PI0028107 (gene) Melon (PI 482460) v1

Overview


Name	PI0028107
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Plant protein 1589 of unknown function
Location	chr08: 16335964 .. 16337034 (+)
RNA-Seq Expression	PI0028107
Synteny	PI0028107

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACCCCCACAATATTTTCTCTCACTTTTCCCTCCACTTTCTCTCGTTTTCTCTCCATCCAAACAGAAATCATTTACACCACCATGGACGACGACCCCTCTGCTTCATACATCCACATGGTATTTTCTTTTTCTTATTTTTTTTCTTTTTAATTTTTATTTGTCGATTTATATAATGAACGGACGGATAATATGCGGGCGTATGATTGTTACGGTGTGTAGGTGCACCACCTGATTGAAAAGTGTATAATTTTCAACATGACTAAAGAAGAGTGCTTGGAAGCTCTCTCCAAACATGCAAACATCGAACCGGTCATCACTTCCACCGGTTCGTTTCTCTCTCACGTGACATGCACGGACTCCGAATTCTCTTTTATTTTTCATTCATTTTTTTTTTTTTTAATTTCCTTAGTGTGGAATGAATTGGAGAAGGAGAACAAGGAATTCTTCGAAGCGTATAAGAAGAAACGACGTGACGTTACTACGAAGGAGGAGAATTCATCCGACGCGACGGATTTCAATCTGCTGGGTTCGAGGGATTCGACGTCGTCTTTGAGGCAGTAGGTGAATAGACAACCGTCGGTGTAGGCTGGGTGGAAATTAAATAAATAAATAAATAAAATAAAATAAAGAAAAGAAATGTACTGACAGCGGCGAAGCGACGTACAACGTCGTTTTTAGGGTCGTAGTGTAGGTGGAATGGAACAGCAGCAACGGCGGCGTAGGGAGGGGCCGTGATGGGGTAATTGAGGGGCGTGAAGATATTTTGGGATACTATGGCTGACACGTGGCTACACGTGGCATCGTGCTCTCAACTTTTAAAATATGTATCGCTGTCGTTTGCTGATTTGGATTGTCGGGAGATGTAGGGATTTAAGTGGAATTTCCTCTCTCTTTTTTTTTTAAATTAGCCGTCCAAGCCAATTGGTAATGCCACCTGTTTTTTTGTTTTTCTTTCTTTCTAATTTAAAAAATGTAAAATTATAAGTAACGGTAATAATTTTAGTCTTCTTTTATGATGGATTAAATTTCTGAATTTAAATTTTTGGCAAACGGTAAAAATCAACTCAAAA

mRNA sequence

CACCCCCACAATATTTTCTCTCACTTTTCCCTCCACTTTCTCTCGTTTTCTCTCCATCCAAACAGAAATCATTTACACCACCATGGACGACGACCCCTCTGCTTCATACATCCACATGGTGCACCACCTGATTGAAAAGTGTATAATTTTCAACATGACTAAAGAAGAGTGCTTGGAAGCTCTCTCCAAACATGCAAACATCGAACCGGTCATCACTTCCACCGTGTGGAATGAATTGGAGAAGGAGAACAAGGAATTCTTCGAAGCGTATAAGAAGAAACGACGTGACGTTACTACGAAGGAGGAGAATTCATCCGACGCGACGGATTTCAATCTGCTGGGTTCGAGGGATTCGACGTCGTCTTTGAGGCAGTAGGTGAATAGACAACCGTCGGTGTAGGCTGGGTGGAAATTAAATAAATAAATAAATAAAATAAAATAAAGAAAAGAAATGTACTGACAGCGGCGAAGCGACGTACAACGTCGTTTTTAGGGTCGTAGTGTAGGTGGAATGGAACAGCAGCAACGGCGGCGTAGGGAGGGGCCGTGATGGGGTAATTGAGGGGCGTGAAGATATTTTGGGATACTATGGCTGACACGTGGCTACACGTGGCATCGTGCTCTCAACTTTTAAAATATGTATCGCTGTCGTTTGCTGATTTGGATTGTCGGGAGATGTAGGGATTTAAGTGGAATTTCCTCTCTCTTTTTTTTTTAAATTAGCCGTCCAAGCCAATTGGTAATGCCACCTGTTTTTTTGTTTTTCTTTCTTTCTAATTTAAAAAATGTAAAATTATAAGTAACGGTAATAATTTTAGTCTTCTTTTATGATGGATTAAATTTCTGAATTTAAATTTTTGGCAAACGGTAAAAATCAACTCAAAA

Coding sequence (CDS)

ATGGACGACGACCCCTCTGCTTCATACATCCACATGGTGCACCACCTGATTGAAAAGTGTATAATTTTCAACATGACTAAAGAAGAGTGCTTGGAAGCTCTCTCCAAACATGCAAACATCGAACCGGTCATCACTTCCACCGTGTGGAATGAATTGGAGAAGGAGAACAAGGAATTCTTCGAAGCGTATAAGAAGAAACGACGTGACGTTACTACGAAGGAGGAGAATTCATCCGACGCGACGGATTTCAATCTGCTGGGTTCGAGGGATTCGACGTCGTCTTTGAGGCAGTAG

Protein sequence

MDDDPSASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFFEAYKKKRRDVTTKEENSSDATDFNLLGSRDSTSSLRQ

Homology

[Close]

BLAST of PI0028107 vs. ExPASy TrEMBL
Match: A0A0A0KZG8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G293290 PE=4 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 4.0e-42
Identity = 89/95 (93.68%), Postives = 93/95 (97.89%), Query Frame = 0


Query: 1  MDDDPSASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFF 60
          MDDDPSASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFF
Sbjct: 1  MDDDPSASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFF 60

Query: 61 EAYKKKRRDVTTKEENSSDATDFNLLGSRDSTSSL 96
          EAYKKKRRDVTTK+ +SS+ TDFNLLGSRD+TSSL
Sbjct: 61 EAYKKKRRDVTTKKGSSSETTDFNLLGSRDTTSSL 95

[Close]

BLAST of PI0028107 vs. ExPASy TrEMBL
Match: A0A1S3BI57 (uncharacterized protein LOC103489916 OS=Cucumis melo OX=3656 GN=LOC103489916 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 5.0e-37
Identity = 82/86 (95.35%), Postives = 84/86 (97.67%), Query Frame = 0


Query: 12 MVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFFEAYKKKRRDVT 71
          MVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFFEAYKKKRRDVT
Sbjct: 1  MVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFFEAYKKKRRDVT 60

Query: 72 TKEENSSDATDFNLLGSRDSTSSLRQ 98
          TKE NSSD TDFNLLGSRD+TSSLR+
Sbjct: 61 TKEGNSSDTTDFNLLGSRDTTSSLRR 86

[Close]

BLAST of PI0028107 vs. ExPASy TrEMBL
Match: A0A6J1IHY2 (uncharacterized protein LOC111477620 OS=Cucurbita maxima OX=3661 GN=LOC111477620 PE=4 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.1e-35
Identity = 78/95 (82.11%), Postives = 87/95 (91.58%), Query Frame = 0


Query: 1  MDDDPSASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFF 60
          M+DDPSASYIHMVHHLIE+CI+FNM KEEC+EALSKHANI+PVITSTVWNELEKENKEFF
Sbjct: 1  MEDDPSASYIHMVHHLIEQCIVFNMNKEECMEALSKHANIKPVITSTVWNELEKENKEFF 60

Query: 61 EAYKKKRRDVTTK--EENSSDATDFNLLGSRDSTS 94
          EAYKKKRRDVTT+  +  SSD +DFNLL S D+TS
Sbjct: 61 EAYKKKRRDVTTRAADGTSSDTSDFNLLDSGDTTS 95

[Close]

BLAST of PI0028107 vs. ExPASy TrEMBL
Match: A0A6J1IAZ7 (uncharacterized protein LOC111471258 OS=Cucurbita maxima OX=3661 GN=LOC111471258 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 8.9e-34
Identity = 76/96 (79.17%), Postives = 86/96 (89.58%), Query Frame = 0


Query: 1  MDDDPSASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFF 60
          M+DDPSASYIHMVHHLIE CI+FNM+KEEC+EALSKHANIEPVITSTVWNELEKENKEFF
Sbjct: 1  MEDDPSASYIHMVHHLIEICILFNMSKEECMEALSKHANIEPVITSTVWNELEKENKEFF 60

Query: 61 EAYKKKRRDVTTKE---ENSSDATDFNLLGSRDSTS 94
          +AY+KKRRDVTT++    +SSD   FNLLG  D+TS
Sbjct: 61 QAYEKKRRDVTTRKLEGNSSSDTLGFNLLGLGDTTS 96

[Close]

BLAST of PI0028107 vs. ExPASy TrEMBL
Match: A0A6J1HDK1 (uncharacterized protein LOC111463185 OS=Cucurbita moschata OX=3662 GN=LOC111463185 PE=4 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.3e-32
Identity = 74/95 (77.89%), Postives = 85/95 (89.47%), Query Frame = 0


Query: 1  MDDDPSASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFF 60
          M+DDPSASYIHMVHHLIE  I+FNM+KEEC+EALSKHANIEPV+TSTVWNELEKENKEFF
Sbjct: 1  MEDDPSASYIHMVHHLIEISILFNMSKEECMEALSKHANIEPVVTSTVWNELEKENKEFF 60

Query: 61 EAYKKKRRDVTTKE---ENSSDATDFNLLGSRDST 93
          EAY+KKRRDVTT++    +SSD + FNLLG  D+T
Sbjct: 61 EAYEKKRRDVTTRKLEGNSSSDTSAFNLLGLGDTT 95

[Close]

BLAST of PI0028107 vs. NCBI nr
Match: XP_004142092.1 (uncharacterized protein LOC101219487 [Cucumis sativus] >KGN54219.1 hypothetical protein Csa_018164 [Cucumis sativus])

HSP 1 Score: 180.3 bits (456), Expect = 8.2e-42
Identity = 89/95 (93.68%), Postives = 93/95 (97.89%), Query Frame = 0


Query: 1  MDDDPSASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFF 60
          MDDDPSASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFF
Sbjct: 1  MDDDPSASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFF 60

Query: 61 EAYKKKRRDVTTKEENSSDATDFNLLGSRDSTSSL 96
          EAYKKKRRDVTTK+ +SS+ TDFNLLGSRD+TSSL
Sbjct: 61 EAYKKKRRDVTTKKGSSSETTDFNLLGSRDTTSSL 95

[Close]

BLAST of PI0028107 vs. NCBI nr
Match: XP_038883060.1 (uncharacterized protein LOC120074114 [Benincasa hispida])

HSP 1 Score: 174.5 bits (441), Expect = 4.5e-40
Identity = 88/97 (90.72%), Postives = 92/97 (94.85%), Query Frame = 0


Query: 1  MDDDPSASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFF 60
          MDDDPSASYIHMVHHLIEKCIIFNMTKEEC+EALSKHANI+PVITSTVWNELEKENKEFF
Sbjct: 1  MDDDPSASYIHMVHHLIEKCIIFNMTKEECMEALSKHANIKPVITSTVWNELEKENKEFF 60

Query: 61 EAYKKKRRDVTTK--EENSSDATDFNLLGSRDSTSSL 96
          EAYKKKRRDVT K  E NSSD +DFNLLGSRD+TSSL
Sbjct: 61 EAYKKKRRDVTVKVSEGNSSDTSDFNLLGSRDTTSSL 97

[Close]

BLAST of PI0028107 vs. NCBI nr
Match: XP_008447483.1 (PREDICTED: uncharacterized protein LOC103489916 [Cucumis melo])

HSP 1 Score: 163.3 bits (412), Expect = 1.0e-36
Identity = 82/86 (95.35%), Postives = 84/86 (97.67%), Query Frame = 0


Query: 12 MVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFFEAYKKKRRDVT 71
          MVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFFEAYKKKRRDVT
Sbjct: 1  MVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFFEAYKKKRRDVT 60

Query: 72 TKEENSSDATDFNLLGSRDSTSSLRQ 98
          TKE NSSD TDFNLLGSRD+TSSLR+
Sbjct: 61 TKEGNSSDTTDFNLLGSRDTTSSLRR 86

[Close]

BLAST of PI0028107 vs. NCBI nr
Match: XP_023544368.1 (uncharacterized protein LOC111803972 [Cucurbita pepo subsp. pepo] >KAG7033856.1 hypothetical protein SDJN02_03581 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 158.7 bits (400), Expect = 2.6e-35
Identity = 79/95 (83.16%), Postives = 88/95 (92.63%), Query Frame = 0


Query: 1  MDDDPSASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFF 60
          M+DDPSASYIHMVHHLIE+CIIFNM KEEC+EALSKHANI+PVITSTVWNELEKENKEFF
Sbjct: 1  MEDDPSASYIHMVHHLIEQCIIFNMNKEECMEALSKHANIKPVITSTVWNELEKENKEFF 60

Query: 61 EAYKKKRRDVTTK--EENSSDATDFNLLGSRDSTS 94
          EAYKKKRRDVTT+  + NSSD +DFNLL S ++TS
Sbjct: 61 EAYKKKRRDVTTRAADGNSSDTSDFNLLDSGNTTS 95

[Close]

BLAST of PI0028107 vs. NCBI nr
Match: XP_022977252.1 (uncharacterized protein LOC111477620 [Cucurbita maxima])

HSP 1 Score: 157.9 bits (398), Expect = 4.4e-35
Identity = 78/95 (82.11%), Postives = 87/95 (91.58%), Query Frame = 0


Query: 1  MDDDPSASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFF 60
          M+DDPSASYIHMVHHLIE+CI+FNM KEEC+EALSKHANI+PVITSTVWNELEKENKEFF
Sbjct: 1  MEDDPSASYIHMVHHLIEQCIVFNMNKEECMEALSKHANIKPVITSTVWNELEKENKEFF 60

Query: 61 EAYKKKRRDVTTK--EENSSDATDFNLLGSRDSTS 94
          EAYKKKRRDVTT+  +  SSD +DFNLL S D+TS
Sbjct: 61 EAYKKKRRDVTTRAADGTSSDTSDFNLLDSGDTTS 95

[Close]

BLAST of PI0028107 vs. TAIR 10
Match: AT5G02580.1 (Plant protein 1589 of unknown function )

HSP 1 Score: 111.7 bits (278), Expect = 3.3e-25
Identity = 50/76 (65.79%), Postives = 65/76 (85.53%), Query Frame = 0


Query: 7  ASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFFEAYKKK 66
          ASYIH+VHHLIE+CI+FNM KEEC++AL KHANI+P+ITSTVW EL KENKEFFEAY+++
Sbjct: 9  ASYIHLVHHLIEECIVFNMGKEECMDALFKHANIKPIITSTVWKELAKENKEFFEAYERR 68

Query: 67 RRDVTTKEENSSDATD 83
          R ++ T++E +    D
Sbjct: 69 REEIPTEKETARRIRD 84

[Close]

BLAST of PI0028107 vs. TAIR 10
Match: AT3G55240.1 (Plant protein 1589 of unknown function )

HSP 1 Score: 109.4 bits (272), Expect = 1.7e-24
Identity = 57/94 (60.64%), Postives = 72/94 (76.60%), Query Frame = 0


Query: 1  MDDDPSASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFF 60
          M D  SASYIHMV H+IEKC+IF+M+KEEC+EALSKHANI PVITSTVW ELEKENKEFF
Sbjct: 1  MADSSSASYIHMVQHMIEKCLIFHMSKEECVEALSKHANITPVITSTVWKELEKENKEFF 60

Query: 61 EAYKKKRRDVTTKEENSSDATDFNLLGSRDSTSS 95
          +AY++++    +K+E  S+     ++    S SS
Sbjct: 61 KAYEERQ----SKQEQMSEEETNQMIQKIISDSS 90

[Close]

BLAST of PI0028107 vs. TAIR 10
Match: AT3G28990.1 (Plant protein 1589 of unknown function )

HSP 1 Score: 93.6 bits (231), Expect = 9.4e-20
Identity = 45/76 (59.21%), Postives = 59/76 (77.63%), Query Frame = 0


Query: 7  ASYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFFEAYKKK 66
          A YIH+V H+IE C+ FNM+KEEC+EALS++ANI P+ITSTVW EL KENK+FFE Y++K
Sbjct: 7  AVYIHLVQHMIETCLTFNMSKEECMEALSENANINPIITSTVWKELVKENKDFFETYEQK 66

Query: 67 RRDVTTKEENSSDATD 83
             +  KE  S + T+
Sbjct: 67 ---LMKKESMSEEETN 79

[Close]

BLAST of PI0028107 vs. TAIR 10
Match: AT1G10657.2 (Plant protein 1589 of unknown function )

HSP 1 Score: 77.8 bits (190), Expect = 5.4e-15
Identity = 32/56 (57.14%), Postives = 45/56 (80.36%), Query Frame = 0


Query: 8  SYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFFEAY 64
          SYI MV H+IE+CI+  MT++EC++AL  HA+I P++T TVW  L++ENK+FFE Y
Sbjct: 22 SYIRMVQHMIERCILLRMTRDECVKALDHHASILPLVTLTVWRGLQRENKDFFETY 77

[Close]

BLAST of PI0028107 vs. TAIR 10
Match: AT1G10657.3 (Plant protein 1589 of unknown function )

HSP 1 Score: 77.8 bits (190), Expect = 5.4e-15
Identity = 32/56 (57.14%), Postives = 45/56 (80.36%), Query Frame = 0


Query: 8  SYIHMVHHLIEKCIIFNMTKEECLEALSKHANIEPVITSTVWNELEKENKEFFEAY 64
          SYI MV H+IE+CI+  MT++EC++AL  HA+I P++T TVW  L++ENK+FFE Y
Sbjct: 22 SYIRMVQHMIERCILLRMTRDECVKALDHHASILPLVTLTVWRGLQRENKDFFETY 77

The following BLAST results are available for this feature:

BLAST of PI0028107 vs.


Match Name	E-value	Identity	Description

BLAST of PI0028107 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZG8	4.0e-42	93.68	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G293290 PE=4 SV=1	[more]
A0A1S3BI57	5.0e-37	95.35	uncharacterized protein LOC103489916 OS=Cucumis melo OX=3656 GN=LOC103489916 PE=...	[more]
A0A6J1IHY2	2.1e-35	82.11	uncharacterized protein LOC111477620 OS=Cucurbita maxima OX=3661 GN=LOC111477620...	[more]
A0A6J1IAZ7	8.9e-34	79.17	uncharacterized protein LOC111471258 OS=Cucurbita maxima OX=3661 GN=LOC111471258...	[more]
A0A6J1HDK1	1.3e-32	77.89	uncharacterized protein LOC111463185 OS=Cucurbita moschata OX=3662 GN=LOC1114631...	[more]

BLAST of PI0028107 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142092.1	8.2e-42	93.68	uncharacterized protein LOC101219487 [Cucumis sativus] >KGN54219.1 hypothetical ...	[more]
XP_038883060.1	4.5e-40	90.72	uncharacterized protein LOC120074114 [Benincasa hispida]	[more]
XP_008447483.1	1.0e-36	95.35	PREDICTED: uncharacterized protein LOC103489916 [Cucumis melo]	[more]
XP_023544368.1	2.6e-35	83.16	uncharacterized protein LOC111803972 [Cucurbita pepo subsp. pepo] >KAG7033856.1 ...	[more]
XP_022977252.1	4.4e-35	82.11	uncharacterized protein LOC111477620 [Cucurbita maxima]	[more]

BLAST of PI0028107 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G02580.1	3.3e-25	65.79	Plant protein 1589 of unknown function	[more]
AT3G55240.1	1.7e-24	60.64	Plant protein 1589 of unknown function	[more]
AT3G28990.1	9.4e-20	59.21	Plant protein 1589 of unknown function	[more]
AT1G10657.2	5.4e-15	57.14	Plant protein 1589 of unknown function	[more]
AT1G10657.3	5.4e-15	57.14	Plant protein 1589 of unknown function	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006476	Conserved hypothetical protein CHP01589, plant	PFAM	PF09713	A_thal_3526	coord: 13..64 e-value: 1.1E-26 score: 92.9
IPR006476	Conserved hypothetical protein CHP01589, plant	TIGRFAM	TIGR01589	TIGR01589	coord: 10..65 e-value: 5.8E-25 score: 85.1
IPR006476	Conserved hypothetical protein CHP01589, plant	PANTHER	PTHR31871	OS02G0137100 PROTEIN	coord: 4..77
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 70..97
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 77..97
None	No IPR available	PANTHER	PTHR31871:SF18	ANGIOTENSIN-CONVERTING ENZYME 2-RELATED	coord: 4..77

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0028107.1	PI0028107.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity