Pay0003099 (gene) Melon (Payzawat) v1

BLAST of Pay0003099 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 4.5e-57
Identity = 162/530 (30.57%), Postives = 275/530 (51.89%), Query Frame = 0

Query: 15  KWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAKDLGKAFGLLAGLSS 74
           KW  LV+AIW+Q+ +G N+ FS YS+ LKS+L +SQ++LN L+VA DLGKAFG  +G++ 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 75  DRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLC--MGGNSTTWMNTGVL 134
                S++LF  +  G +GYG QWLV++  I  LPY    +FLC  + G S  W NT   
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVIT-NIITLPY--SLVFLCCLLAGLSICWFNTACF 162

Query: 135 VTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALLSLVPFIVCLTAI 194
           + C+R+F  +R     +   +  +S A+++   +A+   + + +L L SLVP +V   A+
Sbjct: 163 ILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAAL 222

Query: 195 LFLREIPC-----SADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLSRIFSIIL 254
             +   P        D    +   F +LN ++V  +  LL+  S    ++ L+ I +++L
Sbjct: 223 YPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLL-SSSSTSSARLNFIGAVVL 282

Query: 255 LSL-LASPLVI-------PLHSFLKNRGGSGEV------------GEALLAGESAADDAV 314
           L   L +PL++       P+ +   N   SG V              +   G      A 
Sbjct: 283 LVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAK 342

Query: 315 EGKPA-IGEDHTIVEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFIS 374
           EG    +G++H+    +  +EFW+ ++++ CG   GL   NN+GQI  +LG +  ++ ++
Sbjct: 343 EGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNSTTL-VT 402

Query: 375 LMSIWGFFGRILSGSASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAI--PGSLYIGS 434
           + S + FFGR+LS +A +   K+  + R  W A + +   + + +LA++     +L   +
Sbjct: 403 IYSSFSFFGRLLS-AAPDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTAT 462

Query: 435 IVVGICYGVRLAITVPMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEA-- 494
            ++G+  G   A  V + S++FG  + G+ +N+LI N+P+GS L+ G +A  +Y+  A  
Sbjct: 463 ALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLY-GYIAASIYEANASP 522

Query: 495 --TTTKTGGNTCTGAHCYRLVFVVMACSCGIGFAMDLFLAFRTKRLYSKM 511
             T   +    C G  CY   FV   C   +G    L L  RTK +Y ++
Sbjct: 523 DITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHRL 565

BLAST of Pay0003099 vs. ExPASy Swiss-Prot
Match: Q5AXV1 (Probable transporter mch1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mch1 PE=3 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 6.6e-08
Identity = 124/568 (21.83%), Postives = 214/568 (37.68%), Query Frame = 0

Query: 5   RRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAKDLGK 64
           RR      +  W G+++ +   SI+     FS Y   L + LN +QL++N +S+A  +  
Sbjct: 75  RREVVRVGSFVW-GVITCLGAGSIT----AFSLYGPLLLTRLNYTQLRVNEVSIAAGISM 134

Query: 65  AFGL-LAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKP------LPYWQMCIFL 124
              + LAG   DR S S L     I   +GY     V      P       P+W M +  
Sbjct: 135 YLPVSLAGYLCDRYSPSPLTLFAGIAFGLGYSLAAFVYKSGPPPDAGGNGWPFWVMVVAF 194

Query: 125 CMGGNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIF------------TDLC 184
              G +T  M    + TC +NF   RG   GI+   +A+  A F              LC
Sbjct: 195 IAIGVATCSMYLAAVTTCAKNF--GRGKHKGII---LAVPIAAFGLSGMWQSQVGTYFLC 254

Query: 185 SALFSDNPSS--------FLALLSLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNA 244
             L   N           FLA+L L   ++   A+  +       DDE E +    +  A
Sbjct: 255 ERLKDSNCGDVDVYKYFLFLAILLLTIGVIGTFALRIV-------DDEEEKY----IDEA 314

Query: 245 VSVAVAVTLLVFDSIPNPNSFLSRIFSIILLSLLASPLVIPLHSFLKNRGGSGEV-GEAL 304
           V       LL       P S +   +                 +F  +   +G V  +++
Sbjct: 315 VEELERSGLLAESEFFRPRSEVQAAYG----------------TFSDDHEDNGSVDDQSV 374

Query: 305 LAGESAADDAVEGKPAIGEDHTIVEAMKTVE---------FWILFVSFLCGVGTGLAVMN 364
              E   + A   K    E+      +   E          W L + F    G G A +N
Sbjct: 375 TISEELREAARREKEREEEERRKKNWLLNYETRIFLADHTMWWLALGFFLVTGPGEAYLN 434

Query: 365 NMGQIGLALGYDDVSI-----------FISLMSIWGFFGRILSGSASE--------HFI- 424
           N+G I   L  D  +I            ++++++     R+L+GS S+        HF  
Sbjct: 435 NLGTIVQTLNLDTTAIVDSHPAGLPSTHVTIIALTSTIARLLTGSLSDLFAPTARRHFTV 494

Query: 425 ------------KKAGMPRPLWNAASQILMALGYIVLAIAIPGS----LYIGSIVVGICY 484
                       ++  + R  +   S +L++LG+++LA  +P       ++ + +VG+ Y
Sbjct: 495 DQETAGPDPFTKQRPALSRLAFLIPSALLLSLGFLLLASPLPTHHPELSHLTTALVGLGY 554

Query: 485 GVRLAITVPMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNT 500
           G   ++   + S V+G++N+G  + ++ +  P       G++    Y   A  + T    
Sbjct: 555 GSIFSLVPIIISVVWGVENFGTNWGIVAM-FPAAGAAMWGVIYSRAYQSAADGSPTDDGQ 602

BLAST of Pay0003099 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 6.8e-05
Identity = 55/216 (25.46%), Postives = 91/216 (42.13%), Query Frame = 0

Query: 295 EDHTIVEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDV-----SIFISLMS 354
           E  T+ E       W+  + F+   G      NNMG I   +  ++      S  +SL +
Sbjct: 261 EGATLKEFFTDKTAWLFLLCFVFIGGPFEMFQNNMGAILDTVTVENADSPSFSTHVSLFA 320

Query: 355 IWGFFGRILSGSASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLY-------- 414
            +    R++ G +SE        P  L       ++AL    + + +P  ++        
Sbjct: 321 TFSTVSRLVVGFSSEAMESHVSRPVLL------SVIALVAACIHLMVPSGIFTVFDNAKY 380

Query: 415 --IGSIVVGICYGVRLAITVPMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYD 474
             + +IV G  YG    +   + ++V+G+ N G I+   IL L +GS L  GLL   +YD
Sbjct: 381 FSVVTIVNGFSYGSSFTLVPTIVTKVWGIANLGTIWGSFILALAVGS-LGYGLLFAKVYD 440

Query: 475 MEATT-TKTGGNTCTGAHCYRLVFVVMACSCGIGFA 495
             +     +    C+G HCY L FV+     G+ FA
Sbjct: 441 AASEVGVGSMSQVCSGVHCYGLTFVI--TGTGLAFA 467

BLAST of Pay0003099 vs. ExPASy Swiss-Prot
Match: Q4WVT3 (Probable transporter mch1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mch1 PE=3 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 2.6e-04
Identity = 115/544 (21.14%), Postives = 210/544 (38.60%), Query Frame = 0

Query: 17  LGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAKDLGKAFGL-LAGLSSD 76
           +G++S +   SI+     FS Y     + L+ SQLQ+N +S+A ++     + L G   D
Sbjct: 86  VGVISCLGAGSIT----AFSLYGPLFLTRLHYSQLQVNAVSIAAEISMYLPVPLFGYLCD 145

Query: 77  RLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPY--------WQMCIFLCMGGNSTTWM 136
           R + S L  +  +  + G G      + +  PLP         W M +     G +T+ M
Sbjct: 146 RYTPSPLALLSGL--VFGGGYLLAAFAYRSGPLPEAGGEGWPPWVMVVAFVAIGTATSCM 205

Query: 137 NTGVLVTCLRNFRR--DRGTVSGILKGYIALSTAIFTDLCSALFSDNPSS--------FL 196
               + TC +NF R   +G +  +      LS    + + + L  +            F 
Sbjct: 206 YLAAVTTCAKNFGRGKHKGIMLAVPIAGFGLSGMWQSQVATYLLCERREDGSRGDVDVFR 265

Query: 197 ALLSLVPFIVCLTAI--LFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNP 256
             L L  F+ CL  I    LR +    D+E + +    +  AV       LL       P
Sbjct: 266 YFLFLALFLFCLGVIGTFGLRIV----DEEEDQY----IDEAVEELERSGLLEESEFFRP 325

Query: 257 NSFLSRIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGE 316
            S +   +     +         L   L     S E  EA    +   ++    K  +  
Sbjct: 326 RSEVQAAYGTFSDAADGDAPGPELSLTL-----SEEEREAARLEKEREEEERRKKNWLLN 385

Query: 317 DHTIVEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYD-----------DVSIF 376
             T +  ++    W L V F    G G A +NN+G I   L  +             S  
Sbjct: 386 FETRL-FLQDQTMWWLAVGFFLVTGPGEAYINNLGTIIQTLTPELHPPNAPSPAGLPSTH 445

Query: 377 ISLMSIWGFFGRILSGSASEHF------------------------IKKAGMPRPLWNAA 436
           ++++++     R+L+GS S+ F                         K+  + R  +   
Sbjct: 446 VTIIALTSTIARLLTGSLSDFFAPPATHLFPANIESGRRSSQSGPTAKRPTLSRLAFLLP 505

Query: 437 SQILMALGYIVLAIAI----PGSLYIGSIVVGICYGVRLAITVPMASEVFGLKNYGMIYN 496
           S +L++LGY++L+  +    PG  ++ + ++G+ YG   ++   + S V+G++N+G  + 
Sbjct: 506 SALLLSLGYLLLSSPLPLQHPGLSHVTTALIGLGYGSAFSLVPIIISVVWGVENFGTNWG 565

BLAST of Pay0003099 vs. ExPASy Swiss-Prot
Match: Q03795 (Uncharacterized membrane protein YMR155W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YMR155W PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 3.4e-04
Identity = 114/509 (22.40%), Postives = 198/509 (38.90%), Query Frame = 0

Query: 29  SGNNYTFSNYSAALKSLLNLSQLQLNNLSVAKDLGKA-FGLLAGLSSDRLSTSLLLFIGS 88
           +G  Y FS Y+  L S  ++     + LS +  +G +  G+LAG+  DR S  L   IGS
Sbjct: 32  AGTPYLFSFYAPQLLSKCHIPVSASSKLSFSLTIGSSLMGILAGIVVDR-SPKLSCLIGS 91

Query: 89  IEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMGGNSTTWMNTGVLVTCLR-NFRRDRGTV 148
           +   I Y    L    +        + + L   G+ + +  +   V C   NF + RGT 
Sbjct: 92  MCVFIAYLILNLCYKHEWSSTFLISLSLVLIGYGSVSGFYAS---VKCANTNFPQHRGTA 151

Query: 149 SGILKGYIALSTAIFTDLCSALFSDNPSSFLALLSLVPFIVCLTAIL---FLREIPCSAD 208
                    LS  +F+ LCS LF +N       L     + C   IL   F  +I  +A+
Sbjct: 152 GAFPVSLYGLSGMVFSYLCSKLFGENIEHVFIFL----MVACGCMILVGYFSLDIFSNAE 211

Query: 209 DETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFL-SRIFSIILLSLLASPLVIPLHSF 268
            +  + K  W L          + +++   N N ++ S + S       +SP     ++ 
Sbjct: 212 GDDASIKE-WELQKSRETDDNIVPLYE---NSNDYIGSPVRS-------SSPATYETYAL 271

Query: 269 LKNRGGSGEV---------GEALLAGESA-----ADDAVEGKPAIGEDHT-------IVE 328
             N   + E             LL+  S       +D    K  +GE+         + +
Sbjct: 272 SDNFQETSEFFALEDRQLSNRPLLSPSSPHTKYDFEDENTSKNTVGENSAQKSMRLHVFQ 331

Query: 329 AMKTVEFWILFVSFLCGVGTGLAVMNNMG-----QIG------LALGYDDV-SIFISLMS 388
           ++K+  F   ++      G GL  + ++G     Q+       L +  + + S+ ++L+S
Sbjct: 332 SLKSSTFIGYYIVLGILQGVGLMYIYSVGFMVQAQVSTPPLNQLPINAEKIQSLQVTLLS 391

Query: 389 IWGFFGRILSGSASEHFIKKAGMPRPLWNAASQILMA------LGYIVLAIAIPG----- 448
           +  F GR+ SG  S+  +KK    R LWN     L+       + +   +I  P      
Sbjct: 392 LLSFCGRLSSGPISDFLVKKFKAQR-LWNIVIASLLVFLASNKISHDFSSIEDPSLRASK 451

Query: 449 ---SLYIGSIVVGICYGVRLAITVPMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAG 485
              ++ + S + G  +GV       + ++ FG   Y  ++ VL         +F+ +L  
Sbjct: 452 SFKNISVCSAIFGYSFGVLFGTFPSIVADRFGTNGYSTLWGVLTTGGVFSVSVFTDILG- 511

BLAST of Pay0003099 vs. ExPASy TrEMBL
Match: A0A1S3BI29 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103490296 PE=4 SV=1)

HSP 1 Score: 994.2 bits (2569), Expect = 2.0e-286
Identity = 514/515 (99.81%), Postives = 515/515 (100.00%), Query Frame = 0

Query: 1   MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAK 60
           MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAK
Sbjct: 25  MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAK 84

Query: 61  DLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMG 120
           DLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMG
Sbjct: 85  DLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMG 144

Query: 121 GNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 180
           GNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL
Sbjct: 145 GNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 204

Query: 181 SLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS 240
           SLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS
Sbjct: 205 SLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS 264

Query: 241 RIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHTIV 300
           RIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHTIV
Sbjct: 265 RIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHTIV 324

Query: 301 EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSG 360
           EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGY+DVSIFISLMSIWGFFGRILSG
Sbjct: 325 EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYEDVSIFISLMSIWGFFGRILSG 384

Query: 361 SASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVP 420
           SASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVP
Sbjct: 385 SASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVP 444

Query: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYRL 480
           MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYRL
Sbjct: 445 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYRL 504

Query: 481 VFVVMACSCGIGFAMDLFLAFRTKRLYSKMRALKR 516
           VFVVMACSCGIGFAMDLFLAFRTKRLYSKMRALKR
Sbjct: 505 VFVVMACSCGIGFAMDLFLAFRTKRLYSKMRALKR 539

BLAST of Pay0003099 vs. ExPASy TrEMBL
Match: A0A6J1JP36 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111486233 PE=4 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 1.5e-228
Identity = 411/512 (80.27%), Postives = 456/512 (89.06%), Query Frame = 0

Query: 1   MNIFR-RSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVA 60
           M+IF    PPSSSA+KWL LVSA+WVQSI+GNNYTFSNYSAALKSL+NLSQLQLNNLSVA
Sbjct: 1   MSIFHPPPPPSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVA 60

Query: 61  KDLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCM 120
           KDLGKAFGLL+GL+SDRL TSLLL IGS+EGLIGYG QWLVVS+ I PLPYWQ+ IF+CM
Sbjct: 61  KDLGKAFGLLSGLASDRLPTSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCM 120

Query: 121 GGNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLAL 180
           GGNS+TWMNT VLVTCLRNFRR+ G+VSG+LKGY+ALSTAIFTDLC ALFS++PSSFLAL
Sbjct: 121 GGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLAL 180

Query: 181 LSLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFL 240
           L++VPF VCL AILFLRE P + D+ETE+ +YFWVLNA++VAVAV+LL FD IPNP S +
Sbjct: 181 LAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDLIPNPTSSV 240

Query: 241 SRIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHTI 300
           S IFSI+LL LL SPLVIP+HSFLKNRG SG   EALLA E  A+    G P IGEDHTI
Sbjct: 241 SIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHTI 300

Query: 301 VEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILS 360
           VEAMKTVEFW+LFVSFL GVGTGLAVMNNMGQIGLALGYDDVS FISLMSIWGFFGRILS
Sbjct: 301 VEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRILS 360

Query: 361 GSASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITV 420
           GSASE+FIKKAGM RPLWNAASQILMA+GYI+LAI +P SLYIGSI+VGICYGVRLAI+V
Sbjct: 361 GSASEYFIKKAGMARPLWNAASQILMAIGYILLAIGMPSSLYIGSIIVGICYGVRLAISV 420

Query: 421 PMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYR 480
           P+ASE+FGLK+YGMIYNVLILNLPLGSFLFSGLLAGILYDMEAT  + GGNTC GAHCYR
Sbjct: 421 PIASEIFGLKDYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATKIEEGGNTCMGAHCYR 480

Query: 481 LVFVVMACSCGIGFAMDLFLAFRTKRLYSKMR 512
           LVFVVMACSC +G  +DL L  +TKRLYSK R
Sbjct: 481 LVFVVMACSCVVGLGVDLLLFIKTKRLYSKTR 512

BLAST of Pay0003099 vs. ExPASy TrEMBL
Match: A0A6J1ENR3 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111435199 PE=4 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 1.3e-227
Identity = 410/513 (79.92%), Postives = 455/513 (88.69%), Query Frame = 0

Query: 1   MNIFRRSPP--SSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSV 60
           M+IF   PP  SSSA+KWL LVSA+WVQSI+GNNYTFSNYSAALKSL+NLSQLQLNNLSV
Sbjct: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLC 120
           AKDLGKAFGLL+GL+SDRL  SLLL IGS+EGLIGYG QWLVVS+ I PLPYWQ+ IF+C
Sbjct: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120

Query: 121 MGGNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLA 180
           MGGNS+TWMNT VLVTCLRNFRR+ G+VSG+LKGY+ALSTAIFTDLC ALFS++PSSFLA
Sbjct: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180

Query: 181 LLSLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSF 240
           LL++VPF VCL AILFLRE P + D+ETE+ +YFWVLNA++VAVAV+LL FDSIPNP S 
Sbjct: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240

Query: 241 LSRIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHT 300
           +S IFSI+LL LL SPLVIP+HSFLKNRG SG   EALLA E  A+    G P IGEDHT
Sbjct: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300

Query: 301 IVEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRIL 360
           IVEAMKTVEFW+LFVSFL GVGTGLAVMNNMGQIGLALGYDDVS FISLMSIWGFFGRIL
Sbjct: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360

Query: 361 SGSASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAIT 420
           SGSASE+FIKKAGM RPLWNAASQILMA+G I+LAI +P SLYIGSI+VGICYGVRLAI+
Sbjct: 361 SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAIS 420

Query: 421 VPMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCY 480
           VP+ASE+FGLK+YGMIYNVLI NLPLGSFLFSGLLAGILYDMEAT T+ GGNTC G HCY
Sbjct: 421 VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCY 480

Query: 481 RLVFVVMACSCGIGFAMDLFLAFRTKRLYSKMR 512
           RLVFVVMACSC +G  +DL L  RTKRLYSK+R
Sbjct: 481 RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIR 513

BLAST of Pay0003099 vs. ExPASy TrEMBL
Match: A0A6A6LNL6 (Nodulin-like domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714_031577 PE=4 SV=1)

HSP 1 Score: 727.6 bits (1877), Expect = 3.6e-206
Identity = 383/550 (69.64%), Postives = 446/550 (81.09%), Query Frame = 0

Query: 1   MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAK 60
           M   + S    +A KWLG V+A+WVQ+ISGNNYTFSNYS ALK+L+NL+QLQLN+LSVAK
Sbjct: 1   MGFLQNSSTFFTAAKWLGFVTAVWVQAISGNNYTFSNYSDALKTLMNLTQLQLNSLSVAK 60

Query: 61  DLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMG 120
           D+GKAFGLLAGL+SDRL T ++L IGSIEGLIGYGAQWLVVSR+I+PL YWQMCIFLC+G
Sbjct: 61  DVGKAFGLLAGLASDRLPTPVILLIGSIEGLIGYGAQWLVVSRRIQPLAYWQMCIFLCLG 120

Query: 121 GNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 180
           GNSTTWMNT VLVTC+RNFRR+RG VSGILKGY+ LSTAIFTDLCSALFSDNP+SFL +L
Sbjct: 121 GNSTTWMNTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCSALFSDNPASFLLML 180

Query: 181 SLVPFIVCLTAILFLREIP--CSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSF 240
           +++PF VCL+AILFLREIP   +A++E E FKYF V NAV++ VAV LL +D I NP+S 
Sbjct: 181 AIIPFAVCLSAILFLREIPPAATAEEEKEEFKYFSVFNAVAIVVAVYLLAYDFISNPSSA 240

Query: 241 LSRIFSIILLSLLASPLVIPLHSF--------LKNRGGSGE--VGEALLAG-------ES 300
            S +FSIILL LLASPL IP++SF        LKN+    E  + E LL G       E 
Sbjct: 241 FSLVFSIILLVLLASPLAIPVYSFVKSWNLNRLKNKPADVEEHIDEPLLNGKIEEEIQEK 300

Query: 301 AADDA----------------VEGKPAIGEDHTIVEAMKTVEFWILFVSFLCGVGTGLAV 360
            A++A                V+ KP IGEDHTI+EAM  ++FWILF SFLCGVGTGLAV
Sbjct: 301 PAEEAEPAVVTTQPPADELAEVKRKPVIGEDHTILEAMMKLDFWILFFSFLCGVGTGLAV 360

Query: 361 MNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSGSASEHFIKKAGMPRPLWNAASQILM 420
           MNNMGQIGLALGY DVSIF+SL SIWGFFGRI SGS SE FIKKAG PRPLWNAASQILM
Sbjct: 361 MNNMGQIGLALGYADVSIFVSLTSIWGFFGRIGSGSISEFFIKKAGTPRPLWNAASQILM 420

Query: 421 ALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVPMASEVFGLKNYGMIYNVLILNLPLG 480
           A+GYI++A+A+PGSLYIGS+VVGICYGVRLA+TVP ASE+FGLK YG+IYN+LILNLPLG
Sbjct: 421 AVGYILMALAMPGSLYIGSVVVGICYGVRLAVTVPTASELFGLKYYGLIYNILILNLPLG 480

Query: 481 SFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYRLVFVVMACSCGIGFAMDLFLAFRTKR 516
           S LFSGLLAG+LYD EAT T  GGNTC GAHCYRLVF++MA +C IGF +D+ LA RTK+
Sbjct: 481 SLLFSGLLAGLLYDAEATPTPGGGNTCVGAHCYRLVFIIMAIACCIGFGLDVLLATRTKK 540

BLAST of Pay0003099 vs. ExPASy TrEMBL
Match: A0A2C9W1Q6 (Nodulin-like domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_04G073000 PE=4 SV=1)

HSP 1 Score: 727.6 bits (1877), Expect = 3.6e-206
Identity = 379/552 (68.66%), Postives = 442/552 (80.07%), Query Frame = 0

Query: 1   MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAK 60
           M   ++S    +A KWLG V+A+WVQ+ISGNNYTFSNYS ALK+L+NL+QLQLN LSVAK
Sbjct: 1   MAFLQKSSTFFAATKWLGFVTAVWVQAISGNNYTFSNYSDALKTLMNLTQLQLNGLSVAK 60

Query: 61  DLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMG 120
           D+GKAFGLLAGL+SDRL T ++L IGSIEGLIGYGAQW VVS++I+PL YWQMCIFLC+G
Sbjct: 61  DVGKAFGLLAGLASDRLPTPVILLIGSIEGLIGYGAQWFVVSKRIQPLAYWQMCIFLCLG 120

Query: 121 GNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 180
           GNSTTWMNT VLVTC+RNFRR+RG VSGILKGY+ LSTAIFTDLCSALFSDNP+SFL +L
Sbjct: 121 GNSTTWMNTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCSALFSDNPASFLLML 180

Query: 181 SLVPFIVCLTAILFLREIP--CSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSF 240
           +++PF +CLTAILFLREIP   +A++E E FKYF V N V++ VAV LLV+D I NP+  
Sbjct: 181 AIIPFFICLTAILFLREIPPAATAEEEKEEFKYFSVFNVVAIVVAVYLLVYDFISNPSRS 240

Query: 241 LSRIFSIILLSLLASPLVIPLHSFLKN--------------------------------- 300
            S +FSIILL LLASPL +P++SF+K+                                 
Sbjct: 241 FSLVFSIILLFLLASPLAVPVYSFVKSWNLKRLKNKPADVEEHIDEPLLSEEIEEDIDIQ 300

Query: 301 RGGSGEVGEALLAGESAADDAVE--GKPAIGEDHTIVEAMKTVEFWILFVSFLCGVGTGL 360
           +  S E   A++A +  AD+  E   KP IGEDHTI EAM  ++FWILFVSFLCGVGTGL
Sbjct: 301 QKPSEEAALAVVAAQPPADEVAEMRKKPVIGEDHTIFEAMTKLDFWILFVSFLCGVGTGL 360

Query: 361 AVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSGSASEHFIKKAGMPRPLWNAASQI 420
           AVMNNMGQIGLALGY DVSIF+SL SIWGFFGRI SGS SE FIKKAG PRPLWNAASQI
Sbjct: 361 AVMNNMGQIGLALGYADVSIFVSLTSIWGFFGRIGSGSISEFFIKKAGTPRPLWNAASQI 420

Query: 421 LMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVPMASEVFGLKNYGMIYNVLILNLP 480
           LMA+GYI++A A+PGSLYIGSIVVGICYGVRLA+TVP ASE+FGLK YG+IYN+LILNLP
Sbjct: 421 LMAVGYILMASAMPGSLYIGSIVVGICYGVRLAVTVPTASELFGLKYYGLIYNILILNLP 480

Query: 481 LGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYRLVFVVMACSCGIGFAMDLFLAFRT 516
           LGSFLFSGLLAG LYD EAT T  GGNTCTGAHCYRLVF++MA +C IGF +D+ LA RT
Sbjct: 481 LGSFLFSGLLAGFLYDAEATPTAGGGNTCTGAHCYRLVFIIMAIACFIGFGLDVLLAIRT 540

BLAST of Pay0003099 vs. NCBI nr
Match: XP_008447967.2 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo])

HSP 1 Score: 994.2 bits (2569), Expect = 4.2e-286
Identity = 514/515 (99.81%), Postives = 515/515 (100.00%), Query Frame = 0

Query: 1   MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAK 60
           MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAK
Sbjct: 25  MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAK 84

Query: 61  DLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMG 120
           DLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMG
Sbjct: 85  DLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMG 144

Query: 121 GNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 180
           GNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL
Sbjct: 145 GNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 204

Query: 181 SLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS 240
           SLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS
Sbjct: 205 SLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS 264

Query: 241 RIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHTIV 300
           RIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHTIV
Sbjct: 265 RIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHTIV 324

Query: 301 EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSG 360
           EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGY+DVSIFISLMSIWGFFGRILSG
Sbjct: 325 EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYEDVSIFISLMSIWGFFGRILSG 384

Query: 361 SASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVP 420
           SASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVP
Sbjct: 385 SASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVP 444

Query: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYRL 480
           MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYRL
Sbjct: 445 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYRL 504

Query: 481 VFVVMACSCGIGFAMDLFLAFRTKRLYSKMRALKR 516
           VFVVMACSCGIGFAMDLFLAFRTKRLYSKMRALKR
Sbjct: 505 VFVVMACSCGIGFAMDLFLAFRTKRLYSKMRALKR 539

BLAST of Pay0003099 vs. NCBI nr
Match: XP_011658596.1 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis sativus] >KAE8645667.1 hypothetical protein Csa_020437 [Cucumis sativus])

HSP 1 Score: 946.0 bits (2444), Expect = 1.3e-271
Identity = 486/515 (94.37%), Postives = 498/515 (96.70%), Query Frame = 0

Query: 1   MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAK 60
           MN FRRSPPS SALKWLGLV+AIWVQSISGNNYTFSNYSAALKSL+NLSQLQLNNLSVAK
Sbjct: 1   MNSFRRSPPSPSALKWLGLVTAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAK 60

Query: 61  DLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMG 120
           DLGKAFGLLAGL+SDRLSTSLLL IGSIEGLIGYGAQWLVVS+KIKPLPYWQMCIFLCMG
Sbjct: 61  DLGKAFGLLAGLASDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMG 120

Query: 121 GNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 180
           GNSTTWMNTGVLVTCLRNFR++RGTVSGILKGYIALSTAIFTD CSALFSDNPSSFLALL
Sbjct: 121 GNSTTWMNTGVLVTCLRNFRKNRGTVSGILKGYIALSTAIFTDFCSALFSDNPSSFLALL 180

Query: 181 SLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS 240
           SLVPF VCL AILFLRE+P SADDETE FKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS
Sbjct: 181 SLVPFAVCLAAILFLREVPRSADDETEEFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS 240

Query: 241 RIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHTIV 300
           RIF I+LLSLL SPLVIPLHSFLKN+GGSGEV EALLAGES AD+AVEGKP IGEDHTIV
Sbjct: 241 RIFCIVLLSLLVSPLVIPLHSFLKNKGGSGEVAEALLAGESGADNAVEGKPVIGEDHTIV 300

Query: 301 EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSG 360
           EAMKT EFWI+FVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSG
Sbjct: 301 EAMKTFEFWIMFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSG 360

Query: 361 SASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVP 420
           SASEHFIKKAGMPRPLWNAASQILMALGYI+LAIAIPGSLYIGSIVVGICYGVRLAITVP
Sbjct: 361 SASEHFIKKAGMPRPLWNAASQILMALGYILLAIAIPGSLYIGSIVVGICYGVRLAITVP 420

Query: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYRL 480
           MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTC GAHCYRL
Sbjct: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCVGAHCYRL 480

Query: 481 VFVVMACSCGIGFAMDLFLAFRTKRLYSKMRALKR 516
           VFVVMACSC IGFAMD FLAFRTKRLYSKMRALKR
Sbjct: 481 VFVVMACSCAIGFAMDSFLAFRTKRLYSKMRALKR 515

BLAST of Pay0003099 vs. NCBI nr
Match: XP_038887463.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 897.9 bits (2319), Expect = 4.1e-257
Identity = 458/513 (89.28%), Postives = 482/513 (93.96%), Query Frame = 0

Query: 1   MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAK 60
           M+IF R PPSSSALKWLGLV+A+WVQSISGNNYTFSNYSAALKSL+NLSQLQLNNLSVAK
Sbjct: 1   MSIFHRPPPSSSALKWLGLVTAVWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAK 60

Query: 61  DLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMG 120
           DLGKAFGLLAG++SDRLSTSLLL IGSIEGLIGYGAQWLVVS+KI PLPYWQMCIFLCMG
Sbjct: 61  DLGKAFGLLAGIASDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKINPLPYWQMCIFLCMG 120

Query: 121 GNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 180
           GNSTTWMNTGVLVTCLRNFRR+RGTVSGILKGYIALSTAIFTD+CSALFSDNPSSFLALL
Sbjct: 121 GNSTTWMNTGVLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDICSALFSDNPSSFLALL 180

Query: 181 SLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS 240
           SLVPF VCLTAILFLREIPCSAD+ETE+ +YFW+LNAVSV VAVTLL FDSIPNP S +S
Sbjct: 181 SLVPFAVCLTAILFLREIPCSADNETEDSRYFWILNAVSVIVAVTLLAFDSIPNPTSSIS 240

Query: 241 RIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHTIV 300
           RIFSI+LL  LASPL IPLHSFLKNRG SGE+ EALLA ES A+  VEG+P IGEDHTIV
Sbjct: 241 RIFSIVLLIFLASPLAIPLHSFLKNRGRSGEIAEALLADESVAEGVVEGQPVIGEDHTIV 300

Query: 301 EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSG 360
           EAMKTVEFWILF SFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSG
Sbjct: 301 EAMKTVEFWILFGSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSG 360

Query: 361 SASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVP 420
           S SEHFI+K GMPRPLWNAASQ+LMALGYI+LAIA+PGSLYIGSI+VGICYGVRLAITVP
Sbjct: 361 SVSEHFIRKDGMPRPLWNAASQVLMALGYILLAIAMPGSLYIGSIIVGICYGVRLAITVP 420

Query: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYRL 480
           MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEAT  K GGN+C G HCYRL
Sbjct: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATRRKGGGNSCVGGHCYRL 480

Query: 481 VFVVMACSCGIGFAMDLFLAFRTKRLYSKMRAL 514
           VFVVMACSC IGFAMDLFLA RTKRLYSKMR L
Sbjct: 481 VFVVMACSCVIGFAMDLFLASRTKRLYSKMRVL 513

BLAST of Pay0003099 vs. NCBI nr
Match: XP_031744364.1 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X2 [Cucumis sativus])

HSP 1 Score: 837.0 bits (2161), Expect = 8.6e-239
Identity = 439/515 (85.24%), Postives = 451/515 (87.57%), Query Frame = 0

Query: 1   MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAK 60
           MN FRRSPPS SALKWLGLV+AIWVQSISGNNYTFSNYSAALKSL+NLSQLQLNNLSVAK
Sbjct: 1   MNSFRRSPPSPSALKWLGLVTAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAK 60

Query: 61  DLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMG 120
           DLGKAFGLLAGL+SDRLSTSLLL IGSIEGLIGYGAQWLVVS+KIKPLPYWQMCIFLCMG
Sbjct: 61  DLGKAFGLLAGLASDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMG 120

Query: 121 GNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 180
           GNSTTWMNTGVLVTCLRNFR++RGTVS                                 
Sbjct: 121 GNSTTWMNTGVLVTCLRNFRKNRGTVS--------------------------------- 180

Query: 181 SLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS 240
                            +P SADDETE FKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS
Sbjct: 181 -----------------VPRSADDETEEFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS 240

Query: 241 RIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHTIV 300
           RIF I+LLSLL SPLVIPLHSFLKN+GGSGEV EALLAGES AD+AVEGKP IGEDHTIV
Sbjct: 241 RIFCIVLLSLLVSPLVIPLHSFLKNKGGSGEVAEALLAGESGADNAVEGKPVIGEDHTIV 300

Query: 301 EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSG 360
           EAMKT EFWI+FVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSG
Sbjct: 301 EAMKTFEFWIMFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSG 360

Query: 361 SASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVP 420
           SASEHFIKKAGMPRPLWNAASQILMALGYI+LAIAIPGSLYIGSIVVGICYGVRLAITVP
Sbjct: 361 SASEHFIKKAGMPRPLWNAASQILMALGYILLAIAIPGSLYIGSIVVGICYGVRLAITVP 420

Query: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYRL 480
           MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTC GAHCYRL
Sbjct: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCVGAHCYRL 465

Query: 481 VFVVMACSCGIGFAMDLFLAFRTKRLYSKMRALKR 516
           VFVVMACSC IGFAMD FLAFRTKRLYSKMRALKR
Sbjct: 481 VFVVMACSCAIGFAMDSFLAFRTKRLYSKMRALKR 465

BLAST of Pay0003099 vs. NCBI nr
Match: XP_022989043.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 802.0 bits (2070), Expect = 3.1e-228
Identity = 411/512 (80.27%), Postives = 456/512 (89.06%), Query Frame = 0

Query: 1   MNIFR-RSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVA 60
           M+IF    PPSSSA+KWL LVSA+WVQSI+GNNYTFSNYSAALKSL+NLSQLQLNNLSVA
Sbjct: 1   MSIFHPPPPPSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVA 60

Query: 61  KDLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCM 120
           KDLGKAFGLL+GL+SDRL TSLLL IGS+EGLIGYG QWLVVS+ I PLPYWQ+ IF+CM
Sbjct: 61  KDLGKAFGLLSGLASDRLPTSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCM 120

Query: 121 GGNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLAL 180
           GGNS+TWMNT VLVTCLRNFRR+ G+VSG+LKGY+ALSTAIFTDLC ALFS++PSSFLAL
Sbjct: 121 GGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLAL 180

Query: 181 LSLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFL 240
           L++VPF VCL AILFLRE P + D+ETE+ +YFWVLNA++VAVAV+LL FD IPNP S +
Sbjct: 181 LAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDLIPNPTSSV 240

Query: 241 SRIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHTI 300
           S IFSI+LL LL SPLVIP+HSFLKNRG SG   EALLA E  A+    G P IGEDHTI
Sbjct: 241 SIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHTI 300

Query: 301 VEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILS 360
           VEAMKTVEFW+LFVSFL GVGTGLAVMNNMGQIGLALGYDDVS FISLMSIWGFFGRILS
Sbjct: 301 VEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRILS 360

Query: 361 GSASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITV 420
           GSASE+FIKKAGM RPLWNAASQILMA+GYI+LAI +P SLYIGSI+VGICYGVRLAI+V
Sbjct: 361 GSASEYFIKKAGMARPLWNAASQILMAIGYILLAIGMPSSLYIGSIIVGICYGVRLAISV 420

Query: 421 PMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYR 480
           P+ASE+FGLK+YGMIYNVLILNLPLGSFLFSGLLAGILYDMEAT  + GGNTC GAHCYR
Sbjct: 421 PIASEIFGLKDYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATKIEEGGNTCMGAHCYR 480

Query: 481 LVFVVMACSCGIGFAMDLFLAFRTKRLYSKMR 512
           LVFVVMACSC +G  +DL L  +TKRLYSK R
Sbjct: 481 LVFVVMACSCVVGLGVDLLLFIKTKRLYSKTR 512

BLAST of Pay0003099 vs. TAIR 10
Match: AT2G16660.1 (Major facilitator superfamily protein )

HSP 1 Score: 708.4 bits (1827), Expect = 4.3e-204
Identity = 366/535 (68.41%), Postives = 432/535 (80.75%), Query Frame = 0

Query: 4   FRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAKDLG 63
           F+RS  SSSALKWLG V+A+WVQSISGNNYTFSNYS ALKSL+NL+QL+LNNLSVAKD+G
Sbjct: 3   FQRSISSSSALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLNQLELNNLSVAKDVG 62

Query: 64  KAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMGGNS 123
           KAFG+LAGL+SDRL T ++L IG  EGL+GYG QWLVVSR I+P+PYWQMCIFLCMGGNS
Sbjct: 63  KAFGILAGLASDRLPTPVILLIGCFEGLLGYGVQWLVVSRTIQPIPYWQMCIFLCMGGNS 122

Query: 124 TTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALLSLV 183
           TTWMNT VLVTC+RNFRR+RG VSGILKGY+ LSTAIFTDLC+ALFS++P+SFL LL++V
Sbjct: 123 TTWMNTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCTALFSNDPASFLVLLAVV 182

Query: 184 PFIVCLTAILFLREIP--CSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLSR 243
           PF VCLTA+ FLREIP   SA +E E  +YF + N V+V VAV L  +D I       S 
Sbjct: 183 PFAVCLTAVFFLREIPPASSAAEENEETRYFTIFNIVAVVVAVYLQSYDIIGIKTGVFSV 242

Query: 244 IFSIILLSLLASPLVIPLHSFLK--NRGG----SGEVGEALLAGESAA------------ 303
            F+ ILL LLASP+ IP HSF+K  N G      G + E LL  E AA            
Sbjct: 243 AFASILLFLLASPIAIPFHSFIKSLNYGEQDDLEGRIQEPLLRSEIAAAEKEVIVVAAVA 302

Query: 304 ---DDAVEGKPAIGEDHTIVEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDD 363
              ++    KP +GEDHTI+EA+ TV+FW+LFVSFLCGVGTGLAVMNNMGQIGLALGY +
Sbjct: 303 AEEEEKKRKKPVLGEDHTIMEAVLTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYTN 362

Query: 364 VSIFISLMSIWGFFGRILSGSASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSL 423
           VSIF+S+ SIWGFFGRILSG+ SE+F+KKAG PRPLWNAASQILMA+GYI++A+A+P SL
Sbjct: 363 VSIFVSMTSIWGFFGRILSGTLSEYFLKKAGTPRPLWNAASQILMAVGYILMALAVPNSL 422

Query: 424 YIGSIVVGICYGVRLAITVPMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDM 483
           YIGS+VVG+CYGVRLAITVP ASE+FGLK YG+IYN+L+LNLPLGSFLFSGLLAG LYD 
Sbjct: 423 YIGSMVVGVCYGVRLAITVPTASELFGLKYYGLIYNILVLNLPLGSFLFSGLLAGFLYDA 482

Query: 484 EATTTKTGGNTCTGAHCYRLVFVVMACSCGIGFAMDLFLAFRTKRLYSKMRALKR 516
           EAT T  GGNTC GAHCYRL+F+VMA +  IG  +DL LA+RTK +Y+K+ A K+
Sbjct: 483 EATPTPGGGNTCVGAHCYRLIFIVMALASVIGVGLDLVLAYRTKEIYAKIHASKK 537

BLAST of Pay0003099 vs. TAIR 10
Match: AT4G34950.1 (Major facilitator superfamily protein )

HSP 1 Score: 697.6 bits (1799), Expect = 7.6e-201
Identity = 359/551 (65.15%), Postives = 434/551 (78.77%), Query Frame = 0

Query: 7   SPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAKDLGKAF 66
           S  SSSALKWLG V+A+WVQSISGNNYTFSNYS ALKSL+NL+QL+LN+LSVAKD+GKAF
Sbjct: 8   SSSSSSALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLTQLELNSLSVAKDVGKAF 67

Query: 67  GLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMGGNSTTW 126
           G+LAGL+SDRLST ++L IGS EGL+GYG QWLVVSR I+P+PYWQMC+FLCMGGNSTTW
Sbjct: 68  GILAGLASDRLSTPVILLIGSFEGLLGYGVQWLVVSRTIQPIPYWQMCVFLCMGGNSTTW 127

Query: 127 MNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALLSLVPFI 186
           MNT VLVTC+RNFRR+RG VSGILKGY+ LSTAIFTDLC+ALFS +P+SFL LLS+VPF 
Sbjct: 128 MNTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCNALFSSDPASFLVLLSVVPFA 187

Query: 187 VCLTAILFLREIPCSAD--DETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLSRIFS 246
           VCLTA+ FLREIP S    ++ E  KYF V N V+V VAV L  +D I       S  F+
Sbjct: 188 VCLTAVFFLREIPPSTTFAEDNEESKYFAVFNIVAVVVAVYLQSYDIIGIKTGAFSIAFA 247

Query: 247 IILLSLLASPLVIPLHSFLKNR------------------GGSGEVGEALLAGESAADDA 306
            ILL LLASP+ +P H+F++++                  G   EV E ++   +AAD+ 
Sbjct: 248 SILLILLASPVAVPFHAFIRSKVHDEQDVEGRIDEPLLRSGSEIEVEETIVGAAAAADNE 307

Query: 307 V----------------------EGKPAIGEDHTIVEAMKTVEFWILFVSFLCGVGTGLA 366
           +                      + +P +GE+HTI+EAM TV+FW+LFVSFLCGVGTGLA
Sbjct: 308 LPPSLKPLSNEEEENHGTIVTTEKKRPVLGEEHTIMEAMLTVDFWVLFVSFLCGVGTGLA 367

Query: 367 VMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSGSASEHFIKKAGMPRPLWNAASQIL 426
           VMNNMGQIGLALGY DVSIF+S+ SIWGFFGRILSG+ SEHFIKKAG PRPLWNAA+QI+
Sbjct: 368 VMNNMGQIGLALGYTDVSIFVSMTSIWGFFGRILSGTISEHFIKKAGTPRPLWNAAAQII 427

Query: 427 MALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVPMASEVFGLKNYGMIYNVLILNLPL 486
           MA+GY+++A+A+PGSLYIGS+VVG+CYGVRLAITVP ASE+FGLK YG+IYN+LILN+PL
Sbjct: 428 MAVGYLLMALALPGSLYIGSMVVGVCYGVRLAITVPTASELFGLKYYGLIYNILILNMPL 487

Query: 487 GSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYRLVFVVMACSCGIGFAMDLFLAFRTK 516
           GSFLFSGLLAG+LYD EAT T  GGNTC GAHC+R+VF+VMA +  IG  +DL LA+RTK
Sbjct: 488 GSFLFSGLLAGLLYDAEATPTPGGGNTCVGAHCFRIVFIVMAFASIIGVGLDLLLAYRTK 547

BLAST of Pay0003099 vs. TAIR 10
Match: AT1G80530.1 (Major facilitator superfamily protein )

HSP 1 Score: 311.2 bits (796), Expect = 1.5e-84
Identity = 188/544 (34.56%), Postives = 293/544 (53.86%), Query Frame = 0

Query: 10  SSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAKDLGKAFGLL 69
           S S   W+GL +A WVQ  +G+  TF  YS+ALKS+L  SQ Q+  L VA DLG+  GLL
Sbjct: 8   SGSRPPWVGLAAAAWVQVSAGSGSTFPLYSSALKSVLGFSQQQVTILGVACDLGENMGLL 67

Query: 70  AGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMGGNSTTWMNT 129
            G +S++L    +L IG+    +G+G  WL VS+ +  LP+W + + L +  NS +W  T
Sbjct: 68  PGYASNKLPPWSMLLIGASSCFLGFGVLWLSVSQIVLGLPFWLLFVALALATNSNSWFGT 127

Query: 130 GVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALLSLVPFIVCL 189
             LVT +RNF   RG V+G+LKGYI +S A FT L S +   +    L  L++   ++CL
Sbjct: 128 ASLVTNMRNFPMSRGPVAGLLKGYIGISGAAFTVLFSMVLHHSAMDLLLFLTVGIPVICL 187

Query: 190 TAILFLRE-IPCSADDETE--NFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLSRIFSII 249
           T + F+R  IP + +D +E   F +  V + +  A  V   V   +    S L  +   I
Sbjct: 188 TVMYFIRPCIPATGEDPSEPMYFAFLLVTSILFAAYLVVTTVLSEVFILPSILKYVLVAI 247

Query: 250 LLSLLASPLVIPLHSFL-----------------KNRG---------------------G 309
           ++ LL SPL +P+   L                 K  G                     G
Sbjct: 248 MVLLLLSPLAVPIKMTLFRSNAKSSPLGSSDNLAKEEGTHEEPLLTPSTSASNLGPIFEG 307

Query: 310 SGEVGEALLAGESAADDAVEGKPAIGEDHTIVEAMKTVEFWILFVSFLCGVGTGLAVMNN 369
             E    +L  E+      + KP  GED    +     +FW+L+  +  G+G+G+ V NN
Sbjct: 308 DDESDMEILLAEAEGAVKKKRKPRRGEDFKFGQVFVKADFWLLWFVYFLGMGSGVTVSNN 367

Query: 370 MGQIGLALGYDDVSIFISLMSIWGFFGRILSGSASEHFIKKAGMPRPLWNAASQILMALG 429
           + QIG A G  D +I + L S + F GR+ SG+ SEHF++   +PR LW  A+Q++M   
Sbjct: 368 LAQIGFAFGIKDTTILLCLFSFFNFIGRLASGAISEHFVRSRTLPRTLWMGAAQLVMVFT 427

Query: 430 YIVLAIAIPGSLYIGSIVVGICYGVRLAITVPMASEVFGLKNYGMIYNVLILNLPLGSFL 489
           +++ A+AI  ++Y+ + ++GIC G +  +++   SE+FGL+++G+ +N ++L  PLG+ +
Sbjct: 428 FLLFAMAIDHTIYVATALIGICMGFQF-LSIATISELFGLRHFGINFNFILLGNPLGATI 487

Query: 490 FSGLLAGILYDMEATTTKTGGNTCTGAHCYRLVFVVMACSCGIGFAMDLFLAFRTKRLYS 513
           FS +LAG +YD EA   K G  TC G  C+R+ F+V+A  CG+G  + + L  R + +Y 
Sbjct: 488 FSAILAGYIYDKEA--DKQGKMTCIGPDCFRVTFLVLAGVCGLGTLLSIILTVRIRPVYQ 547

BLAST of Pay0003099 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 305.8 bits (782), Expect = 6.4e-83
Identity = 190/564 (33.69%), Postives = 302/564 (53.55%), Query Frame = 0

Query: 15  KWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAKDLGKAFGLLAGLSS 74
           +WL  V+A+W+QS +G  Y F + S  +KS LN +Q QL+ L VAKDLG + G LAG  S
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVGFLAGTLS 75

Query: 75  DRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMGGNSTTWMNTGVLVT 134
           + L     L +GS++ L+GYG  WL+V+ +   LP W MCI + +G N  T+ NT  LV+
Sbjct: 76  EILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYFNTAALVS 135

Query: 135 CLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALLSLVPFIVCLTAILF 194
            ++NF + RG V GILKG+  L  AI + + + + S + +S + ++++ P +V +  + F
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPSVVVVPLMFF 195

Query: 195 LREIPCSADDETENFKYFWVLNAVSVAVA----VTLLVFDSIPNPNSFLSRIFSIILLSL 254
           +R +       + +   F V+ AV + +A      +LV D I   +S +   F+++L ++
Sbjct: 196 IRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIII-AFTVVLFAI 255

Query: 255 LASPLVIPLHS--FLKNRGGSGEVGEALLAG----------------------------- 314
           L  P+ IP+ +  F  +      + E LL                               
Sbjct: 256 LLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSEVEDEKPK 315

Query: 315 ----------------------ESAADDAVEGK----PAIGEDHTIVEAMKTVEFWILFV 374
                                 ++AA+ AV  K    P  GED T+ +A+   +FW++F 
Sbjct: 316 EVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKADFWLIFF 375

Query: 375 SFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSGSASEHFIKKAGMP 434
           S L G G+GL V++N+GQ+  +LGYD+  +F+S++SIW F GRI  G  SE  ++    P
Sbjct: 376 SLLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWNFLGRIGGGYFSELIVRDYAYP 435

Query: 435 RPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVPMASEVFGLKNYGM 494
           RP+  A +Q++M++G+I  A   PG+++IG++++G+ YG   AI    ASE+FGLK +G 
Sbjct: 436 RPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASELFGLKKFGA 495

Query: 495 IYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGG-------NTCTGAHCYRLVFVVMA 511
           +YN L L  P GS +FSGL+A  +YD EA     G          C G+ CY L  ++M+
Sbjct: 496 LYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPDDVLRCRGSICYFLTSLIMS 555

BLAST of Pay0003099 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 301.6 bits (771), Expect = 1.2e-81
Identity = 177/558 (31.72%), Postives = 300/558 (53.76%), Query Frame = 0

Query: 15  KWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAKDLGKAFGLLAGLSS 74
           +WL  V+A+W+QS +G  Y F + S  +KS LN +Q +L+ L VAKDLG + G +AG  S
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLS 75

Query: 75  DRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMGGNSTTWMNTGVLVT 134
           + L     L +G+++ LIGYG  WL+V+ +   LP W MC+ + +G N  T+ NTG LV+
Sbjct: 76  EILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVS 135

Query: 135 CLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALLSLVPFIVCLTAILF 194
            ++NF + RG V GILKG+  L  AI + + + + S NP+S + ++++ P +V +  + F
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCLMFF 195

Query: 195 LREIPCSAD---DETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLSRIFSIILLSLL 254
           +R +         +  +F + + +  +  A  +++++   +   +  +  +F+I+L  +L
Sbjct: 196 IRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLFVIL 255

Query: 255 ASPLVIPLHS--FLKNRGGSGEVGEALLAG------------------------------ 314
             P+++P+ +  F +       + E L+                                
Sbjct: 256 VVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVDLLP 315

Query: 315 ----------------ESAADDAVEGK----PAIGEDHTIVEAMKTVEFWILFVSFLCGV 374
                           ++AA+ AV       P  GED T+ +A+   +FW++F S L G 
Sbjct: 316 ASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLLLGS 375

Query: 375 GTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSGSASEHFIKKAGMPRPLWNA 434
           G+GL V++N+GQ+  +LGYD+  + +S++SIW F GRI  G  SE  ++    PRP+  A
Sbjct: 376 GSGLTVIDNLGQMSQSLGYDNTHVLVSMISIWNFLGRIGGGYFSELVVRDYAYPRPVAMA 435

Query: 435 ASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVPMASEVFGLKNYGMIYNVLI 494
            +Q++M++G+I  A   PG++YIG++++G+ YG   AI    ASE+FGLK +G +YN L 
Sbjct: 436 VAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGALYNFLT 495

Query: 495 LNLPLGSFLFSGLLAGILYDMEATTTKTG-------GNTCTGAHCYRLVFVVMACSCGIG 511
           L  P GS +FSG++A  +YD EA     G          C G+ C+ L  ++M+  C I 
Sbjct: 496 LANPAGSLVFSGMIASSIYDREAERQAHGSVFDPDDALRCNGSICFFLTSLIMSGFCIIA 555