Pay0002705 (gene) Melon (Payzawat) v1

Overview
NamePay0002705
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionRNA pseudouridine synthase 4, mitochondrial
Locationchr01: 621747 .. 625839 (+)
RNA-Seq ExpressionPay0002705
SyntenyPay0002705
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAACAAAAATTTAAATTCGACCTATATAATATGAATTGAATAGTCAGGATCATCGAGTTATTCGAGTTGGACTTACCACCCCTACTTTCCCATCACTTCTTAAAACACTTACCATTTTTCATTTTGCCATCCGTTAGAAATCAGCCGGACGTCAAGATGGGCCATATCCAATATATATGTGTTGGCCCATTTTCTTGTTTTTGGTCCAAATACAAAACCCCTCAATTTCCTCTACCTCCGGCGCCACCCACCATGGTACTCCCGCCGCTCTTCCGCCCTTCCGTCATTCTCCGTACTTCCCCGTCTTACACTGATCCCAGCAGCGAAATTCTCTATTTCTCAATTTTTCATGTGCTGAAGTCCACCTTAGCCTTGAGTAACGCCTCCGAACCCGTATCCCAACAGAAGAATGAGAAGGGAAGCAATAGATGGTTCACGCTGCCTCCATACACTGCGACTGTAAATGCTTCGATTTTAGGTAATCGCATACTGAACAGAGGAGCTCAAGCCGAAGTAGAAACTTCCACCGCTGCAATAACTGCTCTGAAATGGGTGATTCATTGCTGCCCCGAATTGCCGAGGAGCCTCGTGCAAAAGCTATTTCGACTGAGAAAGGTTCCGTCCTCAATTTCACTTAATACTATGTTGAATTCTCGCAATTGAATAATCAGTTAAGCTAACTTAAATGATGTCTTTTGATCTTTCTTCAATTGCTTGCCATAGCAATAGCATGCAATTGAATGTTAGTGTGGCAATTCGCATCGTCAGATTTCGACTTTATTGTTAAATTGAAGCATCTTCTTTAGTTTATGAATGTTTTCCTTTTGATGTCTTTCAGGTTCGAAGAGGTTCTTCTATGATTGAAAATTCTGAATATAATTTGGAAGCTCGAGACCAACTTAAAAGAGTGAGCCGCTATCTTCTATACCTACAGAGATATTAATTTCTCTATTTATATTTATATAACATTTGATTATCATGTTTTCCACATTGAAGGAAACTTCCTTAGATCTTTACTAACATGTTTAAGTTATTGCAAGGTGGCAGCCAAGGACATTCTGAACGTAGGTGATCAAATTTTTCTCCCTATCTCCGCTCAAGTATTCTCTACAGAGAAAACTCGGGATTATTGCAATAAAGACGAAGAGACATTCATGCATAGTCTAGTATTATACAAGGTTTTCACTTTTCGCTGTTGTTTTTTGTTGCCTGGAGGAAGGTGGGGTTAAGAACTCTTAGAGGAAGGTGGGGTTAAGAACACTTAGAGGAAGGTGGGTTTAAGAACACTTAGAGGTACAGGTAATTGGGGCACTAAGTTGATTATTATAGTTTGTGGGTTATAACAGCCTGATTTTGAGATGCAAACTATTTTAGTTTGTGTTTTAATTGTCTCTGTTTGAATGCAATCTATTTCAGTTTGAGTAGGAAATGGTATGTACGGTAACAAAGAGAGAAAGAGAGGATGAGTAGGAAATAGTAGATATTGTAACAAACATGAAACACCCCTATGTTCCTCTTGCTGCTAGTGACACATTTTCTTTTTTCTTTTTTTGGTATAAGGATCCAGCAATACTTGTTATCAATAAACCTCCCGGCTTGCCTGTACAGGTATTGTATTCAACTTGAACCATTAATCGATCCTTTTGAATGATCATGGTCTTAGTTTCCAAAATTCCAGTAAATAAAAGTGTTTCTGCAGGGTGGTATTGGTGTGAAAAGAAGTTTAGATGAATTAGCTGCAAGTTGTTTAAGTTATGGCTACTCAGAACCACCTCGACTAGTAAGTTTTTCTTTAAATGATAACCTCTGACTTCGTGAGTTTGTTTAACTACATTAAATAGCATGCCATATAAGGATTTCAAAAAAGGCATTTGTAGTGTCTTTCTAGTTTGATGTTTTTCCTTTTATGATGAACGTGTATTGCAATCTTTATCTTGTTACAAACAATGATATGGGCTTTGCTTGTTGCTAGGTACACAGGCTTGACAGGGACAGCAGTGGCATCTTGATAATGGGCAGGACTTCCACAAGTACTTCACTTCTTCATTCTGTCTTCCGTGAGAAAACTTTTGATGCATCAAAACATGTAGTTCTCTCTGTACTCATATTTTCTATTAAGCAAAATGAAAATCTGATAGAGATAAAAACACATATAAAGCATCGTTATTGTTCAAACTGACATGATTAATTCTTTTACTGTTCATTGCATTGAAGGCAAACCATGTTGATAGTGAAAAAAAGATTTTGCTAAAAAGGTATTGGGCACTTGTAATTGGTTCTCCTCGACGTTCAAAGGGGTTAATTACAGCTCCACTAGGAAAGGTTTGTGTATAATTTCTTTCAAGCAACTATCTTGGAAGTTTTATCCTATTCTAAGTAAACATACATTTGCATTGGTTTCTTTGTGAGTAATTATCTGTATTTTTTCCTTATATTTTCTCTTGTAATTTAGTGTGCTTCTGATGATTTTAGATTGTCTTGGATGATGGAAGATCAGAGCGAATTATGGTCATTGATAATGCCGAACCTATGCCTTTTCAGCAAGCAATTACTAGATATAAAGTGATCAAATCTCATCATGGTTTGCACTTCACCCTTTCTAAATGAAGCATTTAACGTTTCATGCTGATTTTTCGAGCAATAGACATAATTTTAGTCCAAATATTGAAAGCACATTGGAACGTCGTAGGAACATGGATTAAACTGTTTACTTTGTAGGTTTTTCATTTTTTAGATTACTTGGGCAGCTTGTATTAGCCATTAGGCACATGAGATCATCTCTTGATCTTGTTTCTAATAAAGTTTGCATCTGATCATTCGATTAGGAGGGGACTTTTTTTTATAATCGAGAGAGTAGAATATAACTCAGAACTTGTATACATTGTTATTCTTCCTTTTTAGTTCAGAATGAGAAAATTTGAATGCCTCTTAAATGGCTAGAATGCTTTATTTTCTAGTAGTTATATATAATTCTACTTGCGTGACTTTTGATAGAAAGTCTCACAAGAAAAAGAAAGAGCACTTTGCATGCCAAGAATTTACCGTTTTAAAATAAAGCATTTCGGTCAAAATTAATTAATTCCTTTATAACTCCAAAGCGCATATCTTATCTATGCTATAAGATAAAATATCAGATTCTGTTCTCTTATTGCATGGCAATTGTTCAGGATACACTTGGTTGGAGTTGTGTCCCCTCACTGGAAGAAAACATCAGGTACCATCCAGCACACCATAGCTCCAACCACTACAAGTATCCATCTCGTTTTCTACAGATTTTTGGCTTAATATACCTTTATTGTATGTGTTCAATGCAGCTTCGTGTACACTGTGCCGAGGTATTAGGAACGCCTATAGTTGGAGACTACAAATATGGATGGCAAGCACATAAAAATTGGAAACCATTTCATTATAATTTCCACAAGAATCAAGGAAAGAAACAATTCCTCCCTTTTGGTCTTGACTTTGAAAGTGGAAGTATTGCAGAAACTAATCCTCGTCTACACCTTCATTGTAAGCAACTGACTTTACCAGATGTTTCTTTTGCACTGCATAATGATTCTCTAGATCAGGACTGCTCAAGATTGGAATGTCTGGAGTTGGATGCTCCTTTGCCTTCATACATGCAAAAAAGTTGGGATATTATAAATAGTTAGACTGCCATCAAAATTTTTGGACCAACCACTCATCGTTTTGGTCTTCATTTTCCAAGTGGAAATACTGTGGTAAACAACCCTGCCTAAACTTTCAACCCTAGCAATTTCTTTTATCTGAAGGACTTGGGTTGATAGACTGATATCGTAAGTCATCAAATTGTCATTCATGCTAGACACTTTTATAGATTAAATATTTATTAGTGGTGTTTGATAAGAGTTGGATTAAGTTTGTAATAATTGTTTTTGTTGATATTGTTTGGTGTACAAAGTTCAGTTGAGTTAGCTTAGAAAATCTATGGTTGGGGTTTTGAGTTGAGTTGAGTTATGAGTATCTGGTGCAGTTTTGTGTACCCTAAAAAGTATGTATTTTTATAGATTGTTTTACCATCAATTAACCACTTATTTGAATGTGAACTCAATAAATAAAGATATATTTT

mRNA sequence

AAAAAACAAAAATTTAAATTCGACCTATATAATATGAATTGAATAGTCAGGATCATCGAGTTATTCGAGTTGGACTTACCACCCCTACTTTCCCATCACTTCTTAAAACACTTACCATTTTTCATTTTGCCATCCGTTAGAAATCAGCCGGACGTCAAGATGGGCCATATCCAATATATATGTGTTGGCCCATTTTCTTGTTTTTGGTCCAAATACAAAACCCCTCAATTTCCTCTACCTCCGGCGCCACCCACCATGGTACTCCCGCCGCTCTTCCGCCCTTCCGTCATTCTCCGTACTTCCCCGTCTTACACTGATCCCAGCAGCGAAATTCTCTATTTCTCAATTTTTCATGTGCTGAAGTCCACCTTAGCCTTGAGTAACGCCTCCGAACCCGTATCCCAACAGAAGAATGAGAAGGGAAGCAATAGATGGTTCACGCTGCCTCCATACACTGCGACTGTAAATGCTTCGATTTTAGGTAATCGCATACTGAACAGAGGAGCTCAAGCCGAAGTAGAAACTTCCACCGCTGCAATAACTGCTCTGAAATGGGTGATTCATTGCTGCCCCGAATTGCCGAGGAGCCTCGTGCAAAAGCTATTTCGACTGAGAAAGGTTCGAAGAGGTTCTTCTATGATTGAAAATTCTGAATATAATTTGGAAGCTCGAGACCAACTTAAAAGAGTGGCAGCCAAGGACATTCTGAACGTAGGTGATCAAATTTTTCTCCCTATCTCCGCTCAAGTATTCTCTACAGAGAAAACTCGGGATTATTGCAATAAAGACGAAGAGACATTCATGCATAGTCTAGTATTATACAAGGATCCAGCAATACTTGTTATCAATAAACCTCCCGGCTTGCCTGTACAGGGTGGTATTGGTGTGAAAAGAAGTTTAGATGAATTAGCTGCAAGTTGTTTAAGTTATGGCTACTCAGAACCACCTCGACTAGTACACAGGCTTGACAGGGACAGCAGTGGCATCTTGATAATGGGCAGGACTTCCACAAGTACTTCACTTCTTCATTCTGTCTTCCGTGAGAAAACTTTTGATGCATCAAAACATGCAAACCATGTTGATAGTGAAAAAAAGATTTTGCTAAAAAGGTATTGGGCACTTGTAATTGGTTCTCCTCGACGTTCAAAGGGGTTAATTACAGCTCCACTAGGAAAGATTGTCTTGGATGATGGAAGATCAGAGCGAATTATGGTCATTGATAATGCCGAACCTATGCCTTTTCAGCAAGCAATTACTAGATATAAAGTGATCAAATCTCATCATGGATACACTTGGTTGGAGTTGTGTCCCCTCACTGGAAGAAAACATCAGCTTCGTGTACACTGTGCCGAGGTATTAGGAACGCCTATAGTTGGAGACTACAAATATGGATGGCAAGCACATAAAAATTGGAAACCATTTCATTATAATTTCCACAAGAATCAAGGAAAGAAACAATTCCTCCCTTTTGGTCTTGACTTTGAAAGTGGAAGTATTGCAGAAACTAATCCTCGTCTACACCTTCATTGTAAGCAACTGACTTTACCAGATGTTTCTTTTGCACTGCATAATGATTCTCTAGATCAGGACTGCTCAAGATTGGAATGTCTGGAGTTGGATGCTCCTTTGCCTTCATACATGCAAAAAAGTTGGGATATTATAAATAGTTAGACTGCCATCAAAATTTTTGGACCAACCACTCATCGTTTTGGTCTTCATTTTCCAAGTGGAAATACTGTGGTAAACAACCCTGCCTAAACTTTCAACCCTAGCAATTTCTTTTATCTGAAGGACTTGGGTTGATAGACTGATATCGTAAGTCATCAAATTGTCATTCATGCTAGACACTTTTATAGATTAAATATTTATTAGTGGTGTTTGATAAGAGTTGGATTAAGTTTGTAATAATTGTTTTTGTTGATATTGTTTGGTGTACAAAGTTCAGTTGAGTTAGCTTAGAAAATCTATGGTTGGGGTTTTGAGTTGAGTTGAGTTATGAGTATCTGGTGCAGTTTTGTGTACCCTAAAAAGTATGTATTTTTATAGATTGTTTTACCATCAATTAACCACTTATTTGAATGTGAACTCAATAAATAAAGATATATTTT

Coding sequence (CDS)

ATGGGCCATATCCAATATATATGTGTTGGCCCATTTTCTTGTTTTTGGTCCAAATACAAAACCCCTCAATTTCCTCTACCTCCGGCGCCACCCACCATGGTACTCCCGCCGCTCTTCCGCCCTTCCGTCATTCTCCGTACTTCCCCGTCTTACACTGATCCCAGCAGCGAAATTCTCTATTTCTCAATTTTTCATGTGCTGAAGTCCACCTTAGCCTTGAGTAACGCCTCCGAACCCGTATCCCAACAGAAGAATGAGAAGGGAAGCAATAGATGGTTCACGCTGCCTCCATACACTGCGACTGTAAATGCTTCGATTTTAGGTAATCGCATACTGAACAGAGGAGCTCAAGCCGAAGTAGAAACTTCCACCGCTGCAATAACTGCTCTGAAATGGGTGATTCATTGCTGCCCCGAATTGCCGAGGAGCCTCGTGCAAAAGCTATTTCGACTGAGAAAGGTTCGAAGAGGTTCTTCTATGATTGAAAATTCTGAATATAATTTGGAAGCTCGAGACCAACTTAAAAGAGTGGCAGCCAAGGACATTCTGAACGTAGGTGATCAAATTTTTCTCCCTATCTCCGCTCAAGTATTCTCTACAGAGAAAACTCGGGATTATTGCAATAAAGACGAAGAGACATTCATGCATAGTCTAGTATTATACAAGGATCCAGCAATACTTGTTATCAATAAACCTCCCGGCTTGCCTGTACAGGGTGGTATTGGTGTGAAAAGAAGTTTAGATGAATTAGCTGCAAGTTGTTTAAGTTATGGCTACTCAGAACCACCTCGACTAGTACACAGGCTTGACAGGGACAGCAGTGGCATCTTGATAATGGGCAGGACTTCCACAAGTACTTCACTTCTTCATTCTGTCTTCCGTGAGAAAACTTTTGATGCATCAAAACATGCAAACCATGTTGATAGTGAAAAAAAGATTTTGCTAAAAAGGTATTGGGCACTTGTAATTGGTTCTCCTCGACGTTCAAAGGGGTTAATTACAGCTCCACTAGGAAAGATTGTCTTGGATGATGGAAGATCAGAGCGAATTATGGTCATTGATAATGCCGAACCTATGCCTTTTCAGCAAGCAATTACTAGATATAAAGTGATCAAATCTCATCATGGATACACTTGGTTGGAGTTGTGTCCCCTCACTGGAAGAAAACATCAGCTTCGTGTACACTGTGCCGAGGTATTAGGAACGCCTATAGTTGGAGACTACAAATATGGATGGCAAGCACATAAAAATTGGAAACCATTTCATTATAATTTCCACAAGAATCAAGGAAAGAAACAATTCCTCCCTTTTGGTCTTGACTTTGAAAGTGGAAGTATTGCAGAAACTAATCCTCGTCTACACCTTCATTGTAAGCAACTGACTTTACCAGATGTTTCTTTTGCACTGCATAATGATTCTCTAGATCAGGACTGCTCAAGATTGGAATGTCTGGAGTTGGATGCTCCTTTGCCTTCATACATGCAAAAAAGTTGGGATATTATAAATAGTTAG

Protein sequence

MGHIQYICVGPFSCFWSKYKTPQFPLPPAPPTMVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRWFTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLRKVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRDSSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPRLHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS
Homology
BLAST of Pay0002705 vs. ExPASy Swiss-Prot
Match: Q9LT72 (RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g19440 PE=2 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 4.9e-125
Identity = 233/441 (52.83%), Postives = 303/441 (68.71%), Query Frame = 0

Query: 72  ALSNASEPVSQQKNEKGSNRWFTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALK 131
           A   ++   S  + +    +W TLPP++ T++ + +G  +L+ G   +  T  +  TAL+
Sbjct: 35  AAHQSTRSYSTTQTDDSRGKWLTLPPFSPTIDGTAVGKDLLSDGDSVKSSTDNSKTTALR 94

Query: 132 WVIHCCPELPRSLVQKLFRLRKVRRGSSM-IENSEYNLEARDQLKRVAAKDILNVGDQIF 191
           W++ C P+LPR+LVQKLFRLR+VRR  SM ++  E     R QLKRVAAK+ LNVGD+I+
Sbjct: 95  WILRCRPDLPRTLVQKLFRLRQVRREMSMSVDGDELQ---RSQLKRVAAKESLNVGDRIY 154

Query: 192 LPISA-----QVFSTEKTRDYCNKDEETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKR 251
           LP+S      Q    +K    C+ +E  F+ SLVLYKDPAI+V+NKP GL VQGG G+K 
Sbjct: 155 LPLSVDNDTPQTPPAKKESFQCSDEERKFVCSLVLYKDPAIIVLNKPHGLAVQGGSGIKT 214

Query: 252 SLDELAASCLSYGYSEPPRLVHRLDRDSSGILIMGRTSTSTSLLHSVFREKTFDASKHAN 311
           S+DELAASCL +  SE PRLVHRLDRD SG+L++ RT T+ ++LHS+FREKT  AS +  
Sbjct: 215 SIDELAASCLKFDKSESPRLVHRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYG- 274

Query: 312 HVDSEKKILLKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAI 371
            V    K L ++Y ALVIG P R +G I+APL K+V+DDG+SERI V DN E +  Q AI
Sbjct: 275 -VKKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAI 334

Query: 372 TRYKVIKSH-HGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYN 431
           T Y+VI+S  HGYTWLEL PLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHK  +PF  +
Sbjct: 335 TEYRVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKAREPF-VS 394

Query: 432 FHKNQGKKQFLPFGLDFESGSIAETNPRLHLHCKQLTLPDVSFALH--NDSLDQDCSRLE 491
              N  K+   PFGLD + G ++   P LHLH KQ+ LP++S  L     S D D S L+
Sbjct: 395 SENNPTKQSSSPFGLDLDGGDVSSKQPHLHLHSKQIDLPNISQLLEKMQVSSDSDISDLD 454

Query: 492 CLELDAPLPSYMQKSWDIINS 504
            L+ DAPLPS+MQ S++++ S
Sbjct: 455 SLKFDAPLPSHMQLSFNLLKS 469

BLAST of Pay0002705 vs. ExPASy Swiss-Prot
Match: Q0J4D4 (RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0520100 PE=2 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 3.7e-80
Identity = 160/395 (40.51%), Postives = 245/395 (62.03%), Query Frame = 0

Query: 127 ITALKWVIHCCPELPRSLVQKLFRLRKVRRGSSMIENSEYNLEA-RDQLKRVAAKDILNV 186
           +TA+ WV H   ++P+ +VQ  F  R V    S  E S   +++ +  LK++   D++  
Sbjct: 79  VTAVSWVKHYLADVPQEVVQAHFNKRLVYSECSDHEVSVETIKSQKHHLKKIKHNDVMEP 138

Query: 187 GDQIFLPISAQVFSTEKTRD-------YCNKDEETFMHSLVLYKDPAILVINKPPGLPVQ 246
           G +I LP+S      +K  +       + NKDE  ++  LV++KD AILV+NKPP +P++
Sbjct: 139 GMRIHLPVSVAEGEIKKRYETIPTATLHPNKDEIEYLRRLVIHKDSAILVLNKPPKVPMK 198

Query: 247 GGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRDSSGILIMGRTSTSTSLLHSVFREKTF 306
           G + V  S+D LAA+ LSYG  E P+LVHRLDR+SSG+L+ GRT  S + LH +F     
Sbjct: 199 GNLPVHNSMDVLAAAALSYGNEEGPKLVHRLDRESSGLLLFGRTKESFTRLHWLFTSVNL 258

Query: 307 DASKHANHVDSEKKILLKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSERIMVIDNAEP 366
            A  ++   ++  +  ++RYWALVIG+P+  +G+I+APL K++LDDG++ER+++   +  
Sbjct: 259 -AKTNSQVWNAACEAYMQRYWALVIGTPKEREGIISAPLSKVLLDDGKAERVILAHPSGI 318

Query: 367 MPFQQAITRYKVI-KSHHGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKN 426
              Q+A+T Y+V+  + HG +W+EL PLTGRKHQLRVHCAE LGTPIVGDYKYGW  H+ 
Sbjct: 319 DGAQEAVTAYRVMGPTIHGCSWIELRPLTGRKHQLRVHCAEALGTPIVGDYKYGWFVHQR 378

Query: 427 WKPF-HYNFHKNQGKKQFL--PFGLDFESGSIAETNPRLHLHCKQLTLPDVSFAL----- 486
           WK     +F    G+   L  P GL+ + GS+    P LHLHC+++ +P+++  L     
Sbjct: 379 WKQNPQPDFEPFTGEPYKLRRPEGLEIQKGSVLSKVPLLHLHCREMVIPNIAKFLSSNGE 438

Query: 487 -HNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 504
            H +       +   L   AP+P++M+ SW+I++S
Sbjct: 439 WHENGAPWSKEKPNLLRFIAPMPAHMKISWNIMSS 472

BLAST of Pay0002705 vs. ExPASy Swiss-Prot
Match: Q5XET6 (RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g78910 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 4.8e-80
Identity = 167/419 (39.86%), Postives = 246/419 (58.71%), Query Frame = 0

Query: 113 NRGAQAEVETSTAAITALKWVIHCCPEL---------PRSLVQKLFRLRKVRRGSSMIEN 172
           N GA  +   S   +TA+ WV +   E+          + LVQ  FR R+        + 
Sbjct: 67  NAGADGDDGDSGGHVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRR--------DA 126

Query: 173 SEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRD-------YCNKDEETFMH 232
           S    +    ++++   +++ +GD+I+LP+S       K  D       Y N DE  ++ 
Sbjct: 127 SREKEDGAIPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQ 186

Query: 233 SLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRDSSGI 292
            LV +KD AI+V+NKPP LPV+G + +  S+D LAA+ LS+G  E PRLVHRLDR++SG+
Sbjct: 187 RLVRFKDSAIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGL 246

Query: 293 LIMGRTSTSTSLLHSVFRE-KTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGLITA 352
           L+MGRT  S   LHSVF + K  ++S  A +   E   + ++YWALVIGSP+  +GLI+A
Sbjct: 247 LVMGRTKESIDYLHSVFSDYKGRNSSCKAWNKACE--AMYQQYWALVIGSPKEKEGLISA 306

Query: 353 PLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVI-KSHHGYTWLELCPLTGRKHQLRV 412
           PL K++LDDG+++R+++   +     Q AIT YKV+    +G +W+EL P+T RKHQLRV
Sbjct: 307 PLSKVLLDDGKTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRV 366

Query: 413 HCAEVLGTPIVGDYKYGWQAHKNWKPF-HYNFHKNQGKKQFL--PFGLDFESGSIAETNP 472
           HCAE LGTPIVGDYKYGW  HK WK     +     GK   L  P GLD + GS+    P
Sbjct: 367 HCAEALGTPIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVP 426

Query: 473 RLHLHCKQLTLPDVSFALHNDSLDQD-------CSRLECLELDAPLPSYMQKSWDIINS 504
            LHLHC+++ LP+++  LH  +  +          + + L   A +PS+M+ SW++++S
Sbjct: 427 LLHLHCREMVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSS 475

BLAST of Pay0002705 vs. ExPASy Swiss-Prot
Match: Q69K07 (RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0103500 PE=2 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 7.2e-76
Identity = 155/312 (49.68%), Postives = 210/312 (67.31%), Query Frame = 0

Query: 83  QKNEKGSNRWFTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPR 142
           +  E+G + W  LPP+ A ++A+    R ++RG   E        TA+KWV  CCP+LP 
Sbjct: 43  ETGERGKSPWLQLPPF-APLDAAAAA-RAISRGG-GEGGDGEQGATAIKWVRRCCPDLPT 102

Query: 143 SLVQKLFRLRKVRRGSSMIENSEYNLEA-RDQLKRVAAKDILNVGDQIFLPISAQVFS-T 202
           SLVQKLFRLRKV++     E S  +  A + +L+RV+AKD L  GD +FLP++ +  S  
Sbjct: 103 SLVQKLFRLRKVKKNVVTAEISSADASAEQHRLRRVSAKDQLMPGDILFLPVNLKESSVA 162

Query: 203 EKTRDYCNKDEETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYS 262
           EKT+ + N++E  F+  L +YKD AI+V+NKPPG+PVQGG+G+K S+D L AS      S
Sbjct: 163 EKTKKFDNRNEINFLRGLEIYKDEAIIVVNKPPGMPVQGGVGIKNSIDVL-ASMFEENSS 222

Query: 263 EPPRLVHRLDRDSSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWA 322
           E PRLVHRLDRD SGIL++GR   STS+LH++FREKT DA       D  + +L ++Y A
Sbjct: 223 EAPRLVHRLDRDCSGILVLGRNQLSTSMLHAIFREKTADAL-----ADGTQHVLQRKYVA 282

Query: 323 LVIGSPRRSKGLITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKS-HHGYTW 382
           LVIG+PR  KGL++APL KI+L DG+SER+ +  ++     Q A+T Y+VI+S   GYTW
Sbjct: 283 LVIGTPRHPKGLLSAPLAKILLQDGKSERLTIRASSNAASVQDALTEYRVIESCPQGYTW 342

Query: 383 LELCPLTGRKHQ 392
           LEL P TGRKHQ
Sbjct: 343 LELFPRTGRKHQ 345

BLAST of Pay0002705 vs. ExPASy Swiss-Prot
Match: Q68XB2 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=rluC PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 4.3e-28
Identity = 89/282 (31.56%), Postives = 150/282 (53.19%), Query Frame = 0

Query: 131 KWVIHCCPELPRSLVQKLFRLRKVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIF 190
           K++    P L + +++K  R ++V   +   + +E NL       RV   D + + D   
Sbjct: 15  KYLKRLYPSLTQGVIEKALRQKQV---TVNFQKAEANL-------RVKEGDTIFINDYFN 74

Query: 191 LPISAQVFSTEKTRDYCNKDEETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDEL 250
           LP++     T    D   K  +  +   ++Y+D  ++ INKP  L  QGG  +  S+D  
Sbjct: 75  LPVTQH--ETLVFADAEIKLAKKILTDYLIYEDDHLIAINKPASLATQGGSKINLSIDS- 134

Query: 251 AASCLSYGYSEPPRLVHRLDRDSSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSE 310
           A   L+Y  ++  +LVHRLD+++SG+L++ +   S+  LH  F+E               
Sbjct: 135 ALKYLNYQGAD-FKLVHRLDKETSGLLLIAKNYLSSVKLHDAFKE--------------- 194

Query: 311 KKILLKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKV 370
            K+++K+Y+A+  G P ++ G++ + +GK     GR  +I  ID+      + AIT YK+
Sbjct: 195 -KLVIKKYFAITYGRPVKNVGIVKSNIGK---SKGRMFKITDIDSDNG---KLAITYYKL 254

Query: 371 IKSHHGYTWL-ELCPLTGRKHQLRVHCAEVLGTPIVGDYKYG 412
           +KS +   +L E  P+TGR HQLR+H A++LG PI+GD KYG
Sbjct: 255 LKSLNNNLFLIEFMPVTGRMHQLRLH-AQLLGCPILGDDKYG 259

BLAST of Pay0002705 vs. ExPASy TrEMBL
Match: A0A5D3C4F5 (RNA pseudouridine synthase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00930 PE=4 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.2e-277
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 33  MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 92
           MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 60

Query: 93  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 152
           FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 120

Query: 153 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 212
           KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE
Sbjct: 121 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 180

Query: 213 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 272
           TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD
Sbjct: 181 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 240

Query: 273 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 332
           SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 300

Query: 333 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 392
           ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 393 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 452
           RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 420

Query: 453 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 504
           LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS
Sbjct: 421 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

BLAST of Pay0002705 vs. ExPASy TrEMBL
Match: A0A1S3BZE7 (RNA pseudouridine synthase 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495275 PE=4 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.2e-277
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 33  MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 92
           MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 60

Query: 93  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 152
           FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 120

Query: 153 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 212
           KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE
Sbjct: 121 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 180

Query: 213 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 272
           TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD
Sbjct: 181 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 240

Query: 273 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 332
           SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 300

Query: 333 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 392
           ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 393 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 452
           RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 420

Query: 453 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 504
           LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS
Sbjct: 421 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

BLAST of Pay0002705 vs. ExPASy TrEMBL
Match: A0A6J1GMP9 (RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111455375 PE=4 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 7.7e-230
Identity = 396/471 (84.08%), Postives = 421/471 (89.38%), Query Frame = 0

Query: 33  MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 92
           MVLP + R SVI R+ PS   P  E L+FSIF   KST ALS A+E VS+QKNEKGSNRW
Sbjct: 1   MVLPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTYALSIATETVSEQKNEKGSNRW 60

Query: 93  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 152
           FTLPP+TAT N S LGNRI+N GAQAE ETST AITALKWV  CCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 153 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 212
           KVRRGSSMIENSE NLEARDQLKRVAA DILN+GD++ LPIS QVF TE+  D CNK+E+
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 213 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 272
            F+HSLVLYKDPAIL INKPPGLPVQGGIGVKRSLD+LAASCLSY YSEPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDRD 240

Query: 273 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 332
           SSGILIMGRTSTSTS+LHS+FREKT  ASKHANHVDSEKKIL KRYWALVIGSPRR+KGL
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDSEKKILQKRYWALVIGSPRRTKGL 300

Query: 333 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 392
           ITAPL K+VLDDGRSE+IMVIDNAE MPFQ AIT YKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLVKMVLDDGRSEQIMVIDNAESMPFQHAITTYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 393 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 452
           RVHCAEVLGTPIVGDYKYGWQAH+NWKPF +N HKNQGKKQ LPFGLDFESGSIAE +PR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENSPR 420

Query: 453 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 504
           LHLHCKQLTLPDVS ALHNDSLD DCSRLE LELDAPLPSYMQ+SWDIIN+
Sbjct: 421 LHLHCKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

BLAST of Pay0002705 vs. ExPASy TrEMBL
Match: A0A6J1D432 (RNA pseudouridine synthase 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111017405 PE=4 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 6.7e-226
Identity = 387/472 (81.99%), Postives = 423/472 (89.62%), Query Frame = 0

Query: 33  MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNE-KGSNR 92
           M+LP L R S+IL  +PS T      L+FS+FH LKST ALSN SE +S++KNE KG NR
Sbjct: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60

Query: 93  WFTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRL 152
           WFTLPPYTATV+ S+LGNRILN+G+QAE ETST AITALKWV+ CCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120

Query: 153 RKVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDE 212
           RK+RRGSSMIE+SE NLEA++QLKRVAAKDILNVGDQIFLPIS QVF  E+ RDYCNK+E
Sbjct: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180

Query: 213 ETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDR 272
           E FMHSLVLYKDPAI+VINKPPGLPVQGGIG+ RSLDELAASCLSY YSEPPRLVHRLDR
Sbjct: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240

Query: 273 DSSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKG 332
           DSSGIL+MGRTSTSTS+LHS+FREKTF ASKH  HVDSEKKIL K+YWALVIGSPRR  G
Sbjct: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKH--HVDSEKKILQKKYWALVIGSPRRPNG 300

Query: 333 LITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQ 392
           LITAPLGKIVLDDGRSE+IMVIDNAE M FQQAIT Y+VIKSHHGYTWLELCPLTGRKHQ
Sbjct: 301 LITAPLGKIVLDDGRSEKIMVIDNAESMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQ 360

Query: 393 LRVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNP 452
           LRVHCAEVLGTPIVGDYKYGWQAH+NW+PF  NFHKNQGKKQ LPFGLDFE+GS+AE +P
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENSP 420

Query: 453 RLHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 504
           RLHLHCKQL LP+VS AL NDSLD D S LECLELDAPLPSYM++SWDIIN+
Sbjct: 421 RLHLHCKQLALPNVSGALPNDSLDHDFSELECLELDAPLPSYMRRSWDIINN 470

BLAST of Pay0002705 vs. ExPASy TrEMBL
Match: A0A6J1IBC5 (RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111470950 PE=4 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 2.0e-225
Identity = 391/472 (82.84%), Postives = 416/472 (88.14%), Query Frame = 0

Query: 33  MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 92
           MVLP L R SVI R+ PS   P  E L+FSIF   KST ALSNA+E VS+QKNEKGSNRW
Sbjct: 1   MVLPALLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSNATETVSEQKNEKGSNRW 60

Query: 93  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 152
           FTLPP+TATVN S LGNRI+N GAQ+E ETST AITALKWV  CCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATVNGSALGNRIMNGGAQSEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 153 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 212
           KVRRGSSMIENSE NLEARDQLKRVAA DILN+GD++ LPIS QVF TE+  D CNK+E+
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 213 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 272
            F+HSLVLYKDPAIL INKPPGLPVQGGIGVKRSLDELAASCLSY YSEPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240

Query: 273 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 332
           SSGILIMGRTSTSTS+LHS+FREKT  ASKHANHVD EK IL KRYWALVIGSP  +KGL
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDREKMILQKRYWALVIGSPSHTKGL 300

Query: 333 ITAPLGKIVLDDGRSERIMVIDNAEP-MPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQ 392
           ITAPL K+VLDDGRSE+IMV+DNAE  MPFQ AIT YKVIKSHHGYTW+ELCPLTGRKHQ
Sbjct: 301 ITAPLVKMVLDDGRSEQIMVVDNAESIMPFQHAITTYKVIKSHHGYTWVELCPLTGRKHQ 360

Query: 393 LRVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNP 452
           LRVHCAE LGTPIVGDYKYGWQAH+N KPF +N HKNQGKKQ LPFGLDFESGSIAE NP
Sbjct: 361 LRVHCAEALGTPIVGDYKYGWQAHRNLKPFPHNSHKNQGKKQSLPFGLDFESGSIAENNP 420

Query: 453 RLHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 504
           RLHLHCK LTLPDVS ALHNDSLD DCSRLE LELDAPLPSYMQ+SWDIIN+
Sbjct: 421 RLHLHCKHLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 472

BLAST of Pay0002705 vs. NCBI nr
Match: XP_008454996.1 (PREDICTED: RNA pseudouridine synthase 4, mitochondrial [Cucumis melo] >KAA0031338.1 RNA pseudouridine synthase 4 [Cucumis melo var. makuwa] >TYK06791.1 RNA pseudouridine synthase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 964.1 bits (2491), Expect = 4.6e-277
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 33  MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 92
           MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 60

Query: 93  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 152
           FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 120

Query: 153 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 212
           KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE
Sbjct: 121 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 180

Query: 213 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 272
           TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD
Sbjct: 181 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 240

Query: 273 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 332
           SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 300

Query: 333 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 392
           ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 393 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 452
           RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 420

Query: 453 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 504
           LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS
Sbjct: 421 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

BLAST of Pay0002705 vs. NCBI nr
Match: XP_011658889.1 (RNA pseudouridine synthase 4, mitochondrial [Cucumis sativus] >KGN43951.2 hypothetical protein Csa_017092 [Cucumis sativus])

HSP 1 Score: 912.5 bits (2357), Expect = 1.6e-261
Identity = 447/471 (94.90%), Postives = 456/471 (96.82%), Query Frame = 0

Query: 33  MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 92
           M L  LFRPSVILRTSPS TDPS EILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW
Sbjct: 1   MGLRALFRPSVILRTSPSCTDPSGEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 60

Query: 93  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 152
           FTLPPYTATVN SILGNRI+N G QAE+  STAAITALKWVI CCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNGSILGNRIMNGGGQAELGASTAAITALKWVIRCCPELPRSLVQKLFRLR 120

Query: 153 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 212
           KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLP+S QVFSTEKTRDYCNK+EE
Sbjct: 121 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEEE 180

Query: 213 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 272
           TFMHSLVL+KDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD
Sbjct: 181 TFMHSLVLHKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 240

Query: 273 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 332
           SSGILIMGRTSTSTSLLHSVFREKTF+ASKHANHVDSEKKILLKRYWALVIGSPRRSKGL
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 300

Query: 333 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 392
           ITAPLGKIVLDDGRSE+IMVIDNAEPMPFQQAIT+YKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLGKIVLDDGRSEQIMVIDNAEPMPFQQAITQYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 393 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 452
           RVHCAEVLGTPIVGDYKYGWQAHKNWKPF  NFHKNQGKKQFLPFGLDFESGSIAE NPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFCSNFHKNQGKKQFLPFGLDFESGSIAENNPR 420

Query: 453 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 504
           LHLHCKQLTLPDVSFALHNDSLD+DCSRLECLELDAPLPSYMQKSWDIINS
Sbjct: 421 LHLHCKQLTLPDVSFALHNDSLDRDCSRLECLELDAPLPSYMQKSWDIINS 471

BLAST of Pay0002705 vs. NCBI nr
Match: XP_038888757.1 (RNA pseudouridine synthase 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 850.9 bits (2197), Expect = 5.6e-243
Identity = 416/470 (88.51%), Postives = 431/470 (91.70%), Query Frame = 0

Query: 33  MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 92
           MVLP L R SVILR   S   P  E  +FSIFH LKSTLALSN +E VS +KNEKGSNRW
Sbjct: 1   MVLPALIRRSVILRIPLSSAAPVGESFHFSIFHELKSTLALSNTTETVSHRKNEKGSNRW 60

Query: 93  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 152
           FTLPPYTATVN S+LGNRIL RG QAE E STAAITALKWVI CCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNGSLLGNRILKRGDQAEAEISTAAITALKWVIRCCPELPRSLVQKLFRLR 120

Query: 153 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 212
           KVRRGSSMI NSE NLEARDQLKRVAAKDILN+GDQIFLPIS QVF TEK RDYCNK+EE
Sbjct: 121 KVRRGSSMIGNSECNLEARDQLKRVAAKDILNIGDQIFLPISVQVFPTEKARDYCNKEEE 180

Query: 213 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 272
            F+HSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSY YSEPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240

Query: 273 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 332
           SSGILIMGRTSTSTSLLHSVFREKTF+ASKHANHVDSEKKIL KRYWALVIGSPRRSKGL
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFEASKHANHVDSEKKILQKRYWALVIGSPRRSKGL 300

Query: 333 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 392
           ITAPLGK V+DDGRSE+IMVIDNAE MPFQQAITRYKVIKSHHGYTWLELCP+TGRKHQL
Sbjct: 301 ITAPLGKFVMDDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCPVTGRKHQL 360

Query: 393 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 452
           RVHCAEVLGTPIVGDYKYGWQAH+NWKPFH NFHKN+GKKQ  PFGLDFESGSIAE NPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSFPFGLDFESGSIAENNPR 420

Query: 453 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIIN 503
           LHLHCKQLTLPDVSFALHNDS+DQ CSRLECLELDAPLP YMQ+SWDIIN
Sbjct: 421 LHLHCKQLTLPDVSFALHNDSIDQKCSRLECLELDAPLPLYMQRSWDIIN 470

BLAST of Pay0002705 vs. NCBI nr
Match: XP_023553811.1 (RNA pseudouridine synthase 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 809.3 bits (2089), Expect = 1.9e-230
Identity = 396/471 (84.08%), Postives = 421/471 (89.38%), Query Frame = 0

Query: 33  MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 92
           MVLP + R SVI R+ PS   P  E L+FSIF   KST ALSNA+E VS+QKNEKGSNRW
Sbjct: 1   MVLPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSNATETVSEQKNEKGSNRW 60

Query: 93  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 152
           FTLPP+TATVN S LGNRI+N GAQAE ETST AITALKWV  CCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATVNGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 153 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 212
           KVRRGSSMIENSE NLEARDQLKRVAA DILN+GD++ LPIS QVF TE+  D CNK+E+
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 213 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 272
            F+HSLVLYKDPAIL INKPPGLPVQGGIGVKRSLDELAASCLSY YSEPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240

Query: 273 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 332
           SSGILIMGRTSTSTS+LHS+FREKT  ASKHANHVD EKKIL KRYWALVIGSPRR+KGL
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDREKKILQKRYWALVIGSPRRTKGL 300

Query: 333 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 392
           ITAPL K+VLDDGRSE+IMV+DNAE MPFQ AIT YKVIK+HHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLVKMVLDDGRSEQIMVVDNAESMPFQHAITTYKVIKTHHGYTWLELCPLTGRKHQL 360

Query: 393 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 452
           RVHCAEVLGTPIVGDYKYGWQAH+NWKPF +N HKNQGKKQ LPFGLDFESGSIAE NPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENNPR 420

Query: 453 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 504
           LHLHCK LTLPDVS ALHNDSLD DCSRLE LELDAPLPSYMQ+SWDIIN+
Sbjct: 421 LHLHCKHLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

BLAST of Pay0002705 vs. NCBI nr
Match: KAG7011636.1 (RNA pseudouridine synthase 4, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 806.6 bits (2082), Expect = 1.2e-229
Identity = 395/471 (83.86%), Postives = 421/471 (89.38%), Query Frame = 0

Query: 33  MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 92
           MV P + R SVI R+ PS   P  E L+FSIF   KST ALS+A+E VS+QKNEKGSNRW
Sbjct: 1   MVFPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSSATETVSEQKNEKGSNRW 60

Query: 93  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 152
           FTLPP+TAT N S LGNRI+N GAQAE ETST AITALKWV  CCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 153 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 212
           KVRRGSSMIENSE NLEARDQLKRVAA DILN+GD++ LPIS QVF TE+  D CNK+E+
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 213 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 272
            F+HSLVLYKDPAIL INKPPGLPVQGGIGVKRSLD+LAASCLSY YSEPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDRD 240

Query: 273 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 332
           SSGILIMGRTSTSTS+LHS+FREKT  ASKHANHVDSEKKIL KRYWALVIGSPRR+KGL
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDSEKKILQKRYWALVIGSPRRTKGL 300

Query: 333 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 392
           ITAPL K+VLDDGRSE+IMVIDNAE MPFQ AIT YKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLVKMVLDDGRSEQIMVIDNAESMPFQHAITTYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 393 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 452
           RVHCAEVLGTPIVGDYKYGWQAH+NWKPF +N HKNQGKKQ LPFGLDFESGSIAE +PR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENSPR 420

Query: 453 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 504
           LHLHCKQLTLPDVS ALHNDSLD DCSRLE LELDAPLPSYMQ+SWDIIN+
Sbjct: 421 LHLHCKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

BLAST of Pay0002705 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 449.5 bits (1155), Expect = 3.5e-126
Identity = 233/441 (52.83%), Postives = 303/441 (68.71%), Query Frame = 0

Query: 72  ALSNASEPVSQQKNEKGSNRWFTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALK 131
           A   ++   S  + +    +W TLPP++ T++ + +G  +L+ G   +  T  +  TAL+
Sbjct: 35  AAHQSTRSYSTTQTDDSRGKWLTLPPFSPTIDGTAVGKDLLSDGDSVKSSTDNSKTTALR 94

Query: 132 WVIHCCPELPRSLVQKLFRLRKVRRGSSM-IENSEYNLEARDQLKRVAAKDILNVGDQIF 191
           W++ C P+LPR+LVQKLFRLR+VRR  SM ++  E     R QLKRVAAK+ LNVGD+I+
Sbjct: 95  WILRCRPDLPRTLVQKLFRLRQVRREMSMSVDGDELQ---RSQLKRVAAKESLNVGDRIY 154

Query: 192 LPISA-----QVFSTEKTRDYCNKDEETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKR 251
           LP+S      Q    +K    C+ +E  F+ SLVLYKDPAI+V+NKP GL VQGG G+K 
Sbjct: 155 LPLSVDNDTPQTPPAKKESFQCSDEERKFVCSLVLYKDPAIIVLNKPHGLAVQGGSGIKT 214

Query: 252 SLDELAASCLSYGYSEPPRLVHRLDRDSSGILIMGRTSTSTSLLHSVFREKTFDASKHAN 311
           S+DELAASCL +  SE PRLVHRLDRD SG+L++ RT T+ ++LHS+FREKT  AS +  
Sbjct: 215 SIDELAASCLKFDKSESPRLVHRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYG- 274

Query: 312 HVDSEKKILLKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAI 371
            V    K L ++Y ALVIG P R +G I+APL K+V+DDG+SERI V DN E +  Q AI
Sbjct: 275 -VKKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAI 334

Query: 372 TRYKVIKSH-HGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYN 431
           T Y+VI+S  HGYTWLEL PLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHK  +PF  +
Sbjct: 335 TEYRVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKAREPF-VS 394

Query: 432 FHKNQGKKQFLPFGLDFESGSIAETNPRLHLHCKQLTLPDVSFALH--NDSLDQDCSRLE 491
              N  K+   PFGLD + G ++   P LHLH KQ+ LP++S  L     S D D S L+
Sbjct: 395 SENNPTKQSSSPFGLDLDGGDVSSKQPHLHLHSKQIDLPNISQLLEKMQVSSDSDISDLD 454

Query: 492 CLELDAPLPSYMQKSWDIINS 504
            L+ DAPLPS+MQ S++++ S
Sbjct: 455 SLKFDAPLPSHMQLSFNLLKS 469

BLAST of Pay0002705 vs. TAIR 10
Match: AT1G78910.1 (Pseudouridine synthase family protein )

HSP 1 Score: 300.1 bits (767), Expect = 3.4e-81
Identity = 167/419 (39.86%), Postives = 246/419 (58.71%), Query Frame = 0

Query: 113 NRGAQAEVETSTAAITALKWVIHCCPEL---------PRSLVQKLFRLRKVRRGSSMIEN 172
           N GA  +   S   +TA+ WV +   E+          + LVQ  FR R+        + 
Sbjct: 67  NAGADGDDGDSGGHVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRR--------DA 126

Query: 173 SEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRD-------YCNKDEETFMH 232
           S    +    ++++   +++ +GD+I+LP+S       K  D       Y N DE  ++ 
Sbjct: 127 SREKEDGAIPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQ 186

Query: 233 SLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRDSSGI 292
            LV +KD AI+V+NKPP LPV+G + +  S+D LAA+ LS+G  E PRLVHRLDR++SG+
Sbjct: 187 RLVRFKDSAIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGL 246

Query: 293 LIMGRTSTSTSLLHSVFRE-KTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGLITA 352
           L+MGRT  S   LHSVF + K  ++S  A +   E   + ++YWALVIGSP+  +GLI+A
Sbjct: 247 LVMGRTKESIDYLHSVFSDYKGRNSSCKAWNKACE--AMYQQYWALVIGSPKEKEGLISA 306

Query: 353 PLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVI-KSHHGYTWLELCPLTGRKHQLRV 412
           PL K++LDDG+++R+++   +     Q AIT YKV+    +G +W+EL P+T RKHQLRV
Sbjct: 307 PLSKVLLDDGKTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRV 366

Query: 413 HCAEVLGTPIVGDYKYGWQAHKNWKPF-HYNFHKNQGKKQFL--PFGLDFESGSIAETNP 472
           HCAE LGTPIVGDYKYGW  HK WK     +     GK   L  P GLD + GS+    P
Sbjct: 367 HCAEALGTPIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVP 426

Query: 473 RLHLHCKQLTLPDVSFALHNDSLDQD-------CSRLECLELDAPLPSYMQKSWDIINS 504
            LHLHC+++ LP+++  LH  +  +          + + L   A +PS+M+ SW++++S
Sbjct: 427 LLHLHCREMVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSS 475

BLAST of Pay0002705 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 79.0 bits (193), Expect = 1.2e-14
Identity = 98/405 (24.20%), Postives = 165/405 (40.74%), Query Frame = 0

Query: 41  PSVILRTSPSYTDPSSE----ILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRWFTLP 100
           PS  L T+ S   PSS      ++FS F  + + +  S+                   +P
Sbjct: 8   PSFSLTTAKSLRYPSSPSSSLSIFFSFFPKVSNFVRASSG------------------IP 67

Query: 101 PYTATVNASILGNRILNRGAQAE--VETSTAAITALKWVIHCCPELPRSLVQKLFRLRKV 160
              A     I+  R+ N G + E  V+ +   I    W+      + R+ VQ   RL  V
Sbjct: 68  NLVACSPTEIIIPRVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLV 127

Query: 161 RRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEETF 220
                +++   +N+++ D++    ++           P+ A+  + +   D   +D+   
Sbjct: 128 TVNGRVVDKVSHNVKSGDEVNCTISE---------LQPLKAE--AEDIPLDIVYEDK--- 187

Query: 221 MHSLVLYKDPAILVINKPPGLP---VQGGIGVKRSL----------------------DE 280
            H LV+ K PA +V++  PG P   +  GI    SL                      +E
Sbjct: 188 -HVLVVNK-PAHMVVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETFSDDEE 247

Query: 281 LAASCLSYGYSEPPRLVHRLDRDSSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDS 340
           +  S  SY  S  P +VHRLD+ ++G+L++ +   S + L   F+  T +          
Sbjct: 248 MTTSPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIE---------- 307

Query: 341 EKKILLKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSERIMVI-DNAEPMPFQQAITRY 400
                 + Y +L  G P   +G I  P+G+   D     R+  I         + A +RY
Sbjct: 308 ------RVYVSLTTGVPSPPQGRIEIPIGR---DSSNRIRMAAIPGGVRGGRARHAASRY 358

Query: 401 KVIKSH--HGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYG 412
           KVI++    G   +E    TGR HQ+R H A+ +G P++GD  YG
Sbjct: 368 KVIETFAGGGSALVEWRLETGRTHQIRAH-AKYMGVPLLGDEVYG 358

BLAST of Pay0002705 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 69.3 bits (168), Expect = 9.9e-12
Identity = 63/211 (29.86%), Postives = 100/211 (47.39%), Query Frame = 0

Query: 209 KDEETFMHSLVLYKDPAILVINKPPGLPV-QGGIGVKRSLDELAASCL----SY-GYSEP 268
           K+ +T     VLY+D  ++ +NKP GL V  GG+  +R++      C     SY G  E 
Sbjct: 89  KEPDTPYSLEVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRES 148

Query: 269 PR--LVHRLDRDSSGILIMGRTSTSTSLLHSVFREKT-FDASKHANHVDSEKKILLKRYW 328
           P    VHRL R +SGIL+  +T  + + L + F E T    S + +      + L K Y 
Sbjct: 149 PHPVPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYR 208

Query: 329 ALVIGSPRRSKGLITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTW 388
           AL  G     + +I  P+G +V   G ++ + V        F +     +  + +     
Sbjct: 209 ALADGIVEEDEVVIKQPIG-VVRYPGVAQGLYVASPEGKPAFSKVFVLERDREKNCSLVK 268

Query: 389 LELCPLTGRKHQLRVHCAEVLGTPIVGDYKY 411
           +E+   +GR HQ+R+H A  +G P+VGD  Y
Sbjct: 269 VEI--QSGRPHQIRIHLA-YMGHPLVGDPLY 295

BLAST of Pay0002705 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 64.3 bits (155), Expect = 3.2e-10
Identity = 60/206 (29.13%), Postives = 97/206 (47.09%), Query Frame = 0

Query: 209 KDEETFMHSLVLYKDPAILVINKPPGLPV-QGGIGVKRSLDELAASCL----SY-GYSEP 268
           K+ +T     VLY+D  ++ +NKP GL V  GG+  +R++      C     SY G  E 
Sbjct: 119 KEPDTPYSLEVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRES 178

Query: 269 PR--LVHRLDRDSSGILIMGRTSTSTSLLHSVFREKT-FDASKHANHVDSEKKILLKRYW 328
           P    VHRL R +SGIL+  +T  + + L + F E T    S + +      + L K Y 
Sbjct: 179 PHPVPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYR 238

Query: 329 ALVIGSPRRSKGLITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTW 388
           AL  G     + +I  P+G +V   G ++ + V        F +     +  + +     
Sbjct: 239 ALADGIVEEDEVVIKQPIG-VVRYPGVAQGLYVASPEGKPAFSKVFVLERDREKNCSLVK 298

Query: 389 LELCPLTGRKHQLRVHCAEVLGTPIV 406
           +E+   +GR HQ+R+H A  +G P+V
Sbjct: 299 VEI--QSGRPHQIRIHLA-YMGHPLV 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LT724.9e-12552.83RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q0J4D43.7e-8040.51RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=3... [more]
Q5XET64.8e-8039.86RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q69K077.2e-7649.68RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=3... [more]
Q68XB24.3e-2831.56Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATC... [more]
Match NameE-valueIdentityDescription
A0A5D3C4F52.2e-277100.00RNA pseudouridine synthase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3BZE72.2e-277100.00RNA pseudouridine synthase 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495... [more]
A0A6J1GMP97.7e-23084.08RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1D4326.7e-22681.99RNA pseudouridine synthase 4, mitochondrial OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1IBC52.0e-22582.84RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_008454996.14.6e-277100.00PREDICTED: RNA pseudouridine synthase 4, mitochondrial [Cucumis melo] >KAA003133... [more]
XP_011658889.11.6e-26194.90RNA pseudouridine synthase 4, mitochondrial [Cucumis sativus] >KGN43951.2 hypoth... [more]
XP_038888757.15.6e-24388.51RNA pseudouridine synthase 4, mitochondrial [Benincasa hispida][more]
XP_023553811.11.9e-23084.08RNA pseudouridine synthase 4, mitochondrial [Cucurbita pepo subsp. pepo][more]
KAG7011636.11.2e-22983.86RNA pseudouridine synthase 4, mitochondrial, partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
AT3G19440.13.5e-12652.83Pseudouridine synthase family protein [more]
AT1G78910.13.4e-8139.86Pseudouridine synthase family protein [more]
AT1G76050.21.2e-1424.20Pseudouridine synthase family protein [more]
AT3G52260.29.9e-1229.86Pseudouridine synthase family protein [more]
AT3G52260.33.2e-1029.13Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 208..470
e-value: 1.5E-52
score: 180.3
NoneNo IPR availablePANTHERPTHR21600:SF53RNA PSEUDOURIDINE SYNTHASE 3, MITOCHONDRIALcoord: 79..501
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 79..501
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 226..397
e-value: 2.6E-24
score: 86.1
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 226..462
e-value: 1.08361E-53
score: 177.144
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 212..499

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0002705.1Pay0002705.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000455 enzyme-directed rRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding