PI0026329 (gene) Melon (PI 482460) v1

Overview
NamePI0026329
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionMannan endo-1,4-beta-mannosidase
Locationchr10: 18091086 .. 18093743 (-)
RNA-Seq ExpressionPI0026329
SyntenyPI0026329
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACAGGTCATCCATAAGACCTATTGCACTTTGTCTTTGAAGCTTCATGTTGCTAATTTCCAACCAAACAAGCAAACCCAATTCTCAGTGGCTACACAAACAGTAACTTCAAAGACTTGTATAACTCAATTCTATTTTGCCTTGTAAGTTCATCCATCAATTCCCCAATTTTCCAATTATAAAGGAAAAAAAATCCCACCCACAAGTTTAATTTGAAGTAATGAAGGGGTTGATTCTTTTGGTGTGGTGTGTTGCGGCCTCGGCGGCGGTGGGGCTGCCGTTACACACTGATTCACGGTGGATCGTGGACGGAGAAGGGGAAAGAGTGAAACTAAGATGTGTAAATTGGGTGTCGCATTTGGAGGCGGTGGTGGCGGAGGGGCTTTCGAAGCAGCCGATTGAGGAGATTTCGAACAGAATTGAGTGGTTGGGATTCAACTGCGTCAGACTCACTTGGCCACTGTTTTTAGCCACAAATGAGTCTTTGAATTCCCTCACCGTCCGACAGTCTTTCCAGCGCCTCGGCCTGGCCGAAGCCATCGCCGGAATCCAAGCTAACAACCCCTTCATCATCGATCTTCCTCTCTTGAAAGCTTTTGAGGTAAAATTATTTGTTAATGTTAACCAATACTTTTTTTCCTCCAAATACAAACTAAGATTCCAGTTTATACTAGGGGAGTTGTCTAATTATAATAGTTTGTATATGAATGCAGATTATGGTAAAAACTACACAGAGAGATTATAATTAGAGAATAGTAAACATTAAACACGGTTACAAAAAGAGAAAGTTGAAATATTAATACTAGTTAATCGTAAGTTGTAAGTTAGAAACATCGACTATTATCACTGAAGTGGACTACACTAGAAGTTAAGACCTAAACACCTCTTAAAACTAAATCAAACCAAACCAGTAGATTGATTTGAGTTGATTTGGTTTGGTTGTTCGACTTTTCTGACTTTTTGTTGCACTCTTAGTTTGGATATGGAGGTGAAATGTGGGTTTGATTATTGTCAGGCAGTGGTTGGAAAATTGGGGGAGGGGAAATTAATGGTGATATTGGACAATCACATAAGCAAGCCAGGGTGGTGCTGTAGTAACTTCGACGGAAATGGCTTCTTTGGTGATCAATATTTCAACCCTGACTTATGGATTAAAGGCCTCACTCGAATGGCTACCATGTTTAATGGTGTCAACCATGTTGTTGCTATGAGCCTTAGGAATGAGCTTCGTGGCCCAAAACAAAATGTCAACGACTGGTACAGGTAATCTCTTCATTATTAAATTCAAAATCCACTCTTATTCCATACAAATGTTATAAACATCAAAATTACATAAAGGTACATGCAAAGAGGAGCAGAAGCAGTTCATTCAGCAAACCCAGACATTCTTATAATCCTCTCAGGACTTAGCTTTGACAGAGACTTATCTTTTCTCAAAAACCAACCCATAAACCTCACATTCACATCCAAAACGGTATATGAAGTTCATTGGTATGCTTTTTCAGATGGATCTTCATGGGAATCAGGAAACTCAAACCAAGTATGTGGAAGAACAACTAACAACTTAATGAAAATGTCTGGATTTCTATTACAACAAGGGTTCCCTCTGTTCATAAGTGAATTTGGAATTGACCAAAGAGGAACAAATGTAAATGACAACAGATATTTGAGCTGTTTTTTAGCTGTAGCTGCTGAATTTGACCTTGACTGGGCACTTTGGACACTTGTTGGAAGCTATTATTTGAGAGAAGGGGTTGTTGGTTTGAATGAATTTTATGGAATTCTTGATTGGAATTGGTGTAATGTTAGAAATTCAACTTTCCTTCAAAGGATTTCTGTTCTTCAATCTCCATTTCAAGGGCCAGGATTAGCAGAAAGAAGGGAGTATAATGTGATTTTTCATCCATTAAGTGGGCTTTGTGTGGTGAGAAAATCATTATTAGATCCATTAAGATTGGGCCCATGTGTTGATTCAGATGCTTGGTATTATACTCCACAAAAGTTTCTAACTCTAAAAGGGACTTATTTTTGTATACAAGCAGATGAAATTGGGAAGCAAGCTAAATTGGGGATTATATGTACTGTCTCTAATGCTAAATGGGATATGATTTCTGATTCAAAATTGCATCTTTCTTCAAAGTCTAGTAATGGATCCTTGGTTTGTTTGGACGTAGATTCGAGTACAAATGAAATTGTGACCAATTCTTGTAAGTGTTTGAGTCGGGACTCCTCGTGCGACCCGAGTAGCCAGTGGTTTAAGCTCGTTAATAGTACGAGAAGTTTGGGCAGGGGAAGGTCGATGGTTAACATGGTTGGTTCATCTTTGGCTAATGTTGTGACCAAATTTGTGGAGTTATGAAAAATAATGGAGGAAAAAAAAAAAAAAAGATAATAGAAAAATGCCCCCATGCAGGATCGAACTACAGACCTTCAGTTTACAAGACTGACGCTCTACCACTGAGCTATAGGGGCTGTTGATGTTAACTACGTGTGATTTAGTTACTAATTTGTTTATTAAATTATTAACGTTTACTAACTTAAAAGGACTAATAATTTCAATCTATAAACTCTTGGTATGATTTAAAGGAAAATAATTAACTGCTTCTTCGTATTTATGTGTCATAGGGTATTATTAATTCAAGACTAGTTGCAATTTTAA

mRNA sequence

CACAGGTCATCCATAAGACCTATTGCACTTTGTCTTTGAAGCTTCATGTTGCTAATTTCCAACCAAACAAGCAAACCCAATTCTCAGTGGCTACACAAACAGTAACTTCAAAGACTTGTATAACTCAATTCTATTTTGCCTTGTAAGTTCATCCATCAATTCCCCAATTTTCCAATTATAAAGGAAAAAAAATCCCACCCACAAGTTTAATTTGAAGTAATGAAGGGGTTGATTCTTTTGGTGTGGTGTGTTGCGGCCTCGGCGGCGGTGGGGCTGCCGTTACACACTGATTCACGGTGGATCGTGGACGGAGAAGGGGAAAGAGTGAAACTAAGATGTGTAAATTGGGTGTCGCATTTGGAGGCGGTGGTGGCGGAGGGGCTTTCGAAGCAGCCGATTGAGGAGATTTCGAACAGAATTGAGTGGTTGGGATTCAACTGCGTCAGACTCACTTGGCCACTGTTTTTAGCCACAAATGAGTCTTTGAATTCCCTCACCGTCCGACAGTCTTTCCAGCGCCTCGGCCTGGCCGAAGCCATCGCCGGAATCCAAGCTAACAACCCCTTCATCATCGATCTTCCTCTCTTGAAAGCTTTTGAGGCAGTGGTTGGAAAATTGGGGGAGGGGAAATTAATGGTGATATTGGACAATCACATAAGCAAGCCAGGGTGGTGCTGTAGTAACTTCGACGGAAATGGCTTCTTTGGTGATCAATATTTCAACCCTGACTTATGGATTAAAGGCCTCACTCGAATGGCTACCATGTTTAATGGTGTCAACCATGTTGTTGCTATGAGCCTTAGGAATGAGCTTCGTGGCCCAAAACAAAATGTCAACGACTGGTACAGGTACATGCAAAGAGGAGCAGAAGCAGTTCATTCAGCAAACCCAGACATTCTTATAATCCTCTCAGGACTTAGCTTTGACAGAGACTTATCTTTTCTCAAAAACCAACCCATAAACCTCACATTCACATCCAAAACGGTATATGAAGTTCATTGGTATGCTTTTTCAGATGGATCTTCATGGGAATCAGGAAACTCAAACCAAGTATGTGGAAGAACAACTAACAACTTAATGAAAATGTCTGGATTTCTATTACAACAAGGGTTCCCTCTGTTCATAAGTGAATTTGGAATTGACCAAAGAGGAACAAATGTAAATGACAACAGATATTTGAGCTGTTTTTTAGCTGTAGCTGCTGAATTTGACCTTGACTGGGCACTTTGGACACTTGTTGGAAGCTATTATTTGAGAGAAGGGGTTGTTGGTTTGAATGAATTTTATGGAATTCTTGATTGGAATTGGTGTAATGTTAGAAATTCAACTTTCCTTCAAAGGATTTCTGTTCTTCAATCTCCATTTCAAGGGCCAGGATTAGCAGAAAGAAGGGAGTATAATGTGATTTTTCATCCATTAAGTGGGCTTTGTGTGGTGAGAAAATCATTATTAGATCCATTAAGATTGGGCCCATGTGTTGATTCAGATGCTTGGTATTATACTCCACAAAAGTTTCTAACTCTAAAAGGGACTTATTTTTGTATACAAGCAGATGAAATTGGGAAGCAAGCTAAATTGGGGATTATATGTACTGTCTCTAATGCTAAATGGGATATGATTTCTGATTCAAAATTGCATCTTTCTTCAAAGTCTAGTAATGGATCCTTGGTTTGTTTGGACGTAGATTCGAGTACAAATGAAATTGTGACCAATTCTTGTAAGTGTTTGAGTCGGGACTCCTCGTGCGACCCGAGTAGCCAGTGGTTTAAGCTCGTTAATAGTACGAGAAGTTTGGGCAGGGGAAGGTCGATGGTTAACATGGTTGGTTCATCTTTGGCTAATGTTGTGACCAAATTTGTGGAGTTATGAAAAATAATGGAGGAAAAAAAAAAAAAAAGATAATAGAAAAATGCCCCCATGCAGGATCGAACTACAGACCTTCAGTTTACAAGACTGACGCTCTACCACTGAGCTATAGGGGCTGTTGATGTTAACTACGTGTGATTTAGTTACTAATTTGTTTATTAAATTATTAACGTTTACTAACTTAAAAGGACTAATAATTTCAATCTATAAACTCTTGGTATGATTTAAAGGAAAATAATTAACTGCTTCTTCGTATTTATGTGTCATAGGGTATTATTAATTCAAGACTAGTTGCAATTTTAA

Coding sequence (CDS)

ATGAAGGGGTTGATTCTTTTGGTGTGGTGTGTTGCGGCCTCGGCGGCGGTGGGGCTGCCGTTACACACTGATTCACGGTGGATCGTGGACGGAGAAGGGGAAAGAGTGAAACTAAGATGTGTAAATTGGGTGTCGCATTTGGAGGCGGTGGTGGCGGAGGGGCTTTCGAAGCAGCCGATTGAGGAGATTTCGAACAGAATTGAGTGGTTGGGATTCAACTGCGTCAGACTCACTTGGCCACTGTTTTTAGCCACAAATGAGTCTTTGAATTCCCTCACCGTCCGACAGTCTTTCCAGCGCCTCGGCCTGGCCGAAGCCATCGCCGGAATCCAAGCTAACAACCCCTTCATCATCGATCTTCCTCTCTTGAAAGCTTTTGAGGCAGTGGTTGGAAAATTGGGGGAGGGGAAATTAATGGTGATATTGGACAATCACATAAGCAAGCCAGGGTGGTGCTGTAGTAACTTCGACGGAAATGGCTTCTTTGGTGATCAATATTTCAACCCTGACTTATGGATTAAAGGCCTCACTCGAATGGCTACCATGTTTAATGGTGTCAACCATGTTGTTGCTATGAGCCTTAGGAATGAGCTTCGTGGCCCAAAACAAAATGTCAACGACTGGTACAGGTACATGCAAAGAGGAGCAGAAGCAGTTCATTCAGCAAACCCAGACATTCTTATAATCCTCTCAGGACTTAGCTTTGACAGAGACTTATCTTTTCTCAAAAACCAACCCATAAACCTCACATTCACATCCAAAACGGTATATGAAGTTCATTGGTATGCTTTTTCAGATGGATCTTCATGGGAATCAGGAAACTCAAACCAAGTATGTGGAAGAACAACTAACAACTTAATGAAAATGTCTGGATTTCTATTACAACAAGGGTTCCCTCTGTTCATAAGTGAATTTGGAATTGACCAAAGAGGAACAAATGTAAATGACAACAGATATTTGAGCTGTTTTTTAGCTGTAGCTGCTGAATTTGACCTTGACTGGGCACTTTGGACACTTGTTGGAAGCTATTATTTGAGAGAAGGGGTTGTTGGTTTGAATGAATTTTATGGAATTCTTGATTGGAATTGGTGTAATGTTAGAAATTCAACTTTCCTTCAAAGGATTTCTGTTCTTCAATCTCCATTTCAAGGGCCAGGATTAGCAGAAAGAAGGGAGTATAATGTGATTTTTCATCCATTAAGTGGGCTTTGTGTGGTGAGAAAATCATTATTAGATCCATTAAGATTGGGCCCATGTGTTGATTCAGATGCTTGGTATTATACTCCACAAAAGTTTCTAACTCTAAAAGGGACTTATTTTTGTATACAAGCAGATGAAATTGGGAAGCAAGCTAAATTGGGGATTATATGTACTGTCTCTAATGCTAAATGGGATATGATTTCTGATTCAAAATTGCATCTTTCTTCAAAGTCTAGTAATGGATCCTTGGTTTGTTTGGACGTAGATTCGAGTACAAATGAAATTGTGACCAATTCTTGTAAGTGTTTGAGTCGGGACTCCTCGTGCGACCCGAGTAGCCAGTGGTTTAAGCTCGTTAATAGTACGAGAAGTTTGGGCAGGGGAAGGTCGATGGTTAACATGGTTGGTTCATCTTTGGCTAATGTTGTGACCAAATTTGTGGAGTTATGA

Protein sequence

MKGLILLVWCVAASAAVGLPLHTDSRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQPIEEISNRIEWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFIIDLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTRMATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRDLSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGFPLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGILDWNWCNVRNSTFLQRISVLQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLRLGPCVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVSNAKWDMISDSKLHLSSKSSNGSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSMVNMVGSSLANVVTKFVEL
Homology
BLAST of PI0026329 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 4.4e-127
Identity = 247/542 (45.57%), Postives = 320/542 (59.04%), Query Frame = 0

Query: 4   LILLVWCVAASAAVGLPLHTDSRWIVD-GEGERVKLRCVNWVSHLEAVVAEGLSKQPIEE 63
           L  L+  + A+ +  LPL T  RWIVD   G RVKL CVNWV HLE  + EGL++ P+  
Sbjct: 14  LTALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVAT 73

Query: 64  ISNRIEWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFIIDLPL 123
           +++ I  LGFNCVRLT+ + + T  S  + TV Q+F RL L EA +GI+ NNP ++DL  
Sbjct: 74  VAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLGH 133

Query: 124 LKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTRMATM 183
           + A+  VV  L E  +MVILDNH+SKP WCC+  DGNGFFGD+YFNP+ W++GL  MAT 
Sbjct: 134 VAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMATY 193

Query: 184 FNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRDLSFL 243
           FN   +VVAMSLRNELRG +     W R+MQ GA  VH ANP +L+ILSGL FD DLSFL
Sbjct: 194 FNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSFL 253

Query: 244 KNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQ----GF 303
              P+ L F  K VYE HWY+F  G  W +G  N VC   T       GF+         
Sbjct: 254 PVLPVTLPFKEKIVYEGHWYSF--GVPWRTGLPNDVCKNETGRFKSNVGFVTSSANATAA 313

Query: 304 PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLR---EGVVGLNEF 363
           PLF+SEFGIDQR  N NDNRYL+C LA  AE DLDWALWT+ GSYY R   + V    E 
Sbjct: 314 PLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKDFEET 373

Query: 364 YGILDWNWCNVRNSTFLQRISVLQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLR 423
           YG  + +W  +RN  F+ R+  +Q P Q P LA    Y +I+HP SGLC V   + + + 
Sbjct: 374 YGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLC-VESGIGNTVH 433

Query: 424 LGPCVD-SDAWYY--TPQKFLTLKGTYFCIQADEIGKQAKLGIICTV-SNAKWDMISDSK 483
           LG C      W Y  + +  + L G+  CI     G  A +   C+  +N  W  +S ++
Sbjct: 434 LGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTVSSAQ 493

Query: 484 LHLSS----KSSNGSLVCLDVDSSTNEIVTNSCKCLSRDSSC----DPSSQWFKLVNSTR 526
           L L +    K      +CLD  S +  I TN C C++ DS C    +P  QWFK++ + +
Sbjct: 494 LQLGTRVLGKDGKEKWMCLD-GSKSPLISTNECICIT-DSHCYPKLNPEKQWFKVITTNK 550

BLAST of PI0026329 vs. ExPASy TrEMBL
Match: A0A0A0KTH1 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G593400 PE=3 SV=1)

HSP 1 Score: 1115.5 bits (2884), Expect = 0.0e+00
Identity = 533/549 (97.09%), Postives = 543/549 (98.91%), Query Frame = 0

Query: 1   MKGLILLVWCVAASAAVGLPLHTDSRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQPI 60
           MKGLILLVWCVAASAAVGLPLHTD+RWIVDG GERVKLRCVNWVSHLEAVVAEGLSKQPI
Sbjct: 2   MKGLILLVWCVAASAAVGLPLHTDTRWIVDGAGERVKLRCVNWVSHLEAVVAEGLSKQPI 61

Query: 61  EEISNRIEWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFIIDL 120
           EEISNRI+WLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFIIDL
Sbjct: 62  EEISNRIQWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFIIDL 121

Query: 121 PLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTRMA 180
           PLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR+A
Sbjct: 122 PLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTRIA 181

Query: 181 TMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRDLS 240
           TMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLS+DRDLS
Sbjct: 182 TMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSYDRDLS 241

Query: 241 FLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGFPL 300
           FLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGFPL
Sbjct: 242 FLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGFPL 301

Query: 301 FISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGILD 360
           FISEFGIDQRGTNVNDNRYLSCFLAVAAE DLDWA+WTLVGSYYLREGVVGLNEFYGILD
Sbjct: 302 FISEFGIDQRGTNVNDNRYLSCFLAVAAELDLDWAVWTLVGSYYLREGVVGLNEFYGILD 361

Query: 361 WNWCNVRNSTFLQRISVLQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLRLGPCV 420
           WNWCN+RNSTFLQRIS LQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPL LGPCV
Sbjct: 362 WNWCNLRNSTFLQRISALQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLTLGPCV 421

Query: 421 DSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVSNAKWDMISDSKLHLSSKSSN 480
           D+DAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTV+NAKWDMISDSKLHLSSKSSN
Sbjct: 422 DTDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVNNAKWDMISDSKLHLSSKSSN 481

Query: 481 GSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSMVNMVGSSLA 540
           GSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSM+NMVGSSL 
Sbjct: 482 GSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSMINMVGSSLP 541

Query: 541 NVVTKFVEL 550
           NV TKFV+L
Sbjct: 542 NVATKFVDL 550

BLAST of PI0026329 vs. ExPASy TrEMBL
Match: A0A5D3CXK4 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001710 PE=3 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 534/551 (96.91%), Postives = 544/551 (98.73%), Query Frame = 0

Query: 1   MKGLILL--VWCVAASAAVGLPLHTDSRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ 60
           MKGL++L  VWCVAASAAVGLPLHTD+RWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 2   MKGLLILLAVWCVAASAAVGLPLHTDTRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ 61

Query: 61  PIEEISNRIEWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFII 120
           PIEEISNRIE LGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGL EAIAGIQANNPFII
Sbjct: 62  PIEEISNRIEGLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLTEAIAGIQANNPFII 121

Query: 121 DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR 180
           DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR
Sbjct: 122 DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR 181

Query: 181 MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD 240
           MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD
Sbjct: 182 MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD 241

Query: 241 LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF 300
           LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF
Sbjct: 242 LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF 301

Query: 301 PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI 360
           PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI
Sbjct: 302 PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI 361

Query: 361 LDWNWCNVRNSTFLQRISVLQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLRLGP 420
           LDWNWCN+RNSTFLQRISVLQ+P QGPGLAERREYN+IFHPLSGLCVVRKSLLDPLRLGP
Sbjct: 362 LDWNWCNLRNSTFLQRISVLQTPLQGPGLAERREYNLIFHPLSGLCVVRKSLLDPLRLGP 421

Query: 421 CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVSNAKWDMISDSKLHLSSKS 480
           CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTV+NAKWDMISDSKLHLSSKS
Sbjct: 422 CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVNNAKWDMISDSKLHLSSKS 481

Query: 481 SNGSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSMVNMVGSS 540
           SNGSLVCLDVD++TNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLG GRSM+NM GSS
Sbjct: 482 SNGSLVCLDVDANTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGGGRSMINMAGSS 541

Query: 541 LANVVTKFVEL 550
           LANVVTKFVEL
Sbjct: 542 LANVVTKFVEL 552

BLAST of PI0026329 vs. ExPASy TrEMBL
Match: A0A1S3BF06 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103488945 PE=3 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 534/551 (96.91%), Postives = 544/551 (98.73%), Query Frame = 0

Query: 1   MKGLILL--VWCVAASAAVGLPLHTDSRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ 60
           MKGL++L  VWCVAASAAVGLPLHTD+RWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 2   MKGLLILLAVWCVAASAAVGLPLHTDTRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ 61

Query: 61  PIEEISNRIEWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFII 120
           PIEEISNRIE LGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGL EAIAGIQANNPFII
Sbjct: 62  PIEEISNRIEGLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLTEAIAGIQANNPFII 121

Query: 121 DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR 180
           DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR
Sbjct: 122 DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR 181

Query: 181 MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD 240
           MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD
Sbjct: 182 MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD 241

Query: 241 LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF 300
           LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF
Sbjct: 242 LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF 301

Query: 301 PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI 360
           PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI
Sbjct: 302 PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI 361

Query: 361 LDWNWCNVRNSTFLQRISVLQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLRLGP 420
           LDWNWCN+RNSTFLQRISVLQ+P QGPGLAERREYN+IFHPLSGLCVVRKSLLDPLRLGP
Sbjct: 362 LDWNWCNLRNSTFLQRISVLQTPLQGPGLAERREYNLIFHPLSGLCVVRKSLLDPLRLGP 421

Query: 421 CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVSNAKWDMISDSKLHLSSKS 480
           CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTV+NAKWDMISDSKLHLSSKS
Sbjct: 422 CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVNNAKWDMISDSKLHLSSKS 481

Query: 481 SNGSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSMVNMVGSS 540
           SNGSLVCLDVD++TNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLG GRSM+NM GSS
Sbjct: 482 SNGSLVCLDVDANTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGGGRSMINMAGSS 541

Query: 541 LANVVTKFVEL 550
           LANVVTKFVEL
Sbjct: 542 LANVVTKFVEL 552

BLAST of PI0026329 vs. ExPASy TrEMBL
Match: A0A6J1GZ00 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111458804 PE=3 SV=1)

HSP 1 Score: 986.9 bits (2550), Expect = 3.5e-284
Identity = 464/548 (84.67%), Postives = 511/548 (93.25%), Query Frame = 0

Query: 1   MKGLILL--VWCVAASAAVGLPLHTDSRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ 60
           MKGL+LL  V  +   AAVGLPLHTDSRWIVD +G+RVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60

Query: 61  PIEEISNRIEWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFII 120
           PI+EI+NRI  LGFNCVRLTWPLFLATNESL+SLTVRQSFQRLGL EAIAGIQANNPFII
Sbjct: 61  PIDEITNRIGLLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGIQANNPFII 120

Query: 121 DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR 180
           DLPL+KAFEAVVG+LGE +LMV+LDNHISKPGWCCSNFDGNGFFGDQYFNP+ WI+GLTR
Sbjct: 121 DLPLIKAFEAVVGRLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 180

Query: 181 MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD 240
           MATMFNGV HVV MSLRNELRGPKQNVNDWYRYMQRGAEAVH+ANPD+L+ILSGLSFD+D
Sbjct: 181 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 240

Query: 241 LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF 300
           LSFLK QPINLTFT+K VYEVHWYAFSDGSSW+SGN NQVCGR  NNLMKMSGFLL+QG 
Sbjct: 241 LSFLKTQPINLTFTAKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300

Query: 301 PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI 360
           PLF++EFG+DQRGTNVNDNRYLSCFL+VAAE+DLDWA+WTLVGSYYLREGVVGLNEFYG+
Sbjct: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360

Query: 361 LDWNWCNVRNSTFLQRISVLQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLRLGP 420
           LDWNWCN RNS+FLQRIS LQ+PFQGPGLAERR Y+V+FHP +GLCV RKSLLDPLRLGP
Sbjct: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420

Query: 421 CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVSNAKWDMISDSKLHLSSKS 480
           C DSD WYYTPQKFLTLKGTYFCIQA ++GKQ +LGIICTVSNA+WDM+SDSK+HLSSK 
Sbjct: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480

Query: 481 SNGSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSMVNMVGSS 540
            +GS+VCLDVD S+NEIVTN+CKCLSRDSSC+PSSQWFKLVNSTRSLG  RSM++MVGSS
Sbjct: 481 DDGSVVCLDVDPSSNEIVTNACKCLSRDSSCNPSSQWFKLVNSTRSLGAARSMISMVGSS 540

Query: 541 L-ANVVTK 546
           L +N+V K
Sbjct: 541 LSSNLVPK 548

BLAST of PI0026329 vs. ExPASy TrEMBL
Match: A0A6J1KEP8 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111492584 PE=3 SV=1)

HSP 1 Score: 985.7 bits (2547), Expect = 7.7e-284
Identity = 467/552 (84.60%), Postives = 511/552 (92.57%), Query Frame = 0

Query: 1   MKGLILL--VWCVAASAAVGLPLHTDSRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ 60
           MKGL+LL  V  +   AAVGLPLHTDSRWIVD +G+RVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 2   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 61

Query: 61  PIEEISNRIEWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFII 120
           PI+EI+NRI  LGFNCVRLTWPLFLATNESL+SLTVRQSFQRLGL EAIAG+QANNPFII
Sbjct: 62  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 121

Query: 121 DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR 180
           DLPL+KAFEAVVG LGE +LMV+LDNHISKPGWCCSNFDGNGFFGDQYFNP+ WI+GLTR
Sbjct: 122 DLPLIKAFEAVVGWLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 181

Query: 181 MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD 240
           MATMFNGV HVV MSLRNELRGPKQNVNDWYRYMQRGAEAVH+ANPD+L+ILSGLSFD+D
Sbjct: 182 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 241

Query: 241 LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF 300
           LSFLKNQPINLTFT+K VYEVHWYAFSDGSSW+SGN NQVCGR  NNLMKMSGFLL+QG 
Sbjct: 242 LSFLKNQPINLTFTAKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 301

Query: 301 PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI 360
           PLF++EFG+DQRGTNVNDNRYLSCFL+VAAE+DLDWA+WTLVGSYYLREGVVGLNEFYG+
Sbjct: 302 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 361

Query: 361 LDWNWCNVRNSTFLQRISVLQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLRLGP 420
           LDWNWCN RNS+FLQRIS LQ+PFQGPGLAERR Y+V+FHP +GLCV R SLLDPLRLGP
Sbjct: 362 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRASLLDPLRLGP 421

Query: 421 CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVSNAKWDMISDSKLHLSSKS 480
           C DSD WYYTPQKFLTLKGTYFCIQA E+GKQ +LGIICTVSNA+WDM+SDSK+HLSSK 
Sbjct: 422 CADSDPWYYTPQKFLTLKGTYFCIQAQEMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 481

Query: 481 SNGSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSMVNMVGSS 540
            NGS+VCLDVD +TNEIVTN+CKCLSRDSSCDPSSQWFKLVNSTRS    RSM++MVGSS
Sbjct: 482 DNGSVVCLDVDPNTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSFDTTRSMISMVGSS 541

Query: 541 LA-NVVTK-FVE 549
           L+ N+V K FVE
Sbjct: 542 LSFNLVPKLFVE 553

BLAST of PI0026329 vs. NCBI nr
Match: XP_004135502.2 (glycosyl hydrolase 5 family protein [Cucumis sativus] >KGN51727.1 hypothetical protein Csa_008186 [Cucumis sativus])

HSP 1 Score: 1115.5 bits (2884), Expect = 0.0e+00
Identity = 533/549 (97.09%), Postives = 543/549 (98.91%), Query Frame = 0

Query: 1   MKGLILLVWCVAASAAVGLPLHTDSRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQPI 60
           MKGLILLVWCVAASAAVGLPLHTD+RWIVDG GERVKLRCVNWVSHLEAVVAEGLSKQPI
Sbjct: 2   MKGLILLVWCVAASAAVGLPLHTDTRWIVDGAGERVKLRCVNWVSHLEAVVAEGLSKQPI 61

Query: 61  EEISNRIEWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFIIDL 120
           EEISNRI+WLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFIIDL
Sbjct: 62  EEISNRIQWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFIIDL 121

Query: 121 PLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTRMA 180
           PLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR+A
Sbjct: 122 PLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTRIA 181

Query: 181 TMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRDLS 240
           TMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLS+DRDLS
Sbjct: 182 TMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSYDRDLS 241

Query: 241 FLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGFPL 300
           FLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGFPL
Sbjct: 242 FLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGFPL 301

Query: 301 FISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGILD 360
           FISEFGIDQRGTNVNDNRYLSCFLAVAAE DLDWA+WTLVGSYYLREGVVGLNEFYGILD
Sbjct: 302 FISEFGIDQRGTNVNDNRYLSCFLAVAAELDLDWAVWTLVGSYYLREGVVGLNEFYGILD 361

Query: 361 WNWCNVRNSTFLQRISVLQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLRLGPCV 420
           WNWCN+RNSTFLQRIS LQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPL LGPCV
Sbjct: 362 WNWCNLRNSTFLQRISALQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLTLGPCV 421

Query: 421 DSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVSNAKWDMISDSKLHLSSKSSN 480
           D+DAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTV+NAKWDMISDSKLHLSSKSSN
Sbjct: 422 DTDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVNNAKWDMISDSKLHLSSKSSN 481

Query: 481 GSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSMVNMVGSSLA 540
           GSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSM+NMVGSSL 
Sbjct: 482 GSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSMINMVGSSLP 541

Query: 541 NVVTKFVEL 550
           NV TKFV+L
Sbjct: 542 NVATKFVDL 550

BLAST of PI0026329 vs. NCBI nr
Match: XP_008446127.1 (PREDICTED: uncharacterized protein LOC103488945 [Cucumis melo] >KAA0034246.1 Cellulase protein [Cucumis melo var. makuwa] >TYK15674.1 Cellulase protein [Cucumis melo var. makuwa])

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 534/551 (96.91%), Postives = 544/551 (98.73%), Query Frame = 0

Query: 1   MKGLILL--VWCVAASAAVGLPLHTDSRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ 60
           MKGL++L  VWCVAASAAVGLPLHTD+RWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 2   MKGLLILLAVWCVAASAAVGLPLHTDTRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ 61

Query: 61  PIEEISNRIEWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFII 120
           PIEEISNRIE LGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGL EAIAGIQANNPFII
Sbjct: 62  PIEEISNRIEGLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLTEAIAGIQANNPFII 121

Query: 121 DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR 180
           DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR
Sbjct: 122 DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR 181

Query: 181 MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD 240
           MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD
Sbjct: 182 MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD 241

Query: 241 LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF 300
           LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF
Sbjct: 242 LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF 301

Query: 301 PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI 360
           PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI
Sbjct: 302 PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI 361

Query: 361 LDWNWCNVRNSTFLQRISVLQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLRLGP 420
           LDWNWCN+RNSTFLQRISVLQ+P QGPGLAERREYN+IFHPLSGLCVVRKSLLDPLRLGP
Sbjct: 362 LDWNWCNLRNSTFLQRISVLQTPLQGPGLAERREYNLIFHPLSGLCVVRKSLLDPLRLGP 421

Query: 421 CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVSNAKWDMISDSKLHLSSKS 480
           CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTV+NAKWDMISDSKLHLSSKS
Sbjct: 422 CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVNNAKWDMISDSKLHLSSKS 481

Query: 481 SNGSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSMVNMVGSS 540
           SNGSLVCLDVD++TNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLG GRSM+NM GSS
Sbjct: 482 SNGSLVCLDVDANTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGGGRSMINMAGSS 541

Query: 541 LANVVTKFVEL 550
           LANVVTKFVEL
Sbjct: 542 LANVVTKFVEL 552

BLAST of PI0026329 vs. NCBI nr
Match: XP_038893388.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 1053.1 bits (2722), Expect = 8.1e-304
Identity = 502/551 (91.11%), Postives = 527/551 (95.64%), Query Frame = 0

Query: 1   MKGLILL--VWCVAASAAVGLPLHTDSRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ 60
           MKGLIL   V  V   AAVGLPLHTDSRWIVD EG+RVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 8   MKGLILFCSVAAVWLQAAVGLPLHTDSRWIVDEEGQRVKLRCVNWVSHLEAVVAEGLSKQ 67

Query: 61  PIEEISNRIEWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFII 120
           PI+EISNRIE  GFNCVRLTWPLFLATN+SLNSLTVRQSFQRLGLAEAIAGIQANNPFII
Sbjct: 68  PIDEISNRIESFGFNCVRLTWPLFLATNDSLNSLTVRQSFQRLGLAEAIAGIQANNPFII 127

Query: 121 DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR 180
           DLPL+KAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYF+P+LWIKGLTR
Sbjct: 128 DLPLIKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFSPELWIKGLTR 187

Query: 181 MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD 240
           MA MFNGV HVV MSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPD+LIILSGLSFDRD
Sbjct: 188 MAAMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDVLIILSGLSFDRD 247

Query: 241 LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF 300
           LSFLKNQPINLTFT+K VYEVHWY FSDGSSWESGNSNQVCGR TNNLMKMSGF+L+QGF
Sbjct: 248 LSFLKNQPINLTFTAKLVYEVHWYGFSDGSSWESGNSNQVCGRVTNNLMKMSGFILEQGF 307

Query: 301 PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI 360
           PLFISEFG+DQRGTN+NDNRYLSCFL+VAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI
Sbjct: 308 PLFISEFGVDQRGTNINDNRYLSCFLSVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI 367

Query: 361 LDWNWCNVRNSTFLQRISVLQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLRLGP 420
           LDWNWC++RNS+FLQRIS LQSPFQGPGL ERR+YNVIFHPL+GLCVVRKSLLDPL+LGP
Sbjct: 368 LDWNWCSLRNSSFLQRISALQSPFQGPGLEERRQYNVIFHPLTGLCVVRKSLLDPLKLGP 427

Query: 421 CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVSNAKWDMISDSKLHLSSKS 480
           CVDSD WYYTPQKFLTLKGTYFCIQA+E+GKQAKLGIICTVSNAKWDMISDSK+HLSSK 
Sbjct: 428 CVDSDPWYYTPQKFLTLKGTYFCIQAEEMGKQAKLGIICTVSNAKWDMISDSKMHLSSKV 487

Query: 481 SNGSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSMVNMVGSS 540
           SNGS+VCLDVDSSTNEIVTNSCKCL RDS+CDPSSQWFKLVNSTRSLGR RSMV+MVGSS
Sbjct: 488 SNGSMVCLDVDSSTNEIVTNSCKCLGRDSACDPSSQWFKLVNSTRSLGRARSMVSMVGSS 547

Query: 541 LANVVTKFVEL 550
           L NVVTKFVEL
Sbjct: 548 LGNVVTKFVEL 558

BLAST of PI0026329 vs. NCBI nr
Match: XP_023514558.1 (uncharacterized protein LOC111778811 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 989.9 bits (2558), Expect = 8.5e-285
Identity = 466/548 (85.04%), Postives = 511/548 (93.25%), Query Frame = 0

Query: 1   MKGLILL--VWCVAASAAVGLPLHTDSRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ 60
           MKGL+LL  V  +   AAVGLPLHTDSRWIVD +G+RVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60

Query: 61  PIEEISNRIEWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFII 120
           PI+EI+NRI  LGFNCVRLTWPLFLATNESL+SLTVRQSFQRLGL EAIAG+QANNPFII
Sbjct: 61  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 120

Query: 121 DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR 180
           DLPL+KAFEAVVG+LGE +LMV+LDNHISKPGWCCSNFDGNGFFGDQYFNP+ WI+GLTR
Sbjct: 121 DLPLIKAFEAVVGRLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 180

Query: 181 MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD 240
           MATMFNGV HVV MSLRNELRGPKQNVNDWYRYMQRGAEAVH+ANPD+L+ILSGLSFD+D
Sbjct: 181 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 240

Query: 241 LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF 300
           LSFLK QPINLTFTSK VYEVHWYAFSDGSSW+SGN NQVCGR  NNLMKMSGFLL+QG 
Sbjct: 241 LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300

Query: 301 PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI 360
           PLF++EFG+DQRGTNVNDNRYLSCFL+VAAE+DLDWA+WTLVGSYYLREGVVGLNEFYG+
Sbjct: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360

Query: 361 LDWNWCNVRNSTFLQRISVLQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLRLGP 420
           LDWNWCN RNS+FLQRIS LQ+PFQGPGLAERR Y+V+FHP +GLCV RKSLLDPLRLGP
Sbjct: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420

Query: 421 CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVSNAKWDMISDSKLHLSSKS 480
           C DSD WYYTPQKFLTLKGTYFCIQA ++GKQ +LGIICTVSNA+WDM+SDSK+HLSSK 
Sbjct: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480

Query: 481 SNGSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSMVNMVGSS 540
            +GS+VCLDVD STNEIVTN+CKCLSRDSSCDPSSQWFKLVNSTRSLG  RSM++MVGSS
Sbjct: 481 DDGSVVCLDVDPSTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGATRSMISMVGSS 540

Query: 541 L-ANVVTK 546
           L +N+V K
Sbjct: 541 LSSNLVPK 548

BLAST of PI0026329 vs. NCBI nr
Match: KAG7032321.1 (hypothetical protein SDJN02_06366, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 988.0 bits (2553), Expect = 3.2e-284
Identity = 464/548 (84.67%), Postives = 512/548 (93.43%), Query Frame = 0

Query: 1   MKGLILL--VWCVAASAAVGLPLHTDSRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQ 60
           M+GL+LL  V  +   AAVGLPLHTDSRWIVD +G+RVKLRCVNWVSHLEAVVAEGLSKQ
Sbjct: 1   MRGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60

Query: 61  PIEEISNRIEWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFII 120
           PI+EI+NRI  LGFNCVRLTWPLFLATNESL+SLTVRQSFQRLGL EAIAGIQANNPFII
Sbjct: 61  PIDEITNRIGLLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGIQANNPFII 120

Query: 121 DLPLLKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTR 180
           DLPL+KAFEAVVG+LGE +LMV+LDNHISKPGWCCSNFDGNGFFGDQYFNP+ WI+GLTR
Sbjct: 121 DLPLIKAFEAVVGRLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 180

Query: 181 MATMFNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRD 240
           MATMFNGV HVV MSLRNELRGPKQNVNDWYRYMQRGAEAVH+ANPD+L+ILSGLSFD+D
Sbjct: 181 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 240

Query: 241 LSFLKNQPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGF 300
           LSFLK QPINLTFTSK VYEVHWYAFSDGSSW+SGN NQVCGR  NNL+KMSGFLL+QG 
Sbjct: 241 LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLLKMSGFLLEQGM 300

Query: 301 PLFISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI 360
           PLF++EFG+DQRGTNVNDNRYLSCFL+VAAE+DLDWA+WTLVGSYYLREGVVGLNEFYG+
Sbjct: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360

Query: 361 LDWNWCNVRNSTFLQRISVLQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLRLGP 420
           LDWNWCN RNS+FLQRIS LQ+PFQGPGLAERR Y+V+FHP +GLCV RKSLLDPLRLGP
Sbjct: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420

Query: 421 CVDSDAWYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVSNAKWDMISDSKLHLSSKS 480
           C DSD WYYTPQKFLTLKGTYFCIQA ++GKQ +LGIICTVSNA+WDM+SDS++HLSSK 
Sbjct: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSRMHLSSKL 480

Query: 481 SNGSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSMVNMVGSS 540
            +GS+VCLDVD STNEIVTN+CKCLS+DSSCDPSSQWFKLVNSTRSLGR RSM++MVGSS
Sbjct: 481 EDGSVVCLDVDPSTNEIVTNACKCLSQDSSCDPSSQWFKLVNSTRSLGRTRSMISMVGSS 540

Query: 541 L-ANVVTK 546
           L +N+V K
Sbjct: 541 LSSNLVPK 548

BLAST of PI0026329 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 659.8 bits (1701), Expect = 1.9e-189
Identity = 307/514 (59.73%), Postives = 392/514 (76.26%), Query Frame = 0

Query: 20  PLHTDSRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQPIEEISNRIEWLGFNCVRLTW 79
           PL T SRWIVD  G RVKL C NW SHL+ VVAEGLSKQP++ ++ +I  +GFNCVRLTW
Sbjct: 34  PLSTSSRWIVDENGLRVKLVCANWPSHLQPVVAEGLSKQPVDAVAKKIVEMGFNCVRLTW 93

Query: 80  PLFLATNESL-NSLTVRQSFQRLGLAEAIAGIQANNPFIIDLPLLKAFEAVVGKLGEGKL 139
           PL L TNE+L N++TVRQSFQ LGL + I G Q NNP IIDLPL++A++ VV  LG   +
Sbjct: 94  PLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSIIDLPLIEAYKTVVTTLGNNDV 153

Query: 140 MVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTRMATMFNGVNHVVAMSLRNEL 199
           MVILDNH++KPGWCC+N DGNGFFGDQ+F+P +W+  L +MA  FNGV++VV MSLRNEL
Sbjct: 154 MVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALKKMAATFNGVSNVVGMSLRNEL 213

Query: 200 RGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRDLSFLKNQPINLTFTSKTVYE 259
           RGPKQNVNDW++YMQ+GAEAVHSAN  +L+ILSGLSFD DLSF++++P+ L+FT K V+E
Sbjct: 214 RGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSFDADLSFVRSRPVKLSFTGKLVFE 273

Query: 260 VHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGFPLFISEFGIDQRGTNVNDNR 319
           +HWY+FSDG+SW + N N +CGR  N +    G+LL QGFPLF+SEFGID+RG N NDNR
Sbjct: 274 LHWYSFSDGNSWAANNPNDICGRVLNRIGNGGGYLLNQGFPLFLSEFGIDERGVNTNDNR 333

Query: 320 YLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGILDWNWCNVRNSTFLQRISVL 379
           Y  C    AAE D+DW+LW L GSYYLR+G VG+NE+YG+LD +W +VRNS+FLQ+IS L
Sbjct: 334 YFGCLTGWAAENDVDWSLWALTGSYYLRQGKVGMNEYYGVLDSDWISVRNSSFLQKISFL 393

Query: 380 QSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDP--LRLGPCVDSDAWYYTPQKFLTLK 439
           QSP QGPG      YN++FHPL+GLC+VR SL DP  L LGPC  S+ W YT +K L +K
Sbjct: 394 QSPLQGPG-PRTDAYNLVFHPLTGLCIVR-SLDDPKMLTLGPCNSSEPWSYT-KKALRIK 453

Query: 440 GTYFCIQADEIGKQAKL-GIICTVSNAKWDMISDSKLHLSSKSSNGSLVCLDVDSSTNEI 499
               C+Q++       +    C+ S +KW  IS S++HL+S +SN + +CLDVD++ N +
Sbjct: 454 DQQLCLQSNGPKNPVTMTRTSCSTSGSKWQTISASRMHLASTTSNKTSLCLDVDTA-NNV 513

Query: 500 VTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGR 530
           V N+CKCLS+D SC+P SQWFK++ +TR L   R
Sbjct: 514 VANACKCLSKDKSCEPMSQWFKIIKATRPLKSSR 543

BLAST of PI0026329 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 632.9 bits (1631), Expect = 2.4e-181
Identity = 300/521 (57.58%), Postives = 395/521 (75.82%), Query Frame = 0

Query: 20  PLHTDSRWIVD--GEGERVKLRCVNWVSHLEAVVAEGLSKQPIEEISNRIEWLGFNCVRL 79
           P  TDSRWIVD   +G RVKL CVNW SHLE  VAEGLSKQP++ I+ +I  +GFNCVRL
Sbjct: 22  PPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQPLDAIAEKIVSMGFNCVRL 81

Query: 80  TWPLFLATNESLNS-LTVRQSFQRLGLAEAIAGIQANNPFIIDLPLLKAFEAVVGKLGEG 139
           TWPL+LAT+ES ++ +TVRQS ++  L EA++G Q +NP I+DLPL+KAF+ VV  L + 
Sbjct: 82  TWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTILDLPLIKAFQEVVYCLEKH 141

Query: 140 KLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTRMATMFNGV-NHVVAMSLR 199
           ++MVILDNHIS+PGWCCS+ DGNGFFGD++ NP +WIKGL +MA+MF  V ++VV MSLR
Sbjct: 142 RVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLKKMASMFANVSSNVVGMSLR 201

Query: 200 NELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRDLSFLKNQPINLTFTSKT 259
           NELRGPKQN+ DWY+YM+ GAEAVHS NP++L+I+SGL++  DLSFL+ +P  ++F  K 
Sbjct: 202 NELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNYATDLSFLRERPFEVSFRRKV 261

Query: 260 VYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGFPLFISEFGIDQRGTNVN 319
           V+E+HWY F   ++WE  N N++CG+ T  +MKMSGFLL++G PLF+SEFGIDQRG N N
Sbjct: 262 VFEIHWYGF--WNTWEGDNLNKICGKETEKMMKMSGFLLEKGIPLFVSEFGIDQRGNNAN 321

Query: 320 DNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGILDWNWCNVRNSTFLQRI 379
           DN++LSCF+A+AA+ DLDW+LWTL GSYY+RE  +G +E YG+LD+NW ++RNST LQ I
Sbjct: 322 DNKFLSCFMALAADRDLDWSLWTLAGSYYIREKSIGSDESYGVLDFNWSSIRNSTILQMI 381

Query: 380 SVLQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLRLGPCVDSDAWYYTPQKFLTL 439
           S +Q+PF   GL E +   ++FHP +GLC+VRKSL   L+LG C  S++W  +  + L+L
Sbjct: 382 SAIQTPF--IGLMETQPKKIMFHPSTGLCIVRKSLFQ-LKLGSCNRSESWRLSSHRVLSL 441

Query: 440 -KGTYFCIQADEIGKQAKLGIICTVSN-AKWDMISDSKLHLSSKSSNGSLVCLDVDSSTN 499
            +    C++A E GK  KL +  + S  +KW + SDSK+ LSS + NG  VCLDVD+  N
Sbjct: 442 AEEQILCLKAYEKGKSVKLRLFFSESYCSKWKLFSDSKMQLSSITKNGFSVCLDVDTENN 501

Query: 500 EIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSMVNM 535
            IVTNSCKCL  +SSCDP SQWFKLV STR   R +  + +
Sbjct: 502 NIVTNSCKCLRGNSSCDPRSQWFKLVTSTRRRSRPKPFLQI 537

BLAST of PI0026329 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 611.7 bits (1576), Expect = 5.8e-175
Identity = 289/510 (56.67%), Postives = 389/510 (76.27%), Query Frame = 0

Query: 20  PLHTDSRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQPIEEISNRIEWLGFNCVRLTW 79
           PL T+SRWI+D +G+RVKL CVNW SHL+ VVAEGLSKQ +++++ +I  +GFNCVR TW
Sbjct: 4   PLSTNSRWIIDEKGQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFTW 63

Query: 80  PLFLATNESL-NSLTVRQSFQRLGLAEAIAGIQANNPFIIDLPLLKAFEAVVGKLGEGKL 139
           PL LATNE+L N++TVRQSFQ LGL + I+G +  NP +IDLPL++A++ VV KLG   +
Sbjct: 64  PLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNNV 123

Query: 140 MVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTRMATMFNGVNHVVAMSLRNEL 199
           MVILDNH++KPGWCC   DGNGFFGD +F+P  WI GLT++A  F G  +VV MSLRNEL
Sbjct: 124 MVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNEL 183

Query: 200 RGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRDLSFLKNQPINLTFTSKTVYE 259
           RGPKQNV+DW++YMQ+GAEAVH ANP++L+ILSGLS+D DLSF++++ +NLTFT K V+E
Sbjct: 184 RGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNLTFTRKLVFE 243

Query: 260 VHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGFPLFISEFGIDQRGTNVNDNR 319
           +H Y+F++ ++W S N N+ CG    ++    GF L+  FP+F+SEFGID RG NVNDNR
Sbjct: 244 LHRYSFTNTNTWSSKNPNEACGEILKSIENGGGFNLRD-FPVFLSEFGIDLRGKNVNDNR 303

Query: 320 YLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGILDWNWCNVRNSTFLQRISVL 379
           Y+ C L  AAE D+DW++WTL GSYYLREGVVG++EFYGILD +W  VR+ +FLQR+S++
Sbjct: 304 YIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFLQRLSLI 363

Query: 380 QSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLR--LGPCVDSDAWYYTPQKFLTLK 439
            SP QGPG ++ + YN++FHPL+GLC++ +S+LDP +  LG C +S  W YTPQ  LTLK
Sbjct: 364 LSPLQGPG-SQSKVYNLVFHPLTGLCML-QSILDPTKVTLGLCNESQPWSYTPQNTLTLK 423

Query: 440 GTYFCIQADEIGKQAKLG-IICTVSN-AKWDMISDSKLHLSSKSSNGSLVCLDVDSSTNE 499
               C+++       KL    C+  N ++W+ IS S + L++KS+N SL CLDVD  TN 
Sbjct: 424 DKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLAAKSTNNSL-CLDVD-ETNN 483

Query: 500 IVTNSCKCL-SRDSSCDPSSQWFKLVNSTR 524
           ++ ++CKC+   DSSCDP SQWFK+V  ++
Sbjct: 484 LMASNCKCVKGEDSSCDPISQWFKIVKVSK 508

BLAST of PI0026329 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 580.5 bits (1495), Expect = 1.4e-165
Identity = 280/521 (53.74%), Postives = 371/521 (71.21%), Query Frame = 0

Query: 4   LILLVWCVAASAAVGLPLHTDSRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQPIEEI 63
           L L +  ++ + A   PL T SRWIV+ +G RVKL C NW SHL+ VVAEGLS QP++ I
Sbjct: 11  LFLFLSLISLTLATDYPLFTKSRWIVNNKGHRVKLACANWPSHLKPVVAEGLSSQPMDSI 70

Query: 64  SNRIEWLGFNCVRLTWPLFLATNESLN-SLTVRQSFQRLGLAEAIAGIQANNPFIIDLPL 123
           S +I+ +GFNCVRLTWPL L  N++L  ++TV+QSF+R GL   + GI  +NP+I++ PL
Sbjct: 71  SKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTHNPYIVNTPL 130

Query: 124 LKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTRMATM 183
           +  F+AVV  LG   +MVILDNH + PGWCCSN D + FFGD  FNPDLW+ GL +MAT+
Sbjct: 131 INVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWMLGLKKMATI 190

Query: 184 FNGVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRDLSFL 243
           F  V +VV MSLRNELRG      DWY+YMQ+GAEAVH++NP++L+ILSGL+FD DLSFL
Sbjct: 191 FMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSGLNFDADLSFL 250

Query: 244 KNQPINLTFTSKTVYEVHWYAFSDGS-SWESGNSNQVCGRTTNNLMKMSGFLLQQGFPLF 303
           K++P+NL+F  K V E+HWY+F+DG+  W+S N N  C +  +   +  GF+L QGFPLF
Sbjct: 251 KDRPVNLSFKKKLVLELHWYSFTDGTGQWKSHNVNDFCSQMFSKERRTGGFVLDQGFPLF 310

Query: 304 ISEFGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGILDW 363
           +SEFG DQRG ++  NRY++C LA AAE DLDWA+W + G YY REG  G+ E YG+LD 
Sbjct: 311 LSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREGKRGVVEAYGMLDA 370

Query: 364 NWCNVRNSTFLQRISVLQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLL--DPLRLGPC 423
           NW NV N T+L+R+SV+Q P  GPG+ +   +  IFHPL+GLC+VRKS      L LGPC
Sbjct: 371 NWHNVHNYTYLRRLSVIQPPHTGPGV-KHNHHKKIFHPLTGLCLVRKSHCHESELTLGPC 430

Query: 424 VDSDAWYYTPQKFLTL-KGTYFCIQAD-EIGKQAKLGIICTVSNAKWDMISDSKLHLSSK 483
              + W Y+    L + +G   C++ +  +GK  KLG ICT    K + IS +K+HLS  
Sbjct: 431 TKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRICT----KIEQISATKMHLSFN 490

Query: 484 SSNGSLVCLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKL 519
           +S+GSLVCLDVDS  N +V NSC CL+ D++C+P+SQWFK+
Sbjct: 491 TSDGSLVCLDVDSD-NNVVANSCNCLTGDTTCEPASQWFKI 525

BLAST of PI0026329 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 497.3 bits (1279), Expect = 1.6e-140
Identity = 248/505 (49.11%), Postives = 331/505 (65.54%), Query Frame = 0

Query: 20  PLHTDSRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQPIEEISNRIEWLGFNCVRLTW 79
           PL T SRWIVD +G+RVKL CVNW +HL+  VAEGLSKQP++ IS +I  +GFNCVRLTW
Sbjct: 25  PLSTKSRWIVDEKGQRVKLACVNWPAHLQPTVAEGLSKQPLDSISKKIVSMGFNCVRLTW 84

Query: 80  PLFLATNESLN-SLTVRQSFQRLGLAEAIAGIQANNPFIIDLPLLKAFEAVVGKLGEGKL 139
           PL L TN++L   +TV+QSF+ L L E + GIQ +NP ++ LPL  AF+ VV  LGE  +
Sbjct: 85  PLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKLLHLPLFNAFQEVVSNLGENGV 144

Query: 140 MVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTRMATMFNGVNHVVAMSLRNEL 199
           MVILDNH++ PGWCC + D + FFG  +F+P +W KGL +MAT+F    HV+ MSLRNE 
Sbjct: 145 MVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLRKMATLFRNFTHVIGMSLRNEP 204

Query: 200 RGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRDLSFLKNQPINLTFTSKTVYE 259
           RG +   + W+R+M +GAEAVH+ANP +L+ILSG+ FD +LSFL+++ +N++FT K V+E
Sbjct: 205 RGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFDTNLSFLRDRSVNVSFTDKLVFE 264

Query: 260 VHWYAFSDG-SSWESGNSNQVCGRTTNNLMKMSGFLLQQGFPLFISEFGIDQRGTNVNDN 319
           +HWY+FSDG  SW   NSN  C +    +    GFLL +GFPL +SEFG DQRG +++ N
Sbjct: 265 LHWYSFSDGRDSWRKHNSNDFCVKIIEKVTHNGGFLLGRGFPLILSEFGTDQRGGDMSGN 324

Query: 320 RYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGILDWNWCNVRNSTFLQRISV 379
           RY++C +A AAE DLDWA+W L G YYLR                               
Sbjct: 325 RYMNCLVAWAAENDLDWAVWALTGDYYLR------------------------------- 384

Query: 380 LQSPFQGPGLAERREYNVIFHPLSGLCVVR--KSLLDPLRLGPCVDSDAWYYTPQKFLTL 439
                 GPGL  R   N++FHP +GLCV       +  LRLGPC  SD W + P + + L
Sbjct: 385 -----TGPGL--RPNKNLLFHPSTGLCVTNNPSDNIPTLRLGPCPKSDPWTFNPSEGI-L 444

Query: 440 KGTYFCIQA-DEIGKQAKLGIICTVSNAKWDMISDSKLHLSSKSSNGSLVCLDVDSSTNE 499
                C++A + +G++ KLG+    S  K   IS +K+HLS K+SNG L+CLDVD   N 
Sbjct: 445 WINKMCVEAPNVVGQKVKLGVGTKCS--KLGQISATKMHLSFKTSNGLLLCLDVDERDNS 488

Query: 500 IVTNSCKCLSRDSSCDPSSQWFKLV 520
           +V N CK L+ D+SCDP+SQWFK++
Sbjct: 505 VVANRCKFLTMDASCDPASQWFKVL 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0HLA04.4e-12745.57Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KTH10.0e+0097.09Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G593400 PE=3... [more]
A0A5D3CXK40.0e+0096.91Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BF060.0e+0096.91Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103488945 PE=3 SV... [more]
A0A6J1GZ003.5e-28484.67Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111458804 P... [more]
A0A6J1KEP87.7e-28484.60Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111492584 PE=... [more]
Match NameE-valueIdentityDescription
XP_004135502.20.0e+0097.09glycosyl hydrolase 5 family protein [Cucumis sativus] >KGN51727.1 hypothetical p... [more]
XP_008446127.10.0e+0096.91PREDICTED: uncharacterized protein LOC103488945 [Cucumis melo] >KAA0034246.1 Cel... [more]
XP_038893388.18.1e-30491.11glycosyl hydrolase 5 family protein-like [Benincasa hispida][more]
XP_023514558.18.5e-28585.04uncharacterized protein LOC111778811 [Cucurbita pepo subsp. pepo][more]
KAG7032321.13.2e-28484.67hypothetical protein SDJN02_06366, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
AT1G13130.11.9e-18959.73Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26130.12.4e-18157.58Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26140.15.8e-17556.67Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.11.4e-16553.74Glycosyl hydrolase superfamily protein [more]
AT5G16700.11.6e-14049.11Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 19..379
e-value: 6.8E-74
score: 251.2
NoneNo IPR availablePANTHERPTHR31263:SF44CELLULASE (GLYCOSYL HYDROLASE FAMILY 5) PROTEINcoord: 5..527
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 5..527
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 59..339
e-value: 6.8E-26
score: 91.3
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 392..518
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 20..371

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0026329.1PI0026329.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds