PI0027061 (gene) Melon (PI 482460) v1

Overview
NamePI0027061
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionIntegrase catalytic domain-containing protein
Locationchr01: 13622342 .. 13622631 (+)
RNA-Seq ExpressionPI0027061
SyntenyPI0027061
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTGACGCGAGTGATGTTGCGGTAGTGGCTATGCTGGGGCAAAAGAGAAACAAAGTGATCCACCCTATATATTACGCGAGCAAGACTCTTACAGACGCTCAAGAAAACTACACTACCACGGAAAAGGAGCTGCTTACGATAGTATTTGCGGTAGAGAAGTTCAGGAGTTACATAATTGGCTCCAAAGTTACGGTACATTCTGATCATTCTGCGATCAGATTCCTCATGGCGAAGAAGGAGGCAAAACCGCGGTTAATCAGATGGGTGTTATTACTCCAAGAATTTGA

mRNA sequence

ATGTGTGACGCGAGTGATGTTGCGGTAGTGGCTATGCTGGGGCAAAAGAGAAACAAAGTGATCCACCCTATATATTACGCGAGCAAGACTCTTACAGACGCTCAAGAAAACTACACTACCACGGAAAAGGAGCTGCTTACGATAGTATTTGCGGTAGAGAAGTTCAGGAGTTACATAATTGGCTCCAAAATTCCTCATGGCGAAGAAGGAGGCAAAACCGCGGTTAATCAGATGGGTGTTATTACTCCAAGAATTTGA

Coding sequence (CDS)

ATGTGTGACGCGAGTGATGTTGCGGTAGTGGCTATGCTGGGGCAAAAGAGAAACAAAGTGATCCACCCTATATATTACGCGAGCAAGACTCTTACAGACGCTCAAGAAAACTACACTACCACGGAAAAGGAGCTGCTTACGATAGTATTTGCGGTAGAGAAGTTCAGGAGTTACATAATTGGCTCCAAAATTCCTCATGGCGAAGAAGGAGGCAAAACCGCGGTTAATCAGATGGGTGTTATTACTCCAAGAATTTGA

Protein sequence

MCDASDVAVVAMLGQKRNKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYIIGSKIPHGEEGGKTAVNQMGVITPRI
Homology
BLAST of PI0027061 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 9.2e-08
Identity = 30/59 (50.85%), Postives = 41/59 (69.49%), Query Frame = 0

Query: 3   DASDVAVVAMLGQKRNKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYIIG 62
           DASDVA+ A+L Q      HP+ Y S+TL + + NY+T EKELL IV+A + FR Y++G
Sbjct: 514 DASDVALGAVLSQDG----HPLSYISRTLNEHEINYSTIEKELLAIVWATKTFRHYLLG 568

BLAST of PI0027061 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.3e-06
Identity = 28/59 (47.46%), Postives = 40/59 (67.80%), Query Frame = 0

Query: 3   DASDVAVVAMLGQKRNKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYIIG 62
           DAS++A+ A+L Q      HPI + S+TL D + NY+  EKELL IV+A + FR Y++G
Sbjct: 513 DASNLALGAVLSQNG----HPISFISRTLNDHELNYSAIEKELLAIVWATKTFRHYLLG 567

BLAST of PI0027061 vs. ExPASy Swiss-Prot
Match: Q8I7P9 (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 6.6e-06
Identity = 24/60 (40.00%), Postives = 39/60 (65.00%), Query Frame = 0

Query: 3   DASDVAVVAMLGQKRNKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYIIGS 62
           DAS+ A+ A+L Q       PI Y S++L   +ENY T EKE+L I+++++  R+Y+ G+
Sbjct: 440 DASNWAIGAVLSQDDQGRDRPIAYISRSLNKTEENYATIEKEMLAIIWSLDNLRAYLYGA 499

BLAST of PI0027061 vs. ExPASy Swiss-Prot
Match: Q9UR07 (Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-11 PE=3 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.6e-04
Identity = 24/58 (41.38%), Postives = 40/58 (68.97%), Query Frame = 0

Query: 3   DASDVAVVAMLGQKR-NKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYI 60
           DASDVAV A+L QK  +   +P+ Y S  ++ AQ NY+ ++KE+L I+ +++ +R Y+
Sbjct: 713 DASDVAVGAVLSQKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWRHYL 770

BLAST of PI0027061 vs. ExPASy Swiss-Prot
Match: P0CT41 (Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-12 PE=3 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.6e-04
Identity = 24/58 (41.38%), Postives = 40/58 (68.97%), Query Frame = 0

Query: 3   DASDVAVVAMLGQKR-NKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYI 60
           DASDVAV A+L QK  +   +P+ Y S  ++ AQ NY+ ++KE+L I+ +++ +R Y+
Sbjct: 713 DASDVAVGAVLSQKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWRHYL 770

BLAST of PI0027061 vs. ExPASy TrEMBL
Match: A0A6J1DHH9 (LOW QUALITY PROTEIN: uncharacterized protein LOC111020110 OS=Momordica charantia OX=3673 GN=LOC111020110 PE=4 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.4e-19
Identity = 47/64 (73.44%), Postives = 58/64 (90.62%), Query Frame = 0

Query: 1   MCDASDVAVVAMLGQKRNKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYII 60
           MCDASD A+ AMLGQ++NK++HP+YYASKTLT AQ NYTTTEKELL +VFA +KFRSY+I
Sbjct: 352 MCDASDYAIGAMLGQRKNKILHPVYYASKTLTGAQLNYTTTEKELLAVVFAFDKFRSYLI 411

Query: 61  GSKI 65
           G+K+
Sbjct: 412 GTKV 415

BLAST of PI0027061 vs. ExPASy TrEMBL
Match: A0A6J1E110 (uncharacterized protein LOC111025424 OS=Momordica charantia OX=3673 GN=LOC111025424 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 9.2e-19
Identity = 46/63 (73.02%), Postives = 56/63 (88.89%), Query Frame = 0

Query: 2   CDASDVAVVAMLGQKRNKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYIIG 61
           CDASD A+ AMLGQ++NK++HP+YYASKTLT AQ NYTTTEKELL +VFA  KFRSY+IG
Sbjct: 574 CDASDYAIGAMLGQRKNKILHPVYYASKTLTGAQLNYTTTEKELLAVVFAFNKFRSYLIG 633

Query: 62  SKI 65
           +K+
Sbjct: 634 TKV 636

BLAST of PI0027061 vs. ExPASy TrEMBL
Match: A0A6J1DCL7 (LOW QUALITY PROTEIN: uncharacterized protein LOC111019515 OS=Momordica charantia OX=3673 GN=LOC111019515 PE=4 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.2e-18
Identity = 46/64 (71.88%), Postives = 56/64 (87.50%), Query Frame = 0

Query: 1   MCDASDVAVVAMLGQKRNKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYII 60
           MCDASD A+ AMLGQ++NK++HP+YYASKTLT AQ NYTTTEKELL IVF  +KFR Y+I
Sbjct: 433 MCDASDYAIGAMLGQRKNKILHPVYYASKTLTGAQLNYTTTEKELLAIVFTFDKFRXYLI 492

Query: 61  GSKI 65
           G+K+
Sbjct: 493 GTKV 496

BLAST of PI0027061 vs. ExPASy TrEMBL
Match: A0A6J1E3L7 (uncharacterized protein LOC111025754 OS=Momordica charantia OX=3673 GN=LOC111025754 PE=4 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.2e-18
Identity = 46/64 (71.88%), Postives = 57/64 (89.06%), Query Frame = 0

Query: 1   MCDASDVAVVAMLGQKRNKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYII 60
           MCDASD A+ AMLGQ++NK++HP+YYASKTLT AQ NYTTTEKELL +VFA +K RSY+I
Sbjct: 102 MCDASDYAIGAMLGQRKNKILHPVYYASKTLTXAQLNYTTTEKELLAVVFAFDKSRSYLI 161

Query: 61  GSKI 65
           G+K+
Sbjct: 162 GTKV 165

BLAST of PI0027061 vs. ExPASy TrEMBL
Match: A0A5D3CVE7 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold637G00800 PE=4 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.6e-18
Identity = 47/64 (73.44%), Postives = 56/64 (87.50%), Query Frame = 0

Query: 1   MCDASDVAVVAMLGQKRNKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYII 60
           M DASDVA+  MLGQK+ KVIHPIYY SKTL +A+ENYTTTEK+ L +VFA+EKFRSYI+
Sbjct: 123 MYDASDVALRTMLGQKKEKVIHPIYYVSKTLNEAKENYTTTEKQFLVVVFAIEKFRSYIV 182

Query: 61  GSKI 65
           GSK+
Sbjct: 183 GSKV 186

BLAST of PI0027061 vs. NCBI nr
Match: XP_022152366.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111020110 [Momordica charantia])

HSP 1 Score: 105.1 bits (261), Expect = 2.9e-19
Identity = 47/64 (73.44%), Postives = 58/64 (90.62%), Query Frame = 0

Query: 1   MCDASDVAVVAMLGQKRNKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYII 60
           MCDASD A+ AMLGQ++NK++HP+YYASKTLT AQ NYTTTEKELL +VFA +KFRSY+I
Sbjct: 352 MCDASDYAIGAMLGQRKNKILHPVYYASKTLTGAQLNYTTTEKELLAVVFAFDKFRSYLI 411

Query: 61  GSKI 65
           G+K+
Sbjct: 412 GTKV 415

BLAST of PI0027061 vs. NCBI nr
Match: XP_012838027.1 (PREDICTED: uncharacterized protein LOC105958568 [Erythranthe guttata])

HSP 1 Score: 102.8 bits (255), Expect = 1.5e-18
Identity = 49/64 (76.56%), Postives = 56/64 (87.50%), Query Frame = 0

Query: 1   MCDASDVAVVAMLGQKRNKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYII 60
           MCDASD AV A+LGQ++NK+ H IYYASKTL DAQ NYTTTEKELL IVFA EKFRSY+I
Sbjct: 773 MCDASDFAVGAVLGQRKNKIFHSIYYASKTLNDAQLNYTTTEKELLAIVFAFEKFRSYLI 832

Query: 61  GSKI 65
           G+K+
Sbjct: 833 GTKV 836

BLAST of PI0027061 vs. NCBI nr
Match: XP_012832904.1 (PREDICTED: uncharacterized protein LOC105953771 [Erythranthe guttata])

HSP 1 Score: 102.4 bits (254), Expect = 1.9e-18
Identity = 48/64 (75.00%), Postives = 56/64 (87.50%), Query Frame = 0

Query: 1   MCDASDVAVVAMLGQKRNKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYII 60
           MCDASD AV A+LGQ++NK+ H IYYASKTL DAQ NYTTTEKELL +VFA EKFRSY+I
Sbjct: 774 MCDASDFAVGAVLGQRKNKIFHSIYYASKTLNDAQLNYTTTEKELLAVVFAFEKFRSYLI 833

Query: 61  GSKI 65
           G+K+
Sbjct: 834 GTKV 837

BLAST of PI0027061 vs. NCBI nr
Match: XP_022158979.1 (uncharacterized protein LOC111025424 [Momordica charantia])

HSP 1 Score: 102.4 bits (254), Expect = 1.9e-18
Identity = 46/63 (73.02%), Postives = 56/63 (88.89%), Query Frame = 0

Query: 2   CDASDVAVVAMLGQKRNKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYIIG 61
           CDASD A+ AMLGQ++NK++HP+YYASKTLT AQ NYTTTEKELL +VFA  KFRSY+IG
Sbjct: 574 CDASDYAIGAMLGQRKNKILHPVYYASKTLTGAQLNYTTTEKELLAVVFAFNKFRSYLIG 633

Query: 62  SKI 65
           +K+
Sbjct: 634 TKV 636

BLAST of PI0027061 vs. NCBI nr
Match: XP_012837475.1 (PREDICTED: uncharacterized protein LOC105958021 [Erythranthe guttata])

HSP 1 Score: 102.4 bits (254), Expect = 1.9e-18
Identity = 48/64 (75.00%), Postives = 56/64 (87.50%), Query Frame = 0

Query: 1   MCDASDVAVVAMLGQKRNKVIHPIYYASKTLTDAQENYTTTEKELLTIVFAVEKFRSYII 60
           MCDASD AV A+LGQ++NK+ H IYYASKTL DAQ NYTTTEKELL +VFA EKFRSY+I
Sbjct: 773 MCDASDFAVGAVLGQRKNKIFHSIYYASKTLNDAQLNYTTTEKELLAVVFAFEKFRSYLI 832

Query: 61  GSKI 65
           G+K+
Sbjct: 833 GTKV 836

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P043239.2e-0850.85Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast... [more]
P208251.3e-0647.46Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste... [more]
Q8I7P96.6e-0640.00Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogast... [more]
Q9UR071.6e-0441.38Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
P0CT411.6e-0441.38Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
Match NameE-valueIdentityDescription
A0A6J1DHH91.4e-1973.44LOW QUALITY PROTEIN: uncharacterized protein LOC111020110 OS=Momordica charantia... [more]
A0A6J1E1109.2e-1973.02uncharacterized protein LOC111025424 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1DCL71.2e-1871.88LOW QUALITY PROTEIN: uncharacterized protein LOC111019515 OS=Momordica charantia... [more]
A0A6J1E3L71.2e-1871.88uncharacterized protein LOC111025754 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A5D3CVE71.6e-1873.44Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
XP_022152366.12.9e-1973.44LOW QUALITY PROTEIN: uncharacterized protein LOC111020110 [Momordica charantia][more]
XP_012838027.11.5e-1876.56PREDICTED: uncharacterized protein LOC105958568 [Erythranthe guttata][more]
XP_012832904.11.9e-1875.00PREDICTED: uncharacterized protein LOC105953771 [Erythranthe guttata][more]
XP_022158979.11.9e-1873.02uncharacterized protein LOC111025424 [Momordica charantia][more]
XP_012837475.11.9e-1875.00PREDICTED: uncharacterized protein LOC105958021 [Erythranthe guttata][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR041577Reverse transcriptase/retrotransposon-derived protein, RNase H-like domainPFAMPF17919RT_RNaseH_2coord: 2..64
e-value: 3.0E-21
score: 75.3
NoneNo IPR availableGENE3D3.10.20.370coord: 1..65
e-value: 1.9E-6
score: 29.8
NoneNo IPR availablePANTHERPTHR24559TRANSPOSON TY3-I GAG-POL POLYPROTEINcoord: 1..61
NoneNo IPR availablePANTHERPTHR24559:SF324TRANSPOSON TY3-I GAG-POL POLYPROTEIN-LIKE PROTEINcoord: 1..61
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 1..64

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0027061.1PI0027061.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
molecular_function GO:0003676 nucleic acid binding