Pay0018781 (gene) Melon (Payzawat) v1

Overview
NamePay0018781
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionProtein ASPARTIC PROTEASE IN GUARD CELL 1-like
Locationchr02: 21949211 .. 21950986 (-)
RNA-Seq ExpressionPay0018781
SyntenyPay0018781
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCAATAATTCATTATCTTTTTCATTCCAACATGAAGACTTCACTTTCCTCTGTTTTTCTCTTCCTAACAATCTTCACTTCCCTTCAATTTTCTTCCATTCTCTCTCGCAAGTTAACACAATCACCTTATTCCACTTCCATCTTCGATGTCTTGGCCTCCACAAACCAAGCCCTAAATGCCCTCTCCATTAAACCTAAACATCTTCAAACTCACTCTCACCTTCCAAATTCCTCTTTATCTCTGCCATTGTACCCTAGATTGTCCCTTCATAACCCTTCTTACAAGGACTACGATAGTCTTGTTAGGGCTCGACTCGCTCGTGATGCCGCTCGAGTTCAATTCCTTAACCGAAATCTTGAGCACTCTTTAAATGGGGGTAAGGATTTTGGTGAAGTTACTAATGGGTCTCTAATTGGAGATTCAATTACTGCTCCAGTTGTTTCAGGGCAAAGTAAAGGGAGTGGTGCGGAGTATTTAGCTCAGGTTGGCGTTGGTCAGCCTGTGAAGTTGTTTTATTTGGTGCCTGATACTGGCAGTGATGTCACGTGGCTTCAATGTCAGCCTTGTGCTACTGAGAACGCTTGTTATAAACAAATTGACCCGATTTTCGACCCGAAATCATCGTCTTCTTACACTCCTTTGTCTTGCAATTCTCAACAATGTGGGTTGCTAGATAGACCTAATTGCAATTCCGGCACATGTATATACCAAGTCCACTATGGTGATGGATCATTCACAACTGGTGAACTTGCCACCGAAATACTATCGTTCGGAAATTCAAATTCTATCCCTAATCTCCCAATTGGTTGTGGCCATGACAATGAAGGCTTGTTCGCTGGAGGTGCTGGTTTAATAGGTCTCGGTGGTGGTGCCATTTCCCTTTCTTCCCAATTAAAAGCGTCATCATTTTCATATTGCCTTGTCAACATGGATTCAGACTCATCCTCCACTCTTGAGTTCAACTCAAACATGCCCAGTGACTCACTAACCTCTCCACTTGTGAAAAACGATCGATTTCACTCATATAGGTACGTCAAAGTCGTTGGAATAAGTGTTGGGGGGAACACTCTACCAATTTCGTCAACAAGATTTGAAATTGATGAATCAGGATTGGGAGGAATAATCGTTGATTCTGGTACAATTATTTCTCGACTACCGAGTGATGTGTATGAATCATTAAGAGAAGCATTTGTGAAGTTGACGAGTAGCCTCTCACCGGCACCAGGTATATCAGTGTTCGATACATGTTATAATCTTTCAGGTCAATCGAATGTGGAGGTCCCAACAATAGCATTTGTGTTGTCAGGAGGAACCTCGCTACGACTACCTGCAAGAAATTACTTAATTCGAGTGGACACAGCAGGAACTTATTGTTTGGCATTTATTAAAACGAAATCTTCACTTTCTATAATTGGGAGCTTCCAACAACAAGGAATACGTGTCAGCTATGACTTGACAAACTCTCTCGTCGGATTCTCAACTAATAAATGTTAGTACCATAAACTTAAAACTAATGTACAACGTCGGATTAGAATTTGGCTTAATTACAGTATTATAAGCCAATAAAATATTGTTGTTTATCATACACATGGACTTTGCCTATGAAAGTCTTAATTTAATATTGTGTTGAACTAAGCAAAAATATGTCAAAGTATTTTCTTCCTATTGCTATATGTTTTAAAGCATGTTGACTCATGAGTCAATCCTAAGAAGAAACAAATGGTACAAAAGTTTTATATTCATACAAAATATAGAAGCGTTAATGTTGAAACGG

mRNA sequence

ATCCAATAATTCATTATCTTTTTCATTCCAACATGAAGACTTCACTTTCCTCTGTTTTTCTCTTCCTAACAATCTTCACTTCCCTTCAATTTTCTTCCATTCTCTCTCGCAAGTTAACACAATCACCTTATTCCACTTCCATCTTCGATGTCTTGGCCTCCACAAACCAAGCCCTAAATGCCCTCTCCATTAAACCTAAACATCTTCAAACTCACTCTCACCTTCCAAATTCCTCTTTATCTCTGCCATTGTACCCTAGATTGTCCCTTCATAACCCTTCTTACAAGGACTACGATAGTCTTGTTAGGGCTCGACTCGCTCGTGATGCCGCTCGAGTTCAATTCCTTAACCGAAATCTTGAGCACTCTTTAAATGGGGGTAAGGATTTTGGTGAAGTTACTAATGGGTCTCTAATTGGAGATTCAATTACTGCTCCAGTTGTTTCAGGGCAAAGTAAAGGGAGTGGTGCGGAGTATTTAGCTCAGGTTGGCGTTGGTCAGCCTGTGAAGTTGTTTTATTTGGTGCCTGATACTGGCAGTGATGTCACGTGGCTTCAATGTCAGCCTTGTGCTACTGAGAACGCTTGTTATAAACAAATTGACCCGATTTTCGACCCGAAATCATCGTCTTCTTACACTCCTTTGTCTTGCAATTCTCAACAATGTGGGTTGCTAGATAGACCTAATTGCAATTCCGGCACATGTATATACCAAGTCCACTATGGTGATGGATCATTCACAACTGGTGAACTTGCCACCGAAATACTATCGTTCGGAAATTCAAATTCTATCCCTAATCTCCCAATTGGTTGTGGCCATGACAATGAAGGCTTGTTCGCTGGAGGTGCTGGTTTAATAGGTCTCGGTGGTGGTGCCATTTCCCTTTCTTCCCAATTAAAAGCGTCATCATTTTCATATTGCCTTGTCAACATGGATTCAGACTCATCCTCCACTCTTGAGTTCAACTCAAACATGCCCAGTGACTCACTAACCTCTCCACTTGTGAAAAACGATCGATTTCACTCATATAGGTACGTCAAAGTCGTTGGAATAAGTGTTGGGGGGAACACTCTACCAATTTCGTCAACAAGATTTGAAATTGATGAATCAGGATTGGGAGGAATAATCGTTGATTCTGGTACAATTATTTCTCGACTACCGAGTGATGTGTATGAATCATTAAGAGAAGCATTTGTGAAGTTGACGAGTAGCCTCTCACCGGCACCAGGTATATCAGTGTTCGATACATGTTATAATCTTTCAGGTCAATCGAATGTGGAGGTCCCAACAATAGCATTTGTGTTGTCAGGAGGAACCTCGCTACGACTACCTGCAAGAAATTACTTAATTCGAGTGGACACAGCAGGAACTTATTGTTTGGCATTTATTAAAACGAAATCTTCACTTTCTATAATTGGGAGCTTCCAACAACAAGGAATACGTGTCAGCTATGACTTGACAAACTCTCTCGTCGGATTCTCAACTAATAAATGTTAGTACCATAAACTTAAAACTAATGTACAACGTCGGATTAGAATTTGGCTTAATTACAGTATTATAAGCCAATAAAATATTGTTGTTTATCATACACATGGACTTTGCCTATGAAAGTCTTAATTTAATATTGTGTTGAACTAAGCAAAAATATGTCAAAGTATTTTCTTCCTATTGCTATATGTTTTAAAGCATGTTGACTCATGAGTCAATCCTAAGAAGAAACAAATGGTACAAAAGTTTTATATTCATACAAAATATAGAAGCGTTAATGTTGAAACGG

Coding sequence (CDS)

ATGAAGACTTCACTTTCCTCTGTTTTTCTCTTCCTAACAATCTTCACTTCCCTTCAATTTTCTTCCATTCTCTCTCGCAAGTTAACACAATCACCTTATTCCACTTCCATCTTCGATGTCTTGGCCTCCACAAACCAAGCCCTAAATGCCCTCTCCATTAAACCTAAACATCTTCAAACTCACTCTCACCTTCCAAATTCCTCTTTATCTCTGCCATTGTACCCTAGATTGTCCCTTCATAACCCTTCTTACAAGGACTACGATAGTCTTGTTAGGGCTCGACTCGCTCGTGATGCCGCTCGAGTTCAATTCCTTAACCGAAATCTTGAGCACTCTTTAAATGGGGGTAAGGATTTTGGTGAAGTTACTAATGGGTCTCTAATTGGAGATTCAATTACTGCTCCAGTTGTTTCAGGGCAAAGTAAAGGGAGTGGTGCGGAGTATTTAGCTCAGGTTGGCGTTGGTCAGCCTGTGAAGTTGTTTTATTTGGTGCCTGATACTGGCAGTGATGTCACGTGGCTTCAATGTCAGCCTTGTGCTACTGAGAACGCTTGTTATAAACAAATTGACCCGATTTTCGACCCGAAATCATCGTCTTCTTACACTCCTTTGTCTTGCAATTCTCAACAATGTGGGTTGCTAGATAGACCTAATTGCAATTCCGGCACATGTATATACCAAGTCCACTATGGTGATGGATCATTCACAACTGGTGAACTTGCCACCGAAATACTATCGTTCGGAAATTCAAATTCTATCCCTAATCTCCCAATTGGTTGTGGCCATGACAATGAAGGCTTGTTCGCTGGAGGTGCTGGTTTAATAGGTCTCGGTGGTGGTGCCATTTCCCTTTCTTCCCAATTAAAAGCGTCATCATTTTCATATTGCCTTGTCAACATGGATTCAGACTCATCCTCCACTCTTGAGTTCAACTCAAACATGCCCAGTGACTCACTAACCTCTCCACTTGTGAAAAACGATCGATTTCACTCATATAGGTACGTCAAAGTCGTTGGAATAAGTGTTGGGGGGAACACTCTACCAATTTCGTCAACAAGATTTGAAATTGATGAATCAGGATTGGGAGGAATAATCGTTGATTCTGGTACAATTATTTCTCGACTACCGAGTGATGTGTATGAATCATTAAGAGAAGCATTTGTGAAGTTGACGAGTAGCCTCTCACCGGCACCAGGTATATCAGTGTTCGATACATGTTATAATCTTTCAGGTCAATCGAATGTGGAGGTCCCAACAATAGCATTTGTGTTGTCAGGAGGAACCTCGCTACGACTACCTGCAAGAAATTACTTAATTCGAGTGGACACAGCAGGAACTTATTGTTTGGCATTTATTAAAACGAAATCTTCACTTTCTATAATTGGGAGCTTCCAACAACAAGGAATACGTGTCAGCTATGACTTGACAAACTCTCTCGTCGGATTCTCAACTAATAAATGTTAG

Protein sequence

MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQTHSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFGEVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCATENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGELATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNMDSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTIAFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLVGFSTNKC
Homology
BLAST of Pay0018781 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 2.3e-119
Identity = 235/498 (47.19%), Postives = 326/498 (65.46%), Query Frame = 0

Query: 7   SVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQTHSHLP- 66
           S+   +T+   L  +   SR L+ +P  T++ DV++S  Q    LS+ P      +  P 
Sbjct: 8   SLLAVVTLSLFLTTTDASSRSLS-TPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPE 67

Query: 67  ----------NSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNG 126
                     +S LSL L+ R +     +KDY SL  +RL RD++RV  +   +  ++ G
Sbjct: 68  SLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEG 127

Query: 127 --GKDFGEVTNGS--LIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDV 186
               D   V N       + +T PVVSG S+GSG EY +++GVG P K  YLV DTGSDV
Sbjct: 128 VDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSG-EYFSRIGVGTPAKEMYLVLDTGSDV 187

Query: 187 TWLQCQPCATENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYG 246
            W+QC+PCA    CY+Q DP+F+P SSS+Y  L+C++ QC LL+   C S  C+YQV YG
Sbjct: 188 NWIQCEPCAD---CYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYG 247

Query: 247 DGSFTTGELATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKAS 306
           DGSFT GELAT+ ++FGNS  I N+ +GCGHDNEGLF G AGL+GLGGG +S+++Q+KA+
Sbjct: 248 DGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKAT 307

Query: 307 SFSYCLVNMDSDSSSTLEFNS-NMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPIS 366
           SFSYCLV+ DS  SS+L+FNS  +     T+PL++N +  ++ YV + G SVGG  + + 
Sbjct: 308 SFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLP 367

Query: 367 STRFEIDESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSP-APGISVFDTCYNL 426
              F++D SG GG+I+D GT ++RL +  Y SLR+AF+KLT +L   +  IS+FDTCY+ 
Sbjct: 368 DAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDF 427

Query: 427 SGQSNVEVPTIAFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGI 486
           S  S V+VPT+AF  +GG SL LPA+NYLI VD +GT+C AF  T SSLSIIG+ QQQG 
Sbjct: 428 SSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGT 487

Query: 487 RVSYDLTNSLVGFSTNKC 488
           R++YDL+ +++G S NKC
Sbjct: 488 RITYDLSKNVIGLSGNKC 500

BLAST of Pay0018781 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 6.3e-93
Identity = 198/484 (40.91%), Postives = 283/484 (58.47%), Query Frame = 0

Query: 9   FLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQTH-SHLPNS 68
           F FL +   L  SS +S    Q      I DVL      L   +  P    TH S   +S
Sbjct: 7   FFFLHLHLHLSSSSSISFPDFQ------IIDVL---QPPLTVTATLPDFNNTHFSDESSS 66

Query: 69  SLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFGEVTNGSL 128
             +L L  R    + +Y+++   + AR+ RD  RV  + R +   +    D     N   
Sbjct: 67  KYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVN--- 126

Query: 129 IGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCATENACYK 188
                 + +VSG  +GSG EY  ++GVG P +  Y+V D+GSD+ W+QCQPC     CYK
Sbjct: 127 ---DFGSDIVSGMDQGSG-EYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC---KLCYK 186

Query: 189 QIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGELATEILSF 248
           Q DP+FDP  S SYT +SC S  C  ++   C+SG C Y+V YGDGS+T G LA E L+F
Sbjct: 187 QSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTF 246

Query: 249 GNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLK---ASSFSYCLVNMDSDS 308
             +  + N+ +GCGH N G+F G AGL+G+GGG++S   QL      +F YCLV+  +DS
Sbjct: 247 AKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS 306

Query: 309 SSTLEFNSN-MPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESGLGG 368
           + +L F    +P  +   PLV+N R  S+ YV + G+ VGG  +P+    F++ E+G GG
Sbjct: 307 TGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGG 366

Query: 369 IIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTIAFV 428
           +++D+GT ++RLP+  Y + R+ F   T++L  A G+S+FDTCY+LSG  +V VPT++F 
Sbjct: 367 VVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFY 426

Query: 429 LSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLVGFS 488
            + G  L LPARN+L+ VD +GTYC AF  + + LSIIG+ QQ+GI+VS+D  N  VGF 
Sbjct: 427 FTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFG 470

BLAST of Pay0018781 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 8.0e-88
Identity = 185/421 (43.94%), Postives = 251/421 (59.62%), Query Frame = 0

Query: 83  SYKDYDSLVRARLARDAARV--------QFLNRNLEHSLNGGKDFGEVTNGSLIGDSITA 142
           S K  D L  +RL RD+ RV        Q   RN+ H+   G                ++
Sbjct: 84  SNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPG--------------GFSS 143

Query: 143 PVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCATENACYKQIDPIFD 202
            VVSG S+GSG EY  ++GVG P +  Y+V DTGSD+ WLQC PC     CY Q DPIFD
Sbjct: 144 SVVSGLSQGSG-EYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC---RRCYSQSDPIFD 203

Query: 203 PKSSSSYTPLSCNSQQCGLLDRPNCNS--GTCIYQVHYGDGSFTTGELATEILSFGNSNS 262
           P+ S +Y  + C+S  C  LD   CN+   TC+YQV YGDGSFT G+ +TE L+F   N 
Sbjct: 204 PRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTF-RRNR 263

Query: 263 IPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLK---ASSFSYCLVNMDSDS--SST 322
           +  + +GCGHDNEGLF G AGL+GLG G +S   Q        FSYCLV+  + S  SS 
Sbjct: 264 VKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSV 323

Query: 323 LEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLP-ISSTRFEIDESGLGGIIV 382
           +  N+ +   +  +PL+ N +  ++ YV ++GISVGG  +P ++++ F++D+ G GG+I+
Sbjct: 324 VFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVII 383

Query: 383 DSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTIAFVLSG 442
           DSGT ++RL    Y ++R+AF     +L  AP  S+FDTC++LS  + V+VPT+      
Sbjct: 384 DSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFR- 443

Query: 443 GTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLVGFSTNK 488
           G  + LPA NYLI VDT G +C AF  T   LSIIG+ QQQG RV YDL +S VGF+   
Sbjct: 444 GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGG 484

BLAST of Pay0018781 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.3e-65
Identity = 148/397 (37.28%), Postives = 219/397 (55.16%), Query Frame = 0

Query: 97  RDAARVQFLNRNLEHSLNGGKDFGEVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQ 156
           ++  + Q L R +E      +    + NG      +   V +G       EYL  + +G 
Sbjct: 52  KNLTKFQLLERAIERGSRRLQRLEAMLNGP---SGVETSVYAGD-----GEYLMNLSIGT 111

Query: 157 PVKLFYLVPDTGSDVTWLQCQPCATENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDR 216
           P + F  + DTGSD+ W QCQPC     C+ Q  PIF+P+ SSS++ L C+SQ C  L  
Sbjct: 112 PAQPFSAIMDTGSDLIWTQCQPC---TQCFNQSTPIFNPQGSSSFSTLPCSSQLCQALSS 171

Query: 217 PNCNSGTCIYQVHYGDGSFTTGELATEILSFGNSNSIPNLPIGCGHDNEGLFAG-GAGLI 276
           P C++  C Y   YGDGS T G + TE L+FG S SIPN+  GCG +N+G   G GAGL+
Sbjct: 172 PTCSNNFCQYTYGYGDGSETQGSMGTETLTFG-SVSIPNITFGCGENNQGFGQGNGAGLV 231

Query: 277 GLGGGAISLSSQLKASSFSYCLVNMDSDSSSTLEFNSNMPSDSLTSP---LVKNDRFHSY 336
           G+G G +SL SQL  + FSYC+  + S + S L   S   S +  SP   L+++ +  ++
Sbjct: 232 GMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTTLIQSSQIPTF 291

Query: 337 RYVKVVGISVGGNTLPISSTRFEID-ESGLGGIIVDSGTIISRLPSDVYESLREAFVKLT 396
            Y+ + G+SVG   LPI  + F ++  +G GGII+DSGT ++   ++ Y+S+R+ F+   
Sbjct: 292 YYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQI 351

Query: 397 SSLSPAPGISVFDTCYNL-SGQSNVEVPTIAFVLSGGTSLRLPARNYLIRVDTAGTYCLA 456
           +        S FD C+   S  SN+++PT      GG  L LP+ NY I   + G  CLA
Sbjct: 352 NLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGG-DLELPSENYFIS-PSNGLICLA 411

Query: 457 FIKTKSSLSIIGSFQQQGIRVSYDLTNSLVGFSTNKC 488
              +   +SI G+ QQQ + V YD  NS+V F++ +C
Sbjct: 412 MGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of Pay0018781 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 4.7e-64
Identity = 146/362 (40.33%), Postives = 201/362 (55.52%), Query Frame = 0

Query: 132 ITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCATENACYKQIDP 191
           I  PV +G       EYL  V +G P   F  + DTGSD+ W QC+PC     C+ Q  P
Sbjct: 85  IETPVYAGD-----GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPC---TQCFSQPTP 144

Query: 192 IFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGELATEILSFGNSN 251
           IF+P+ SSS++ L C SQ C  L    CN+  C Y   YGDGS T G +ATE  +F  ++
Sbjct: 145 IFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYGDGSTTQGYMATETFTF-ETS 204

Query: 252 SIPNLPIGCGHDNEGLFAG-GAGLIGLGGGAISLSSQLKASSFSYCLVNMDSDSSSTLEF 311
           S+PN+  GCG DN+G   G GAGLIG+G G +SL SQL    FSYC+ +  S S STL  
Sbjct: 205 SVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLAL 264

Query: 312 N---SNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESGLGGIIVD 371
               S +P  S ++ L+ +    +Y Y+ + GI+VGG+ L I S+ F++ + G GG+I+D
Sbjct: 265 GSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIID 324

Query: 372 SGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNL-SGQSNVEVPTIAFVLSG 431
           SGT ++ LP D Y ++ +AF    +  +     S   TC+   S  S V+VP I+    G
Sbjct: 325 SGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDG 384

Query: 432 GTSLRLPARNYLIRVDTAGTYCLAF-IKTKSSLSIIGSFQQQGIRVSYDLTNSLVGFSTN 488
           G  L L  +N LI     G  CLA    ++  +SI G+ QQQ  +V YDL N  V F   
Sbjct: 385 GV-LNLGEQNILIS-PAEGVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPT 435

BLAST of Pay0018781 vs. ExPASy TrEMBL
Match: A0A5D3DYG2 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00370 PE=4 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 2.9e-274
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60
           MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60

Query: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120
           HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG
Sbjct: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120

Query: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240
           TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL
Sbjct: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240

Query: 241 ATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300
           ATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM
Sbjct: 241 ATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTI
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTI 420

Query: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
           AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 487

BLAST of Pay0018781 vs. ExPASy TrEMBL
Match: A0A5A7UQC2 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003690 PE=4 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 3.5e-272
Identity = 483/487 (99.18%), Postives = 484/487 (99.38%), Query Frame = 0

Query: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60
           MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60

Query: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120
           HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG
Sbjct: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120

Query: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240
           TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL
Sbjct: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240

Query: 241 ATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300
           ATE LSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM
Sbjct: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKV+GISVGG TLPISSTRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVIGISVGGKTLPISSTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLS QSNVEVPTI
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSSQSNVEVPTI 420

Query: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
           AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 487

BLAST of Pay0018781 vs. ExPASy TrEMBL
Match: A0A1S4E062 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo OX=3656 GN=LOC103494117 PE=4 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 1.4e-252
Identity = 457/487 (93.84%), Postives = 458/487 (94.05%), Query Frame = 0

Query: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60
           MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60

Query: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120
           HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG
Sbjct: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120

Query: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240
           TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL
Sbjct: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240

Query: 241 ATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300
           ATE LSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM
Sbjct: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKV+GISVGG TLPISSTRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVIGISVGGKTLPISSTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAP                      
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAP---------------------- 420

Query: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
                GGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 -----GGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 460

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 460

BLAST of Pay0018781 vs. ExPASy TrEMBL
Match: A0A0A0LPJ3 (Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_1G022490 PE=4 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 2.6e-251
Identity = 450/487 (92.40%), Postives = 460/487 (94.46%), Query Frame = 0

Query: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60
           M TSLSSVFLFLTIFTSLQF SILSRKLT S YSTSIFDV ASTNQAL+ALSIKPK LQ 
Sbjct: 1   MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQN 60

Query: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120
           HSHLPNS  SLPLYPRL+LHNPSYKDY++LVRARL RDAARVQFLNRNLE SLNGG  FG
Sbjct: 61  HSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG 120

Query: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           E  N SLIGDSITAPVVSGQSKGSGAEYLAQ+GVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 ESINESLIGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240
           +EN CYKQ DPIFDPKSSSSY+PLSCNSQQC LLD+ NCNS TCIYQVHYGDGSFTTGEL
Sbjct: 181 SENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGEL 240

Query: 241 ATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300
           ATE LSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVN+
Sbjct: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNL 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGG TLPIS TRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYN SGQSNVEVPTI
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTI 420

Query: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
           AFVLS GTSLRLPARNYLI +DTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 AFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 487

BLAST of Pay0018781 vs. ExPASy TrEMBL
Match: A0A5A7UQC3 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003680 PE=3 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 2.8e-200
Identity = 365/488 (74.80%), Postives = 416/488 (85.25%), Query Frame = 0

Query: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLT-QSPYSTSIFDVLASTNQALNALSIKPKHLQ 60
           M TSLS   LFLTIFT LQF SILSRKLT QSPYST+ FDV AS NQALNALSIKPK  Q
Sbjct: 2   MNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPFQ 61

Query: 61  THSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDF 120
           THS+  NS LSL L+PRL++HNPSYKDY +LVRARLAR A RVQ LNR LE SLNG K F
Sbjct: 62  THSYHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGAKQF 121

Query: 121 GEVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPC 180
           G+  NGS   +S+TAPV SG S G G EY A++GVGQPV+ F+LVPDTGSDVTWLQC+PC
Sbjct: 122 GKRINGSASTNSLTAPVTSGASHGDG-EYFARIGVGQPVQSFFLVPDTGSDVTWLQCKPC 181

Query: 181 ATENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGE 240
           A ENAC+KQ+DPIFDPKSSSSY+ LSCNS+QC LLD   C+S +CIY+V YGDGSFT GE
Sbjct: 182 ANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTIGE 241

Query: 241 LATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVN 300
           LATE LSFGNSNSIPNLPIGCGHDNEGLF   AGLIGLGGGAISLSSQL+ASSFSYCLV+
Sbjct: 242 LATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCLVD 301

Query: 301 MDSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDES 360
           +DSDSSSTL+FN++ PSDSLTSPLVKN+RF S+RYVKV+G+SVGG  LPISS+RFEIDES
Sbjct: 302 LDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEIDES 361

Query: 361 GLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPT 420
           G GGIIVDSGT I++LPSDVY+ LR+AFV LT++L  APG+S FDTCY+LS QS+VEVP 
Sbjct: 362 GSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEVPI 421

Query: 421 IAFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSL 480
           IAF+L GG SL+LPA+N LI+VD+AGT+CLAF+     LSIIG+ QQQGIRVSYDL NS+
Sbjct: 422 IAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDNSI 481

Query: 481 VGFSTNKC 488
           VGF+TNKC
Sbjct: 482 VGFATNKC 488

BLAST of Pay0018781 vs. NCBI nr
Match: TYK28409.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 953.7 bits (2464), Expect = 6.0e-274
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60
           MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60

Query: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120
           HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG
Sbjct: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120

Query: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240
           TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL
Sbjct: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240

Query: 241 ATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300
           ATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM
Sbjct: 241 ATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTI
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTI 420

Query: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
           AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 487

BLAST of Pay0018781 vs. NCBI nr
Match: KAA0058063.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 946.8 bits (2446), Expect = 7.3e-272
Identity = 483/487 (99.18%), Postives = 484/487 (99.38%), Query Frame = 0

Query: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60
           MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60

Query: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120
           HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG
Sbjct: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120

Query: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240
           TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL
Sbjct: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240

Query: 241 ATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300
           ATE LSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM
Sbjct: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKV+GISVGG TLPISSTRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVIGISVGGKTLPISSTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLS QSNVEVPTI
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSSQSNVEVPTI 420

Query: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
           AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 487

BLAST of Pay0018781 vs. NCBI nr
Match: XP_016901370.1 (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucumis melo])

HSP 1 Score: 881.7 bits (2277), Expect = 2.9e-252
Identity = 457/487 (93.84%), Postives = 458/487 (94.05%), Query Frame = 0

Query: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60
           MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60

Query: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120
           HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG
Sbjct: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120

Query: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240
           TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL
Sbjct: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240

Query: 241 ATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300
           ATE LSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM
Sbjct: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKV+GISVGG TLPISSTRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVIGISVGGKTLPISSTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAP                      
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAP---------------------- 420

Query: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
                GGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 -----GGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 460

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 460

BLAST of Pay0018781 vs. NCBI nr
Match: XP_004138238.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis sativus] >KGN63810.1 hypothetical protein Csa_014284 [Cucumis sativus])

HSP 1 Score: 877.5 bits (2266), Expect = 5.4e-251
Identity = 450/487 (92.40%), Postives = 460/487 (94.46%), Query Frame = 0

Query: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60
           M TSLSSVFLFLTIFTSLQF SILSRKLT S YSTSIFDV ASTNQAL+ALSIKPK LQ 
Sbjct: 1   MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQN 60

Query: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120
           HSHLPNS  SLPLYPRL+LHNPSYKDY++LVRARL RDAARVQFLNRNLE SLNGG  FG
Sbjct: 61  HSHLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSLNGGTHFG 120

Query: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
           E  N SLIGDSITAPVVSGQSKGSGAEYLAQ+GVGQPVKLFYLVPDTGSDVTWLQCQPCA
Sbjct: 121 ESINESLIGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180

Query: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240
           +EN CYKQ DPIFDPKSSSSY+PLSCNSQQC LLD+ NCNS TCIYQVHYGDGSFTTGEL
Sbjct: 181 SENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGSFTTGEL 240

Query: 241 ATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300
           ATE LSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVN+
Sbjct: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNL 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESG 360
           DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGG TLPIS TRFEIDESG
Sbjct: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTI 420
           LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYN SGQSNVEVPTI
Sbjct: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSNVEVPTI 420

Query: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
           AFVLS GTSLRLPARNYLI +DTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV
Sbjct: 421 AFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 487

BLAST of Pay0018781 vs. NCBI nr
Match: XP_038878113.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida])

HSP 1 Score: 788.9 bits (2036), Expect = 2.5e-224
Identity = 405/487 (83.16%), Postives = 433/487 (88.91%), Query Frame = 0

Query: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQT 60
           M  S+SS F+FLTI TSLQF SI SRKLTQSPYST+IFDV AST QA NALSIKPK  +T
Sbjct: 1   MNISISSAFIFLTILTSLQFPSIFSRKLTQSPYSTAIFDVSASTKQAQNALSIKPKPFET 60

Query: 61  HSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFG 120
           HSH PNS LSLPL+PRL+L+NPSYKDY SLVRARLARDA RVQ LNRNLE SLNGG+  G
Sbjct: 61  HSHHPNSPLSLPLHPRLTLYNPSYKDYGSLVRARLARDAVRVQSLNRNLELSLNGGQKIG 120

Query: 121 EVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCA 180
              NGS   DSITAPVVSGQS G+G EY A++GVGQPV+ FYLVPDTGSDVTWLQCQPCA
Sbjct: 121 AGINGSESADSITAPVVSGQSLGNGGEYFARIGVGQPVQSFYLVPDTGSDVTWLQCQPCA 180

Query: 181 TENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGEL 240
            E ACYKQIDPIFDPKSSSSYT LSCNSQQC LLD+ NCNS  C YQV+YGDGSFTTGEL
Sbjct: 181 AEKACYKQIDPIFDPKSSSSYTALSCNSQQCQLLDKANCNSDVCQYQVNYGDGSFTTGEL 240

Query: 241 ATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNM 300
           ATE LSFGNSNSIPNLPIGCGHDNEGLF G AGLIGLGGGAISLSSQLKASSFSYCLV++
Sbjct: 241 ATETLSFGNSNSIPNLPIGCGHDNEGLFVGAAGLIGLGGGAISLSSQLKASSFSYCLVDL 300

Query: 301 DSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESG 360
           DSDSSSTLEFN++ PSDSLTSPLVKNDRFHSYRYVKVVG+SVGGN LPISSTRFEIDESG
Sbjct: 301 DSDSSSTLEFNTDRPSDSLTSPLVKNDRFHSYRYVKVVGMSVGGNPLPISSTRFEIDESG 360

Query: 361 LGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTI 420
           LGGIIVDSGT I++LPSDVYESLREAFVK TS+L PA GIS+FDTCY+LSGQS+VEVP I
Sbjct: 361 LGGIIVDSGTTITQLPSDVYESLREAFVKQTSNLPPAQGISLFDTCYDLSGQSSVEVPVI 420

Query: 421 AFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLV 480
           AFVL G  SLRLPA+NYLI VD+ GTYCLAF KT+SSLSIIGSFQQQGIRVSYDL NSLV
Sbjct: 421 AFVLPGENSLRLPAKNYLIPVDSDGTYCLAFFKTRSSLSIIGSFQQQGIRVSYDLANSLV 480

Query: 481 GFSTNKC 488
           GFSTNKC
Sbjct: 481 GFSTNKC 487

BLAST of Pay0018781 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 455.3 bits (1170), Expect = 6.2e-128
Identity = 241/485 (49.69%), Postives = 332/485 (68.45%), Query Frame = 0

Query: 7   SVFLFLTIFTSLQFSSILSRKLTQ-SPYSTSIFDVLASTNQALNALSIKPKHLQTHSHLP 66
           S F F+   TS   SS+ SR L + S  +TSI +V  S ++     S +    +  +H  
Sbjct: 6   SFFFFIFFLTS--HSSVFSRILPETSTTTTSILNVADSIHRTKYTSSFRLNQQEEQTHSA 65

Query: 67  NSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLN--GGKDFGEV- 126
           +SS SL L+ R+S+    + DY SL  ARL RD ARV+ L   L+ ++N     D   + 
Sbjct: 66  SSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKADLKPIS 125

Query: 127 TNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCATE 186
           T  +     I AP++SG ++GSG EY  +VG+G+P +  Y+V DTGSDV WLQC PCA  
Sbjct: 126 TMYTTEEQDIEAPLISGTTQGSG-EYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCAD- 185

Query: 187 NACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGELAT 246
             CY Q +PIF+P SSSSY PLSC++ QC  L+   C + TC+Y+V YGDGS+T G+ AT
Sbjct: 186 --CYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGDFAT 245

Query: 247 EILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVNMDS 306
           E L+ G S  + N+ +GCGH NEGLF G AGL+GLGGG ++L SQL  +SFSYCLV+ DS
Sbjct: 246 ETLTIG-STLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDRDS 305

Query: 307 DSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESGLG 366
           DS+ST++F +++  D++ +PL++N +  ++ Y+ + GISVGG  L I  + FE+DESG G
Sbjct: 306 DSASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSG 365

Query: 367 GIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTIAF 426
           GII+DSGT ++RL +++Y SLR++FVK T  L  A G+++FDTCYNLS ++ VEVPT+AF
Sbjct: 366 GIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVPTVAF 425

Query: 427 VLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLVGF 486
              GG  L LPA+NY+I VD+ GT+CLAF  T SSL+IIG+ QQQG RV++DL NSL+GF
Sbjct: 426 HFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGF 483

Query: 487 STNKC 488
           S+NKC
Sbjct: 486 SSNKC 483

BLAST of Pay0018781 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 430.6 bits (1106), Expect = 1.6e-120
Identity = 235/498 (47.19%), Postives = 326/498 (65.46%), Query Frame = 0

Query: 7   SVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQTHSHLP- 66
           S+   +T+   L  +   SR L+ +P  T++ DV++S  Q    LS+ P      +  P 
Sbjct: 8   SLLAVVTLSLFLTTTDASSRSLS-TPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPE 67

Query: 67  ----------NSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNG 126
                     +S LSL L+ R +     +KDY SL  +RL RD++RV  +   +  ++ G
Sbjct: 68  SLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEG 127

Query: 127 --GKDFGEVTNGS--LIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDV 186
               D   V N       + +T PVVSG S+GSG EY +++GVG P K  YLV DTGSDV
Sbjct: 128 VDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSG-EYFSRIGVGTPAKEMYLVLDTGSDV 187

Query: 187 TWLQCQPCATENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYG 246
            W+QC+PCA    CY+Q DP+F+P SSS+Y  L+C++ QC LL+   C S  C+YQV YG
Sbjct: 188 NWIQCEPCAD---CYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYG 247

Query: 247 DGSFTTGELATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKAS 306
           DGSFT GELAT+ ++FGNS  I N+ +GCGHDNEGLF G AGL+GLGGG +S+++Q+KA+
Sbjct: 248 DGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKAT 307

Query: 307 SFSYCLVNMDSDSSSTLEFNS-NMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPIS 366
           SFSYCLV+ DS  SS+L+FNS  +     T+PL++N +  ++ YV + G SVGG  + + 
Sbjct: 308 SFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLP 367

Query: 367 STRFEIDESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSP-APGISVFDTCYNL 426
              F++D SG GG+I+D GT ++RL +  Y SLR+AF+KLT +L   +  IS+FDTCY+ 
Sbjct: 368 DAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDF 427

Query: 427 SGQSNVEVPTIAFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGI 486
           S  S V+VPT+AF  +GG SL LPA+NYLI VD +GT+C AF  T SSLSIIG+ QQQG 
Sbjct: 428 SSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGT 487

Query: 487 RVSYDLTNSLVGFSTNKC 488
           R++YDL+ +++G S NKC
Sbjct: 488 RITYDLSKNVIGLSGNKC 500

BLAST of Pay0018781 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 342.8 bits (878), Expect = 4.5e-94
Identity = 198/484 (40.91%), Postives = 283/484 (58.47%), Query Frame = 0

Query: 9   FLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQTH-SHLPNS 68
           F FL +   L  SS +S    Q      I DVL      L   +  P    TH S   +S
Sbjct: 7   FFFLHLHLHLSSSSSISFPDFQ------IIDVL---QPPLTVTATLPDFNNTHFSDESSS 66

Query: 69  SLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDFGEVTNGSL 128
             +L L  R    + +Y+++   + AR+ RD  RV  + R +   +    D     N   
Sbjct: 67  KYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVN--- 126

Query: 129 IGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCATENACYK 188
                 + +VSG  +GSG EY  ++GVG P +  Y+V D+GSD+ W+QCQPC     CYK
Sbjct: 127 ---DFGSDIVSGMDQGSG-EYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC---KLCYK 186

Query: 189 QIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGELATEILSF 248
           Q DP+FDP  S SYT +SC S  C  ++   C+SG C Y+V YGDGS+T G LA E L+F
Sbjct: 187 QSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTF 246

Query: 249 GNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLK---ASSFSYCLVNMDSDS 308
             +  + N+ +GCGH N G+F G AGL+G+GGG++S   QL      +F YCLV+  +DS
Sbjct: 247 AKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS 306

Query: 309 SSTLEFNSN-MPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDESGLGG 368
           + +L F    +P  +   PLV+N R  S+ YV + G+ VGG  +P+    F++ E+G GG
Sbjct: 307 TGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGG 366

Query: 369 IIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTIAFV 428
           +++D+GT ++RLP+  Y + R+ F   T++L  A G+S+FDTCY+LSG  +V VPT++F 
Sbjct: 367 VVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFY 426

Query: 429 LSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLVGFS 488
            + G  L LPARN+L+ VD +GTYC AF  + + LSIIG+ QQ+GI+VS+D  N  VGF 
Sbjct: 427 FTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFG 470

BLAST of Pay0018781 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 333.6 bits (854), Expect = 2.7e-91
Identity = 211/508 (41.54%), Postives = 298/508 (58.66%), Query Frame = 0

Query: 1   MKTSLSSVF--LFLTIFTSLQFSSILSRKLTQS-----PYSTSIFDVLASTNQALNALSI 60
           + T   SVF  LF T   S Q+ +++   L  S     P S S+ D   S +++  +LS+
Sbjct: 6   LNTLAFSVFAVLFFTSSASSQYQTLVVNTLPSSATLSWPESESLTD--ESLSESTTSLSV 65

Query: 61  KPKHLQTHSHLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSL 120
              H+   S   ++S +                   L   RL RD+ RV+ +      ++
Sbjct: 66  HLSHVDALSSFSDASPA------------------DLFNLRLQRDSLRVKSITSLA--AV 125

Query: 121 NGGKDFGEVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTW 180
           + G++  + T  +  G   +  V+SG S+GSG EY  ++GVG P    Y+V DTGSDV W
Sbjct: 126 STGRNATKRTPRTAGG--FSGAVISGLSQGSG-EYFMRLGVGTPATNVYMVLDTGSDVVW 185

Query: 181 LQCQPCATENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLL-DRPNC---NSGTCIYQVH 240
           LQC PC    ACY Q D IFDPK S ++  + C S+ C  L D   C    S TC+YQV 
Sbjct: 186 LQCSPC---KACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVS 245

Query: 241 YGDGSFTTGELATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLK 300
           YGDGSFT G+ +TE L+F  +  + ++P+GCGHDNEGLF G AGL+GLG G +S  SQ K
Sbjct: 246 YGDGSFTEGDFSTETLTFHGAR-VDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTK 305

Query: 301 ---ASSFSYCLVNMDSDSS-----STLEF-NSNMPSDSLTSPLVKNDRFHSYRYVKVVGI 360
                 FSYCLV+  S  S     ST+ F N+ +P  S+ +PL+ N +  ++ Y++++GI
Sbjct: 306 NRYNGKFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGI 365

Query: 361 SVGGNTLP-ISSTRFEIDESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPG 420
           SVGG+ +P +S ++F++D +G GG+I+DSGT ++RL    Y +LR+AF    + L  AP 
Sbjct: 366 SVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPS 425

Query: 421 ISVFDTCYNLSGQSNVEVPTIAFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLS 480
            S+FDTC++LSG + V+VPT+ F   GG  + LPA NYLI V+T G +C AF  T  SLS
Sbjct: 426 YSLFDTCFDLSGMTTVKVPTVVFHFGGG-EVSLPASNYLIPVNTEGRFCFAFAGTMGSLS 483

Query: 481 IIGSFQQQGIRVSYDLTNSLVGFSTNKC 488
           IIG+ QQQG RV+YDL  S VGF +  C
Sbjct: 486 IIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

BLAST of Pay0018781 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 325.9 bits (834), Expect = 5.7e-89
Identity = 185/421 (43.94%), Postives = 251/421 (59.62%), Query Frame = 0

Query: 83  SYKDYDSLVRARLARDAARV--------QFLNRNLEHSLNGGKDFGEVTNGSLIGDSITA 142
           S K  D L  +RL RD+ RV        Q   RN+ H+   G                ++
Sbjct: 84  SNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPG--------------GFSS 143

Query: 143 PVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPCATENACYKQIDPIFD 202
            VVSG S+GSG EY  ++GVG P +  Y+V DTGSD+ WLQC PC     CY Q DPIFD
Sbjct: 144 SVVSGLSQGSG-EYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC---RRCYSQSDPIFD 203

Query: 203 PKSSSSYTPLSCNSQQCGLLDRPNCNS--GTCIYQVHYGDGSFTTGELATEILSFGNSNS 262
           P+ S +Y  + C+S  C  LD   CN+   TC+YQV YGDGSFT G+ +TE L+F   N 
Sbjct: 204 PRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTF-RRNR 263

Query: 263 IPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLK---ASSFSYCLVNMDSDS--SST 322
           +  + +GCGHDNEGLF G AGL+GLG G +S   Q        FSYCLV+  + S  SS 
Sbjct: 264 VKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSV 323

Query: 323 LEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLP-ISSTRFEIDESGLGGIIV 382
           +  N+ +   +  +PL+ N +  ++ YV ++GISVGG  +P ++++ F++D+ G GG+I+
Sbjct: 324 VFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVII 383

Query: 383 DSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPTIAFVLSG 442
           DSGT ++RL    Y ++R+AF     +L  AP  S+FDTC++LS  + V+VPT+      
Sbjct: 384 DSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFR- 443

Query: 443 GTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSLVGFSTNK 488
           G  + LPA NYLI VDT G +C AF  T   LSIIG+ QQQG RV YDL +S VGF+   
Sbjct: 444 GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGG 484

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LS402.3e-11947.19Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LHE36.3e-9340.91Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LNJ38.0e-8843.94Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q766C33.3e-6537.28Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C24.7e-6440.33Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A5D3DYG22.9e-274100.00Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A5A7UQC23.5e-27299.18Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S4E0621.4e-25293.84protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0LPJ32.6e-25192.40Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_1G022490 PE=... [more]
A0A5A7UQC32.8e-20074.80Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo var. makuwa OX=1194695... [more]
Match NameE-valueIdentityDescription
TYK28409.16.0e-274100.00protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucumis melo var. makuwa][more]
KAA0058063.17.3e-27299.18protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucumis melo var. makuwa][more]
XP_016901370.12.9e-25293.84PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucumis melo][more]
XP_004138238.15.4e-25192.40protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis sativus] >KGN63810.1 hypothet... [more]
XP_038878113.12.5e-22483.16protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G25510.16.2e-12849.69Eukaryotic aspartyl protease family protein [more]
AT3G18490.11.6e-12047.19Eukaryotic aspartyl protease family protein [more]
AT3G20015.14.5e-9440.91Eukaryotic aspartyl protease family protein [more]
AT3G61820.12.7e-9141.54Eukaryotic aspartyl protease family protein [more]
AT1G01300.15.7e-8943.94Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 148..310
e-value: 2.5E-46
score: 158.1
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 334..483
e-value: 2.0E-36
score: 125.2
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 125..309
e-value: 5.8E-48
score: 165.4
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 310..487
e-value: 1.8E-48
score: 166.6
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 144..487
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 21..487
NoneNo IPR availablePANTHERPTHR13683:SF274PROTEIN ASPARTIC PROTEASE IN GUARD CELL 1coord: 21..487
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 148..483
score: 43.419895

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0018781.1Pay0018781.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity