGAAPL KELE+AL  RLPQAIFG
Sbjct: 304 ERWRVTVAAVVPPLVLALAKNPFVERHDLSSIRIVLSGAAPLGKELEDALRARLPQAIFG 363

Query: 367 QGYGMTEAGPVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEIC 426
           QGYGMTEAGPVLSMC AFAKE P P KSGSCG VVRN+ELKVVDP TG SL  N PGEIC
Sbjct: 364 QGYGMTEAGPVLSMCPAFAKE-PTPAKSGSCGTVVRNAELKVVDPDTGFSLGRNLPGEIC 423

Query: 427 VRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAP 486
           +RGPQ+MKGYLNDP +T+ T+DVEGWLHTGDIGYVDD++E+FIVDRVKE+IKFKGFQV P
Sbjct: 424 IRGPQIMKGYLNDPEATAATIDVEGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPP 483

Query: 487 AELEALLVTHTSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKR 546
           AELE+LL+ H SI DAAVVPQ DDVAGEVPVAFVV +  + +TEES+KEFI+KQVVFYKR
Sbjct: 484 AELESLLIAHPSIADAAVVPQKDDVAGEVPVAFVVRAADSDITEESIKEFISKQVVFYKR 543

Query: 547 LHKVYFVKTIPKSPSGKTLRKELKAKLS 559
           LHKV+F+  IPKS SGK LR+EL+AKL+
Sbjct: 544 LHKVHFIHAIPKSASGKILRRELRAKLA 567

BLAST of Pay0020002 vs. ExPASy Swiss-Prot
Match: A0A2H5AIY4 (4-coumarate-CoA ligase 2 OS=Narcissus pseudonarcissus OX=39639 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 3.6e-193
Identity = 352/554 (63.54%), Postives = 437/554 (78.88%), Query Frame = 0

Query: 23  SPP----PSTPAT-HVFRSKLPDIPIPDHLPLHSYCFQKLSQVSDRPCLIVGSTGKSYSY 82
           SPP    PSTP T  +FRS+LPDI + +HLPLH Y F+  +  S  PC+I  STG+SYS+
Sbjct: 14  SPPDTTAPSTPPTATIFRSRLPDIVLSNHLPLHEYLFEN-TLTSPSPCIISASTGRSYSF 73

Query: 83  SETHLFSRKAAATF-SKLGVQRGDVIMILLHNSPEFIFSFMGSSMLGAIATTANPYYTGA 142
           +ETHL SRK A+   S+ GV+RG V+M+LLHN PEF+FSF+GSSMLGA+ T ANP+ T  
Sbjct: 74  AETHLLSRKTASFLSSRCGVRRGGVVMLLLHNCPEFVFSFLGSSMLGAVTTAANPFCTPQ 133

Query: 143 EISKQLKASGAKFVVTYSRCVDKLRECGEV--LTIVTVDD--------PPENCLSFSMVY 202
           EI KQL ASGA  ++T S    K+    +V  L +VTV D        PPE C+SFS V 
Sbjct: 134 EIKKQLLASGATVIITQSAYASKISRDDDVEDLIVVTVSDNELERHAAPPEGCVSFSEVE 193

Query: 203 DANENDVP-FVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLKR 262
            A+E+ VP  V +   DAV++PFSSGTTGLPKGV+LTHK+M SSV Q VDGENPNL+L++
Sbjct: 194 SADEDAVPDAVGVGPEDAVAMPFSSGTTGLPKGVVLTHKSMTSSVGQIVDGENPNLHLRK 253

Query: 263 -NDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATVVPPL 322
             D++LCVLP+FHIFSL+S++L  +R+GAA+++M +FE+E +L  ++R  V+VA VVPPL
Sbjct: 254 GEDVLLCVLPLFHIFSLNSVLLCGLRTGAAVVIMARFEMEGMLETIQRWGVSVAAVVPPL 313

Query: 323 VVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSM 382
           V++L KNP V  +++ ++R+VLSGAAPL KELE  L  RLPQA+ GQGYGMTEAGPV+SM
Sbjct: 314 VLALAKNPLVEKYDMGTVRMVLSGAAPLGKELEAVLKGRLPQAVLGQGYGMTEAGPVISM 373

Query: 383 CSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGYLNDP 442
              FAK+ P P KSGSCG VVRN+ELKV+DP TG SL  NQPGEICVRGPQ+MKGYLNDP
Sbjct: 374 SPGFAKQ-PTPVKSGSCGTVVRNAELKVMDPETGFSLGRNQPGEICVRGPQIMKGYLNDP 433

Query: 443 VSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSIV 502
            +TS T+DVEGWLHTGD+GYVDD++E+FIVDRVKE+IKFKGFQV PAELEALL+ H SI 
Sbjct: 434 EATSATIDVEGWLHTGDVGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLLGHPSIA 493

Query: 503 DAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTIPKSP 559
           DAAV+PQND+VAGEVPVAFVVPS  + LTEE VKEFI+KQVVFYKR+H+VYF+  IPKSP
Sbjct: 494 DAAVIPQNDEVAGEVPVAFVVPSKSSDLTEEIVKEFISKQVVFYKRIHRVYFIHAIPKSP 553

BLAST of Pay0020002 vs. ExPASy Swiss-Prot
Match: P41636 (4-coumarate--CoA ligase OS=Pinus taeda OX=3352 GN=4CL PE=2 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 5.7e-191
Identity = 325/529 (61.44%), Postives = 431/529 (81.47%), Query Frame = 0

Query: 32  HVFRSKLPDIPIPDHLPLHSYCFQKLSQVSDRPCLIVGSTGKSYSYSETHLFSRKAAATF 91
           H++RSKLPDI I DHLPLHSYCF+++++ +DRPCLI G+T ++Y +SE  L SRK AA  
Sbjct: 10  HLYRSKLPDIEISDHLPLHSYCFERVAEFADRPCLIDGATDRTYCFSEVELISRKVAAGL 69

Query: 92  SKLGVQRGDVIMILLHNSPEFIFSFMGSSMLGAIATTANPYYTGAEISKQLKASGAKFVV 151
           +KLG+Q+G V+M+LL N  EF F FMG+S+ GAI TTANP+Y   EI+KQ KA+GA+ +V
Sbjct: 70  AKLGLQQGQVVMLLLPNCIEFAFVFMGASVRGAIVTTANPFYKPGEIAKQAKAAGARIIV 129

Query: 152 TYSRCVDKLR--ECGEVLTIVTVDDPPENCLSFSMVYDANENDVPFVEIDTNDAVSLPFS 211
           T +  V+KL   +  +VL I   D P E C   S++ +A+E   P V+I  +D V+LP+S
Sbjct: 130 TLAAYVEKLADLQSHDVLVITIDDAPKEGCQHISVLTEADETQCPAVKIHPDDVVALPYS 189

Query: 212 SGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLKRNDIVLCVLPMFHIFSLSSIVLISI 271
           SGTTGLPKGV+LTHK +VSSVAQQVDGENPNLY   +D++LCVLP+FHI+SL+S++L ++
Sbjct: 190 SGTTGLPKGVMLTHKGLVSSVAQQVDGENPNLYFHSDDVILCVLPLFHIYSLNSVLLCAL 249

Query: 272 RSGAALLLMEKFEIESLLRLVERHKVTVATVVPPLVVSLVKNPKVADFNLSSIRLVLSGA 331
           R+GAA L+M+KF + + L L++++KVTVA +VPP+V+ + K+P V+ +++SS+R+++SGA
Sbjct: 250 RAGAATLIMQKFNLTTCLELIQKYKVTVAPIVPPIVLDITKSPIVSQYDVSSVRIIMSGA 309

Query: 332 APLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPPMPTKSGSCGRVVRNSE 391
           APL KELE+AL ER P+AIFGQGYGMTEAGPVL+M  AFAK  P P KSGSCG VVRN++
Sbjct: 310 APLGKELEDALRERFPKAIFGQGYGMTEAGPVLAMNLAFAKN-PFPVKSGSCGTVVRNAQ 369

Query: 392 LKVVDPLTGASLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGYVDDEE 451
           +K++D  TG SL +NQ GEIC+RGP++MKGY+NDP ST+ T+D EGWLHTGD+ Y+DD+E
Sbjct: 370 IKILDTETGESLPHNQAGEICIRGPEIMKGYINDPESTAATIDEEGWLHTGDVEYIDDDE 429

Query: 452 EIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSIVDAAVVPQNDDVAGEVPVAFVVPSTQ 511
           EIFIVDRVKEIIK+KGFQVAPAELEALLV H SI DAAVVPQ  + AGEVPVAFVV S++
Sbjct: 430 EIFIVDRVKEIIKYKGFQVAPAELEALLVAHPSIADAAVVPQKHEEAGEVPVAFVVKSSE 489

Query: 512 NGLTEESVKEFIAKQVVFYKRLHKVYFVKTIPKSPSGKTLRKELKAKLS 559
             ++E+ +KEF+AKQV+FYK++H+VYFV  IPKSPSGK LRK+L+++L+
Sbjct: 490 --ISEQEIKEFVAKQVIFYKKIHRVYFVDAIPKSPSGKILRKDLRSRLA 535

BLAST of Pay0020002 vs. ExPASy TrEMBL
Match: A0A5A7THZ8 (4-coumarate--CoA ligase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G002030 PE=4 SV=1)

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 558/558 (100.00%), Postives = 558/558 (100.00%), Query Frame = 0

Query: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60
           MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV
Sbjct: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60

Query: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120
           SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS
Sbjct: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120

Query: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL 180
           MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL
Sbjct: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL 180

Query: 181 SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240
           SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN
Sbjct: 181 SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240

Query: 241 LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV 300
           LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV
Sbjct: 241 LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV 300

Query: 301 VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360
           VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP
Sbjct: 301 VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360

Query: 361 VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY 420
           VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY
Sbjct: 361 VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY 420

Query: 421 LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480
           LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH
Sbjct: 421 LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480

Query: 481 TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI 540
           TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI
Sbjct: 481 TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI 540

Query: 541 PKSPSGKTLRKELKAKLS 559
           PKSPSGKTLRKELKAKLS
Sbjct: 541 PKSPSGKTLRKELKAKLS 558

BLAST of Pay0020002 vs. ExPASy TrEMBL
Match: A0A1S3CJB3 (4-coumarate--CoA ligase 2 OS=Cucumis melo OX=3656 GN=LOC103501067 PE=4 SV=1)

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 558/558 (100.00%), Postives = 558/558 (100.00%), Query Frame = 0

Query: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60
           MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV
Sbjct: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60

Query: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120
           SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS
Sbjct: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120

Query: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL 180
           MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL
Sbjct: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL 180

Query: 181 SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240
           SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN
Sbjct: 181 SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240

Query: 241 LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV 300
           LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV
Sbjct: 241 LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV 300

Query: 301 VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360
           VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP
Sbjct: 301 VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360

Query: 361 VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY 420
           VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY
Sbjct: 361 VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY 420

Query: 421 LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480
           LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH
Sbjct: 421 LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480

Query: 481 TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI 540
           TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI
Sbjct: 481 TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI 540

Query: 541 PKSPSGKTLRKELKAKLS 559
           PKSPSGKTLRKELKAKLS
Sbjct: 541 PKSPSGKTLRKELKAKLS 558

BLAST of Pay0020002 vs. ExPASy TrEMBL
Match: A0A0A0LQV1 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G433350 PE=4 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 7.9e-260
Identity = 465/491 (94.70%), Postives = 480/491 (97.76%), Query Frame = 0

Query: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60
           MISLAP+CDPQ PNNV    SSSPPPSTP THVFRSKLPDIPIPDHLPLH+Y FQKLSQV
Sbjct: 1   MISLAPLCDPQQPNNV----SSSPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQV 60

Query: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120
           SDRPCLIVGSTGKSYSYSETHL SRKAAATFSKLGV+RGDVIMILLHNSPEFIFSFMGSS
Sbjct: 61  SDRPCLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSS 120

Query: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRE-CGEVLTIVTVDDPPENC 180
           MLGA+ATTANPYYTGAEIS+QLKASGAKFVVTYSRCVDKLRE CG+VLTIVT+D PPENC
Sbjct: 121 MLGAVATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENC 180

Query: 181 LSFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENP 240
           LSFSMVYDA+ENDVP VEIDTNDAV+LPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENP
Sbjct: 181 LSFSMVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENP 240

Query: 241 NLYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVAT 300
           NLYLKRND+VLCVLPMFHIFSLSSIVLIS+RSGAALLL+EKFEIESLLRLVERHKVTVAT
Sbjct: 241 NLYLKRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVAT 300

Query: 301 VVPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAG 360
           VVPPLVVSLVKNPKVADF+LSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAG
Sbjct: 301 VVPPLVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAG 360

Query: 361 PVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKG 420
           PVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDP+TGASLTYNQPGEICVRGPQVMKG
Sbjct: 361 PVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKG 420

Query: 421 YLNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVT 480
           YLNDPVSTSLTVDVEGWLHTGDIG+VDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVT
Sbjct: 421 YLNDPVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVT 480

Query: 481 HTSIVDAAVVP 491
           HTSIVDAAVVP
Sbjct: 481 HTSIVDAAVVP 487

BLAST of Pay0020002 vs. ExPASy TrEMBL
Match: A0A6J1G8F9 (4-coumarate--CoA ligase 3 OS=Cucurbita moschata OX=3662 GN=LOC111451685 PE=4 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 4.2e-253
Identity = 443/558 (79.39%), Postives = 501/558 (89.78%), Query Frame = 0

Query: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60
           MIS+A + D + P       SSSPPP      VFRSKLPDI IPDHLPLH YCF+K+S+ 
Sbjct: 1   MISVAQLSDGEKPK-----LSSSPPP-----RVFRSKLPDITIPDHLPLHDYCFEKISEF 60

Query: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120
           SDRPCLIVG+TGKSYS+S+THLFS++AAATFSKLGV++GD IMILL NS EF+FSFMGSS
Sbjct: 61  SDRPCLIVGATGKSYSFSDTHLFSKRAAATFSKLGVKKGDFIMILLKNSAEFVFSFMGSS 120

Query: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL 180
           M+G++ATTANPYYT AEISKQLK SGAK VVTYS CVDKLRE    LTIVTVDDPPENCL
Sbjct: 121 MIGSVATTANPYYTAAEISKQLKMSGAKLVVTYSHCVDKLRESDVDLTIVTVDDPPENCL 180

Query: 181 SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240
           SFSMVYDA+ENDVPFVEID NDAVSLPFSSGTTG PKGV+LTHK+MVSS+AQQVDGENPN
Sbjct: 181 SFSMVYDADENDVPFVEIDWNDAVSLPFSSGTTGTPKGVVLTHKSMVSSIAQQVDGENPN 240

Query: 241 LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV 300
           +YL  ND+VLCVLPMFHIFSLSSIVLISIRSGA +LL+EKFEIE+ + L+ERH VTVATV
Sbjct: 241 IYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIEKFEIETSMSLIERHGVTVATV 300

Query: 301 VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360
           VPP+V+ +VKNPKVADFNLSSIR+V+SGAAPL K++EEALMER+PQA+ GQGYGMTEAGP
Sbjct: 301 VPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEALMERIPQAVIGQGYGMTEAGP 360

Query: 361 VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY 420
           VLSMC+AFAKEPPMPTK GSCGRVVRNSELK++DP TG SLTYNQPGEIC+RGPQ+MKGY
Sbjct: 361 VLSMCTAFAKEPPMPTKIGSCGRVVRNSELKIIDPKTGVSLTYNQPGEICIRGPQIMKGY 420

Query: 421 LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480
           LNDPV+TSLTVDVEGWLHTGDIGY+D+EEEIFIVDR+KEIIK+KGFQVAP ELE+LL+TH
Sbjct: 421 LNDPVATSLTVDVEGWLHTGDIGYIDEEEEIFIVDRIKEIIKYKGFQVAPVELESLLLTH 480

Query: 481 TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI 540
            SI++ AVV +ND++AGEVPVAFVV S  N LT+E VKEFIAKQVVFYKRLH+VYFV TI
Sbjct: 481 PSIMEVAVVAENDEIAGEVPVAFVVCSAGNQLTQEEVKEFIAKQVVFYKRLHRVYFVPTI 540

Query: 541 PKSPSGKTLRKELKAKLS 559
           PK PSGK L+K+LKAKLS
Sbjct: 541 PKLPSGKILKKDLKAKLS 548

BLAST of Pay0020002 vs. ExPASy TrEMBL
Match: A0A4Y1R2Z5 (4-coumarate:CoA ligase 3 OS=Prunus dulcis OX=3755 GN=ALMOND_2B015040 PE=4 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 1.5e-221
Identity = 394/564 (69.86%), Postives = 471/564 (83.51%), Query Frame = 0

Query: 12  PPNNVSSDFSSSPPPSTPAT--HVFRSKLPDIPIPDHLPLHSYCFQKLSQVSDRPCLIVG 71
           P NN+ S  +++   +T  T  HVF+SKLPDIPIPDHLPLH+YCFQKLS+ SDRPCLIVG
Sbjct: 38  PDNNIHSPTTAAAATTTSNTTNHVFKSKLPDIPIPDHLPLHTYCFQKLSEFSDRPCLIVG 97

Query: 72  STGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSSMLGAIATTA 131
           STGKSYS+S+T+L S+K  A  S LG+++GDVIMILL N  EF+F+FMG+SM+GA+ TTA
Sbjct: 98  STGKSYSFSDTYLISQKTGAGLSNLGIKKGDVIMILLQNCAEFVFAFMGASMIGAVTTTA 157

Query: 132 NPYYTGAEISKQLKASGAKFVVTYSRCVDKLREC---------------GEVLTIVTVDD 191
           NP+YT AEI KQ KAS AK ++T+S+ VDKLRE                GE   +VTVDD
Sbjct: 158 NPFYTAAEIFKQFKASNAKLIITHSQFVDKLREAPADGSAPYQNNYPKLGEHFKVVTVDD 217

Query: 192 PPENCLSFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQV 251
           PPENCL FS++ +ANE ++P V ID  D V+LPFSSGTTGLPKGVILTHK++++SVAQQV
Sbjct: 218 PPENCLHFSVISEANETELPEVVIDPEDPVALPFSSGTTGLPKGVILTHKSLITSVAQQV 277

Query: 252 DGENPNLYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHK 311
           DGENPNLYLK +D+VLCVLP+FHI+SL+S++L S+R+GAA+L+M KFEI +LL L++R++
Sbjct: 278 DGENPNLYLKEDDVVLCVLPLFHIYSLNSVMLCSLRAGAAVLVMHKFEIGTLLELIQRYR 337

Query: 312 VTVATVVPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYG 371
           V+VA VVPPLV++L KNP VA F+LSSIR+VLSGAAPL KELEEAL  R+PQA+ GQGYG
Sbjct: 338 VSVAAVVPPLVIALAKNPMVAQFDLSSIRVVLSGAAPLGKELEEALKSRVPQAVLGQGYG 397

Query: 372 MTEAGPVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGP 431
           MTEAGPVLSMC AFAKE P+PTKSGSCG VVRN+ELKV+D  TG SL YNQPGEIC+RG 
Sbjct: 398 MTEAGPVLSMCLAFAKE-PLPTKSGSCGTVVRNAELKVIDAETGCSLGYNQPGEICIRGS 457

Query: 432 QVMKGYLNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELE 491
           Q+MKGYLND  +T+ TVD EGWLHTGD+GYVDD+EE+FIVDRVKE+IKFKGFQV PAELE
Sbjct: 458 QIMKGYLNDAEATATTVDKEGWLHTGDVGYVDDDEEVFIVDRVKELIKFKGFQVPPAELE 517

Query: 492 ALLVTHTSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKV 551
           +LLV+H SI DAAVVPQ D+ AGEVPVAFVV S    LTEE+VKEFIAKQVVFYKRLHKV
Sbjct: 518 SLLVSHPSIADAAVVPQKDEAAGEVPVAFVVRSNGLQLTEEAVKEFIAKQVVFYKRLHKV 577

Query: 552 YFVKTIPKSPSGKTLRKELKAKLS 559
           YFV  IPKSPSGK LRK+L+AKL+
Sbjct: 578 YFVHAIPKSPSGKILRKDLRAKLA 600

BLAST of Pay0020002 vs. NCBI nr
Match: XP_008462779.1 (PREDICTED: 4-coumarate--CoA ligase 2 [Cucumis melo] >KAA0042418.1 4-coumarate--CoA ligase 2 [Cucumis melo var. makuwa] >TYK01002.1 4-coumarate--CoA ligase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 558/558 (100.00%), Postives = 558/558 (100.00%), Query Frame = 0

Query: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60
           MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV
Sbjct: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60

Query: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120
           SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS
Sbjct: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120

Query: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL 180
           MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL
Sbjct: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL 180

Query: 181 SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240
           SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN
Sbjct: 181 SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240

Query: 241 LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV 300
           LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV
Sbjct: 241 LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV 300

Query: 301 VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360
           VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP
Sbjct: 301 VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360

Query: 361 VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY 420
           VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY
Sbjct: 361 VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY 420

Query: 421 LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480
           LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH
Sbjct: 421 LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480

Query: 481 TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI 540
           TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI
Sbjct: 481 TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI 540

Query: 541 PKSPSGKTLRKELKAKLS 559
           PKSPSGKTLRKELKAKLS
Sbjct: 541 PKSPSGKTLRKELKAKLS 558

BLAST of Pay0020002 vs. NCBI nr
Match: XP_004151908.1 (4-coumarate--CoA ligase 2 [Cucumis sativus] >KAE8652343.1 hypothetical protein Csa_021810 [Cucumis sativus])

HSP 1 Score: 1024.6 bits (2648), Expect = 3.1e-295
Identity = 528/559 (94.45%), Postives = 545/559 (97.50%), Query Frame = 0

Query: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60
           MISLAP+CDPQ PNNV    SSSPPPSTP THVFRSKLPDIPIPDHLPLH+Y FQKLSQV
Sbjct: 1   MISLAPLCDPQQPNNV----SSSPPPSTPPTHVFRSKLPDIPIPDHLPLHTYSFQKLSQV 60

Query: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120
           SDRPCLIVGSTGKSYSYSETHL SRKAAATFSKLGV+RGDVIMILLHNSPEFIFSFMGSS
Sbjct: 61  SDRPCLIVGSTGKSYSYSETHLLSRKAAATFSKLGVKRGDVIMILLHNSPEFIFSFMGSS 120

Query: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRE-CGEVLTIVTVDDPPENC 180
           MLGA+ATTANPYYTGAEIS+QLKASGAKFVVTYSRCVDKLRE CG+VLTIVT+D PPENC
Sbjct: 121 MLGAVATTANPYYTGAEISRQLKASGAKFVVTYSRCVDKLRESCGDVLTIVTIDAPPENC 180

Query: 181 LSFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENP 240
           LSFSMVYDA+ENDVP VEIDTNDAV+LPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENP
Sbjct: 181 LSFSMVYDADENDVPSVEIDTNDAVALPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENP 240

Query: 241 NLYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVAT 300
           NLYLKRND+VLCVLPMFHIFSLSSIVLIS+RSGAALLL+EKFEIESLLRLVERHKVTVAT
Sbjct: 241 NLYLKRNDVVLCVLPMFHIFSLSSIVLISMRSGAALLLIEKFEIESLLRLVERHKVTVAT 300

Query: 301 VVPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAG 360
           VVPPLVVSLVKNPKVADF+LSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAG
Sbjct: 301 VVPPLVVSLVKNPKVADFDLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAG 360

Query: 361 PVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKG 420
           PVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDP+TGASLTYNQPGEICVRGPQVMKG
Sbjct: 361 PVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPITGASLTYNQPGEICVRGPQVMKG 420

Query: 421 YLNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVT 480
           YLNDPVSTSLTVDVEGWLHTGDIG+VDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVT
Sbjct: 421 YLNDPVSTSLTVDVEGWLHTGDIGFVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVT 480

Query: 481 HTSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKT 540
           HTSIVDAAVVPQNDDVAGEVPVAF+VPST N L+EESVKEFIAKQVVFYKRLHKVYFVKT
Sbjct: 481 HTSIVDAAVVPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVFYKRLHKVYFVKT 540

Query: 541 IPKSPSGKTLRKELKAKLS 559
           IPKSPSGK LRKELKAKLS
Sbjct: 541 IPKSPSGKILRKELKAKLS 555

BLAST of Pay0020002 vs. NCBI nr
Match: XP_038901546.1 (4-coumarate--CoA ligase 3 isoform X1 [Benincasa hispida])

HSP 1 Score: 976.9 bits (2524), Expect = 7.5e-281
Identity = 500/558 (89.61%), Postives = 530/558 (94.98%), Query Frame = 0

Query: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60
           MIS+A +C+ Q P NVSS+ SSSPPP+   TH+FRSKLPDI IPDHLPLHSYCFQKLS+V
Sbjct: 1   MISVASLCEAQQP-NVSSELSSSPPPAMAPTHIFRSKLPDITIPDHLPLHSYCFQKLSEV 60

Query: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120
            D PCLIVGSTGKSYSYSETHLFSRKAAATFSKLGV++GDVIMILL NSPEFIFSFMGSS
Sbjct: 61  KDLPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVKKGDVIMILLQNSPEFIFSFMGSS 120

Query: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL 180
           MLG +ATTANPYYT AEISKQL ASGAKFVVTYS+CV KLRE GE LTIVTVDDPPENCL
Sbjct: 121 MLGTVATTANPYYTAAEISKQLMASGAKFVVTYSQCVAKLRESGEDLTIVTVDDPPENCL 180

Query: 181 SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240
           SFSMVYDA+ENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN
Sbjct: 181 SFSMVYDADENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240

Query: 241 LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV 300
           LYL+RNDIVLCVLPMFHIFSLSSIVL+SIRSGAALLLMEKFEIESLLRL+E+H VTVATV
Sbjct: 241 LYLRRNDIVLCVLPMFHIFSLSSIVLVSIRSGAALLLMEKFEIESLLRLIEKHGVTVATV 300

Query: 301 VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360
           VPPLVV+LVKNP+ ADF+LSSIR+VLSGAAPLRKELEEALM+RLPQAIFGQGYGMTEAGP
Sbjct: 301 VPPLVVALVKNPRAADFDLSSIRMVLSGAAPLRKELEEALMQRLPQAIFGQGYGMTEAGP 360

Query: 361 VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY 420
           VLSMCSAFAKEPPMPTKSGSCGRVVRNS+LKV+DP TGASL+YNQ GEIC+RGPQVMKGY
Sbjct: 361 VLSMCSAFAKEPPMPTKSGSCGRVVRNSDLKVIDPQTGASLSYNQSGEICLRGPQVMKGY 420

Query: 421 LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480
           LNDPVSTSLTVDVEGWLHTGDIGY+DDE+EIFIVDRVKEIIKFKGFQVAPAELE LLVTH
Sbjct: 421 LNDPVSTSLTVDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELETLLVTH 480

Query: 481 TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI 540
            SIVDAAVVPQNDDVAGEVPVAFVVPST N LTE++VKEFIAKQVVFYKRL KVYFV+TI
Sbjct: 481 PSIVDAAVVPQNDDVAGEVPVAFVVPSTHNELTEDAVKEFIAKQVVFYKRLQKVYFVQTI 540

Query: 541 PKSPSGKTLRKELKAKLS 559
           PKSPSGK LRKELKAKLS
Sbjct: 541 PKSPSGKILRKELKAKLS 557

BLAST of Pay0020002 vs. NCBI nr
Match: XP_038901547.1 (4-coumarate--CoA ligase 3 isoform X2 [Benincasa hispida])

HSP 1 Score: 943.3 bits (2437), Expect = 9.2e-271
Identity = 488/558 (87.46%), Postives = 518/558 (92.83%), Query Frame = 0

Query: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60
           MIS+A +C+ Q P NVSS+ SSSPPP+   TH+FRSKLPDI IPDHLPLHSYCFQKLS+V
Sbjct: 1   MISVASLCEAQQP-NVSSELSSSPPPAMAPTHIFRSKLPDITIPDHLPLHSYCFQKLSEV 60

Query: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120
            D PCLIVGSTGKSYSYSETHLFSRKAAATFSKLGV++GDVIMILL NSPEFIFSFMGSS
Sbjct: 61  KDLPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVKKGDVIMILLQNSPEFIFSFMGSS 120

Query: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL 180
           MLG +ATTANPYYT AEISKQL ASGAKFVVTYS+CV KLRE GE LTIVTVDDPPENCL
Sbjct: 121 MLGTVATTANPYYTAAEISKQLMASGAKFVVTYSQCVAKLRESGEDLTIVTVDDPPENCL 180

Query: 181 SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240
           SFSMVYDA+ENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN
Sbjct: 181 SFSMVYDADENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240

Query: 241 LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV 300
           LYL+RNDIVLCVLPMFHIFSLSSIVL+SIRSGAALLLMEKFEIESLLRL+E+H VTVATV
Sbjct: 241 LYLRRNDIVLCVLPMFHIFSLSSIVLVSIRSGAALLLMEKFEIESLLRLIEKHGVTVATV 300

Query: 301 VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360
           VPPLVV+LVKNP+ ADF+LSSIR+VLSGAAPLRKELEEALM+RLPQAIFGQGYGMTEAGP
Sbjct: 301 VPPLVVALVKNPRAADFDLSSIRMVLSGAAPLRKELEEALMQRLPQAIFGQGYGMTEAGP 360

Query: 361 VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY 420
           VLSMCSAFAKEPPMPTKSGSCGRVVRNS+LKV+DP TGASL+YNQ GEIC+RGPQVMK  
Sbjct: 361 VLSMCSAFAKEPPMPTKSGSCGRVVRNSDLKVIDPQTGASLSYNQSGEICLRGPQVMK-- 420

Query: 421 LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480
                     VDVEGWLHTGDIGY+DDE+EIFIVDRVKEIIKFKGFQVAPAELE LLVTH
Sbjct: 421 ----------VDVEGWLHTGDIGYIDDEDEIFIVDRVKEIIKFKGFQVAPAELETLLVTH 480

Query: 481 TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI 540
            SIVDAAVVPQNDDVAGEVPVAFVVPST N LTE++VKEFIAKQVVFYKRL KVYFV+TI
Sbjct: 481 PSIVDAAVVPQNDDVAGEVPVAFVVPSTHNELTEDAVKEFIAKQVVFYKRLQKVYFVQTI 540

Query: 541 PKSPSGKTLRKELKAKLS 559
           PKSPSGK LRKELKAKLS
Sbjct: 541 PKSPSGKILRKELKAKLS 545

BLAST of Pay0020002 vs. NCBI nr
Match: XP_022947959.1 (4-coumarate--CoA ligase 3 [Cucurbita moschata])

HSP 1 Score: 883.6 bits (2282), Expect = 8.7e-253
Identity = 443/558 (79.39%), Postives = 501/558 (89.78%), Query Frame = 0

Query: 1   MISLAPVCDPQPPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQV 60
           MIS+A + D + P       SSSPPP      VFRSKLPDI IPDHLPLH YCF+K+S+ 
Sbjct: 1   MISVAQLSDGEKPK-----LSSSPPP-----RVFRSKLPDITIPDHLPLHDYCFEKISEF 60

Query: 61  SDRPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSS 120
           SDRPCLIVG+TGKSYS+S+THLFS++AAATFSKLGV++GD IMILL NS EF+FSFMGSS
Sbjct: 61  SDRPCLIVGATGKSYSFSDTHLFSKRAAATFSKLGVKKGDFIMILLKNSAEFVFSFMGSS 120

Query: 121 MLGAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDPPENCL 180
           M+G++ATTANPYYT AEISKQLK SGAK VVTYS CVDKLRE    LTIVTVDDPPENCL
Sbjct: 121 MIGSVATTANPYYTAAEISKQLKMSGAKLVVTYSHCVDKLRESDVDLTIVTVDDPPENCL 180

Query: 181 SFSMVYDANENDVPFVEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPN 240
           SFSMVYDA+ENDVPFVEID NDAVSLPFSSGTTG PKGV+LTHK+MVSS+AQQVDGENPN
Sbjct: 181 SFSMVYDADENDVPFVEIDWNDAVSLPFSSGTTGTPKGVVLTHKSMVSSIAQQVDGENPN 240

Query: 241 LYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATV 300
           +YL  ND+VLCVLPMFHIFSLSSIVLISIRSGA +LL+EKFEIE+ + L+ERH VTVATV
Sbjct: 241 IYLGTNDVVLCVLPMFHIFSLSSIVLISIRSGATILLIEKFEIETSMSLIERHGVTVATV 300

Query: 301 VPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGP 360
           VPP+V+ +VKNPKVADFNLSSIR+V+SGAAPL K++EEALMER+PQA+ GQGYGMTEAGP
Sbjct: 301 VPPMVLMMVKNPKVADFNLSSIRMVISGAAPLGKQIEEALMERIPQAVIGQGYGMTEAGP 360

Query: 361 VLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGY 420
           VLSMC+AFAKEPPMPTK GSCGRVVRNSELK++DP TG SLTYNQPGEIC+RGPQ+MKGY
Sbjct: 361 VLSMCTAFAKEPPMPTKIGSCGRVVRNSELKIIDPKTGVSLTYNQPGEICIRGPQIMKGY 420

Query: 421 LNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTH 480
           LNDPV+TSLTVDVEGWLHTGDIGY+D+EEEIFIVDR+KEIIK+KGFQVAP ELE+LL+TH
Sbjct: 421 LNDPVATSLTVDVEGWLHTGDIGYIDEEEEIFIVDRIKEIIKYKGFQVAPVELESLLLTH 480

Query: 481 TSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTI 540
            SI++ AVV +ND++AGEVPVAFVV S  N LT+E VKEFIAKQVVFYKRLH+VYFV TI
Sbjct: 481 PSIMEVAVVAENDEIAGEVPVAFVVCSAGNQLTQEEVKEFIAKQVVFYKRLHRVYFVPTI 540

Query: 541 PKSPSGKTLRKELKAKLS 559
           PK PSGK L+K+LKAKLS
Sbjct: 541 PKLPSGKILKKDLKAKLS 548

BLAST of Pay0020002 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 744.6 bits (1921), Expect = 5.9e-215
Identity = 378/559 (67.62%), Postives = 463/559 (82.83%), Query Frame = 0

Query: 5   APVCDPQ--PPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQVSD 64
           A + +PQ   P + S       P S P   +FRSKLPDI IP+HLPLH+YCF+KLS VSD
Sbjct: 4   AALHEPQIHKPTDTSVVSDDVLPHSPPTPRIFRSKLPDIDIPNHLPLHTYCFEKLSSVSD 63

Query: 65  RPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSSML 124
           +PCLIVGSTGKSY+Y ETHL  R+ A+   KLG+++GDVIMILL NS EF+FSFMG+SM+
Sbjct: 64  KPCLIVGSTGKSYTYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMI 123

Query: 125 GAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDP-PENCLS 184
           GA++TTANP+YT  E+ KQLK+SGAK ++T+S+ VDKL+  GE LT++T D+P PENCL 
Sbjct: 124 GAVSTTANPFYTSQELYKQLKSSGAKLIITHSQYVDKLKNLGENLTLITTDEPTPENCLP 183

Query: 185 FSMVYDANENDVPF---VEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGEN 244
           FS +   +E + PF   V+I  +DA +LPFSSGTTGLPKGV+LTHK++++SVAQQVDG+N
Sbjct: 184 FSTLITDDETN-PFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDN 243

Query: 245 PNLYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVA 304
           PNLYLK ND++LCVLP+FHI+SL+S++L S+RSGA +LLM KFEI +LL L++RH+VT+A
Sbjct: 244 PNLYLKSNDVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIA 303

Query: 305 TVVPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEA 364
            +VPPLV++L KNP V  ++LSS+R VLSGAAPL KEL+++L  RLPQAI GQGYGMTEA
Sbjct: 304 ALVPPLVIALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEA 363

Query: 365 GPVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMK 424
           GPVLSM   FAKE P+PTKSGSCG VVRN+ELKVV   T  SL YNQPGEIC+RG Q+MK
Sbjct: 364 GPVLSMSLGFAKE-PIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMK 423

Query: 425 GYLNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLV 484
            YLNDP +TS T+D EGWLHTGDIGYVD+++EIFIVDR+KE+IKFKGFQV PAELE+LL+
Sbjct: 424 EYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLI 483

Query: 485 THTSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVK 544
            H SI DAAVVPQND+VAGEVPVAFVV S  N +TEE VKE++AKQVVFYKRLHKV+FV 
Sbjct: 484 NHHSIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVA 543

Query: 545 TIPKSPSGKTLRKELKAKL 558
           +IPKSPSGK LRK+LKAKL
Sbjct: 544 SIPKSPSGKILRKDLKAKL 560

BLAST of Pay0020002 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 634.8 bits (1636), Expect = 6.5e-182
Identity = 314/531 (59.13%), Postives = 420/531 (79.10%), Query Frame = 0

Query: 33  VFRSKLPDIPIPDHLPLHSYCFQKLSQVSDRPCLIVGSTGKSYSYSETHLFSRKAAATFS 92
           +FRS+LPDI IP+HLPLH Y F+ +S+ + +PCLI G TG+ Y+Y++ H+ SRK AA   
Sbjct: 22  IFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAGLH 81

Query: 93  KLGVQRGDVIMILLHNSPEFIFSFMGSSMLGAIATTANPYYTGAEISKQLKASGAKFVVT 152
            LGV++ DV+MILL NSPE + +F+ +S +GAI T+ANP++T AEISKQ KAS AK +VT
Sbjct: 82  NLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVT 141

Query: 153 YSRCVDKLREC-GEVLTIVTVDDP--PENCLSFSMVYDANENDVPFV--EIDTNDAVSLP 212
            SR VDK++    + + IVT D    PENCL FS +  + E  V  +  +I   D V+LP
Sbjct: 142 QSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDVVALP 201

Query: 213 FSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLKRNDIVLCVLPMFHIFSLSSIVLI 272
           FSSGTTGLPKGV+LTHK +V+SVAQQVDGENPNLY  R+D++LCVLPMFHI++L+SI+L 
Sbjct: 202 FSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSIMLC 261

Query: 273 SIRSGAALLLMEKFEIESLLRLVERHKVTVATVVPPLVVSLVKNPKVADFNLSSIRLVLS 332
           S+R GA +L+M KFEI  LL  ++R KVTVA VVPP+V+++ K+P+   ++LSS+R+V S
Sbjct: 262 SLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRMVKS 321

Query: 333 GAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPPMPTKSGSCGRVVRN 392
           GAAPL KELE+A+  + P A  GQGYGMTEAGPVL+M   FAKE P P KSG+CG VVRN
Sbjct: 322 GAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKE-PFPVKSGACGTVVRN 381

Query: 393 SELKVVDPLTGASLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDIGYVDD 452
           +E+K++DP TG SL  N+PGEIC+RG Q+MKGYLNDP++T+ T+D +GWLHTGD+G++DD
Sbjct: 382 AEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFIDD 441

Query: 453 EEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSIVDAAVVPQNDDVAGEVPVAFVVPS 512
           ++E+FIVDR+KE+IK+KGFQVAPAELE+LL+ H  I D AVV   ++ AGEVPVAFVV S
Sbjct: 442 DDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVVRS 501

Query: 513 TQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTIPKSPSGKTLRKELKAKLS 559
             + ++E+ +K+F++KQVVFYKR++KV+F  +IPK+PSGK LRK+L+A+L+
Sbjct: 502 KDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLA 551

BLAST of Pay0020002 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 629.0 bits (1621), Expect = 3.6e-180
Identity = 315/536 (58.77%), Postives = 416/536 (77.61%), Query Frame = 0

Query: 33  VFRSKLPDIPIPDHLPLHSYCFQKLSQVSDRPCLIVGSTGKSYSYSETHLFSRKAAATFS 92
           +FRSKLPDI IP+HL LH Y FQ +S+ + +PCLI G TG  Y+YS+ H+ SR+ AA F 
Sbjct: 24  IFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFH 83

Query: 93  KLGVQRGDVIMILLHNSPEFIFSFMGSSMLGAIATTANPYYTGAEISKQLKASGAKFVVT 152
           KLGV + DV+M+LL N PEF+ SF+ +S  GA AT ANP++T AEI+KQ KAS  K ++T
Sbjct: 84  KLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIIT 143

Query: 153 YSRCVDKLR--ECGEVLTIVTVDDP-----PENCLSFSMVYDAN---ENDVPFVEIDTND 212
            +R VDK++  +  + + IV +DD      PE CL F+ +  +       +  VEI  +D
Sbjct: 144 EARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDD 203

Query: 213 AVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLKRNDIVLCVLPMFHIFSLS 272
            V+LP+SSGTTGLPKGV+LTHK +V+SVAQQVDGENPNLY   +D++LCVLPMFHI++L+
Sbjct: 204 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALN 263

Query: 273 SIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATVVPPLVVSLVKNPKVADFNLSSI 332
           SI+L  +R GAA+L+M KFEI  LL L++R KVTVA +VPP+V+++ K+ +   ++LSSI
Sbjct: 264 SIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSI 323

Query: 333 RLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPPMPTKSGSCG 392
           R+V SGAAPL KELE+A+  + P A  GQGYGMTEAGPVL+M   FAKE P P KSG+CG
Sbjct: 324 RVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKE-PFPVKSGACG 383

Query: 393 RVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDI 452
            VVRN+E+K+VDP TG SL+ NQPGEIC+RG Q+MKGYLN+P +T+ T+D +GWLHTGDI
Sbjct: 384 TVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGDI 443

Query: 453 GYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSIVDAAVVPQNDDVAGEVPVA 512
           G +DD++E+FIVDR+KE+IK+KGFQVAPAELEALL+ H  I D AVV   ++ AGEVPVA
Sbjct: 444 GLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPVA 503

Query: 513 FVVPSTQNGLTEESVKEFIAKQVVFYKRLHKVYFVKTIPKSPSGKTLRKELKAKLS 559
           FVV S  + L+E+ VK+F++KQVVFYKR++KV+F ++IPK+PSGK LRK+L+AKL+
Sbjct: 504 FVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 558

BLAST of Pay0020002 vs. TAIR 10
Match: AT1G65060.2 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 615.9 bits (1587), Expect = 3.1e-176
Identity = 309/472 (65.47%), Postives = 386/472 (81.78%), Query Frame = 0

Query: 5   APVCDPQ--PPNNVSSDFSSSPPPSTPATHVFRSKLPDIPIPDHLPLHSYCFQKLSQVSD 64
           A + +PQ   P + S       P S P   +FRSKLPDI IP+HLPLH+YCF+KLS VSD
Sbjct: 4   AALHEPQIHKPTDTSVVSDDVLPHSPPTPRIFRSKLPDIDIPNHLPLHTYCFEKLSSVSD 63

Query: 65  RPCLIVGSTGKSYSYSETHLFSRKAAATFSKLGVQRGDVIMILLHNSPEFIFSFMGSSML 124
           +PCLIVGSTGKSY+Y ETHL  R+ A+   KLG+++GDVIMILL NS EF+FSFMG+SM+
Sbjct: 64  KPCLIVGSTGKSYTYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMI 123

Query: 125 GAIATTANPYYTGAEISKQLKASGAKFVVTYSRCVDKLRECGEVLTIVTVDDP-PENCLS 184
           GA++TTANP+YT  E+ KQLK+SGAK ++T+S+ VDKL+  GE LT++T D+P PENCL 
Sbjct: 124 GAVSTTANPFYTSQELYKQLKSSGAKLIITHSQYVDKLKNLGENLTLITTDEPTPENCLP 183

Query: 185 FSMVYDANENDVPF---VEIDTNDAVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGEN 244
           FS +   +E + PF   V+I  +DA +LPFSSGTTGLPKGV+LTHK++++SVAQQVDG+N
Sbjct: 184 FSTLITDDETN-PFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDN 243

Query: 245 PNLYLKRNDIVLCVLPMFHIFSLSSIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVA 304
           PNLYLK ND++LCVLP+FHI+SL+S++L S+RSGA +LLM KFEI +LL L++RH+VT+A
Sbjct: 244 PNLYLKSNDVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIA 303

Query: 305 TVVPPLVVSLVKNPKVADFNLSSIRLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEA 364
            +VPPLV++L KNP V  ++LSS+R VLSGAAPL KEL+++L  RLPQAI GQGYGMTEA
Sbjct: 304 ALVPPLVIALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEA 363

Query: 365 GPVLSMCSAFAKEPPMPTKSGSCGRVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMK 424
           GPVLSM   FAKE P+PTKSGSCG VVRN+ELKVV   T  SL YNQPGEIC+RG Q+MK
Sbjct: 364 GPVLSMSLGFAKE-PIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMK 423

Query: 425 GYLNDPVSTSLTVDVEGWLHTGDIGYVDDEEEIFIVDRVKEIIKFKGFQVAP 471
            YLNDP +TS T+D EGWLHTGDIGYVD+++EIFIVDR+KE+IKFKGFQ +P
Sbjct: 424 EYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQASP 473

BLAST of Pay0020002 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 583.6 bits (1503), Expect = 1.7e-166
Identity = 293/503 (58.25%), Postives = 385/503 (76.54%), Query Frame = 0

Query: 33  VFRSKLPDIPIPDHLPLHSYCFQKLSQVSDRPCLIVGSTGKSYSYSETHLFSRKAAATFS 92
           +FRSKLPDI IP+HL LH Y FQ +S+ + +PCLI G TG  Y+YS+ H+ SR+ AA F 
Sbjct: 24  IFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFH 83

Query: 93  KLGVQRGDVIMILLHNSPEFIFSFMGSSMLGAIATTANPYYTGAEISKQLKASGAKFVVT 152
           KLGV + DV+M+LL N PEF+ SF+ +S  GA AT ANP++T AEI+KQ KAS  K ++T
Sbjct: 84  KLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIIT 143

Query: 153 YSRCVDKLR--ECGEVLTIVTVDDP-----PENCLSFSMVYDAN---ENDVPFVEIDTND 212
            +R VDK++  +  + + IV +DD      PE CL F+ +  +       +  VEI  +D
Sbjct: 144 EARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDD 203

Query: 213 AVSLPFSSGTTGLPKGVILTHKNMVSSVAQQVDGENPNLYLKRNDIVLCVLPMFHIFSLS 272
            V+LP+SSGTTGLPKGV+LTHK +V+SVAQQVDGENPNLY   +D++LCVLPMFHI++L+
Sbjct: 204 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALN 263

Query: 273 SIVLISIRSGAALLLMEKFEIESLLRLVERHKVTVATVVPPLVVSLVKNPKVADFNLSSI 332
           SI+L  +R GAA+L+M KFEI  LL L++R KVTVA +VPP+V+++ K+ +   ++LSSI
Sbjct: 264 SIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSI 323

Query: 333 RLVLSGAAPLRKELEEALMERLPQAIFGQGYGMTEAGPVLSMCSAFAKEPPMPTKSGSCG 392
           R+V SGAAPL KELE+A+  + P A  GQGYGMTEAGPVL+M   FAKE P P KSG+CG
Sbjct: 324 RVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKE-PFPVKSGACG 383

Query: 393 RVVRNSELKVVDPLTGASLTYNQPGEICVRGPQVMKGYLNDPVSTSLTVDVEGWLHTGDI 452
            VVRN+E+K+VDP TG SL+ NQPGEIC+RG Q+MKGYLN+P +T+ T+D +GWLHTGDI
Sbjct: 384 TVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGDI 443

Query: 453 GYVDDEEEIFIVDRVKEIIKFKGFQVAPAELEALLVTHTSIVDAAVVPQNDDVAGEVPVA 512
           G +DD++E+FIVDR+KE+IK+KGFQVAPAELEALL+ H  I D AVV   ++ AGEVPVA
Sbjct: 444 GLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPVA 503

Query: 513 FVVPSTQNGLTEESVKEFIAKQV 526
           FVV S  + L+E+ VK+F++KQV
Sbjct: 504 FVVKSKDSELSEDDVKQFVSKQV 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P316876.7e-21668.744-coumarate--CoA ligase 2 OS=Glycine max OX=3847 PE=2 SV=2[more]
Q9S7778.2e-21467.624-coumarate--CoA ligase 3 OS=Arabidopsis thaliana OX=3702 GN=4CL3 PE=1 SV=1[more]
Q429822.0e-19662.684-coumarate--CoA ligase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CL2 PE=1 ... [more]
A0A2H5AIY43.6e-19363.544-coumarate-CoA ligase 2 OS=Narcissus pseudonarcissus OX=39639 GN=4CL2 PE=2 SV=1[more]
P416365.7e-19161.444-coumarate--CoA ligase OS=Pinus taeda OX=3352 GN=4CL PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7THZ80.0e+00100.004-coumarate--CoA ligase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3CJB30.0e+00100.004-coumarate--CoA ligase 2 OS=Cucumis melo OX=3656 GN=LOC103501067 PE=4 SV=1[more]
A0A0A0LQV17.9e-26094.70AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G433350... [more]
A0A6J1G8F94.2e-25379.394-coumarate--CoA ligase 3 OS=Cucurbita moschata OX=3662 GN=LOC111451685 PE=4 SV=... [more]
A0A4Y1R2Z51.5e-22169.864-coumarate:CoA ligase 3 OS=Prunus dulcis OX=3755 GN=ALMOND_2B015040 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_008462779.10.0e+00100.00PREDICTED: 4-coumarate--CoA ligase 2 [Cucumis melo] >KAA0042418.1 4-coumarate--C... [more]
XP_004151908.13.1e-29594.454-coumarate--CoA ligase 2 [Cucumis sativus] >KAE8652343.1 hypothetical protein C... [more]
XP_038901546.17.5e-28189.614-coumarate--CoA ligase 3 isoform X1 [Benincasa hispida][more]
XP_038901547.19.2e-27187.464-coumarate--CoA ligase 3 isoform X2 [Benincasa hispida][more]
XP_022947959.18.7e-25379.394-coumarate--CoA ligase 3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G65060.15.9e-21567.624-coumarate:CoA ligase 3 [more]
AT3G21240.16.5e-18259.134-coumarate:CoA ligase 2 [more]
AT1G51680.13.6e-18058.774-coumarate:CoA ligase 1 [more]
AT1G65060.23.1e-17665.474-coumarate:CoA ligase 3 [more]
AT1G51680.31.7e-16658.254-coumarate:CoA ligase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 27..457
e-value: 3.2E-152
score: 509.2
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 58..463
e-value: 6.6E-105
score: 351.1
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 472..547
e-value: 5.8E-16
score: 59.2
NoneNo IPR availableGENE3D3.30.300.30coord: 458..558
e-value: 4.7E-28
score: 99.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..29
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availablePANTHERPTHR24096:SF1694-COUMARATE--COA LIGASE 3coord: 32..556
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 32..556
NoneNo IPR availableCDDcd059044CLcoord: 43..553
e-value: 0.0
score: 677.416
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 29..556
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 206..217

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0020002.1Pay0020002.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009698 phenylpropanoid metabolic process
biological_process GO:0010584 pollen exine formation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016207 4-coumarate-CoA ligase activity
molecular_function GO:0106290 trans-cinnamate-CoA ligase activity