PI0028611 (gene) Melon (PI 482460) v1

BLAST of PI0028611 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 2.3e-171
Identity = 353/596 (59.23%), Postives = 433/596 (72.65%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKV-GSSNSLSDLEVAIVKATRHEEYPAEERHVREILSL 60
           M  SK +RA+GAVKD+TS+GLAKV G S SLS+L+VAIVKATRHEE+PAEE+++REILSL
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRF 120
           T YSR+YI+ACV+TLSRRLNKTK WTVALKTL+LIQRL+            F+TRRGTR 
Sbjct: 61  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHHQQQ 180
           LNMSDFRD S+SNSWDYSAFVRTYALYLDERL+FRMQ+R G+R  + +  E         
Sbjct: 121 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGE--------- 180

Query: 181 HINGSSSSNNNDDNNEEEDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTG 240
                  ++  + +    D S AIVVR+ PI +M +EQIF R QHLQQLL+RFLACRPTG
Sbjct: 181 -------ADEEEQDQAAADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTG 240

Query: 241 AAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCRVGKQFD 300
            A+NNRVV+VALYPIV+ESFQIYYD+TEIMGILI+RFMEL++ D +KVY+IFCRV KQF+
Sbjct: 241 NARNNRVVIVALYPIVKESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFE 300

Query: 301 ELELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSALAQCQKDREKTQPQLEI 360
           EL+ FYSWCK +GIARSSEYPE+EKIT KKL++MDEFI+DKSAL   ++ +       E 
Sbjct: 301 ELDQFYSWCKNMGIARSSEYPEIEKITQKKLDLMDEFIRDKSALEHTKQSKSVKSEADED 360

Query: 361 TEEEHKEE--EEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIKE------DEVKKDN 420
            +E   EE  EE+ DMN+IKALP P   +E++V   E  +EE  I++      D +   N
Sbjct: 361 DDEARTEEVNEEQEDMNAIKALPEPPPKEEDDVKPEEEAKEEVIIEKKQEEMGDLLDLGN 420

Query: 421 ENPVMGDLNADKLALALFDGSGPIDS-SVKALQWQAFNDDSADWETALVQSASNLSNQRA 480
            N        D LALALFDG     S S     W+AF DDSADWETALVQ+A+NLS Q++
Sbjct: 421 TNGGEAGQAGDSLALALFDGPYASGSGSESGPGWEAFKDDSADWETALVQTATNLSGQKS 480

Query: 481 DLGGGFDMLLLDGMYKQTTVMSTM-AGSGYGVSGSASSMALGSAGRPA------------ 540
           +LGGGFDMLLL+GMY+   V + +   + YG SGSASSMA GSAGRPA            
Sbjct: 481 ELGGGFDMLLLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATMLALPAPSTA 540

Query: 541 ------ISSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQ 558
                 I+S V  DPFAAS+ VAPP YVQM++ME+KQ++L+EEQ+MW QY+RDGRQ
Sbjct: 541 NGNAGNINSPVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSRDGRQ 580

BLAST of PI0028611 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 590.5 bits (1521), Expect = 2.0e-167
Identity = 344/598 (57.53%), Postives = 436/598 (72.91%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGS-SNSLSDLEVAIVKATRHEEYPAEERHVREILSL 60
           M  SK++RA+GAVKD+TS+GLAKVG  S+SL++LE+A+VKATRH++YPAE++++REIL L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRF 120
           T YSR Y+SACV TLSRRLNKTKNW+VALKTL+LIQRL+            F+TRRGTR 
Sbjct: 61  TSYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHHQQQ 180
           LNMSDFRD SQS+SWDYSAFVRTYALYLDERL++RMQ RRG++ + G    D        
Sbjct: 121 LNMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGD-------- 180

Query: 181 HINGSSSSNNNDDNNEEEDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTG 240
              G S   ++      +  SKAIVV++ P+ +M +E+IF+R QHLQQLL+RFLACRPTG
Sbjct: 181 ---GDSGEEDDHRGTSNDIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTG 240

Query: 241 AAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCRVGKQFD 300
            AKNNRVV+VA+YPIV+ESFQ+YY+ITEIMG+LI+RFMEL++ D +KVYEIFCRV KQFD
Sbjct: 241 NAKNNRVVIVAMYPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFD 300

Query: 301 ELELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSALAQCQKDREKTQPQLEI 360
           EL+ FY WCK + +ARSSEYPE+EKIT KKL++MDEFI+DKSALA  Q  +  ++   + 
Sbjct: 301 ELDPFYGWCKNMAVARSSEYPELEKITQKKLDLMDEFIRDKSALA-AQTTKSSSKRSNKS 360

Query: 361 TEEEHKEE---EEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIKEDEVKKDNENPVM 420
            EEE K E   E + D+NSIKALP PE  +EEE    E  E +K+++E   ++D E  ++
Sbjct: 361 EEEESKTEYIQENQEDLNSIKALPAPEQKEEEE--EEEKMETKKDVEEVVSRQDQEGDLL 420

Query: 421 GDLN----------ADKLALALFDGSGPIDSSVKALQWQAFNDDSADWETALVQSASNLS 480
            DL            D LALALFDG    +S+     W+AFND+SADWET LV+SA+ LS
Sbjct: 421 -DLTDEAGVTAGTVGDSLALALFDGVVGTESA-SGPGWEAFNDNSADWETDLVRSATRLS 480

Query: 481 NQRADLGGGFDMLLLDGMYKQTTVMSTM-AGSGYGVSGSASSMALGSAGRPAIS------ 540
            Q+++LGGGFD LLLDGMY+   V + +   + YG SGSASS+A GSAG PA S      
Sbjct: 481 GQKSELGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSASSVAFGSAGSPAASMLALPA 540

Query: 541 ----------SSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQ 558
                     S V+ DPFAAS+ VAPP YVQM++ME+KQ+LL+EEQ+MW QY R GRQ
Sbjct: 541 PPPTANGNRNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSGRQ 582

BLAST of PI0028611 vs. ExPASy Swiss-Prot
Match: Q8LF20 (Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=At2g25430 PE=1 SV=2)

HSP 1 Score: 432.2 bits (1110), Expect = 9.3e-120
Identity = 284/636 (44.65%), Postives = 386/636 (60.69%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPS IR+A+GAVKD+TSIG+AKV +SN   DLEVAIVKAT H++ PA E+++REIL+LT
Sbjct: 1   MAPS-IRKAIGAVKDQTSIGIAKV-ASNMAPDLEVAIVKATSHDDDPASEKYIREILNLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRFL 120
             SR YI ACV ++SRRL+KT++W VALK LML+ RL++          L+STRRGTR L
Sbjct: 61  SLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRML 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHHQQQH 180
           NMSDFRD + S+SWD+SAFVRTYA YLD+RLE  +  R+   S     +   H ++  ++
Sbjct: 121 NMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDDRY 180

Query: 181 ------------------INGSSSSNNNDDNN------------------------EEED 240
                               G   S+   DNN                           D
Sbjct: 181 GRGRDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGGGRD 240

Query: 241 NSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGAAKNNRVVLVALYPIVRES 300
             K +    TP+R+MT E+IF +  HLQ+LL+RFL+ RPTG AKN+R++L+ALYP+VRES
Sbjct: 241 EKKVV----TPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRES 300

Query: 301 FQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCRVGKQFDELELFYSWCKTIGIARSSE 360
           F++Y DI E++ +L+D+F ++   DCVK ++ +    KQ DEL  FY+WCK  G+ARSSE
Sbjct: 301 FKLYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSE 360

Query: 361 YPEVEKITPKKLEVMDEFIKDKSALAQCQKDREKTQPQLEITEEEHKEEEEEVDMNSIKA 420
           YPEV++IT K LE ++EF++D++     ++ +   + ++E      +EEE E DMN IKA
Sbjct: 361 YPEVQRITSKLLETLEEFVRDRA-----KRGKSPERKEIEAPPPVVEEEEPEPDMNEIKA 420

Query: 421 LPPPEDFKEEEVTAVEVKEEEKEIKEDEVKKDNENPVMGDLNADKLALALFDGSGPIDSS 480
           LPPPE++        E + E+ +  ED V    E+ V  D   +K ALALF  +GP  ++
Sbjct: 421 LPPPENYTPPPPPEPEPQPEKPQFTEDLVNL-REDEVTADDQGNKFALALF--AGPPGNN 480

Query: 481 VKALQWQAFNDD--------------SADWETALVQSASNLSNQRADLGGGFDMLLLDGM 540
            K   W+AF+ +               ADWE ALV++ SNL  Q A LGGGFD LLL+GM
Sbjct: 481 GK---WEAFSSNGVTSAWQNPAAEPGKADWELALVETTSNLEKQTAALGGGFDNLLLNGM 540

Query: 541 YKQTTVMSTMAGSGYGVSGSASSMALGSAGR--------PAISSSVV---SDPFAASVAV 560
           Y Q  V   ++ S     GSASS+AL   G+        PA   +V     DPFAAS+ +
Sbjct: 541 YDQGMVRQHVSTSQL-TGGSASSVALPLPGKTNNQVLALPAPDGTVEKVNQDPFAASLTI 600

BLAST of PI0028611 vs. ExPASy Swiss-Prot
Match: Q8S9J8 (Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=At4g32285 PE=1 SV=2)

HSP 1 Score: 429.1 bits (1102), Expect = 7.8e-119
Identity = 274/612 (44.77%), Postives = 387/612 (63.24%), Query Frame = 0

Query: 6   IRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLTCYSRA 65
           +R+A+G VKD+TSIG+AKV +SN   DLEVAIVKAT H++  + ++++REILSLT  SR 
Sbjct: 5   MRKAIGVVKDQTSIGIAKV-ASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 64

Query: 66  YISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRFLNMSDF 125
           Y+ ACV ++SRRL KT++W VALK LML+ RL++          L++TRRGTR LNMSDF
Sbjct: 65  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 124

Query: 126 RDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRR----SAFGLDDEDEHHHHQQQHI 185
           RD + S+SWD+SAFVRTYA YLD+RLE  +  RRGR     S+    + D+ ++  +   
Sbjct: 125 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDDF 184

Query: 186 -------------NG---SSSSNNNDDNNE--EEDNSKAIVVRATPIRDMTSEQIFSRTQ 245
                        NG      S +  D NE    +  K++    TP+R+MT E+IF +  
Sbjct: 185 RSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSV----TPLREMTPERIFGKMG 244

Query: 246 HLQQLLERFLACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQD 305
           HLQ+LL+RFL+CRPTG AKN+R++L+A+YP+V+ESF++Y DI E++ +L+D+F ++   D
Sbjct: 245 HLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTD 304

Query: 306 CVKVYEIFCRVGKQFDELELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSAL 365
           CVK ++ +    KQ DEL  FY WCK  G+ARSSEYPEV++IT K LE ++EF++D++  
Sbjct: 305 CVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKR 364

Query: 366 AQCQKDREKTQPQLEITEEEHKEEEEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIK 425
           A+  + +E     +E         EE VDMN IKALPPPE+       A E K ++ ++ 
Sbjct: 365 AKSPERKE-----IEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPAPEPKPQQPQVT 424

Query: 426 EDEVKKDNENPVMGDLNADKLALALFDGSGPIDSSVKALQWQAFNDDS------------ 485
           +D V    E+ V GD   +K ALALF  +GP  ++ K   W+AF+ D+            
Sbjct: 425 DDLVNL-REDDVSGDDQGNKFALALF--AGPPANNGK---WEAFSSDNNVTSAWQNPAAE 484

Query: 486 ---ADWETALVQSASNLSNQRADLGGGFDMLLLDGMYKQTTVMSTMAGSGYGVSGSASSM 545
              ADWE ALV++ASNL +Q+A +GGG D LLL+GMY Q  V   ++ S     GS+SS+
Sbjct: 485 LGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSEL-TGGSSSSV 544

Query: 546 ALGSAGR--------PAISSSVV---SDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLM 560
           AL   G+        PA   +V     DPFAAS+ + PP YVQM+EM++KQ LL +EQ +
Sbjct: 545 ALPLPGKVNSHILALPAPDGTVQKVNQDPFAASLTIPPPSYVQMAEMDKKQYLLTQEQQL 599

BLAST of PI0028611 vs. ExPASy Swiss-Prot
Match: Q9LVD8 (Putative clathrin assembly protein At5g57200 OS=Arabidopsis thaliana OX=3702 GN=At5g57200 PE=3 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 4.2e-56
Identity = 190/572 (33.22%), Postives = 300/572 (52.45%), Query Frame = 0

Query: 4   SKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT--C 63
           +  R+A GA+KD T++GLAKV S     DL++AIVKAT H E P +ERHVR+I S T   
Sbjct: 5   TSFRKAYGALKDTTTVGLAKVNS--EFKDLDIAIVKATNHVESPPKERHVRKIFSATSVI 64

Query: 64  YSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV---------DLFSTRRGTRFLNM 123
             RA ++ C++ LS+RL+KT+NW VA+K L++I R +         +L +       L +
Sbjct: 65  QPRADVAYCIHALSKRLSKTRNWVVAMKVLIVIHRTLREGDPTFREELLNYSHRRHILRI 124

Query: 124 SDFRDTSQSNSWDYSAFVRTYALYLDERLE-FRMQSRRGRRSAFGLDDEDEHHHHQQQHI 183
           S+F+D +   +WD SA+VRTYAL+L+ERLE +R+            D E E    +    
Sbjct: 125 SNFKDDTSPLAWDCSAWVRTYALFLEERLECYRV---------LKYDIEAE----RLPKA 184

Query: 184 NGSSSSNNNDDNNEEEDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGAA 243
           +G++S  +                     R ++ E +  +   LQQLL R + C+P GAA
Sbjct: 185 SGAASKTHR-------------------TRMLSGEDLLEQLPALQQLLYRLIGCQPEGAA 244

Query: 244 KNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCRVGKQFDEL 303
            +N ++  AL  +++ESF+IY  I + +  L+D F E++  D VK   I+ R G+Q + L
Sbjct: 245 YSNYLIQYALALVLKESFKIYCAINDGIINLVDMFFEMSRHDAVKALNIYKRAGQQAENL 304

Query: 304 ELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSALAQCQKD---REKTQPQLE 363
             FY +CK + +AR+ ++P + +  P  L  M+E+IK+       QK    +EK + + E
Sbjct: 305 AEFYDYCKGLELARNFQFPTLRQPPPSFLATMEEYIKEAPQSGSVQKKLEYQEKEEEEQE 364

Query: 364 ITEEEHKE--EEEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKE--IKEDEVKKDNE-N 423
             EE+ +E  EEE  + N+    P  E+ +EE    +EV+E +    I  D++   +E N
Sbjct: 365 QEEEQPEEPAEEENQNENTENDQPLIEEEEEEPKEEIEVEEAKPSPLIDTDDLLGLHEIN 424

Query: 424 PVMGDLNADK-LALALFDGSGPIDSSVKALQWQAFNDDSADWETALVQSASNLSNQR--- 483
           P   ++  +   +LA++      ++S  +          + WE ALV   +N +N     
Sbjct: 425 PKAAEIEQNNAFSLAIYPPGH--ETSAPSNSLSLIEAGGSGWELALVTPQNNNNNNNNPR 484

Query: 484 ----ADLGGGFDMLLLDGMYKQTTV--MSTMAGSGYGVSGSASSMALGSAGRPAISSSVV 543
                 LGGGFD LLLD +Y+  T      +  +GYG   +A   AL S+        V 
Sbjct: 485 PVIATKLGGGFDNLLLDSLYEDDTARRQIQLTNAGYGFGATAIPGALASSNPNPF--GVQ 537

Query: 544 SDPFAASVAVAPPPYVQMSEMERKQKLLVEEQ 546
            DPFA S  +APP  VQM+ M+++Q +++  Q
Sbjct: 545 QDPFAMSNNMAPPTNVQMA-MQQQQMMMMNNQ 537

BLAST of PI0028611 vs. ExPASy TrEMBL
Match: A0A5A7SK49 (Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002810 PE=4 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 1.8e-283
Identity = 532/609 (87.36%), Postives = 551/609 (90.48%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV            FSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHHQQQH 180
           NMSDFRD S SNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLD+EDEHHHHQQQ+
Sbjct: 121 NMSDFRDASLSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDEEDEHHHHQQQN 180

Query: 181 INGSSSSNNNDDNNEEEDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
              S+++N ++DNN+EEDNSKAIV+RATPIRDMTS+QIFSRTQHLQQLLERFLACRPTGA
Sbjct: 181 SCSSNNNNIDNDNNDEEDNSKAIVIRATPIRDMTSDQIFSRTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCRVGKQFDE 300
           AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMEL +QDCVKVYEIFCRVGKQFDE
Sbjct: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELKIQDCVKVYEIFCRVGKQFDE 300

Query: 301 LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSALAQCQKDREKTQPQLEIT 360
           LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKD+SALAQCQKDREK QPQLEI 
Sbjct: 301 LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDRSALAQCQKDREKAQPQLEIA 360

Query: 361 EEEHKEEEEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIKEDEVKKDNENPVMGDL- 420
           EEEHK EEEE DMNS+KALPPPED KEEE TA EVKEEEKEIKEDE+KKDNEN +MGDL 
Sbjct: 361 EEEHKVEEEEEDMNSVKALPPPEDVKEEETTATEVKEEEKEIKEDEMKKDNENSMMGDLL 420

Query: 421 ------------NADKLALALFDGSGPIDSSVKALQWQAFNDDSADWETALVQSASNLSN 480
                       NADKLALALFDGSGP+DSSVKAL WQAFND+SADWE+ALVQSASNLSN
Sbjct: 421 NLGEEHAMIREENADKLALALFDGSGPVDSSVKALPWQAFNDESADWESALVQSASNLSN 480

Query: 481 QRADLGGGFDMLLLDGMYKQTTVMSTMAGSGYGVSGSASSMALGSAGRPAI--------- 540
           QRADLGGGFDMLLLDGMYKQT V STMAGSGYGVSGSASSMALGSAGRPA+         
Sbjct: 481 QRADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAGRPAMLALPAPPTP 540

Query: 541 ------SSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNP 572
                 SSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNP
Sbjct: 541 EGSGTSSSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNP 600

BLAST of PI0028611 vs. ExPASy TrEMBL
Match: A0A0A0K783 (ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G058550 PE=4 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 1.8e-283
Identity = 535/618 (86.57%), Postives = 558/618 (90.29%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV            FSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHH---Q 180
           NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLD+EDEHHHH   Q
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDEEDEHHHHHQQQ 180

Query: 181 QQHINGSSSSNNND----DNNEEEDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFL 240
           QQHIN SSS+NN D    DNN+EED+SKAI+VRATP+RDMTSEQIFSRTQHLQQLLERFL
Sbjct: 181 QQHINSSSSNNNEDNNDNDNNDEEDHSKAIIVRATPMRDMTSEQIFSRTQHLQQLLERFL 240

Query: 241 ACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCR 300
           ACRPTGAAKNNRVVLVALYP+VRESFQIYYDITEIMGILIDRFM+LNVQDCVKVYEIFCR
Sbjct: 241 ACRPTGAAKNNRVVLVALYPVVRESFQIYYDITEIMGILIDRFMDLNVQDCVKVYEIFCR 300

Query: 301 VGKQFDELELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSALAQCQKDREKT 360
           VGKQFDELE+FYSWC+TIGIARS+EYPEVEKITPKKLEVMDEFIKD+SALAQCQ DREKT
Sbjct: 301 VGKQFDELEMFYSWCRTIGIARSAEYPEVEKITPKKLEVMDEFIKDRSALAQCQNDREKT 360

Query: 361 QPQLEITEEEHK--EEEEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIKEDEVKKDN 420
           QPQLEITEEEHK  EEEEEVD+NSIKALPPPED KEEE+TA+EV+EEEKEIKEDE+KKDN
Sbjct: 361 QPQLEITEEEHKEEEEEEEVDVNSIKALPPPEDIKEEEITAIEVEEEEKEIKEDEMKKDN 420

Query: 421 ENPVMGDL-------------NADKLALALFDGSGPIDSSVKALQWQAFNDDSADWETAL 480
           +N +MGDL             NADKLA ALFDGSGP+DSSVKAL WQAFNDDSADWETAL
Sbjct: 421 DNSMMGDLLNLGEEHAMIREANADKLATALFDGSGPVDSSVKALPWQAFNDDSADWETAL 480

Query: 481 VQSASNLSNQRADLGGGFDMLLLDGMYKQTTVMSTMAGSGYGVSGSASSMALGSAGRPAI 540
           VQSAS+L NQ+ADLGGGFDMLLLDGMYKQT V STMAGSGYGVSGSASSMALGSAGRPA+
Sbjct: 481 VQSASHLPNQKADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAGRPAM 540

Query: 541 ---------------SSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYAR 572
                          SSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYAR
Sbjct: 541 LALPAPTTPEGSGTSSSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYAR 600

BLAST of PI0028611 vs. ExPASy TrEMBL
Match: A0A1S3C1P9 (putative clathrin assembly protein At1g03050 OS=Cucumis melo OX=3656 GN=LOC103495455 PE=4 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 1.8e-283
Identity = 532/609 (87.36%), Postives = 551/609 (90.48%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV            FSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHHQQQH 180
           NMSDFRD S SNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLD+EDEHHHHQQQ+
Sbjct: 121 NMSDFRDASLSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDEEDEHHHHQQQN 180

Query: 181 INGSSSSNNNDDNNEEEDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
              S+++N ++DNN+EEDNSKAIV+RATPIRDMTS+QIFSRTQHLQQLLERFLACRPTGA
Sbjct: 181 SCSSNNNNIDNDNNDEEDNSKAIVIRATPIRDMTSDQIFSRTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCRVGKQFDE 300
           AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMEL +QDCVKVYEIFCRVGKQFDE
Sbjct: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELKIQDCVKVYEIFCRVGKQFDE 300

Query: 301 LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSALAQCQKDREKTQPQLEIT 360
           LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKD+SALAQCQKDREK QPQLEI 
Sbjct: 301 LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDRSALAQCQKDREKAQPQLEIA 360

Query: 361 EEEHKEEEEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIKEDEVKKDNENPVMGDL- 420
           EEEHK EEEE DMNS+KALPPPED KEEE TA EVKEEEKEIKEDE+KKDNEN +MGDL 
Sbjct: 361 EEEHKVEEEEEDMNSVKALPPPEDVKEEETTATEVKEEEKEIKEDEMKKDNENSMMGDLL 420

Query: 421 ------------NADKLALALFDGSGPIDSSVKALQWQAFNDDSADWETALVQSASNLSN 480
                       NADKLALALFDGSGP+DSSVKAL WQAFND+SADWE+ALVQSASNLSN
Sbjct: 421 NLGEEHAMIREENADKLALALFDGSGPVDSSVKALPWQAFNDESADWESALVQSASNLSN 480

Query: 481 QRADLGGGFDMLLLDGMYKQTTVMSTMAGSGYGVSGSASSMALGSAGRPAI--------- 540
           QRADLGGGFDMLLLDGMYKQT V STMAGSGYGVSGSASSMALGSAGRPA+         
Sbjct: 481 QRADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAGRPAMLALPAPPTP 540

Query: 541 ------SSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNP 572
                 SSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNP
Sbjct: 541 EGSGTSSSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNP 600

BLAST of PI0028611 vs. ExPASy TrEMBL
Match: A0A6J1HJ28 (putative clathrin assembly protein At1g03050 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464026 PE=4 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 4.2e-240
Identity = 466/616 (75.65%), Postives = 518/616 (84.09%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRFL 120
           CYS+AYISACVNTLSRRLNKTK+WTVALKTLMLIQRLV            FSTRRGTRFL
Sbjct: 61  CYSKAYISACVNTLSRRLNKTKDWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHHQQQH 180
           NMSDFRDTSQSNSWDYSAFVRTYALYLDERLE+RMQSRRGRRSAFGL +E++    QQQ+
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEYRMQSRRGRRSAFGLLEEED----QQQN 180

Query: 181 IN-GSSSSNNNDDNNEEEDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTG 240
            N  S+SSN N+D++++ DN+K I++RATP+ +MT+EQIFS+TQHLQQLLERFLACRPTG
Sbjct: 181 SNRNSNSSNTNNDDDDDNDNNKTIMIRATPLHEMTTEQIFSKTQHLQQLLERFLACRPTG 240

Query: 241 AAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCRVGKQFD 300
           AAK NRVVLVALYPIV+ESFQIYYDITE MGILIDRFM+L ++DCV+VYEIFCRVGKQFD
Sbjct: 241 AAKVNRVVLVALYPIVKESFQIYYDITETMGILIDRFMDLKIRDCVRVYEIFCRVGKQFD 300

Query: 301 ELELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSALAQCQKD-----REKTQ 360
           ELE+FYSWC+ IGIARS+EYPEVEKITPKKLEVMDEFIKDKSALAQC ++      E+ +
Sbjct: 301 ELEMFYSWCRNIGIARSTEYPEVEKITPKKLEVMDEFIKDKSALAQCLRNEVEITEEEEE 360

Query: 361 PQLEITEEEHKEEEEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIKEDEVKKDNENP 420
            + E  E+E +EEE+E DMNSIKALPPPE F+EE+   +  +E E    + + K D   P
Sbjct: 361 EEEEEEEDEDEEEEQEHDMNSIKALPPPESFEEEQTALLVNEENETNEMKAKEKSDEHYP 420

Query: 421 VMGDL-------------NADKLALALFDGSGP-IDSSVKALQWQAFNDDSADWETALVQ 480
           +MGD              NA+KLA ALFDGS P ID+SV AL W+AF+DD+ DWETALVQ
Sbjct: 421 MMGDFLNLDEEHQMTREENAEKLAFALFDGSAPIIDASVPALPWEAFSDDAPDWETALVQ 480

Query: 481 SASNLSNQRADLGGGFDMLLLDGMYKQTTVMSTMAGSGYGVSGSASSMALGSAGRPAI-- 540
           SASNLSNQR DLGGGFDMLLLDGMYKQTT MSTMAGSGYGVSGSASSMALGSAGRPA+  
Sbjct: 481 SASNLSNQRTDLGGGFDMLLLDGMYKQTTTMSTMAGSGYGVSGSASSMALGSAGRPALLA 540

Query: 541 -------------SSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDG 572
                        SSSV+SDPFAASVAVAPP YVQMSEMERK+KLLVEEQLMWQQYARDG
Sbjct: 541 LPAPSTSEGSVASSSSVMSDPFAASVAVAPPAYVQMSEMERKKKLLVEEQLMWQQYARDG 600

BLAST of PI0028611 vs. ExPASy TrEMBL
Match: A0A6J1DLL6 (putative clathrin assembly protein At1g03050 OS=Momordica charantia OX=3673 GN=LOC111021688 PE=4 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 5.6e-237
Identity = 468/613 (76.35%), Postives = 513/613 (83.69%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERH+REILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHIREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV----------DLFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV            FSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVLEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHHQQQH 180
           NMSDFRD SQS+SWDYSAFVRTYALYLDERLE+RMQSRRG+RSAFG++DE+E ++  Q +
Sbjct: 121 NMSDFRDVSQSDSWDYSAFVRTYALYLDERLEYRMQSRRGKRSAFGIEDEEEQNN--QNN 180

Query: 181 INGSSSSNNNDDNNEEEDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
           IN   ++NNN        NS+AIVVRA P+++MT+EQIFS+TQHLQQLLERFLACRPTGA
Sbjct: 181 INNIDTNNNN--------NSQAIVVRAAPVQEMTTEQIFSKTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCRVGKQFDE 300
           AKNNRVVLVALYP+V+ESFQIYYDITEIMGILIDRFMELN+ DCVKVYEIFCRVGKQFDE
Sbjct: 241 AKNNRVVLVALYPVVKESFQIYYDITEIMGILIDRFMELNIPDCVKVYEIFCRVGKQFDE 300

Query: 301 LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSALAQCQKDREKTQPQLEIT 360
           L+  YSWCKTIGIARSSEYPEVEKITPKKLEVMD+FIKDKSALAQC +DR     QL+IT
Sbjct: 301 LDQLYSWCKTIGIARSSEYPEVEKITPKKLEVMDDFIKDKSALAQCNRDRPNKPLQLQIT 360

Query: 361 EEE-----HKEEEEEVDMNSIKALPPPEDFK-EEEVTAVEVKEEEKEIKEDEVKKDNENP 420
           E+E      ++E E+ DMN+IKALPPPEDFK E+E  A+EVK EE E KEDE KKD ENP
Sbjct: 361 EQEEEDSKQEQEPEDQDMNAIKALPPPEDFKPEQEPAAIEVK-EESEPKEDEKKKD-ENP 420

Query: 421 VMGDL-------------NADKLALALFDGSGPIDSSVKALQWQAFNDDSADWETALVQS 480
           +M DL             NADKLALALFDGSGP   ++ A  WQAFN++++DWETALVQS
Sbjct: 421 MMCDLLNLGEDHAIMREENADKLALALFDGSGPKVEALPAPTWQAFNEETSDWETALVQS 480

Query: 481 ASNLSNQRADLGGGFDMLLLDGMYKQTTVMSTMAGSGYGVSGSASSMALGSAGRPAI--- 540
           ASNLS+QRA+LGGGFDMLLLDGMYKQ TV+     +G G SGSASSMALGSAGRPA+   
Sbjct: 481 ASNLSHQRAELGGGFDMLLLDGMYKQPTVV-----TGVGSSGSASSMALGSAGRPAMLAL 540

Query: 541 ----------SSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQI 572
                     +SS  SDPFAASVAVAPP YVQMSEMERKQKLLVEEQLMWQQYARDGRQI
Sbjct: 541 PAPPPPGEGGASSSSSDPFAASVAVAPPSYVQMSEMERKQKLLVEEQLMWQQYARDGRQI 596

BLAST of PI0028611 vs. NCBI nr
Match: XP_011658768.1 (putative clathrin assembly protein At1g03050 [Cucumis sativus] >KGN43681.1 hypothetical protein Csa_017299 [Cucumis sativus])

HSP 1 Score: 984.6 bits (2544), Expect = 3.7e-283
Identity = 535/618 (86.57%), Postives = 558/618 (90.29%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV            FSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHH---Q 180
           NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLD+EDEHHHH   Q
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDEEDEHHHHHQQQ 180

Query: 181 QQHINGSSSSNNND----DNNEEEDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFL 240
           QQHIN SSS+NN D    DNN+EED+SKAI+VRATP+RDMTSEQIFSRTQHLQQLLERFL
Sbjct: 181 QQHINSSSSNNNEDNNDNDNNDEEDHSKAIIVRATPMRDMTSEQIFSRTQHLQQLLERFL 240

Query: 241 ACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCR 300
           ACRPTGAAKNNRVVLVALYP+VRESFQIYYDITEIMGILIDRFM+LNVQDCVKVYEIFCR
Sbjct: 241 ACRPTGAAKNNRVVLVALYPVVRESFQIYYDITEIMGILIDRFMDLNVQDCVKVYEIFCR 300

Query: 301 VGKQFDELELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSALAQCQKDREKT 360
           VGKQFDELE+FYSWC+TIGIARS+EYPEVEKITPKKLEVMDEFIKD+SALAQCQ DREKT
Sbjct: 301 VGKQFDELEMFYSWCRTIGIARSAEYPEVEKITPKKLEVMDEFIKDRSALAQCQNDREKT 360

Query: 361 QPQLEITEEEHK--EEEEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIKEDEVKKDN 420
           QPQLEITEEEHK  EEEEEVD+NSIKALPPPED KEEE+TA+EV+EEEKEIKEDE+KKDN
Sbjct: 361 QPQLEITEEEHKEEEEEEEVDVNSIKALPPPEDIKEEEITAIEVEEEEKEIKEDEMKKDN 420

Query: 421 ENPVMGDL-------------NADKLALALFDGSGPIDSSVKALQWQAFNDDSADWETAL 480
           +N +MGDL             NADKLA ALFDGSGP+DSSVKAL WQAFNDDSADWETAL
Sbjct: 421 DNSMMGDLLNLGEEHAMIREANADKLATALFDGSGPVDSSVKALPWQAFNDDSADWETAL 480

Query: 481 VQSASNLSNQRADLGGGFDMLLLDGMYKQTTVMSTMAGSGYGVSGSASSMALGSAGRPAI 540
           VQSAS+L NQ+ADLGGGFDMLLLDGMYKQT V STMAGSGYGVSGSASSMALGSAGRPA+
Sbjct: 481 VQSASHLPNQKADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAGRPAM 540

Query: 541 ---------------SSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYAR 572
                          SSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYAR
Sbjct: 541 LALPAPTTPEGSGTSSSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYAR 600

BLAST of PI0028611 vs. NCBI nr
Match: XP_008455244.1 (PREDICTED: putative clathrin assembly protein At1g03050 [Cucumis melo] >KAA0031514.1 putative clathrin assembly protein [Cucumis melo var. makuwa] >TYK06967.1 putative clathrin assembly protein [Cucumis melo var. makuwa])

HSP 1 Score: 984.6 bits (2544), Expect = 3.7e-283
Identity = 532/609 (87.36%), Postives = 551/609 (90.48%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV            FSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHHQQQH 180
           NMSDFRD S SNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLD+EDEHHHHQQQ+
Sbjct: 121 NMSDFRDASLSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDEEDEHHHHQQQN 180

Query: 181 INGSSSSNNNDDNNEEEDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
              S+++N ++DNN+EEDNSKAIV+RATPIRDMTS+QIFSRTQHLQQLLERFLACRPTGA
Sbjct: 181 SCSSNNNNIDNDNNDEEDNSKAIVIRATPIRDMTSDQIFSRTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCRVGKQFDE 300
           AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMEL +QDCVKVYEIFCRVGKQFDE
Sbjct: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELKIQDCVKVYEIFCRVGKQFDE 300

Query: 301 LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSALAQCQKDREKTQPQLEIT 360
           LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKD+SALAQCQKDREK QPQLEI 
Sbjct: 301 LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDRSALAQCQKDREKAQPQLEIA 360

Query: 361 EEEHKEEEEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIKEDEVKKDNENPVMGDL- 420
           EEEHK EEEE DMNS+KALPPPED KEEE TA EVKEEEKEIKEDE+KKDNEN +MGDL 
Sbjct: 361 EEEHKVEEEEEDMNSVKALPPPEDVKEEETTATEVKEEEKEIKEDEMKKDNENSMMGDLL 420

Query: 421 ------------NADKLALALFDGSGPIDSSVKALQWQAFNDDSADWETALVQSASNLSN 480
                       NADKLALALFDGSGP+DSSVKAL WQAFND+SADWE+ALVQSASNLSN
Sbjct: 421 NLGEEHAMIREENADKLALALFDGSGPVDSSVKALPWQAFNDESADWESALVQSASNLSN 480

Query: 481 QRADLGGGFDMLLLDGMYKQTTVMSTMAGSGYGVSGSASSMALGSAGRPAI--------- 540
           QRADLGGGFDMLLLDGMYKQT V STMAGSGYGVSGSASSMALGSAGRPA+         
Sbjct: 481 QRADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAGRPAMLALPAPPTP 540

Query: 541 ------SSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNP 572
                 SSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNP
Sbjct: 541 EGSGTSSSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQHNP 600

BLAST of PI0028611 vs. NCBI nr
Match: XP_038888902.1 (putative clathrin assembly protein At1g03050 isoform X1 [Benincasa hispida])

HSP 1 Score: 934.9 bits (2415), Expect = 3.4e-268
Identity = 518/616 (84.09%), Postives = 542/616 (87.99%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV            FSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHHQQQH 180
           NMSDFRDTSQSNSWDYSAFVRT+ALYLDERLEFRMQSRRG+RSAFGLDDE+     QQQ 
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEE----GQQQQ 180

Query: 181 INGSSSSN----NNDDNNEEEDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACR 240
           IN ++++N    N+DDNN+E+DNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACR
Sbjct: 181 INNNNNNNNDNKNDDDNNDEDDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACR 240

Query: 241 PTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCRVGK 300
           PTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNV DCVKVYEIFCRVGK
Sbjct: 241 PTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGK 300

Query: 301 QFDELELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSALAQCQKDREKTQPQ 360
           QFDELELFYSWC+TIGIARSSEYP+VEKITPKKLEVMDEFIKDKSALAQCQ  R K Q Q
Sbjct: 301 QFDELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQ 360

Query: 361 LEITEEEHKEEEEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIKEDEVKKDNENPVM 420
           +EITEE  K  EEE+DMNSIKALPPPEDFKEE++TA+EVKEEE EIKED +KKD ENP++
Sbjct: 361 IEITEEVKK--EEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKD-ENPLI 420

Query: 421 GDL-------------NADKLALALFDGSGPI--DSSVKALQWQAFNDDSADWETALVQS 480
           GDL             NADKLALALFDG GP+   SSVK L WQAFNDDS DWET LVQS
Sbjct: 421 GDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQS 480

Query: 481 ASNLSNQRADLGGGFDMLLLDGMYKQTTVMSTMAGSGYGVSGSASSMALGSAGRPAI--- 540
           ASNLSNQRADLGGGFDMLLLDGMYKQTT+MSTMAGSGYGVSGSASSMALGSAGRPA+   
Sbjct: 481 ASNLSNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRPAMLAL 540

Query: 541 -------------SSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDG 572
                        SSSVVSDPFAAS+AVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDG
Sbjct: 541 PAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDG 600

BLAST of PI0028611 vs. NCBI nr
Match: XP_038888903.1 (putative clathrin assembly protein At1g03050 isoform X2 [Benincasa hispida])

HSP 1 Score: 920.6 bits (2378), Expect = 6.5e-264
Identity = 512/612 (83.66%), Postives = 531/612 (86.76%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLV            FSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHHQQQH 180
           NMSDFRDTSQSNSWDYSAFVRT+ALYLDERLEFRMQSRRG+RSAFGLDDE+     QQQ 
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDDEE----GQQQ- 180

Query: 181 INGSSSSNNNDDNNEEEDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
                       NN+E+DNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA
Sbjct: 181 -----------QNNDEDDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCRVGKQFDE 300
           AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNV DCVKVYEIFCRVGKQFDE
Sbjct: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDE 300

Query: 301 LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSALAQCQKDREKTQPQLEIT 360
           LELFYSWC+TIGIARSSEYP+VEKITPKKLEVMDEFIKDKSALAQCQ  R K Q Q+EIT
Sbjct: 301 LELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEIT 360

Query: 361 EEEHKEEEEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIKEDEVKKDNENPVMGDL- 420
           EE  K  EEE+DMNSIKALPPPEDFKEE++TA+EVKEEE EIKED +KKD ENP++GDL 
Sbjct: 361 EEVKK--EEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKD-ENPLIGDLL 420

Query: 421 ------------NADKLALALFDGSGPI--DSSVKALQWQAFNDDSADWETALVQSASNL 480
                       NADKLALALFDG GP+   SSVK L WQAFNDDS DWET LVQSASNL
Sbjct: 421 NLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQSASNL 480

Query: 481 SNQRADLGGGFDMLLLDGMYKQTTVMSTMAGSGYGVSGSASSMALGSAGRPAI------- 540
           SNQRADLGGGFDMLLLDGMYKQTT+MSTMAGSGYGVSGSASSMALGSAGRPA+       
Sbjct: 481 SNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRPAMLALPAPP 540

Query: 541 ---------SSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ 572
                    SSSVVSDPFAAS+AVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ
Sbjct: 541 TITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ 593

BLAST of PI0028611 vs. NCBI nr
Match: KAG6571699.1 (putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 840.9 bits (2171), Expect = 6.6e-240
Identity = 469/620 (75.65%), Postives = 518/620 (83.55%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRFL 120
           CYS+AYISACVNTLSRRLNKTK+WTVALKTLMLIQRLV            FSTRRGTRFL
Sbjct: 61  CYSKAYISACVNTLSRRLNKTKDWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHHQQQH 180
           NMSDFRDTSQSNSWDYSAFVRTYALYLDERLE+RMQSRRGRRSAFGL +E++    QQQ+
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEYRMQSRRGRRSAFGLLEEED----QQQN 180

Query: 181 IN-GSSSSNNNDDNNEEEDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTG 240
            N  S+SSN N+D++++ DN+K I++RATP+ +MT+EQIFS+TQHLQQLLERFLACRPTG
Sbjct: 181 SNRNSNSSNTNNDDDDDNDNNKTIMIRATPLHEMTTEQIFSKTQHLQQLLERFLACRPTG 240

Query: 241 AAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCRVGKQFD 300
           AAK NRVVLVALYPIV+ESFQIYYDITE MGILIDRFM+L ++DCV+VYEIFCRVGKQFD
Sbjct: 241 AAKVNRVVLVALYPIVKESFQIYYDITETMGILIDRFMDLKIRDCVRVYEIFCRVGKQFD 300

Query: 301 ELELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSALAQCQKDREKTQPQLEI 360
           ELE+FYSWC+ IGIARS+EYPEVEKITPKKLEVMDEFIKDKSALAQC ++      ++EI
Sbjct: 301 ELEMFYSWCRNIGIARSTEYPEVEKITPKKLEVMDEFIKDKSALAQCLRN------EVEI 360

Query: 361 TEEEHKEEEE---------EVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIKEDEVKKD 420
           TEEE +EEEE         E DMNSIKALPPPE F+EE+   +  +E E    + + K D
Sbjct: 361 TEEEEEEEEEEDEDEEEEQEHDMNSIKALPPPESFEEEQTALLVNEENETNEMKAKEKSD 420

Query: 421 NENPVMGDL-------------NADKLALALFDGSGP-IDSSVKALQWQAFNDDSADWET 480
              P+MGD              NA+KLA ALFDGS P ID+SV AL W+AF+DD+ DWET
Sbjct: 421 EHYPMMGDFLNLDEEHQMTREENAEKLAFALFDGSAPIIDASVPALPWEAFSDDAPDWET 480

Query: 481 ALVQSASNLSNQRADLGGGFDMLLLDGMYKQTTVMSTMAGSGYGVSGSASSMALGSAGRP 540
           ALVQSASNLSNQR DLGGGFDMLLLDGMYKQTT MSTMAGSGYGVSGSASSMALGSAGRP
Sbjct: 481 ALVQSASNLSNQRTDLGGGFDMLLLDGMYKQTTTMSTMAGSGYGVSGSASSMALGSAGRP 540

Query: 541 AI---------------SSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQY 572
           A+               SSSV+SDPFAASVAVAPP YVQMSEMERK+KLLVEEQLMWQQY
Sbjct: 541 ALLALPAPSTSEGSVASSSSVMSDPFAASVAVAPPAYVQMSEMERKKKLLVEEQLMWQQY 600

BLAST of PI0028611 vs. TAIR 10
Match: AT1G03050.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 603.6 bits (1555), Expect = 1.7e-172
Identity = 353/596 (59.23%), Postives = 433/596 (72.65%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKV-GSSNSLSDLEVAIVKATRHEEYPAEERHVREILSL 60
           M  SK +RA+GAVKD+TS+GLAKV G S SLS+L+VAIVKATRHEE+PAEE+++REILSL
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRF 120
           T YSR+YI+ACV+TLSRRLNKTK WTVALKTL+LIQRL+            F+TRRGTR 
Sbjct: 61  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHHQQQ 180
           LNMSDFRD S+SNSWDYSAFVRTYALYLDERL+FRMQ+R G+R  + +  E         
Sbjct: 121 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGE--------- 180

Query: 181 HINGSSSSNNNDDNNEEEDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTG 240
                  ++  + +    D S AIVVR+ PI +M +EQIF R QHLQQLL+RFLACRPTG
Sbjct: 181 -------ADEEEQDQAAADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTG 240

Query: 241 AAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCRVGKQFD 300
            A+NNRVV+VALYPIV+ESFQIYYD+TEIMGILI+RFMEL++ D +KVY+IFCRV KQF+
Sbjct: 241 NARNNRVVIVALYPIVKESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFE 300

Query: 301 ELELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSALAQCQKDREKTQPQLEI 360
           EL+ FYSWCK +GIARSSEYPE+EKIT KKL++MDEFI+DKSAL   ++ +       E 
Sbjct: 301 ELDQFYSWCKNMGIARSSEYPEIEKITQKKLDLMDEFIRDKSALEHTKQSKSVKSEADED 360

Query: 361 TEEEHKEE--EEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIKE------DEVKKDN 420
            +E   EE  EE+ DMN+IKALP P   +E++V   E  +EE  I++      D +   N
Sbjct: 361 DDEARTEEVNEEQEDMNAIKALPEPPPKEEDDVKPEEEAKEEVIIEKKQEEMGDLLDLGN 420

Query: 421 ENPVMGDLNADKLALALFDGSGPIDS-SVKALQWQAFNDDSADWETALVQSASNLSNQRA 480
            N        D LALALFDG     S S     W+AF DDSADWETALVQ+A+NLS Q++
Sbjct: 421 TNGGEAGQAGDSLALALFDGPYASGSGSESGPGWEAFKDDSADWETALVQTATNLSGQKS 480

Query: 481 DLGGGFDMLLLDGMYKQTTVMSTM-AGSGYGVSGSASSMALGSAGRPA------------ 540
           +LGGGFDMLLL+GMY+   V + +   + YG SGSASSMA GSAGRPA            
Sbjct: 481 ELGGGFDMLLLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATMLALPAPSTA 540

Query: 541 ------ISSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQ 558
                 I+S V  DPFAAS+ VAPP YVQM++ME+KQ++L+EEQ+MW QY+RDGRQ
Sbjct: 541 NGNAGNINSPVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSRDGRQ 580

BLAST of PI0028611 vs. TAIR 10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 590.5 bits (1521), Expect = 1.4e-168
Identity = 344/598 (57.53%), Postives = 436/598 (72.91%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGS-SNSLSDLEVAIVKATRHEEYPAEERHVREILSL 60
           M  SK++RA+GAVKD+TS+GLAKVG  S+SL++LE+A+VKATRH++YPAE++++REIL L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRF 120
           T YSR Y+SACV TLSRRLNKTKNW+VALKTL+LIQRL+            F+TRRGTR 
Sbjct: 61  TSYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHHQQQ 180
           LNMSDFRD SQS+SWDYSAFVRTYALYLDERL++RMQ RRG++ + G    D        
Sbjct: 121 LNMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGD-------- 180

Query: 181 HINGSSSSNNNDDNNEEEDNSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTG 240
              G S   ++      +  SKAIVV++ P+ +M +E+IF+R QHLQQLL+RFLACRPTG
Sbjct: 181 ---GDSGEEDDHRGTSNDIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTG 240

Query: 241 AAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCRVGKQFD 300
            AKNNRVV+VA+YPIV+ESFQ+YY+ITEIMG+LI+RFMEL++ D +KVYEIFCRV KQFD
Sbjct: 241 NAKNNRVVIVAMYPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFD 300

Query: 301 ELELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSALAQCQKDREKTQPQLEI 360
           EL+ FY WCK + +ARSSEYPE+EKIT KKL++MDEFI+DKSALA  Q  +  ++   + 
Sbjct: 301 ELDPFYGWCKNMAVARSSEYPELEKITQKKLDLMDEFIRDKSALA-AQTTKSSSKRSNKS 360

Query: 361 TEEEHKEE---EEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIKEDEVKKDNENPVM 420
            EEE K E   E + D+NSIKALP PE  +EEE    E  E +K+++E   ++D E  ++
Sbjct: 361 EEEESKTEYIQENQEDLNSIKALPAPEQKEEEE--EEEKMETKKDVEEVVSRQDQEGDLL 420

Query: 421 GDLN----------ADKLALALFDGSGPIDSSVKALQWQAFNDDSADWETALVQSASNLS 480
            DL            D LALALFDG    +S+     W+AFND+SADWET LV+SA+ LS
Sbjct: 421 -DLTDEAGVTAGTVGDSLALALFDGVVGTESA-SGPGWEAFNDNSADWETDLVRSATRLS 480

Query: 481 NQRADLGGGFDMLLLDGMYKQTTVMSTM-AGSGYGVSGSASSMALGSAGRPAIS------ 540
            Q+++LGGGFD LLLDGMY+   V + +   + YG SGSASS+A GSAG PA S      
Sbjct: 481 GQKSELGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSASSVAFGSAGSPAASMLALPA 540

Query: 541 ----------SSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQ 558
                     S V+ DPFAAS+ VAPP YVQM++ME+KQ+LL+EEQ+MW QY R GRQ
Sbjct: 541 PPPTANGNRNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSGRQ 582

BLAST of PI0028611 vs. TAIR 10
Match: AT2G25430.1 (epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related )

HSP 1 Score: 432.2 bits (1110), Expect = 6.6e-121
Identity = 284/636 (44.65%), Postives = 386/636 (60.69%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPS IR+A+GAVKD+TSIG+AKV +SN   DLEVAIVKAT H++ PA E+++REIL+LT
Sbjct: 1   MAPS-IRKAIGAVKDQTSIGIAKV-ASNMAPDLEVAIVKATSHDDDPASEKYIREILNLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRFL 120
             SR YI ACV ++SRRL+KT++W VALK LML+ RL++          L+STRRGTR L
Sbjct: 61  SLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRML 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDDEDEHHHHQQQH 180
           NMSDFRD + S+SWD+SAFVRTYA YLD+RLE  +  R+   S     +   H ++  ++
Sbjct: 121 NMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDDRY 180

Query: 181 ------------------INGSSSSNNNDDNN------------------------EEED 240
                               G   S+   DNN                           D
Sbjct: 181 GRGRDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGGGRD 240

Query: 241 NSKAIVVRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGAAKNNRVVLVALYPIVRES 300
             K +    TP+R+MT E+IF +  HLQ+LL+RFL+ RPTG AKN+R++L+ALYP+VRES
Sbjct: 241 EKKVV----TPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRES 300

Query: 301 FQIYYDITEIMGILIDRFMELNVQDCVKVYEIFCRVGKQFDELELFYSWCKTIGIARSSE 360
           F++Y DI E++ +L+D+F ++   DCVK ++ +    KQ DEL  FY+WCK  G+ARSSE
Sbjct: 301 FKLYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSE 360

Query: 361 YPEVEKITPKKLEVMDEFIKDKSALAQCQKDREKTQPQLEITEEEHKEEEEEVDMNSIKA 420
           YPEV++IT K LE ++EF++D++     ++ +   + ++E      +EEE E DMN IKA
Sbjct: 361 YPEVQRITSKLLETLEEFVRDRA-----KRGKSPERKEIEAPPPVVEEEEPEPDMNEIKA 420

Query: 421 LPPPEDFKEEEVTAVEVKEEEKEIKEDEVKKDNENPVMGDLNADKLALALFDGSGPIDSS 480
           LPPPE++        E + E+ +  ED V    E+ V  D   +K ALALF  +GP  ++
Sbjct: 421 LPPPENYTPPPPPEPEPQPEKPQFTEDLVNL-REDEVTADDQGNKFALALF--AGPPGNN 480

Query: 481 VKALQWQAFNDD--------------SADWETALVQSASNLSNQRADLGGGFDMLLLDGM 540
            K   W+AF+ +               ADWE ALV++ SNL  Q A LGGGFD LLL+GM
Sbjct: 481 GK---WEAFSSNGVTSAWQNPAAEPGKADWELALVETTSNLEKQTAALGGGFDNLLLNGM 540

Query: 541 YKQTTVMSTMAGSGYGVSGSASSMALGSAGR--------PAISSSVV---SDPFAASVAV 560
           Y Q  V   ++ S     GSASS+AL   G+        PA   +V     DPFAAS+ +
Sbjct: 541 YDQGMVRQHVSTSQL-TGGSASSVALPLPGKTNNQVLALPAPDGTVEKVNQDPFAASLTI 600

BLAST of PI0028611 vs. TAIR 10
Match: AT4G32285.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 429.1 bits (1102), Expect = 5.6e-120
Identity = 274/612 (44.77%), Postives = 387/612 (63.24%), Query Frame = 0

Query: 6   IRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLTCYSRA 65
           +R+A+G VKD+TSIG+AKV +SN   DLEVAIVKAT H++  + ++++REILSLT  SR 
Sbjct: 5   MRKAIGVVKDQTSIGIAKV-ASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 64

Query: 66  YISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRFLNMSDF 125
           Y+ ACV ++SRRL KT++W VALK LML+ RL++          L++TRRGTR LNMSDF
Sbjct: 65  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 124

Query: 126 RDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRR----SAFGLDDEDEHHHHQQQHI 185
           RD + S+SWD+SAFVRTYA YLD+RLE  +  RRGR     S+    + D+ ++  +   
Sbjct: 125 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDDF 184

Query: 186 -------------NG---SSSSNNNDDNNE--EEDNSKAIVVRATPIRDMTSEQIFSRTQ 245
                        NG      S +  D NE    +  K++    TP+R+MT E+IF +  
Sbjct: 185 RSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSV----TPLREMTPERIFGKMG 244

Query: 246 HLQQLLERFLACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQD 305
           HLQ+LL+RFL+CRPTG AKN+R++L+A+YP+V+ESF++Y DI E++ +L+D+F ++   D
Sbjct: 245 HLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTD 304

Query: 306 CVKVYEIFCRVGKQFDELELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSAL 365
           CVK ++ +    KQ DEL  FY WCK  G+ARSSEYPEV++IT K LE ++EF++D++  
Sbjct: 305 CVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKR 364

Query: 366 AQCQKDREKTQPQLEITEEEHKEEEEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIK 425
           A+  + +E     +E         EE VDMN IKALPPPE+       A E K ++ ++ 
Sbjct: 365 AKSPERKE-----IEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPAPEPKPQQPQVT 424

Query: 426 EDEVKKDNENPVMGDLNADKLALALFDGSGPIDSSVKALQWQAFNDDS------------ 485
           +D V    E+ V GD   +K ALALF  +GP  ++ K   W+AF+ D+            
Sbjct: 425 DDLVNL-REDDVSGDDQGNKFALALF--AGPPANNGK---WEAFSSDNNVTSAWQNPAAE 484

Query: 486 ---ADWETALVQSASNLSNQRADLGGGFDMLLLDGMYKQTTVMSTMAGSGYGVSGSASSM 545
              ADWE ALV++ASNL +Q+A +GGG D LLL+GMY Q  V   ++ S     GS+SS+
Sbjct: 485 LGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSEL-TGGSSSSV 544

Query: 546 ALGSAGR--------PAISSSVV---SDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLM 560
           AL   G+        PA   +V     DPFAAS+ + PP YVQM+EM++KQ LL +EQ +
Sbjct: 545 ALPLPGKVNSHILALPAPDGTVQKVNQDPFAASLTIPPPSYVQMAEMDKKQYLLTQEQQL 599

BLAST of PI0028611 vs. TAIR 10
Match: AT4G32285.2 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 429.1 bits (1102), Expect = 5.6e-120
Identity = 274/612 (44.77%), Postives = 387/612 (63.24%), Query Frame = 0

Query: 6   IRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLTCYSRA 65
           +R+A+G VKD+TSIG+AKV +SN   DLEVAIVKAT H++  + ++++REILSLT  SR 
Sbjct: 5   MRKAIGVVKDQTSIGIAKV-ASNMAPDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 64

Query: 66  YISACVNTLSRRLNKTKNWTVALKTLMLIQRLVD----------LFSTRRGTRFLNMSDF 125
           Y+ ACV ++SRRL KT++W VALK LML+ RL++          L++TRRGTR LNMSDF
Sbjct: 65  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 124

Query: 126 RDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRR----SAFGLDDEDEHHHHQQQHI 185
           RD + S+SWD+SAFVRTYA YLD+RLE  +  RRGR     S+    + D+ ++  +   
Sbjct: 125 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGYNRSRDDF 184

Query: 186 -------------NG---SSSSNNNDDNNE--EEDNSKAIVVRATPIRDMTSEQIFSRTQ 245
                        NG      S +  D NE    +  K++    TP+R+MT E+IF +  
Sbjct: 185 RSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSV----TPLREMTPERIFGKMG 244

Query: 246 HLQQLLERFLACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVQD 305
           HLQ+LL+RFL+CRPTG AKN+R++L+A+YP+V+ESF++Y DI E++ +L+D+F ++   D
Sbjct: 245 HLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTD 304

Query: 306 CVKVYEIFCRVGKQFDELELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDKSAL 365
           CVK ++ +    KQ DEL  FY WCK  G+ARSSEYPEV++IT K LE ++EF++D++  
Sbjct: 305 CVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKR 364

Query: 366 AQCQKDREKTQPQLEITEEEHKEEEEEVDMNSIKALPPPEDFKEEEVTAVEVKEEEKEIK 425
           A+  + +E     +E         EE VDMN IKALPPPE+       A E K ++ ++ 
Sbjct: 365 AKSPERKE-----IEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPAPEPKPQQPQVT 424

Query: 426 EDEVKKDNENPVMGDLNADKLALALFDGSGPIDSSVKALQWQAFNDDS------------ 485
           +D V    E+ V GD   +K ALALF  +GP  ++ K   W+AF+ D+            
Sbjct: 425 DDLVNL-REDDVSGDDQGNKFALALF--AGPPANNGK---WEAFSSDNNVTSAWQNPAAE 484

Query: 486 ---ADWETALVQSASNLSNQRADLGGGFDMLLLDGMYKQTTVMSTMAGSGYGVSGSASSM 545
              ADWE ALV++ASNL +Q+A +GGG D LLL+GMY Q  V   ++ S     GS+SS+
Sbjct: 485 LGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSEL-TGGSSSSV 544

Query: 546 ALGSAGR--------PAISSSVV---SDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLM 560
           AL   G+        PA   +V     DPFAAS+ + PP YVQM+EM++KQ LL +EQ +
Sbjct: 545 ALPLPGKVNSHILALPAPDGTVQKVNQDPFAASLTIPPPSYVQMAEMDKKQYLLTQEQQL 599