PI0028137 (gene) Melon (PI 482460) v1

Overview
NamePI0028137
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionacidic mammalian chitinase-like
Locationchr05: 1661364 .. 1662449 (-)
RNA-Seq ExpressionPI0028137
SyntenyPI0028137
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATATTGTTGCACCAGCAGCTCCCATCATAGATGTTGTAAAAAGTGGCTATTGGCTAGCAAGCACTGCCCCTATACTTCCTCCAGCTAATATCGATGCTACGCTTTTCACTCATCTCTTTGTTGGCTATGCTTATGTTGACTCAACGACTTATAAAGTTACCATAAATCCTTCACAAGATATCTTGATCAATGAGTTTTCTTCAATTGTGAGAATACGTAATCGGGACATCAAAACTTTGTTGTCCATTCAAGCAGGGAGTGAGGTATTTGCTGATATGGCAAAAGATGAAACAAATCGTGATGTGTTTATCAAATCCTCCATTGAAGTAGCAAGAGCAGGTTTGTATGATGGCCTAGATCTTCAATGGTTATACCCATCTTCTACTGCTGAAATGAACAACTTTGAAACTCTTATTGGTTCATGGCGCAAAGCTGTTGTGCAAGATGCTATCAATCTTAATCTTCCAGAGTTGTTGCTTGTTGCTTCAGTGTCTAACTTACCATATCTATATCCCGCCCAAAATATACAATATCCAATTCAAGCAATCAAACAAAATTTAAGTTGGGTGAATGTGATTTCTTATGATCTTTACACCCCAACTTCCTCAAATGATGCTACAGGACCATCCTCAGCATTCAACAATCCTAAAGCCAATGCCTTGTCTGCAAATTTTGGTATCCAGTCATGGACCCAAGCCAAGTTGCCTAGTAACAAAATTGTATTTGGAATTTCTTTTCATGGTTGGGCTTGGAAGCTTTCTGACTCTAATCAACATGATGTGTTTTCCGAAGCGGATGGTGCAGCTCAAGGAGGCAATATTTTGGTAGATGGAAAAATCTACTACTCTAATGTGATACAATTTATACACGATAAGCAAGCTGGTGTTGAGAATGACGACAAGAGGTTTCTAATTGCCTATACCTATTCAGACACTACGTGGATTACATATGAAAGTGAGTACTCAATTGCAGATAAGATTTCATTGGCAAAGTCTAAGAAGATTCTTGGTTACTTTACTTTCAACATTGCAGCAGATGATGATGTTAACACTCTTGCTAATGCTGCCCTTGCTAATTGGTGA

mRNA sequence

ATGGATATTGTTGCACCAGCAGCTCCCATCATAGATGTTGTAAAAAGTGGCTATTGGCTAGCAAGCACTGCCCCTATACTTCCTCCAGCTAATATCGATGCTACGCTTTTCACTCATCTCTTTGTTGGCTATGCTTATGTTGACTCAACGACTTATAAAGTTACCATAAATCCTTCACAAGATATCTTGATCAATGAGTTTTCTTCAATTGTGAGAATACGTAATCGGGACATCAAAACTTTGTTGTCCATTCAAGCAGGGAGTGAGGTATTTGCTGATATGGCAAAAGATGAAACAAATCGTGATGTGTTTATCAAATCCTCCATTGAAGTAGCAAGAGCAGGTTTGTATGATGGCCTAGATCTTCAATGGTTATACCCATCTTCTACTGCTGAAATGAACAACTTTGAAACTCTTATTGGTTCATGGCGCAAAGCTGTTGTGCAAGATGCTATCAATCTTAATCTTCCAGAGTTGTTGCTTGTTGCTTCAGTGTCTAACTTACCATATCTATATCCCGCCCAAAATATACAATATCCAATTCAAGCAATCAAACAAAATTTAAGTTGGGTGAATGTGATTTCTTATGATCTTTACACCCCAACTTCCTCAAATGATGCTACAGGACCATCCTCAGCATTCAACAATCCTAAAGCCAATGCCTTGTCTGCAAATTTTGGTATCCAGTCATGGACCCAAGCCAAGTTGCCTAGTAACAAAATTGTATTTGGAATTTCTTTTCATGGTTGGGCTTGGAAGCTTTCTGACTCTAATCAACATGATGTGTTTTCCGAAGCGGATGGTGCAGCTCAAGGAGGCAATATTTTGGTAGATGGAAAAATCTACTACTCTAATGTGATACAATTTATACACGATAAGCAAGCTGGTGTTGAGAATGACGACAAGAGGTTTCTAATTGCCTATACCTATTCAGACACTACGTGGATTACATATGAAAGTGAGTACTCAATTGCAGATAAGATTTCATTGGCAAAGTCTAAGAAGATTCTTGGTTACTTTACTTTCAACATTGCAGCAGATGATGATGTTAACACTCTTGCTAATGCTGCCCTTGCTAATTGGTGA

Coding sequence (CDS)

ATGGATATTGTTGCACCAGCAGCTCCCATCATAGATGTTGTAAAAAGTGGCTATTGGCTAGCAAGCACTGCCCCTATACTTCCTCCAGCTAATATCGATGCTACGCTTTTCACTCATCTCTTTGTTGGCTATGCTTATGTTGACTCAACGACTTATAAAGTTACCATAAATCCTTCACAAGATATCTTGATCAATGAGTTTTCTTCAATTGTGAGAATACGTAATCGGGACATCAAAACTTTGTTGTCCATTCAAGCAGGGAGTGAGGTATTTGCTGATATGGCAAAAGATGAAACAAATCGTGATGTGTTTATCAAATCCTCCATTGAAGTAGCAAGAGCAGGTTTGTATGATGGCCTAGATCTTCAATGGTTATACCCATCTTCTACTGCTGAAATGAACAACTTTGAAACTCTTATTGGTTCATGGCGCAAAGCTGTTGTGCAAGATGCTATCAATCTTAATCTTCCAGAGTTGTTGCTTGTTGCTTCAGTGTCTAACTTACCATATCTATATCCCGCCCAAAATATACAATATCCAATTCAAGCAATCAAACAAAATTTAAGTTGGGTGAATGTGATTTCTTATGATCTTTACACCCCAACTTCCTCAAATGATGCTACAGGACCATCCTCAGCATTCAACAATCCTAAAGCCAATGCCTTGTCTGCAAATTTTGGTATCCAGTCATGGACCCAAGCCAAGTTGCCTAGTAACAAAATTGTATTTGGAATTTCTTTTCATGGTTGGGCTTGGAAGCTTTCTGACTCTAATCAACATGATGTGTTTTCCGAAGCGGATGGTGCAGCTCAAGGAGGCAATATTTTGGTAGATGGAAAAATCTACTACTCTAATGTGATACAATTTATACACGATAAGCAAGCTGGTGTTGAGAATGACGACAAGAGGTTTCTAATTGCCTATACCTATTCAGACACTACGTGGATTACATATGAAAGTGAGTACTCAATTGCAGATAAGATTTCATTGGCAAAGTCTAAGAAGATTCTTGGTTACTTTACTTTCAACATTGCAGCAGATGATGATGTTAACACTCTTGCTAATGCTGCCCTTGCTAATTGGTGA

Protein sequence

MDIVAPAAPIIDVVKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDILINEFSSIVRIRNRDIKTLLSIQAGSEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQWLYPSSTAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQAIKQNLSWVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQAKLPSNKIVFGISFHGWAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDDKRFLIAYTYSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW
Homology
BLAST of PI0028137 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 3.3e-62
Identity = 139/352 (39.49%), Postives = 203/352 (57.67%), Query Frame = 0

Query: 13  VVKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDILINEFSSIVR 72
           VVK+ YW  ++    P  +ID++LFTHLF  +A ++S T +VT++ +     + F+  V+
Sbjct: 27  VVKASYWFPASE--FPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQ 86

Query: 73  IRNRDIKTLLSIQAG---SEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQWLYPSS 132
            RN  +KTLLSI  G      +A MA + T+R  FI SSI VAR+  + GLDL W YPSS
Sbjct: 87  RRNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSS 146

Query: 133 TAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQAIKQNLS 192
             EM NF TL+  WR AVV +A +   P LLL A+V    Y     ++ YP+ A+  +L 
Sbjct: 147 ATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVF---YSNNYYSVLYPVSAVASSLD 206

Query: 193 WVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQAKLPSNKIVFGISFHG 252
           WVN+++YD Y P  S   TGP +A  +P     S + G +SW QA LP+ K V G  ++G
Sbjct: 207 WVNLMAYDFYGPGWSR-VTGPPAALFDPSNAGPSGDAGTRSWIQAGLPAKKAVLGFPYYG 266

Query: 253 WAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDDKRFLIAYT 312
           +AW+L+++N H  ++   GAA    I  DG I Y  + +FI D  A     +   +  Y 
Sbjct: 267 YAWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDNGATTVY-NSTVVGDYC 326

Query: 313 YSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW 362
           Y+ T WI Y+   SI  K+  AK + +LGYF++++ ADD+ + L+ AA   W
Sbjct: 327 YAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAW 366

BLAST of PI0028137 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 4.9e-50
Identity = 120/352 (34.09%), Postives = 191/352 (54.26%), Query Frame = 0

Query: 14  VKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDILINEFSSIVRI 73
           VK  YWL +  P  PP  +D +LFTH+F  +   ++ TYK+ I+ SQ + +N F+   + 
Sbjct: 35  VKGIYWLEN--PFFPPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFTKTFKT 94

Query: 74  RNRDIKTLLSI---QAGSEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQWLYPSST 133
           ++    TL SI    + S + A +A D      FI S+I+VAR   +DG+DL W +P +T
Sbjct: 95  KSPPAATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWEFPKNT 154

Query: 134 AEMNNFETLIGSWRKAVVQDAINLNLPELLLVASV-SNLPYLYPAQNIQYPIQAIKQNLS 193
            EMN+   ++  WRKA+  +      P LLL A+V   + +    +   YP+ +I +NL 
Sbjct: 155 KEMNDLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSINENLD 214

Query: 194 WVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQAKLPSNKIVFGISFHG 253
           WVNV+SY+L  P S+   TG  S   +PK+N +S   G+ SW  + +   K+V G+  +G
Sbjct: 215 WVNVMSYELRGPRSNK--TGAPSGTFDPKSN-VSVVSGLLSWIHSGVVPEKLVMGMPLYG 274

Query: 254 WAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDDKRFLIAYT 313
            +WKL D N H + + + G+  G    V+G + Y  V+ F   K A VE D     + Y+
Sbjct: 275 KSWKLRDPNVHGIGAPSVGSGPG----VNGLMAYFQVLDFNRQKSAKVEYDVDTASV-YS 334

Query: 314 YSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW 362
           YS +TWI Y++ ++++ K+  A++ K+ GYF +    D     +A  A   W
Sbjct: 335 YSGSTWIGYDNPFTVSIKVGFAQALKLRGYFFWVAGLDTLDWKIATQASKAW 376

BLAST of PI0028137 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 193.0 bits (489), Expect = 6.0e-48
Identity = 122/357 (34.17%), Postives = 192/357 (53.78%), Query Frame = 0

Query: 14  VKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDILI----NEFSS 73
           VK  YW+ +  P+ PP++ID +LFTH+F  +   +  TYK+         +      F++
Sbjct: 37  VKGIYWIEN--PLFPPSSIDTSLFTHIFYAFVSPNKFTYKLEEEEEDSTTVATSLTTFTN 96

Query: 74  IVRIRNRDIKTLLSI---QAGSEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQWLY 133
             + +   I TLLSI    + S +FA +A D T R  FI S+I+VAR   +DG+D  W +
Sbjct: 97  TFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDGIDFDWEF 156

Query: 134 PSSTAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASV-SNLPYLYPAQNIQYPIQAIK 193
           P++T EMN+   L+  WR+A+  +  + + P LLL A+V   + +    +   YP+ +I 
Sbjct: 157 PTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRMYPVDSIN 216

Query: 194 QNLSWVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQAKLPSNKIVFGI 253
           +NL WVNV+SYDL    S ++ TG  S   + K+N +S   G+ SW +  +   KIV G+
Sbjct: 217 KNLDWVNVMSYDL--RGSGSNVTGAPSGMFDSKSN-VSVVSGLFSWIRGGVAPEKIVMGM 276

Query: 254 SFHGWAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVE-NDDKRF 313
             +G +WKL D N H + +   G   G    VDG + Y  V+ F  +KQ G +   DK  
Sbjct: 277 PLYGKSWKLQDPNVHGIGAPNVGPGPG----VDGGMAYFQVVDF--NKQMGAKVVYDKET 336

Query: 314 LIAYTYSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW 362
              Y+YS +TWI Y+  ++++ K+  A++ K+ GYF F  A  D  +   + A   W
Sbjct: 337 GSVYSYSGSTWIGYDDPFTVSVKVGFAQALKLGGYF-FWAAGYDTSDWKVSTASKAW 381

BLAST of PI0028137 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 2.2e-42
Identity = 114/343 (33.24%), Postives = 182/343 (53.06%), Query Frame = 0

Query: 14  VKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDILINEFSSIVRI 73
           V+S YW A       P+ ID   FTH+ + +   +  ++K+ I  S       F   +R 
Sbjct: 40  VRSAYWPAGDD--FSPSLIDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKALRH 99

Query: 74  RNRDIKTLLSIQAG---SEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQWLYPSST 133
           R+  +KTLLSI  G   S +F+++A  + NR++FI S+IEVAR   +DG+DL W +P + 
Sbjct: 100 RSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPETQ 159

Query: 134 AEMNNFETLIGSWRKAVVQDAINLNLPELLLVASV---SNLPYLYPAQNIQYPIQAIKQN 193
            +M N   L   W  A+  +A     P LLL ++V   S +  +       YP QAI + 
Sbjct: 160 QDMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAVYYNSTVRLIGKHGPRSYPTQAINKY 219

Query: 194 LSWVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQAKLPSNKIVFGISF 253
           L W + + +D +    +N  T  ++A  + K+  +S NFG+ SW ++ +   K+V G++ 
Sbjct: 220 LDWASPMCFDYHGTWDNN--TDFNAALYDSKSE-ISTNFGLHSWIKSGVRPEKLVMGLAL 279

Query: 254 HGWAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVE-NDDKRFLI 313
           +G AW+L D N + V +EA G A       DG + Y+ +++F  +KQ+G     DK  + 
Sbjct: 280 YGRAWELKDPNVNGVGAEAVGPATD----TDGSMNYNEILKF--NKQSGANVVYDKVAIS 339

Query: 314 AYTYSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDD 350
            Y+Y+ TTWI Y+   SI  K+  AKS  + GYF + +  D D
Sbjct: 340 FYSYAGTTWIGYDDGPSITTKVRFAKSLGLKGYFFWALGKDKD 371

BLAST of PI0028137 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 171.4 bits (433), Expect = 1.9e-41
Identity = 116/353 (32.86%), Postives = 186/353 (52.69%), Query Frame = 0

Query: 14  VKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDILINEFSSIVRI 73
           V++ YW A +      ++ID   FTH++  +      ++ + I  S      ++  I  I
Sbjct: 55  VRAAYWPAWSD--FSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAPKYDGIHNI 114

Query: 74  RNRDIKTLLSIQAG---SEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQWLYPSST 133
           R R + TLLSI  G   + +F++MA  + NR  FI S+I VAR   ++GLDL W +P   
Sbjct: 115 RPR-VTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWPGDE 174

Query: 134 AEMNNFETLIGSWRKAVVQDAINLNLPELLLVASV--SNLPYLYPAQNIQYPIQAIKQNL 193
            +M+N   L+  W KA+V +A       LLL ++V  ++   L       YP++AI++ L
Sbjct: 175 KDMSNLALLLKEWYKALVVEANTSRKSRLLLTSAVYFNSTISLIGNGPRSYPVRAIRKYL 234

Query: 194 SWVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQAKLPSNKIVFGISFH 253
            W + + +D Y    +N+ TG ++A  +P +N +S  +GI SW  + +P+ K+V G+  +
Sbjct: 235 DWASPMCFD-YNGAWANE-TGFNAALYDPNSN-ISTKYGIGSWIGSGVPAEKLVMGLPLY 294

Query: 254 GWAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDDKRFLIAY 313
           G AW+L D N H V ++A G A    +  DG + Y  ++ F  D  A V  D+   +  Y
Sbjct: 295 GRAWELKDPNDHGVGAKAVGPA----VDTDGSMDYDEILVFNKDTGAKVVYDEVA-VSFY 354

Query: 314 TYSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW 362
           +YS TTWI Y+   SI  K+  A+S  + GYF + I  D D  T++  A   W
Sbjct: 355 SYSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKDKDW-TISKQASNAW 395

BLAST of PI0028137 vs. ExPASy TrEMBL
Match: A0A6J1G2P4 (acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE=4 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.7e-128
Identity = 238/360 (66.11%), Postives = 286/360 (79.44%), Query Frame = 0

Query: 4   VAPAAPIIDVVKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDIL 63
           +A  A + + V +GYWLAST   LP ANIDATLFTHLF GYA ++S TY+VT++PSQ IL
Sbjct: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLIL 60

Query: 64  INEFSSIVRIRNRDIKTLLSIQAGSEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQ 123
           I++FSS V++ N ++KTLLSI A + VFA MA D TNRD FIKSSI  AR G +DGLDLQ
Sbjct: 61  IDQFSSTVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120

Query: 124 WLYPSSTAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQA 183
           WLYPSS  +M+NF+TLI SW +AVV DA +L+LP+LLLVA+VSNLPYL    NIQYP  A
Sbjct: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYL--QSNIQYPADA 180

Query: 184 IKQNLSWVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQA--KLPSNKI 243
           I Q L WVN++SYD YTP SS  ATGPSSAF+NPKANALSANFGI+SW Q+   LP  KI
Sbjct: 181 INQKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKI 240

Query: 244 VFGISFHGWAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDD 303
           VFGI FHGWAWKL +S+QH +F++ADGAA G  I   G+I+YS+V +FI D QAGVE+ D
Sbjct: 241 VFGIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKD 300

Query: 304 KRFLIAYTYSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW 362
           K +L+AY Y +TTWITYESEY+IADKI+LAK+ K+LGYF FNIAADD  NTLANAA +NW
Sbjct: 301 KSYLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of PI0028137 vs. ExPASy TrEMBL
Match: A0A6J1KFT3 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 1.5e-126
Identity = 234/360 (65.00%), Postives = 284/360 (78.89%), Query Frame = 0

Query: 4   VAPAAPIIDVVKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDIL 63
           +A  A + + V +GYWLAST   LP ANIDATLFTHLF GYA ++S TY+VT++PSQ IL
Sbjct: 1   MAEFANVTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLIL 60

Query: 64  INEFSSIVRIRNRDIKTLLSIQAGSEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQ 123
           I++FSS V++ N  +KTLLSI   + VFA MA D TNRD FIKSSI  AR G +DGLDLQ
Sbjct: 61  IDQFSSTVKLSNPGVKTLLSIAGDAAVFAAMAVDPTNRDAFIKSSIAAARGGGFDGLDLQ 120

Query: 124 WLYPSSTAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQA 183
           WLYPSS  +M NF+TLI SW +AVV DA +++LP+LLLVA+VSNLPYL    NIQYP  A
Sbjct: 121 WLYPSSQDQMANFKTLIQSWHQAVVDDATSVSLPQLLLVATVSNLPYL--QNNIQYPADA 180

Query: 184 IKQNLSWVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQA--KLPSNKI 243
           I QNL WVN++SYD YTP S  DATGPSSAF+NPKANALSANFGI+SW Q+   L   KI
Sbjct: 181 ITQNLDWVNLLSYDFYTPDSFRDATGPSSAFHNPKANALSANFGIRSWAQSLNNLSHKKI 240

Query: 244 VFGISFHGWAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDD 303
           VFGI FHGWAWKL++S+QH +F++ADGAA G  I   G+I+YS+V +FI D QAGVE+ D
Sbjct: 241 VFGIPFHGWAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKD 300

Query: 304 KRFLIAYTYSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW 362
           K +L+AY Y +TTWIT+ESEY++ADKI+LAK+ K+LGYF FNIAADD  NTLANAA +NW
Sbjct: 301 KSYLVAYAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of PI0028137 vs. ExPASy TrEMBL
Match: A0A6J1KB82 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 7.4e-126
Identity = 233/360 (64.72%), Postives = 283/360 (78.61%), Query Frame = 0

Query: 4   VAPAAPIIDVVKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDIL 63
           +A  A + + V +GYWLAST   LP ANIDATLFTHLF GYA ++S TY+VT++PSQ IL
Sbjct: 1   MAEFANVTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLIL 60

Query: 64  INEFSSIVRIRNRDIKTLLSIQAGSEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQ 123
           I++FSS V++ N D+KTLLSI   + VFA MA D TNRD FIKSSI  AR G +DGLDLQ
Sbjct: 61  IDQFSSTVKLSNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120

Query: 124 WLYPSSTAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQA 183
           WLYPSS  +M NF+TLI SW +AVV DA +++LP+LLLVA+VSNLPYL    NIQYP  A
Sbjct: 121 WLYPSSQDQMANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYL--QNNIQYPADA 180

Query: 184 IKQNLSWVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQA--KLPSNKI 243
           I Q L WVN++SYD YTP S   ATGPSSAF+NPKANALSANFGI+SW Q+   L   KI
Sbjct: 181 ITQKLDWVNLLSYDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLSHKKI 240

Query: 244 VFGISFHGWAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDD 303
           VFGI FHGWAWKL++S+QH +F++ADGAA G  I   G+I+YS+V +FI D QAGVE+ D
Sbjct: 241 VFGIPFHGWAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKD 300

Query: 304 KRFLIAYTYSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW 362
           K +L+AY Y +TTWIT+ESEY++ADKI+LAK+ K+LGYF FNIAADD  NTLANAA +NW
Sbjct: 301 KSYLVAYAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of PI0028137 vs. ExPASy TrEMBL
Match: A0A6J1G4H5 (chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 7.4e-126
Identity = 236/360 (65.56%), Postives = 281/360 (78.06%), Query Frame = 0

Query: 4   VAPAAPIIDVVKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDIL 63
           +A  A +   V +GYWLAST   L  ANIDATLFTHLFVGYA +D  T++VT++PSQ IL
Sbjct: 1   MAKFANVTKFVNAGYWLASTNDRLLAANIDATLFTHLFVGYALLDPKTHQVTVDPSQLIL 60

Query: 64  INEFSSIVRIRNRDIKTLLSIQAGSEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQ 123
           IN+FSS V++ N D+KTLLSI A + VFA MA   TNRD FIKSSI  AR G +DGLDLQ
Sbjct: 61  INQFSSTVKLSNPDVKTLLSIAADAAVFAAMAASPTNRDDFIKSSIAAARGGGFDGLDLQ 120

Query: 124 WLYPSSTAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQA 183
           WLYPSS  EM NF+TLI SW +AVV DA  ++LP+LLLVA+VSNLPYL   +N++YP  A
Sbjct: 121 WLYPSSQYEMGNFKTLIHSWHQAVVDDATRVSLPQLLLVATVSNLPYL--QKNVEYPADA 180

Query: 184 IKQNLSWVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQA--KLPSNKI 243
           I Q L WVN++SYD YTP SS  ATGPSSAF+NPKANALSANFGI+SW Q+   LP  KI
Sbjct: 181 INQKLDWVNLLSYDFYTPDSSKAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKI 240

Query: 244 VFGISFHGWAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDD 303
           VFGI FHGWAWKL +S+QH  F++ADGAA G  I   G+I+YS+V +FI D QAGVE+ D
Sbjct: 241 VFGIPFHGWAWKLFNSHQHAPFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKD 300

Query: 304 KRFLIAYTYSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW 362
           K +L+AY Y +TTWITYESEY+IADKI+LAK+ K+LGYF FNIAADD  NTLANAA +NW
Sbjct: 301 KSYLVAYAYYETTWITYESEYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of PI0028137 vs. ExPASy TrEMBL
Match: A0A6J1G4J9 (acidic mammalian chitinase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450666 PE=4 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 9.7e-126
Identity = 233/352 (66.19%), Postives = 277/352 (78.69%), Query Frame = 0

Query: 12  DVVKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDILINEFSSIV 71
           + V +GYWLA T   L  ANIDATLFTHLFVGYA +D  T++VT++PSQ ILIN+FSS V
Sbjct: 3   EFVNAGYWLAGTDDTLLAANIDATLFTHLFVGYALLDPKTHQVTVDPSQLILINQFSSTV 62

Query: 72  RIRNRDIKTLLSIQAGSEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQWLYPSSTA 131
           ++ N D+KTLLSI A + VFA MA   TNRD FIKSSI  AR G +DGLDLQWLYPSS  
Sbjct: 63  KLSNPDVKTLLSIAADAAVFAAMAASPTNRDDFIKSSIAAARGGGFDGLDLQWLYPSSQY 122

Query: 132 EMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQAIKQNLSWV 191
           EM NF+TLI SW +AVV DA  ++LP+LLLVA+VSNLPYL   +N++YP  AI Q L WV
Sbjct: 123 EMGNFKTLIHSWHQAVVDDATRVSLPQLLLVATVSNLPYL--QKNVEYPADAINQKLDWV 182

Query: 192 NVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQA--KLPSNKIVFGISFHG 251
           N++SYD YTP SS  ATGPSSAF+NPKANALSANFGI+SW Q+   LP  KIVFGI FHG
Sbjct: 183 NLLSYDFYTPDSSKAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHG 242

Query: 252 WAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDDKRFLIAYT 311
           WAWKL +S+QH  F++ADGAA G  I   G+I+YS+V +FI D QAGVE+ DK +L+AY 
Sbjct: 243 WAWKLFNSHQHAPFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYA 302

Query: 312 YSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW 362
           Y +TTWITYESEY+IADKI+LAK+ K+LGYF FNIAADD  NTLANAA +NW
Sbjct: 303 YYETTWITYESEYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 352

BLAST of PI0028137 vs. NCBI nr
Match: XP_023521369.1 (acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 471.5 bits (1212), Expect = 6.6e-129
Identity = 239/356 (67.13%), Postives = 283/356 (79.49%), Query Frame = 0

Query: 8   APIIDVVKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDILINEF 67
           A + + V +GYWLAST   LP ANIDATLFTHLF GYA ++S TY+VTI+PSQ IL+N+F
Sbjct: 11  ANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILLNQF 70

Query: 68  SSIVRIRNRDIKTLLSIQAGSEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQWLYP 127
           S  VR+ N D+KTLLSI A + VFA MA D TNRD FIKSSI  AR G +DGLDLQWLYP
Sbjct: 71  SPTVRLSNPDVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 130

Query: 128 SSTAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQAIKQN 187
           SS  EM NF+TLI SW +AVV DA +L+LP+LLLVA+VSNLPYL    NIQYP  AI Q 
Sbjct: 131 SSQEEMGNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYL--QSNIQYPADAINQK 190

Query: 188 LSWVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQA--KLPSNKIVFGI 247
           L WVN++SYD YTP S+  ATGPSSAF+NPKANALSANFGI+SW Q+   LP  KIVFGI
Sbjct: 191 LDWVNLLSYDFYTPDSNRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGI 250

Query: 248 SFHGWAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDDKRFL 307
            FHGWAWKL +S+QH +F++ADGAA G  I   G+I+YS+V +FI D QAGVE+ DK +L
Sbjct: 251 PFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYL 310

Query: 308 IAYTYSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW 362
           +AYTY +TTWITYES+Y+IADKI+LAK+ K+LGYF FNIAADD  NTLANAA +NW
Sbjct: 311 VAYTYFETTWITYESDYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 364

BLAST of PI0028137 vs. NCBI nr
Match: XP_023545402.1 (acidic mammalian chitinase-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 470.3 bits (1209), Expect = 1.5e-128
Identity = 238/350 (68.00%), Postives = 280/350 (80.00%), Query Frame = 0

Query: 14  VKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDILINEFSSIVRI 73
           V +GYWLAST   LP ANIDATLFTHLF GYA ++S TY+VTI+PSQ IL+N+FS  VR+
Sbjct: 2   VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILLNQFSPTVRL 61

Query: 74  RNRDIKTLLSIQAGSEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQWLYPSSTAEM 133
            N D+KTLLSI A + VFA MA D TNRD FIKSSI  AR G +DGLDLQWLYPSS  EM
Sbjct: 62  SNPDVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQEEM 121

Query: 134 NNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQAIKQNLSWVNV 193
            NF+TLI SW +AVV DA +L+LP+LLLVA+VSNLPYL    NIQYP  AI Q L WVN+
Sbjct: 122 GNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYL--QSNIQYPADAINQKLDWVNL 181

Query: 194 ISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQA--KLPSNKIVFGISFHGWA 253
           +SYD YTP S+  ATGPSSAF+NPKANALSANFGI+SW Q+   LP  KIVFGI FHGWA
Sbjct: 182 LSYDFYTPDSNRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWA 241

Query: 254 WKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDDKRFLIAYTYS 313
           WKL +S+QH +F++ADGAA G  I   G+I+YS+V +FI D QAGVE+ DK +L+AYTY 
Sbjct: 242 WKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYTYF 301

Query: 314 DTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW 362
           +TTWITYES+Y+IADKI+LAK+ K+LGYF FNIAADD  NTLANAA +NW
Sbjct: 302 ETTWITYESDYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 349

BLAST of PI0028137 vs. NCBI nr
Match: XP_022946077.1 (acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mammalian chitinase-like [Cucurbita moschata])

HSP 1 Score: 468.4 bits (1204), Expect = 5.6e-128
Identity = 238/360 (66.11%), Postives = 286/360 (79.44%), Query Frame = 0

Query: 4   VAPAAPIIDVVKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDIL 63
           +A  A + + V +GYWLAST   LP ANIDATLFTHLF GYA ++S TY+VT++PSQ IL
Sbjct: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLIL 60

Query: 64  INEFSSIVRIRNRDIKTLLSIQAGSEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQ 123
           I++FSS V++ N ++KTLLSI A + VFA MA D TNRD FIKSSI  AR G +DGLDLQ
Sbjct: 61  IDQFSSTVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120

Query: 124 WLYPSSTAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQA 183
           WLYPSS  +M+NF+TLI SW +AVV DA +L+LP+LLLVA+VSNLPYL    NIQYP  A
Sbjct: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYL--QSNIQYPADA 180

Query: 184 IKQNLSWVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQA--KLPSNKI 243
           I Q L WVN++SYD YTP SS  ATGPSSAF+NPKANALSANFGI+SW Q+   LP  KI
Sbjct: 181 INQKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKI 240

Query: 244 VFGISFHGWAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDD 303
           VFGI FHGWAWKL +S+QH +F++ADGAA G  I   G+I+YS+V +FI D QAGVE+ D
Sbjct: 241 VFGIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKD 300

Query: 304 KRFLIAYTYSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW 362
           K +L+AY Y +TTWITYESEY+IADKI+LAK+ K+LGYF FNIAADD  NTLANAA +NW
Sbjct: 301 KSYLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of PI0028137 vs. NCBI nr
Match: KAG6599362.1 (Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1 Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 468.0 bits (1203), Expect = 7.3e-128
Identity = 238/360 (66.11%), Postives = 286/360 (79.44%), Query Frame = 0

Query: 4   VAPAAPIIDVVKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDIL 63
           +A  A + + V +GYWLAST   LP ANIDATLFTHLF GYA ++S TY+VTI+PSQ IL
Sbjct: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLIL 60

Query: 64  INEFSSIVRIRNRDIKTLLSIQAGSEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQ 123
           I++FSS V++ N ++KTLLSI A + +FA MA D TNRD FIKSSI  AR G +DGLDLQ
Sbjct: 61  IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120

Query: 124 WLYPSSTAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQA 183
           WLYPSS  +M+NF+TLI SW +AVV DA +L+LP+LLLVA+VSNLPYL    NIQYP  A
Sbjct: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYL--QSNIQYPADA 180

Query: 184 IKQNLSWVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQA--KLPSNKI 243
           I Q L WVN++SYD YTP SS  ATGPSSAF+NPKANALSANFGI+SW Q+   LP  KI
Sbjct: 181 INQKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKI 240

Query: 244 VFGISFHGWAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDD 303
           VFGI FHGWAWKL +S+QH +F++ADGAA G  I   G+I+YS+V +FI D QAGVE+ D
Sbjct: 241 VFGIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKD 300

Query: 304 KRFLIAYTYSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW 362
           K +L+AY Y +TTWITYESEY+IADKI+LAK+ K+LGYF FNIAADD  NTLANAA +NW
Sbjct: 301 KSYLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of PI0028137 vs. NCBI nr
Match: KAG7030350.1 (Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 466.1 bits (1198), Expect = 2.8e-127
Identity = 237/360 (65.83%), Postives = 285/360 (79.17%), Query Frame = 0

Query: 4   VAPAAPIIDVVKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDIL 63
           +A  A + + V +GYWLAST   LP ANIDATLFTHLF GYA ++S TY+VTI+PS  IL
Sbjct: 1   MAEFANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLIL 60

Query: 64  INEFSSIVRIRNRDIKTLLSIQAGSEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQ 123
           I++FSS V++ N ++KTLLSI A + +FA MA D TNRD FIKSSI  AR G +DGLDLQ
Sbjct: 61  IDQFSSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQ 120

Query: 124 WLYPSSTAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQA 183
           WLYPSS  +M+NF+TLI SW +AVV DA +L+LP+LLLVA+VSNLPYL    NIQYP  A
Sbjct: 121 WLYPSSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYL--QSNIQYPADA 180

Query: 184 IKQNLSWVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQA--KLPSNKI 243
           I Q L WVN++SYD YTP SS  ATGPSSAF+NPKANALSANFGI+SW Q+   LP  KI
Sbjct: 181 INQKLDWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKI 240

Query: 244 VFGISFHGWAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDD 303
           VFGI FHGWAWKL +S+QH +F++ADGAA G  I   G+I+YS+V +FI D QAGVE+ D
Sbjct: 241 VFGIPFHGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKD 300

Query: 304 KRFLIAYTYSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW 362
           K +L+AY Y +TTWITYESEY+IADKI+LAK+ K+LGYF FNIAADD  NTLANAA +NW
Sbjct: 301 KSYLVAYAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of PI0028137 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 243.0 bits (619), Expect = 3.6e-64
Identity = 140/339 (41.30%), Postives = 195/339 (57.52%), Query Frame = 0

Query: 13  VVKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDILINEFSSIVR 72
           +VK+ YW+       P  NID+T FTHLF  +A VDS+T++VTI+ +    ++ F+  V+
Sbjct: 13  IVKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAANSCQVSSFTHTVK 72

Query: 73  IRNRDIKTLLSI---QAGSEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQWLYPSS 132
            +N D++TLLSI    A   V A MA +  NR  FI SSI++AR   + GLDL W YPS+
Sbjct: 73  DKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAWEYPSN 132

Query: 133 TAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQAIKQNLS 192
             EM NF  L+  WR AVV+++   N   LLL A+V   P  Y     +YP+QAI  NL 
Sbjct: 133 DVEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPDYY---GEEYPVQAIADNLD 192

Query: 193 WVNVISYDLYTPTSSNDATGPSSAFNNPKANA-LSANFGIQSWTQAKLPSNKIVFGISFH 252
           +VN+++YD Y P  S   TGP +A  +P   A  S + G+  W +AKLP+ K V G S+ 
Sbjct: 193 FVNIMAYDFYGPGWS-PVTGPPAALFDPSNPAGRSGDSGLSKWLEAKLPAKKAVLGFSYC 252

Query: 253 GWAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDDKRFLIAY 312
           GWAW L D+  +   +  DGAA    I  DG I Y+ +  +I D  A   + D   +  Y
Sbjct: 253 GWAWTLEDAENNGYDAATDGAA----ISSDGSITYAKIRNYIIDNGAATFH-DPAVIGFY 312

Query: 313 TYSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAAD 348
            Y  TTWI Y+   SI  K+  AK K +LGYF++++ AD
Sbjct: 313 CYVGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSWHVGAD 342

BLAST of PI0028137 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 240.4 bits (612), Expect = 2.3e-63
Identity = 139/352 (39.49%), Postives = 203/352 (57.67%), Query Frame = 0

Query: 13  VVKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDILINEFSSIVR 72
           VVK+ YW  ++    P  +ID++LFTHLF  +A ++S T +VT++ +     + F+  V+
Sbjct: 27  VVKASYWFPASE--FPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQ 86

Query: 73  IRNRDIKTLLSIQAG---SEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQWLYPSS 132
            RN  +KTLLSI  G      +A MA + T+R  FI SSI VAR+  + GLDL W YPSS
Sbjct: 87  RRNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSS 146

Query: 133 TAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQAIKQNLS 192
             EM NF TL+  WR AVV +A +   P LLL A+V    Y     ++ YP+ A+  +L 
Sbjct: 147 ATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVF---YSNNYYSVLYPVSAVASSLD 206

Query: 193 WVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQAKLPSNKIVFGISFHG 252
           WVN+++YD Y P  S   TGP +A  +P     S + G +SW QA LP+ K V G  ++G
Sbjct: 207 WVNLMAYDFYGPGWSR-VTGPPAALFDPSNAGPSGDAGTRSWIQAGLPAKKAVLGFPYYG 266

Query: 253 WAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDDKRFLIAYT 312
           +AW+L+++N H  ++   GAA    I  DG I Y  + +FI D  A     +   +  Y 
Sbjct: 267 YAWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDNGATTVY-NSTVVGDYC 326

Query: 313 YSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALANW 362
           Y+ T WI Y+   SI  K+  AK + +LGYF++++ ADD+ + L+ AA   W
Sbjct: 327 YAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAW 366

BLAST of PI0028137 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 232.3 bits (591), Expect = 6.3e-61
Identity = 131/340 (38.53%), Postives = 198/340 (58.24%), Query Frame = 0

Query: 13  VVKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDILINEFSSIVR 72
           VVK+ YW A +    P A ID++LFTHLF  +A +++ TY+V ++       + F+  VR
Sbjct: 25  VVKATYWFAESES--PLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTFTQTVR 84

Query: 73  IRNRDIKTLLSIQAG---SEVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQWLYPSS 132
            RN  +KTLLSI      +  FA MA + T+R +FI SSI++AR+  + GLDL W YPS 
Sbjct: 85  RRNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWKYPSI 144

Query: 133 TAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQAIKQNLS 192
           T EM+NF  L+  WR AV  +A +   P LLL A+V    Y Y   ++ +P+ A+  +L 
Sbjct: 145 TTEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAVF---YSYSYYSVLHPVNAVADSLD 204

Query: 193 WVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQAKLPSNKIVFGISFHG 252
           WVN+++YD Y   SS     P+  + +P     S + G+++WTQA LP+ K V G   +G
Sbjct: 205 WVNLVAYDFYESGSSRVTCSPAPLY-DPITTGPSGDAGVRAWTQAGLPAKKAVLGFPLYG 264

Query: 253 WAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDDKRFLIAYT 312
           +AW L+D+  H+ ++ + G A    I  DG I Y  + +FI D +A +   +   +  Y 
Sbjct: 265 YAWCLTDAKNHNYYANSSGPA----ISPDGSIGYDQIRRFIVDNKATMVY-NSNLVQNYC 324

Query: 313 YSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDD 350
           Y+  TWI Y+   SI  K+  AK + +LGYF+++I ADD+
Sbjct: 325 YAKKTWIGYDDNQSIVMKVKYAKQRGLLGYFSWHIGADDN 353

BLAST of PI0028137 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 225.3 bits (573), Expect = 7.7e-59
Identity = 140/362 (38.67%), Postives = 200/362 (55.25%), Query Frame = 0

Query: 13  VVKSGYWL---ASTAPI-----LPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDILI 72
           +VK+ YW     S +P       P + ID+TLFTHLF  +A VDS+T++VTI+ +     
Sbjct: 13  IVKASYWFPDGKSQSPECLSQGTPSSFIDSTLFTHLFCAFADVDSSTHEVTISAANSYQF 72

Query: 73  NEFSSIVRIRNRDIKTLLSI---QAGSEVFADMAKDETNRDVFIKSSIEVARAGLYDGLD 132
           + F+  V+ +N D++TLLSI    A   V A MA +  NR  FI SSI++AR   + GLD
Sbjct: 73  SSFTETVKEKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLD 132

Query: 133 LQWLYPSSTAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPI 192
           L W YPS+  EM NF  L+  WR AVV+++   N   LLL A+V   P       ++YP+
Sbjct: 133 LAWEYPSNDVEMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYSPQY---DGVEYPV 192

Query: 193 QAIKQNLSWVNVISYDLYTPTSSNDATGPSSAFNNPKANA-LSANFGIQSW-TQAKLPSN 252
           +AI  NL +VN+++YD Y P  S     P++ F++P   A  S N G++ W  +AKLP  
Sbjct: 193 KAIADNLDFVNIMAYDFYGPGWSPVTGPPAALFHDPSNPAGRSGNSGLRKWLDEAKLPPK 252

Query: 253 KIVFGISFHGWAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVEN 312
           K V G  + GWAW L D+  +   +  DGAA    I  DG I Y+ +  +I D  A   +
Sbjct: 253 KAVLGFPYCGWAWTLEDAENNGYDAATDGAA----ISPDGSITYAKIRNYIVDNGAATFH 312

Query: 313 DDKRFLIAYTYSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDDVNTLANAALA 362
            D   +  Y Y   TWI Y+   SI  K+  AK   +LGYF++++ AD +   L+ AA  
Sbjct: 313 -DPAVIGFYCYVGNTWIGYDDNQSIVYKVKYAKFTGLLGYFSWHVGADYNCG-LSRAASI 365

BLAST of PI0028137 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 220.3 bits (560), Expect = 2.5e-57
Identity = 129/341 (37.83%), Postives = 196/341 (57.48%), Query Frame = 0

Query: 12  DVVKSGYWLASTAPILPPANIDATLFTHLFVGYAYVDSTTYKVTINPSQDILINEFSSIV 71
           DVVK+ YW  +T    P  +ID++LFTHLF  +A +++ +Y++TI        + F+  V
Sbjct: 5   DVVKASYWFPATD--FPATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAFTETV 64

Query: 72  RIRNRDIKTLLSIQAGS---EVFADMAKDETNRDVFIKSSIEVARAGLYDGLDLQWLYPS 131
           + RN  +KTLLSI  G+   + FA MA +  +R  FI+S+I VAR+  + GLDL W YP 
Sbjct: 65  QQRNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDWEYPR 124

Query: 132 STAEMNNFETLIGSWRKAVVQDAINLNLPELLLVASVSNLPYLYPAQNIQYPIQAIKQNL 191
           +  EM +F  L+  WR AV  ++ +     L+L A+V    Y    Q + YP+ AI  +L
Sbjct: 125 NEEEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAVY---YSSNYQGVPYPVLAISNSL 184

Query: 192 SWVNVISYDLYTPTSSNDATGPSSAFNNPKANALSANFGIQSWTQAKLPSNKIVFGISFH 251
            W+N+++YD Y P  S   TGP ++   P  +  S + G++ WT+A LP+ K V G  ++
Sbjct: 185 DWINLMAYDFYGPGWST-VTGPPASLYLP-TDGRSGDSGVRDWTEAGLPAKKAVLGFPYY 244

Query: 252 GWAWKLSDSNQHDVFSEADGAAQGGNILVDGKIYYSNVIQFIHDKQAGVENDDKRFLIAY 311
           GWAW L+D + +   +   G A    I  DG+I Y  +  +I D  A   +DD   +  Y
Sbjct: 245 GWAWTLADPDVNGYDANTTGPA----ISDDGEISYRQLQTWIVDNGATKVHDD-MMVGDY 304

Query: 312 TYSDTTWITYESEYSIADKISLAKSKKILGYFTFNIAADDD 350
            Y+ TTWI Y+SE SI  K+  AK K +LGYF++++  DD+
Sbjct: 305 CYAGTTWIGYDSEKSIVTKVIYAKQKGLLGYFSWHVGGDDN 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O818623.3e-6239.49Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1[more]
A0A072UR654.9e-5034.09Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1[more]
U5N4E36.0e-4834.17Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2[more]
A0A072VEP02.2e-4233.24Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1[more]
A0A1B1J8Z21.9e-4132.86Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1G2P42.7e-12866.11acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE... [more]
A0A6J1KFT31.5e-12665.00chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1[more]
A0A6J1KB827.4e-12664.72chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1[more]
A0A6J1G4H57.4e-12665.56chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 ... [more]
A0A6J1G4J99.7e-12666.19acidic mammalian chitinase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_023521369.16.6e-12967.13acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo][more]
XP_023545402.11.5e-12868.00acidic mammalian chitinase-like, partial [Cucurbita pepo subsp. pepo][more]
XP_022946077.15.6e-12866.11acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mamm... [more]
KAG6599362.17.3e-12866.11Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1... [more]
KAG7030350.12.8e-12765.83Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT4G19750.13.6e-6441.30Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19810.12.3e-6339.49Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19820.16.3e-6138.53Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19760.17.7e-5938.67Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19800.12.5e-5737.83Glycosyl hydrolase family protein with chitinase insertion domain [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011583Chitinase IISMARTSM006362g34coord: 13..347
e-value: 3.4E-57
score: 206.0
IPR001223Glycoside hydrolase family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 26..347
e-value: 4.8E-51
score: 174.3
IPR001223Glycoside hydrolase family 18, catalytic domainPROSITEPS51910GH18_2coord: 13..361
score: 44.962891
IPR029070Chitinase insertion domain superfamilyGENE3D3.10.50.10coord: 247..318
e-value: 7.3E-75
score: 254.3
IPR029070Chitinase insertion domain superfamilySUPERFAMILY54556Chitinase insertion domaincoord: 248..318
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 31..347
e-value: 7.3E-75
score: 254.3
NoneNo IPR availablePANTHERPTHR11177CHITINASEcoord: 11..355
NoneNo IPR availablePANTHERPTHR11177:SF351CHITINASE-LIKE PROTEIN-RELATEDcoord: 11..355
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 17..352

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0028137.1PI0028137.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0008061 chitin binding
molecular_function GO:0016787 hydrolase activity