PI0027509 (gene) Melon (PI 482460) v1

Overview


Name	PI0027509
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chr04: 7581141 .. 7582375 (-)
RNA-Seq Expression	PI0027509
Synteny	PI0027509

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCAATGTGGGATTCCAATTCCAATTTCAAAGCAGTAAATGAAGAAGAAGATCATCCATGGAAGGAAGCCATAAACAAAGGCCATGGAGCGACAGGGCTTTCCGAAATGCGTCTTAGAGCACTCCCTAAAGCATTCATTCAGCCTCCAGAAAAGAGATTGAGCTCGATGATCAGAGCGTCACTGGCATCAGGAGACACAATACCGGTAATCGACATGTCGAAAGCAGAAGCAGCAGAATTAATCTGTGAGGCGGCGGAGAAATGGGGGTTTTTCCAGGTGGTAAACCATGGAGTTCCGGCGGCATTGATGAGCAATGTAATGCAGGCAGCTCGGAGATTCTTCGGTCAGGCGGCCGAAGAGAAGAGAAGGTTTTTGAAGGAGAATTCGGGTTGTTCAGATCATGTGATTTATGGGACAAGCTTTTTCATTGAGGCAGAGAAAGCTTTAGAGTGGAGCGATCACTTGACTATGCCTTTTGTTTCTGAGGATGAAGCTTCTGCATTCTGGCCTGCTGTTTCCAAGTAATCAATCAAACATTACAATTCACTTTTGATTCTCTTCGCATATTTCCTATTTGGTTTTTCGTTTTTAAATATTAAGTGTTGTAAATTAGTCTTAAATTAAGTCCATAATTGATAAGTATGGTTTGACATTGCAGAGATGCAACAATTGAATATCTAAAGAGCTCAGACACATTGATCAAACGAGTATTAAAGATTCTAATGAACAAACTAAAGGCCAATATTGAACTCGAGGAAACAAATCAATGTCCCACAATCCAATTCAACTACTATCCCATCTGTCCAAACCCCGATCTTAGCATCGGAGTCCGTAGCCACTCCGACATTTCACTGGTGACGGTTCTGCTGCAAGACGATGTCGGCGGACTCCACGTGCGACGAGAAATTGATAGCGGAGGAGAGGAATGGGTTCATGTGAAGCCAGTAGCAGAGTCTTTAGTAATCAACATTGGGGATTCTTTAGAGATAATGAGCAATGGAATTTACAAGAGCGCGGAGCATTGTGTGGCGTCCAATGGAAGTAGCGATAGAATATCAATTCCCATATTTGTCTTTCCGGAAAAATCTTCCGTTGTTGGGCCGCTGCCGGAGGTGCTTGCGACGGGGAAGAAAGCAGAGTACAAGGAAGTTGTGTTTTCAGATTATTTTAAGCATTTCTTGAGCAAGCCACTTGATGGGAAGAACAACCTAGAGTTAGCCAAAATATAA

mRNA sequence

ATGGCTTCAATGTGGGATTCCAATTCCAATTTCAAAGCAGTAAATGAAGAAGAAGATCATCCATGGAAGGAAGCCATAAACAAAGGCCATGGAGCGACAGGGCTTTCCGAAATGCGTCTTAGAGCACTCCCTAAAGCATTCATTCAGCCTCCAGAAAAGAGATTGAGCTCGATGATCAGAGCGTCACTGGCATCAGGAGACACAATACCGGTAATCGACATGTCGAAAGCAGAAGCAGCAGAATTAATCTGTGAGGCGGCGGAGAAATGGGGGTTTTTCCAGGTGGTAAACCATGGAGTTCCGGCGGCATTGATGAGCAATGTAATGCAGGCAGCTCGGAGATTCTTCGGTCAGGCGGCCGAAGAGAAGAGAAGGTTTTTGAAGGAGAATTCGGGTTGTTCAGATCATGTGATTTATGGGACAAGCTTTTTCATTGAGGCAGAGAAAGCTTTAGAGTGGAGCGATCACTTGACTATGCCTTTTGTTTCTGAGGATGAAGCTTCTGCATTCTGGCCTGCTGTTTCCAAAGATGCAACAATTGAATATCTAAAGAGCTCAGACACATTGATCAAACGAGTATTAAAGATTCTAATGAACAAACTAAAGGCCAATATTGAACTCGAGGAAACAAATCAATGTCCCACAATCCAATTCAACTACTATCCCATCTGTCCAAACCCCGATCTTAGCATCGGAGTCCGTAGCCACTCCGACATTTCACTGGTGACGGTTCTGCTGCAAGACGATGTCGGCGGACTCCACGTGCGACGAGAAATTGATAGCGGAGGAGAGGAATGGGTTCATGTGAAGCCAGTAGCAGAGTCTTTAGTAATCAACATTGGGGATTCTTTAGAGATAATGAGCAATGGAATTTACAAGAGCGCGGAGCATTGTGTGGCGTCCAATGGAAGTAGCGATAGAATATCAATTCCCATATTTGTCTTTCCGGAAAAATCTTCCGTTGTTGGGCCGCTGCCGGAGGTGCTTGCGACGGGGAAGAAAGCAGAGTACAAGGAAGTTGTGTTTTCAGATTATTTTAAGCATTTCTTGAGCAAGCCACTTGATGGGAAGAACAACCTAGAGTTAGCCAAAATATAA

Coding sequence (CDS)

ATGGCTTCAATGTGGGATTCCAATTCCAATTTCAAAGCAGTAAATGAAGAAGAAGATCATCCATGGAAGGAAGCCATAAACAAAGGCCATGGAGCGACAGGGCTTTCCGAAATGCGTCTTAGAGCACTCCCTAAAGCATTCATTCAGCCTCCAGAAAAGAGATTGAGCTCGATGATCAGAGCGTCACTGGCATCAGGAGACACAATACCGGTAATCGACATGTCGAAAGCAGAAGCAGCAGAATTAATCTGTGAGGCGGCGGAGAAATGGGGGTTTTTCCAGGTGGTAAACCATGGAGTTCCGGCGGCATTGATGAGCAATGTAATGCAGGCAGCTCGGAGATTCTTCGGTCAGGCGGCCGAAGAGAAGAGAAGGTTTTTGAAGGAGAATTCGGGTTGTTCAGATCATGTGATTTATGGGACAAGCTTTTTCATTGAGGCAGAGAAAGCTTTAGAGTGGAGCGATCACTTGACTATGCCTTTTGTTTCTGAGGATGAAGCTTCTGCATTCTGGCCTGCTGTTTCCAAAGATGCAACAATTGAATATCTAAAGAGCTCAGACACATTGATCAAACGAGTATTAAAGATTCTAATGAACAAACTAAAGGCCAATATTGAACTCGAGGAAACAAATCAATGTCCCACAATCCAATTCAACTACTATCCCATCTGTCCAAACCCCGATCTTAGCATCGGAGTCCGTAGCCACTCCGACATTTCACTGGTGACGGTTCTGCTGCAAGACGATGTCGGCGGACTCCACGTGCGACGAGAAATTGATAGCGGAGGAGAGGAATGGGTTCATGTGAAGCCAGTAGCAGAGTCTTTAGTAATCAACATTGGGGATTCTTTAGAGATAATGAGCAATGGAATTTACAAGAGCGCGGAGCATTGTGTGGCGTCCAATGGAAGTAGCGATAGAATATCAATTCCCATATTTGTCTTTCCGGAAAAATCTTCCGTTGTTGGGCCGCTGCCGGAGGTGCTTGCGACGGGGAAGAAAGCAGAGTACAAGGAAGTTGTGTTTTCAGATTATTTTAAGCATTTCTTGAGCAAGCCACTTGATGGGAAGAACAACCTAGAGTTAGCCAAAATATAA

Protein sequence

MASMWDSNSNFKAVNEEEDHPWKEAINKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSKAEAAELICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGTSFFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKLKANIELEETNQCPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVRREIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFPEKSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI

Homology

[Close]

BLAST of PI0027509 vs. ExPASy Swiss-Prot
Match: G9M9M3 (Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-2-1 OS=Ipomoea batatas OX=4120 GN=F6H2-2-1 PE=1 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 3.9e-95
Identity = 178/348 (51.15%), Postives = 243/348 (69.83%), Query Frame = 0


Query: 26  INKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSKAE---AAEL 85
           + +GHG  GLSE+ L+ LP  ++ PPE+RLSSM    + S D+IPVID+S  E    A+L
Sbjct: 18  VKQGHGVKGLSELGLQTLPNQYVHPPEERLSSM---DVVSDDSIPVIDVSNWEDPKVAKL 77

Query: 86  ICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGTS 145
           IC+AAEK GFFQ+VNHG+P  ++     A  RFF + AEEK+++ KEN   S HV Y TS
Sbjct: 78  ICDAAEKRGFFQIVNHGIPIEMLEKAKAATYRFFREPAEEKKKYSKENCPTS-HVRYSTS 137

Query: 146 FFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKLK 205
           F  + EKALEW DHL+M +VS++EA+ +WP   +D  +EYLKS + + +++L+ LM  L 
Sbjct: 138 FLPQIEKALEWKDHLSMFYVSDEEAAQYWPPSCRDDAVEYLKSCEMVSRKLLEALMQGLN 197

Query: 206 ANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVRR 265
            N +++++ +        I  NYYP CPNPDL++GV  HSDIS +T+LLQDD+GGL+VR+
Sbjct: 198 VN-QIDDSKESLLMGSRRININYYPKCPNPDLTVGVGRHSDISTLTLLLQDDIGGLYVRK 257

Query: 266 EIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFPE 325
                 E W HV PV  +LVINIGD+L+IMSNG YKS EH V +N S+DRIS+P+FV P 
Sbjct: 258 ---LEHEAWSHVPPVKGALVINIGDALQIMSNGRYKSIEHRVMANESNDRISVPVFVNPR 317

Query: 326 KSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
            + +VGPLPEVLA+G+K  YK V++SDY KHF  K  +GK+ +  A+I
Sbjct: 318 PNDIVGPLPEVLASGEKPVYKPVLYSDYAKHFYRKAHNGKDTIAFARI 357

[Close]

BLAST of PI0027509 vs. ExPASy Swiss-Prot
Match: G9M9M4 (Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-1-1 OS=Ipomoea batatas OX=4120 GN=F6H2-1-1 PE=1 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 5.0e-95
Identity = 178/348 (51.15%), Postives = 243/348 (69.83%), Query Frame = 0


Query: 26  INKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSKAE---AAEL 85
           + +GHG  GLSE+ L+ LP  ++ PPE+RLSSM    + S D+IPVID+S  E    A+L
Sbjct: 18  VKQGHGVKGLSELGLQTLPNQYVHPPEERLSSM---DVVSDDSIPVIDVSNWEDPKVAKL 77

Query: 86  ICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGTS 145
           IC+AAEK GFFQ+VNHG+P  ++     A  RFF + AEEK+++ KEN   S HV Y TS
Sbjct: 78  ICDAAEKRGFFQIVNHGIPLEMLEKAKAATYRFFREPAEEKKKYSKENCPTS-HVRYSTS 137

Query: 146 FFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKLK 205
           F  + EKALEW DHL+M +VS++EA+ +WP   +D  +EYLKS + + +++L+ LM  L 
Sbjct: 138 FLPQIEKALEWKDHLSMFYVSDEEAAQYWPPSCRDDALEYLKSCEMVSRKLLEALMQGLN 197

Query: 206 ANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVRR 265
            N E+++  +        I  NYYP CPNPDL++GV  HSDIS +T+LLQDD+GGL+VR+
Sbjct: 198 VN-EIDDAKESLLMGSRRININYYPKCPNPDLTVGVGRHSDISTLTLLLQDDIGGLYVRK 257

Query: 266 EIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFPE 325
                 E W HV PV  +LVINIGD+L+IMSNG YKS EH V +N ++DRIS+P+FV P+
Sbjct: 258 ---LEHEAWSHVPPVKGALVINIGDALQIMSNGRYKSIEHRVLANETNDRISVPVFVNPK 317

Query: 326 KSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
            + +VGPLPEVLA+G+K  YK V++SDY KHF  K  +GK+ +  A+I
Sbjct: 318 PNDIVGPLPEVLASGEKPVYKPVLYSDYAKHFYRKAHNGKDTIAFARI 357

[Close]

BLAST of PI0027509 vs. ExPASy Swiss-Prot
Match: G9M9M5 (Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-1-8 OS=Ipomoea batatas OX=4120 GN=F6H2-1-8 PE=1 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 2.5e-94
Identity = 177/348 (50.86%), Postives = 242/348 (69.54%), Query Frame = 0


Query: 26  INKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSKAE---AAEL 85
           + +GHG  GLSE+ L+ LP  ++ PPE+RLSSM    + S D+IPVID+S  E    A+L
Sbjct: 19  VKQGHGVKGLSELGLQTLPNQYVHPPEERLSSM---DVVSDDSIPVIDVSNWEDPKVAKL 78

Query: 86  ICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGTS 145
           IC AAEK GFFQ+VNHG+P  ++     A  RFF + A+EK+++ KEN   S HV Y TS
Sbjct: 79  ICNAAEKRGFFQIVNHGIPLEMLEKAKAATYRFFREPAQEKKKYSKENCPTS-HVRYSTS 138

Query: 146 FFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKLK 205
           F  + EKALEW DHL+M +VS+ EA+ +WP   +D  +EYLKS + + +++L+ LM  L 
Sbjct: 139 FLPQIEKALEWKDHLSMFYVSDQEAAQYWPPSCRDDALEYLKSCELVSRKLLEALMQGLN 198

Query: 206 ANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVRR 265
            N +++++ +        I  NYYP CPNPDL++GV  HSDIS +T+LLQDD+GGL+VR+
Sbjct: 199 VN-QIDDSKESLLMGSRRININYYPKCPNPDLTVGVGRHSDISTLTLLLQDDIGGLYVRK 258

Query: 266 EIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFPE 325
                 E W HV PV  +LVINIGD+L+IMSNG YKS EH V +N S+DRIS+P+FV P+
Sbjct: 259 ---LEHEAWSHVPPVKGALVINIGDALQIMSNGRYKSIEHRVMANESNDRISVPVFVNPK 318

Query: 326 KSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
            + +VGPLPEVLA+G+K  YK V++SDY KHF  K  +GK+ +  A+I
Sbjct: 319 PNDIVGPLPEVLASGEKPVYKPVLYSDYAKHFYRKAHNGKDTIAFARI 358

[Close]

BLAST of PI0027509 vs. ExPASy Swiss-Prot
Match: G9M9M1 (Feruloyl CoA ortho-hydroxylase F6H1-2 OS=Ipomoea batatas OX=4120 GN=F6H1-2 PE=1 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 5.6e-94
Identity = 172/349 (49.28%), Postives = 249/349 (71.35%), Query Frame = 0


Query: 26  INKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSK----AEAAE 85
           +++G+G  GL++M L ALPK ++QP E+R+++   +++   DTIPVID+S+     +  +
Sbjct: 17  VHEGNGVKGLADMGLEALPKQYVQPEEERITT---STVIVDDTIPVIDLSEWGSDPKVGD 76

Query: 86  LICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGT 145
           +ICEAAEKWGFFQ+VNHGVP  ++  V  A  RFF   AEEK +  K+NS  S++V YGT
Sbjct: 77  MICEAAEKWGFFQIVNHGVPLEVLEEVKAATYRFFRLPAEEKNKHCKDNSP-SNNVRYGT 136

Query: 146 SFFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKL 205
           SF   AEKALEW D L++ +VS++EA+A WP+  +D  + ++++ D +IKR+LK LM  L
Sbjct: 137 SFTPHAEKALEWKDFLSLFYVSDEEAAALWPSACRDEALTFMRNCDAVIKRLLKSLMKGL 196

Query: 206 KANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVR 265
               E++ T +        I  NYYP CPNP+L++GV  HSD+S +T+LLQD +GGL+VR
Sbjct: 197 NVT-EIDGTKESLLMGSKRINMNYYPKCPNPELTVGVGRHSDVSTLTILLQDQIGGLYVR 256

Query: 266 REIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFP 325
           + +DS  + WVHV P+  ++VIN+GD+L+I+SNG YKS EH V +NGS++RIS+PIFV P
Sbjct: 257 K-LDS--DTWVHVPPINGAIVINVGDALQILSNGRYKSIEHRVIANGSNNRISVPIFVNP 316

Query: 326 EKSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
             + ++GPLPE+L +G+KA YK V++SDY KHF  K  DGK  ++ AKI
Sbjct: 317 RPNDIIGPLPELLESGEKAVYKNVLYSDYVKHFFRKAHDGKETVDFAKI 357

[Close]

BLAST of PI0027509 vs. ExPASy Swiss-Prot
Match: G9M9M2 (Feruloyl CoA ortho-hydroxylase F6H1-3 OS=Ipomoea batatas OX=4120 GN=F6H1-3 PE=1 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 5.6e-94
Identity = 173/349 (49.57%), Postives = 250/349 (71.63%), Query Frame = 0


Query: 26  INKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSK----AEAAE 85
           +++G+G  GL++M L++LPK +IQP E+R+++   +++   DTIPVID+S+     +  +
Sbjct: 17  VHEGNGVKGLADMGLQSLPKQYIQPAEERITT---STVIVDDTIPVIDLSEWGSDPKVGD 76

Query: 86  LICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGT 145
           LICEAAEKWGFFQ+VNHGVP  ++  V  A  RFF   AEEK +  K+NS  S++V YGT
Sbjct: 77  LICEAAEKWGFFQIVNHGVPLEVLEEVKAATYRFFRLPAEEKNKHSKDNSP-SNNVRYGT 136

Query: 146 SFFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKL 205
           SF   AEKALEW D L++ +VS++EA+A WP+  +D  + ++++ D +IKR+LK LM  L
Sbjct: 137 SFTPHAEKALEWKDFLSLFYVSDEEAAALWPSACRDEALTFMRNCDAVIKRLLKSLMKGL 196

Query: 206 KANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVR 265
               E++ T +        I  NYYP CPNP+L++GV  HSD+S +T+LLQD +GGL+VR
Sbjct: 197 NVT-EIDGTKESLLMGSKRINMNYYPKCPNPELTVGVGRHSDVSTLTILLQDQIGGLYVR 256

Query: 266 REIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFP 325
           + +DS  + WVHV P+  ++VIN+GD+L+I+SNG YKS EH V +NGS++RIS+PIFV P
Sbjct: 257 K-LDS--DTWVHVPPINGAIVINVGDALQILSNGRYKSIEHRVIANGSNNRISVPIFVNP 316

Query: 326 EKSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
             + ++GPLPE+L +G+KA YK V++SDY KHF  K  DGK  ++ AKI
Sbjct: 317 RPNDIIGPLPELLESGEKAVYKNVLYSDYVKHFFRKAHDGKETVDFAKI 357

[Close]

BLAST of PI0027509 vs. ExPASy TrEMBL
Match: A0A0A0LFH9 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G821020 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 4.8e-165
Identity = 300/366 (81.97%), Postives = 329/366 (89.89%), Query Frame = 0


Query: 1   MASMWDSNSNFKAVNEEEDHPWKEAINKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIR 60
           MASM+DS+S+ K VNEE DHPWKEAINKG+G TGLS+MRLRALPKAFIQPPEKR SSMIR
Sbjct: 1   MASMYDSDSDSKPVNEEGDHPWKEAINKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIR 60

Query: 61  A-SLASGDTIPVIDMSKAEAAELICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQA 120
           A S ASGDTIP+IDMSKAEAAELICEAAEKWGFFQV+NHGVPA LMS+VM AARRF GQA
Sbjct: 61  ASSQASGDTIPIIDMSKAEAAELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQA 120

Query: 121 AEEKRRFLKENSGCSDHVIYGTSFFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDAT 180
           AEEKRRFLKEN+ CS +V+Y TSFF EAEK LEWSD+L+M FVSE+EA AFWPA+SKDAT
Sbjct: 121 AEEKRRFLKENTSCS-NVVYMTSFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDAT 180

Query: 181 IEYLKSSDTLIKRVLKILMNKLKANIELEETNQCPTIQFNYYPICPNPDLSIGVRSHSDI 240
           IEYLKSS+TLIKRVLKILMNKL AN EL+ETNQ   IQFNYYPI P PDLS+GVRSHSD+
Sbjct: 181 IEYLKSSNTLIKRVLKILMNKLNAN-ELDETNQTRRIQFNYYPISPTPDLSVGVRSHSDV 240

Query: 241 SLVTVLLQDDVGGLHVRREIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCV 300
           SL+TVLLQDDVGGLHVRREIDS G+EWV VKPVAESLVINIGD LEIMSNGIYKS EHCV
Sbjct: 241 SLLTVLLQDDVGGLHVRREIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCV 300

Query: 301 ASNGSSDRISIPIFVFPEKSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNN 360
            SNG+ DR+SIPIFV  + S  +GPLPEVLATGKKAEYKEVVFSDY K+FL+KPLDGK+ 
Sbjct: 301 VSNGNIDRMSIPIFVGLQNSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKST 360

Query: 361 LELAKI 366
           L+LAKI
Sbjct: 361 LKLAKI 364

[Close]

BLAST of PI0027509 vs. ExPASy TrEMBL
Match: M5W6M1 (Fe2OG dioxygenase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6G006000 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 3.7e-101
Identity = 193/348 (55.46%), Postives = 251/348 (72.13%), Query Frame = 0


Query: 26  INKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSK---AEAAEL 85
           IN G+G  GLSEM L++LPK +IQP E+R+S+       S +T+P+IDMS     + AE 
Sbjct: 16  INNGNGVKGLSEMGLKSLPKQYIQPLEERISTASFGIKNSEETVPIIDMSNWDDPKVAEA 75

Query: 86  ICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGTS 145
           IC AAEKWGFFQ+VNHGVP  ++ NV +A  RFF   AEEK +  KE S CS++V +GTS
Sbjct: 76  ICSAAEKWGFFQLVNHGVPVEVLENVKEATHRFFELPAEEKSKNSKERS-CSNNVRFGTS 135

Query: 146 FFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKLK 205
           F  +AEKALEW D+L++ +VSEDEASA WP   KD  +EY+K S+ LIKR+L++LM +L 
Sbjct: 136 FSPQAEKALEWKDYLSLFYVSEDEASALWPPACKDEVLEYMKKSEILIKRLLEMLMKRLN 195

Query: 206 ANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVRR 265
              E+++  +        I  NYYPICPNP+L++GV  HSD+S +TVLLQD +GGL+VR 
Sbjct: 196 VK-EIDQEKEGLLMGSMRINLNYYPICPNPELTVGVGRHSDVSTLTVLLQDQIGGLYVRG 255

Query: 266 EIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFPE 325
             D   + W+HV PV  SLVINIGD+L+IMSNG YKS EH VA+NGS +RIS+PIFV P 
Sbjct: 256 GTDK--KTWIHVPPVKGSLVINIGDALQIMSNGRYKSIEHRVAANGSKNRISVPIFVNPR 315

Query: 326 KSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
            S ++ PLPEVLA+G+KA YK+V++SDY KHF  K  DGK+ +E AKI
Sbjct: 316 PSDLISPLPEVLASGEKAVYKQVLYSDYVKHFFRKAHDGKSTIEFAKI 359

[Close]

BLAST of PI0027509 vs. ExPASy TrEMBL
Match: A0A2K1R4B1 (Fe2OG dioxygenase domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_T170500 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 4.9e-101
Identity = 193/348 (55.46%), Postives = 253/348 (72.70%), Query Frame = 0


Query: 26  INKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSK---AEAAEL 85
           +NKG+G  GLSEM L +LPK +IQP E+R   M    + S ++IP+IDMSK    + AE 
Sbjct: 20  LNKGNGVKGLSEMGLESLPKQYIQPLEER---MCGTKIMSHESIPIIDMSKWDDPKVAEA 79

Query: 86  ICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGTS 145
           ICEAAEKWGFFQ++NHGVP  ++ NV +A  +FF   AEEKR++LKE S  S++V +GTS
Sbjct: 80  ICEAAEKWGFFQIINHGVPIEVLENVKEATHQFFRLPAEEKRKYLKEFSP-SNNVRFGTS 139

Query: 146 FFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKLK 205
           F  EAEKALEW D+L++ +VSEDEASA WPAV KD  +EY+K S+T+I+++L +LM  L 
Sbjct: 140 FSPEAEKALEWKDYLSLFYVSEDEASALWPAVCKDQVLEYMKRSETVIRKLLDVLMKNLN 199

Query: 206 ANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVRR 265
              E++ET +           NYYPICPNP+L++GV  HSD+S +T LLQDD+GGL+VR 
Sbjct: 200 VT-EIDETKESLLMGSKRTNLNYYPICPNPELTVGVGRHSDVSTLTFLLQDDIGGLYVRG 259

Query: 266 EIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFPE 325
             DS    W+HV PV+ S+VIN+GD+L+IMSNG YKS EH V +NGS++RIS+PIF+ P 
Sbjct: 260 NNDS----WIHVPPVSCSIVINVGDALQIMSNGRYKSIEHRVIANGSNNRISVPIFINPM 319

Query: 326 KSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
            S  + P PEVLA G+KA YKEV++SDY KHF  K  DGK  ++LAKI
Sbjct: 320 PSDKISPFPEVLAGGEKAVYKEVLYSDYVKHFFRKAHDGKKTIDLAKI 358

[Close]

BLAST of PI0027509 vs. ExPASy TrEMBL
Match: A0A438ELM9 (Feruloyl CoA ortho-hydroxylase 1 OS=Vitis vinifera OX=29760 GN=F6'H1_2 PE=3 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 8.3e-101
Identity = 190/348 (54.60%), Postives = 255/348 (73.28%), Query Frame = 0


Query: 26  INKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSKAE---AAEL 85
           +N+GHG  GLSEM+L ++PK FIQPPE+R+ +   + +A  + IPVIDM+  +    AEL
Sbjct: 20  VNQGHGVKGLSEMKLPSIPKQFIQPPEERIDA---SKVAREECIPVIDMAGVDDPKVAEL 79

Query: 86  ICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGTS 145
           IC+AAEKWGFFQVVNHGVP  ++ +V +A  RFF    EEK+++ KENS  S+ V +GTS
Sbjct: 80  ICDAAEKWGFFQVVNHGVPIGVLEDVKEATHRFFALPGEEKKKYSKENSN-SNSVRFGTS 139

Query: 146 FFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKLK 205
           F  +AEK+LEW D+L++ +VSEDEASA WP+V KD  +EY+K S+ +I+++L +LM +L 
Sbjct: 140 FTPQAEKSLEWKDYLSLFYVSEDEASALWPSVCKDQALEYMKRSELVIRQLLDVLMRRLN 199

Query: 206 ANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVRR 265
              E++E  +        I  NYYPICPNP+L++GV  HSD+S +TVLLQDD+GGL+V+ 
Sbjct: 200 VK-EIDEKKESLLMGSMRINLNYYPICPNPELTVGVGRHSDVSTLTVLLQDDIGGLYVKG 259

Query: 266 EIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFPE 325
             D+    WVHV P   SLVINIGD+L+IMSNG YKS EH V +NGSS+R+S+PIFV P 
Sbjct: 260 VHDT----WVHVPPRRGSLVINIGDALQIMSNGRYKSVEHRVIANGSSNRVSVPIFVNPR 319

Query: 326 KSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
            S V+GPLPEVL  G++  YK+V++SDY KHF  K  DGKN++E AKI
Sbjct: 320 PSEVIGPLPEVLEGGEEPLYKQVLYSDYVKHFFRKAHDGKNSVEFAKI 358

[Close]

BLAST of PI0027509 vs. ExPASy TrEMBL
Match: A0A4U5QC14 (Oxidoreductase family protein OS=Populus alba OX=43335 GN=D5086_0000108930 PE=3 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 8.3e-101
Identity = 191/348 (54.89%), Postives = 253/348 (72.70%), Query Frame = 0


Query: 26  INKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSK---AEAAEL 85
           +NKG+G  GLS+M L +LPK +IQP E+R   M    + S ++IP+IDMSK    + AE 
Sbjct: 20  LNKGNGVKGLSDMGLESLPKQYIQPLEER---MCGTKIMSHESIPIIDMSKWDDPKVAEA 79

Query: 86  ICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGTS 145
           ICEAAEKWGFFQ++NHGVP  ++ NV +A  +FF   AEEKR++LKE S  S++V +GTS
Sbjct: 80  ICEAAEKWGFFQIINHGVPIEVLENVKEATHQFFRLPAEEKRKYLKEFSP-SNNVRFGTS 139

Query: 146 FFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKLK 205
           F  EAEKALEW D+L++ +VSEDEASA WPAV KD  +EY+K S+ +I+++L +LM  L 
Sbjct: 140 FSPEAEKALEWKDYLSLFYVSEDEASAMWPAVCKDQVLEYMKRSEIVIRKLLDVLMKNLN 199

Query: 206 ANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVRR 265
              E++ET +           NYYPICPNP+L++GV  HSD+S +T LLQDD+GGL+VR 
Sbjct: 200 VT-EIDETKESLLMGSKRTNLNYYPICPNPELTVGVGRHSDVSTLTFLLQDDIGGLYVRG 259

Query: 266 EIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFPE 325
             DS    W+HV PV+ S+VIN+GD+L+IMSNG YKS EH V +NG+S+RIS+PIF+ P 
Sbjct: 260 NNDS----WIHVPPVSGSIVINVGDALQIMSNGRYKSIEHRVIANGNSNRISVPIFINPR 319

Query: 326 KSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
            S  + P PEVLA+G+KA YKEV++SDY KHF  K  DGK  ++LAKI
Sbjct: 320 PSDKISPFPEVLASGEKAVYKEVLYSDYVKHFFRKAHDGKKTIDLAKI 358

[Close]

BLAST of PI0027509 vs. NCBI nr
Match: XP_011652189.1 (feruloyl CoA ortho-hydroxylase 1 [Cucumis sativus] >KGN59447.1 hypothetical protein Csa_001767 [Cucumis sativus])

HSP 1 Score: 590.5 bits (1521), Expect = 9.9e-165
Identity = 300/366 (81.97%), Postives = 329/366 (89.89%), Query Frame = 0


Query: 1   MASMWDSNSNFKAVNEEEDHPWKEAINKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIR 60
           MASM+DS+S+ K VNEE DHPWKEAINKG+G TGLS+MRLRALPKAFIQPPEKR SSMIR
Sbjct: 1   MASMYDSDSDSKPVNEEGDHPWKEAINKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIR 60

Query: 61  A-SLASGDTIPVIDMSKAEAAELICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQA 120
           A S ASGDTIP+IDMSKAEAAELICEAAEKWGFFQV+NHGVPA LMS+VM AARRF GQA
Sbjct: 61  ASSQASGDTIPIIDMSKAEAAELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQA 120

Query: 121 AEEKRRFLKENSGCSDHVIYGTSFFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDAT 180
           AEEKRRFLKEN+ CS +V+Y TSFF EAEK LEWSD+L+M FVSE+EA AFWPA+SKDAT
Sbjct: 121 AEEKRRFLKENTSCS-NVVYMTSFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDAT 180

Query: 181 IEYLKSSDTLIKRVLKILMNKLKANIELEETNQCPTIQFNYYPICPNPDLSIGVRSHSDI 240
           IEYLKSS+TLIKRVLKILMNKL AN EL+ETNQ   IQFNYYPI P PDLS+GVRSHSD+
Sbjct: 181 IEYLKSSNTLIKRVLKILMNKLNAN-ELDETNQTRRIQFNYYPISPTPDLSVGVRSHSDV 240

Query: 241 SLVTVLLQDDVGGLHVRREIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCV 300
           SL+TVLLQDDVGGLHVRREIDS G+EWV VKPVAESLVINIGD LEIMSNGIYKS EHCV
Sbjct: 241 SLLTVLLQDDVGGLHVRREIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCV 300

Query: 301 ASNGSSDRISIPIFVFPEKSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNN 360
            SNG+ DR+SIPIFV  + S  +GPLPEVLATGKKAEYKEVVFSDY K+FL+KPLDGK+ 
Sbjct: 301 VSNGNIDRMSIPIFVGLQNSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKST 360

Query: 361 LELAKI 366
           L+LAKI
Sbjct: 361 LKLAKI 364

[Close]

BLAST of PI0027509 vs. NCBI nr
Match: KAF9865102.1 (hypothetical protein H0E87_024538 [Populus deltoides])

HSP 1 Score: 380.6 bits (976), Expect = 1.6e-101
Identity = 193/348 (55.46%), Postives = 254/348 (72.99%), Query Frame = 0


Query: 26  INKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSK---AEAAEL 85
           +NKG+G  GLSEM L +LPK +IQP E+R   M    + S ++IP+IDMSK    + AE 
Sbjct: 20  LNKGNGVKGLSEMGLESLPKQYIQPLEER---MCGTKIMSHESIPIIDMSKWDDPKVAEA 79

Query: 86  ICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGTS 145
           ICEAAEKWGFFQ++NHGVP  ++ NV +A  +FF   AEEKR++LKE S  S++V +GTS
Sbjct: 80  ICEAAEKWGFFQIINHGVPIEVLENVKEATHQFFRLPAEEKRKYLKEFSP-SNNVRFGTS 139

Query: 146 FFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKLK 205
           F  EAEKALEW D+L++ +VSEDEASA WPAV KD  +EY+K S+T+I+++L +LM  L 
Sbjct: 140 FSPEAEKALEWKDYLSLFYVSEDEASALWPAVCKDQVLEYMKRSETVIRKLLDVLMKNLN 199

Query: 206 ANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVRR 265
              E++ET +           NYYPICPNP+L++GV  HSD+S +T LLQDD+GGL+VR 
Sbjct: 200 VT-EIDETKESLLMGSKRTNLNYYPICPNPELTVGVGRHSDVSTLTFLLQDDIGGLYVRG 259

Query: 266 EIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFPE 325
             DS    W+HV PV+ S+VIN+GD+L+IMSNG YKS EH V +NGS++RIS+PIF+ P 
Sbjct: 260 NNDS----WIHVPPVSGSIVINVGDALQIMSNGRYKSIEHRVIANGSNNRISVPIFINPR 319

Query: 326 KSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
            S  + P PEVLA+G+KA YKEV++SDY KHF  K  DGK  ++LAKI
Sbjct: 320 PSDKISPFPEVLASGEKAVYKEVLYSDYVKHFFRKAHDGKKTIDLAKI 358

[Close]

BLAST of PI0027509 vs. NCBI nr
Match: XP_042959872.1 (feruloyl CoA ortho-hydroxylase F6H1-3-like [Carya illinoinensis] >KAG6630128.1 hypothetical protein CIPAW_14G134500 [Carya illinoinensis])

HSP 1 Score: 380.2 bits (975), Expect = 2.0e-101
Identity = 191/348 (54.89%), Postives = 256/348 (73.56%), Query Frame = 0


Query: 26  INKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSK---AEAAEL 85
           I+KG+G  GLSEM L  LP+ FIQP E+R+S    +++   ++IP+IDMS     + AE 
Sbjct: 21  ISKGNGVKGLSEMGLECLPQRFIQPLEERISF---SNIMPQESIPIIDMSNWNDPKVAES 80

Query: 86  ICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGTS 145
           IC+AAE WGFFQ++NHGVP   + NV  A   FFG  AE+K++FLKENS  S++V +GTS
Sbjct: 81  ICDAAETWGFFQIINHGVPIDALENVKDATHLFFGLPAEDKKKFLKENSP-SNNVRFGTS 140

Query: 146 FFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKLK 205
           F  +AEKALEW D+L++ +VSEDEASA WP V KD  +EY+KSS+ +IKR+L++LM  L 
Sbjct: 141 FTPQAEKALEWKDYLSLFYVSEDEASALWPPVCKDQVLEYMKSSEVVIKRLLEVLMKGLN 200

Query: 206 ANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVRR 265
            N E++ET +        I  NYYPICPNP+L++GV  HSD+S +T+LLQD++GGL+VR 
Sbjct: 201 VN-EIDETKESLLMGSKRINLNYYPICPNPELTVGVGRHSDVSTLTILLQDEIGGLYVRG 260

Query: 266 EIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFPE 325
           + DS    W+HV P++ SLVIN+GD+L+IMSNG YKS EH V +NGS +RIS+PIFV P+
Sbjct: 261 KNDS----WIHVPPISGSLVINVGDALQIMSNGRYKSIEHRVLANGSQNRISVPIFVNPK 320

Query: 326 KSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
              ++GP PEVLA G+KA Y++V++SDY KHF  K  DGKN +E AKI
Sbjct: 321 PCEIIGPFPEVLAGGEKAAYRQVLYSDYVKHFFRKAHDGKNTVEFAKI 359

[Close]

BLAST of PI0027509 vs. NCBI nr
Match: KAG6679526.1 (hypothetical protein I3842_14G136700 [Carya illinoinensis] >KAG7948178.1 hypothetical protein I3843_14G135300 [Carya illinoinensis])

HSP 1 Score: 379.4 bits (973), Expect = 3.5e-101
Identity = 190/348 (54.60%), Postives = 255/348 (73.28%), Query Frame = 0


Query: 26  INKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMS---KAEAAEL 85
           I+KG+G  GLSEM L  LP+ FIQP E+R+S    +++   ++IP+IDMS     + AE 
Sbjct: 21  ISKGNGVKGLSEMGLECLPQRFIQPLEERISF---SNIMPQESIPIIDMSNWNNPKVAES 80

Query: 86  ICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGTS 145
           +C+AAE WGFFQ++NHGVP   + NV  A   FFG  AE+K++FLKENS  S++V +GTS
Sbjct: 81  VCDAAETWGFFQIINHGVPIDALENVKDATHLFFGLPAEDKKKFLKENSP-SNNVRFGTS 140

Query: 146 FFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKLK 205
           F  +AEKALEW D+L++ +VSEDEASA WP V KD  +EY+KSS+ +IKR+L++LM  L 
Sbjct: 141 FTPQAEKALEWKDYLSLFYVSEDEASALWPPVCKDQVLEYMKSSEVVIKRLLEVLMKGLN 200

Query: 206 ANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVRR 265
            N E++ET +        I  NYYPICPNP+L++GV  HSD+S +T+LLQD++GGL+VR 
Sbjct: 201 VN-EIDETKESLLMGSKRINLNYYPICPNPELTVGVGRHSDVSTLTILLQDEIGGLYVRG 260

Query: 266 EIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFPE 325
           + DS    W+HV P+  SLVIN+GD+L+IMSNG YKS EH V +NGS +RIS+PIFV P+
Sbjct: 261 KNDS----WIHVPPICGSLVINVGDALQIMSNGRYKSIEHRVLANGSQNRISVPIFVNPK 320

Query: 326 KSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
              ++GP PEVLA G+KA Y++V++SDY KHF  K  DGKN +E AKI
Sbjct: 321 PCEIIGPFPEVLAGGEKAAYRQVLYSDYVKHFFRKAHDGKNTVEFAKI 359

[Close]

BLAST of PI0027509 vs. NCBI nr
Match: XP_040994549.1 (feruloyl CoA ortho-hydroxylase F6H1-2-like [Juglans microcarpa x Juglans regia])

HSP 1 Score: 378.3 bits (970), Expect = 7.7e-101
Identity = 189/348 (54.31%), Postives = 257/348 (73.85%), Query Frame = 0


Query: 26  INKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSK---AEAAEL 85
           I+KG+G  GLSEM L  LP+ FIQP E+R+S    +++   ++IP+IDMS     + AE 
Sbjct: 20  ISKGNGVKGLSEMGLECLPQRFIQPLEERISF---SNIMPQESIPIIDMSNWNDPKVAES 79

Query: 86  ICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGTS 145
           IC+AAE WGFFQ++NHGVP  ++ NV  A   FFG  AE+K++FLKENS  S +V +GTS
Sbjct: 80  ICDAAETWGFFQIINHGVPIEVLENVKDATHLFFGLPAEDKKKFLKENSP-SKNVRFGTS 139

Query: 146 FFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKLK 205
           F  +AEKALEW D+L++ +VSEDEASA WP+V KD  +EY+KSS+ +IKR+L++LM +L 
Sbjct: 140 FSPKAEKALEWKDYLSLFYVSEDEASASWPSVCKDQVLEYMKSSEVVIKRLLEVLMKRLN 199

Query: 206 ANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVRR 265
            N E++E  +        I  NYYPICPNP+L++GV  HSD+S +T+LLQD++GGL+VR 
Sbjct: 200 VN-EIDEMKESLLMGSKRINLNYYPICPNPELTVGVGRHSDVSTLTILLQDEIGGLYVRG 259

Query: 266 EIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFPE 325
           + DS    W+HV P++ SLVIN+GD+L+IMSNG YKS EH V +NGS +RIS+PIFV P+
Sbjct: 260 KNDS----WIHVPPISGSLVINVGDALQIMSNGRYKSIEHRVLANGSQNRISVPIFVNPK 319

Query: 326 KSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
              ++GP PE+LA G+KA Y++V++SDY KHF  K  DGKN +E AKI
Sbjct: 320 PYEIIGPFPEMLAGGEKAAYRQVLYSDYVKHFFGKAHDGKNTVEFAKI 358

[Close]

BLAST of PI0027509 vs. TAIR 10
Match: AT3G13610.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 345.9 bits (886), Expect = 4.0e-95
Identity = 178/348 (51.15%), Postives = 243/348 (69.83%), Query Frame = 0


Query: 26  INKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSKAE---AAEL 85
           + KG+G  GLSE  ++ALP+ +IQP E+RL +  +    + + IPVIDMS  +    AE 
Sbjct: 21  VYKGNGVKGLSETGIKALPEQYIQPLEERLIN--KFVNETDEAIPVIDMSNPDEDRVAEA 80

Query: 86  ICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGTS 145
           +C+AAEKWGFFQV+NHGVP  ++ +V  A  +FF    EEKR+F KENS  S  V +GTS
Sbjct: 81  VCDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNLPVEEKRKFTKENS-LSTTVRFGTS 140

Query: 146 FFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKLK 205
           F   AE+ALEW D+L++ FVSE EA  FWP + ++ T+EY+  S  +++R+L+ L   L 
Sbjct: 141 FSPLAEQALEWKDYLSLFFVSEAEAEQFWPDICRNETLEYINKSKKMVRRLLEYLGKNLN 200

Query: 206 ANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVRR 265
              EL+ET +        +  NYYPICPNPDL++GV  HSD+S +T+LLQD +GGLHV R
Sbjct: 201 VK-ELDETKESLFMGSIRVNLNYYPICPNPDLTVGVGRHSDVSSLTILLQDQIGGLHV-R 260

Query: 266 EIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFPE 325
            + SG   WVHV PVA S VINIGD+++IMSNG+YKS EH V +NG ++RIS+PIFV P+
Sbjct: 261 SLASG--NWVHVPPVAGSFVINIGDAMQIMSNGLYKSVEHRVLANGYNNRISVPIFVNPK 320

Query: 326 KSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
             SV+GPLPEV+A G++  Y++V++SDY K+F  K  DGK  ++ AKI
Sbjct: 321 PESVIGPLPEVIANGEEPIYRDVLYSDYVKYFFRKAHDGKKTVDYAKI 361

[Close]

BLAST of PI0027509 vs. TAIR 10
Match: AT1G55290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 332.4 bits (851), Expect = 4.5e-91
Identity = 169/348 (48.56%), Postives = 238/348 (68.39%), Query Frame = 0


Query: 26  INKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSKAE---AAEL 85
           +++G+G  GLSE  ++ LP  +IQP E+RL +       S ++IPVID+S  +    ++ 
Sbjct: 20  VHEGNGVKGLSETGIKVLPDQYIQPFEERLIN-FHVKEDSDESIPVIDISNLDEKSVSKA 79

Query: 86  ICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGTS 145
           +C+AAE+WGFFQV+NHGV   ++ N+  A  RFFG   EEKR+F +E S  S +V +GTS
Sbjct: 80  VCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPVEEKRKFSREKS-LSTNVRFGTS 139

Query: 146 FFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKLK 205
           F   AEKALEW D+L++ FVSE EAS  WP   +  T+EY+  +  L+K++L+ L   L 
Sbjct: 140 FSPHAEKALEWKDYLSLFFVSEAEASQLWPDSCRSETLEYMNETKPLVKKLLRFLGENLN 199

Query: 206 ANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVRR 265
              EL++T +        I  NYYPICPNP+L++GV  HSD+S +T+LLQD++GGLHV R
Sbjct: 200 VK-ELDKTKESFFMGSTRINLNYYPICPNPELTVGVGRHSDVSSLTILLQDEIGGLHV-R 259

Query: 266 EIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFPE 325
            + +G   WVHV P++ SLVINIGD+++IMSNG YKS EH V +NGS +RIS+PIFV P+
Sbjct: 260 SLTTG--RWVHVPPISGSLVINIGDAMQIMSNGRYKSVEHRVLANGSYNRISVPIFVSPK 319

Query: 326 KSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
             SV+GPL EV+  G+K  YK+++++DY KHF  K  DGK  ++ A I
Sbjct: 320 PESVIGPLLEVIENGEKPVYKDILYTDYVKHFFRKAHDGKKTIDFANI 361

[Close]

BLAST of PI0027509 vs. TAIR 10
Match: AT3G12900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.7e-75
Identity = 152/358 (42.46%), Postives = 221/358 (61.73%), Query Frame = 0


Query: 16  EEEDHPWKEAINKGHGATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMS 75
           E++   +   + +G+G  G+ +  L ++P+ F+QP  +R+ +  + +L    T P ID+S
Sbjct: 6   EDQTTLFNFVVREGNGVKGMIDSGLSSVPRPFVQPLSERIPT--QKALTCEATQP-IDLS 65

Query: 76  K------AEAAELICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKE 135
                   E A+ I EAAE  GFFQVVNHGV   L+  +  +A  FF QA EEK  +LKE
Sbjct: 66  NLDGPQHKEVAKQIVEAAETLGFFQVVNHGVSVELLELLKSSAHEFFAQAPEEKSMYLKE 125

Query: 136 NSGCSDHVIYGTSFFIEAEKALEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTL 195
            S  S  V YGTSF  + EKA+EW D+++M + ++ EA   WP   ++  +E+L SS  +
Sbjct: 126 VSP-SKLVKYGTSFVPDKEKAIEWKDYVSMLYTNDSEALQHWPQPCREVALEFLNSSMEM 185

Query: 196 IKRVLKILMNKLKANIELEETN---QCPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLL 255
           +K V+ ILM  +   +E E+ N       +  NYYP CP+P+L++GV  HSD+ ++TVLL
Sbjct: 186 VKNVVNILMENVGVTLEEEKMNGLMGTKMVNMNYYPTCPSPELTVGVGRHSDMGMLTVLL 245

Query: 256 QDDVGGLHVRREIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSD 315
           QD +GGL+V+  +D+G  EW  + PV  +LVINIGD+L+I+SNG YKSAEH V +     
Sbjct: 246 QDGIGGLYVK--LDNG--EWAEIPPVHGALVINIGDTLQILSNGKYKSAEHRVRTTNIGS 305

Query: 316 RISIPIFVFPEKSSVVGPLPEVLATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAK 365
           R+S+PIF  P  S  VGPLPEV+     A YKE +F DY  +F  +P DGK +L+ A+
Sbjct: 306 RVSVPIFTAPNPSQKVGPLPEVVKRDGVARYKEFLFQDYMNNFFGQPHDGKKSLDFAR 355

[Close]

BLAST of PI0027509 vs. TAIR 10
Match: AT5G12270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 4.6e-67
Identity = 143/353 (40.51%), Postives = 209/353 (59.21%), Query Frame = 0


Query: 26  INKGHGATGLSE-MRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMS---KAEAAE 85
           +N+ +G  GL + + L  LP  +IQPP++R +S     +  G  +PVID+S   +   A 
Sbjct: 15  VNQKNGVKGLVDFLTLTTLPSPYIQPPQERFTS---DKILLGSPVPVIDVSNWNEPHVAR 74

Query: 86  LICEAAEKWGFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGT 145
            IC AA K G FQ+VNHG+  A    V+ AAR FF   AEE+RR+ +  S  S+     T
Sbjct: 75  EICHAASKLGLFQIVNHGIAPAEFKGVIAAARGFFELPAEERRRYWR-GSSVSETAWLTT 134

Query: 146 SFFIEAEKALEWSDHLTMPFVSE-DEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNK 205
           SF    E  LEW D L   ++ +  + +A WP+V K+  I++ K    + +R+L IL+N 
Sbjct: 135 SFNPCIESVLEWRDFLKFEYLPQRHDFAATWPSVCKEQVIDHFKRIKPITERILNILINN 194

Query: 206 LKANIELEETNQ-----CPTIQFNYYPICPNPDLSIGVRSHSDISLVTVLLQDD--VGGL 265
           L  N  ++E+N+        + FNYYP CP P L+IG   HSDI+ +T+LLQ+D  +  L
Sbjct: 195 L--NTIIDESNKETLMGTMRMNFNYYPKCPEPSLAIGTGRHSDINTLTLLLQEDGVLSSL 254

Query: 266 HVRREIDSGGEEWVHVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIF 325
           + R   D  G++W+HV P+  ++V+NIGD L+I+SN  Y+S EHCV  N    R+SIP+F
Sbjct: 255 YARATED--GDKWIHVPPIPGAIVVNIGDVLQILSNDRYRSVEHCVVVNKYCSRVSIPVF 314

Query: 326 VFPEKSSVVGPLPEVL-ATGKKAEYKEVVFSDYFKHFLSKPLDGKNNLELAKI 366
             P   SV+ PLPEVL    + A Y+++V+SDY K F  +P DGK  +E  K+
Sbjct: 315 CGPVHDSVIEPLPEVLDKNNEMARYRKIVYSDYLKFFFGRPHDGKKTIESIKL 359

[Close]

BLAST of PI0027509 vs. TAIR 10
Match: AT1G03400.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 189.1 bits (479), Expect = 6.2e-48
Identity = 117/332 (35.24%), Postives = 175/332 (52.71%), Query Frame = 0


Query: 31  GATGLSEMRLRALPKAFIQPPEKRLSSMIRASLASGDTIPVIDMSKAEAAELICEAAEKW 90
           G  GL +  +  +P  F   P   L+S+         TIP +D+  A   E I EAAEKW
Sbjct: 19  GVKGLVDSGITEIPALFRATP-ATLASLKSPPPPKHLTIPTVDLKGASVVEKIGEAAEKW 78

Query: 91  GFFQVVNHGVPAALMSNVMQAARRFFGQAAEEKRRFLKENSGCSDHVIYGTSFFIEAEKA 150
           G F +VNHG+P  ++  ++Q  R F  Q  E K+RF   +   +  V+Y ++  ++  +A
Sbjct: 79  GLFHLVNHGIPVEVLERMIQGIRGFHEQEPEAKKRFYSRDH--TRDVLYFSNHDLQNSEA 138

Query: 151 LEWSDHLTMPFVSEDEASAFWPAVSKDATIEYLKSSDTLIKRVLKILMNKLKANIELEET 210
             W D L      E       PAV  +  +EY K   +L +R+ ++L   L  N    + 
Sbjct: 139 ASWRDTLGCYTAPEPPRLEDLPAVCGEIMLEYSKEIMSLGERLFELLSEALGLNSHHLKD 198

Query: 211 NQCPTIQF---NYYPICPNPDLSIGVRSHSDISLVTVLLQDDVGGLHVRREIDSGGEEWV 270
             C   Q+    +YP CP PDL+IG+  H+DIS +TVLLQD+VGGL V  E     + W+
Sbjct: 199 MDCAKSQYMVGQHYPPCPQPDLTIGINKHTDISFLTVLLQDNVGGLQVFHE-----QYWI 258

Query: 271 HVKPVAESLVINIGDSLEIMSNGIYKSAEHCVASNGSSDRISIPIFVFP----EKSSVVG 330
            V PV  +LVINIGD L++++N  + SAEH V +NGSS+  +    VF       S V G
Sbjct: 259 DVTPVPGALVINIGDFLQLITNDKFISAEHRVIANGSSEPRTSVAIVFSTFMRAYSRVYG 318

Query: 331 PLPEVLATGKKAEYKEVVFSDYFKHFLSKPLD 356
           P+ ++L+    A+Y++   +++   F SK LD
Sbjct: 319 PIKDLLSAENPAKYRDCTLTEFSTIFSSKTLD 342

The following BLAST results are available for this feature:

BLAST of PI0027509 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G9M9M3	3.9e-95	51.15	Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-2-1 OS=Ipomo...	[more]
G9M9M4	5.0e-95	51.15	Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-1-1 OS=Ipomo...	[more]
G9M9M5	2.5e-94	50.86	Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-1-8 OS=Ipomo...	[more]
G9M9M1	5.6e-94	49.28	Feruloyl CoA ortho-hydroxylase F6H1-2 OS=Ipomoea batatas OX=4120 GN=F6H1-2 PE=1 ...	[more]
G9M9M2	5.6e-94	49.57	Feruloyl CoA ortho-hydroxylase F6H1-3 OS=Ipomoea batatas OX=4120 GN=F6H1-3 PE=1 ...	[more]

BLAST of PI0027509 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFH9	4.8e-165	81.97	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
M5W6M1	3.7e-101	55.46	Fe2OG dioxygenase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6...	[more]
A0A2K1R4B1	4.9e-101	55.46	Fe2OG dioxygenase domain-containing protein OS=Populus trichocarpa OX=3694 GN=PO...	[more]
A0A438ELM9	8.3e-101	54.60	Feruloyl CoA ortho-hydroxylase 1 OS=Vitis vinifera OX=29760 GN=F6'H1_2 PE=3 SV=1	[more]
A0A4U5QC14	8.3e-101	54.89	Oxidoreductase family protein OS=Populus alba OX=43335 GN=D5086_0000108930 PE=3 ...	[more]

BLAST of PI0027509 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011652189.1	9.9e-165	81.97	feruloyl CoA ortho-hydroxylase 1 [Cucumis sativus] >KGN59447.1 hypothetical prot...	[more]
KAF9865102.1	1.6e-101	55.46	hypothetical protein H0E87_024538 [Populus deltoides]	[more]
XP_042959872.1	2.0e-101	54.89	feruloyl CoA ortho-hydroxylase F6H1-3-like [Carya illinoinensis] >KAG6630128.1 h...	[more]
KAG6679526.1	3.5e-101	54.60	hypothetical protein I3842_14G136700 [Carya illinoinensis] >KAG7948178.1 hypothe...	[more]
XP_040994549.1	7.7e-101	54.31	feruloyl CoA ortho-hydroxylase F6H1-2-like [Juglans microcarpa x Juglans regia]	[more]

BLAST of PI0027509 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13610.1	4.0e-95	51.15	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G55290.1	4.5e-91	48.56	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G12900.1	2.7e-75	42.46	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G12270.1	4.6e-67	40.51	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G03400.1	6.2e-48	35.24	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 218..316 e-value: 1.0E-24 score: 86.9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 69..162 e-value: 2.9E-18 score: 66.7
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 23..348 e-value: 2.0E-85 score: 289.2
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 26..357
None	No IPR available	PANTHER	PTHR10209:SF764	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE-RELATED	coord: 26..357
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 33..357
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 209..316 score: 12.161335

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0027509.1	PI0027509.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity