PI0025529 (gene) Melon (PI 482460) v1

BLAST of PI0025529 vs. ExPASy Swiss-Prot
Match: A7HYV8 (GTPase Der OS=Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) OX=402881 GN=der PE=3 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.2e-83
Identity = 188/483 (38.92%), Postives = 284/483 (58.80%), Query Frame = 0

Query: 120 VVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLET 179
           V +VGRPNVGKS LFNR + ++ ALV +TP   VTRD REG A+LGDL F ++D++GLE 
Sbjct: 5   VAIVGRPNVGKSTLFNRLVGKKLALVDDTPG--VTRDRREGEARLGDLSFTIIDTAGLEE 64

Query: 180 AASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVVAMN 239
           AA +G++  R    TE  +  + L + LIDARAG+ P D    + LR+    +  ++A N
Sbjct: 65  AA-TGTLEARMRIGTERAIADADLCLLLIDARAGVTPLDKSFSQILRKSPTPV--ILAAN 124

Query: 240 KSESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDN 299
           K E          A  +EA  LG G P+P+SAE G G+ +LY A    L ++   + +D+
Sbjct: 125 KCEGGAGK-----AGRMEAYELGLGAPLPLSAEHGEGLGDLYDA----LAQFAKGLEADD 184

Query: 300 GGLDRFHQVTSSNEDEDT---------QDSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVL 359
            G        +  +D D           D +  L++AI+GRPNVGKSTL+N LL  DR+L
Sbjct: 185 AG-QAVEDALAEEQDADAGFDPDAPYEPDLEAPLRVAIIGRPNVGKSTLVNQLLGEDRML 244

Query: 360 VGPEAGLTRDSIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVA 419
            GPEAG+TRDSI  ++E+ GR + L DTAG   R +  +    LSV  + + +  A VV 
Sbjct: 245 TGPEAGITRDSIGIEWEWRGRRVKLWDTAGMRRRARVTEKLEKLSVADTLRAVRFAEVVV 304

Query: 420 LVLDAEEIARERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILKAVP 479
           ++LDA +        +  ++ IA    +EGR L+++VNK D++      A  +++L+ + 
Sbjct: 305 ILLDATQ------PFERQDLHIADLVEQEGRGLLIVVNKWDMV------AEPQEVLRVLK 364

Query: 480 EEIQTVIPQVTGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLSTARLNRWLRKVMSRH 539
           EE++ ++PQ+ G+P++ +SAL GRG  ++M  +   +  W  R+ TARLNRW+++ +SRH
Sbjct: 365 EELERLLPQIRGVPIVTLSALTGRGTDKLMPAIERVHTFWNARVPTARLNRWMQEAVSRH 424

Query: 540 SWKDLSAQP-KVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKSLKEDFNLGGIPIRI 593
                  +P  +KY +QVK+RPPTF  F S    +  +  R+L   L+E F+L G+PIR+
Sbjct: 425 QPPAAHGRPVNLKYISQVKSRPPTFAVFSSRADDVPTSYRRYLVNGLRETFDLPGVPIRL 460

BLAST of PI0025529 vs. ExPASy Swiss-Prot
Match: A5EI59 (GTPase Der OS=Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) OX=288000 GN=der PE=3 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 7.9e-80
Identity = 184/475 (38.74%), Postives = 266/475 (56.00%), Query Frame = 0

Query: 119 TVVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLE 178
           T+ ++GRPNVGKS LFNR + ++ ALV + P   VTRD REG AKLGDL+F ++D++GL+
Sbjct: 4   TIAIIGRPNVGKSTLFNRLVGQKLALVDDMPG--VTRDRREGEAKLGDLQFTIIDTAGLD 63

Query: 179 TAASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVVAM 238
                GS+  R    TE  +  +    F+IDARAGL P D     + RR   D  +++  
Sbjct: 64  -GGPKGSLTARMQEQTETAIALADALFFVIDARAGLTPADRTFADFARR--ADKPVLLLA 123

Query: 239 NKSESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISD 298
           NKSE             +E+  LG GDP+ ISAE G GM ELY A++             
Sbjct: 124 NKSEGKHGE-----LGAMESYALGLGDPIQISAEHGEGMGELYDALR------------- 183

Query: 299 NGGLDRFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLT 358
            G +         +E+ D + +   +++AIVGRPN GKSTL+N LL  +R+L  PEAG T
Sbjct: 184 -GLVPETEDEVDEHEETDEERAARPIRVAIVGRPNAGKSTLINHLLGEERLLTSPEAGTT 243

Query: 359 RDSIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVLDAEEI 418
           RDSI  + E++GR   + DTAG   R++ E+    LSV  + + +  A VV L++DA+  
Sbjct: 244 RDSIAVEIEYKGRGFRIFDTAGLRRRSRIEEKLEKLSVADALRAVRFAEVVVLMMDAQNR 303

Query: 419 ARERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILKAVPEEIQTVIP 478
             E+      ++ IA     EGRALV+ VNK DLM  K    S      A+  ++   +P
Sbjct: 304 FEEQ------DLRIADLIEREGRALVIAVNKWDLMESKPGQIS------ALRHDVDHWLP 363

Query: 479 QVTGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLSTARLNRWLRKVMSRHSWKDLSAQ 538
           QV+G+P++ +S L G G   +M  +VE+Y  W  R+ TA LNRW  + ++ +    +S +
Sbjct: 364 QVSGVPIVAVSGLMGEGIDRLMQAIVESYAVWNRRVPTAALNRWFEEAIANNPPPAVSGR 423

Query: 539 P-KVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKSLKEDFNLGGIPIRIMQR 593
             K+ Y TQ KARPP+FV F S    +  + +R+L  S++E F L G P+RI  R
Sbjct: 424 RLKLNYITQTKARPPSFVLFCSRADAIPQSYLRYLINSMRETFELPGTPVRITLR 442

BLAST of PI0025529 vs. ExPASy Swiss-Prot
Match: Q2W7M7 (GTPase Der OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=der PE=3 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 6.7e-79
Identity = 192/477 (40.25%), Postives = 274/477 (57.44%), Query Frame = 0

Query: 119 TVVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLE 178
           TV ++GRPNVGKS LFNR + +R A+V++ P   VTRD REG A L  + F V+D++G E
Sbjct: 4   TVAIIGRPNVGKSTLFNRLVGKRLAIVHDLPG--VTRDRREGRASLLGMEFQVVDTAGFE 63

Query: 179 TAASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVVAM 238
                 SI  R    T+  + ++ +A+ LID+RAG+ P D      LRR    + LV   
Sbjct: 64  DDGGD-SIEARMRHQTDMAVAEADVALLLIDSRAGVTPLDRHFADHLRRLPTPVILVA-- 123

Query: 239 NKSESLFDNSCTLLAAGL-EAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVIS 298
           NK E          A GL E+  LG G+PV +SAE G GM EL+ A++    K     ++
Sbjct: 124 NKCEGKAG------APGLYESYGLGMGEPVAVSAEHGEGMYELFEALRDHAIK--AGALT 183

Query: 299 DNGGLDRFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGL 358
             G  D   +  +  ED +  D    L +AIVGRPNVGKSTL N LL  DR+L GPEAGL
Sbjct: 184 PEGD-DPEPEEPAEGEDGE-PDPTRPLTMAIVGRPNVGKSTLGNQLLGQDRLLTGPEAGL 243

Query: 359 TRDSIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVLDAEE 418
           TRD+I  ++E  GR + LVDTAG   + +       LSV  + + +  + VV LV+DA  
Sbjct: 244 TRDAIAVEWEHRGRRMKLVDTAGLRKKAQIYDAIEKLSVGNTIETIRMSEVVVLVMDAAA 303

Query: 419 IARERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILKAVPEEIQTVI 478
           I      +   ++ IAR  VEEGRALV+ +NK D++         +  LK + + ++T +
Sbjct: 304 I------LDKQDLTIARLVVEEGRALVLAINKWDVVDDP------QTALKRLKDRLETSL 363

Query: 479 PQVTGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLSTARLNRWLRKVMSRHSWKDL-- 538
           PQ  G+  + +SAL GRG  ++M  V++T+ KW  R+ TA+LNRWL  ++ RH    L  
Sbjct: 364 PQARGVATVTLSALTGRGIEKLMDAVLDTWTKWNRRIPTAQLNRWLEDMIERHPPPALPG 423

Query: 539 SAQPKVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKSLKEDFNLGGIPIRIMQR 593
             + K++Y TQ KARPPTFV F +   +L ++  R+L   L+E F+L G+P+R+  R
Sbjct: 424 GRRYKIRYMTQAKARPPTFVLFATRPEQLPESYSRYLVNGLREAFDLPGVPVRLYVR 453

BLAST of PI0025529 vs. ExPASy Swiss-Prot
Match: A1B4S0 (GTPase Der OS=Paracoccus denitrificans (strain Pd 1222) OX=318586 GN=der PE=3 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.1e-78
Identity = 188/481 (39.09%), Postives = 278/481 (57.80%), Query Frame = 0

Query: 119 TVVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLE 178
           T+ +VGRPNVGKS LFNR + +R ALV + P   VTRD+REG  +LGDLRF+V+DS+GLE
Sbjct: 4   TLAIVGRPNVGKSTLFNRLVGKRLALVDDQPG--VTRDLREGAGRLGDLRFIVVDSAGLE 63

Query: 179 TAASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVVAM 238
             A   S+  R  R+TE  + ++ + +F+IDAR G+   D      LRR A   ++++A 
Sbjct: 64  -IAEDDSLQGRMRRLTERAVDEADVCLFVIDARVGVTAADEYFADILRRRAR--HVILAA 123

Query: 239 NKSESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISD 298
           NK+E          A  +EA  LG G+P+ ISAE G GM +LY A+ P+ E +  + +  
Sbjct: 124 NKAEGRAGE-----AGAMEAYALGLGEPLRISAEHGEGMDDLYRALVPLAEAFEAQNVQQ 183

Query: 299 NGGLDRFHQVTSSNEDEDTQDSKTK-LQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGL 358
               D   +    +E ED + S++K LQ+A++GRPN GKSTL+N +L  DR+L GPEAG+
Sbjct: 184 APETDVAVE-DGDDETEDWRPSESKPLQIAVIGRPNAGKSTLINKILGEDRLLTGPEAGI 243

Query: 359 TRDSIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVLDAEE 418
           TRDSI     F G  + + DTAG   + +       LSV    + +  A VV ++LD   
Sbjct: 244 TRDSISVSTNFMGTPVRIFDTAGMRKKARVTDKVEKLSVADGLRAVRFAEVVVVLLDV-G 303

Query: 419 IARERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILKAVPEEIQTVI 478
           I  E++ ++     IA  A  EGRA+VV  NK DL   K      EK LK + E  + ++
Sbjct: 304 IPFEQQDLR-----IADFAETEGRAVVVAANKWDLEEDKP-----EK-LKELREAFERLL 363

Query: 479 PQVTGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLSTARLNRWLRKVMSRHSWKDLSA 538
           PQ+ G P++ +SA  G+G   + + +++ +E W  R+ TARLN+WL  +   H       
Sbjct: 364 PQLKGAPLVTVSARTGKGLDRLHNAILKAHEVWNRRIPTARLNQWLSTMTEAHPPPAPGG 423

Query: 539 QP-KVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKSLKEDFNLGGIPIRIMQRVEPK 598
           +  +++Y TQVK RPP FV   +   +L D+  R+L   L++DF++ G PIR+  R +  
Sbjct: 424 RRIRLRYITQVKTRPPAFVVMATHTDKLPDSYQRYLVNGLRQDFDMPGTPIRLTFRDQGT 461

BLAST of PI0025529 vs. ExPASy Swiss-Prot
Match: Q5LR04 (GTPase Der OS=Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) OX=246200 GN=der PE=3 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.3e-77
Identity = 184/488 (37.70%), Postives = 280/488 (57.38%), Query Frame = 0

Query: 119 TVVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLE 178
           T+ +VGRPNVGKS LFNR + +R ALV + P   VTRD+REG A+LGDLRF V+D++GLE
Sbjct: 4   TLAIVGRPNVGKSTLFNRLVGKRLALVDDQPG--VTRDLREGQARLGDLRFTVIDTAGLE 63

Query: 179 TAASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVVAM 238
           T A+  S+  R  R+TE  +  + + +F+IDARAG+ P D      LRR +   ++++A 
Sbjct: 64  T-ATDDSLQGRMRRLTERAVDMADICLFMIDARAGVTPNDEIFADILRRRS--AHVILAA 123

Query: 239 NKSESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISD 298
           NK+E    +     A  +EA  LG G+P+ +SAE G G+ ELY  + P+ ++   +    
Sbjct: 124 NKAEGAAAD-----AGVIEAYGLGLGEPIRLSAEHGEGLNELYAVLMPLADEMEQQAEEQ 183

Query: 299 NGGLDRFHQVTSSNEDEDTQD--------SKTKLQLAIVGRPNVGKSTLLNTLLQSDRVL 358
               D    V    EDED ++         +  LQ+A+VGRPN GKSTL+N +L  DR+L
Sbjct: 184 APETD----VDLDPEDEDGEEVAAPHAITREKPLQVAVVGRPNAGKSTLINRILGEDRLL 243

Query: 359 VGPEAGLTRDSIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVA 418
            GPEAG+TRD+I  Q ++    + + DTAG   + K ++    LSV    + +  A VV 
Sbjct: 244 TGPEAGITRDAISLQIDWNDTPMRIFDTAGMRKKAKVQEKLEKLSVSDGLRAVKFAEVVV 303

Query: 419 LVLDAEEIARERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILKAVP 478
           ++LDA  I  E++ ++     IA  A  EGRA+V+ VNK D+   K++       L+ + 
Sbjct: 304 VLLDA-AIPFEQQDLR-----IADLAEREGRAVVIAVNKWDVEENKQD------KLRELK 363

Query: 479 EEIQTVIPQVTGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLSTARLNRWLRKVMSRH 538
           E  + ++PQ+ G P++ +SA  GRG   +   ++  +E W  R+ TA LNRWL  ++ +H
Sbjct: 364 ESFERLLPQLRGAPLVTVSAKTGRGLERLHDAILRAHEVWNRRIPTAALNRWLIGMLEQH 423

Query: 539 -SWKDLSAQPKVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKSLKEDFNLGGIPIRI 598
                   + K++Y TQ K RPP FV   S   ++ ++  R+L   L+ DF++ G PIR+
Sbjct: 424 PPPAPQGKRIKLRYMTQAKTRPPGFVVMCSHPDKMPESYSRYLVNGLRADFDMPGTPIRL 465

BLAST of PI0025529 vs. ExPASy TrEMBL
Match: A0A1S3BT83 (GTP-binding protein EngA OS=Cucumis melo OX=3656 GN=LOC103493287 PE=3 SV=1)

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 573/601 (95.34%), Postives = 582/601 (96.84%), Query Frame = 0

Query: 1   MSRLWVPLLSQRRRFIGNGVFHIVETSRRICSRTPSPNFLTDLVFVKSGWNTCISTARLL 60
           MSRLW PLLSQRRRFIGNGVFH VETSRRICS TPSPN L+D VFVKSGWNTCIS  RLL
Sbjct: 1   MSRLWAPLLSQRRRFIGNGVFHFVETSRRICSSTPSPNLLSDSVFVKSGWNTCISNTRLL 60

Query: 61  LPLPAVHSARGFCGVPENGLSEKCLNDSASDGKVVANKVQGVQRKSVDFTKIPINMLPTV 120
           LPL AVH ARGFCGVPENGLSEKCLNDSASDGKVVA KV+GVQR SVDFTKIPINMLPTV
Sbjct: 61  LPLAAVHIARGFCGVPENGLSEKCLNDSASDGKVVAKKVRGVQRNSVDFTKIPINMLPTV 120

Query: 121 VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA 180
           VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA
Sbjct: 121 VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA 180

Query: 181 ASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVVAMNK 240
           ASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRH PDINLVVAMNK
Sbjct: 181 ASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHTPDINLVVAMNK 240

Query: 241 SESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDNG 300
           SESLFDNSCTLLAAG EAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDNG
Sbjct: 241 SESLFDNSCTLLAAGFEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDNG 300

Query: 301 GLDRFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRD 360
           GLD FHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQS+RVLVGPEAGLTRD
Sbjct: 301 GLDCFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSNRVLVGPEAGLTRD 360

Query: 361 SIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVLDAEEIAR 420
           SIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSV+QS KNLMRAHVVALVLDAEEIAR
Sbjct: 361 SIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVVQSKKNLMRAHVVALVLDAEEIAR 420

Query: 421 ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILKAVPEEIQTVIPQV 480
           ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKIL+AVPEEIQTVIPQV
Sbjct: 421 ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILEAVPEEIQTVIPQV 480

Query: 481 TGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLSTARLNRWLRKVMSRHSWKDLSAQPK 540
           TGIPVIFISALEGRGRLEVMHQV+ETYEKWC RLSTARLNRWLRKVMSRHSWKD SAQPK
Sbjct: 481 TGIPVIFISALEGRGRLEVMHQVIETYEKWCTRLSTARLNRWLRKVMSRHSWKDQSAQPK 540

Query: 541 VKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKSLKEDFNLGGIPIRIMQRVEPKKTVD 600
           VKYFTQVKARPPTFVAFVSGK +LSDTDIRFLTKSLKEDFNLGGIP+RIMQRV PK+TVD
Sbjct: 541 VKYFTQVKARPPTFVAFVSGKKQLSDTDIRFLTKSLKEDFNLGGIPLRIMQRVVPKRTVD 600

Query: 601 G 602
           G
Sbjct: 601 G 601

BLAST of PI0025529 vs. ExPASy TrEMBL
Match: A0A5A7V421 (GTP-binding protein EngA OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22G003240 PE=3 SV=1)

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 573/601 (95.34%), Postives = 583/601 (97.00%), Query Frame = 0

Query: 1   MSRLWVPLLSQRRRFIGNGVFHIVETSRRICSRTPSPNFLTDLVFVKSGWNTCISTARLL 60
           MSRLW PLLSQRRRFIGNGVFH VETSRRICS TPSPN L+D VFVKSGWNTCIS  RLL
Sbjct: 1   MSRLWAPLLSQRRRFIGNGVFHFVETSRRICSSTPSPNLLSDSVFVKSGWNTCISNTRLL 60

Query: 61  LPLPAVHSARGFCGVPENGLSEKCLNDSASDGKVVANKVQGVQRKSVDFTKIPINMLPTV 120
           LPL AVH ARGFCGVPENGLSEKCLNDSASDGKVVA KV+GVQR SVDFTKIPINMLPTV
Sbjct: 61  LPLAAVHIARGFCGVPENGLSEKCLNDSASDGKVVAKKVRGVQRNSVDFTKIPINMLPTV 120

Query: 121 VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA 180
           VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA
Sbjct: 121 VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA 180

Query: 181 ASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVVAMNK 240
           ASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRH PDINLVVAMNK
Sbjct: 181 ASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHTPDINLVVAMNK 240

Query: 241 SESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDNG 300
           SESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDNG
Sbjct: 241 SESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDNG 300

Query: 301 GLDRFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRD 360
           GLD FHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQS+RVLVGPEAGLTRD
Sbjct: 301 GLDCFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSNRVLVGPEAGLTRD 360

Query: 361 SIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVLDAEEIAR 420
           SIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSV+QS KNLMRAHVVALVLDAEEIAR
Sbjct: 361 SIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVVQSKKNLMRAHVVALVLDAEEIAR 420

Query: 421 ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILKAVPEEIQTVIPQV 480
           ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKIL+AVPEEIQTVIPQV
Sbjct: 421 ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILEAVPEEIQTVIPQV 480

Query: 481 TGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLSTARLNRWLRKVMSRHSWKDLSAQPK 540
           TGIPVIFISALEGRGRLEVMHQV+ETYEKWC RLSTARLNRWLRKVMSRHSWKD SAQPK
Sbjct: 481 TGIPVIFISALEGRGRLEVMHQVIETYEKWCTRLSTARLNRWLRKVMSRHSWKDQSAQPK 540

Query: 541 VKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKSLKEDFNLGGIPIRIMQRVEPKKTVD 600
           VKYFTQVKARPPTFVAFVSGK +LSDTDIRFLTKSLKEDFNLGGIP+RIMQRV PK+TV+
Sbjct: 541 VKYFTQVKARPPTFVAFVSGKKQLSDTDIRFLTKSLKEDFNLGGIPLRIMQRVVPKRTVN 600

Query: 601 G 602
           G
Sbjct: 601 G 601

BLAST of PI0025529 vs. ExPASy TrEMBL
Match: A0A0A0L3E8 (GTP-binding protein EngA OS=Cucumis sativus OX=3659 GN=Csa_3G113350 PE=3 SV=1)

HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 567/601 (94.34%), Postives = 580/601 (96.51%), Query Frame = 0

Query: 1   MSRLWVPLLSQRRRFIGNGVFHIVETSRRICSRTPSPNFLTDLVFVKSGWNTCISTARLL 60
           MSRLWVPLLSQRRRFI N VFHIVE+SRRICS TPSPN L+D VF++SGWNT IS ARLL
Sbjct: 1   MSRLWVPLLSQRRRFIPNRVFHIVESSRRICSSTPSPNLLSDSVFLESGWNTRISNARLL 60

Query: 61  LPLPAVHSARGFCGVPENGLSEKCLNDSASDGKVVANKVQGVQRKSVDFTKIPINMLPTV 120
           LPL AVH ARG+CGVPENGL EK LNDSASDGKVVANKVQGVQR SVDF KIPINMLPTV
Sbjct: 61  LPLAAVHIARGYCGVPENGLPEKRLNDSASDGKVVANKVQGVQRNSVDFKKIPINMLPTV 120

Query: 121 VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA 180
           VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLET 
Sbjct: 121 VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETT 180

Query: 181 ASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVVAMNK 240
           ASSGSILERTA+MTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAP+INLVVAMNK
Sbjct: 181 ASSGSILERTAKMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPNINLVVAMNK 240

Query: 241 SESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDNG 300
           SESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELY AIKPVLEKYMLKVISDNG
Sbjct: 241 SESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYGAIKPVLEKYMLKVISDNG 300

Query: 301 GLDRFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRD 360
           G DRFHQVT+SNED+DT DSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRD
Sbjct: 301 GRDRFHQVTNSNEDDDTLDSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRD 360

Query: 361 SIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVLDAEEIAR 420
           SIRA+FEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQS+KNLMRAHVVALVLDAEEIAR
Sbjct: 361 SIRAEFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSTKNLMRAHVVALVLDAEEIAR 420

Query: 421 ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILKAVPEEIQTVIPQV 480
           ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKIL+AVPEEIQTVIPQV
Sbjct: 421 ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILEAVPEEIQTVIPQV 480

Query: 481 TGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLSTARLNRWLRKVMSRHSWKDLSAQPK 540
           TGIPVIFISALEGRGRLEVMHQV+ETYEKWC RLSTARLNRWLRKVMSRHSWKD SAQPK
Sbjct: 481 TGIPVIFISALEGRGRLEVMHQVIETYEKWCTRLSTARLNRWLRKVMSRHSWKDQSAQPK 540

Query: 541 VKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKSLKEDFNLGGIPIRIMQRVEPKKTVD 600
           VKYFTQVKARPPTFVAFVSGK RLSDTDIRFLTKSLKEDFNLGGIPIRIMQRV PKKTVD
Sbjct: 541 VKYFTQVKARPPTFVAFVSGKIRLSDTDIRFLTKSLKEDFNLGGIPIRIMQRVVPKKTVD 600

Query: 601 G 602
           G
Sbjct: 601 G 601

BLAST of PI0025529 vs. ExPASy TrEMBL
Match: A0A6J1J3J0 (GTP-binding protein EngA OS=Cucurbita maxima OX=3661 GN=LOC111483067 PE=3 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 1.9e-294
Identity = 532/626 (84.98%), Postives = 553/626 (88.34%), Query Frame = 0

Query: 1   MSRLWVPLLSQRRRFIGNGVFHIVETSRRICSRTPSPNFLTDLV---------------- 60
           MSRLW PLLSQRRR IGNGV  IV  SR     +  PNFL+  V                
Sbjct: 1   MSRLWFPLLSQRRRVIGNGVSDIVGRSRTTSFSSLLPNFLSGSVLCNGGHTPSNFSTLPK 60

Query: 61  ---------FVKSGWNTCISTARLLLPLPAVHSARGFCGVPENGLSEKCLNDSASDGKVV 120
                    FVKSGWN+ IS+ RLLLPL AVH ARGFCGV ENG SEK LND ASDG VV
Sbjct: 61  PLSEKCNHSFVKSGWNSGISSTRLLLPLAAVHIARGFCGVSENGHSEKFLNDKASDGDVV 120

Query: 121 ANKVQGVQRKSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTR 180
           AN VQ V + SVDFTKIPINMLPTVVLVGRPNVGKSALFNRFI+RREALVYNTPDDHVTR
Sbjct: 121 ANIVQAVHKNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180

Query: 181 DIREGVAKLGDLRFVVLDSSGLETAASSGSILERTARMTENVLLKSQLAIFLIDARAGLH 240
           DIREGVAKLGDLRFVVLDSSGLETA SSGSILERTARMTE VLLK QLAIFLIDARAGLH
Sbjct: 181 DIREGVAKLGDLRFVVLDSSGLETAVSSGSILERTARMTEKVLLKCQLAIFLIDARAGLH 240

Query: 241 PFDLEVGKWLRRHAPDINLVVAMNKSESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGL 300
           PFDLEVGKWLRR+ PDIN++VAMNKSESL D+S TLLAAG EA+RLGFGDPVPISAETGL
Sbjct: 241 PFDLEVGKWLRRNVPDINILVAMNKSESLIDSSGTLLAAGFEAERLGFGDPVPISAETGL 300

Query: 301 GMQELYWAIKPVLEKYMLKVISDNGGLDRFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVG 360
           GM +LY AIKPVLEKYMLKVI+DNGGLDRFHQVT SNEDEDTQDSK +LQLAIVGRPNVG
Sbjct: 301 GMHDLYLAIKPVLEKYMLKVINDNGGLDRFHQVTGSNEDEDTQDSKVQLQLAIVGRPNVG 360

Query: 361 KSTLLNTLLQSDRVLVGPEAGLTRDSIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLS 420
           KSTLLNTLLQSDRVLVGPEAGLTRDS+RAQFEFEGRTIYLVDTAGWLHRTKEEKGP SLS
Sbjct: 361 KSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPSSLS 420

Query: 421 VMQSSKNLMRAHVVALVLDAEEIARERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRG 480
           VMQS+KNLMRAHVVALVLDAEEIARERRSMKH+EVVIARRAVEEGR LV+IVNKMDLMRG
Sbjct: 421 VMQSTKNLMRAHVVALVLDAEEIARERRSMKHAEVVIARRAVEEGRGLVIIVNKMDLMRG 480

Query: 481 KKNFASYEKILKAVPEEIQTVIPQVTGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLS 540
           +KN ASYEKIL+AVPEEIQTVIPQVTGIPVIFISALEGRGRL VM QVVETYEKWC+RLS
Sbjct: 481 RKNSASYEKILEAVPEEIQTVIPQVTGIPVIFISALEGRGRLAVMRQVVETYEKWCLRLS 540

Query: 541 TARLNRWLRKVMSRHSWKDLSAQPKVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKS 600
           TARLNRWLRKVMSRHSWKD SAQPKVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKS
Sbjct: 541 TARLNRWLRKVMSRHSWKDQSAQPKVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKS 600

Query: 601 LKEDFNLGGIPIRIMQRVEPKKTVDG 602
           LKEDFNLGGIPIRIMQR  PKKT DG
Sbjct: 601 LKEDFNLGGIPIRIMQRAVPKKTDDG 626

BLAST of PI0025529 vs. ExPASy TrEMBL
Match: A0A6J1C8F6 (GTP-binding protein EngA OS=Momordica charantia OX=3673 GN=LOC111008384 PE=3 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 1.2e-291
Identity = 528/626 (84.35%), Postives = 547/626 (87.38%), Query Frame = 0

Query: 1   MSRLWVPLLSQRRRFIGNGVFHIVETSRRICSRTPSPNFLTDLV---------------- 60
           MSRLWVPLLS RRR IG GVF I   SRR  S T +PNF +  V                
Sbjct: 1   MSRLWVPLLSHRRRVIGYGVFRIGGRSRRTSSSTVTPNFFSGSVFCSEGHTSSKFSSLPE 60

Query: 61  ---------FVKSGWNTCISTARLLLPLPAVHSARGFCGVPENGLSEKCLNDSASDGKVV 120
                    FV SGWN+CIS  RLL+PL A   ARGFCG+ ENG SEK  ND+A D  V 
Sbjct: 61  PWSEKCNHSFVTSGWNSCISNPRLLVPLAAARIARGFCGLSENGNSEKRSNDNAMDSDVS 120

Query: 121 ANKVQGVQRKSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTR 180
            NKVQGV R SVDFTKI I+MLPTVVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTR
Sbjct: 121 GNKVQGVHRNSVDFTKISISMLPTVVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTR 180

Query: 181 DIREGVAKLGDLRFVVLDSSGLETAASSGSILERTARMTENVLLKSQLAIFLIDARAGLH 240
           DIREGVAKLGDLRFVVLDSSGLET ASSGSILERTARMT NVLLKSQLAIFLIDARAGLH
Sbjct: 181 DIREGVAKLGDLRFVVLDSSGLETTASSGSILERTARMTGNVLLKSQLAIFLIDARAGLH 240

Query: 241 PFDLEVGKWLRRHAPDINLVVAMNKSESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGL 300
           PFDLEVGKWLRRH PDI ++VAMNKSESLFD+S TLLAA LEAQRLGFGDPVPISAETGL
Sbjct: 241 PFDLEVGKWLRRHTPDIKIIVAMNKSESLFDSSGTLLAAALEAQRLGFGDPVPISAETGL 300

Query: 301 GMQELYWAIKPVLEKYMLKVISDNGGLDRFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVG 360
           GMQELY AIKPVLE YMLKVI DNGGLDRFHQVTSSNEDEDTQDSK  LQLAIVGRPNVG
Sbjct: 301 GMQELYCAIKPVLENYMLKVIDDNGGLDRFHQVTSSNEDEDTQDSKLPLQLAIVGRPNVG 360

Query: 361 KSTLLNTLLQSDRVLVGPEAGLTRDSIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLS 420
           KSTLLNTLLQSDRVLVGPEAGLTRDS+RAQF+FE RTIYLVDTAGWLHRTKEEKGP SLS
Sbjct: 361 KSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFQFEDRTIYLVDTAGWLHRTKEEKGPASLS 420

Query: 421 VMQSSKNLMRAHVVALVLDAEEIARERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRG 480
           VMQSSKNLMRAHVVALVLDAEEIARERRSMKH+EVVIARRAVEEGR LVVIVNKMDLMRG
Sbjct: 421 VMQSSKNLMRAHVVALVLDAEEIARERRSMKHAEVVIARRAVEEGRGLVVIVNKMDLMRG 480

Query: 481 KKNFASYEKILKAVPEEIQTVIPQVTGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLS 540
           K+NFASYEKIL+AVPEEIQTVIPQVTGIPVIFISALEGRGRL VM QV+ETYEKWC RLS
Sbjct: 481 KENFASYEKILEAVPEEIQTVIPQVTGIPVIFISALEGRGRLAVMRQVIETYEKWCSRLS 540

Query: 541 TARLNRWLRKVMSRHSWKDLSAQPKVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKS 600
           TARLNRWLRKVMSRHSWKD SAQPKVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKS
Sbjct: 541 TARLNRWLRKVMSRHSWKDQSAQPKVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKS 600

Query: 601 LKEDFNLGGIPIRIMQRVEPKKTVDG 602
           LK+DF+L GIPIRIMQR  PKK+VDG
Sbjct: 601 LKDDFDLVGIPIRIMQRAVPKKSVDG 626

BLAST of PI0025529 vs. NCBI nr
Match: XP_008452192.1 (PREDICTED: GTPase Der isoform X1 [Cucumis melo])

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 573/601 (95.34%), Postives = 582/601 (96.84%), Query Frame = 0

Query: 1   MSRLWVPLLSQRRRFIGNGVFHIVETSRRICSRTPSPNFLTDLVFVKSGWNTCISTARLL 60
           MSRLW PLLSQRRRFIGNGVFH VETSRRICS TPSPN L+D VFVKSGWNTCIS  RLL
Sbjct: 1   MSRLWAPLLSQRRRFIGNGVFHFVETSRRICSSTPSPNLLSDSVFVKSGWNTCISNTRLL 60

Query: 61  LPLPAVHSARGFCGVPENGLSEKCLNDSASDGKVVANKVQGVQRKSVDFTKIPINMLPTV 120
           LPL AVH ARGFCGVPENGLSEKCLNDSASDGKVVA KV+GVQR SVDFTKIPINMLPTV
Sbjct: 61  LPLAAVHIARGFCGVPENGLSEKCLNDSASDGKVVAKKVRGVQRNSVDFTKIPINMLPTV 120

Query: 121 VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA 180
           VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA
Sbjct: 121 VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA 180

Query: 181 ASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVVAMNK 240
           ASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRH PDINLVVAMNK
Sbjct: 181 ASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHTPDINLVVAMNK 240

Query: 241 SESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDNG 300
           SESLFDNSCTLLAAG EAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDNG
Sbjct: 241 SESLFDNSCTLLAAGFEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDNG 300

Query: 301 GLDRFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRD 360
           GLD FHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQS+RVLVGPEAGLTRD
Sbjct: 301 GLDCFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSNRVLVGPEAGLTRD 360

Query: 361 SIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVLDAEEIAR 420
           SIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSV+QS KNLMRAHVVALVLDAEEIAR
Sbjct: 361 SIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVVQSKKNLMRAHVVALVLDAEEIAR 420

Query: 421 ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILKAVPEEIQTVIPQV 480
           ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKIL+AVPEEIQTVIPQV
Sbjct: 421 ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILEAVPEEIQTVIPQV 480

Query: 481 TGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLSTARLNRWLRKVMSRHSWKDLSAQPK 540
           TGIPVIFISALEGRGRLEVMHQV+ETYEKWC RLSTARLNRWLRKVMSRHSWKD SAQPK
Sbjct: 481 TGIPVIFISALEGRGRLEVMHQVIETYEKWCTRLSTARLNRWLRKVMSRHSWKDQSAQPK 540

Query: 541 VKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKSLKEDFNLGGIPIRIMQRVEPKKTVD 600
           VKYFTQVKARPPTFVAFVSGK +LSDTDIRFLTKSLKEDFNLGGIP+RIMQRV PK+TVD
Sbjct: 541 VKYFTQVKARPPTFVAFVSGKKQLSDTDIRFLTKSLKEDFNLGGIPLRIMQRVVPKRTVD 600

Query: 601 G 602
           G
Sbjct: 601 G 601

BLAST of PI0025529 vs. NCBI nr
Match: KAA0060489.1 (GTPase Der isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 573/601 (95.34%), Postives = 583/601 (97.00%), Query Frame = 0

Query: 1   MSRLWVPLLSQRRRFIGNGVFHIVETSRRICSRTPSPNFLTDLVFVKSGWNTCISTARLL 60
           MSRLW PLLSQRRRFIGNGVFH VETSRRICS TPSPN L+D VFVKSGWNTCIS  RLL
Sbjct: 1   MSRLWAPLLSQRRRFIGNGVFHFVETSRRICSSTPSPNLLSDSVFVKSGWNTCISNTRLL 60

Query: 61  LPLPAVHSARGFCGVPENGLSEKCLNDSASDGKVVANKVQGVQRKSVDFTKIPINMLPTV 120
           LPL AVH ARGFCGVPENGLSEKCLNDSASDGKVVA KV+GVQR SVDFTKIPINMLPTV
Sbjct: 61  LPLAAVHIARGFCGVPENGLSEKCLNDSASDGKVVAKKVRGVQRNSVDFTKIPINMLPTV 120

Query: 121 VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA 180
           VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA
Sbjct: 121 VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA 180

Query: 181 ASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVVAMNK 240
           ASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRH PDINLVVAMNK
Sbjct: 181 ASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHTPDINLVVAMNK 240

Query: 241 SESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDNG 300
           SESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDNG
Sbjct: 241 SESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDNG 300

Query: 301 GLDRFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRD 360
           GLD FHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQS+RVLVGPEAGLTRD
Sbjct: 301 GLDCFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSNRVLVGPEAGLTRD 360

Query: 361 SIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVLDAEEIAR 420
           SIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSV+QS KNLMRAHVVALVLDAEEIAR
Sbjct: 361 SIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVVQSKKNLMRAHVVALVLDAEEIAR 420

Query: 421 ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILKAVPEEIQTVIPQV 480
           ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKIL+AVPEEIQTVIPQV
Sbjct: 421 ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILEAVPEEIQTVIPQV 480

Query: 481 TGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLSTARLNRWLRKVMSRHSWKDLSAQPK 540
           TGIPVIFISALEGRGRLEVMHQV+ETYEKWC RLSTARLNRWLRKVMSRHSWKD SAQPK
Sbjct: 481 TGIPVIFISALEGRGRLEVMHQVIETYEKWCTRLSTARLNRWLRKVMSRHSWKDQSAQPK 540

Query: 541 VKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKSLKEDFNLGGIPIRIMQRVEPKKTVD 600
           VKYFTQVKARPPTFVAFVSGK +LSDTDIRFLTKSLKEDFNLGGIP+RIMQRV PK+TV+
Sbjct: 541 VKYFTQVKARPPTFVAFVSGKKQLSDTDIRFLTKSLKEDFNLGGIPLRIMQRVVPKRTVN 600

Query: 601 G 602
           G
Sbjct: 601 G 601

BLAST of PI0025529 vs. NCBI nr
Match: XP_004134261.1 (uncharacterized protein LOC101216538 isoform X1 [Cucumis sativus] >KGN56293.1 hypothetical protein Csa_011260 [Cucumis sativus])

HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 567/601 (94.34%), Postives = 580/601 (96.51%), Query Frame = 0

Query: 1   MSRLWVPLLSQRRRFIGNGVFHIVETSRRICSRTPSPNFLTDLVFVKSGWNTCISTARLL 60
           MSRLWVPLLSQRRRFI N VFHIVE+SRRICS TPSPN L+D VF++SGWNT IS ARLL
Sbjct: 1   MSRLWVPLLSQRRRFIPNRVFHIVESSRRICSSTPSPNLLSDSVFLESGWNTRISNARLL 60

Query: 61  LPLPAVHSARGFCGVPENGLSEKCLNDSASDGKVVANKVQGVQRKSVDFTKIPINMLPTV 120
           LPL AVH ARG+CGVPENGL EK LNDSASDGKVVANKVQGVQR SVDF KIPINMLPTV
Sbjct: 61  LPLAAVHIARGYCGVPENGLPEKRLNDSASDGKVVANKVQGVQRNSVDFKKIPINMLPTV 120

Query: 121 VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA 180
           VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLET 
Sbjct: 121 VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETT 180

Query: 181 ASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVVAMNK 240
           ASSGSILERTA+MTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAP+INLVVAMNK
Sbjct: 181 ASSGSILERTAKMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPNINLVVAMNK 240

Query: 241 SESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDNG 300
           SESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELY AIKPVLEKYMLKVISDNG
Sbjct: 241 SESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYGAIKPVLEKYMLKVISDNG 300

Query: 301 GLDRFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRD 360
           G DRFHQVT+SNED+DT DSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRD
Sbjct: 301 GRDRFHQVTNSNEDDDTLDSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRD 360

Query: 361 SIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVLDAEEIAR 420
           SIRA+FEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQS+KNLMRAHVVALVLDAEEIAR
Sbjct: 361 SIRAEFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSTKNLMRAHVVALVLDAEEIAR 420

Query: 421 ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILKAVPEEIQTVIPQV 480
           ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKIL+AVPEEIQTVIPQV
Sbjct: 421 ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILEAVPEEIQTVIPQV 480

Query: 481 TGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLSTARLNRWLRKVMSRHSWKDLSAQPK 540
           TGIPVIFISALEGRGRLEVMHQV+ETYEKWC RLSTARLNRWLRKVMSRHSWKD SAQPK
Sbjct: 481 TGIPVIFISALEGRGRLEVMHQVIETYEKWCTRLSTARLNRWLRKVMSRHSWKDQSAQPK 540

Query: 541 VKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKSLKEDFNLGGIPIRIMQRVEPKKTVD 600
           VKYFTQVKARPPTFVAFVSGK RLSDTDIRFLTKSLKEDFNLGGIPIRIMQRV PKKTVD
Sbjct: 541 VKYFTQVKARPPTFVAFVSGKIRLSDTDIRFLTKSLKEDFNLGGIPIRIMQRVVPKKTVD 600

Query: 601 G 602
           G
Sbjct: 601 G 601

BLAST of PI0025529 vs. NCBI nr
Match: XP_038903770.1 (GTPase Der isoform X1 [Benincasa hispida])

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 550/601 (91.51%), Postives = 568/601 (94.51%), Query Frame = 0

Query: 1   MSRLWVPLLSQRRRFIGNGVFHIVETSRRICSRTPSPNFLTDLVFVKSGWNTCISTARLL 60
           MSRLWVPLLSQRRRFIGN +FHI  +SRRI S TPSPNFL+  VFVKSGWN+CI+ ARLL
Sbjct: 1   MSRLWVPLLSQRRRFIGNSIFHIGASSRRISSSTPSPNFLSGSVFVKSGWNSCIANARLL 60

Query: 61  LPLPAVHSARGFCGVPENGLSEKCLNDSASDGKVVANKVQGVQRKSVDFTKIPINMLPTV 120
           LPL A H ARGFCGVPENG SEKCLND+ASD  V+ANKVQGVQR SVDFTKIPINMLPTV
Sbjct: 61  LPLGAAHIARGFCGVPENGQSEKCLNDNASD--VIANKVQGVQRNSVDFTKIPINMLPTV 120

Query: 121 VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA 180
           VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA
Sbjct: 121 VLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETA 180

Query: 181 ASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVVAMNK 240
            SSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVVAMNK
Sbjct: 181 TSSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVVAMNK 240

Query: 241 SESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVISDNG 300
           SESLFD++ T+LAA LEAQRLGFGDPVPISAETGLGMQELY AIKPVLEKYMLKVISDNG
Sbjct: 241 SESLFDSNGTILAAALEAQRLGFGDPVPISAETGLGMQELYCAIKPVLEKYMLKVISDNG 300

Query: 301 GLDRFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRD 360
           GLDRFHQVT SNEDEDTQDSK KLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRD
Sbjct: 301 GLDRFHQVTGSNEDEDTQDSKVKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRD 360

Query: 361 SIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVLDAEEIAR 420
           S+RA+FEFEGRTIYLVDTAGW+HRTKEEKGP SLSVMQS+KNLMRAHVVALVLDAEEIAR
Sbjct: 361 SVRAEFEFEGRTIYLVDTAGWMHRTKEEKGPASLSVMQSTKNLMRAHVVALVLDAEEIAR 420

Query: 421 ERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILKAVPEEIQTVIPQV 480
           ERRSMKHSEVVIARRAVEEGR LVVIVNKMDLMRGKK FASYEKIL+AVPEEIQTVIPQV
Sbjct: 421 ERRSMKHSEVVIARRAVEEGRGLVVIVNKMDLMRGKKFFASYEKILEAVPEEIQTVIPQV 480

Query: 481 TGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLSTARLNRWLRKVMSRHSWKDLSAQPK 540
           TGIPVIFISALEG+GRLEVM QV+ETYEKWC RLSTARLNRWLRKVM RHSWKD SAQ K
Sbjct: 481 TGIPVIFISALEGKGRLEVMRQVIETYEKWCTRLSTARLNRWLRKVMGRHSWKDQSAQAK 540

Query: 541 VKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKSLKEDFNLGGIPIRIMQRVEPKKTVD 600
           VKYFTQVKARPPTFVAFVSGKTRL+DTDIRFLTKSLKEDFNLGGIPIRIMQR   KKT D
Sbjct: 541 VKYFTQVKARPPTFVAFVSGKTRLTDTDIRFLTKSLKEDFNLGGIPIRIMQRAVLKKTAD 599

Query: 601 G 602
           G
Sbjct: 601 G 599

BLAST of PI0025529 vs. NCBI nr
Match: XP_022984952.1 (uncharacterized protein LOC111483067 [Cucurbita maxima])

HSP 1 Score: 1021.1 bits (2639), Expect = 3.9e-294
Identity = 532/626 (84.98%), Postives = 553/626 (88.34%), Query Frame = 0

Query: 1   MSRLWVPLLSQRRRFIGNGVFHIVETSRRICSRTPSPNFLTDLV---------------- 60
           MSRLW PLLSQRRR IGNGV  IV  SR     +  PNFL+  V                
Sbjct: 1   MSRLWFPLLSQRRRVIGNGVSDIVGRSRTTSFSSLLPNFLSGSVLCNGGHTPSNFSTLPK 60

Query: 61  ---------FVKSGWNTCISTARLLLPLPAVHSARGFCGVPENGLSEKCLNDSASDGKVV 120
                    FVKSGWN+ IS+ RLLLPL AVH ARGFCGV ENG SEK LND ASDG VV
Sbjct: 61  PLSEKCNHSFVKSGWNSGISSTRLLLPLAAVHIARGFCGVSENGHSEKFLNDKASDGDVV 120

Query: 121 ANKVQGVQRKSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTR 180
           AN VQ V + SVDFTKIPINMLPTVVLVGRPNVGKSALFNRFI+RREALVYNTPDDHVTR
Sbjct: 121 ANIVQAVHKNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180

Query: 181 DIREGVAKLGDLRFVVLDSSGLETAASSGSILERTARMTENVLLKSQLAIFLIDARAGLH 240
           DIREGVAKLGDLRFVVLDSSGLETA SSGSILERTARMTE VLLK QLAIFLIDARAGLH
Sbjct: 181 DIREGVAKLGDLRFVVLDSSGLETAVSSGSILERTARMTEKVLLKCQLAIFLIDARAGLH 240

Query: 241 PFDLEVGKWLRRHAPDINLVVAMNKSESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGL 300
           PFDLEVGKWLRR+ PDIN++VAMNKSESL D+S TLLAAG EA+RLGFGDPVPISAETGL
Sbjct: 241 PFDLEVGKWLRRNVPDINILVAMNKSESLIDSSGTLLAAGFEAERLGFGDPVPISAETGL 300

Query: 301 GMQELYWAIKPVLEKYMLKVISDNGGLDRFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVG 360
           GM +LY AIKPVLEKYMLKVI+DNGGLDRFHQVT SNEDEDTQDSK +LQLAIVGRPNVG
Sbjct: 301 GMHDLYLAIKPVLEKYMLKVINDNGGLDRFHQVTGSNEDEDTQDSKVQLQLAIVGRPNVG 360

Query: 361 KSTLLNTLLQSDRVLVGPEAGLTRDSIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLS 420
           KSTLLNTLLQSDRVLVGPEAGLTRDS+RAQFEFEGRTIYLVDTAGWLHRTKEEKGP SLS
Sbjct: 361 KSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPSSLS 420

Query: 421 VMQSSKNLMRAHVVALVLDAEEIARERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRG 480
           VMQS+KNLMRAHVVALVLDAEEIARERRSMKH+EVVIARRAVEEGR LV+IVNKMDLMRG
Sbjct: 421 VMQSTKNLMRAHVVALVLDAEEIARERRSMKHAEVVIARRAVEEGRGLVIIVNKMDLMRG 480

Query: 481 KKNFASYEKILKAVPEEIQTVIPQVTGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLS 540
           +KN ASYEKIL+AVPEEIQTVIPQVTGIPVIFISALEGRGRL VM QVVETYEKWC+RLS
Sbjct: 481 RKNSASYEKILEAVPEEIQTVIPQVTGIPVIFISALEGRGRLAVMRQVVETYEKWCLRLS 540

Query: 541 TARLNRWLRKVMSRHSWKDLSAQPKVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKS 600
           TARLNRWLRKVMSRHSWKD SAQPKVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKS
Sbjct: 541 TARLNRWLRKVMSRHSWKDQSAQPKVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKS 600

Query: 601 LKEDFNLGGIPIRIMQRVEPKKTVDG 602
           LKEDFNLGGIPIRIMQR  PKKT DG
Sbjct: 601 LKEDFNLGGIPIRIMQRAVPKKTDDG 626

BLAST of PI0025529 vs. TAIR 10
Match: AT5G39960.1 (GTP binding;GTP binding )

HSP 1 Score: 695.7 bits (1794), Expect = 3.5e-200
Identity = 354/545 (64.95%), Postives = 432/545 (79.27%), Query Frame = 0

Query: 57  ARLLLPLPAVHSARGFCGVPENGLSEKCLNDSASDGKVVANKVQGVQRKSVDFTKIPINM 116
           + L+  L  V S  GF  V +        ++  +  K+V  K +   +K +DFTKI  N+
Sbjct: 64  SNLISDLNLVRSTNGFATVSDE-------SNVDNGEKLVDKKPKIFYKKPIDFTKIDANL 123

Query: 117 LPTVVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAKLGDLRFVVLDSSG 176
           LPTV+L+GRPNVGKSAL+NR I+RREALVYNTPDDHVTRDIREG+AKLGDLRF VLDS+G
Sbjct: 124 LPTVMLMGRPNVGKSALYNRLIRRREALVYNTPDDHVTRDIREGIAKLGDLRFNVLDSAG 183

Query: 177 LETAASSGSILERTARMTENVLLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVV 236
           +ET  SSG+IL RT  MT NVL ++Q A+ +ID RAGLHP DLEVGKWLR+HAP I  +V
Sbjct: 184 IETEVSSGTILGRTTAMTANVLARTQFAVLIIDVRAGLHPLDLEVGKWLRKHAPQIKPIV 243

Query: 237 AMNKSESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVI 296
            MNKSES+     +L     EA  LGFG+P+ ISAETGLGM  LY  ++P+LE Y ++++
Sbjct: 244 VMNKSESI----GSLDEVASEALALGFGEPIAISAETGLGMTTLYEVLRPLLEDYTVEML 303

Query: 297 SDNGGLDRFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAG 356
           +D G  D    +T  N  ++  +SK  LQLAIVG+PNVGKSTLLN LL+ +RVLVGPEAG
Sbjct: 304 NDIGSQD--DVLTDENLSDEIDESKLPLQLAIVGKPNVGKSTLLNALLEEERVLVGPEAG 363

Query: 357 LTRDSIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVLDAE 416
           LTRD++R QFEF+GRT+YLVDTAGWL RT+ +KGP SLS+MQS K+LMRAHV+ALVLDAE
Sbjct: 364 LTRDAVRVQFEFQGRTVYLVDTAGWLERTERDKGPASLSIMQSRKSLMRAHVIALVLDAE 423

Query: 417 EIARERRSMKHSEVVIARRAVEEGRALVVIVNKMDLMRGKKNFASYEKILKAVPEEIQTV 476
           EI + + SM HSEVVIARRAVEEGR LVVIVNKMD +RG++N   Y+KI +AVP EIQTV
Sbjct: 424 EIIKAKCSMTHSEVVIARRAVEEGRGLVVIVNKMDRLRGRENSEMYKKIKEAVPIEIQTV 483

Query: 477 IPQVTGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLSTARLNRWLRKVMSRHSWKDLS 536
           IPQ+TGIPV+FISALEGRGR+EVM +V +TY++WC RLST RLNRWLRKVMSRHSWKD +
Sbjct: 484 IPQITGIPVVFISALEGRGRMEVMKEVTDTYKRWCSRLSTGRLNRWLRKVMSRHSWKDTA 543

Query: 537 AQPKVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKSLKEDFNLGGIPIRIMQRVEPK 596
           +QPK+K+FTQVKARPPTFVAFVSGKT+L ++DIRFLT+SLKEDF+LGG PIRI+QRV P+
Sbjct: 544 SQPKIKFFTQVKARPPTFVAFVSGKTQLLESDIRFLTRSLKEDFDLGGTPIRIIQRVIPR 595

Query: 597 KTVDG 602
               G
Sbjct: 604 APPSG 595

BLAST of PI0025529 vs. TAIR 10
Match: AT3G12080.1 (GTP-binding family protein )

HSP 1 Score: 214.9 bits (546), Expect = 1.8e-55
Identity = 174/571 (30.47%), Postives = 284/571 (49.74%), Query Frame = 0

Query: 85  LNDSASDGKVVANKVQGVQRKSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIKRREAL 144
           + D   +GK    K    +R + +  +IP ++L  V +VGRPNVGKSALFNR +    A+
Sbjct: 129 IEDETIEGKETRRK---GKRLAKNTQQIPEHLLQRVAIVGRPNVGKSALFNRLVGENRAI 188

Query: 145 VYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETAASSGS-ILER-------------- 204
           V + P   VTRD   G +  GD  FVV+D+ G+ T + S S ++E               
Sbjct: 189 VVDEPG--VTRDRLYGRSYWGDQEFVVVDTGGVMTVSKSPSGVMEELNVSTTIGMEGIPL 248

Query: 205 ------TARMTENV-------LLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVV 264
                  ARM   +       + +S + IF++D +AG    D+E+  WLR++     +++
Sbjct: 249 SSREAAIARMPSMIEKQATAAVDESAVIIFVVDGQAGPSGADVEIADWLRKYYSHKYIIL 308

Query: 265 AMNKSESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVI 324
           A+NK ES        L    E   LGF  P+PISA +G G  EL            L ++
Sbjct: 309 AVNKCESPRKG----LMQASEFWSLGF-TPIPISALSGTGTGEL------------LDLV 368

Query: 325 SDNGGLDRFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAG 384
               GL +   + +  E+E+         +AI+GRPNVGKS++LN L++ DR +V P +G
Sbjct: 369 C--SGLIKLEIMENIEEEEEENYIPA---IAIIGRPNVGKSSILNALVREDRTIVSPVSG 428

Query: 385 LTRDSIRAQFEF-EGRTIYLVDTAGWLHRTKEEKGPGS----LSVMQSSKNLMRAHVVAL 444
            TRD+I A+F   +G    L+DTAG + +       GS    +SV ++ + + R+ VVAL
Sbjct: 429 TTRDAIDAEFTGPDGEKFRLIDTAG-IRKKSSVASSGSTTEAMSVNRAFRAIRRSDVVAL 488

Query: 445 VLDAEEIARERRSMKHSEVVIARRAVEEGRALVVIVNKMDLM--RGKKNFASYEKILKAV 504
           V++A     E+      ++ IA R   EG+  +V+VNK D +  + ++  A YE      
Sbjct: 489 VIEAMACITEQ------DLKIAERIEREGKGCLVVVNKWDTIPNKNQETAAHYE------ 548

Query: 505 PEEIQTVIPQVTGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLSTARLNRWLRKVMSR 564
            ++++  +  +   P+++ +A+ G     ++       ++   RLSTA LN+ +R+ ++ 
Sbjct: 549 -DDVREKLRSLKWAPIVYSTAITGHSVDNIVVAAATVQKERSRRLSTAILNQVIREAVAF 608

Query: 565 HSWKDL--SAQPKVKYFTQVKARPPTFVAFVSGKTRLSDTDIRFLTKSLKEDFNLGGIPI 619
            S        + +V Y TQ   RPPTFV FV+     SDT  R++ K L+ D    G PI
Sbjct: 609 KSPPRTRGGKRGRVYYCTQAAIRPPTFVFFVNDAKLFSDTYRRYMEKQLRTDAGFAGTPI 658

BLAST of PI0025529 vs. TAIR 10
Match: AT3G12080.2 (GTP-binding family protein )

HSP 1 Score: 177.6 bits (449), Expect = 3.2e-44
Identity = 146/479 (30.48%), Postives = 241/479 (50.31%), Query Frame = 0

Query: 85  LNDSASDGKVVANKVQGVQRKSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIKRREAL 144
           + D   +GK    K    +R + +  +IP ++L  V +VGRPNVGKSALFNR +    A+
Sbjct: 129 IEDETIEGKETRRK---GKRLAKNTQQIPEHLLQRVAIVGRPNVGKSALFNRLVGENRAI 188

Query: 145 VYNTPDDHVTRDIREGVAKLGDLRFVVLDSSGLETAASSGS-ILER-------------- 204
           V + P   VTRD   G +  GD  FVV+D+ G+ T + S S ++E               
Sbjct: 189 VVDEPG--VTRDRLYGRSYWGDQEFVVVDTGGVMTVSKSPSGVMEELNVSTTIGMEGIPL 248

Query: 205 ------TARMTENV-------LLKSQLAIFLIDARAGLHPFDLEVGKWLRRHAPDINLVV 264
                  ARM   +       + +S + IF++D +AG    D+E+  WLR++     +++
Sbjct: 249 SSREAAIARMPSMIEKQATAAVDESAVIIFVVDGQAGPSGADVEIADWLRKYYSHKYIIL 308

Query: 265 AMNKSESLFDNSCTLLAAGLEAQRLGFGDPVPISAETGLGMQELYWAIKPVLEKYMLKVI 324
           A+NK ES        L    E   LGF  P+PISA +G G  EL            L ++
Sbjct: 309 AVNKCESPRKG----LMQASEFWSLGF-TPIPISALSGTGTGEL------------LDLV 368

Query: 325 SDNGGLDRFHQVTSSNEDEDTQDSKTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAG 384
               GL +   + +  E+E+         +AI+GRPNVGKS++LN L++ DR +V P +G
Sbjct: 369 C--SGLIKLEIMENIEEEEEENYIPA---IAIIGRPNVGKSSILNALVREDRTIVSPVSG 428

Query: 385 LTRDSIRAQFEF-EGRTIYLVDTAGWLHRTKEEKGPGS----LSVMQSSKNLMRAHVVAL 444
            TRD+I A+F   +G    L+DTAG + +       GS    +SV ++ + + R+ VVAL
Sbjct: 429 TTRDAIDAEFTGPDGEKFRLIDTAG-IRKKSSVASSGSTTEAMSVNRAFRAIRRSDVVAL 488

Query: 445 VLDAEEIARERRSMKHSEVVIARRAVEEGRALVVIVNKMDLM--RGKKNFASYEKILKAV 504
           V++A     E+      ++ IA R   EG+  +V+VNK D +  + ++  A YE      
Sbjct: 489 VIEAMACITEQ------DLKIAERIEREGKGCLVVVNKWDTIPNKNQETAAHYE------ 548

Query: 505 PEEIQTVIPQVTGIPVIFISALEGRGRLEVMHQVVETYEKWCMRLSTARLNRWLRKVMS 529
            ++++  +  +   P+++ +A+ G     ++       ++   RLSTA LN+ +R+ ++
Sbjct: 549 -DDVREKLRSLKWAPIVYSTAITGHSVDNIVVAAATVQKERSRRLSTAILNQVIREAVA 566

BLAST of PI0025529 vs. TAIR 10
Match: AT1G78010.1 (tRNA modification GTPase, putative )

HSP 1 Score: 64.7 bits (156), Expect = 3.0e-10
Identity = 52/156 (33.33%), Postives = 87/156 (55.77%), Query Frame = 0

Query: 306 HQVTSSNED-EDTQDS-------KTKLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGL 365
           +++TS ++D E   D+       ++ LQ+AIVGRPNVGKS+LLN   +S+R +V   AG 
Sbjct: 292 NKITSMSQDVESALDTANYDKLLQSGLQIAIVGRPNVGKSSLLNAWSKSERAIVTEVAGT 351

Query: 366 TRDSIRAQFEFEGRTIYLVDTAGWLHRTKE--EKGPGSLSVMQSSKNLMRAHVVALVLDA 425
           TRD + A     G  I L+DTAG +  T +  EK    + V +S      A V+ + + A
Sbjct: 352 TRDVVEANVTVRGVPITLLDTAG-IRETNDIVEK----IGVERSETAAKVADVIIMAVSA 411

Query: 426 EEIARERRSMKHSEVVIARRAVEEGRALVVIVNKMD 452
            E   E    + +E++   R ++  + +++++NK+D
Sbjct: 412 VEGWTE----EDTELL---RKIQSDKPMILVMNKID 435

BLAST of PI0025529 vs. TAIR 10
Match: AT5G11480.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 51.2 bits (121), Expect = 3.5e-06
Identity = 37/135 (27.41%), Postives = 64/135 (47.41%), Query Frame = 0

Query: 104 RKSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTRDIREGVAK 163
           + SV     P + LP   LVGR NVGKS+L N  + RR+ L   +     T+ I     +
Sbjct: 122 KSSVKTEDCPADGLPEFALVGRSNVGKSSLLNSLV-RRKRLALTSKKPGKTQCINH--FR 181

Query: 164 LGDLRFVVLDSSGLETAASSGSILERTARMTENVLLKSQLAI---FLIDARAGLHPFDLE 223
           + D ++ ++D  G   A++   + +   + T++  L     +    L+DA   + P DLE
Sbjct: 182 IND-KWYLVDLPGYGYASAPHELKQDWNKFTKDYFLNRSTLVSVFLLVDASIPVKPIDLE 241

Query: 224 VGKWLRRHAPDINLV 236
              WL ++   + L+
Sbjct: 242 YASWLGQNQVPMTLI 252