PI0025422 (gene) Melon (PI 482460) v1

Overview


Name	PI0025422
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Retrotransposon protein
Location	chr06: 15078853 .. 15080511 (-)
RNA-Seq Expression	PI0025422
Synteny	PI0025422

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGCTTCACGTCCTAGCACACGATGTGAAGAACCGAGTGGTCCAGCAGGAGTTCGTGCGGTCCGGTGAGACAATTTCAAGGCATTTCAACATGGTGTTGGTGGCCGTTGTACGTCTTCATGACGAGCTGGTAAAAAACCACAACCAGTACCGAACAACTGCACAAATCCCCGCTGGCGTTGTTTCGAGGTTCGCCTCCTTCGATCTTTACCGTGTATAAATTTTGTAATGGCATGATCTAACAAGGCATTGTTCTTGTGAGGACTAGAATTGCCTTGGTGCACTAGATGGCACATACATCAAGGTGAACGTGACAGCCACCGAGCGGGCATCGTATAGAATGCGGAAAGGGGAAGTTGCAACGAACGTCCTGGGCGTCTGTGACACAAAGGGAAGTTTTGTGTACGTACTCGCTGGGTGGGAAGGGTCGGTTGCTGACTCGAGCATCCTTCGCGATGCTATTTCAAGACAAAATGGATTGAAGGTTCCCAAGGGTAAATGTGTTCTCTCAATAAGTTAGAAATATTTGTGAATAACCTTTCCTTGCAACCAAACTTAACAACATATACCATTAGGGTACTACTACTTATGTGATGCGGGTTACCCCAACGTCGAGGGATTCCTGGCTCCTTATAGAGGGTAGAGGTACCACCTGCAGAAGTGGCGTGGCGCAGGGAATTCTCCAACAACGGTGAAAGAATTCTTTAACATGAAGCATTCTTCTGCAAGAAACGTCATTGAGAGAGCATTCGGTCTACTTAAAGGTCGATGGGCAATTCTTTGCGGGAAGTCGTACTATCACGTGGAAGTACAATGTCGCACAATACTGACATGTTGTCTACTGCACAACTTGATTAATAGGGAGATGACGAGTTCTGAGGTAATAGAGGACGTCGACGAAGTGGACTCAACATATGCAACGACGAGAGGTGACGACATTCAGTACGTTGAAAGCTCGGACGAATGGAGTAAGTGGAGGGATGATAAAGCGGAGGAGATGTTCAATGAATGGCAACTGTGGAATCAGTAGTTACCCTTCTATGCCAATGGACTTTACTTCTGCATAACTTTACTATGTACTTGCTAATGTTTTTTTAAATTGTCATCGTTATGTATGAAATTACCCATGATATGTATTAGATGTTTTTAAATTTTTTGTTAATCCTTCATTTAATATATATCTTTTGTTTCATGTTTGGTAGAATGGCAAGTTCGTCGAGGGCCCCCAAGCATACGTGGACCAGAGAAGAAGAGTCAACCCTCGTCGAATGTTTGGTGGAGTTGGTATCCATGGGGGGGGGGGGTGGAAATCGGACAACGGCACATTCTGACCCGGTTACTTGGCAATGATTATGCGAATGATGTCGAAAAAGCTCCCAGGGTGTAGCATACAGGGGACTACGACTATTGACTGTCGGATCAAGACACTTAAGCGCACCTTTCAAGCCATATCGGAGATGCAGGGCCCTACCTGCAGTGGATTCGGCTAGAATGACAAGGTCAAATGCATAATTGCAGAGAAGGACCTCTTTGACAATTGGGTGAAGGTACGTATTTTCATGGTTATCTAGTATTGGCCCTATTAAATTATTTTGCGTTCCCGTAATTAATGAATCTAATAAAGTGGTTGTATTTTTCTTTTGCACAGAGTCACCCA

mRNA sequence

ATGTTGCTTCACGTCCTAGCACACGATGTGAAGAACCGAGTGGTCCAGCAGGAGTTCGTGCGGTCCGGTGAGACAATTTCAAGGCATTTCAACATGGTGTTGGTGGCCGTTGTACGTCTTCATGACGAGCTGGTAAAAAACCACAACCAGTACCGAACAACTGCACAAATCCCCGCTGGCGTTGTTTCGAGAATGGCAAGTTCGTCGAGGGCCCCCAAGCATACGTGGACCAGAGAAGAAGAGTCAACCCTCGTCGAATGTTTGGTGGAGTTGGTATCCATGGGGGGGGGGGGTGGAAATCGGACAACGGCACATTCTGACCCGGTTACTTGGCAATGATTATGCGAATGATGTCGAAAAAGCTCCCAGGGTGTAGCATACAGGGGACTACGACTATTGACTGTCGGATCAAGACACTTAAGCGCACCTTTCAAGCCATATCGGAGATGCAGGGCCCTACCTGCAGTGGATTCGGCTAGAATGACAAGGTCAAATGCATAATTGCAGAGAAGGACCTCTTTGACAATTGGGTGAAGAGTCACCCA

Coding sequence (CDS)

ATGTTGCTTCACGTCCTAGCACACGATGTGAAGAACCGAGTGGTCCAGCAGGAGTTCGTGCGGTCCGGTGAGACAATTTCAAGGCATTTCAACATGGTGTTGGTGGCCGTTGTACGTCTTCATGACGAGCTGGTAAAAAACCACAACCAGTACCGAACAACTGCACAAATCCCCGCTGGCGTTGTTTCGAGAATGGCAAGTTCGTCGAGGGCCCCCAAGCATACGTGGACCAGAGAAGAAGAGTCAACCCTCGTCGAATGTTTGGTGGAGTTGGTATCCATGGGGGGGGGGGGTGGAAATCGGACAACGGCACATTCTGACCCGGTTACTTGGCAATGA

Protein sequence

MLLHVLAHDVKNRVVQQEFVRSGETISRHFNMVLVAVVRLHDELVKNHNQYRTTAQIPAGVVSRMASSSRAPKHTWTREEESTLVECLVELVSMGGGGGNRTTAHSDPVTWQ

Homology

[Close]

BLAST of PI0025422 vs. ExPASy TrEMBL
Match: A0A5D3CTT8 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5393G00050 PE=4 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.1e-19
Identity = 56/118 (47.46%), Postives = 77/118 (65.25%), Query Frame = 0


Query: 1   MLLHVLAHDVKNRVVQQEFVRSGETISRHFNMVLVAVVRLHDELVK---------NHNQY 60
           M LH+LAHD+KNRV+Q+ F+RSGETI RHFNMVL+A++RLHDEL+K            ++
Sbjct: 33  MFLHILAHDMKNRVIQRHFMRSGETIFRHFNMVLLAIIRLHDELLKKPQPVPNNCTDQRW 92

Query: 61  R-------------TTAQIPAGVVSRMASSSRAPKHTWTREEESTLVECLVELVSMGG 97
           R                 +PAG    +A++ R PKH+  +EEE+ +VECLVELV++GG
Sbjct: 93  RWFENCLDALDGTYIKVNVPAGDRPSIATTLRLPKHSCIKEEETWMVECLVELVNVGG 150

[Close]

BLAST of PI0025422 vs. ExPASy TrEMBL
Match: A0A5A7SW62 (Myb_DNA-bind_3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold261G00210 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 8.2e-15
Identity = 46/96 (47.92%), Postives = 64/96 (66.67%), Query Frame = 0


Query: 1   MLLHVLAHDVKNRVVQQEFVRSGETISRHFNMVLVAVVRLHDELVKNHNQYRTTAQIPAG 60
           + LH++AHDVKNRV ++ F RSGET+SRHFN+VL  V+RLH+ L+K  +    +      
Sbjct: 22  IFLHIVAHDVKNRVARRHFARSGETVSRHFNVVLNVVLRLHEILLKQPDSVTHSCSHEKW 81

Query: 61  VVSRMAS-SSRAPKHTWTREEESTLVECLVELVSMG 96
              +MAS +S+  KH WT  E+  LVECL++LV  G
Sbjct: 82  RWFQMASINSKVTKHWWTTIEDEALVECLLQLVEKG 117

[Close]

BLAST of PI0025422 vs. ExPASy TrEMBL
Match: A0A5D3DTL0 (Myb_DNA-bind_3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold260G00340 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 8.2e-15
Identity = 46/96 (47.92%), Postives = 64/96 (66.67%), Query Frame = 0


Query: 1   MLLHVLAHDVKNRVVQQEFVRSGETISRHFNMVLVAVVRLHDELVKNHNQYRTTAQIPAG 60
           + LH++AHDVKNRV ++ F RSGET+SRHFN+VL  V+RLH+ L+K  +    +      
Sbjct: 22  IFLHIVAHDVKNRVARRHFARSGETVSRHFNVVLNVVLRLHEILLKQPDSVTHSCSHEKW 81

Query: 61  VVSRMAS-SSRAPKHTWTREEESTLVECLVELVSMG 96
              +MAS +S+  KH WT  E+  LVECL++LV  G
Sbjct: 82  RWFQMASINSKVTKHWWTTIEDEALVECLLQLVEKG 117

[Close]

BLAST of PI0025422 vs. ExPASy TrEMBL
Match: A0A5D3DA20 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold708G00510 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.8e-14
Identity = 60/175 (34.29%), Postives = 79/175 (45.14%), Query Frame = 0


Query: 1   MLLHVLAHDVKNRVVQQEFVRSGETISRHFNMVLVAVVRLHDELVK--NHN--------- 60
           M LH+LAHDVKNRV+Q++F+RSGETI  HFNMVL+AV+RLHDEL+K   HN         
Sbjct: 4   MFLHILAHDVKNRVIQRKFMRSGETIFHHFNMVLLAVIRLHDELLKKPQHNCLGALDGMY 63

Query: 61  ---QYRTTA---------------QIPAG------------------------------- 96
                  TA               ++P G                               
Sbjct: 64  IKVNIPATADSCILRDAILRPNVRKVPKGYYYLVDVGYLNAEGFLAPYRGQRYHLQKWRG 123

[Close]

BLAST of PI0025422 vs. ExPASy TrEMBL
Match: A0A5A7TXW1 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold298G00370 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 4.5e-13
Identity = 38/46 (82.61%), Postives = 45/46 (97.83%), Query Frame = 0


Query: 1   MLLHVLAHDVKNRVVQQEFVRSGETISRHFNMVLVAVVRLHDELVK 47
           M LH+LAHDVKNRV+Q+EF+RSGETISRHFNMVL+AV+RLHDEL+K
Sbjct: 99  MFLHILAHDVKNRVIQREFMRSGETISRHFNMVLLAVIRLHDELLK 144

[Close]

BLAST of PI0025422 vs. NCBI nr
Match: KAA0033168.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa] >TYK15357.1 putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 105.9 bits (263), Expect = 2.3e-19
Identity = 56/118 (47.46%), Postives = 77/118 (65.25%), Query Frame = 0


Query: 1   MLLHVLAHDVKNRVVQQEFVRSGETISRHFNMVLVAVVRLHDELVK---------NHNQY 60
           M LH+LAHD+KNRV+Q+ F+RSGETI RHFNMVL+A++RLHDEL+K            ++
Sbjct: 33  MFLHILAHDMKNRVIQRHFMRSGETIFRHFNMVLLAIIRLHDELLKKPQPVPNNCTDQRW 92

Query: 61  R-------------TTAQIPAGVVSRMASSSRAPKHTWTREEESTLVECLVELVSMGG 97
           R                 +PAG    +A++ R PKH+  +EEE+ +VECLVELV++GG
Sbjct: 93  RWFENCLDALDGTYIKVNVPAGDRPSIATTLRLPKHSCIKEEETWMVECLVELVNVGG 150

[Close]

BLAST of PI0025422 vs. NCBI nr
Match: KAA0033487.1 (uncharacterized protein E6C27_scaffold261G00210 [Cucumis melo var. makuwa])

HSP 1 Score: 89.7 bits (221), Expect = 1.7e-14
Identity = 46/96 (47.92%), Postives = 64/96 (66.67%), Query Frame = 0


Query: 1   MLLHVLAHDVKNRVVQQEFVRSGETISRHFNMVLVAVVRLHDELVKNHNQYRTTAQIPAG 60
           + LH++AHDVKNRV ++ F RSGET+SRHFN+VL  V+RLH+ L+K  +    +      
Sbjct: 22  IFLHIVAHDVKNRVARRHFARSGETVSRHFNVVLNVVLRLHEILLKQPDSVTHSCSHEKW 81

Query: 61  VVSRMAS-SSRAPKHTWTREEESTLVECLVELVSMG 96
              +MAS +S+  KH WT  E+  LVECL++LV  G
Sbjct: 82  RWFQMASINSKVTKHWWTTIEDEALVECLLQLVEKG 117

[Close]

BLAST of PI0025422 vs. NCBI nr
Match: TYK26842.1 (uncharacterized protein E5676_scaffold260G00340 [Cucumis melo var. makuwa])

HSP 1 Score: 89.7 bits (221), Expect = 1.7e-14
Identity = 46/96 (47.92%), Postives = 64/96 (66.67%), Query Frame = 0


Query: 1   MLLHVLAHDVKNRVVQQEFVRSGETISRHFNMVLVAVVRLHDELVKNHNQYRTTAQIPAG 60
           + LH++AHDVKNRV ++ F RSGET+SRHFN+VL  V+RLH+ L+K  +    +      
Sbjct: 22  IFLHIVAHDVKNRVARRHFARSGETVSRHFNVVLNVVLRLHEILLKQPDSVTHSCSHEKW 81

Query: 61  VVSRMAS-SSRAPKHTWTREEESTLVECLVELVSMG 96
              +MAS +S+  KH WT  E+  LVECL++LV  G
Sbjct: 82  RWFQMASINSKVTKHWWTTIEDEALVECLLQLVEKG 117

[Close]

BLAST of PI0025422 vs. NCBI nr
Match: TYK20320.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 88.6 bits (218), Expect = 3.8e-14
Identity = 60/175 (34.29%), Postives = 79/175 (45.14%), Query Frame = 0


Query: 1   MLLHVLAHDVKNRVVQQEFVRSGETISRHFNMVLVAVVRLHDELVK--NHN--------- 60
           M LH+LAHDVKNRV+Q++F+RSGETI  HFNMVL+AV+RLHDEL+K   HN         
Sbjct: 4   MFLHILAHDVKNRVIQRKFMRSGETIFHHFNMVLLAVIRLHDELLKKPQHNCLGALDGMY 63

Query: 61  ---QYRTTA---------------QIPAG------------------------------- 96
                  TA               ++P G                               
Sbjct: 64  IKVNIPATADSCILRDAILRPNVRKVPKGYYYLVDVGYLNAEGFLAPYRGQRYHLQKWRG 123

[Close]

BLAST of PI0025422 vs. NCBI nr
Match: KAA0057637.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 84.0 bits (206), Expect = 9.3e-13
Identity = 38/46 (82.61%), Postives = 45/46 (97.83%), Query Frame = 0


Query: 1  MLLHVLAHDVKNRVVQQEFVRSGETISRHFNMVLVAVVRLHDELVK 47
          M LH+LAHDVKNRV+Q+EF+RSGETISRHFNMVL+AV+RLHDEL+K
Sbjct: 33 MFLHILAHDVKNRVIQREFMRSGETISRHFNMVLLAVIRLHDELLK 78

[Close]

BLAST of PI0025422 vs. TAIR 10
Match: AT5G41980.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G43722.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 44.7 bits (104), Expect = 5.8e-05
Identity = 21/53 (39.62%), Postives = 34/53 (64.15%), Query Frame = 0


Query: 1   MLLHVLAHDVKNRVVQQEFVRSGETISRHFNMVLVAVVRLHDELVKNHNQYRT 54
           + L ++ H+++ R VQ+ F  SGETISRHFN VL AV+ +  +  + ++   T
Sbjct: 80  IFLFIIGHNLRTRAVQELFCYSGETISRHFNNVLNAVIAISKDFFQPNSNSDT 132

The following BLAST results are available for this feature:

BLAST of PI0025422 vs.


Match Name	E-value	Identity	Description

BLAST of PI0025422 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CTT8	1.1e-19	47.46	Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A5A7SW62	8.2e-15	47.92	Myb_DNA-bind_3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A5D3DTL0	8.2e-15	47.92	Myb_DNA-bind_3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A5D3DA20	1.8e-14	34.29	Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A5A7TXW1	4.5e-13	82.61	Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of PI0025422 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0033168.1	2.3e-19	47.46	putative nuclease HARBI1 [Cucumis melo var. makuwa] >TYK15357.1 putative nucleas...	[more]
KAA0033487.1	1.7e-14	47.92	uncharacterized protein E6C27_scaffold261G00210 [Cucumis melo var. makuwa]	[more]
TYK26842.1	1.7e-14	47.92	uncharacterized protein E5676_scaffold260G00340 [Cucumis melo var. makuwa]	[more]
TYK20320.1	3.8e-14	34.29	retrotransposon protein [Cucumis melo var. makuwa]	[more]
KAA0057637.1	9.3e-13	82.61	putative nuclease HARBI1 [Cucumis melo var. makuwa]	[more]

BLAST of PI0025422 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41980.1	5.8e-05	39.62	CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int...	[more]

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0025422.1	PI0025422.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding