PI0025973 (gene) Melon (PI 482460) v1

Overview
NamePI0025973
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionRegulator of Vps4 activity in the MVB pathway protein
Locationchr03: 16734362 .. 16736185 (-)
RNA-Seq ExpressionPI0025973
SyntenyPI0025973
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACGCGCTTCTCCTTCACTCTTCCTTGTTTTCCCCCTGCCTCTCCACTCTGTAACAACTTTCATATATAAACAAAATCTCATTCAAAATTCCATCCAATCCCCAATTAACCCTTTTCAAATTCCATTCCATTTGGCCATGGGAAGAAAGCTTGACGCTCTTCTTGGTAGGAATTTCAGAGCCTCCAAATTTCGCCCTCTTCTCAATCTCTCCCTCTCTCGTCTTTCCATTCTCACCGCCCAACGCCGTGTCCAATTCTCTCAGGCCAATTCCGACGTCCTTCAACTCCTTCAACTCTCCCACCACCACCGCGCTTTACTTCGAGTACGTTTCTCTTCTTCCCCCTTTTTTTCTCTCTTCTTTTCATTTCCCCATTTCATTTTATTCATTTCTTTTCTGTTTTTTCATTCCTAGGTCGAAAAAGTCATTAAGGATCGGAATGCTCTGGATGCTTATGTTTTAATTGAAGGCTACCTTAATCTCTTGGTTGAAAGAACCACCCTCCTCGAACAACAAAGGTCTATTTTCTTATTGATCTCTCCTCTAATCTCCTTCTTAATCATTTTATTTCATTTTACTTACTGTTTTTTTTTTTTTTTAAATTTCAGTGAGTGCCCTGAGGAATTGAAAGAGGCAGTTGCTGGTTTACTCTTCGCAGCTTCAAGATGTGGGGATTTCCCTGAACTTCATGAGATCAAATCAGTTTTGACCACTCGTTTTGGCAAGGAATTCACTGCTCGTGCTGTTGAATTACGCAACAATTGTGGAGTTAATCTTTTGGTAAATTTTGGGTTCTCATTAGTCACTCCTGACTCATTCCTCTGTTTCCTTCGTTTATTCATTGTTTGCTTTGTCTCTGTAGTTAATGCAAAAACTTTCCACAAGGCAGCCCACTTTGGAGACTAGAATGGAGGTCCTCAAATCCATTGCTTCCGAGAATGGGATTCTATTGCAAATTGACCAACTTCCTCCTTCCAATGAGGTATACAACTTGAACTTGACATTTAATTTTACTGCTTACAGCTGTGTTTTGGTAGCAGAGTCAAAACCCGAAATCTTGAATCTTCTTAAAACCCAATTTGGGATTCTTTTTCTGGTTATCGCAGGAAAAAGTAGCCAGAAATGGGAGGCAAAGCGAGGCAGAGGGACAGAGTGAAGAATTTTCAAATGAAGTGGCATCTGGTTCTAAAAGAACGTACAAGGATGTGGCGGATGCAGCACAAGCCGCTTTTGAATCAGCAGCTCAAGCAGCAGCTGCTGCACGAGCTGCTATGGAACTCTCCCGATCCCATGAGGGTCCAGGTAGCCCGAGTAAGCCTGGCTCTGGAACAACTCCACATAACAAACAAAAGGAAGAGAAAACAGAGGTTGAATCTCAAGCAAAACAAGAAATGGAGGAATATAGAAATAGGAAGAAAGGAGAAGGAGAAGGAGAAGGAGAAGAAGAAGAAGGGGGGAAAATGGATGAGGAGAGAACTTCAAATACTGAAAATGGGTTGAAGATGGAAACAAAGGTAGAGAAAACAGAGGTAAGTGAGAAGGGAAGTTTTCGTTTAAATCTAGAGAAGAAACCAATTTCAGTAAGAACAAGAAGAGTACGAGGATACTGAACAAGAACATATATATGTAGTTCATAATTGGGTTTGCTTTTGGATTCTTTCATCATATATATAAATAAATATATATATTTTTGGTTGCGGGAATATCATGGAAACTGTGTAATCATGTAGTTTGGGAATTCTGGTATTGTATGGTAAGTACAACATTTTTTTTTTGTTATACAATGGTATGTATATTCACATTAATATTTGACAACCAATTTTTT

mRNA sequence

CACGCGCTTCTCCTTCACTCTTCCTTGTTTTCCCCCTGCCTCTCCACTCTGTAACAACTTTCATATATAAACAAAATCTCATTCAAAATTCCATCCAATCCCCAATTAACCCTTTTCAAATTCCATTCCATTTGGCCATGGGAAGAAAGCTTGACGCTCTTCTTGGTAGGAATTTCAGAGCCTCCAAATTTCGCCCTCTTCTCAATCTCTCCCTCTCTCGTCTTTCCATTCTCACCGCCCAACGCCGTGTCCAATTCTCTCAGGCCAATTCCGACGTCCTTCAACTCCTTCAACTCTCCCACCACCACCGCGCTTTACTTCGAGTCGAAAAAGTCATTAAGGATCGGAATGCTCTGGATGCTTATGTTTTAATTGAAGGCTACCTTAATCTCTTGGTTGAAAGAACCACCCTCCTCGAACAACAAAGTGAGTGCCCTGAGGAATTGAAAGAGGCAGTTGCTGGTTTACTCTTCGCAGCTTCAAGATGTGGGGATTTCCCTGAACTTCATGAGATCAAATCAGTTTTGACCACTCGTTTTGGCAAGGAATTCACTGCTCGTGCTGTTGAATTACGCAACAATTGTGGAGTTAATCTTTTGTTAATGCAAAAACTTTCCACAAGGCAGCCCACTTTGGAGACTAGAATGGAGGTCCTCAAATCCATTGCTTCCGAGAATGGGATTCTATTGCAAATTGACCAACTTCCTCCTTCCAATGAGGAAAAAGTAGCCAGAAATGGGAGGCAAAGCGAGGCAGAGGGACAGAGTGAAGAATTTTCAAATGAAGTGGCATCTGGTTCTAAAAGAACGTACAAGGATGTGGCGGATGCAGCACAAGCCGCTTTTGAATCAGCAGCTCAAGCAGCAGCTGCTGCACGAGCTGCTATGGAACTCTCCCGATCCCATGAGGGTCCAGGTAGCCCGAGTAAGCCTGGCTCTGGAACAACTCCACATAACAAACAAAAGGAAGAGAAAACAGAGGTTGAATCTCAAGCAAAACAAGAAATGGAGGAATATAGAAATAGGAAGAAAGGAGAAGGAGAAGGAGAAGGAGAAGAAGAAGAAGGGGGGAAAATGGATGAGGAGAGAACTTCAAATACTGAAAATGGGTTGAAGATGGAAACAAAGGTAGAGAAAACAGAGGTAAGTGAGAAGGGAAGTTTTCGTTTAAATCTAGAGAAGAAACCAATTTCAGTAAGAACAAGAAGAGTACGAGGATACTGAACAAGAACATATATATGTAGTTCATAATTGGGTTTGCTTTTGGATTCTTTCATCATATATATAAATAAATATATATATTTTTGGTTGCGGGAATATCATGGAAACTGTGTAATCATGTAGTTTGGGAATTCTGGTATTGTATGGTAAGTACAACATTTTTTTTTTGTTATACAATGGTATGTATATTCACATTAATATTTGACAACCAATTTTTT

Coding sequence (CDS)

ATGGGAAGAAAGCTTGACGCTCTTCTTGGTAGGAATTTCAGAGCCTCCAAATTTCGCCCTCTTCTCAATCTCTCCCTCTCTCGTCTTTCCATTCTCACCGCCCAACGCCGTGTCCAATTCTCTCAGGCCAATTCCGACGTCCTTCAACTCCTTCAACTCTCCCACCACCACCGCGCTTTACTTCGAGTCGAAAAAGTCATTAAGGATCGGAATGCTCTGGATGCTTATGTTTTAATTGAAGGCTACCTTAATCTCTTGGTTGAAAGAACCACCCTCCTCGAACAACAAAGTGAGTGCCCTGAGGAATTGAAAGAGGCAGTTGCTGGTTTACTCTTCGCAGCTTCAAGATGTGGGGATTTCCCTGAACTTCATGAGATCAAATCAGTTTTGACCACTCGTTTTGGCAAGGAATTCACTGCTCGTGCTGTTGAATTACGCAACAATTGTGGAGTTAATCTTTTGTTAATGCAAAAACTTTCCACAAGGCAGCCCACTTTGGAGACTAGAATGGAGGTCCTCAAATCCATTGCTTCCGAGAATGGGATTCTATTGCAAATTGACCAACTTCCTCCTTCCAATGAGGAAAAAGTAGCCAGAAATGGGAGGCAAAGCGAGGCAGAGGGACAGAGTGAAGAATTTTCAAATGAAGTGGCATCTGGTTCTAAAAGAACGTACAAGGATGTGGCGGATGCAGCACAAGCCGCTTTTGAATCAGCAGCTCAAGCAGCAGCTGCTGCACGAGCTGCTATGGAACTCTCCCGATCCCATGAGGGTCCAGGTAGCCCGAGTAAGCCTGGCTCTGGAACAACTCCACATAACAAACAAAAGGAAGAGAAAACAGAGGTTGAATCTCAAGCAAAACAAGAAATGGAGGAATATAGAAATAGGAAGAAAGGAGAAGGAGAAGGAGAAGGAGAAGAAGAAGAAGGGGGGAAAATGGATGAGGAGAGAACTTCAAATACTGAAAATGGGTTGAAGATGGAAACAAAGGTAGAGAAAACAGAGGTAAGTGAGAAGGGAAGTTTTCGTTTAAATCTAGAGAAGAAACCAATTTCAGTAAGAACAAGAAGAGTACGAGGATACTGA

Protein sequence

MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRALLRVEKVIKDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELHEIKSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASENGILLQIDQLPPSNEEKVARNGRQSEAEGQSEEFSNEVASGSKRTYKDVADAAQAAFESAAQAAAAARAAMELSRSHEGPGSPSKPGSGTTPHNKQKEEKTEVESQAKQEMEEYRNRKKGEGEGEGEEEEGGKMDEERTSNTENGLKMETKVEKTEVSEKGSFRLNLEKKPISVRTRRVRGY
Homology
BLAST of PI0025973 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.0e-10
Identity = 50/174 (28.74%), Postives = 88/174 (50.57%), Query Frame = 0

Query: 10  GRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRALLRVEKVIKD 69
           G ++ + K +  L L++SR+ IL  ++         +V +LL+  +   A +RVE +I+D
Sbjct: 5   GPSYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRD 64

Query: 70  RNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELHEIKSV 129
              ++ + +IE    LL  R  L+   +E P E+KE++  L++++ R    PEL +IK+ 
Sbjct: 65  EYLIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQ 124

Query: 130 LTTRFGKEFTARAVELRNNC----GVNLLLMQKLSTRQPTLETRMEVLKSIASE 180
           L  ++GK      +E   NC     VN  ++ KLS   P      + L  IA +
Sbjct: 125 LKAKYGK-----GLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEK 172

BLAST of PI0025973 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.1e-09
Identity = 46/158 (29.11%), Postives = 79/158 (50.00%), Query Frame = 0

Query: 8   LLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRALLRVEKVI 67
           +LG  F+A + R  L L ++RL +L  ++     +A  ++   L      RA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRC-GDFPELHEI 127
           ++   ++A  ++E Y +LL+ R  L++   E    L E+V+ L++AA R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 KSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQP 165
              L  ++ KE+  +         VN  LM KLS   P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of PI0025973 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.1e-09
Identity = 46/158 (29.11%), Postives = 79/158 (50.00%), Query Frame = 0

Query: 8   LLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRALLRVEKVI 67
           +LG  F+A + R  L L ++RL +L  ++     +A  ++   L      RA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRC-GDFPELHEI 127
           ++   ++A  ++E Y +LL+ R  L++   E    L E+V+ L++AA R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 KSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQP 165
              L  ++ KE+  +         VN  LM KLS   P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of PI0025973 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.1e-09
Identity = 46/158 (29.11%), Postives = 79/158 (50.00%), Query Frame = 0

Query: 8   LLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRALLRVEKVI 67
           +LG  F+A + R  L L ++RL +L  ++     +A  ++   L      RA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRC-GDFPELHEI 127
           ++   ++A  ++E Y +LL+ R  L++   E    L E+V+ L++AA R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 KSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQP 165
              L  ++ KE+  +         VN  LM KLS   P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of PI0025973 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 5.5e-09
Identity = 45/158 (28.48%), Postives = 78/158 (49.37%), Query Frame = 0

Query: 8   LLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRALLRVEKVI 67
           +LG   +A + R  L L ++RL +L  ++     +A  ++   L      RA +RVE +I
Sbjct: 1   MLGSGIKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRC-GDFPELHEI 127
           ++   ++A  ++E Y +LL+ R  L++   E    L E+V+ L++AA R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 KSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQP 165
              L  ++ KE+  +         VN  LM KLS   P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of PI0025973 vs. ExPASy TrEMBL
Match: A0A0A0KAU0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G032470 PE=3 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 4.9e-162
Identity = 330/361 (91.41%), Postives = 338/361 (93.63%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRAL 60
           MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRV  SQANSDVLQLLQLSHHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60

Query: 61  LRVEKVIKDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVEKVIKD+NALDAYVLIEGYLNLL+ERTTLLEQQSECPEELKEAVAGLLFAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASEN 180
           PELHEIKSVLTTRFGKEFTARAVELRNNCGVNL LMQKLSTRQPTLETRM+ LKSIASEN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQPTLETRMDALKSIASEN 180

Query: 181 GILLQIDQLPPSNEEKVARNGRQSEAEGQSEEFSNEVASGSKRTYKDVADAAQAAFESAA 240
           GI+LQIDQLP S +EKV RNGRQSEAEGQSEEFSNEVASGSK TYKDVADAAQAAFESAA
Sbjct: 181 GIVLQIDQLPSSKQEKVGRNGRQSEAEGQSEEFSNEVASGSKTTYKDVADAAQAAFESAA 240

Query: 241 QAAAAARAAMELSRSHEGPGSPSKPGSGTTPHNKQKEEKTEVESQAKQEMEEYRNRKKGE 300
           QAAAAARAAMELSRSHEGP SPSKPGSGTT  NKQK+EK EVES+ KQEMEEY N +K  
Sbjct: 241 QAAAAARAAMELSRSHEGPSSPSKPGSGTTSDNKQKKEKREVESKVKQEMEEYGNGRK-- 300

Query: 301 GEGEGEEEEGGKMDEERTSNTENGLKMETKVEKTEVSEKGSFRLNLEKKPISVRTRRVRG 360
           GEGEGEEEEG  MDEERTS   NGLKMETKVEKTEVSEKGSFRLNLEKKPISVRTRRV G
Sbjct: 301 GEGEGEEEEGDGMDEERTS---NGLKMETKVEKTEVSEKGSFRLNLEKKPISVRTRRVGG 356

Query: 361 Y 362
           Y
Sbjct: 361 Y 356

BLAST of PI0025973 vs. ExPASy TrEMBL
Match: A0A1S3BD83 (uncharacterized protein LOC103488606 OS=Cucumis melo OX=3656 GN=LOC103488606 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.5e-142
Identity = 288/315 (91.43%), Postives = 302/315 (95.87%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRAL 60
           MGRKLDALLGRNFRASKFRPLLNLSLSRLSILT+QRR++ SQANSDVLQLLQLSHHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTSQRRLRCSQANSDVLQLLQLSHHHRAL 60

Query: 61  LRVEKVIKDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVEKVIKD+NALDAYVLIEGYLNLL+ERTTLLEQQSECPEELKEAVAGLLFAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASEN 180
           PELHEIKSVLTTRFGKEFTARAVELRNNCGVNL LMQKLSTRQPTLETRMEVLKSIASEN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQPTLETRMEVLKSIASEN 180

Query: 181 GILLQIDQLPPSNEEKVARNGRQSEAEGQSEEFSNEVASGSKRTYKDVADAAQAAFESAA 240
           GI+LQ DQLP SNEEKVAR+GRQSEAEG+SE+FSNEVAS SK+ YKDVADAAQAAFESAA
Sbjct: 181 GIVLQFDQLPHSNEEKVARSGRQSEAEGRSEKFSNEVASDSKKRYKDVADAAQAAFESAA 240

Query: 241 QAAAAARAAMELSRSHEGPGSPSKPGSGTTPHNKQKEEKTEVESQAKQEMEEYRN--RKK 300
           QAAAAARAAMELSRSHEGP SPSKPGSGTTPH+KQK+EKTEVES+ KQEMEEY N  R +
Sbjct: 241 QAAAAARAAMELSRSHEGPSSPSKPGSGTTPHDKQKKEKTEVESKVKQEMEEYGNGRRGE 300

Query: 301 GEGEGEGEEEEGGKM 314
           GEGEGEGE EEGGKM
Sbjct: 301 GEGEGEGEGEEGGKM 315

BLAST of PI0025973 vs. ExPASy TrEMBL
Match: A0A5A7U1A1 (Putative Regulator of Vps4 activity in the MVB pathway protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold163G00830 PE=3 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.8e-140
Identity = 295/346 (85.26%), Postives = 311/346 (89.88%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRAL 60
           MGRKLDALLGRNFRASKFRPLLNLSLSRLSILT+QRR++ SQANSD              
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTSQRRLRCSQANSD-------------- 60

Query: 61  LRVEKVIKDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
             VEKVIKD+NALDAYVLIEGYLNLL+ERTTLLEQQSECPEELKEAVAGLLFAASRCGDF
Sbjct: 61  --VEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASEN 180
           PELHEIKSVLTTRFGKEFTARAVELRNNCGVNL LMQKLSTRQPTLETRMEVLKSIASEN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQPTLETRMEVLKSIASEN 180

Query: 181 GILLQIDQLPPSNEEKVARNGRQSEAEGQSEEFSNEVASGSKRTYKDVADAAQAAFESAA 240
           GI+LQ DQLP SNEEKVAR+GRQSEAEG+SE+FSNEVAS SK+ YKDVADAAQAAFESAA
Sbjct: 181 GIVLQFDQLPHSNEEKVARSGRQSEAEGRSEKFSNEVASDSKKRYKDVADAAQAAFESAA 240

Query: 241 QAAAAARAAMELSRSHEGPGSPSKPGSGTTPHNKQKEEKTEVESQAKQEMEEYRN--RKK 300
           QAAAAARAAMELSRSHEGP SPSKPGSGTTPH+KQK+EKTEVES+ KQEMEEY N  R +
Sbjct: 241 QAAAAARAAMELSRSHEGPSSPSKPGSGTTPHDKQKKEKTEVESKVKQEMEEYGNGRRGE 300

Query: 301 GEGEGEGEEEEGGK-----MDEERTSNTENGLKMETKVEKTEVSEK 340
           GEGEGEGE EEGGK     MDEERTSNT+NGLKMETKVEKTEV+ K
Sbjct: 301 GEGEGEGEGEEGGKMGGDEMDEERTSNTKNGLKMETKVEKTEVTFK 330

BLAST of PI0025973 vs. ExPASy TrEMBL
Match: A0A6J1E0Z0 (uncharacterized protein LOC111429814 OS=Cucurbita moschata OX=3662 GN=LOC111429814 PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 7.2e-121
Identity = 269/387 (69.51%), Postives = 314/387 (81.14%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRAL 60
           MGR LDALLGRNFRASKFRPLLNL+LSRL+ILT QRR++ SQA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEKVIKDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVE+VIKD+NALDAYVLIEGYLNLL+ERT+LLEQQ +CPEELKEA+AGLLFAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERTSLLEQQRDCPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASEN 180
           PELHEIKSVLT+RFGKEFTARAVELRNNCGVN LLMQKLSTR P LE+RM++LK+IASEN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASEN 180

Query: 181 GILLQIDQLPPSNEEKVARNGRQSEAE-----GQSEEFSNEVASGSKRTYKDVADAAQAA 240
           GI LQ+D+ P SNE   ARN RQ+++E     G++ +FS EV SGSK+ YKDVADAAQAA
Sbjct: 181 GITLQLDETPLSNE---ARNSRQNQSEPHGGVGENLKFSTEVPSGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSH----EGPGSPSKPGSGTTPHNKQKEEKTEVESQAKQEME 300
           FESAAQAAAAARAAMELSRS     +GP SP  PGS TT ++KQ+++K+EVE++ K E+E
Sbjct: 241 FESAAQAAAAARAAMELSRSESQDPDGPSSPG-PGSATTSYHKQEQKKSEVEAKPKSEIE 300

Query: 301 -EYRNRKKG---EGEGEGEE-------------EEGGKMDEERTSNTENGLKMETKVEKT 360
            EY   ++G     E E +              +E  +MDE+R +N E G +ME  +EKT
Sbjct: 301 YEYGKEEEGSKIRAEAEVKNSMCGCSSNRELFVKENREMDEQRATNIEIGEEMEANLEKT 360

Query: 361 EVSEKGSFRLNLEKKPISVRTRRVRGY 362
           E++EK SFRLNLEKKPISVRTRRVRGY
Sbjct: 361 EITEKPSFRLNLEKKPISVRTRRVRGY 383

BLAST of PI0025973 vs. ExPASy TrEMBL
Match: A0A6J1I2V3 (uncharacterized protein LOC111470447 OS=Cucurbita maxima OX=3661 GN=LOC111470447 PE=3 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 7.4e-118
Identity = 264/387 (68.22%), Postives = 310/387 (80.10%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRAL 60
           MGR LDALLGRNFRASKFRPLLNL++SRL+ILT QRR++ +QA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLAVSRLAILTNQRRLRRTQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEKVIKDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVE+VIKD+NALDAYVL+EGYLNLL+ERT+LLEQ+ ECPEELKEA+AGLLFAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLMEGYLNLLIERTSLLEQERECPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASEN 180
           PELHEIKSVLTT FGKEFTARAVELRNNCGVN LLMQKLSTRQP LE+RM++LK+IASEN
Sbjct: 121 PELHEIKSVLTTHFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDLLKTIASEN 180

Query: 181 GILLQIDQLPPSNEEKVARNGRQSEAE-----GQSEEFSNEVASGSKRTYKDVADAAQAA 240
           GI LQ+D+ P SNE   ARN RQ+++E     G++ + S EV SGSK+ YKDVADAAQAA
Sbjct: 181 GITLQLDETPLSNE---ARNSRQNQSEPHGGVGENLKLSTEVPSGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSH----EGPGSPSKPGSGTTPHNKQKEEKTEVESQAKQEME 300
           FESAAQAAAAARAAMELSRS     +GP SP  PGS T+ ++KQ+++K+ VE++ K E+E
Sbjct: 241 FESAAQAAAAARAAMELSRSESQDPDGPSSPG-PGSATSSYDKQEQKKSNVEAKPKLEIE 300

Query: 301 -EYRNRKKGE-------------GEGEGEE---EEGGKMDEERTSNTENGLKMETKVEKT 360
            EY   ++G              G     E   +E  +MDE+R SN E G +ME  +EKT
Sbjct: 301 YEYGREEEGSKIRPKPEVKNSMCGCSSNRELFVKENREMDEQRASNIEIGEEMEANLEKT 360

Query: 361 EVSEKGSFRLNLEKKPISVRTRRVRGY 362
            ++EK SFRLNLEKKPISVRTRRVRGY
Sbjct: 361 AITEKPSFRLNLEKKPISVRTRRVRGY 383

BLAST of PI0025973 vs. NCBI nr
Match: XP_011656519.1 (uncharacterized protein LOC101211044 [Cucumis sativus] >KGN45949.1 hypothetical protein Csa_005294 [Cucumis sativus])

HSP 1 Score: 580.5 bits (1495), Expect = 1.0e-161
Identity = 330/361 (91.41%), Postives = 338/361 (93.63%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRAL 60
           MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRV  SQANSDVLQLLQLSHHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60

Query: 61  LRVEKVIKDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVEKVIKD+NALDAYVLIEGYLNLL+ERTTLLEQQSECPEELKEAVAGLLFAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASEN 180
           PELHEIKSVLTTRFGKEFTARAVELRNNCGVNL LMQKLSTRQPTLETRM+ LKSIASEN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQPTLETRMDALKSIASEN 180

Query: 181 GILLQIDQLPPSNEEKVARNGRQSEAEGQSEEFSNEVASGSKRTYKDVADAAQAAFESAA 240
           GI+LQIDQLP S +EKV RNGRQSEAEGQSEEFSNEVASGSK TYKDVADAAQAAFESAA
Sbjct: 181 GIVLQIDQLPSSKQEKVGRNGRQSEAEGQSEEFSNEVASGSKTTYKDVADAAQAAFESAA 240

Query: 241 QAAAAARAAMELSRSHEGPGSPSKPGSGTTPHNKQKEEKTEVESQAKQEMEEYRNRKKGE 300
           QAAAAARAAMELSRSHEGP SPSKPGSGTT  NKQK+EK EVES+ KQEMEEY N +K  
Sbjct: 241 QAAAAARAAMELSRSHEGPSSPSKPGSGTTSDNKQKKEKREVESKVKQEMEEYGNGRK-- 300

Query: 301 GEGEGEEEEGGKMDEERTSNTENGLKMETKVEKTEVSEKGSFRLNLEKKPISVRTRRVRG 360
           GEGEGEEEEG  MDEERTS   NGLKMETKVEKTEVSEKGSFRLNLEKKPISVRTRRV G
Sbjct: 301 GEGEGEEEEGDGMDEERTS---NGLKMETKVEKTEVSEKGSFRLNLEKKPISVRTRRVGG 356

Query: 361 Y 362
           Y
Sbjct: 361 Y 356

BLAST of PI0025973 vs. NCBI nr
Match: XP_038884116.1 (uncharacterized protein LOC120075040 [Benincasa hispida])

HSP 1 Score: 528.5 bits (1360), Expect = 4.6e-146
Identity = 305/371 (82.21%), Postives = 326/371 (87.87%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRAL 60
           MGRKLDALLGRNFRASKFRPLLNL+LSRLSILTAQRRV+ SQANSDVLQLLQL +HHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLSILTAQRRVRCSQANSDVLQLLQLPNHHRAL 60

Query: 61  LRVEKVIKDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVEKVIKD+NALDAYVLIEGYLNLL+ERTTLLEQQSECPEELKEAVAGLLFAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASEN 180
           PELHEIKSVLTTRFGKEFTARAVELRNNCGVN LLMQKLSTRQPTLE+RMEVLK+IASEN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLESRMEVLKAIASEN 180

Query: 181 GILLQIDQLPPSNEEKVARNGRQSEAEGQSE-----EFSNEVASGSKRTYKDVADAAQAA 240
           GI+LQID  PPSNEEK+ RN RQ++ E +SE     +FS EVASGSK+ YKDVADAAQAA
Sbjct: 181 GIVLQIDNHPPSNEEKLTRNTRQNQPEPRSEVVENLQFSTEVASGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSHEGPGSPSKPGSGTTPHNKQKEEKTEVESQAKQEMEEYRN 300
           FESAAQAAAAARAAMELSRSH+GP SP  PGSGTTPH+KQ+ EK EVES+   E E    
Sbjct: 241 FESAAQAAAAARAAMELSRSHDGPSSPKSPGSGTTPHDKQELEKAEVESKPNPETENRNR 300

Query: 301 RKKGEGEGEGEEEEGGK-----MDEERTSNTENGLKMETKVEKTEVSEKGSFRLNLEKKP 360
           R  G GE E EEEEG K     MDEER S  +NGL +E KVEKTEVSEKGSFRLNLEKKP
Sbjct: 301 RGGGGGEEEEEEEEGNKMRGDEMDEERGSKVKNGLNVERKVEKTEVSEKGSFRLNLEKKP 360

Query: 361 ISVRTRRVRGY 362
           ISVRTRRVRG+
Sbjct: 361 ISVRTRRVRGH 371

BLAST of PI0025973 vs. NCBI nr
Match: XP_008445650.1 (PREDICTED: uncharacterized protein LOC103488606 [Cucumis melo])

HSP 1 Score: 515.8 bits (1327), Expect = 3.1e-142
Identity = 288/315 (91.43%), Postives = 302/315 (95.87%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRAL 60
           MGRKLDALLGRNFRASKFRPLLNLSLSRLSILT+QRR++ SQANSDVLQLLQLSHHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTSQRRLRCSQANSDVLQLLQLSHHHRAL 60

Query: 61  LRVEKVIKDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVEKVIKD+NALDAYVLIEGYLNLL+ERTTLLEQQSECPEELKEAVAGLLFAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASEN 180
           PELHEIKSVLTTRFGKEFTARAVELRNNCGVNL LMQKLSTRQPTLETRMEVLKSIASEN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQPTLETRMEVLKSIASEN 180

Query: 181 GILLQIDQLPPSNEEKVARNGRQSEAEGQSEEFSNEVASGSKRTYKDVADAAQAAFESAA 240
           GI+LQ DQLP SNEEKVAR+GRQSEAEG+SE+FSNEVAS SK+ YKDVADAAQAAFESAA
Sbjct: 181 GIVLQFDQLPHSNEEKVARSGRQSEAEGRSEKFSNEVASDSKKRYKDVADAAQAAFESAA 240

Query: 241 QAAAAARAAMELSRSHEGPGSPSKPGSGTTPHNKQKEEKTEVESQAKQEMEEYRN--RKK 300
           QAAAAARAAMELSRSHEGP SPSKPGSGTTPH+KQK+EKTEVES+ KQEMEEY N  R +
Sbjct: 241 QAAAAARAAMELSRSHEGPSSPSKPGSGTTPHDKQKKEKTEVESKVKQEMEEYGNGRRGE 300

Query: 301 GEGEGEGEEEEGGKM 314
           GEGEGEGE EEGGKM
Sbjct: 301 GEGEGEGEGEEGGKM 315

BLAST of PI0025973 vs. NCBI nr
Match: KAA0049623.1 (putative Regulator of Vps4 activity in the MVB pathway protein [Cucumis melo var. makuwa])

HSP 1 Score: 508.8 bits (1309), Expect = 3.8e-140
Identity = 295/346 (85.26%), Postives = 311/346 (89.88%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRAL 60
           MGRKLDALLGRNFRASKFRPLLNLSLSRLSILT+QRR++ SQANSD              
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTSQRRLRCSQANSD-------------- 60

Query: 61  LRVEKVIKDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
             VEKVIKD+NALDAYVLIEGYLNLL+ERTTLLEQQSECPEELKEAVAGLLFAASRCGDF
Sbjct: 61  --VEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASEN 180
           PELHEIKSVLTTRFGKEFTARAVELRNNCGVNL LMQKLSTRQPTLETRMEVLKSIASEN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQPTLETRMEVLKSIASEN 180

Query: 181 GILLQIDQLPPSNEEKVARNGRQSEAEGQSEEFSNEVASGSKRTYKDVADAAQAAFESAA 240
           GI+LQ DQLP SNEEKVAR+GRQSEAEG+SE+FSNEVAS SK+ YKDVADAAQAAFESAA
Sbjct: 181 GIVLQFDQLPHSNEEKVARSGRQSEAEGRSEKFSNEVASDSKKRYKDVADAAQAAFESAA 240

Query: 241 QAAAAARAAMELSRSHEGPGSPSKPGSGTTPHNKQKEEKTEVESQAKQEMEEYRN--RKK 300
           QAAAAARAAMELSRSHEGP SPSKPGSGTTPH+KQK+EKTEVES+ KQEMEEY N  R +
Sbjct: 241 QAAAAARAAMELSRSHEGPSSPSKPGSGTTPHDKQKKEKTEVESKVKQEMEEYGNGRRGE 300

Query: 301 GEGEGEGEEEEGGK-----MDEERTSNTENGLKMETKVEKTEVSEK 340
           GEGEGEGE EEGGK     MDEERTSNT+NGLKMETKVEKTEV+ K
Sbjct: 301 GEGEGEGEGEEGGKMGGDEMDEERTSNTKNGLKMETKVEKTEVTFK 330

BLAST of PI0025973 vs. NCBI nr
Match: XP_022921602.1 (uncharacterized protein LOC111429814 [Cucurbita moschata] >KAG7032508.1 IST1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 443.7 bits (1140), Expect = 1.5e-120
Identity = 269/387 (69.51%), Postives = 314/387 (81.14%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRAL 60
           MGR LDALLGRNFRASKFRPLLNL+LSRL+ILT QRR++ SQA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEKVIKDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVE+VIKD+NALDAYVLIEGYLNLL+ERT+LLEQQ +CPEELKEA+AGLLFAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERTSLLEQQRDCPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASEN 180
           PELHEIKSVLT+RFGKEFTARAVELRNNCGVN LLMQKLSTR P LE+RM++LK+IASEN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASEN 180

Query: 181 GILLQIDQLPPSNEEKVARNGRQSEAE-----GQSEEFSNEVASGSKRTYKDVADAAQAA 240
           GI LQ+D+ P SNE   ARN RQ+++E     G++ +FS EV SGSK+ YKDVADAAQAA
Sbjct: 181 GITLQLDETPLSNE---ARNSRQNQSEPHGGVGENLKFSTEVPSGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSH----EGPGSPSKPGSGTTPHNKQKEEKTEVESQAKQEME 300
           FESAAQAAAAARAAMELSRS     +GP SP  PGS TT ++KQ+++K+EVE++ K E+E
Sbjct: 241 FESAAQAAAAARAAMELSRSESQDPDGPSSPG-PGSATTSYHKQEQKKSEVEAKPKSEIE 300

Query: 301 -EYRNRKKG---EGEGEGEE-------------EEGGKMDEERTSNTENGLKMETKVEKT 360
            EY   ++G     E E +              +E  +MDE+R +N E G +ME  +EKT
Sbjct: 301 YEYGKEEEGSKIRAEAEVKNSMCGCSSNRELFVKENREMDEQRATNIEIGEEMEANLEKT 360

Query: 361 EVSEKGSFRLNLEKKPISVRTRRVRGY 362
           E++EK SFRLNLEKKPISVRTRRVRGY
Sbjct: 361 EITEKPSFRLNLEKKPISVRTRRVRGY 383

BLAST of PI0025973 vs. TAIR 10
Match: AT1G13340.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 235.3 bits (599), Expect = 7.5e-62
Identity = 173/410 (42.20%), Postives = 237/410 (57.80%), Query Frame = 0

Query: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRAL 60
           MG+KLDALLGR+F+ +KF+ L+ L+L+RLSIL  QR+ + SQA SDV +LL+L  H  A 
Sbjct: 1   MGKKLDALLGRSFKTNKFKSLITLALTRLSILKNQRQARLSQAISDVTELLKLGQHEHAY 60

Query: 61  LRVEKVIKDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
            RV++V+KD+N LD    I GY  L ++R  L E   +CPEEL EAV+GLLFAASR G+F
Sbjct: 61  HRVDQVVKDQNTLDVLFFIHGYFTLCLDRIHLFEHNRDCPEELLEAVSGLLFAASRIGEF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASEN 180
           PEL EI++VL +RFGK+  AR++ELR+NCGV+  ++QKLSTR P  E RM+ LK IA+EN
Sbjct: 121 PELQEIRNVLISRFGKDLAARSIELRSNCGVDPKIIQKLSTRPPPKEVRMKALKEIAAEN 180

Query: 181 GILLQIDQLPPSNEEKVARNG---------RQSEAEGQSEEFSNEVASGSKRTYKDVADA 240
            I+L++DQ   S E      G            +  G+    S+ V  G K+ YKDVADA
Sbjct: 181 NIVLKLDQASTSTEGTTNMQGTSDVSKTKLTSKDGRGEGYGLSDSVKRGKKK-YKDVADA 240

Query: 241 AQAAFESAAQAAAAARAAMELS----RSHEGPGSPSKPGSGTTPHNKQKEEKTEVESQAK 300
           AQAAFESAA AA AA+AA+ELS    R H+ PG+     S     NK+ E++ E    + 
Sbjct: 241 AQAAFESAAHAAEAAQAAVELSQFSPRGHDSPGNIGGENSFHGSENKESEQEQEGNDDSS 300

Query: 301 Q-EMEEYRNRKKGEGEGE--------GEEEEGGKMDEERTSNTENGLKMETKVEKTEVSE 360
           + E +     K+   + E           E+  K+ E+ T   ++  + +  VE   +S+
Sbjct: 301 EGEGDVMSESKRSMSDSEDIIDVPVMSFREDPVKLLEKDTIIYDSEEETQPSVETITISK 360

Query: 361 K--------GSFRLNLE-------------------KKPISVRTRRVRGY 362
                    GS R +                     K P+SVRTR+VRGY
Sbjct: 361 SKDEQNRMVGSNRADTRHVDHMVRSIEDPIMRKAGLKGPVSVRTRQVRGY 409

BLAST of PI0025973 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 117.1 bits (292), Expect = 3.0e-26
Identity = 93/314 (29.62%), Postives = 148/314 (47.13%), Query Frame = 0

Query: 5   LDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRALLRVE 64
           L+ L  R    +K +  LNL+++R+ +L  +R +Q      ++   LQ      A +RVE
Sbjct: 4   LNQLFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVE 63

Query: 65  KVIKDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELH 124
            VI++ N   AY ++E +   ++ R  +LE + ECP EL+EA+A ++FAA RC + P+L 
Sbjct: 64  HVIREMNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLL 123

Query: 125 EIKSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASEN---- 184
           +IK++  T++GKEF   A ELR + GVN  +++KLS   P+   R+++LK IA E     
Sbjct: 124 QIKNLFGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNW 183

Query: 185 -----------------GILLQIDQLPPSNEEKVARNG-RQSEAEGQSEEFSNEVAS--- 244
                            G   QI +    +E + ++ G  QS    + E    E      
Sbjct: 184 DSSATEAEFMKSHEDLLGGAKQIHRQDGISESRPSQQGYGQSSVSREVESLPAEATQRFQ 243

Query: 245 -------------------------GSKRTYKDVADAAQAAFESAAQAAAAARAAMELSR 269
                                     ++R   DV + A+AA  SA +A AAARAA +L  
Sbjct: 244 KLQAQNPVSKSMPSSKLTSAFQAPPDTRRNQTDVMEIARAALASADRATAAARAAAQLVN 303

BLAST of PI0025973 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 107.5 bits (267), Expect = 2.3e-23
Identity = 102/369 (27.64%), Postives = 166/369 (44.99%), Query Frame = 0

Query: 7   ALLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRALLRVEKV 66
           +L  R F +SK +    ++++R+ ++  +R V   Q   D+  LLQ      A +RVE V
Sbjct: 20  SLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHV 79

Query: 67  IKDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELHEI 126
           I+++N   A  +IE +  L+V R T++ +Q +CP +LKE +A L+FAA RC + PEL ++
Sbjct: 80  IREQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDL 139

Query: 127 KSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASENGILLQI 186
           + +   ++GK+F + A +LR +CGVN +L+ KLS R P  E +++++K IA E     Q+
Sbjct: 140 RDIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKE----FQV 199

Query: 187 DQLPPSNEEKVARNGRQS---------------------EAEGQSEEFSNEVASGSKRT- 246
           D      E+++ +   +S                     E    ++      +S S  T 
Sbjct: 200 DWDTTETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTH 259

Query: 247 YKDVADAAQAAFESAAQAAAAARAAMELSRSHEGPGSPSKPGSGTTPHNKQKEEKTEVES 306
           Y D   AA+AA E A QA AAA+ A  L+   +         S  + H K  +       
Sbjct: 260 YHDTESAAEAATELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQYMDHHHH 319

Query: 307 QAKQEMEEYRNRKKGEGEGEGEEEEGGKMDEERTSNTENGLKMETKVEKTEVSEKGSFRL 354
                 +   +         G E  G        +   N    E +   TE   K + R 
Sbjct: 320 HPGSRRQSRDSETSSYYAKPGAENRGMGRRHSYNNPGINESDYEEEYTNTEAEAKETMRR 379

BLAST of PI0025973 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 103.2 bits (256), Expect = 4.4e-22
Identity = 87/312 (27.88%), Postives = 157/312 (50.32%), Query Frame = 0

Query: 5   LDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRALLRVE 64
           LD+   + F+A+K + LL L++ R+ ++  +R  Q  Q   ++ +LL+      A +RVE
Sbjct: 4   LDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVE 63

Query: 65  KVIKDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELH 124
            +I++   + A  ++E +  L+  R  ++E Q ECP +LKEA++ + FAA RC D  EL 
Sbjct: 64  HIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQ 123

Query: 125 EIKSVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASENGILL 184
           +++ +  +++GKEF A A EL+ + GVN  L++ LS R P+ ET++++LK IA E+    
Sbjct: 124 QVQILFVSKYGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEH---- 183

Query: 185 QIDQLPPSNEEKVARNGRQSEAEGQSEEFSNEVASGSKRTY-KDVADAAQAAFESAAQAA 244
           ++D  P S E  + ++  +   +G       +   GSK    ++  +       SAA+  
Sbjct: 184 ELDWDPASTETDLFKS-HEDLLDG-----PKQFGGGSKLPLPEEQNEKTNLTSLSAAKEK 243

Query: 245 AAARAAMELSRSHEGPGSPSKPGSGTTPHNKQKEEKTEVESQAKQEMEEYRNRKKGEGEG 304
           + + +  ++    E P    +P  G T  N     K+        ++         + E 
Sbjct: 244 SDSDSEYDILDFPEVPNVLLRPTPGATSVNAPDAAKSASYEHTSHDL-------PFDSEN 298

Query: 305 EGEEEEGGKMDE 316
            G E+   K DE
Sbjct: 304 AGVEKTASKRDE 298

BLAST of PI0025973 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 100.5 bits (249), Expect = 2.9e-21
Identity = 96/350 (27.43%), Postives = 167/350 (47.71%), Query Frame = 0

Query: 8   LLGRNFRASKFRPLLNLSLSRLSILTAQRRVQFSQANSDVLQLLQLSHHHRALLRVEKVI 67
           +L R F+ +K +  L ++ SRL IL  ++ +Q  Q   ++ QLL+      A +RVE V+
Sbjct: 4   VLQRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVV 63

Query: 68  KDRNALDAYVLIEGYLNLLVERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELHEIK 127
           ++   + AY LI  Y  LLV R  ++E Q  CP +LKEAV  +LFA+ R  D PEL EI 
Sbjct: 64  REEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIF 123

Query: 128 SVLTTRFGKEFTARAVELRNNCGVNLLLMQKLSTRQPTLETRMEVLKSIASENGILLQID 187
              TT++GK+F+  AVELR + GV+ LL++KLS + P   T++++L +IA E+ ++ +  
Sbjct: 124 KQFTTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVWEAQ 183

Query: 188 QLPPSN-EEKVARNGRQSEAEGQSEEFSNEVASGSKRTYKDVADAAQAAFESAAQAAAAA 247
               S+ ++    NG  S     S    + + S  ++     A A   A   +++     
Sbjct: 184 SFVESDPKDTELLNGANSFQPASSMNMDSSINSNKEQPPNIHAPATVNAHHGSSE----- 243

Query: 248 RAAMELSRSHEGPGSPSKPGSGTTPHNKQKEEKTE-------VESQAKQEMEEYRNRKKG 307
                  R H    S +  G  ++  N     K +         S+++ +  E RN   G
Sbjct: 244 -------RHHSPENSYANGGRSSSRSNNVTSGKADDYYHSKARPSRSRPDEGECRNPNHG 303

Query: 308 EGEGEGEEEEGGKMDEERTSNTENGLKMETKVEKTEVSEKGSFRLNLEKK 350
                   ++  K + E   +T+         E+   + + +  L+ +++
Sbjct: 304 YENSSSRNKQ--KWEPEFVDSTDAARAAAEAAERASFAARAAAELSNKER 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54I391.0e-1028.74IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
P539901.1e-0929.11IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1[more]
Q9CX001.1e-0929.11IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1[more]
Q568Z61.1e-0929.11IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1[more]
Q3ZBV15.5e-0928.48IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KAU04.9e-16291.41Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G032470 PE=3 SV=1[more]
A0A1S3BD831.5e-14291.43uncharacterized protein LOC103488606 OS=Cucumis melo OX=3656 GN=LOC103488606 PE=... [more]
A0A5A7U1A11.8e-14085.26Putative Regulator of Vps4 activity in the MVB pathway protein OS=Cucumis melo v... [more]
A0A6J1E0Z07.2e-12169.51uncharacterized protein LOC111429814 OS=Cucurbita moschata OX=3662 GN=LOC1114298... [more]
A0A6J1I2V37.4e-11868.22uncharacterized protein LOC111470447 OS=Cucurbita maxima OX=3661 GN=LOC111470447... [more]
Match NameE-valueIdentityDescription
XP_011656519.11.0e-16191.41uncharacterized protein LOC101211044 [Cucumis sativus] >KGN45949.1 hypothetical ... [more]
XP_038884116.14.6e-14682.21uncharacterized protein LOC120075040 [Benincasa hispida][more]
XP_008445650.13.1e-14291.43PREDICTED: uncharacterized protein LOC103488606 [Cucumis melo][more]
KAA0049623.13.8e-14085.26putative Regulator of Vps4 activity in the MVB pathway protein [Cucumis melo var... [more]
XP_022921602.11.5e-12069.51uncharacterized protein LOC111429814 [Cucurbita moschata] >KAG7032508.1 IST1-lik... [more]
Match NameE-valueIdentityDescription
AT1G13340.17.5e-6242.20Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G25420.13.0e-2629.62Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.12.3e-2327.64Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G34220.24.4e-2227.88Regulator of Vps4 activity in the MVB pathway protein [more]
AT2G19710.12.9e-2127.43Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 272..296
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 189..220
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 247..338
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 271..305
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 312..338
NoneNo IPR availablePANTHERPTHR12161:SF16REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 8..358
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 6..190
e-value: 2.6E-58
score: 198.6
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 21..182
e-value: 5.0E-47
score: 159.9
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 8..358

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0025973.1PI0025973.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport