Pay0001727 (gene) Melon (Payzawat) v1

Overview
NamePay0001727
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
DescriptionAuxin-responsive GH3 family protein
LocationContig00140_ERROPOS1134973: 156465 .. 158607 (-)
RNA-Seq ExpressionPay0001727
SyntenyPay0001727
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCAACTTACAACCAAATATTTACTTTTCTTCTTAATAATGGCTGCTAGTTCTTCGGTTTCCCATGCCAAGGCTCTCCAATTCATTGAGGAGATTACTACAAATGCTGACTCGGTTCAACAGAAGGTGTTGGCTGAGATTTTGAATAGGAATTCTGATACCGAGTATTTGAAACGGTTTCGACTGAGTAGAAGTGCAGGTAGAGATGAATTTAAGTCAAATGTTCGGGTGGTTACTTACGAAGACCTTCAACCCGATATCCAACGTATTGCTAATGGCGATCGCTCCCCTATTTTTTCCTCTCATCCTATTTCCGAGTTTCTTACCAGGTGAATAATTATGAAAATACAATCAATTATGCAAAAATTTTATATTTTTCCAAGTTCATTTTTTTTTTCTGAATTGCGTTCGATCTGATTTTTGAAATTCAGTTCTGGGACATCTGCTGGGGAGAGGAAACTAATGCCGACAATTAAAGGAGAAATGGAACGTCGTCAGCTACTTTACAGCCTTCTCATGCCCATCATGAACAAGTAATTTAACTGAATTCAATTCATGAAGAATTATATATATTAAGATTAGCTCCTCATTTTGATTTTTTTTTTTTAAATTAACAAATAAATAATAAATTTTCAGGTATGTACCGGGGTTAGACAAAGGGAAAGGGCTTTACTTTTTATTCGTGAAGGCAGAGACCAAGACCCCTGGAGGTTTACCGGCACGTCCGGTACTTACTAGCTATTACAAGAGCGACATCTTCATGACCAGACCCTATGATCCTTTCAATGACTACACTAGCCCAAACGAGGCCATTCTATGTGCCAATTCCTTCCAAAGCATGTACACTCAAATGTTATGTGGTTTATTGATGCGTGACCAAGTCCTCCGTGTTGGCGCCGTCTTTGCCTCCGGTCTCCTCCGTGCCATTCACTTCCTTCAGCTAAACTGGAAACAGCTGGCACACGACATCTCCACGGCAACCTTAAATCCCAAAATCACGGACTCTTGTCTTCGTGAATGTATAGTATCCAAATATTTAACAAACCCGAATTCGGAGCTGGCGGAACTCATCTCAAAGGAATGCTCGACGGAGGAATGGGAAGGGATTATCACGAGAATATGGCCGAATACTAAATATTTGGATGTGATTGTGACTGGAGCCATGGCTCAGTATATTCCCACCCTCGAATTTTACAGCGGTGGTTTACCCATGGCTTGCACTATGTATGCTTCTTCCGAGTGTTATTTTGGGGTAAATTTAAACCCAATGTGTAAACCCTCGGATGTGACTTACACCATCATGCCAAACATGTGCTACTTCGAATTCATCCCCCTCGACAACGACATGTCGTCTCCTCCAACTCAACTTGTTGACCTCGCAGACGTGGAAGTCGGGAAAGAGTACGAACTCGTGATCACAACATACTCTGGACTCTGTCGTTACCGAGTCGGTGACATCCTACACGTCACTGGGTTCCACAACGCGGCCCCACAGTTTCGATTCGTGAGGAGGAAGAATGTTCTTTTGAGCATTGATTCCGACAAGACGGATGAAGCCGAGTTACAAAAGGCCATAGACAACGCCGCGCTGTTGTTGCGGGGGTTCAACACTAGCGTGGTGGAGTATACGAGCTATGCGGATACGAAAACAATCCCAGGACATTACGTCATATATTGGGAGCTTCTTGTGAAAGACGAAGAAACTGGAAATTTCCCTCCAGGAGAGATTTTAAATCAGAGTTGCTTGGCTATTGAAGAGAGTTTGAACTCGGTTTACCGACAAGGCCGAGTTGCGGATAACTCAATTGGACCGTTGGAGATTAGGGTGGTAAGAAAGGGGACGTTTGAAGAGTTAATGGACTATGCGATCTCGAGGGGTGCGTCGATCAACCAGTACAAGGCGCCACGATGCGTAAACTTCACGCCCATTATCGAGCTTCTCGACTCTAGGGTGACTTCTGTTCACTTTAGTCCATCCAAGCCACACTGGACACCAGAACCACGCGGCTAGACATTAATCAGTTTTTCAGGGTTGCCTTCTTAAAATCGTTTTGATACATTTTATTTTATAATATGTATGCTTCTTATTCCTTCATAATTAATGGGTACAAAGAATAAGTATTTTCACATTTTCTCACCCCAC

mRNA sequence

CTCCAACTTACAACCAAATATTTACTTTTCTTCTTAATAATGGCTGCTAGTTCTTCGGTTTCCCATGCCAAGGCTCTCCAATTCATTGAGGAGATTACTACAAATGCTGACTCGGTTCAACAGAAGGTGTTGGCTGAGATTTTGAATAGGAATTCTGATACCGAGTATTTGAAACGGTTTCGACTGAGTAGAAGTGCAGGTAGAGATGAATTTAAGTCAAATGTTCGGGTGGTTACTTACGAAGACCTTCAACCCGATATCCAACGTATTGCTAATGGCGATCGCTCCCCTATTTTTTCCTCTCATCCTATTTCCGAGTTTCTTACCAGTTCTGGGACATCTGCTGGGGAGAGGAAACTAATGCCGACAATTAAAGGAGAAATGGAACGTCGTCAGCTACTTTACAGCCTTCTCATGCCCATCATGAACAAGTATGTACCGGGGTTAGACAAAGGGAAAGGGCTTTACTTTTTATTCGTGAAGGCAGAGACCAAGACCCCTGGAGGTTTACCGGCACGTCCGGTACTTACTAGCTATTACAAGAGCGACATCTTCATGACCAGACCCTATGATCCTTTCAATGACTACACTAGCCCAAACGAGGCCATTCTATGTGCCAATTCCTTCCAAAGCATGTACACTCAAATGTTATGTGGTTTATTGATGCGTGACCAAGTCCTCCGTGTTGGCGCCGTCTTTGCCTCCGGTCTCCTCCGTGCCATTCACTTCCTTCAGCTAAACTGGAAACAGCTGGCACACGACATCTCCACGGCAACCTTAAATCCCAAAATCACGGACTCTTGTCTTCGTGAATGTATAGTATCCAAATATTTAACAAACCCGAATTCGGAGCTGGCGGAACTCATCTCAAAGGAATGCTCGACGGAGGAATGGGAAGGGATTATCACGAGAATATGGCCGAATACTAAATATTTGGATGTGATTGTGACTGGAGCCATGGCTCAGTATATTCCCACCCTCGAATTTTACAGCGGTGGTTTACCCATGGCTTGCACTATGTATGCTTCTTCCGAGTGTTATTTTGGGGTAAATTTAAACCCAATGTGTAAACCCTCGGATGTGACTTACACCATCATGCCAAACATGTGCTACTTCGAATTCATCCCCCTCGACAACGACATGTCGTCTCCTCCAACTCAACTTGTTGACCTCGCAGACGTGGAAGTCGGGAAAGAGTACGAACTCGTGATCACAACATACTCTGGACTCTGTCGTTACCGAGTCGGTGACATCCTACACGTCACTGGGTTCCACAACGCGGCCCCACAGTTTCGATTCGTGAGGAGGAAGAATGTTCTTTTGAGCATTGATTCCGACAAGACGGATGAAGCCGAGTTACAAAAGGCCATAGACAACGCCGCGCTGTTGTTGCGGGGGTTCAACACTAGCGTGGTGGAGTATACGAGCTATGCGGATACGAAAACAATCCCAGGACATTACGTCATATATTGGGAGCTTCTTGTGAAAGACGAAGAAACTGGAAATTTCCCTCCAGGAGAGATTTTAAATCAGAGTTGCTTGGCTATTGAAGAGAGTTTGAACTCGGTTTACCGACAAGGCCGAGTTGCGGATAACTCAATTGGACCGTTGGAGATTAGGGTGGTAAGAAAGGGGACGTTTGAAGAGTTAATGGACTATGCGATCTCGAGGGGTGCGTCGATCAACCAGTACAAGGCGCCACGATGCGTAAACTTCACGCCCATTATCGAGCTTCTCGACTCTAGGGTGACTTCTGTTCACTTTAGTCCATCCAAGCCACACTGGACACCAGAACCACGCGGCTAGACATTAATCAGTTTTTCAGGGTTGCCTTCTTAAAATCGTTTTGATACATTTTATTTTATAATATGTATGCTTCTTATTCCTTCATAATTAATGGGTACAAAGAATAAGTATTTTCACATTTTCTCACCCCAC

Coding sequence (CDS)

ATGGCTGCTAGTTCTTCGGTTTCCCATGCCAAGGCTCTCCAATTCATTGAGGAGATTACTACAAATGCTGACTCGGTTCAACAGAAGGTGTTGGCTGAGATTTTGAATAGGAATTCTGATACCGAGTATTTGAAACGGTTTCGACTGAGTAGAAGTGCAGGTAGAGATGAATTTAAGTCAAATGTTCGGGTGGTTACTTACGAAGACCTTCAACCCGATATCCAACGTATTGCTAATGGCGATCGCTCCCCTATTTTTTCCTCTCATCCTATTTCCGAGTTTCTTACCAGTTCTGGGACATCTGCTGGGGAGAGGAAACTAATGCCGACAATTAAAGGAGAAATGGAACGTCGTCAGCTACTTTACAGCCTTCTCATGCCCATCATGAACAAGTATGTACCGGGGTTAGACAAAGGGAAAGGGCTTTACTTTTTATTCGTGAAGGCAGAGACCAAGACCCCTGGAGGTTTACCGGCACGTCCGGTACTTACTAGCTATTACAAGAGCGACATCTTCATGACCAGACCCTATGATCCTTTCAATGACTACACTAGCCCAAACGAGGCCATTCTATGTGCCAATTCCTTCCAAAGCATGTACACTCAAATGTTATGTGGTTTATTGATGCGTGACCAAGTCCTCCGTGTTGGCGCCGTCTTTGCCTCCGGTCTCCTCCGTGCCATTCACTTCCTTCAGCTAAACTGGAAACAGCTGGCACACGACATCTCCACGGCAACCTTAAATCCCAAAATCACGGACTCTTGTCTTCGTGAATGTATAGTATCCAAATATTTAACAAACCCGAATTCGGAGCTGGCGGAACTCATCTCAAAGGAATGCTCGACGGAGGAATGGGAAGGGATTATCACGAGAATATGGCCGAATACTAAATATTTGGATGTGATTGTGACTGGAGCCATGGCTCAGTATATTCCCACCCTCGAATTTTACAGCGGTGGTTTACCCATGGCTTGCACTATGTATGCTTCTTCCGAGTGTTATTTTGGGGTAAATTTAAACCCAATGTGTAAACCCTCGGATGTGACTTACACCATCATGCCAAACATGTGCTACTTCGAATTCATCCCCCTCGACAACGACATGTCGTCTCCTCCAACTCAACTTGTTGACCTCGCAGACGTGGAAGTCGGGAAAGAGTACGAACTCGTGATCACAACATACTCTGGACTCTGTCGTTACCGAGTCGGTGACATCCTACACGTCACTGGGTTCCACAACGCGGCCCCACAGTTTCGATTCGTGAGGAGGAAGAATGTTCTTTTGAGCATTGATTCCGACAAGACGGATGAAGCCGAGTTACAAAAGGCCATAGACAACGCCGCGCTGTTGTTGCGGGGGTTCAACACTAGCGTGGTGGAGTATACGAGCTATGCGGATACGAAAACAATCCCAGGACATTACGTCATATATTGGGAGCTTCTTGTGAAAGACGAAGAAACTGGAAATTTCCCTCCAGGAGAGATTTTAAATCAGAGTTGCTTGGCTATTGAAGAGAGTTTGAACTCGGTTTACCGACAAGGCCGAGTTGCGGATAACTCAATTGGACCGTTGGAGATTAGGGTGGTAAGAAAGGGGACGTTTGAAGAGTTAATGGACTATGCGATCTCGAGGGGTGCGTCGATCAACCAGTACAAGGCGCCACGATGCGTAAACTTCACGCCCATTATCGAGCTTCTCGACTCTAGGGTGACTTCTGTTCACTTTAGTCCATCCAAGCCACACTGGACACCAGAACCACGCGGCTAG

Protein sequence

MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKSNVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQLLYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPFNDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAHDISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFEFIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWELLVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDYAISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG
Homology
BLAST of Pay0001727 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 1.2e-255
Identity = 435/579 (75.13%), Postives = 492/579 (84.97%), Query Frame = 0

Query: 10  AKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKSNVRVVTYED 69
           AKAL+FIEE+T NAD+VQ+ +LAEIL RN+DTEYL+RF L  +  RD FK+ + V+TYED
Sbjct: 20  AKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVITYED 79

Query: 70  LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQLLYSLLMPIM 129
           LQP+IQRIA+GDRSPI S+HPISEFLTSSGTSAGERKLMPTIK E++RRQLLYSLLMP+M
Sbjct: 80  LQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLMPVM 139

Query: 130 NKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPFNDYTSPNEA 189
           N YVPGLDKGKG+YFLFVK+ETKTPGGLPARPVLTSYYKS+ F +RPYDP+N YTSPNEA
Sbjct: 140 NLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSPNEA 199

Query: 190 ILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAHDISTATLNP 249
           ILC +SFQSMYTQMLCGLL R  VLRVGAVFASGLLRAI FLQL+W + AHDI    L+ 
Sbjct: 200 ILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGCLDS 259

Query: 250 KITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQ 309
           +ITD  +R+C+    +  P+  LAE I +EC ++ WE IITRIWPNTKYLDVIVTGAMAQ
Sbjct: 260 EITDPSIRQCMSG--ILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMAQ 319

Query: 310 YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFEFIPLDNDMS 369
           YIPTLE+YSGGLPMACTMYASSECYFG+NLNPM KPS+V+YTIMPNM YFEFIPL     
Sbjct: 320 YIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGG--- 379

Query: 370 SPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFVRRKNVLLS 429
              T+ V+L DV +GKEYELV+TTY+GLCRYRVGDIL VTGFHN+APQF FVRRKNVLLS
Sbjct: 380 ---TKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNVLLS 439

Query: 430 IDSDKTDEAELQKAIDNAALLL-RGFNTSVVEYTSYADTKTIPGHYVIYWELLVKDEETG 489
           IDSDKTDE+ELQKA++NA+ +L     + V EYTSYADT TIPGHYV+YWELLV+D    
Sbjct: 440 IDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRD--GA 499

Query: 490 NFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDYAISRGASI 549
             P  E L + CL +EESLNSVYRQ RVADNS+GPLEIRVVR GTFEELMDYAISRGASI
Sbjct: 500 RQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGASI 559

Query: 550 NQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPR 588
           NQYK PRCVNFTPI+ELLDSRV S HFSPS PHWTPE R
Sbjct: 560 NQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERR 588

BLAST of Pay0001727 vs. ExPASy Swiss-Prot
Match: O22190 (Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=GH3.3 PE=1 SV=1)

HSP 1 Score: 865.9 bits (2236), Expect = 2.6e-250
Identity = 434/588 (73.81%), Postives = 498/588 (84.69%), Query Frame = 0

Query: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS 60
           M  S S    KAL+FIEE+T N D VQ+KV+ EIL+RNSDTEYLKRF L     R  FK+
Sbjct: 11  MMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKT 70

Query: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL 120
            V VV Y+DL+P+IQRIANGDRS I SS+PI+EFLTSSGTSAGERKLMPTI  +M+RRQL
Sbjct: 71  KVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQL 130

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180
           LYSLLMP+MN YVPGLDKGK LYFLFVK E+KTPGGLPARPVLTSYYKS+ F  RP DP+
Sbjct: 131 LYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPY 190

Query: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240
           N YTSPNEAILC +S QSMYTQMLCGLLMR +VLR+GAVFASGLLRAI FLQ NWK+LA 
Sbjct: 191 NVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELAD 250

Query: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTE-EWEGIITRIWPNTKYL 300
           DIST TL+ +I+D  ++E  +SK LT P+ ELA+ I+  C  +  WEGIIT+IWPNTKYL
Sbjct: 251 DISTGTLSSRISDPAIKES-MSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYL 310

Query: 301 DVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYF 360
           DVIVTGAMAQYIP LE+YSGGLPMACTMYASSE YFG+NL PMCKPS+V+YTIMPNM YF
Sbjct: 311 DVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYF 370

Query: 361 EFIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFR 420
           EF+P  +++ +  ++LV+LADVEVGKEYELVITTY+GL RYRVGDIL VTGF+N+APQF+
Sbjct: 371 EFLP-HHEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFK 430

Query: 421 FVRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWE 480
           FVRRKNVLLSI+SDKTDEAELQ A++NA+LLL    T V+EYTSYA+TKTIPGHYVIYWE
Sbjct: 431 FVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWE 490

Query: 481 LLVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMD 540
           LLVKD+   N P  E++ + CL +EESLNSVYRQ RVAD SIGPLEIRVV+ GTFEELMD
Sbjct: 491 LLVKDQT--NPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMD 550

Query: 541 YAISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPR 588
           YAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSP+ PHW+PE R
Sbjct: 551 YAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERR 594

BLAST of Pay0001727 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 846.7 bits (2186), Expect = 1.6e-244
Identity = 420/577 (72.79%), Postives = 478/577 (82.84%), Query Frame = 0

Query: 13  LQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKSNVRVVTYEDLQP 72
           L+FI+E+TTN D+VQ++VL EIL RN+ TEYL +  L  +  R  F++ V VV+Y+DLQP
Sbjct: 30  LRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQP 89

Query: 73  DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQLLYSLLMPIMNKY 132
            IQRIANGDRSPI S+HP+SEFLTSSGTSAGERKLMPTI  E++RRQLLYSLLMP+MN Y
Sbjct: 90  YIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLY 149

Query: 133 VPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPFNDYTSPNEAILC 192
           VPGLDKGKGLYFLFVK+ETKTPGGL ARPVLTSYYKSD F  RPYDP+++YTSP  AILC
Sbjct: 150 VPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILC 209

Query: 193 ANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAHDISTATLNPKIT 252
           A++FQSMY QM+CGL  R+ VLR+GAVFASGLLRAI FLQLNW+QLA DI +  L P++T
Sbjct: 210 ADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVT 269

Query: 253 DSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQYIP 312
           D  +RE + +  L  P+ ELA+LI  ECS  +W GIITR+WPNTKYLDVIVTGAMAQYIP
Sbjct: 270 DPSVREAVAAILL--PDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIP 329

Query: 313 TLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFEFIPLDND--MSS 372
           TLEFYSGGLPMACTMYASSECYFG+NL PMC PS+V+YTIMPNM YFEF+P+D     S 
Sbjct: 330 TLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASG 389

Query: 373 PPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFVRRKNVLLSI 432
             TQLVDLA VEVG+EYELVITTY+GL RYRVGD+L VTGFHNAAPQFRFVRRKNVLLSI
Sbjct: 390 DATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSI 449

Query: 433 DSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWELLVKDEETGNF 492
           +SDKTDEAELQ+A++ A+ LLR    SVVEYTS A TK IPGHYVIYWELL K       
Sbjct: 450 ESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKG-AGATV 509

Query: 493 PPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDYAISRGASINQ 552
              + L + CL +EE+LN+VYRQ RVAD SIGPLEIRVVR GTFEELMDYAISRGASINQ
Sbjct: 510 VDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQ 569

Query: 553 YKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPR 588
           YK PRCV F PI+ELLDSRV S HFSP+ PHWTP  R
Sbjct: 570 YKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

BLAST of Pay0001727 vs. ExPASy Swiss-Prot
Match: Q0D4Z6 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=1 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 1.6e-244
Identity = 420/577 (72.79%), Postives = 478/577 (82.84%), Query Frame = 0

Query: 13  LQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKSNVRVVTYEDLQP 72
           L+FI+E+TTN D+VQ++VL EIL RN+ TEYL +  L  +  R  F++ V VV+Y+DLQP
Sbjct: 30  LRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQP 89

Query: 73  DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQLLYSLLMPIMNKY 132
            IQRIANGDRSPI S+HP+SEFLTSSGTSAGERKLMPTI  E++RRQLLYSLLMP+MN Y
Sbjct: 90  YIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLY 149

Query: 133 VPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPFNDYTSPNEAILC 192
           VPGLDKGKGLYFLFVK+ETKTPGGL ARPVLTSYYKSD F  RPYDP+++YTSP  AILC
Sbjct: 150 VPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILC 209

Query: 193 ANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAHDISTATLNPKIT 252
           A++FQSMY QM+CGL  R+ VLR+GAVFASGLLRAI FLQLNW+QLA DI +  L P++T
Sbjct: 210 ADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVT 269

Query: 253 DSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQYIP 312
           D  +RE + +  L  P+ ELA+LI  ECS  +W GIITR+WPNTKYLDVIVTGAMAQYIP
Sbjct: 270 DPSVREAVAAILL--PDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIP 329

Query: 313 TLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFEFIPLDND--MSS 372
           TLEFYSGGLPMACTMYASSECYFG+NL PMC PS+V+YTIMPNM YFEF+P+D     S 
Sbjct: 330 TLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASG 389

Query: 373 PPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFVRRKNVLLSI 432
             TQLVDLA VEVG+EYELVITTY+GL RYRVGD+L VTGFHNAAPQFRFVRRKNVLLSI
Sbjct: 390 DATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSI 449

Query: 433 DSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWELLVKDEETGNF 492
           +SDKTDEAELQ+A++ A+ LLR    SVVEYTS A TK IPGHYVIYWELL K       
Sbjct: 450 ESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKG-AGATV 509

Query: 493 PPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDYAISRGASINQ 552
              + L + CL +EE+LN+VYRQ RVAD SIGPLEIRVVR GTFEELMDYAISRGASINQ
Sbjct: 510 VDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQ 569

Query: 553 YKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPR 588
           YK PRCV F PI+ELLDSRV S HFSP+ PHWTP  R
Sbjct: 570 YKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

BLAST of Pay0001727 vs. ExPASy Swiss-Prot
Match: P0C0M2 (Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.2 PE=2 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 1.2e-239
Identity = 412/597 (69.01%), Postives = 490/597 (82.08%), Query Frame = 0

Query: 2   AASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKSN 61
           A ++    A+ L+FIEE+T   D+VQ++VLA IL RN+  EYL+R  +     R+ FK+ 
Sbjct: 22  AVAACERDAEKLEFIEEMTRGFDAVQERVLAAILARNNGAEYLRRHGMEGRTDREAFKAR 81

Query: 62  VRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQLL 121
           V VVTYEDL+P+I+RIANGDRS I SSHPI+EFLTSSGTSAGERKLMPTI+ E++RRQ+L
Sbjct: 82  VPVVTYEDLRPEIERIANGDRSNIISSHPITEFLTSSGTSAGERKLMPTIEDELDRRQML 141

Query: 122 YSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPFN 181
           YSLLMP+MN YVPGLDKGKGLYFLF+K+ETKTPGGLPARPVLTSYYKSD F  RP+DP+N
Sbjct: 142 YSLLMPVMNLYVPGLDKGKGLYFLFIKSETKTPGGLPARPVLTSYYKSDHFKHRPFDPYN 201

Query: 182 DYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAHD 241
            YTSP  AILC ++FQSMY QMLCGL+ R +VLRVGAVFASGLLRAI FLQL+W++LAHD
Sbjct: 202 VYTSPTAAILCTDAFQSMYAQMLCGLVARAEVLRVGAVFASGLLRAIRFLQLHWRELAHD 261

Query: 242 ISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLDV 301
           I T TL+ K+T+  +R+  V++ L  P++ELA  +  EC  ++WEGIITR+WPNTKYLDV
Sbjct: 262 IRTGTLSAKVTEPSIRDA-VAEVLAAPDAELAAFVEAECGKDKWEGIITRMWPNTKYLDV 321

Query: 302 IVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFEF 361
           IVTGAMAQYIPTL+FYSGGLPMACTMYASSECYFG+NL PMC PS+V+YTIMPNM YFE 
Sbjct: 322 IVTGAMAQYIPTLKFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEL 381

Query: 362 IPLDND-----MSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAP 421
           +P D D       +PP +LVDLAD EVG+EYELVITTY+GLCRYRVGDIL VTGFHNAAP
Sbjct: 382 MPHDPDAPPLPRDAPPPRLVDLADAEVGREYELVITTYAGLCRYRVGDILQVTGFHNAAP 441

Query: 422 QFRFVRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVI 481
           QFRFVRRKNVLLSIDSDKTDEAELQ A++ A+ LL  +  S+VEYTS AD  TIPGHYV+
Sbjct: 442 QFRFVRRKNVLLSIDSDKTDEAELQAAVERASALLSPYGASIVEYTSQADATTIPGHYVV 501

Query: 482 YWELLVKDEETGNFPPGE------ILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVR 541
           YWEL+V+  E G +PP        +  + CL +EE+LN+VYRQGR  + +IGPLEIRVVR
Sbjct: 502 YWELMVR--EGGAWPPPAEEEGRGVFERCCLEMEEALNAVYRQGRNGE-AIGPLEIRVVR 561

Query: 542 KGTFEELMDYAISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPR 588
            GTFEE+MDYAISRGASINQYKAPRCV+F PIIELL+SRV S HFSP+ P ++P  +
Sbjct: 562 AGTFEEVMDYAISRGASINQYKAPRCVSFGPIIELLNSRVISKHFSPACPKYSPHKK 614

BLAST of Pay0001727 vs. ExPASy TrEMBL
Match: A0A1S3B1M0 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=LOC103484815 PE=3 SV=1)

HSP 1 Score: 1189.9 bits (3077), Expect = 0.0e+00
Identity = 588/588 (100.00%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS 60
           MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS
Sbjct: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS 60

Query: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL 120
           NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL
Sbjct: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL 120

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180
           LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF
Sbjct: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180

Query: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240
           NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH
Sbjct: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240

Query: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300
           DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD
Sbjct: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360
           VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE
Sbjct: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360

Query: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420
           FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF
Sbjct: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420

Query: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480
           VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL
Sbjct: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480

Query: 481 LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY 540
           LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY
Sbjct: 481 LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY 540

Query: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG 589
           AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG
Sbjct: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG 588

BLAST of Pay0001727 vs. ExPASy TrEMBL
Match: A0A5D3CR82 (Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004410 PE=3 SV=1)

HSP 1 Score: 1185.6 bits (3066), Expect = 0.0e+00
Identity = 586/588 (99.66%), Postives = 587/588 (99.83%), Query Frame = 0

Query: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS 60
           MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSR+AGRDEFKS
Sbjct: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRAAGRDEFKS 60

Query: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL 120
           NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIK EMERRQL
Sbjct: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180
           LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF
Sbjct: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180

Query: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240
           NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH
Sbjct: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240

Query: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300
           DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD
Sbjct: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360
           VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE
Sbjct: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360

Query: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420
           FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF
Sbjct: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420

Query: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480
           VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL
Sbjct: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480

Query: 481 LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY 540
           LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY
Sbjct: 481 LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY 540

Query: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG 589
           AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG
Sbjct: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG 588

BLAST of Pay0001727 vs. ExPASy TrEMBL
Match: A0A0A0KLF8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G492310 PE=3 SV=1)

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 571/588 (97.11%), Postives = 574/588 (97.62%), Query Frame = 0

Query: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS 60
           MA SSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRF+LSR+A RDEFKS
Sbjct: 1   MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFQLSRTASRDEFKS 60

Query: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL 120
           NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIK EMERRQL
Sbjct: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180
           LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKT GGL ARPVLTSYYKSDIF TRPYDPF
Sbjct: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTTGGLLARPVLTSYYKSDIFKTRPYDPF 180

Query: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240
           NDYTSPNEAILCANSFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAIHFLQ NWKQLAH
Sbjct: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH 240

Query: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300
           DISTATLNPKITD CLRECIVSKYLTNPN ELAELISKECSTEEWEGIITRIWPNTKYLD
Sbjct: 241 DISTATLNPKITDPCLRECIVSKYLTNPNPELAELISKECSTEEWEGIITRIWPNTKYLD 300

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360
           VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCY E
Sbjct: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCE 360

Query: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420
           FIPLDNDMSS PTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF
Sbjct: 361 FIPLDNDMSSSPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420

Query: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480
           VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL
Sbjct: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480

Query: 481 LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY 540
           LVKDEETGNFPPGEILNQ CLAIEESLNSVYRQGRVADNSIGPLEIRVVR GTFEELMDY
Sbjct: 481 LVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDY 540

Query: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG 589
           AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPE RG
Sbjct: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERRG 588

BLAST of Pay0001727 vs. ExPASy TrEMBL
Match: A0A5D3CNV8 (Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004400 PE=3 SV=1)

HSP 1 Score: 1036.9 bits (2680), Expect = 3.1e-299
Identity = 516/585 (88.21%), Postives = 537/585 (91.79%), Query Frame = 0

Query: 10  AKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKSNVRVVTYED 69
           AKAL+FIEE TTNAD VQQ+VLAEIL +N+ TEY+ RFRL+ +  RD FKS   VVTYED
Sbjct: 22  AKALRFIEETTTNADLVQQRVLAEILTQNAHTEYVNRFRLNGATDRDTFKSKFPVVTYED 81

Query: 70  LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQLLYSLLMPIM 129
           LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIK EMERRQLLYSLLMPIM
Sbjct: 82  LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIM 141

Query: 130 NKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPFNDYTSPNEA 189
           N YVPGLDKGKGLYFLFVKAETKTPGGL ARPVLTSYYKSDIF TRPYDPFNDYTSPNEA
Sbjct: 142 NLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEA 201

Query: 190 ILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAHDISTATLNP 249
           +LCA+SFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI FLQLNWKQLAHDIST TLNP
Sbjct: 202 VLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQLNWKQLAHDISTGTLNP 261

Query: 250 KITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQ 309
           KITD+CLRECI SKYLTNPN ELAE IS EC TEEWEGIITRIWPNTKYLDVIVTGAMAQ
Sbjct: 262 KITDTCLRECITSKYLTNPNPELAEFISNECYTEEWEGIITRIWPNTKYLDVIVTGAMAQ 321

Query: 310 YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFEFIPLDNDMS 369
           YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNM YFEF+P D+   
Sbjct: 322 YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMGYFEFLPHDSSSR 381

Query: 370 S------PPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFVRR 429
           +       P +LVDLADVEVGKEYELVITTY+GLCRYRVGD+L VTGFHNAAPQFRFVRR
Sbjct: 382 ASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLEVTGFHNAAPQFRFVRR 441

Query: 430 KNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWELLVK 489
           KNVLLSIDSDKTDEAELQKA++NA+ LLR FNT+VVEYTSYADTKTIPGHYVIYWELLVK
Sbjct: 442 KNVLLSIDSDKTDEAELQKAVENASSLLRDFNTTVVEYTSYADTKTIPGHYVIYWELLVK 501

Query: 490 DEETGN-FPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDYAI 549
           D ET N  P  EILNQ CLAIEESLNSVYRQGRVADNSIGPLEIRVVR GTFEELMDYAI
Sbjct: 502 DNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYAI 561

Query: 550 SRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPR 588
           SRGASINQYKAPRCVNFTPI+ELLDSRVTSVHFSPSKPHWTPE R
Sbjct: 562 SRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERR 606

BLAST of Pay0001727 vs. ExPASy TrEMBL
Match: A0A1S3B0G2 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=LOC103484816 PE=3 SV=1)

HSP 1 Score: 1036.9 bits (2680), Expect = 3.1e-299
Identity = 516/585 (88.21%), Postives = 537/585 (91.79%), Query Frame = 0

Query: 10  AKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKSNVRVVTYED 69
           AKAL+FIEE TTNAD VQQ+VLAEIL +N+ TEY+ RFRL+ +  RD FKS   VVTYED
Sbjct: 22  AKALRFIEETTTNADLVQQRVLAEILTQNAHTEYVNRFRLNGATDRDTFKSKFPVVTYED 81

Query: 70  LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQLLYSLLMPIM 129
           LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIK EMERRQLLYSLLMPIM
Sbjct: 82  LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIM 141

Query: 130 NKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPFNDYTSPNEA 189
           N YVPGLDKGKGLYFLFVKAETKTPGGL ARPVLTSYYKSDIF TRPYDPFNDYTSPNEA
Sbjct: 142 NLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEA 201

Query: 190 ILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAHDISTATLNP 249
           +LCA+SFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI FLQLNWKQLAHDIST TLNP
Sbjct: 202 VLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQLNWKQLAHDISTGTLNP 261

Query: 250 KITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQ 309
           KITD+CLRECI SKYLTNPN ELAE IS EC TEEWEGIITRIWPNTKYLDVIVTGAMAQ
Sbjct: 262 KITDTCLRECITSKYLTNPNPELAEFISNECYTEEWEGIITRIWPNTKYLDVIVTGAMAQ 321

Query: 310 YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFEFIPLDNDMS 369
           YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNM YFEF+P D+   
Sbjct: 322 YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMGYFEFLPHDSSSR 381

Query: 370 S------PPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFVRR 429
           +       P +LVDLADVEVGKEYELVITTY+GLCRYRVGD+L VTGFHNAAPQFRFVRR
Sbjct: 382 ASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLEVTGFHNAAPQFRFVRR 441

Query: 430 KNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWELLVK 489
           KNVLLSIDSDKTDEAELQKA++NA+ LLR FNT+VVEYTSYADTKTIPGHYVIYWELLVK
Sbjct: 442 KNVLLSIDSDKTDEAELQKAVENASSLLRDFNTTVVEYTSYADTKTIPGHYVIYWELLVK 501

Query: 490 DEETGN-FPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDYAI 549
           D ET N  P  EILNQ CLAIEESLNSVYRQGRVADNSIGPLEIRVVR GTFEELMDYAI
Sbjct: 502 DNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYAI 561

Query: 550 SRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPR 588
           SRGASINQYKAPRCVNFTPI+ELLDSRVTSVHFSPSKPHWTPE R
Sbjct: 562 SRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERR 606

BLAST of Pay0001727 vs. NCBI nr
Match: XP_008440334.1 (PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo])

HSP 1 Score: 1189.9 bits (3077), Expect = 0.0e+00
Identity = 588/588 (100.00%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS 60
           MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS
Sbjct: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS 60

Query: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL 120
           NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL
Sbjct: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL 120

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180
           LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF
Sbjct: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180

Query: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240
           NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH
Sbjct: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240

Query: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300
           DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD
Sbjct: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360
           VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE
Sbjct: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360

Query: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420
           FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF
Sbjct: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420

Query: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480
           VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL
Sbjct: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480

Query: 481 LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY 540
           LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY
Sbjct: 481 LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY 540

Query: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG 589
           AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG
Sbjct: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG 588

BLAST of Pay0001727 vs. NCBI nr
Match: TYK12849.1 (putative indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo var. makuwa])

HSP 1 Score: 1185.6 bits (3066), Expect = 0.0e+00
Identity = 586/588 (99.66%), Postives = 587/588 (99.83%), Query Frame = 0

Query: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS 60
           MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSR+AGRDEFKS
Sbjct: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRAAGRDEFKS 60

Query: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL 120
           NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIK EMERRQL
Sbjct: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180
           LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF
Sbjct: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180

Query: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240
           NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH
Sbjct: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240

Query: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300
           DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD
Sbjct: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360
           VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE
Sbjct: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360

Query: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420
           FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF
Sbjct: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420

Query: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480
           VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL
Sbjct: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480

Query: 481 LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY 540
           LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY
Sbjct: 481 LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY 540

Query: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG 589
           AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG
Sbjct: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG 588

BLAST of Pay0001727 vs. NCBI nr
Match: XP_004141994.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis sativus] >KGN48571.1 hypothetical protein Csa_003209 [Cucumis sativus])

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 571/588 (97.11%), Postives = 574/588 (97.62%), Query Frame = 0

Query: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS 60
           MA SSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRF+LSR+A RDEFKS
Sbjct: 1   MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFQLSRTASRDEFKS 60

Query: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL 120
           NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIK EMERRQL
Sbjct: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180
           LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKT GGL ARPVLTSYYKSDIF TRPYDPF
Sbjct: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTTGGLLARPVLTSYYKSDIFKTRPYDPF 180

Query: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240
           NDYTSPNEAILCANSFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAIHFLQ NWKQLAH
Sbjct: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH 240

Query: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300
           DISTATLNPKITD CLRECIVSKYLTNPN ELAELISKECSTEEWEGIITRIWPNTKYLD
Sbjct: 241 DISTATLNPKITDPCLRECIVSKYLTNPNPELAELISKECSTEEWEGIITRIWPNTKYLD 300

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360
           VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCY E
Sbjct: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCE 360

Query: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420
           FIPLDNDMSS PTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF
Sbjct: 361 FIPLDNDMSSSPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420

Query: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480
           VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL
Sbjct: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480

Query: 481 LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY 540
           LVKDEETGNFPPGEILNQ CLAIEESLNSVYRQGRVADNSIGPLEIRVVR GTFEELMDY
Sbjct: 481 LVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDY 540

Query: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG 589
           AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPE RG
Sbjct: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERRG 588

BLAST of Pay0001727 vs. NCBI nr
Match: XP_038881399.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Benincasa hispida])

HSP 1 Score: 1084.3 bits (2803), Expect = 0.0e+00
Identity = 541/587 (92.16%), Postives = 559/587 (95.23%), Query Frame = 0

Query: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS 60
           MA  SS+SHAKAL+FIEEITT+ADSVQQ+VLAEILN+N+DTEYL RFRLSR+AGRDEFKS
Sbjct: 1   MAVVSSLSHAKALRFIEEITTDADSVQQRVLAEILNQNADTEYLNRFRLSRTAGRDEFKS 60

Query: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL 120
            V VVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPTIK EMERRQL
Sbjct: 61  KVPVVTYEDLQPDIQRIANGDRSPIFSSRPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180
           LYSLLMPIMNK+VPGLDKGKGLYFLFVKAETKTPGGL ARPVLTSYYKSDIF TRPYDPF
Sbjct: 121 LYSLLMPIMNKFVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPF 180

Query: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240
           NDYTSPN+AILC NSFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAIHFLQLNWKQLAH
Sbjct: 181 NDYTSPNDAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240

Query: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300
           D++T TLNPKITDSCLRECI SKYLTNPN ELAE ISKECSTEEWEGIITRIWPNTKYLD
Sbjct: 241 DVATGTLNPKITDSCLRECITSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLD 300

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360
           VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPS+VTYTIMPNM YFE
Sbjct: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSEVTYTIMPNMGYFE 360

Query: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420
           F+P  +DMSS PT+LVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF
Sbjct: 361 FLP--HDMSSSPTRLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420

Query: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480
           VRRKNVLLSIDSDKTDEAELQKAIDNAALLL+GFNTSVVEYTSYADTKTIPGHYVIYWEL
Sbjct: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLKGFNTSVVEYTSYADTKTIPGHYVIYWEL 480

Query: 481 LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY 540
           LVKDE T NFP  EILN  CLAIEESLNSVYRQ RVADNSIGPLEIRVV+ GTFEELMDY
Sbjct: 481 LVKDEVT-NFPSREILNLCCLAIEESLNSVYRQCRVADNSIGPLEIRVVKNGTFEELMDY 540

Query: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPR 588
           AISRGASINQYKAPRCVNFTPI+ELLDSRVTSVHFSPSKPHWTPE R
Sbjct: 541 AISRGASINQYKAPRCVNFTPILELLDSRVTSVHFSPSKPHWTPERR 584

BLAST of Pay0001727 vs. NCBI nr
Match: XP_008440335.1 (PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo] >TYK12848.1 putative indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo var. makuwa])

HSP 1 Score: 1036.9 bits (2680), Expect = 6.5e-299
Identity = 516/585 (88.21%), Postives = 537/585 (91.79%), Query Frame = 0

Query: 10  AKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKSNVRVVTYED 69
           AKAL+FIEE TTNAD VQQ+VLAEIL +N+ TEY+ RFRL+ +  RD FKS   VVTYED
Sbjct: 22  AKALRFIEETTTNADLVQQRVLAEILTQNAHTEYVNRFRLNGATDRDTFKSKFPVVTYED 81

Query: 70  LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQLLYSLLMPIM 129
           LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIK EMERRQLLYSLLMPIM
Sbjct: 82  LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIM 141

Query: 130 NKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPFNDYTSPNEA 189
           N YVPGLDKGKGLYFLFVKAETKTPGGL ARPVLTSYYKSDIF TRPYDPFNDYTSPNEA
Sbjct: 142 NLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEA 201

Query: 190 ILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAHDISTATLNP 249
           +LCA+SFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI FLQLNWKQLAHDIST TLNP
Sbjct: 202 VLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQLNWKQLAHDISTGTLNP 261

Query: 250 KITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQ 309
           KITD+CLRECI SKYLTNPN ELAE IS EC TEEWEGIITRIWPNTKYLDVIVTGAMAQ
Sbjct: 262 KITDTCLRECITSKYLTNPNPELAEFISNECYTEEWEGIITRIWPNTKYLDVIVTGAMAQ 321

Query: 310 YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFEFIPLDNDMS 369
           YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNM YFEF+P D+   
Sbjct: 322 YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMGYFEFLPHDSSSR 381

Query: 370 S------PPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFVRR 429
           +       P +LVDLADVEVGKEYELVITTY+GLCRYRVGD+L VTGFHNAAPQFRFVRR
Sbjct: 382 ASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLEVTGFHNAAPQFRFVRR 441

Query: 430 KNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWELLVK 489
           KNVLLSIDSDKTDEAELQKA++NA+ LLR FNT+VVEYTSYADTKTIPGHYVIYWELLVK
Sbjct: 442 KNVLLSIDSDKTDEAELQKAVENASSLLRDFNTTVVEYTSYADTKTIPGHYVIYWELLVK 501

Query: 490 DEETGN-FPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDYAI 549
           D ET N  P  EILNQ CLAIEESLNSVYRQGRVADNSIGPLEIRVVR GTFEELMDYAI
Sbjct: 502 DNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYAI 561

Query: 550 SRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPR 588
           SRGASINQYKAPRCVNFTPI+ELLDSRVTSVHFSPSKPHWTPE R
Sbjct: 562 SRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERR 606

BLAST of Pay0001727 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 883.6 bits (2282), Expect = 8.5e-257
Identity = 435/579 (75.13%), Postives = 492/579 (84.97%), Query Frame = 0

Query: 10  AKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKSNVRVVTYED 69
           AKAL+FIEE+T NAD+VQ+ +LAEIL RN+DTEYL+RF L  +  RD FK+ + V+TYED
Sbjct: 20  AKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVITYED 79

Query: 70  LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQLLYSLLMPIM 129
           LQP+IQRIA+GDRSPI S+HPISEFLTSSGTSAGERKLMPTIK E++RRQLLYSLLMP+M
Sbjct: 80  LQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLMPVM 139

Query: 130 NKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPFNDYTSPNEA 189
           N YVPGLDKGKG+YFLFVK+ETKTPGGLPARPVLTSYYKS+ F +RPYDP+N YTSPNEA
Sbjct: 140 NLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSPNEA 199

Query: 190 ILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAHDISTATLNP 249
           ILC +SFQSMYTQMLCGLL R  VLRVGAVFASGLLRAI FLQL+W + AHDI    L+ 
Sbjct: 200 ILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGCLDS 259

Query: 250 KITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQ 309
           +ITD  +R+C+    +  P+  LAE I +EC ++ WE IITRIWPNTKYLDVIVTGAMAQ
Sbjct: 260 EITDPSIRQCMSG--ILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMAQ 319

Query: 310 YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFEFIPLDNDMS 369
           YIPTLE+YSGGLPMACTMYASSECYFG+NLNPM KPS+V+YTIMPNM YFEFIPL     
Sbjct: 320 YIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGG--- 379

Query: 370 SPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFVRRKNVLLS 429
              T+ V+L DV +GKEYELV+TTY+GLCRYRVGDIL VTGFHN+APQF FVRRKNVLLS
Sbjct: 380 ---TKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNVLLS 439

Query: 430 IDSDKTDEAELQKAIDNAALLL-RGFNTSVVEYTSYADTKTIPGHYVIYWELLVKDEETG 489
           IDSDKTDE+ELQKA++NA+ +L     + V EYTSYADT TIPGHYV+YWELLV+D    
Sbjct: 440 IDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRD--GA 499

Query: 490 NFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDYAISRGASI 549
             P  E L + CL +EESLNSVYRQ RVADNS+GPLEIRVVR GTFEELMDYAISRGASI
Sbjct: 500 RQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGASI 559

Query: 550 NQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPR 588
           NQYK PRCVNFTPI+ELLDSRV S HFSPS PHWTPE R
Sbjct: 560 NQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERR 588

BLAST of Pay0001727 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 874.8 bits (2259), Expect = 4.0e-254
Identity = 437/593 (73.69%), Postives = 499/593 (84.15%), Query Frame = 0

Query: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS 60
           ++A++S    KAL+FIEE+T N DSVQ+KVL EIL RNS+TEYLKRF L     R  FKS
Sbjct: 12  VSATTSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKS 71

Query: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL 120
            V VVTYEDL+P+IQRI+NGD SPI SSHPI+EFLTSSGTSAGERKLMPTI+ +++RRQL
Sbjct: 72  KVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQL 131

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180
           LYSLLMP+MN YVPGLDKGKGLYFLFVK+E+KT GGLPARPVLTSYYKSD F  RPYDP+
Sbjct: 132 LYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPY 191

Query: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240
           N YTSPNEAILC++S QSMY QMLCGLLMR +VLR+GAVFASGLLRAI FLQ NWK+LA 
Sbjct: 192 NVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELAR 251

Query: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300
           DIST TL+ +I D  ++   +SK LT P+ ELAE +   CS E WEGIIT+IWPNTKYLD
Sbjct: 252 DISTGTLSSRIFDPAIKN-RMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLD 311

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360
           VIVTGAMAQYIPTLE+YSGGLPMACTMYASSE YFG+NL PMCKPS+V+YTIMPNM YFE
Sbjct: 312 VIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFE 371

Query: 361 FIPLDND------MSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNA 420
           F+P ++D       S   T LV+LA+VEVGKEYELVITTY+GL RYRVGDIL VTGFHN+
Sbjct: 372 FLPHNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNS 431

Query: 421 APQFRFVRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHY 480
           APQF+F+RRKNVLLS++SDKTDEAELQKA++NA+ L     T V+EYTSYA+TKTIPGHY
Sbjct: 432 APQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHY 491

Query: 481 VIYWELLVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTF 540
           VIYWELL +D+        E++ + CL +EESLNSVYRQ RVAD SIGPLEIRVVR GTF
Sbjct: 492 VIYWELLGRDQSNA-LMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTF 551

Query: 541 EELMDYAISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPR 588
           EELMDYAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSPS PHW+PE R
Sbjct: 552 EELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPERR 602

BLAST of Pay0001727 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 865.9 bits (2236), Expect = 1.8e-251
Identity = 434/588 (73.81%), Postives = 498/588 (84.69%), Query Frame = 0

Query: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS 60
           M  S S    KAL+FIEE+T N D VQ+KV+ EIL+RNSDTEYLKRF L     R  FK+
Sbjct: 11  MMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKT 70

Query: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL 120
            V VV Y+DL+P+IQRIANGDRS I SS+PI+EFLTSSGTSAGERKLMPTI  +M+RRQL
Sbjct: 71  KVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQL 130

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180
           LYSLLMP+MN YVPGLDKGK LYFLFVK E+KTPGGLPARPVLTSYYKS+ F  RP DP+
Sbjct: 131 LYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPY 190

Query: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240
           N YTSPNEAILC +S QSMYTQMLCGLLMR +VLR+GAVFASGLLRAI FLQ NWK+LA 
Sbjct: 191 NVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELAD 250

Query: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTE-EWEGIITRIWPNTKYL 300
           DIST TL+ +I+D  ++E  +SK LT P+ ELA+ I+  C  +  WEGIIT+IWPNTKYL
Sbjct: 251 DISTGTLSSRISDPAIKES-MSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYL 310

Query: 301 DVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYF 360
           DVIVTGAMAQYIP LE+YSGGLPMACTMYASSE YFG+NL PMCKPS+V+YTIMPNM YF
Sbjct: 311 DVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYF 370

Query: 361 EFIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFR 420
           EF+P  +++ +  ++LV+LADVEVGKEYELVITTY+GL RYRVGDIL VTGF+N+APQF+
Sbjct: 371 EFLP-HHEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFK 430

Query: 421 FVRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWE 480
           FVRRKNVLLSI+SDKTDEAELQ A++NA+LLL    T V+EYTSYA+TKTIPGHYVIYWE
Sbjct: 431 FVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWE 490

Query: 481 LLVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMD 540
           LLVKD+   N P  E++ + CL +EESLNSVYRQ RVAD SIGPLEIRVV+ GTFEELMD
Sbjct: 491 LLVKDQT--NPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMD 550

Query: 541 YAISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPR 588
           YAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSP+ PHW+PE R
Sbjct: 551 YAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERR 594

BLAST of Pay0001727 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 825.5 bits (2131), Expect = 2.8e-239
Identity = 415/586 (70.82%), Postives = 482/586 (82.25%), Query Frame = 0

Query: 4   SSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKSNVR 63
           ++S +  KAL+FIEEIT N DSVQ+KVL EIL+RNS+TEYLKRF L+ +  R  FKS V 
Sbjct: 15  TTSETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVP 74

Query: 64  VVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQLLYS 123
           VV YEDL+ DIQRI+NGDRSPI SSHPI+EFLTSSGTSAGERKLMPTI+ ++ RRQLL +
Sbjct: 75  VVIYEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGN 134

Query: 124 LLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPFNDY 183
           LLMP+MN YVPGLDKGKGLYFLFVK+E+ T GGLPARP LTSYYKSD F T   D  + Y
Sbjct: 135 LLMPVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSD--SVY 194

Query: 184 TSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAHDIS 243
           TSP EAILC +S QSMYTQMLCGLLMR +V R+GAVF SGLLRAI FLQ NWK+L+ DIS
Sbjct: 195 TSPKEAILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDIS 254

Query: 244 TATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLDVIV 303
           T TL+ KI D  ++   +S  L  P+ ELAE +   CS E WEGIIT+IWPNTKYLDVIV
Sbjct: 255 TGTLSSKIFDHAIK-TRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIV 314

Query: 304 TGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFEFIP 363
           TGAMA+YIP LE+YSGGLPMA  +YASSE YFG+NLNPMCKPS+V+YTI PNM YFEF+P
Sbjct: 315 TGAMAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLP 374

Query: 364 --LDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFV 423
              D D     T LV+LADVEVGKEYELVITTY+GL RYRVGDIL VTGFHN+APQF+F+
Sbjct: 375 HNHDGDGGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFI 434

Query: 424 RRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWELL 483
           RR+NVLLSI+SDKTDEA+LQKA++NA+ LL    T V+EYTSYADTKTIPGHYVIYWELL
Sbjct: 435 RRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELL 494

Query: 484 VKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDYA 543
            +D+     P  E++ + CL +EESLN+VYRQ RV+D SIGPLEIRVV+ GTFEELMD++
Sbjct: 495 SRDQSNA-LPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDFS 554

Query: 544 ISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPR 588
           ISRG+SINQYK PRCV+ TPI++LLDSRV S HFSPS PHW+PE R
Sbjct: 555 ISRGSSINQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERR 596

BLAST of Pay0001727 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 774.2 bits (1998), Expect = 7.3e-224
Identity = 375/586 (63.99%), Postives = 461/586 (78.67%), Query Frame = 0

Query: 13  LQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKSNVRVVTYEDLQP 72
           LQFIE++TTNAD VQ++VL EIL+RN+D EYLKR  L     R+ FK  + VVTYED+QP
Sbjct: 25  LQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYEDIQP 84

Query: 73  DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQLLYSLLMPIMNKY 132
           +I RIANGD+S +  S+PISEFLTSSGTS GERKLMPTI+ E++RR LLYSLLMP+M+++
Sbjct: 85  EINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMDQF 144

Query: 133 VPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPFNDYTSPNEAILC 192
           VPGLDKGKG+YFLF+K+E+KTPGGLPARPVLTSYYKS  F  RPYDP+ +YTSPN+ ILC
Sbjct: 145 VPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQTILC 204

Query: 193 ANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAHDISTATLNPKIT 252
           ++S+QSMY+QMLCGL    +VLRVGAVFASG +RAI FL+ +W +LA DI T TL+ +IT
Sbjct: 205 SDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLSSEIT 264

Query: 253 DSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQYIP 312
           DS +RE +    +  P+ +LA+ +  EC    W+GIITR+WPNTKY+DVIVTG M+QYIP
Sbjct: 265 DSSVREAVGE--ILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQYIP 324

Query: 313 TLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFEFIP------LDN 372
           TL++YS GLP+ CTMYASSECYFGVNL P+CKPS+V+YT++PNM YFEF+P      + +
Sbjct: 325 TLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSGVTS 384

Query: 373 DMSSPPT-------QLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFR 432
            +S P         +LVDL DV++G+EYELV+TTY+GL RYRVGD+L V GF N APQF 
Sbjct: 385 SISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNAPQFS 444

Query: 433 FVRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWE 492
           F+ RKNV+LSIDSDKTDE ELQ A+ NA   L  F+ S+ EYTSYADT +IPGHYV++WE
Sbjct: 445 FICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWE 504

Query: 493 LLVKDEETGNFP-PGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELM 552
           L +     GN P P  +    CL IEESLNSVYRQGRV+D SIGPLEI++V  GTF++LM
Sbjct: 505 LCL----NGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKLM 564

Query: 553 DYAISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTP 585
           DYAIS GASINQYK PRCV F PIIELL+SRV   +FSP  P W+P
Sbjct: 565 DYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O823331.2e-25575.13Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=... [more]
O221902.6e-25073.81Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
A3BLS01.6e-24472.79Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399... [more]
Q0D4Z61.6e-24472.79Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=3... [more]
P0C0M21.2e-23969.01Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japo... [more]
Match NameE-valueIdentityDescription
A0A1S3B1M00.0e+00100.00probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=... [more]
A0A5D3CR820.0e+0099.66Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa... [more]
A0A0A0KLF80.0e+0097.11Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G492310 PE=3 SV=1[more]
A0A5D3CNV83.1e-29988.21Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa... [more]
A0A1S3B0G23.1e-29988.21probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=... [more]
Match NameE-valueIdentityDescription
XP_008440334.10.0e+00100.00PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo][more]
TYK12849.10.0e+0099.66putative indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo var. makuwa][more]
XP_004141994.10.0e+0097.11probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis sativus] >KGN48571... [more]
XP_038881399.10.0e+0092.16probable indole-3-acetic acid-amido synthetase GH3.1 [Benincasa hispida][more]
XP_008440335.16.5e-29988.21PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo] >... [more]
Match NameE-valueIdentityDescription
AT2G14960.18.5e-25775.13Auxin-responsive GH3 family protein [more]
AT4G37390.14.0e-25473.69Auxin-responsive GH3 family protein [more]
AT2G23170.11.8e-25173.81Auxin-responsive GH3 family protein [more]
AT1G59500.12.8e-23970.82Auxin-responsive GH3 family protein [more]
AT5G54510.17.3e-22463.99Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 16..559
e-value: 1.2E-198
score: 661.3
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 5..587
NoneNo IPR availablePANTHERPTHR31901:SF7INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.2-RELATEDcoord: 5..587

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0001727.1Pay0001727.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016881 acid-amino acid ligase activity