PI0025821 (gene) Melon (PI 482460) v1

Overview


Name	PI0025821
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Disease resistance protein RGA2-like
Location	chr01: 25975998 .. 25977076 (-)
RNA-Seq Expression	PI0025821
Synteny	PI0025821

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGATTCTATTCTGTTCAACGTTGCTGCAAATGTTATAACCAAAGTGGGCTCTTCTGCAATCCGAGAGCTTGGATTGTTGTGGGGAGTCAATGATGAGCTTGAAAAACTCCAAAACATTCTTTCGACCATTAAAAATGTGCTTCTTGATGCAGAGGAGCAGCAATCCAAGAGCCACGCAGTCAAGGATTGGGTTCAAAAGCTTAAGGATGTTTTCTACGATATTGATGACTTGATTGATGAATTCTCTTATGAAACATTGAGGAGACAAGTTATTACTAAAGATAGAAGAATCATCAAACAAGTACGTAGCTTCATCTCCAAATCTAACCATATTTCATTTGGTCTGAAAATGGGTCAAAAAATTAAACAAATTAGGAAGAAACTAGATGCTATTGCTAATGATAAAACTCAACTCCACCTTTCTGTGCGCATGAGGGAGATACAAGATGATGAGTTGAGGAAGATACGAGAGACTTCCTCTTTTATTCCTGAGGGAGAAGTGATTGGTAGGGATGAAGACAAGAAGACTGTTATAGAATTTTTATTGGATACGAGCACCAAAAAGGATAATGTTGAGGTGGTTTCCATTATTGGCATGGGAGGATTAGGAAAGACCGCACTTGCTCAATCTGTCTATAACGATGAGAAGATAAATAAACATTTTAAGTTGAAATTATGGGTTTGTATCTCTGAAGAATTTAATGTCAAAACAATTGTTAAAAATATAATTGAGTCTATCGAGGAAAAGAAACCTGAGTCTCTTCAATTGGATAAATTACAAAGTATGCTTCAAGAGAAAATTAATGGAAAAAAGTACTTGCTGGTCATGGATGATGTGTGGAATGAAAGCCATGAAAAATGGGTTGGTCTAAAAAGATTTCTAATGGGTGGTGCCAAGGGAAGTAGGATTTTGATCACAACACGTAATCTACAAGTGGCACAGACTTCTGATACAGTTTGGTTTCATCACTTAAAGGTACTTGACAATGACAACTCTTGGGCGTTATTTAGAAAAATGGCATTTTTGAACGAAGAAGAAGAGCTTGAGAATTCAAATTTGGTTAGAATCGGTAA

mRNA sequence

ATGGCAGATTCTATTCTGTTCAACGTTGCTGCAAATGTTATAACCAAAGTGGGCTCTTCTGCAATCCGAGAGCTTGGATTGTTGTGGGGAGTCAATGATGAGCTTGAAAAACTCCAAAACATTCTTTCGACCATTAAAAATGTGCTTCTTGATGCAGAGGAGCAGCAATCCAAGAGCCACGCAGTCAAGGATTGGGTTCAAAAGCTTAAGGATGTTTTCTACGATATTGATGACTTGATTGATGAATTCTCTTATGAAACATTGAGGAGACAAGTTATTACTAAAGATAGAAGAATCATCAAACAAGTACGTAGCTTCATCTCCAAATCTAACCATATTTCATTTGGTCTGAAAATGGGTCAAAAAATTAAACAAATTAGGAAGAAACTAGATGCTATTGCTAATGATAAAACTCAACTCCACCTTTCTGTGCGCATGAGGGAGATACAAGATGATGAGTTGAGGAAGATACGAGAGACTTCCTCTTTTATTCCTGAGGGAGAAGTGATTGGTAGGGATGAAGACAAGAAGACTGTTATAGAATTTTTATTGGATACGAGCACCAAAAAGGATAATGTTGAGGTGAATCGGTAA

Coding sequence (CDS)

ATGGCAGATTCTATTCTGTTCAACGTTGCTGCAAATGTTATAACCAAAGTGGGCTCTTCTGCAATCCGAGAGCTTGGATTGTTGTGGGGAGTCAATGATGAGCTTGAAAAACTCCAAAACATTCTTTCGACCATTAAAAATGTGCTTCTTGATGCAGAGGAGCAGCAATCCAAGAGCCACGCAGTCAAGGATTGGGTTCAAAAGCTTAAGGATGTTTTCTACGATATTGATGACTTGATTGATGAATTCTCTTATGAAACATTGAGGAGACAAGTTATTACTAAAGATAGAAGAATCATCAAACAAGTACGTAGCTTCATCTCCAAATCTAACCATATTTCATTTGGTCTGAAAATGGGTCAAAAAATTAAACAAATTAGGAAGAAACTAGATGCTATTGCTAATGATAAAACTCAACTCCACCTTTCTGTGCGCATGAGGGAGATACAAGATGATGAGTTGAGGAAGATACGAGAGACTTCCTCTTTTATTCCTGAGGGAGAAGTGATTGGTAGGGATGAAGACAAGAAGACTGTTATAGAATTTTTATTGGATACGAGCACCAAAAAGGATAATGTTGAGGTGAATCGGTAA

Protein sequence

MADSILFNVAANVITKVGSSAIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSHAVKDWVQKLKDVFYDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMGQKIKQIRKKLDAIANDKTQLHLSVRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVIEFLLDTSTKKDNVEVNR

Homology

[Close]

BLAST of PI0025821 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 92.4 bits (228), Expect = 6.0e-18
Identity = 58/172 (33.72%), Postives = 99/172 (57.56%), Query Frame = 0


Query: 24  ELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSHAVKDWVQKLKDVFYDIDDLIDEF 83
           EL LL+G  DE ++L ++ STI+ VL DA+E+Q     +++W+QKL    Y++DD++DE+
Sbjct: 20  ELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAATYEVDDILDEY 79

Query: 84  SYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMGQKIKQIRKKLDAIANDKTQLHLS 143
                     TK  R + Q          I F  K+G+++ Q+ KKL+AIA ++ + HL 
Sbjct: 80  K---------TKATRFL-QSEYGRYHPKVIPFRHKVGKRMDQVMKKLNAIAEERKKFHLQ 139

Query: 144 VRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVIEFLLDTSTKKDNVEV 196
            ++ E Q       RET S + E +V GRD++K  +++ L++T++    + V
Sbjct: 140 EKIIERQ----AATRETGSVLTEPQVYGRDKEKDEIVKILINTASDAQKLSV 177

[Close]

BLAST of PI0025821 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 7.8e-18
Identity = 59/195 (30.26%), Postives = 108/195 (55.38%), Query Frame = 0


Query: 1   MADSILFNVAANVITKVGSSAIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSH 60
           MA++ L  +  N+ + +G     +L L++G   E EKL ++ STI+ VL DA+E+Q K  
Sbjct: 1   MAEAFLQVLLENLTSFIGD----KLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDK 60

Query: 61  AVKDWVQKLKDVFYDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMG 120
           A+++W+QKL    Y++DD++ E   E +R           +Q R        I+F  K+G
Sbjct: 61  AIENWLQKLNSAAYEVDDILGECKNEAIR----------FEQSRLGFYHPGIINFRHKIG 120

Query: 121 QKIKQIRKKLDAIANDKTQLHLSVRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVI 180
           +++K+I +KLDAI+ ++ + H   ++ E Q       RET   + E +V GRD+++  ++
Sbjct: 121 RRMKEIMEKLDAISEERRKFHFLEKITERQ--AAAATRETGFVLTEPKVYGRDKEEDEIV 179

Query: 181 EFLLDTSTKKDNVEV 196
           + L++     + + V
Sbjct: 181 KILINNVNVAEELPV 179

[Close]

BLAST of PI0025821 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 89.7 bits (221), Expect = 3.9e-17
Identity = 56/172 (32.56%), Postives = 97/172 (56.40%), Query Frame = 0


Query: 24  ELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSHAVKDWVQKLKDVFYDIDDLIDEF 83
           ELGL++G   E +KL ++ S I+ VL DA+E+Q K  A+K+W+QKL    Y++DD++D+ 
Sbjct: 20  ELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDDC 79

Query: 84  SYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMGQKIKQIRKKLDAIANDKTQLHLS 143
             E  R           KQ          I+F  K+G+++K++ +KLDAIA ++   HL 
Sbjct: 80  KTEAAR----------FKQAVLGRYHPRTITFCYKVGKRMKEMMEKLDAIAEERRNFHLD 139

Query: 144 VRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVIEFLLDTSTKKDNVEV 196
            R+ E Q       R+T   + E +V GR++++  +++ L++  +  + V V
Sbjct: 140 ERIIERQAAR----RQTGFVLTEPKVYGREKEEDEIVKILINNVSYSEEVPV 177

[Close]

BLAST of PI0025821 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-16
Identity = 56/172 (32.56%), Postives = 96/172 (55.81%), Query Frame = 0


Query: 24  ELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSHAVKDWVQKLKDVFYDIDDLIDEF 83
           EL LL+G  DE ++L ++ STI+ VL DA+E+Q  +  +++W+QKL    Y++DD++DE+
Sbjct: 20  ELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAATYEVDDILDEY 79

Query: 84  SYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMGQKIKQIRKKLDAIANDKTQLHLS 143
             +  R       R   K           I F  K+G+++ Q+ KKL AIA ++   HL 
Sbjct: 80  KTKATRFSQSEYGRYHPKV----------IPFRHKVGKRMDQVMKKLKAIAEERKNFHLH 139

Query: 144 VRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVIEFLLDTSTKKDNVEV 196
            ++ E Q       RET S + E +V GRD++K  +++ L++  +   ++ V
Sbjct: 140 EKIVERQAVR----RETGSVLTEPQVYGRDKEKDEIVKILINNVSDAQHLSV 177

[Close]

BLAST of PI0025821 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.4e-11
Identity = 48/164 (29.27%), Postives = 88/164 (53.66%), Query Frame = 0


Query: 35  LEKLQNILSTIKNVLLDAEEQQSKSHAVKDWVQKLKDVFYDIDDLIDEFSYETLRRQV-- 94
           LE+L   L TI  VL+DAEE+Q  +  V+ WV +L+DV Y  +D +D+ + E LR  +  
Sbjct: 39  LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98

Query: 95  ITKDRRIIKQVRSFISKSNHISFGLK-MGQKIKQIRKKLDAIANDKTQLHLSVRMREIQD 154
            +     ++Q+R  +S  + +    + +  +++++  +L+ +A+ +  L L      I  
Sbjct: 99  ESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMIPK 158

Query: 155 DELRKIRETSSFIPEGEVIGRDEDKKTVIEFLLDTSTKKDNVEV 196
             L     T+S + E EV GRD+DK  ++ FL+  + K + + V
Sbjct: 159 QRL----PTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITV 198

[Close]

BLAST of PI0025821 vs. ExPASy TrEMBL
Match: A0A0A0KBE3 (Rx_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G425930 PE=4 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 2.7e-82
Identity = 162/197 (82.23%), Postives = 179/197 (90.86%), Query Frame = 0


Query: 1   MADSILFNVAANVITKVGSSAIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSH 60
           MADSILFNVAANVITK+GSSA+RELG LWGVNDEL KLQNILS IK VLLDAEEQQS SH
Sbjct: 1   MADSILFNVAANVITKLGSSALRELGSLWGVNDELGKLQNILSAIKAVLLDAEEQQSVSH 60

Query: 61  AVKDWVQKLKDVFYDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMG 120
           AVKDW+ KL+DVFYD+DDLIDEFSYETLRRQV+TKDR I KQV  F SKSN +SFG KM 
Sbjct: 61  AVKDWISKLRDVFYDVDDLIDEFSYETLRRQVLTKDRTITKQVCIFFSKSNQVSFGHKMS 120

Query: 121 QKIKQIRKKLDAIANDKTQLHLSVRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVI 180
           QKIKQ+R+KLDAIANDKTQLHLSVRMRE +DDELRK+RET SFIP+GEVIGRD+DKK +I
Sbjct: 121 QKIKQVREKLDAIANDKTQLHLSVRMRETRDDELRKMRETCSFIPKGEVIGRDDDKKAII 180

Query: 181 EFLLDTSTKKDNVEVNR 198
           +FLLDT+T +DNVEVNR
Sbjct: 181 DFLLDTNTMEDNVEVNR 197

[Close]

BLAST of PI0025821 vs. ExPASy TrEMBL
Match: A0A5A7UUA5 (Putative disease resistance protein RGA1 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00790 PE=4 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 1.6e-74
Identity = 150/196 (76.53%), Postives = 174/196 (88.78%), Query Frame = 0


Query: 1   MADSILFNVAANVITKVGSSAIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSH 60
           MADSILFNVAA+VITK+GSSA+RELG LWGVNDEL+KLQN LS IK VLLDAEEQQSKSH
Sbjct: 1   MADSILFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60

Query: 61  AVKDWVQKLKDVFYDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMG 120
            VKDW+ K+KDVFYDIDDLIDEFSYETLRRQV+TKDR I KQVR F SKSN I+FG +MG
Sbjct: 61  TVKDWISKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFEMG 120

Query: 121 QKIKQIRKKLDAIANDKTQLHLSVRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVI 180
           Q IK++R+KLDAIA DK QLHLSV +RE++D+E RK+RETSSFI EGEVIGRDED+K+++
Sbjct: 121 QTIKKVREKLDAIAADKAQLHLSVSVREVRDNEPRKVRETSSFILEGEVIGRDEDRKSIM 180

Query: 181 EFLLDTST-KKDNVEV 196
           +FL +T+   KDNVEV
Sbjct: 181 DFLFNTNNIIKDNVEV 196

[Close]

BLAST of PI0025821 vs. ExPASy TrEMBL
Match: A0A5D3CGN4 (Putative disease resistance protein RGA1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00560 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 6.1e-74
Identity = 149/196 (76.02%), Postives = 173/196 (88.27%), Query Frame = 0


Query: 1   MADSILFNVAANVITKVGSSAIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSH 60
           MADSILFNVAA+VITK+GSSA+RELG LWGVNDEL+KLQN LS IK VLLDAEEQQSKSH
Sbjct: 1   MADSILFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60

Query: 61  AVKDWVQKLKDVFYDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMG 120
            VKDW+ K+KDVFYDIDDLIDEFSYETLRRQV+TKDR   KQVR F SKSN I+FG +MG
Sbjct: 61  TVKDWISKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTTTKQVRIFFSKSNQIAFGFEMG 120

Query: 121 QKIKQIRKKLDAIANDKTQLHLSVRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVI 180
           Q IK++R+KLDAIA DK QLHLSV +RE++D+E RK+RETSSFI EGEVIGRDED+K+++
Sbjct: 121 QTIKKVREKLDAIAADKAQLHLSVSVREVRDNEPRKVRETSSFILEGEVIGRDEDRKSIM 180

Query: 181 EFLLDTST-KKDNVEV 196
           +FL +T+   KDNVEV
Sbjct: 181 DFLFNTNNIIKDNVEV 196

[Close]

BLAST of PI0025821 vs. ExPASy TrEMBL
Match: A0A1S4E353 (putative disease resistance protein RGA4 OS=Cucumis melo OX=3656 GN=LOC107991801 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 6.1e-74
Identity = 149/196 (76.02%), Postives = 173/196 (88.27%), Query Frame = 0


Query: 1   MADSILFNVAANVITKVGSSAIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSH 60
           MADSILFNVAA+VITK+GSSA+RELG LWGVNDEL+KLQN LS IK VLLDAEEQQSKSH
Sbjct: 1   MADSILFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60

Query: 61  AVKDWVQKLKDVFYDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMG 120
            VKDW+ K+KDVFYDIDDLIDEFSYETLRRQV+TKDR   KQVR F SKSN I+FG +MG
Sbjct: 61  TVKDWISKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTTTKQVRIFFSKSNQIAFGFEMG 120

Query: 121 QKIKQIRKKLDAIANDKTQLHLSVRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVI 180
           Q IK++R+KLDAIA DK QLHLSV +RE++D+E RK+RETSSFI EGEVIGRDED+K+++
Sbjct: 121 QTIKKVREKLDAIAADKAQLHLSVSVREVRDNEPRKVRETSSFILEGEVIGRDEDRKSIM 180

Query: 181 EFLLDTST-KKDNVEV 196
           +FL +T+   KDNVEV
Sbjct: 181 DFLFNTNNIIKDNVEV 196

[Close]

BLAST of PI0025821 vs. ExPASy TrEMBL
Match: A0A5A7UTV6 (Disease resistance protein RGA2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00770 PE=4 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.8e-73
Identity = 145/195 (74.36%), Postives = 171/195 (87.69%), Query Frame = 0


Query: 1   MADSILFNVAANVITKVGSSAIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSH 60
           MA+SI+F VA N+ITK+GS A+RELGL W +NDEL+KLQ  LS I+ VLLDAEEQQSKSH
Sbjct: 1   MAESIIFAVAGNMITKMGSLALRELGLSWRINDELDKLQGTLSAIRAVLLDAEEQQSKSH 60

Query: 61  AVKDWVQKLKDVFYDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMG 120
           AVKDW+ KLKDVFYDIDDL+DE SYETL++QV+T+ RR  K VR F SK + ISFGLKMG
Sbjct: 61  AVKDWISKLKDVFYDIDDLVDELSYETLKKQVLTESRRTTKHVRIFFSKFHQISFGLKMG 120

Query: 121 QKIKQIRKKLDAIANDKTQLHLSVRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVI 180
           Q+IK++R+KL+AIA+DK +LHLS+RM EIQ DELRKIRETSSFIPEGEVIGRD+DKK +I
Sbjct: 121 QQIKEVREKLNAIADDKDKLHLSMRMGEIQGDELRKIRETSSFIPEGEVIGRDDDKKVII 180

Query: 181 EFLLDTSTKKDNVEV 196
           +FLLDT TKKDNVEV
Sbjct: 181 DFLLDTKTKKDNVEV 195

[Close]

BLAST of PI0025821 vs. NCBI nr
Match: XP_038897429.1 (putative disease resistance protein RGA4 isoform X2 [Benincasa hispida])

HSP 1 Score: 312.4 bits (799), Expect = 2.8e-81
Identity = 163/195 (83.59%), Postives = 175/195 (89.74%), Query Frame = 0


Query: 1   MADSILFNVAANVITKVGSSAIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSH 60
           MADSILFNVAANVITKVGSSA+REL  LWGVNDE +KLQN LS IK VLLDAEEQQS SH
Sbjct: 1   MADSILFNVAANVITKVGSSALRELASLWGVNDEFDKLQNTLSVIKAVLLDAEEQQSMSH 60

Query: 61  AVKDWVQKLKDVFYDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMG 120
           AVKDW+ KL++VFYDIDDLIDEFSYETLRRQ++TKDRRI KQVR F SKSN ISFG KMG
Sbjct: 61  AVKDWISKLREVFYDIDDLIDEFSYETLRRQILTKDRRITKQVRIFFSKSNQISFGFKMG 120

Query: 121 QKIKQIRKKLDAIANDKTQLHLSVRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVI 180
           QKIKQ+R+KLDAIA DK QLHLSVRMREI D ELRKI ETSSFI EGEVIGRD+DKK +I
Sbjct: 121 QKIKQVREKLDAIAADKAQLHLSVRMREIPDTELRKIPETSSFISEGEVIGRDDDKKIII 180

Query: 181 EFLLDTSTKKDNVEV 196
           +FLLDTSTKKDNVEV
Sbjct: 181 DFLLDTSTKKDNVEV 195

[Close]

BLAST of PI0025821 vs. NCBI nr
Match: XP_038897428.1 (putative disease resistance protein RGA4 isoform X1 [Benincasa hispida])

HSP 1 Score: 312.4 bits (799), Expect = 2.8e-81
Identity = 163/195 (83.59%), Postives = 175/195 (89.74%), Query Frame = 0


Query: 1   MADSILFNVAANVITKVGSSAIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSH 60
           MADSILFNVAANVITKVGSSA+REL  LWGVNDE +KLQN LS IK VLLDAEEQQS SH
Sbjct: 1   MADSILFNVAANVITKVGSSALRELASLWGVNDEFDKLQNTLSVIKAVLLDAEEQQSMSH 60

Query: 61  AVKDWVQKLKDVFYDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMG 120
           AVKDW+ KL++VFYDIDDLIDEFSYETLRRQ++TKDRRI KQVR F SKSN ISFG KMG
Sbjct: 61  AVKDWISKLREVFYDIDDLIDEFSYETLRRQILTKDRRITKQVRIFFSKSNQISFGFKMG 120

Query: 121 QKIKQIRKKLDAIANDKTQLHLSVRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVI 180
           QKIKQ+R+KLDAIA DK QLHLSVRMREI D ELRKI ETSSFI EGEVIGRD+DKK +I
Sbjct: 121 QKIKQVREKLDAIAADKAQLHLSVRMREIPDTELRKIPETSSFISEGEVIGRDDDKKIII 180

Query: 181 EFLLDTSTKKDNVEV 196
           +FLLDTSTKKDNVEV
Sbjct: 181 DFLLDTSTKKDNVEV 195

[Close]

BLAST of PI0025821 vs. NCBI nr
Match: KAE8646390.1 (hypothetical protein Csa_015790 [Cucumis sativus])

HSP 1 Score: 310.5 bits (794), Expect = 1.1e-80
Identity = 160/195 (82.05%), Postives = 177/195 (90.77%), Query Frame = 0


Query: 1   MADSILFNVAANVITKVGSSAIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSH 60
           MADSILFNVAANVITK+GSSA+RELG LWGVNDEL KLQNILS IK VLLDAEEQQS SH
Sbjct: 1   MADSILFNVAANVITKLGSSALRELGSLWGVNDELGKLQNILSAIKAVLLDAEEQQSVSH 60

Query: 61  AVKDWVQKLKDVFYDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMG 120
           AVKDW+ KL+DVFYD+DDLIDEFSYETLRRQV+TKDR I KQV  F SKSN +SFG KM 
Sbjct: 61  AVKDWISKLRDVFYDVDDLIDEFSYETLRRQVLTKDRTITKQVCIFFSKSNQVSFGHKMS 120

Query: 121 QKIKQIRKKLDAIANDKTQLHLSVRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVI 180
           QKIKQ+R+KLDAIANDKTQLHLSVRMRE +DDELRK+RET SFIP+GEVIGRD+DKK +I
Sbjct: 121 QKIKQVREKLDAIANDKTQLHLSVRMRETRDDELRKMRETCSFIPKGEVIGRDDDKKAII 180

Query: 181 EFLLDTSTKKDNVEV 196
           +FLLDT+T +DNVEV
Sbjct: 181 DFLLDTNTMEDNVEV 195

[Close]

BLAST of PI0025821 vs. NCBI nr
Match: XP_011659434.2 (uncharacterized protein LOC101212849 [Cucumis sativus])

HSP 1 Score: 310.5 bits (794), Expect = 1.1e-80
Identity = 160/195 (82.05%), Postives = 177/195 (90.77%), Query Frame = 0


Query: 1    MADSILFNVAANVITKVGSSAIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSH 60
            MADSILFNVAANVITK+GSSA+RELG LWGVNDEL KLQNILS IK VLLDAEEQQS SH
Sbjct: 1541 MADSILFNVAANVITKLGSSALRELGSLWGVNDELGKLQNILSAIKAVLLDAEEQQSVSH 1600

Query: 61   AVKDWVQKLKDVFYDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMG 120
            AVKDW+ KL+DVFYD+DDLIDEFSYETLRRQV+TKDR I KQV  F SKSN +SFG KM 
Sbjct: 1601 AVKDWISKLRDVFYDVDDLIDEFSYETLRRQVLTKDRTITKQVCIFFSKSNQVSFGHKMS 1660

Query: 121  QKIKQIRKKLDAIANDKTQLHLSVRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVI 180
            QKIKQ+R+KLDAIANDKTQLHLSVRMRE +DDELRK+RET SFIP+GEVIGRD+DKK +I
Sbjct: 1661 QKIKQVREKLDAIANDKTQLHLSVRMRETRDDELRKMRETCSFIPKGEVIGRDDDKKAII 1720

Query: 181  EFLLDTSTKKDNVEV 196
            +FLLDT+T +DNVEV
Sbjct: 1721 DFLLDTNTMEDNVEV 1735

[Close]

BLAST of PI0025821 vs. NCBI nr
Match: XP_038897494.1 (putative disease resistance protein RGA4 [Benincasa hispida] >XP_038897495.1 putative disease resistance protein RGA4 [Benincasa hispida] >XP_038897496.1 putative disease resistance protein RGA4 [Benincasa hispida] >XP_038897497.1 putative disease resistance protein RGA4 [Benincasa hispida])

HSP 1 Score: 302.0 bits (772), Expect = 3.8e-78
Identity = 157/195 (80.51%), Postives = 172/195 (88.21%), Query Frame = 0


Query: 1   MADSILFNVAANVITKVGSSAIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSH 60
           MADS++FNVA N+ITK+GSSA+REL  LWGVNDEL+KLQ  LSTIK+VLLDAEEQQS SH
Sbjct: 1   MADSVMFNVATNIITKLGSSALRELVSLWGVNDELDKLQKTLSTIKSVLLDAEEQQSMSH 60

Query: 61  AVKDWVQKLKDVFYDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMG 120
           AVKDW+ KLKD FYDIDDL+DEFSYETLRRQVI KDRRI KQVR F SK NHI FG KMG
Sbjct: 61  AVKDWISKLKDAFYDIDDLVDEFSYETLRRQVIAKDRRITKQVRIFFSKPNHILFGFKMG 120

Query: 121 QKIKQIRKKLDAIANDKTQLHLSVRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVI 180
           +KIK IR+KLDAIA DK  LHLSVRMREI D+ELRKIRE SSFI EGEVIGRD+DKK VI
Sbjct: 121 RKIKGIREKLDAIAADKAHLHLSVRMREIPDNELRKIREISSFILEGEVIGRDDDKKIVI 180

Query: 181 EFLLDTSTKKDNVEV 196
           +FLLDTST+KDNVEV
Sbjct: 181 DFLLDTSTRKDNVEV 195

[Close]

BLAST of PI0025821 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.0e-12
Identity = 48/164 (29.27%), Postives = 88/164 (53.66%), Query Frame = 0


Query: 35  LEKLQNILSTIKNVLLDAEEQQSKSHAVKDWVQKLKDVFYDIDDLIDEFSYETLRRQV-- 94
           LE+L   L TI  VL+DAEE+Q  +  V+ WV +L+DV Y  +D +D+ + E LR  +  
Sbjct: 39  LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98

Query: 95  ITKDRRIIKQVRSFISKSNHISFGLK-MGQKIKQIRKKLDAIANDKTQLHLSVRMREIQD 154
            +     ++Q+R  +S  + +    + +  +++++  +L+ +A+ +  L L      I  
Sbjct: 99  ESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMIPK 158

Query: 155 DELRKIRETSSFIPEGEVIGRDEDKKTVIEFLLDTSTKKDNVEV 196
             L     T+S + E EV GRD+DK  ++ FL+  + K + + V
Sbjct: 159 QRL----PTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITV 198

[Close]

BLAST of PI0025821 vs. TAIR 10
Match: AT1G50180.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.4e-09
Identity = 41/184 (22.28%), Postives = 98/184 (53.26%), Query Frame = 0


Query: 9   VAANVITKVGSSAIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSHAVKDWVQK 68
           + +  + K+G   + E   L+G+ D++++LQ+ L  +   L DA+E+Q +S  V++WV  
Sbjct: 5   IVSVTVQKLGQLLLEEPLFLFGIGDQVKQLQDELKRLNCFLKDADEKQHESERVRNWVAG 64

Query: 69  LKDVFYDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMGQKIKQIRK 128
           +++  YD +D+++ F  +   R+     +R+++++   ++++  +     +G +I++I  
Sbjct: 65  IREASYDAEDILEAFFLKAESRKQ-KGMKRVLRRLACILNEAVSLH---SVGSEIREITS 124

Query: 129 KLDAIANDKTQLHLSVRM-RE--IQDDELRKIRETSSFIPEGEVIGRDEDKKTVIEFLLD 188
           +L  IA       +   M RE     D LR+ R++  ++ E  ++G ++  + ++  L+ 
Sbjct: 125 RLSKIAASMLDFGIKESMGREGLSLSDSLREQRQSFPYVVEHNLVGLEQSLEKLVNDLVS 184

Query: 189 TSTK 190
              K
Sbjct: 185 GGEK 184

[Close]

BLAST of PI0025821 vs. TAIR 10
Match: AT1G53350.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 58.2 bits (139), Expect = 8.9e-09
Identity = 48/188 (25.53%), Postives = 100/188 (53.19%), Query Frame = 0


Query: 14  ITKVGSSAIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSHAVKDWVQKLKDVF 73
           + K+     RE   L G++++++ L+  L  ++++L DA+ +++++  V+++++ +KD+ 
Sbjct: 10  VEKLWELLSRESARLNGIDEQVDGLKRQLGRLQSLLKDADAKKNETERVRNFLEDVKDIV 69

Query: 74  YDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMGQKIKQIRKKLDAI 133
           YD DD+I+ F    LR     K++ I KQVR+    +  +    K    I+ I K++  +
Sbjct: 70  YDADDIIESFLLNELR----GKEKGIKKQVRTL---ACFLVDRRKFASDIEGITKRISEV 129

Query: 134 ANDKTQLHL------SVRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVIEFLLDTS 193
                 L +        R   +Q+ + R+IR+T S   E +++G D+     +E L+D  
Sbjct: 130 IVGMQSLGIQHIADGGGRSLSLQERQ-REIRQTFSRNSESDLVGLDQS----VEELVDHL 185

Query: 194 TKKDNVEV 196
            + D+V+V
Sbjct: 190 VENDSVQV 185

[Close]

BLAST of PI0025821 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 53.5 bits (127), Expect = 2.2e-07
Identity = 45/185 (24.32%), Postives = 90/185 (48.65%), Query Frame = 0


Query: 1   MADSILFNVAANVITKVGSS-AIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKS 60
           MA+S L + A  ++ ++ +S  + EL      +  L++L+  L T   VL DA+++    
Sbjct: 1   MANSYLSSCANVMVERINTSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAEHV 60

Query: 61  HAVKDWVQKLKDVFYDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKM 120
             VK W+  +KD F+  +D++DE   E LRR+V+ +   +    ++ ++    I    K+
Sbjct: 61  REVKHWLTGIKDAFFQAEDILDELQTEALRRRVVAEAGGLGGLFQNLMAGREAIQ--KKI 120

Query: 121 GQKIKQIRKKLDAIANDKTQLHLSVRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTV 180
             K++++ + L+        + L       +    +  R     +P+G ++GR EDK  +
Sbjct: 121 EPKMEKVVRLLEHHVKHIEVIGLKEYSETREPQWRQASRSRPDDLPQGRLVGRVEDKLAL 180

Query: 181 IEFLL 185
           +  LL
Sbjct: 181 VNLLL 183

[Close]

BLAST of PI0025821 vs. TAIR 10
Match: AT1G58410.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 52.0 bits (123), Expect = 6.4e-07
Identity = 42/188 (22.34%), Postives = 93/188 (49.47%), Query Frame = 0


Query: 14  ITKVGSSAIRELGLLWGVNDELEKLQNILSTIKNVLLDAEEQQSKSHAVKDWVQKLKDVF 73
           + K+     +E     GV D++ +L++ L+ +K+ L DA+ ++  S  V+  V+++KD+ 
Sbjct: 8   VEKLWDRLSQEYDQFKGVEDQVTELKSNLNLLKSFLKDADAKKHISEMVRHCVEEIKDIV 67

Query: 74  YDIDDLIDEFSYETLRRQVITKDRRIIKQVRSFISKSNHISFGLKMGQKIKQIRKKLDAI 133
           YD +D+I+ F    + ++ +   R I+K+++ F S    I    ++   I  I K++  +
Sbjct: 68  YDTEDIIETF----ILKEKVEMKRGIMKRIKRFAST---IMDRRELASDIGGISKRISKV 127

Query: 134 ANDKTQLHL------SVRMREIQDDELRKIRETSSFIPEGEVIGRDEDKKTVIEFLLDTS 193
             D     +        R      +  R++R T S   E + +G + + K ++ +L++  
Sbjct: 128 IQDMQSFGVQQIITDGSRSSHPLQERQREMRHTFSRDSENDFVGMEANVKKLVGYLVE-- 184

Query: 194 TKKDNVEV 196
             KD+ ++
Sbjct: 188 --KDDYQI 184

The following BLAST results are available for this feature:

BLAST of PI0025821 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XA42	6.0e-18	33.72	Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R...	[more]
Q7XA39	7.8e-18	30.26	Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R...	[more]
Q7XA40	3.9e-17	32.56	Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R...	[more]
Q7XBQ9	1.5e-16	32.56	Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ...	[more]
Q9LRR4	1.4e-11	29.27	Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of PI0025821 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KBE3	2.7e-82	82.23	Rx_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G425930 PE=4 S...	[more]
A0A5A7UUA5	1.6e-74	76.53	Putative disease resistance protein RGA1 isoform X2 OS=Cucumis melo var. makuwa ...	[more]
A0A5D3CGN4	6.1e-74	76.02	Putative disease resistance protein RGA1 isoform X1 OS=Cucumis melo var. makuwa ...	[more]
A0A1S4E353	6.1e-74	76.02	putative disease resistance protein RGA4 OS=Cucumis melo OX=3656 GN=LOC107991801...	[more]
A0A5A7UTV6	1.8e-73	74.36	Disease resistance protein RGA2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]

BLAST of PI0025821 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897429.1	2.8e-81	83.59	putative disease resistance protein RGA4 isoform X2 [Benincasa hispida]	[more]
XP_038897428.1	2.8e-81	83.59	putative disease resistance protein RGA4 isoform X1 [Benincasa hispida]	[more]
KAE8646390.1	1.1e-80	82.05	hypothetical protein Csa_015790 [Cucumis sativus]	[more]
XP_011659434.2	1.1e-80	82.05	uncharacterized protein LOC101212849 [Cucumis sativus]	[more]
XP_038897494.1	3.8e-78	80.51	putative disease resistance protein RGA4 [Benincasa hispida] >XP_038897495.1 put...	[more]

BLAST of PI0025821 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14470.1	1.0e-12	29.27	NB-ARC domain-containing disease resistance protein	[more]
AT1G50180.1	1.4e-09	22.28	NB-ARC domain-containing disease resistance protein	[more]
AT1G53350.1	8.9e-09	25.53	Disease resistance protein (CC-NBS-LRR class) family	[more]
AT3G14460.1	2.2e-07	24.32	LRR and NB-ARC domains-containing disease resistance protein	[more]
AT1G58410.1	6.4e-07	22.34	Disease resistance protein (CC-NBS-LRR class) family	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 35..55
None	No IPR available	GENE3D	1.20.5.4130		coord: 11..124 e-value: 3.3E-26 score: 93.5
None	No IPR available	PANTHER	PTHR23155:SF1132	OS08G0543100 PROTEIN	coord: 25..187
IPR041118	Rx, N-terminal	PFAM	PF18052	Rx_N	coord: 12..100 e-value: 9.0E-26 score: 90.0
IPR044974	Disease resistance protein, plants	PANTHER	PTHR23155	DISEASE RESISTANCE PROTEIN RP	coord: 25..187
IPR038005	Virus X resistance protein-like, coiled-coil domain	CDD	cd14798	RX-CC_like	coord: 13..135 e-value: 1.77613E-15 score: 67.6471

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0025821.1	PI0025821.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0010960	magnesium ion homeostasis
molecular_function	GO:0043531	ADP binding