PI0027685 (gene) Melon (PI 482460) v1

Overview


Name	PI0027685
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	O-methyltransferase family protein
Location	chr01: 4884625 .. 4885245 (+)
RNA-Seq Expression	PI0027685
Synteny	PI0027685

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACACTCTCCCAATTATCCACAGCTTTAAATTGTTCTTCCTCACCTCTATACCGCATCTTGAGATTCTTAGTCCGTCGTGGACTATTCAAACAAGAAATCACTGAAGAAAATGTCATAAGCTATGGCCAAACACCTTTGTCAAGGCTGCTTGCAAGTAACAACAATAACAGCATGGCTCCACTTCTTTTATTGGAGAGCAGCCCTGTGATGTTGGCACCATGGCATGGCCTTAGTGAACGAATTAAAGCCAATGAAGGAACCCCATTTGAGGCTGCTCATGGCAAAGATTTATGGAGCTTTACAGCAGCTGACACAACACATAACACAATATTTAATGACGGTATGGCATGTTCTGCCAGGGTTATTACTGTGCCAGCAATACTTGAAGATTGTCCACAGATTTTTGAAGGAATTGGAAGTTTAGTTTGATGTGGGAGGAGGAAATGGAACTTGCTTGAGCATGATTGTTAAGGCTTTCCTTGGATTAAAGGCATCAACTTTGACCTTCCACATGTCATTTCTTCTTCTCAACAATACATTGGTGTTCAATATATTGGTGGGAATATGTTTGATTCCATTCCTAAGGCTGATGCTGCTTTCTCATGGTTAGTATCGTAG

mRNA sequence

ATGACACTCTCCCAATTATCCACAGCTTTAAATTGTTCTTCCTCACCTCTATACCGCATCTTGAGATTCTTAGTCCGTCGTGGACTATTCAAACAAGAAATCACTGAAGAAAATGTCATAAGCTATGGCCAAACACCTTTGTCAAGGCTGCTTGCAAGTAACAACAATAACAGCATGGCTCCACTTCTTTTATTGGAGAGCAGCCCTGTGATGTTGGCACCATGGCATGGCCTTAGTGAACGAATTAAAGCCAATGAAGGAACCCCATTTGAGGCTGCTCATGGCAAAGATTTATGGAGCTTTACAGCAGCTGACACAACACATAACACAATATTTAATGACGGCATCAACTTTGACCTTCCACATGTCATTTCTTCTTCTCAACAATACATTGGTGTTCAATATATTGGTGGGAATATGTTTGATTCCATTCCTAAGGCTGATGCTGCTTTCTCATGGTTAGTATCGTAG

Coding sequence (CDS)

ATGACACTCTCCCAATTATCCACAGCTTTAAATTGTTCTTCCTCACCTCTATACCGCATCTTGAGATTCTTAGTCCGTCGTGGACTATTCAAACAAGAAATCACTGAAGAAAATGTCATAAGCTATGGCCAAACACCTTTGTCAAGGCTGCTTGCAAGTAACAACAATAACAGCATGGCTCCACTTCTTTTATTGGAGAGCAGCCCTGTGATGTTGGCACCATGGCATGGCCTTAGTGAACGAATTAAAGCCAATGAAGGAACCCCATTTGAGGCTGCTCATGGCAAAGATTTATGGAGCTTTACAGCAGCTGACACAACACATAACACAATATTTAATGACGGCATCAACTTTGACCTTCCACATGTCATTTCTTCTTCTCAACAATACATTGGTGTTCAATATATTGGTGGGAATATGTTTGATTCCATTCCTAAGGCTGATGCTGCTTTCTCATGGTTAGTATCGTAG

Protein sequence

MTLSQLSTALNCSSSPLYRILRFLVRRGLFKQEITEENVISYGQTPLSRLLASNNNNSMAPLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIFNDGINFDLPHVISSSQQYIGVQYIGGNMFDSIPKADAAFSWLVS

Homology

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BLAST of PI0027685 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.9e-22
Identity = 72/212 (33.96%), Postives = 108/212 (50.94%), Query Frame = 0


Query: 1   MTLSQLSTALNCSSSPLYRILRFLVRRGLFKQEITEENVIS-YGQTPLSR--LLASNNNN 60
           +TL++LS+A++ S S L RI+RFLV +G+FK+  T++ + + Y  TPLSR  ++   +  
Sbjct: 67  VTLAELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGK 126

Query: 61  SMAPLLLLESSPVMLAPWHGLSERIKA--NEGT--PFEAAHGKDLWSFTAADTTHNTIFN 120
           S+AP +L E++P MLAPW  LS  + +  N  T  PF+A HGKD+WSF   +   + + N
Sbjct: 127 SLAPFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMIN 186

Query: 121 D-------------------------------------------------GINFDLPHVI 156
           +                                                 G NFDLPHVI
Sbjct: 187 EAMACDARRVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVI 246

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BLAST of PI0027685 vs. ExPASy Swiss-Prot
Match: B0ZB55 (Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 PE=1 SV=2)

HSP 1 Score: 100.9 bits (250), Expect = 1.3e-20
Identity = 68/209 (32.54%), Postives = 105/209 (50.24%), Query Frame = 0


Query: 1   MTLSQLSTAL----NCSSSP----LYRILRFLVRRGLF--KQEITEENVISYGQTPLSRL 60
           +TLSQ+++ +    N S SP    L RI+RFLVR+ +F   QE  +E +  YG +  SR 
Sbjct: 42  ITLSQIASGIRSNSNSSISPNIPYLSRIMRFLVRKNIFTEHQEDNDEVISLYGLSDSSRW 101

Query: 61  LASNNNNSMAPLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNT 120
           L  +  +S+AP++L+++ P+ +A WH L + ++ N    FE AHG ++W F++A+   N 
Sbjct: 102 LLRDFKSSLAPMVLMQTHPLSMAVWHFLEDYVR-NSSNTFEKAHGCNIWEFSSANPDFNK 161

Query: 121 IFND--------------------------------------------------GINFDL 150
           IFN+                                                  GINFDL
Sbjct: 162 IFNNAMASIVPIYMGAVLSSYKDGLGCIKGTVVDVGGGTGGSISELMKYYPNIKGINFDL 221

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BLAST of PI0027685 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.1e-18
Identity = 63/214 (29.44%), Postives = 102/214 (47.66%), Query Frame = 0


Query: 1   MTLSQLSTALNC---SSSP----LYRILRFLVRRGLFKQEITEE-NVISYGQTPLSRLLA 60
           +T+S++  +L     SSSP    L RI+R LV + LF  E+ +E N + Y  T  S+ L 
Sbjct: 46  VTISEIVDSLKTNTPSSSPNIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLL 105

Query: 61  SNNNNSMAPLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIF 120
            ++  +++PL+L E++P++L PW  L  +    + +PFE AHG ++W    AD   N   
Sbjct: 106 KDSKFNLSPLVLWETNPILLKPWQYLG-KCAQEKSSPFERAHGCEIWDLALADPKFNNFL 165

Query: 121 N--------------------------------------------------DGINFDLPH 156
           N                                                   GINFDLPH
Sbjct: 166 NGAMQCSTTTIINEMLLEYKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPH 225

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BLAST of PI0027685 vs. ExPASy Swiss-Prot
Match: P45986 (Inositol 4-methyltransferase OS=Mesembryanthemum crystallinum OX=3544 GN=IMT1 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.3e-15
Identity = 54/164 (32.93%), Postives = 74/164 (45.12%), Query Frame = 0


Query: 42  YGQTPLSRLLASNN-NNSMAPLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWS 101
           YG  PL   LASN+   S+ PLL+L    VM+  W  L++ I    G PF+ AHG   + 
Sbjct: 107 YGPAPLCNYLASNDGQGSLGPLLVLHHDKVMMESWFHLNDYI-LEGGVPFKRAHGMIQFD 166

Query: 102 FTAADTTHNTIFND---------------------------------------------- 156
           +T  D   N +FN                                               
Sbjct: 167 YTGTDERFNHVFNQGMAHHTILVMKKLLDNYNGFNDVKVLVDVGGNIGVNVSMIVAKHTH 226

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BLAST of PI0027685 vs. ExPASy Swiss-Prot
Match: Q9LEL5 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 4.9e-15
Identity = 56/209 (26.79%), Postives = 90/209 (43.06%), Query Frame = 0


Query: 1   MTLSQLSTAL-----NCSSSPLYRILRFLVRRGLFKQEITEENVISYGQTPLSRLLASNN 60
           M L+ L++ L     NC +  LYRILR+LV+  + + E +++    Y   P++ LL+ N 
Sbjct: 50  MALADLASKLPVSDVNCDN--LYRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNA 109

Query: 61  NNSMAPLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIFNDG 120
             SM P++L  +    + PWH + + +  N GT FE A G  +W +       + +FN+G
Sbjct: 110 KRSMVPMILGMTQKDFMTPWHSMKDGLSDN-GTAFEKAMGMTIWEYLEGHPDQSQLFNEG 169

Query: 121 IN-------------------------------------------------FDLPHVISS 156
           +                                                  FDLPHVI++
Sbjct: 170 MAGETRLLTSSLISGSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIAN 229

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BLAST of PI0027685 vs. ExPASy TrEMBL
Match: A0A5D3DJ08 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004240 PE=3 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.6e-59
Identity = 130/201 (64.68%), Postives = 137/201 (68.16%), Query Frame = 0


Query: 1   MTLSQLSTALNCSSSPLYRILRFLVRRGLFKQEITEENVISYGQTPLSRLLASNNNNSMA 60
           MTLSQLSTALNCSSS LYRILRFLVRRG+FKQEITEENVISY  TPLSRLLAS+NNNSMA
Sbjct: 53  MTLSQLSTALNCSSSLLYRILRFLVRRGIFKQEITEENVISYDHTPLSRLLASSNNNSMA 112

Query: 61  PLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIFND------ 120
           PLLLLESSPVMLAPWH LS RIK N  TPFEAAHGKD+WSF AAD  HN + ND      
Sbjct: 113 PLLLLESSPVMLAPWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTA 172

Query: 121 --------------------------------------------GINFDLPHVISSSQQY 152
                                                       GINFDLPHVISSSQQY
Sbjct: 173 RVHTVPAILEDCPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQY 232

[Close]

BLAST of PI0027685 vs. ExPASy TrEMBL
Match: A0A1S3BII4 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490252 PE=3 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.6e-59
Identity = 130/201 (64.68%), Postives = 137/201 (68.16%), Query Frame = 0


Query: 1   MTLSQLSTALNCSSSPLYRILRFLVRRGLFKQEITEENVISYGQTPLSRLLASNNNNSMA 60
           MTLSQLSTALNCSSS LYRILRFLVRRG+FKQEITEENVISY  TPLSRLLAS+NNNSMA
Sbjct: 53  MTLSQLSTALNCSSSLLYRILRFLVRRGIFKQEITEENVISYDHTPLSRLLASSNNNSMA 112

Query: 61  PLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIFND------ 120
           PLLLLESSPVMLAPWH LS RIK N  TPFEAAHGKD+WSF AAD  HN + ND      
Sbjct: 113 PLLLLESSPVMLAPWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTA 172

Query: 121 --------------------------------------------GINFDLPHVISSSQQY 152
                                                       GINFDLPHVISSSQQY
Sbjct: 173 RVHTVPAILEDCPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQY 232

[Close]

BLAST of PI0027685 vs. ExPASy TrEMBL
Match: A0A0A0K2N4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G017720 PE=3 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 8.3e-58
Identity = 127/201 (63.18%), Postives = 135/201 (67.16%), Query Frame = 0


Query: 1   MTLSQLSTALNCSSSPLYRILRFLVRRGLFKQEITEENVISYGQTPLSRLLASNNNNSMA 60
           MTLSQLSTALNCS+S LYRILRFLVRRG+FKQEI E NVISY QTPLSRLLAS+NNNSMA
Sbjct: 55  MTLSQLSTALNCSASLLYRILRFLVRRGIFKQEINEANVISYDQTPLSRLLASSNNNSMA 114

Query: 61  PLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIFND------ 120
           P LLLESSPVMLAPWH LS RIK N  TPFEAAHGKD+WSF AAD  HN + ND      
Sbjct: 115 PFLLLESSPVMLAPWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTA 174

Query: 121 --------------------------------------------GINFDLPHVISSSQQY 152
                                                       GINFDLPHVISSSQQY
Sbjct: 175 RVLTVPAILEECPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQY 234

[Close]

BLAST of PI0027685 vs. ExPASy TrEMBL
Match: A0A6J1GLX2 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455163 PE=3 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 6.2e-53
Identity = 113/201 (56.22%), Postives = 134/201 (66.67%), Query Frame = 0


Query: 1   MTLSQLSTALNCSSSPLYRILRFLVRRGLFKQEITEENVISYGQTPLSRLLASNNNNSMA 60
           MTL +LS++LNCSSS LYRI+RFLV RG+FK+EIT+EN+  Y  TPLSRLLAS++++SMA
Sbjct: 54  MTLPELSSSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMA 113

Query: 61  PLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIFND------ 120
           PLLLLES+PVMLAPWHGLS RIK NE  PFEAAHGKD+WS+ AA+ THN +FND      
Sbjct: 114 PLLLLESNPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSA 173

Query: 121 --------------------------------------------GINFDLPHVISSSQQY 152
                                                       GINFDLPHV++SS  Y
Sbjct: 174 RVMTVPAILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVVASSPPY 233

[Close]

BLAST of PI0027685 vs. ExPASy TrEMBL
Match: A0A6J1C2I8 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica charantia OX=3673 GN=LOC111007866 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.4e-52
Identity = 115/201 (57.21%), Postives = 130/201 (64.68%), Query Frame = 0


Query: 1   MTLSQLSTALNCSSSPLYRILRFLVRRGLFKQEITEENVISYGQTPLSRLLASNNNNSMA 60
           MTLSQLS+ALNCS   LYRILRFL  RG+FK+E T +N+  Y QTPLSRLLASN NNSMA
Sbjct: 55  MTLSQLSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMA 114

Query: 61  PLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIFND------ 120
           PLLLLESSP+MLA WH LS RIKA++GTPFEA HGKDLWS+TAAD THN IFND      
Sbjct: 115 PLLLLESSPIMLASWHNLSARIKASDGTPFEATHGKDLWSYTAADPTHNRIFNDAMACAA 174

Query: 121 --------------------------------------------GINFDLPHVISSSQQY 152
                                                       GINFDLPHV+S +Q+ 
Sbjct: 175 RVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQEC 234

[Close]

BLAST of PI0027685 vs. NCBI nr
Match: KAA0040344.1 ((R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK23360.1 (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 238.0 bits (606), Expect = 5.3e-59
Identity = 130/201 (64.68%), Postives = 137/201 (68.16%), Query Frame = 0


Query: 1   MTLSQLSTALNCSSSPLYRILRFLVRRGLFKQEITEENVISYGQTPLSRLLASNNNNSMA 60
           MTLSQLSTALNCSSS LYRILRFLVRRG+FKQEITEENVISY  TPLSRLLAS+NNNSMA
Sbjct: 53  MTLSQLSTALNCSSSLLYRILRFLVRRGIFKQEITEENVISYDHTPLSRLLASSNNNSMA 112

Query: 61  PLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIFND------ 120
           PLLLLESSPVMLAPWH LS RIK N  TPFEAAHGKD+WSF AAD  HN + ND      
Sbjct: 113 PLLLLESSPVMLAPWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTA 172

Query: 121 --------------------------------------------GINFDLPHVISSSQQY 152
                                                       GINFDLPHVISSSQQY
Sbjct: 173 RVHTVPAILEDCPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQY 232

[Close]

BLAST of PI0027685 vs. NCBI nr
Match: XP_008447906.1 (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 238.0 bits (606), Expect = 5.3e-59
Identity = 130/201 (64.68%), Postives = 137/201 (68.16%), Query Frame = 0


Query: 1   MTLSQLSTALNCSSSPLYRILRFLVRRGLFKQEITEENVISYGQTPLSRLLASNNNNSMA 60
           MTLSQLSTALNCSSS LYRILRFLVRRG+FKQEITEENVISY  TPLSRLLAS+NNNSMA
Sbjct: 53  MTLSQLSTALNCSSSLLYRILRFLVRRGIFKQEITEENVISYDHTPLSRLLASSNNNSMA 112

Query: 61  PLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIFND------ 120
           PLLLLESSPVMLAPWH LS RIK N  TPFEAAHGKD+WSF AAD  HN + ND      
Sbjct: 113 PLLLLESSPVMLAPWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTA 172

Query: 121 --------------------------------------------GINFDLPHVISSSQQY 152
                                                       GINFDLPHVISSSQQY
Sbjct: 173 RVHTVPAILEDCPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQY 232

[Close]

BLAST of PI0027685 vs. NCBI nr
Match: XP_004144927.1 (acetylserotonin O-methyltransferase [Cucumis sativus] >KGN43289.1 hypothetical protein Csa_020398 [Cucumis sativus])

HSP 1 Score: 233.0 bits (593), Expect = 1.7e-57
Identity = 127/201 (63.18%), Postives = 135/201 (67.16%), Query Frame = 0


Query: 1   MTLSQLSTALNCSSSPLYRILRFLVRRGLFKQEITEENVISYGQTPLSRLLASNNNNSMA 60
           MTLSQLSTALNCS+S LYRILRFLVRRG+FKQEI E NVISY QTPLSRLLAS+NNNSMA
Sbjct: 55  MTLSQLSTALNCSASLLYRILRFLVRRGIFKQEINEANVISYDQTPLSRLLASSNNNSMA 114

Query: 61  PLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIFND------ 120
           P LLLESSPVMLAPWH LS RIK N  TPFEAAHGKD+WSF AAD  HN + ND      
Sbjct: 115 PFLLLESSPVMLAPWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTA 174

Query: 121 --------------------------------------------GINFDLPHVISSSQQY 152
                                                       GINFDLPHVISSSQQY
Sbjct: 175 RVLTVPAILEECPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQY 234

[Close]

BLAST of PI0027685 vs. NCBI nr
Match: XP_038888665.1 (acetylserotonin O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 232.6 bits (592), Expect = 2.2e-57
Identity = 126/201 (62.69%), Postives = 136/201 (67.66%), Query Frame = 0


Query: 1   MTLSQLSTALNCSSSPLYRILRFLVRRGLFKQEITEENVISYGQTPLSRLLASNNNNSMA 60
           MTLSQLS ALNCSSS LYRILRFLV RG+FKQEITEEN+ SY QTPLSRLLAS+NNNSM 
Sbjct: 55  MTLSQLSLALNCSSSLLYRILRFLVNRGIFKQEITEENLTSYSQTPLSRLLASSNNNSMV 114

Query: 61  PLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIFND------ 120
           PL+LLESSPVMLAPWH LS+RIKANEGTPFEAAHGKDLWSF AAD  HNTI N+      
Sbjct: 115 PLVLLESSPVMLAPWHRLSDRIKANEGTPFEAAHGKDLWSFAAADPIHNTIINEAMSCTA 174

Query: 121 --------------------------------------------GINFDLPHVISSSQQY 152
                                                       GIN DLPHVISSSQ+Y
Sbjct: 175 RVLTVPAILEDCPEIFEGIGSLVDVGGGNGTCLSMIVKACPWIKGINLDLPHVISSSQEY 234

[Close]

BLAST of PI0027685 vs. NCBI nr
Match: XP_038888695.1 (acetylserotonin O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 226.9 bits (577), Expect = 1.2e-55
Identity = 121/201 (60.20%), Postives = 135/201 (67.16%), Query Frame = 0


Query: 1   MTLSQLSTALNCSSSPLYRILRFLVRRGLFKQEITEENVISYGQTPLSRLLASNNNNSMA 60
           MTLSQLS+ALNCSSS LYRI+RFLV RG+FKQEIT+EN+ SY QTPLSRLLAS++ NSMA
Sbjct: 54  MTLSQLSSALNCSSSLLYRIMRFLVPRGIFKQEITQENLTSYAQTPLSRLLASSSKNSMA 113

Query: 61  PLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIFND------ 120
           P LLLES+PVMLAPWHGLS RIK    TPFEAAHGKD+WSF AAD  HN + ND      
Sbjct: 114 PFLLLESNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTA 173

Query: 121 --------------------------------------------GINFDLPHVISSSQQY 152
                                                       GINFDLPHVISSSQ+Y
Sbjct: 174 RVLTVPAILEDCPKIFEGIGSLVDVGGGNGTCLSMIVKTCPWIKGINFDLPHVISSSQEY 233

[Close]

BLAST of PI0027685 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 107.1 bits (266), Expect = 1.3e-23
Identity = 72/212 (33.96%), Postives = 108/212 (50.94%), Query Frame = 0


Query: 1   MTLSQLSTALNCSSSPLYRILRFLVRRGLFKQEITEENVIS-YGQTPLSR--LLASNNNN 60
           +TL++LS+A++ S S L RI+RFLV +G+FK+  T++ + + Y  TPLSR  ++   +  
Sbjct: 67  VTLAELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGK 126

Query: 61  SMAPLLLLESSPVMLAPWHGLSERIKA--NEGT--PFEAAHGKDLWSFTAADTTHNTIFN 120
           S+AP +L E++P MLAPW  LS  + +  N  T  PF+A HGKD+WSF   +   + + N
Sbjct: 127 SLAPFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMIN 186

Query: 121 D-------------------------------------------------GINFDLPHVI 156
           +                                                 G NFDLPHVI
Sbjct: 187 EAMACDARRVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVI 246

[Close]

BLAST of PI0027685 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 71.2 bits (173), Expect = 8.1e-13
Identity = 57/206 (27.67%), Postives = 82/206 (39.81%), Query Frame = 0


Query: 1   MTLSQLSTALNCSSSPLYRILRFLVRRGLFKQEITEENVIS-YGQTPLSRLLASNNNNSM 60
           +TLS+LS+A++ S S L RI+RFLV +GLFK+  T++ + + Y  TPLSR +        
Sbjct: 53  VTLSELSSAVSASPSHLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMM------- 112

Query: 61  APLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIFND----- 120
                                             HGKDLW+F   +  H+ + N+     
Sbjct: 113 ------------------------------ITKLHGKDLWAFAQDNLCHSQLINEAMACD 172

Query: 121 --------------------------------------------GINFDLPHVISSSQQY 156
                                                       G NFDLPHVI  +Q  
Sbjct: 173 ARRVVPRVAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVL 221

[Close]

BLAST of PI0027685 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 67.8 bits (164), Expect = 8.9e-12
Identity = 44/163 (26.99%), Postives = 68/163 (41.72%), Query Frame = 0


Query: 42  YGQTPLSRLLASNNNN-SMAPLLLLESSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWS 101
           YG  P+ + L  N +  S+A L L+    V++  W+ L + I  + G PF  A+G   + 
Sbjct: 104 YGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAI-LDGGIPFNKAYGMSAFE 163

Query: 102 FTAADTTHNTIFND---------------------------------------------- 155
           +   D   N +FN+                                              
Sbjct: 164 YHGTDPRFNKVFNNGMSNHSTITMKKILETYKGFEGLTSLVDVGGGIGATLKMIVSKYPN 223

[Close]

BLAST of PI0027685 vs. TAIR 10
Match: AT5G53810.1 (O-methyltransferase family protein )

HSP 1 Score: 58.9 bits (141), Expect = 4.1e-09
Identity = 52/198 (26.26%), Postives = 80/198 (40.40%), Query Frame = 0


Query: 17  LYRILRFLVRRGLFK-QEITEENVIS------YGQTPLSRLLASNNNN---SMAPLLLLE 76
           L R+LRFLV   + K + + EEN  +      Y   P+ + L + +++   S A L +L+
Sbjct: 86  LDRMLRFLVSHSVLKCRTVIEENGQTGKVERVYAAEPVCKYLLNKSDDVSGSFASLFMLD 145

Query: 77  SSPVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIFND------------ 136
            S V +  W  L + I       F +AHG  L+ +  AD     +FN             
Sbjct: 146 LSDVFIKTWTHLEDVILEGRDA-FSSAHGMKLFEYIQADERFGKVFNRAMLESSTMVTEK 205

Query: 137 ------------------------------------GINFDLPHVISSSQQYIGVQYIGG 156
                                               GINFDL  V++++  Y GV ++ G
Sbjct: 206 VLKFYEGFKDVKTLVDVGGGLGNTLGLITSKYPHLIGINFDLAPVLANAHSYPGVNHVAG 265

[Close]

BLAST of PI0027685 vs. TAIR 10
Match: AT1G33030.1 (O-methyltransferase family protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.2e-08
Identity = 48/197 (24.37%), Postives = 77/197 (39.09%), Query Frame = 0


Query: 14  SSPLYRILRFLVRRGLFKQEITEEN---VISYGQTPLSRLLASNNN--NSMAPLLLLESS 73
           SS + RILRFL    +    ++ E+      YG  P+++    N N   S+AP++ L   
Sbjct: 60  SSLVNRILRFLASYSILTCSVSTEHGEPFAIYGLAPVAKYFTKNQNGGGSLAPMVNLFQD 119

Query: 74  PVMLAPWHGLSERIKANEGTPFEAAHGKDLWSFTAADTTHNTIF---------------- 133
            V+   W+ L + +    G PF   HG        +D+    +F                
Sbjct: 120 KVVTDMWYNLKDSV-LEGGLPFNNTHGSSAVELVGSDSRFREVFQSSMKGFNEVFIEEFL 179

Query: 134 -----------------NDG----------------INFDLPHVISSSQQYIGVQYIGGN 156
                             DG                INFDLP VI++S    G++++ G+
Sbjct: 180 KNYNGFDGVKSLVDVGGGDGSLLSRIISKHTHIIKAINFDLPTVINTSLPSPGIEHVAGD 239

The following BLAST results are available for this feature:

BLAST of PI0027685 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9T003	1.9e-22	33.96	Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1...	[more]
B0ZB55	1.3e-20	32.54	Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 P...	[more]
Q6WUC2	2.1e-18	29.44	(R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=...	[more]
P45986	1.3e-15	32.93	Inositol 4-methyltransferase OS=Mesembryanthemum crystallinum OX=3544 GN=IMT1 PE...	[more]
Q9LEL5	4.9e-15	26.79	3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica OX=...	[more]

BLAST of PI0027685 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DJ08	2.6e-59	64.68	(R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3BII4	2.6e-59	64.68	(R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490...	[more]
A0A0A0K2N4	8.3e-58	63.18	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G017720 PE=3 SV=1	[more]
A0A6J1GLX2	6.2e-53	56.22	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1C2I8	1.4e-52	57.21	3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica ch...	[more]

BLAST of PI0027685 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0040344.1	5.3e-59	64.68	(R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK23360...	[more]
XP_008447906.1	5.3e-59	64.68	PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo]	[more]
XP_004144927.1	1.7e-57	63.18	acetylserotonin O-methyltransferase [Cucumis sativus] >KGN43289.1 hypothetical p...	[more]
XP_038888665.1	2.2e-57	62.69	acetylserotonin O-methyltransferase-like [Benincasa hispida]	[more]
XP_038888695.1	1.2e-55	60.20	acetylserotonin O-methyltransferase-like [Benincasa hispida]	[more]

BLAST of PI0027685 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	1.3e-23	33.96	O-methyltransferase family protein	[more]
AT4G35150.1	8.1e-13	27.67	O-methyltransferase family protein	[more]
AT5G54160.1	8.9e-12	26.99	O-methyltransferase 1	[more]
AT5G53810.1	4.1e-09	26.26	O-methyltransferase family protein	[more]
AT1G33030.1	1.2e-08	24.37	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 116..155 e-value: 8.2E-7 score: 30.2
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 52..115 e-value: 9.0E-10 score: 39.9
None	No IPR available	PANTHER	PTHR11746:SF191	3'-HYDROXY-N-METHYL-(S)-COCLAURINE 4'-O-METHYLTRANSFERASE 2-LIKE	coord: 2..115
None	No IPR available	PANTHER	PTHR11746:SF191	3'-HYDROXY-N-METHYL-(S)-COCLAURINE 4'-O-METHYLTRANSFERASE 2-LIKE	coord: 114..152
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 1..51 e-value: 5.5E-9 score: 37.7
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 115..151 e-value: 1.5E-6 score: 27.7
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 114..152
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 2..115
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 1..156 score: 17.317019
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 1..55
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 56..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0027685.1	PI0027685.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity