PI0028471 (gene) Melon (PI 482460) v1

Overview


Name	PI0028471
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Steroid 5-alpha-reductase DET2
Location	chr08: 22610463 .. 22611337 (-)
RNA-Seq Expression	PI0028471
Synteny	PI0028471

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGACATGGTACAAATTTGAAGACAGATCAGCCACAAATTTCTCAACTTGATTCAAACCTACAACCAGAATCCTCGAATCCATCGCTACGATGATGGGTTGGGATCAGGCTCTGTTCCATAACAGTCTTCTCGCCCTGTACCTCATTGGACCACCGACCGTCGTCGCCCTGAGGTTTCTCCAGGCGCCGTACGGGAAGCACATCCGACCGGGCTGGGGGCCGACCATGTCGGCTCCATTGGCCTGGTTCCTCATGGAAAGTCCCACCCTATGGTTGACCCTCCTACTCTTCCCTTCCGGCGATCACTCCTCTGATTCCAAGGCTCTCCTTCTCATCTCCCCCTTCCTCCTCCACTACCTCAATCGCACCTGCTTCTACCCGATTCGCCTTCTCTGGAGTGGGACTCGACGGAATGCCACGGCCGGTTTTCCGGTGATTGTGGCCGTCATGGCATTCGGGTTCAACTTGCTCAATTCTTACCTGCAGACGAGATCTGTGTCTCACTACGTGAAGTACGAGGGAGATGGGTGGTTTTGGTGGCGGTTTTTTGGTGGAGGAGTGGTTTTTGTGATGGGGATGGCGGTGAATATTTGGTCGGATGGTGTTCTGGTGAGACTGAAGAGGAGTGGCGGCGGTTATAAGGTGCCGACGGGAGGGTGGTTTGAGTTGGTGAGTTGCCCTAATTATTTGGGGGAGATTATGGAGTGGGTGGGCTGGGCGGTGATGTGCGGGTCATGGGCTGGGCTAGGGTTTTTTCTTTATACGTGTGCAAATTTAGTGCCTAGGGCACGTGCGAACCACACGTGGTACTTGAACAAGTTTGGAGAGGATTATCCCAGAAATAGGAAGGCTGTAATTCCCTTCTTGTATTAA

mRNA sequence

ATGAGACATGGTACAAATTTGAAGACAGATCAGCCACAAATTTCTCAACTTGATTCAAACCTACAACCAGAATCCTCGAATCCATCTCTTCTCGCCCTGTACCTCATTGGACCACCGACCGTCGTCGCCCTGAGGTTTCTCCAGGCGCCGTACGGGAAGCACATCCGACCGGGCTGGGGGCCGACCATGTCGGCTCCATTGGCCTGGTTCCTCATGGAAAGTCCCACCCTATGGTTGACCCTCCTACTCTTCCCTTCCGGCGATCACTCCTCTGATTCCAAGGCTCTCCTTCTCATCTCCCCCTTCCTCCTCCACTACCTCAATCGCACCTGCTTCTACCCGATTCGCCTTCTCTGGAGTGGGACTCGACGGAATGCCACGGCCGGTTTTCCGGTGATTGTGGCCGTCATGGCATTCGGGTTCAACTTGCTCAATTCTTACCTGCAGACGAGATCTGTGTCTCACTACGTGAAGTACGAGGGAGATGGGTGGTTTTGGTGGCGGTTTTTTGGTGGAGGAGTGGTTTTTGTGATGGGGATGGCGGTGAATATTTGGTCGGATGGTGTTCTGGTGAGACTGAAGAGGAGTGGCGGCGGTTATAAGGTGCCGACGGGAGGGTGGTTTGAGTTGGTGAGTTGCCCTAATTATTTGGGGGAGATTATGGAGTGGGTGGGCTGGGCGGTGATGTGCGGGTCATGGGCTGGGCTAGGGTTTTTTCTTTATACGTGTGCAAATTTAGTGCCTAGGGCACGTGCGAACCACACGTGGTACTTGAACAAGTTTGGAGAGGATTATCCCAGAAATAGGAAGGCTGTAATTCCCTTCTTGTATTAA

Coding sequence (CDS)

ATGAGACATGGTACAAATTTGAAGACAGATCAGCCACAAATTTCTCAACTTGATTCAAACCTACAACCAGAATCCTCGAATCCATCTCTTCTCGCCCTGTACCTCATTGGACCACCGACCGTCGTCGCCCTGAGGTTTCTCCAGGCGCCGTACGGGAAGCACATCCGACCGGGCTGGGGGCCGACCATGTCGGCTCCATTGGCCTGGTTCCTCATGGAAAGTCCCACCCTATGGTTGACCCTCCTACTCTTCCCTTCCGGCGATCACTCCTCTGATTCCAAGGCTCTCCTTCTCATCTCCCCCTTCCTCCTCCACTACCTCAATCGCACCTGCTTCTACCCGATTCGCCTTCTCTGGAGTGGGACTCGACGGAATGCCACGGCCGGTTTTCCGGTGATTGTGGCCGTCATGGCATTCGGGTTCAACTTGCTCAATTCTTACCTGCAGACGAGATCTGTGTCTCACTACGTGAAGTACGAGGGAGATGGGTGGTTTTGGTGGCGGTTTTTTGGTGGAGGAGTGGTTTTTGTGATGGGGATGGCGGTGAATATTTGGTCGGATGGTGTTCTGGTGAGACTGAAGAGGAGTGGCGGCGGTTATAAGGTGCCGACGGGAGGGTGGTTTGAGTTGGTGAGTTGCCCTAATTATTTGGGGGAGATTATGGAGTGGGTGGGCTGGGCGGTGATGTGCGGGTCATGGGCTGGGCTAGGGTTTTTTCTTTATACGTGTGCAAATTTAGTGCCTAGGGCACGTGCGAACCACACGTGGTACTTGAACAAGTTTGGAGAGGATTATCCCAGAAATAGGAAGGCTGTAATTCCCTTCTTGTATTAA

Protein sequence

MRHGTNLKTDQPQISQLDSNLQPESSNPSLLALYLIGPPTVVALRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAVMAFGFNLLNSYLQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVRLKRSGGGYKVPTGGWFELVSCPNYLGEIMEWVGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY

Homology

[Close]

BLAST of PI0028471 vs. ExPASy Swiss-Prot
Match: Q2QDF6 (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 5.9e-96
Identity = 170/250 (68.00%), Postives = 192/250 (76.80%), Query Frame = 0


Query: 30  LLALYLIGPPTVVALRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDH 89
           LL LY+I  PT ++L FLQAPYGKH RPGWGPT+S PLAWFLMESPTLWLT  LFPSG H
Sbjct: 12  LLTLYIIALPTWISLYFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLWLTFFLFPSGQH 71

Query: 90  SSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAVMAFGFNLLNSYLQ 149
             + K+ LLISPFL HY NRT  YP+RL  + T+   T GFPV VA MAFGFNLLN YLQ
Sbjct: 72  FYNPKSFLLISPFLFHYFNRTVLYPLRLARNTTQ---TRGFPVSVAFMAFGFNLLNGYLQ 131

Query: 150 TRSVSHYV-KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVRLKRSG-GGYKVPTGGW 209
            R VSHY   YE +  FWWRF  G ++FV+GM VN+ +D VLV LK+ G GGYK+P GG 
Sbjct: 132 ARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGLKKQGDGGYKIPRGGL 191

Query: 210 FELVSCPNYLGEIMEWVGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPR 269
           FELVSCPNY GEIMEW GWAVM  SW G GFFLYTCANL+PRARA   WYL KF +DYP+
Sbjct: 192 FELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARATRLWYLEKFKDDYPK 251

Query: 270 NRKAVIPFLY 278
           +RKAVIPF+Y
Sbjct: 252 DRKAVIPFIY 258

[Close]

BLAST of PI0028471 vs. ExPASy Swiss-Prot
Match: Q38944 (Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana OX=3702 GN=DET2 PE=1 SV=2)

HSP 1 Score: 345.5 bits (885), Expect = 5.5e-94
Identity = 161/250 (64.40%), Postives = 189/250 (75.60%), Query Frame = 0


Query: 30  LLALYLIGPPTVVALRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDH 89
           LL L   GPPT V L+FLQAPYGKH R GWGPT+S P+AWF+MESPTLWLTLLLFP G H
Sbjct: 14  LLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTLLLFPFGRH 73

Query: 90  SSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAVMAFGFNLLNSYLQ 149
           + + K+LLL SP+L+HY +RT  YP+RL  S        GFP+ +A +AF FNLLN Y+Q
Sbjct: 74  ALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKN-GFPITIAALAFTFNLLNGYIQ 133

Query: 150 TRSVSHYV-KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVRLKRSG-GGYKVPTGGW 209
            R VSHY   YE   WFWWRF  G VVF+ GM +NI SD  LVRLK+   GGY +P GGW
Sbjct: 134 ARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGW 193

Query: 210 FELVSCPNYLGEIMEWVGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPR 269
           FELVSCPNY GE +EW+GWAVM  SWAG+GFFLYTC+NL PRARA+H WY+ KF E+YP+
Sbjct: 194 FELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPK 253

Query: 270 NRKAVIPFLY 278
            RKAVIPF+Y
Sbjct: 254 TRKAVIPFVY 262

[Close]

BLAST of PI0028471 vs. ExPASy Swiss-Prot
Match: Q5K2N1 (Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum OX=4081 GN=DET2 PE=1 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 1.7e-82
Identity = 145/240 (60.42%), Postives = 176/240 (73.33%), Query Frame = 0


Query: 39  PTVVALRFLQAPYGKHIRPG-WGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALL 98
           PT +  +F  +PYGKH      G T+S P+AW  MESPTLWLT+++F  G + ++  A L
Sbjct: 22  PTFILCQFFTSPYGKHYTSADSGTTISPPIAWAFMESPTLWLTIIVFRLGKNYTNPLAFL 81

Query: 99  LISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAVMAFGFNLLNSYLQTRSVSHYV 158
           LISP+L HY NRT  YP+RL    T+ N    FP+ +AV AF FNLLN+Y+Q+R VSHY 
Sbjct: 82  LISPYLFHYTNRTIIYPLRLRSRNTKNN----FPLNIAVTAFIFNLLNAYIQSRWVSHYA 141

Query: 159 KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVRLKRSGGGYKVPTGGWFELVSCPNYL 218
            Y+ D WFW RF  G V+F  GM +NIW+DGVL+ LK  GGGYK+P GG F+ VS PNYL
Sbjct: 142 NYQEDDWFWVRFGIGLVIFGSGMLLNIWADGVLLGLKSQGGGYKIPRGGLFDYVSSPNYL 201

Query: 219 GEIMEWVGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 278
           GEIMEW+GWA+M  SWAGL FF+YTCANLVPRA +NH WYL KFGEDYP+NRKAV PFLY
Sbjct: 202 GEIMEWLGWALMTWSWAGLAFFVYTCANLVPRAVSNHKWYLQKFGEDYPKNRKAVFPFLY 257

[Close]

BLAST of PI0028471 vs. ExPASy Swiss-Prot
Match: Q68FF9 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 151.0 bits (380), Expect = 2.0e-35
Identity = 92/240 (38.33%), Postives = 135/240 (56.25%), Query Frame = 0


Query: 42  VALRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTL-W-LTLLLFPSGDHSSDSKALLLI 101
           V L+ + + YG++     G  + A  AWFL E P++ W L   + P+     +    +L+
Sbjct: 25  VGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSMAWPLYECIRPAAARLGNLPNRVLL 84

Query: 102 SPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIV--AVMAFGFNLLNSYLQTRSVSHYV 161
           + FL+HY+ RT  +P+ +           G P ++   V+AF F  LN YLQ+R +S + 
Sbjct: 85  AMFLIHYVQRTLVFPVLI---------RGGKPTLLFTFVLAFLFCTLNGYLQSRYLSQFA 144

Query: 162 KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVRLKRSG-GGYKVPTGGWFELVSCPNY 221
            Y  D      F  G  ++++GM +NI SD +L  L++ G  GYK+P GG FE VS  NY
Sbjct: 145 VYAEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSSANY 204

Query: 222 LGEIMEWVGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFL 277
            GE++EW G+A+   S  G+ F L+T   L  RAR +H WYL KF EDYP+ RK +IPFL
Sbjct: 205 FGELVEWCGFALASWSLQGVVFALFTLCALFTRARQHHQWYLEKF-EDYPKTRKILIPFL 254

[Close]

BLAST of PI0028471 vs. ExPASy Swiss-Prot
Match: P24008 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 151.0 bits (380), Expect = 2.0e-35
Identity = 90/243 (37.04%), Postives = 141/243 (58.02%), Query Frame = 0


Query: 40  TVVALRFLQAPYGKHIRPGW-GPTMSAPLAWFLMESPTL-W-LTLLLFPSGDHSSDSKAL 99
           ++V LR + +PYG++  P W G  + A  AWF+ E P++ W L   + P+     +    
Sbjct: 27  SIVGLRSVGSPYGRY-SPQWPGIRVPARPAWFIQELPSMAWPLYEYIRPAAARLGNLPNR 86

Query: 100 LLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVA--VMAFGFNLLNSYLQTRSVS 159
           +L++ FL+HY+ RT  +P+ +           G P ++   V+AF F   N Y+Q+R +S
Sbjct: 87  VLLAMFLIHYVQRTLVFPVLI---------RGGKPTLLVTFVLAFLFCTFNGYVQSRYLS 146

Query: 160 HYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVRLKRSG-GGYKVPTGGWFELVSC 219
            +  Y  D      F  G  ++++GM +NI SD +L  L++ G  GYK+P GG FE VS 
Sbjct: 147 QFAVYAEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSA 206

Query: 220 PNYLGEIMEWVGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVI 277
            NY GE++EW G+A+   S  G+ F L+T + L+ RA+ +H WY  KF EDYP++RK +I
Sbjct: 207 ANYFGELVEWCGFALASWSLQGVVFALFTLSTLLTRAKQHHQWYHEKF-EDYPKSRKILI 258

[Close]

BLAST of PI0028471 vs. ExPASy TrEMBL
Match: A0A1S3B6E3 (Steroid 5-alpha-reductase DET2 OS=Cucumis melo OX=3656 GN=LOC103486328 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 5.3e-156
Identity = 266/291 (91.41%), Postives = 272/291 (93.47%), Query Frame = 0


Query: 1   MRHGTNLKTDQPQISQLDSNLQPESSNP--------------SLLALYLIGPPTVVALRF 60
           MR+ TNLKTDQPQISQLDSNLQPESSNP              SLLALYLIGPPTVV+LRF
Sbjct: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAVMAFGFNLLNSYLQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSGTRRNATAGFPVIVA+MAFGFNLLN+Y+QTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVRLKRSGGGYKVPTGGWFELVSCPNYLGEIMEWVGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLV LKRSGGGYKVP GGWFELVSCPNYLGEIMEW+GW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 278
            VMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP NRKAVIPFLY
Sbjct: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY 291

[Close]

BLAST of PI0028471 vs. ExPASy TrEMBL
Match: A0A0A0LAB3 (Steroid 5-alpha-reductase DET2 OS=Cucumis sativus OX=3659 GN=Csa_3G732550 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.0e-154
Identity = 264/291 (90.72%), Postives = 269/291 (92.44%), Query Frame = 0


Query: 1   MRHGTNLKTDQPQISQLDSNLQPESSNP--------------SLLALYLIGPPTVVALRF 60
           MRHGTNLKTD PQISQLDSNLQPESSNP              SLLALYLIGPPTVV+LRF
Sbjct: 1   MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSS SKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAVMAFGFNLLNSYLQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSGTRRNA  GFPV+VA+MAFGFNLLNSY+QTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNAQVGFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVRLKRSGGGYKVPTGGWFELVSCPNYLGEIMEWVGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLV LKR GGGYKVPTGGWFELVSCPNYLGEIMEW+GW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 278
           AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGE YP NRKAVIPFLY
Sbjct: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 291

[Close]

BLAST of PI0028471 vs. ExPASy TrEMBL
Match: A0A5D3DNY2 (Steroid 5-alpha-reductase DET2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001080 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 6.1e-144
Identity = 240/249 (96.39%), Postives = 245/249 (98.39%), Query Frame = 0


Query: 29  SLLALYLIGPPTVVALRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGD 88
           SLLALYLIGPPTVV+LRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGD
Sbjct: 11  SLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGD 70

Query: 89  HSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAVMAFGFNLLNSYL 148
           HSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVA+MAFGFNLLN+Y+
Sbjct: 71  HSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYV 130

Query: 149 QTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVRLKRSGGGYKVPTGGWF 208
           QTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLV LKRSGGGYKVP GGWF
Sbjct: 131 QTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWF 190

Query: 209 ELVSCPNYLGEIMEWVGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRN 268
           ELVSCPNYLGEIMEW+GW VMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP N
Sbjct: 191 ELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPIN 250

Query: 269 RKAVIPFLY 278
           RKAVIPFLY
Sbjct: 251 RKAVIPFLY 259

[Close]

BLAST of PI0028471 vs. ExPASy TrEMBL
Match: A0A6J1J7F2 (Steroid 5-alpha-reductase DET2 OS=Cucurbita maxima OX=3661 GN=LOC111481932 PE=3 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 2.9e-130
Identity = 218/249 (87.55%), Postives = 234/249 (93.98%), Query Frame = 0


Query: 29  SLLALYLIGPPTVVALRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGD 88
           SLLALYLIGPPT ++LRFLQAPYGKH RPGWGPTMS PLAWFLMESPTLWLTLLLFPSGD
Sbjct: 11  SLLALYLIGPPTAISLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLWLTLLLFPSGD 70

Query: 89  HSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAVMAFGFNLLNSYL 148
           +SS+ K+LLLIS FLLHYLNRTC YPIRLL SGTRRN+  GFPV+VA+MAF FNLLNSYL
Sbjct: 71  NSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVVALMAFVFNLLNSYL 130

Query: 149 QTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVRLKRSGGGYKVPTGGWF 208
           QTRSVSHY+ Y+ D WFWWRFFGG VVFV+GMAVNIWSDGVLV+LK +GGGYKVPTGGWF
Sbjct: 131 QTRSVSHYMDYDRDEWFWWRFFGGAVVFVLGMAVNIWSDGVLVKLKMAGGGYKVPTGGWF 190

Query: 209 ELVSCPNYLGEIMEWVGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRN 268
           ELVSCPNYLGEI+EW+GWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPR+
Sbjct: 191 ELVSCPNYLGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRD 250

Query: 269 RKAVIPFLY 278
           RKAVIPFLY
Sbjct: 251 RKAVIPFLY 259

[Close]

BLAST of PI0028471 vs. ExPASy TrEMBL
Match: A0A6J1F1U8 (Steroid 5-alpha-reductase DET2 OS=Cucurbita moschata OX=3662 GN=LOC111441400 PE=3 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 1.9e-129
Identity = 217/249 (87.15%), Postives = 233/249 (93.57%), Query Frame = 0


Query: 29  SLLALYLIGPPTVVALRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGD 88
           SLLALYLIGPPT V+LRFLQAPYGKH RPGWGPTMS PLAWFLMESPTLWLTLLLFPSGD
Sbjct: 11  SLLALYLIGPPTAVSLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLWLTLLLFPSGD 70

Query: 89  HSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAVMAFGFNLLNSYL 148
           +SS+ K+LLLIS FLLHYLNRTC YPIRLL SGTRRN+  GFPV++A+MAF FNLLNSYL
Sbjct: 71  NSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVMALMAFVFNLLNSYL 130

Query: 149 QTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVRLKRSGGGYKVPTGGWF 208
           QTRSVSHY+ Y+ D WFWWRFFGG VVF++GMAVNIWSDGVLV+LK +GGGYKVPTGGWF
Sbjct: 131 QTRSVSHYMDYDRDEWFWWRFFGGAVVFLLGMAVNIWSDGVLVKLKMAGGGYKVPTGGWF 190

Query: 209 ELVSCPNYLGEIMEWVGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRN 268
           ELVSCPNYLGEI EW+GWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPR+
Sbjct: 191 ELVSCPNYLGEIAEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRD 250

Query: 269 RKAVIPFLY 278
           RKAVIPFLY
Sbjct: 251 RKAVIPFLY 259

[Close]

BLAST of PI0028471 vs. NCBI nr
Match: XP_008442471.1 (PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis melo])

HSP 1 Score: 560.1 bits (1442), Expect = 1.1e-155
Identity = 266/291 (91.41%), Postives = 272/291 (93.47%), Query Frame = 0


Query: 1   MRHGTNLKTDQPQISQLDSNLQPESSNP--------------SLLALYLIGPPTVVALRF 60
           MR+ TNLKTDQPQISQLDSNLQPESSNP              SLLALYLIGPPTVV+LRF
Sbjct: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAVMAFGFNLLNSYLQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSGTRRNATAGFPVIVA+MAFGFNLLN+Y+QTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVRLKRSGGGYKVPTGGWFELVSCPNYLGEIMEWVGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLV LKRSGGGYKVP GGWFELVSCPNYLGEIMEW+GW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 278
            VMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP NRKAVIPFLY
Sbjct: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY 291

[Close]

BLAST of PI0028471 vs. NCBI nr
Match: XP_004137737.1 (steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN58798.1 hypothetical protein Csa_002591 [Cucumis sativus])

HSP 1 Score: 555.8 bits (1431), Expect = 2.1e-154
Identity = 264/291 (90.72%), Postives = 269/291 (92.44%), Query Frame = 0


Query: 1   MRHGTNLKTDQPQISQLDSNLQPESSNP--------------SLLALYLIGPPTVVALRF 60
           MRHGTNLKTD PQISQLDSNLQPESSNP              SLLALYLIGPPTVV+LRF
Sbjct: 1   MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSS SKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAVMAFGFNLLNSYLQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSGTRRNA  GFPV+VA+MAFGFNLLNSY+QTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNAQVGFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVRLKRSGGGYKVPTGGWFELVSCPNYLGEIMEWVGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLV LKR GGGYKVPTGGWFELVSCPNYLGEIMEW+GW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 278
           AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGE YP NRKAVIPFLY
Sbjct: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 291

[Close]

BLAST of PI0028471 vs. NCBI nr
Match: KAA0044147.1 (steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa] >TYK24990.1 steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa])

HSP 1 Score: 520.0 bits (1338), Expect = 1.3e-143
Identity = 240/249 (96.39%), Postives = 245/249 (98.39%), Query Frame = 0


Query: 29  SLLALYLIGPPTVVALRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGD 88
           SLLALYLIGPPTVV+LRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGD
Sbjct: 11  SLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGD 70

Query: 89  HSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAVMAFGFNLLNSYL 148
           HSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVA+MAFGFNLLN+Y+
Sbjct: 71  HSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYV 130

Query: 149 QTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVRLKRSGGGYKVPTGGWF 208
           QTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLV LKRSGGGYKVP GGWF
Sbjct: 131 QTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWF 190

Query: 209 ELVSCPNYLGEIMEWVGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRN 268
           ELVSCPNYLGEIMEW+GW VMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP N
Sbjct: 191 ELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPIN 250

Query: 269 RKAVIPFLY 278
           RKAVIPFLY
Sbjct: 251 RKAVIPFLY 259

[Close]

BLAST of PI0028471 vs. NCBI nr
Match: XP_038905208.1 (LOW QUALITY PROTEIN: steroid 5-alpha-reductase DET2 [Benincasa hispida])

HSP 1 Score: 489.6 bits (1259), Expect = 1.8e-134
Identity = 227/249 (91.16%), Postives = 236/249 (94.78%), Query Frame = 0


Query: 29  SLLALYLIGPPTVVALRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGD 88
           SLL LYLIGP  VVALRFLQAPYGKH +PGWGPTMSAPLAWFLMESPTLWLTLLLFPSGD
Sbjct: 11  SLLRLYLIGPRQVVALRFLQAPYGKHNQPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGD 70

Query: 89  HSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAVMAFGFNLLNSYL 148
           HSS+SKALLLISPFLLHY NRTC YPIRLL SGTRRN  AGFPVIVAVMAFGFNLLNSYL
Sbjct: 71  HSSNSKALLLISPFLLHYFNRTCLYPIRLLRSGTRRNTAAGFPVIVAVMAFGFNLLNSYL 130

Query: 149 QTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVRLKRSGGGYKVPTGGWF 208
           QTRSVSHY++YEGDGWFWWRFFGGGVVF++GM+VNIWSDGVLV LKR GGGYKVPTG WF
Sbjct: 131 QTRSVSHYMEYEGDGWFWWRFFGGGVVFLLGMSVNIWSDGVLVGLKRGGGGYKVPTGWWF 190

Query: 209 ELVSCPNYLGEIMEWVGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRN 268
           ELVSCPNYLGEI+EW+GWAVMCGSWAGL FFLYTCANLVPRARANHTWYLNKFGEDYPRN
Sbjct: 191 ELVSCPNYLGEIVEWLGWAVMCGSWAGLAFFLYTCANLVPRARANHTWYLNKFGEDYPRN 250

Query: 269 RKAVIPFLY 278
           RKAVIPFLY
Sbjct: 251 RKAVIPFLY 259

[Close]

BLAST of PI0028471 vs. NCBI nr
Match: XP_023527474.1 (steroid 5-alpha-reductase DET2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 479.6 bits (1233), Expect = 1.9e-131
Identity = 221/249 (88.76%), Postives = 235/249 (94.38%), Query Frame = 0


Query: 29  SLLALYLIGPPTVVALRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGD 88
           SLLALYLIGPPT V+LRFLQAPYGKH RPGWGPTMS PLAWFLMESPTLWLTLLLFPSGD
Sbjct: 11  SLLALYLIGPPTAVSLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLWLTLLLFPSGD 70

Query: 89  HSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAVMAFGFNLLNSYL 148
           +SS+ K+LLLIS FLLHYLNRTC YPIRLL SGTRRN+  GFPV+VA+MAFGFNLLNSYL
Sbjct: 71  NSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVVALMAFGFNLLNSYL 130

Query: 149 QTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVRLKRSGGGYKVPTGGWF 208
           QTRSVSHYV Y+ D WFWWRFFGG VVFV+GMAVNIWSDGVLV+LK +GGGYKVPTGGWF
Sbjct: 131 QTRSVSHYVDYDRDEWFWWRFFGGAVVFVLGMAVNIWSDGVLVKLKMAGGGYKVPTGGWF 190

Query: 209 ELVSCPNYLGEIMEWVGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRN 268
           ELVSCPNYLGEI+EW+GWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPR+
Sbjct: 191 ELVSCPNYLGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRD 250

Query: 269 RKAVIPFLY 278
           RKAVIPFLY
Sbjct: 251 RKAVIPFLY 259

[Close]

BLAST of PI0028471 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 345.5 bits (885), Expect = 3.9e-95
Identity = 161/250 (64.40%), Postives = 189/250 (75.60%), Query Frame = 0


Query: 30  LLALYLIGPPTVVALRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDH 89
           LL L   GPPT V L+FLQAPYGKH R GWGPT+S P+AWF+MESPTLWLTLLLFP G H
Sbjct: 14  LLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTLLLFPFGRH 73

Query: 90  SSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAVMAFGFNLLNSYLQ 149
           + + K+LLL SP+L+HY +RT  YP+RL  S        GFP+ +A +AF FNLLN Y+Q
Sbjct: 74  ALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKN-GFPITIAALAFTFNLLNGYIQ 133

Query: 150 TRSVSHYV-KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVRLKRSG-GGYKVPTGGW 209
            R VSHY   YE   WFWWRF  G VVF+ GM +NI SD  LVRLK+   GGY +P GGW
Sbjct: 134 ARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGW 193

Query: 210 FELVSCPNYLGEIMEWVGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPR 269
           FELVSCPNY GE +EW+GWAVM  SWAG+GFFLYTC+NL PRARA+H WY+ KF E+YP+
Sbjct: 194 FELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPK 253

Query: 270 NRKAVIPFLY 278
            RKAVIPF+Y
Sbjct: 254 TRKAVIPFVY 262

[Close]

BLAST of PI0028471 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 80.1 bits (196), Expect = 3.1e-15
Identity = 39/108 (36.11%), Postives = 70/108 (64.81%), Query Frame = 0


Query: 172 GGVVFVMGMAVNIWSDGVLVRLKRSGG--GYKVPTGGWFELVSCPNYLGEIMEWVGWAVM 231
           G V+FV+G+  N++   +L +L++  G   YK+P GG F+++ CP+YL EI+ +  + ++
Sbjct: 162 GVVMFVVGIVGNLYHHVLLAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLI 221

Query: 232 CGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 278
             +     F + T   L+ R+ A  TWYL+KF +D+P++ KA+IPF++
Sbjct: 222 SQTIYSFSFAMGTMLYLIGRSYATRTWYLSKF-DDFPKHIKALIPFVF 268

[Close]

BLAST of PI0028471 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.4e-12
Identity = 41/114 (35.96%), Postives = 57/114 (50.00%), Query Frame = 0


Query: 172 GGVVFVMGMAVNIWSDGVLVRLKR---SGGGYKVPTGGWFELVSCPNYLGEIMEWVGWAV 231
           GG +F+ G         +L  L+        Y +P G WFE+VSCP++L EI+ ++G  +
Sbjct: 211 GGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYLGLLI 270

Query: 232 MCGS-----WAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 278
             G      W   GF     ANL   A   H WYL KF E+YP +R A+ P +Y
Sbjct: 271 SSGGTDISIWLLFGF---VAANLTYAAGETHRWYLQKF-ENYPASRHAIFPHVY 320

[Close]

BLAST of PI0028471 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 69.3 bits (168), Expect = 5.4e-12
Identity = 48/145 (33.10%), Postives = 67/145 (46.21%), Query Frame = 0


Query: 141 FNLLNSYLQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVRLKRS---G 200
           FNLL+      S+S  +K     W       GG +F+ G         +L  L+ +    
Sbjct: 215 FNLLS------SISPLMKLGSLQWI------GGAIFLWGWIHQRRCHAILGSLRENPSQA 274

Query: 201 GGYKVPTGGWFELVSCPNYLGEIMEWVGWAVMCGS-----WAGLGFFLYTCANLVPRARA 260
             Y +P G WF +VS P++L EI+ + G  +  G      W   GF     ANL   A  
Sbjct: 275 KEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDITIWLLFGF---VAANLTYAAGE 334

Query: 261 NHTWYLNKFGEDYPRNRKAVIPFLY 278
            H WYL KF E+YP NR A+ P++Y
Sbjct: 335 THRWYLRKF-ENYPANRHAIFPYVY 343

[Close]

BLAST of PI0028471 vs. TAIR 10
Match: AT2G16530.2 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 69.3 bits (168), Expect = 5.4e-12
Identity = 48/145 (33.10%), Postives = 67/145 (46.21%), Query Frame = 0


Query: 141 FNLLNSYLQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVRLKRS---G 200
           FNLL+      S+S  +K     W       GG +F+ G         +L  L+ +    
Sbjct: 214 FNLLS------SISPLMKLGSLQWI------GGAIFLWGWIHQRRCHAILGSLRENPSQA 273

Query: 201 GGYKVPTGGWFELVSCPNYLGEIMEWVGWAVMCGS-----WAGLGFFLYTCANLVPRARA 260
             Y +P G WF +VS P++L EI+ + G  +  G      W   GF     ANL   A  
Sbjct: 274 KEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDITIWLLFGF---VAANLTYAAGE 333

Query: 261 NHTWYLNKFGEDYPRNRKAVIPFLY 278
            H WYL KF E+YP NR A+ P++Y
Sbjct: 334 THRWYLRKF-ENYPANRHAIFPYVY 342

The following BLAST results are available for this feature:

BLAST of PI0028471 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2QDF6	5.9e-96	68.00	Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1	[more]
Q38944	5.5e-94	64.40	Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana OX=3702 GN=DET2 PE=1 SV=2	[more]
Q5K2N1	1.7e-82	60.42	Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum OX=4081 GN=DET2 PE=1 SV=1	[more]
Q68FF9	2.0e-35	38.33	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 ...	[more]
P24008	2.0e-35	37.04	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 ...	[more]

BLAST of PI0028471 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B6E3	5.3e-156	91.41	Steroid 5-alpha-reductase DET2 OS=Cucumis melo OX=3656 GN=LOC103486328 PE=3 SV=1	[more]
A0A0A0LAB3	1.0e-154	90.72	Steroid 5-alpha-reductase DET2 OS=Cucumis sativus OX=3659 GN=Csa_3G732550 PE=3 S...	[more]
A0A5D3DNY2	6.1e-144	96.39	Steroid 5-alpha-reductase DET2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A6J1J7F2	2.9e-130	87.55	Steroid 5-alpha-reductase DET2 OS=Cucurbita maxima OX=3661 GN=LOC111481932 PE=3 ...	[more]
A0A6J1F1U8	1.9e-129	87.15	Steroid 5-alpha-reductase DET2 OS=Cucurbita moschata OX=3662 GN=LOC111441400 PE=...	[more]

BLAST of PI0028471 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008442471.1	1.1e-155	91.41	PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis melo]	[more]
XP_004137737.1	2.1e-154	90.72	steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN58798.1 hypothetical protei...	[more]
KAA0044147.1	1.3e-143	96.39	steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa] >TYK24990.1 steroid 5-...	[more]
XP_038905208.1	1.8e-134	91.16	LOW QUALITY PROTEIN: steroid 5-alpha-reductase DET2 [Benincasa hispida]	[more]
XP_023527474.1	1.9e-131	88.76	steroid 5-alpha-reductase DET2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of PI0028471 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38050.1	3.9e-95	64.40	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT5G16010.1	3.1e-15	36.11	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	1.4e-12	35.96	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	5.4e-12	33.10	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.2	5.4e-12	33.10	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 130..277 e-value: 6.1E-49 score: 165.8
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 172..251 score: 27.063545
IPR016636	3-oxo-5-alpha-steroid 4-dehydrogenase	PIRSF	PIRSF015596	5_alpha-SR2	coord: 19..277 e-value: 8.8E-85 score: 282.0
None	No IPR available	GENE3D	1.20.120.1630		coord: 96..276 e-value: 4.1E-13 score: 51.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 7..22
None	No IPR available	PANTHER	PTHR10556:SF53	STEROID 5-ALPHA-REDUCTASE DET2	coord: 36..277
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 36..277

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0028471.1	PI0028471.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0008202	steroid metabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity
molecular_function	GO:0047751	cholestenone 5-alpha-reductase activity
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors